Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003354

Physical Location & Seq

Genomic Coordinates Forward (+)

23735447 .. 23740587

Gene Structure 5141 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1271800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1860 bp

ATGAGCGGTGGTGAAGGTAAAATGCTGGGATTAAGTGTGAAACAACACAAGCGTTCAAAAAGCTTTCCTGAGAAGAAAAGAGTGGAGGAAGATGATTTGGATGGTTCCGGTGAAGCATCAGACCTTGTAAAGCTGGATATGGGACAATTCAAGGGCCATGTTACGACCAAGAAGAAACAATCTCCTACCGTGGAAGTACACAACTCTTTAAAGCAAGAGATATTTTGTACACAGATTCTACAGCTTGAGAAAAGATTACAAGATCAATTCAAGGTCCGTCATGCTTTAGAAAACTCATTGGGTTATAGGTCTTCCATCCATCATAATACAACTGCAAGTGTGATGCCTAAGCCAGCCACAGAATTGATTAAGGAGATTGCTGTGTTAGAATTAGAAGTTGTGCATTTGGAACAATATCTTCTCTCATTGTATAGGAGAGCATTTGATGGGAAATTTTCCTCTGTTTCCCCATCTAAGAAGGATGAACGACTAAAACCAACTCTAACAAGCCCAAGAATCAGATTCCTGGAAGTTTCTGAACCTGATATGCCATTGGAGAGGGAAAACTTGGCAGTTCAATCCAGTGGTCAGTCACTTGAAAATTCACAGAAGGAATTCAATGCAATTGGAGGAGAAGAGAAACTATTAGATTCCAGTGTTCACCGTTGCCACTCCTCACTTTCGCAACGCTCTGCATTCTTAAGTAGAACTTCTCCAGAAGAGTCTTTGGCAAACACTTTGCGTTCCTGTCATTCTCAACCGTTGTCTATGATGGAGTATGCTCAGAATACTTCGTCAAATTTAATTAGTCTGGCAGAGCATCTTGGTACACGCATTGCTGATCATATTCCAGAGACACCTAACAGGCTTTCAGAGGACATGATCAAGTGCATGTCCACCATATATTGCAAGCTTTCCGAATCACTCTTGTCACATAATGGCCTTTCATCTCCCAATTCATCTTTGTCATCAACAAGTGCATTTTCACCACGAGATCAGAGTGACATGTGGAGTCCACGATGTAGGAATAATTCATCTTTTGATGTACGATTGGATAATCCTTTTCATATTGAAGGACTTAAAGAGTTTAGTGGGCCATACAGCACAATGGTTGAAGTGCCATGGATTTATAGAGATAGTAAAAAACTAGGCGATATCGAGCACTTGCTACAACATTTCAGGTCACTTATCTGTAGGTTAGAGGAAGTAGATCCAAGAAAGTTGGAAAATGATGAGAAGTTAGCGTTCTGGATTAATGTACACAATACATTGGTTATGCATGCGTATTTGGCTTATGGGGTTCCTCAAAACAATGTAAAGAGAGTATTTTTGCTTTTGAAGGCTGCTTATAACATTGGGGGTCACACAATCAGCGCAGACACAATGCAAAGCTCTATCCTTGGATGCCGGATGTCTCGCCCGGGACAGTGGCTTCGTTTGTTACTTACTCCAAGGACGAAGTTCAAGACTGGAGATGAACGGCAAGCATATGCAATTGACCATCCGGAACCCCTTCTACACTTTGCACTGTGTTCAGGAAGCCATTCTGATCCTGCGGTTCGTGTATACACACCCAAGAGGGTATATCAGGATCTGGAAGCTGCAAAGGACGAGTACATTCGAGCTACCTTTGGAGTGCGCAAGGACCACAAAATTCTTCTACCGAAGATTGTTGAGGCATTTGCAAAAAGTTCGGGTTTGTGTCCAGTTGGTGTTTTAGAGATGATCCAACAGACTTTGCCTGAATCATTGAGGAAGAGTGTCAAGAAATGCCAGCTTGGGAAACACCGCAAGAGCATCGAGTATATTCCTCACAACTTCACATTTCGGTATTTAATTTCTAAAGAGCTGGTGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF547 Lzipper-MIP1

Protein Analysis

620

Amino Acids

70.18

Weight (kDa)

8.87

Isoelectric Point (pI)

56.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lzipper-MIP1	PF14389	77 - 149	1.9e-22	Leucine-zipper of ternary complex factor MIP1
DUF547	PF04784	406 - 540	1.1e-40	Protein of unknown function, DUF547

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003354)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66600 AT5G66600 AT5G66600 AT5G66600 AT5G66600 AT5G66600 AT5G66600 AT5G66600 AT5G66600 AT5G66600 AT5G66600 AT5G66600 AT5G66600 AT5G66600 AT5G66600
fragaria_vesca	FvH4_1g14360 FvH4_1g14360 FvH4_1g14360
malus_domestica	MD02G1157100.v1.1 MD15G1271800.v1.1
prunus_persica	Prupe.7G146400_v2.0.a1
pyrus_communis	pycom02g12420 pycom15g23570
rosa_chinensis	RchiOBHm_Chr2g0103061
rosa_laevigata	RLG00000017202
rosa_multiflora	Rmu_co8442071.1_g000001 Rmu_sc0000388.1_g000014
rosa_roxburghii	Rroxscaffold_2G00139960
rosa_rugosa	Rorug02G0111700 Rorug02G0111800 Rorug02G0111900
rosa_samantha	Rh2AG159900 Rh2BG166500 Rh2CG166700 Rh2DG165500
rosa_wichuraiana	Rw2G012510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1350
Acc16I	TGCGCA	1 cut(s)	1640
AccBSI	CCGCTC	1 cut(s)	6
AccI	GTMKAC	1 cut(s)	1566
AccIII	TCCGGA	1 cut(s)	1504
AciI	CCGC	3 cut(s)	6, 1556, 1789
AclWI	GGATC	4 cut(s)	1205, 1544, 1599, 1720
AcsI	RAATTY	5 cut(s)	452, 601, 614, 799, 1653
AfaI	GTAC	6 cut(s)	198, 229, 829, 1047, 1260, 1616
AflII	CTTAAG	1 cut(s)	700
AflIII	ACRYGT	1 cut(s)	1005
AjnI	CCWGG	1 cut(s)	525
AluBI	AGCT	9 cut(s)	63, 133, 244, 913, 1392, 1601, 1625, 1777, 1849
AluI	AGCT	9 cut(s)	63, 133, 244, 913, 1392, 1601, 1625, 1777, 1849
Alw21I	GWGCWC	1 cut(s)	1164
Alw26I	GTCTC	2 cut(s)	848, 1419
AlwI	GGATC	4 cut(s)	1205, 1544, 1599, 1720
AlwNI	CAGNNNCTG	1 cut(s)	1594
Ama87I	CYCGRG	1 cut(s)	1420
Aor13HI	TCCGGA	1 cut(s)	1504
AoxI	GGCC	3 cut(s)	154, 940, 1094
ApeKI	GCWGC	2 cut(s)	1343, 1601
ApoI	RAATTY	5 cut(s)	452, 601, 614, 799, 1653
ArsI	GACNNNNNNTTYG	2 cut(s)	1416, 1448
AseI	ATTAAT	1 cut(s)	1254
Asp700I	GAANNNNTTC	3 cut(s)	614, 1512, 1543
AspLEI	GCGC	2 cut(s)	1376, 1641
AspS9I	GGNCC	4 cut(s)	154, 274, 1094, 1645
AsuC2I	CCSGG	2 cut(s)	1421, 1422
AsuHPI	GGTGA	4 cut(s)	23, 122, 653, 978
AvaI	CYCGRG	1 cut(s)	1420
AvaII	GGWCC	2 cut(s)	274, 1645
BauI	CACGAG	1 cut(s)	990
BbsI	GAAGAC	1 cut(s)	303
Bbv12I	GWGCWC	1 cut(s)	1164
BbvI	GCAGC	2 cut(s)	1330, 1588
BccI	CCATC	7 cut(s)	95, 323, 327, 440, 478, 766, 1509
BceAI	ACGGC	1 cut(s)	1496
BcgI	CGANNNNNNTGC	4 cut(s)	1674, 1708, 1780, 1814
BciT130I	CCWGG	1 cut(s)	527
BclI	TGATCA	2 cut(s)	841, 882
BcnI	CCSGG	2 cut(s)	1421, 1422
BcoDI	GTCTC	2 cut(s)	848, 1419
BfaI	CTAG	1 cut(s)	1148
BfmI	CTRYAG	2 cut(s)	239, 1192
BfrI	CTTAAG	1 cut(s)	700
BisI	GCNGC	2 cut(s)	1344, 1602
BlsI	GCNGC	2 cut(s)	1345, 1603
Bme1390I	CCNGG	3 cut(s)	527, 1421, 1422
Bme18I	GGWCC	2 cut(s)	274, 1645
BmeT110I	CYCGRG	1 cut(s)	1420
BmgT120I	GGNCC	4 cut(s)	154, 274, 1094, 1645
BmiI	GGNNCC	3 cut(s)	106, 1302, 1509
BmrFI	CCNGG	3 cut(s)	527, 1421, 1422
BmsI	GCATC	5 cut(s)	125, 333, 829, 1394, 1806
BpiI	GAAGAC	1 cut(s)	303
BpmI	CTGGAG	2 cut(s)	699, 1491
Bpu10I	CCTNAGC	1 cut(s)	348
BpuEI	CTTGAG	1 cut(s)	266
BpuMI	CCSGG	2 cut(s)	1421, 1422
BsaJI	CCNNGG	5 cut(s)	189, 1121, 1399, 1420, 1451
BsaWI	WCCGGW	2 cut(s)	107, 1504
Bse1I	ACTGG	4 cut(s)	582, 654, 1474, 1706
Bse3DI	GCAATG	1 cut(s)	834
BseAI	TCCGGA	1 cut(s)	1504
BseBI	CCWGG	1 cut(s)	527
BseDI	CCNNGG	5 cut(s)	189, 1121, 1399, 1420, 1451
BseGI	GGATG	6 cut(s)	106, 315, 487, 1409, 1416, 1501
BseMI	GCAATG	1 cut(s)	834
BseMII	CTCAG	2 cut(s)	60, 797
BseNI	ACTGG	4 cut(s)	582, 654, 1474, 1706
BseRI	GAGGAG	2 cut(s)	645, 664
BseXI	GCAGC	2 cut(s)	1330, 1588
BseYI	CCCAGC	1 cut(s)	25
BshFI	GGCC	3 cut(s)	156, 942, 1096
BsiHKAI	GWGCWC	1 cut(s)	1164
BsiHKCI	CYCGRG	1 cut(s)	1420
BsiSI	CCGG	4 cut(s)	108, 1408, 1421, 1505
BslFI	GGGAC	2 cut(s)	156, 1437
BsmAI	GTCTC	2 cut(s)	848, 1419
BsmFI	GGGAC	2 cut(s)	156, 1437
BsmI	GAATGC	1 cut(s)	695
BsnI	GGCC	3 cut(s)	156, 942, 1096
BsoBI	CYCGRG	1 cut(s)	1420
Bsp1286I	GDGCHC	1 cut(s)	1164
Bsp13I	TCCGGA	1 cut(s)	1504
Bsp1407I	TGTACA	2 cut(s)	227, 1258
Bsp143I	GATC	8 cut(s)	262, 841, 882, 994, 1210, 1549, 1591, 1725
Bsp19I	CCATGG	1 cut(s)	1121
BspACI	CCGC	3 cut(s)	6, 1556, 1789
BspANI	GGCC	3 cut(s)	156, 942, 1096
BspCNI	CTCAG	2 cut(s)	61, 796
BspEI	TCCGGA	1 cut(s)	1504
BspLI	GGNNCC	3 cut(s)	106, 1302, 1509
BspPI	GGATC	4 cut(s)	1205, 1544, 1599, 1720
BspTI	CTTAAG	1 cut(s)	700
BsrBI	CCGCTC	1 cut(s)	6
BsrDI	GCAATG	1 cut(s)	834
BsrGI	TGTACA	2 cut(s)	227, 1258
BsrI	ACTGG	4 cut(s)	582, 654, 1474, 1706
BssECI	CCNNGG	5 cut(s)	189, 1121, 1399, 1420, 1451
BssMI	GATC	8 cut(s)	262, 841, 882, 994, 1210, 1549, 1591, 1725
BssNAI	GTATAC	1 cut(s)	1567
BssSI	CACGAG	1 cut(s)	990
BssT1I	CCWWGG	3 cut(s)	1121, 1399, 1451
Bst1107I	GTATAC	1 cut(s)	1567
Bst2BI	CACGAG	1 cut(s)	990
Bst2UI	CCWGG	1 cut(s)	527
Bst4CI	ACNGT	5 cut(s)	190, 665, 762, 1428, 1530
Bst6I	CTCTTC	3 cut(s)	630, 714, 1751
BstAFI	CTTAAG	1 cut(s)	700
BstAUI	TGTACA	2 cut(s)	227, 1258
BstC8I	GCNNGC	5 cut(s)	354, 911, 1281, 1485, 1775
BstDEI	CTNAG	4 cut(s)	69, 348, 474, 783
BstDSI	CCRYGG	2 cut(s)	189, 1121
BstF5I	GGATG	6 cut(s)	106, 315, 487, 1409, 1416, 1501
BstHHI	GCGC	2 cut(s)	1376, 1641
BstKTI	GATC	8 cut(s)	265, 844, 885, 997, 1213, 1552, 1594, 1728
BstMAI	GTCTC	2 cut(s)	848, 1419
BstMBI	GATC	8 cut(s)	262, 841, 882, 994, 1210, 1549, 1591, 1725
BstMWI	GCNNNNNNNGC	1 cut(s)	1289
BstNI	CCWGG	1 cut(s)	527
BstNSI	RCATGY	3 cut(s)	895, 1009, 1283
BstSCI	CCNGG	3 cut(s)	525, 1419, 1420
BstSFI	CTRYAG	2 cut(s)	239, 1192
BstV1I	GCAGC	2 cut(s)	1330, 1588
BstV2I	GAAGAC	1 cut(s)	303
BstX2I	RGATCY	2 cut(s)	1210, 1591
BstYI	RGATCY	2 cut(s)	1210, 1591
BstZ17I	GTATAC	1 cut(s)	1567
BsuRI	GGCC	3 cut(s)	156, 942, 1096
BtgI	CCRYGG	2 cut(s)	189, 1121
BtsCI	GGATG	6 cut(s)	106, 315, 487, 1409, 1416, 1501
BtsIMutI	CAGTG	4 cut(s)	589, 661, 1433, 1526
Cac8I	GCNNGC	5 cut(s)	354, 911, 1281, 1485, 1775
CaiI	CAGNNNCTG	1 cut(s)	1594
CfoI	GCGC	2 cut(s)	1376, 1641
Cfr13I	GGNCC	4 cut(s)	154, 274, 1094, 1645
Cfr9I	CCCGGG	1 cut(s)	1420
Csp6I	GTAC	6 cut(s)	197, 228, 828, 1046, 1259, 1615
CviAII	CATG	7 cut(s)	158, 281, 880, 892, 1006, 1122, 1280
CviQI	GTAC	6 cut(s)	197, 228, 828, 1046, 1259, 1615
DdeI	CTNAG	4 cut(s)	69, 348, 474, 783
DpnI	GATC	8 cut(s)	264, 843, 884, 996, 1212, 1551, 1593, 1727
DpnII	GATC	8 cut(s)	262, 841, 882, 994, 1210, 1549, 1591, 1725
DraI	TTTAAA	1 cut(s)	210
Eam1104I	CTCTTC	3 cut(s)	630, 714, 1751
EarI	CTCTTC	3 cut(s)	630, 714, 1751
Eco130I	CCWWGG	3 cut(s)	1121, 1399, 1451
Eco32I	GATATC	1 cut(s)	1156
Eco47I	GGWCC	2 cut(s)	274, 1645
Eco88I	CYCGRG	1 cut(s)	1420
EcoRI	GAATTC	1 cut(s)	614
EcoRII	CCWGG	1 cut(s)	525
EcoRV	GATATC	1 cut(s)	1156
EcoT14I	CCWWGG	3 cut(s)	1121, 1399, 1451
EcoT22I	ATGCAT	1 cut(s)	1281
ErhI	CCWWGG	3 cut(s)	1121, 1399, 1451
FaeI	CATG	7 cut(s)	161, 284, 883, 895, 1009, 1125, 1283
FalI	AAGNNNNNCTT	2 cut(s)	694, 726
FaqI	GGGAC	2 cut(s)	156, 1437
FatI	CATG	7 cut(s)	157, 280, 879, 891, 1005, 1121, 1279
FauNDI	CATATG	1 cut(s)	1489
FbaI	TGATCA	2 cut(s)	841, 882
FblI	GTMKAC	1 cut(s)	1566
Fnu4HI	GCNGC	2 cut(s)	1344, 1602
FokI	GGATG	6 cut(s)	113, 302, 494, 1416, 1423, 1488
Fsp4HI	GCNGC	2 cut(s)	1344, 1602
FspBI	CTAG	1 cut(s)	1148
FspI	TGCGCA	1 cut(s)	1640
GlaI	GCGC	2 cut(s)	1375, 1640
GluI	GCNGC	2 cut(s)	1344, 1602
GsaI	CCCAGC	1 cut(s)	29
GsuI	CTGGAG	2 cut(s)	699, 1491
HaeIII	GGCC	3 cut(s)	156, 942, 1096
HapII	CCGG	4 cut(s)	108, 1408, 1421, 1505
HhaI	GCGC	2 cut(s)	1376, 1641
Hin1II	CATG	7 cut(s)	161, 284, 883, 895, 1009, 1125, 1283
Hin6I	GCGC	2 cut(s)	1374, 1639
HinP1I	GCGC	2 cut(s)	1374, 1639
HindIII	AAGCTT	2 cut(s)	61, 911
HinfI	GANTC	8 cut(s)	235, 516, 522, 650, 722, 920, 1012, 1745
HpaII	CCGG	4 cut(s)	108, 1408, 1421, 1505
HphI	GGTGA	4 cut(s)	23, 122, 653, 978
Hpy166II	GTNNAC	8 cut(s)	199, 230, 661, 830, 897, 1016, 1261, 1567
Hpy188I	TCNGA	8 cut(s)	121, 521, 538, 786, 874, 919, 999, 1549
Hpy8I	GTNNAC	8 cut(s)	199, 230, 661, 830, 897, 1016, 1261, 1567
HpyAV	CCTTC	6 cut(s)	8, 472, 604, 1067, 1333, 1523
HpyCH4III	ACNGT	5 cut(s)	190, 665, 762, 1428, 1530
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1289
HpyF3I	CTNAG	4 cut(s)	69, 348, 474, 783
Hsp92II	CATG	7 cut(s)	161, 284, 883, 895, 1009, 1125, 1283
HspAI	GCGC	2 cut(s)	1374, 1639
Kpn2I	TCCGGA	1 cut(s)	1504
Ksp22I	TGATCA	2 cut(s)	841, 882
Kzo9I	GATC	8 cut(s)	262, 841, 882, 994, 1210, 1549, 1591, 1725
Lsp1109I	GCAGC	2 cut(s)	1330, 1588
LweI	GCATC	5 cut(s)	125, 333, 829, 1394, 1806
MaeI	CTAG	1 cut(s)	1148
MaeIII	GTNAC	7 cut(s)	160, 591, 930, 1001, 1182, 1361, 1440
MalI	GATC	8 cut(s)	264, 843, 884, 996, 1212, 1551, 1593, 1727
MbiI	CCGCTC	1 cut(s)	6
MboI	GATC	8 cut(s)	262, 841, 882, 994, 1210, 1549, 1591, 1725
MfeI	CAATTG	2 cut(s)	624, 1494
MflI	RGATCY	2 cut(s)	1210, 1591
MhlI	GDGCHC	1 cut(s)	1164
MlyI	GAGTC	2 cut(s)	731, 1021
MmeI	TCCRAC	2 cut(s)	1203, 1753
Mph1103I	ATGCAT	1 cut(s)	1281
MroI	TCCGGA	1 cut(s)	1504
MroXI	GAANNNNTTC	3 cut(s)	614, 1512, 1543
MseI	TTAA	8 cut(s)	32, 209, 369, 701, 803, 1080, 1254, 1835
MspCI	CTTAAG	1 cut(s)	700
MspI	CCGG	4 cut(s)	108, 1408, 1421, 1505
MspR9I	CCNGG	3 cut(s)	527, 1421, 1422
MunI	CAATTG	2 cut(s)	624, 1494
Mva1269I	GAATGC	1 cut(s)	695
MvaI	CCWGG	1 cut(s)	527
MwoI	GCNNNNNNNGC	1 cut(s)	1289
NciI	CCSGG	2 cut(s)	1421, 1422
NcoI	CCATGG	1 cut(s)	1121
NdeI	CATATG	1 cut(s)	1489
NdeII	GATC	8 cut(s)	262, 841, 882, 994, 1210, 1549, 1591, 1725
NlaIII	CATG	7 cut(s)	161, 284, 883, 895, 1009, 1125, 1283
NlaIV	GGNNCC	3 cut(s)	106, 1302, 1509
NmuCI	GTSAC	5 cut(s)	591, 930, 1001, 1182, 1361
NsbI	TGCGCA	1 cut(s)	1640
NsiI	ATGCAT	1 cut(s)	1281
NspI	RCATGY	3 cut(s)	895, 1009, 1283
PaeI	GCATGC	1 cut(s)	1283
PciI	ACATGT	1 cut(s)	1005
PctI	GAATGC	1 cut(s)	695
PdmI	GAANNNNTTC	3 cut(s)	614, 1512, 1543
PfeI	GAWTC	6 cut(s)	235, 516, 522, 650, 920, 1745
PfoI	TCCNGGA	1 cut(s)	525
PkrI	GCNGC	2 cut(s)	1345, 1603
PleI	GAGTC	2 cut(s)	730, 1020
PpsI	GAGTC	2 cut(s)	730, 1020
PscI	ACATGT	1 cut(s)	1005
PshBI	ATTAAT	1 cut(s)	1254
PsiI	TTATAA	1 cut(s)	1350
Psp6I	CCWGG	1 cut(s)	525
PspFI	CCCAGC	1 cut(s)	25
PspGI	CCWGG	1 cut(s)	525
PspN4I	GGNNCC	3 cut(s)	106, 1302, 1509
PspPI	GGNCC	4 cut(s)	154, 274, 1094, 1645
PsrI	GAACNNNNNNTAC	2 cut(s)	1500, 1532
PstNI	CAGNNNCTG	1 cut(s)	1594
PsuI	RGATCY	2 cut(s)	1210, 1591
RsaI	GTAC	6 cut(s)	198, 229, 829, 1047, 1260, 1616
RsaNI	GTAC	6 cut(s)	197, 228, 828, 1046, 1259, 1615
SaqAI	TTAA	8 cut(s)	32, 209, 369, 701, 803, 1080, 1254, 1835
SatI	GCNGC	2 cut(s)	1344, 1602
Sau3AI	GATC	8 cut(s)	262, 841, 882, 994, 1210, 1549, 1591, 1725
Sau96I	GGNCC	4 cut(s)	154, 274, 1094, 1645
SchI	GAGTC	2 cut(s)	731, 1021
ScrFI	CCNGG	3 cut(s)	527, 1421, 1422
SduI	GDGCHC	1 cut(s)	1164
SfaNI	GCATC	5 cut(s)	125, 333, 829, 1394, 1806
SfcI	CTRYAG	2 cut(s)	239, 1192
SinI	GGWCC	2 cut(s)	274, 1645
SmaI	CCCGGG	1 cut(s)	1422
SmlI	CTYRAG	2 cut(s)	245, 700
SmoI	CTYRAG	2 cut(s)	245, 700
SphI	GCATGC	1 cut(s)	1283
SsiI	CCGC	3 cut(s)	6, 1556, 1789
SspMI	CTAG	1 cut(s)	1148
StyD4I	CCNGG	3 cut(s)	525, 1419, 1420
StyI	CCWWGG	3 cut(s)	1121, 1399, 1451
TaaI	ACNGT	5 cut(s)	190, 665, 762, 1428, 1530
TaqI	TCGA	3 cut(s)	1158, 1621, 1800
TatI	WGTACW	4 cut(s)	196, 227, 1258, 1614
TfiI	GAWTC	6 cut(s)	235, 516, 522, 650, 920, 1745
Tru1I	TTAA	8 cut(s)	32, 209, 369, 701, 803, 1080, 1254, 1835
Tru9I	TTAA	8 cut(s)	32, 209, 369, 701, 803, 1080, 1254, 1835
TscAI	CASTG	4 cut(s)	589, 661, 1433, 1533
TseFI	GTSAC	5 cut(s)	591, 930, 1001, 1182, 1361
TseI	GCWGC	2 cut(s)	1343, 1601
Tsp45I	GTSAC	5 cut(s)	591, 930, 1001, 1182, 1361
TspDTI	ATGAA	6 cut(s)	498, 936, 948, 1023, 1055, 1491
TspGWI	ACGGA	1 cut(s)	266
TspMI	CCCGGG	1 cut(s)	1420
TspRI	CASTG	4 cut(s)	589, 661, 1433, 1533
Vha464I	CTTAAG	1 cut(s)	700
VpaK11BI	GGWCC	2 cut(s)	274, 1645
VspI	ATTAAT	1 cut(s)	1254
XapI	RAATTY	5 cut(s)	452, 601, 614, 799, 1653
XceI	RCATGY	3 cut(s)	895, 1009, 1283
XmaI	CCCGGG	1 cut(s)	1420
XmiI	GTMKAC	1 cut(s)	1566
XmnI	GAANNNNTTC	3 cut(s)	614, 1512, 1543
XspI	CTAG	1 cut(s)	1148
Zsp2I	ATGCAT	1 cut(s)	1281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.