Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009071

Physical Location & Seq

Genomic Coordinates Forward (+)

41802202 .. 41806760

Gene Structure 4559 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1351400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 894 bp

ATGGCTTCGGCCGCTTCGAAGCTCTACGCCGACGATGTGAGCCTTCTGATGGTTTTGCTGGACACGAACCCTTTCTTCTGGAGCTCATCCAATCTCCCGTTCTCCAAGTTTCTCCCCCACGTACTTGCGTTTCTGAACTCGATTCTGCTGCTCAATCAACTCAACCAGGTGGTGGTTATTACCACGGGGTACAATTCCTGCAGCTACATCTACGATTCTTCTTCGGACTCGAACCAGGGCTCTGATCATGGAAGAATAATGCCGGCGCGGTGCTCTAACTTGTTGCAGAAGCTGGAGGAATTCGTGATCAAAGACGAGCAATTGTTCAAAGAAGGGTCGAGAGAAGGGATTTCTTCTTCTCTGCTATCTGGGTCTTTATCAATGGCTCTTTGTTATATACAGAGGGTTTTTCGATCAGGGCCACTTCATCCTCAACCTCGCATTTTATGCTTGCAGGGATCATCGGATGGACCTGAACAATATGTTGCAATCATGAATTCAATATTTTCTGCACAGCGTTCTATGGTTCCTATAGATTCTTGCTATATGGGATCGAGCAACTCTGCCTTCCTACAGCAGGCTTCATATATTACTGGTGGTGTGTATCTGAAGCCCCAACAACCAAATGGACTGTTTCAGTATCTCTCGACAGTTTTTGCAACTGATTTGCATTCTCGAGCATTTTTACAGCTTCCTAAGTCTCTCGGTGTGGATTTTCGTGCCTCGTGTTTTTGCCATAAGAAGACAATAGACATGGGCTACATTTGTTCTGTGTGCTTGTCCATTTTTTGTAAGCATCACAAGAAATGTTCAACTTGTGGGTCAGTATTTGGTCAAGCCCAGTTAGATGCTTCCTCAACATCCAACAGGAAAAGAAAGACGCCAGAGACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000439 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005675 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006355 GO:0006357 GO:0006412 GO:0006464 GO:0006468 GO:0006518 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016310 GO:0016591 GO:0019219 GO:0019222 GO:0019538 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032806 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070816 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1902554 GO:1902911 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 ko05203 map03022 map03420 map05203

Pfam Domains

Protein Families

Tfb4

Protein Analysis

298

Amino Acids

32.77

Weight (kDa)

8.56

Isoelectric Point (pI)

45.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tfb4	PF03850	13 - 274	2.4e-95	Transcription factor Tfb4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009071)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18340
fragaria_vesca	FvH4_2g26720 FvH4_2g26720 FvH4_2g26720 FvH4_2g26720 FvH4_2g26720 FvH4_2g26720 FvH4_2g26720
malus_domestica	MD15G1351400.v1.1
prunus_persica	Prupe.1G500100_v2.0.a1 Prupe.1G500100_v2.0.a1 Prupe.1G500100_v2.0.a1
pyrus_communis	pycom15g31380
rosa_chinensis	RchiOBHm_Chr6g0295621
rosa_laevigata	RLG00000011784
rosa_multiflora	Rmu_sc0013988.1_g000005
rosa_roxburghii	Rroxscaffold_7G00172310
rosa_rugosa	Rorug06G0252900
rosa_samantha	Rh6BG374000 Rh6CG380300 Rh6DG366900
rosa_wichuraiana	Rw6G031930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	172
AccII	CGCG	1 cut(s)	268
AciI	CCGC	2 cut(s)	12, 268
AclWI	GGATC	2 cut(s)	466, 559
AcoI	YGGCCR	1 cut(s)	9
AcsI	RAATTY	2 cut(s)	299, 496
AcuI	CTGAAG	1 cut(s)	629
AcyI	GRCGYC	1 cut(s)	881
AfaI	GTAC	2 cut(s)	123, 191
AfiI	CCNNNNNNNGG	3 cut(s)	49, 172, 577
AgsI	TTSAA	3 cut(s)	328, 501, 813
AjnI	CCWGG	2 cut(s)	165, 234
AjuI	GAANNNNNNNTTGG	2 cut(s)	83, 115
AluBI	AGCT	5 cut(s)	22, 84, 204, 292, 691
AluI	AGCT	5 cut(s)	22, 84, 204, 292, 691
Alw21I	GWGCWC	2 cut(s)	86, 275
Alw26I	GTCTC	2 cut(s)	705, 881
AlwI	GGATC	2 cut(s)	466, 559
AlwNI	CAGNNNCTG	1 cut(s)	292
Ama87I	CYCGRG	1 cut(s)	675
AoxI	GGCC	2 cut(s)	9, 419
ApeKI	GCWGC	2 cut(s)	148, 201
ApoI	RAATTY	2 cut(s)	299, 496
Asp700I	GAANNNNTTC	1 cut(s)	71
AspLEI	GCGC	1 cut(s)	268
AspS9I	GGNCC	2 cut(s)	419, 470
AsuII	TTCGAA	1 cut(s)	17
AvaI	CYCGRG	1 cut(s)	675
AvaII	GGWCC	1 cut(s)	470
BanII	GRGCYC	2 cut(s)	86, 242
BauI	CACGAG	1 cut(s)	724
BbsI	GAAGAC	1 cut(s)	749
Bbv12I	GWGCWC	2 cut(s)	86, 275
BbvI	GCAGC	2 cut(s)	135, 213
BccI	CCATC	2 cut(s)	43, 461
BcgI	CGANNNNNNTGC	2 cut(s)	130, 164
BciT130I	CCWGG	2 cut(s)	167, 236
BclI	TGATCA	2 cut(s)	244, 306
BcoDI	GTCTC	2 cut(s)	705, 881
BfmI	CTRYAG	3 cut(s)	199, 531, 572
BisI	GCNGC	3 cut(s)	12, 149, 202
BlsI	GCNGC	3 cut(s)	13, 150, 203
Bme1390I	CCNGG	2 cut(s)	167, 236
Bme18I	GGWCC	1 cut(s)	470
BmeT110I	CYCGRG	1 cut(s)	675
BmgT120I	GGNCC	2 cut(s)	419, 470
BmiI	GGNNCC	1 cut(s)	528
BmrFI	CCNGG	2 cut(s)	167, 236
BmrI	ACTGGG	1 cut(s)	835
BmsI	GCATC	2 cut(s)	805, 838
BmuI	ACTGGG	1 cut(s)	835
BpiI	GAAGAC	1 cut(s)	749
BpmI	CTGGAG	2 cut(s)	100, 314
Bpu14I	TTCGAA	1 cut(s)	17
BsaAI	YACGTR	1 cut(s)	121
BsaHI	GRCGYC	1 cut(s)	881
BsaJI	CCNNGG	2 cut(s)	183, 235
Bsc4I	CCNNNNNNNGG	3 cut(s)	49, 172, 577
Bse118I	RCCGGY	1 cut(s)	262
Bse1I	ACTGG	2 cut(s)	598, 841
BseBI	CCWGG	2 cut(s)	167, 236
BseDI	CCNNGG	2 cut(s)	183, 235
BseGI	GGATG	4 cut(s)	86, 427, 472, 860
BseLI	CCNNNNNNNGG	3 cut(s)	49, 172, 577
BseNI	ACTGG	2 cut(s)	598, 841
BseX3I	CGGCCG	1 cut(s)	9
BseXI	GCAGC	2 cut(s)	135, 213
BsgI	GTGCAG	1 cut(s)	495
Bsh1236I	CGCG	1 cut(s)	268
Bsh1285I	CGRYCG	1 cut(s)	12
BshFI	GGCC	2 cut(s)	11, 421
BsiEI	CGRYCG	1 cut(s)	12
BsiHKAI	GWGCWC	2 cut(s)	86, 275
BsiHKCI	CYCGRG	1 cut(s)	675
BsiSI	CCGG	1 cut(s)	263
BslI	CCNNNNNNNGG	3 cut(s)	49, 172, 577
BsmAI	GTCTC	2 cut(s)	705, 881
BsmI	GAATGC	1 cut(s)	670
BsnI	GGCC	2 cut(s)	11, 421
BsoBI	CYCGRG	1 cut(s)	675
Bsp119I	TTCGAA	1 cut(s)	17
Bsp1286I	GDGCHC	3 cut(s)	86, 242, 275
Bsp143I	GATC	5 cut(s)	244, 306, 413, 458, 551
BspACI	CCGC	2 cut(s)	12, 268
BspANI	GGCC	2 cut(s)	11, 421
BspFNI	CGCG	1 cut(s)	268
BspHI	TCATGA	1 cut(s)	492
BspLI	GGNNCC	1 cut(s)	528
BspMAI	CTGCAG	1 cut(s)	203
BspPI	GGATC	2 cut(s)	466, 559
BspT104I	TTCGAA	1 cut(s)	17
BsrFI	RCCGGY	1 cut(s)	262
BsrI	ACTGG	2 cut(s)	598, 841
BssAI	RCCGGY	1 cut(s)	262
BssECI	CCNNGG	2 cut(s)	183, 235
BssMI	GATC	5 cut(s)	244, 306, 413, 458, 551
BssNI	GRCGYC	1 cut(s)	881
BssSI	CACGAG	1 cut(s)	724
Bst2BI	CACGAG	1 cut(s)	724
Bst2UI	CCWGG	2 cut(s)	167, 236
Bst4CI	ACNGT	2 cut(s)	633, 652
BstACI	GRCGYC	1 cut(s)	881
BstAPI	GCANNNNNTGC	1 cut(s)	447
BstBAI	YACGTR	1 cut(s)	121
BstBI	TTCGAA	1 cut(s)	17
BstC8I	GCNNGC	3 cut(s)	264, 452, 579
BstDEI	CTNAG	1 cut(s)	696
BstDSI	CCRYGG	1 cut(s)	183
BstENI	CCTNNNNNAGG	1 cut(s)	575
BstF5I	GGATG	4 cut(s)	86, 427, 472, 860
BstFNI	CGCG	1 cut(s)	268
BstHHI	GCGC	1 cut(s)	268
BstKTI	GATC	5 cut(s)	247, 309, 416, 461, 554
BstMAI	GTCTC	2 cut(s)	705, 881
BstMBI	GATC	5 cut(s)	244, 306, 413, 458, 551
BstMCI	CGRYCG	1 cut(s)	12
BstMWI	GCNNNNNNNGC	2 cut(s)	11, 447
BstNI	CCWGG	2 cut(s)	167, 236
BstSCI	CCNGG	2 cut(s)	165, 234
BstSFI	CTRYAG	3 cut(s)	199, 531, 572
BstUI	CGCG	1 cut(s)	268
BstV1I	GCAGC	2 cut(s)	135, 213
BstV2I	GAAGAC	1 cut(s)	749
BstZI	CGGCCG	1 cut(s)	9
BsuRI	GGCC	2 cut(s)	11, 421
BtgI	CCRYGG	1 cut(s)	183
BtsCI	GGATG	4 cut(s)	86, 427, 472, 860
Cac8I	GCNNGC	3 cut(s)	264, 452, 579
CaiI	CAGNNNCTG	1 cut(s)	292
CciI	TCATGA	1 cut(s)	492
CfoI	GCGC	1 cut(s)	268
Cfr10I	RCCGGY	1 cut(s)	262
Cfr13I	GGNCC	2 cut(s)	419, 470
CseI	GACGC	1 cut(s)	889
CsiI	ACCWGGT	1 cut(s)	165
Csp6I	GTAC	2 cut(s)	122, 190
CviAII	CATG	3 cut(s)	248, 493, 754
CviQI	GTAC	2 cut(s)	122, 190
DdeI	CTNAG	1 cut(s)	696
DpnI	GATC	5 cut(s)	246, 308, 415, 460, 553
DpnII	GATC	5 cut(s)	244, 306, 413, 458, 551
EaeI	YGGCCR	1 cut(s)	9
EagI	CGGCCG	1 cut(s)	9
Ecl136II	GAGCTC	1 cut(s)	84
EclXI	CGGCCG	1 cut(s)	9
Eco24I	GRGCYC	2 cut(s)	86, 242
Eco47I	GGWCC	1 cut(s)	470
Eco52I	CGGCCG	1 cut(s)	9
Eco53kI	GAGCTC	1 cut(s)	84
Eco57I	CTGAAG	1 cut(s)	629
Eco88I	CYCGRG	1 cut(s)	675
EcoICRI	GAGCTC	1 cut(s)	84
EcoNI	CCTNNNNNAGG	1 cut(s)	575
EcoRI	GAATTC	2 cut(s)	299, 496
EcoRII	CCWGG	2 cut(s)	165, 234
EcoT38I	GRGCYC	2 cut(s)	86, 242
FaeI	CATG	3 cut(s)	251, 496, 757
FatI	CATG	3 cut(s)	247, 492, 753
FbaI	TGATCA	2 cut(s)	244, 306
Fnu4HI	GCNGC	3 cut(s)	12, 149, 202
FokI	GGATG	4 cut(s)	73, 414, 479, 847
FriOI	GRGCYC	2 cut(s)	86, 242
Fsp4HI	GCNGC	3 cut(s)	12, 149, 202
GlaI	GCGC	1 cut(s)	267
GluI	GCNGC	3 cut(s)	12, 149, 202
GsuI	CTGGAG	2 cut(s)	100, 314
HaeIII	GGCC	2 cut(s)	11, 421
HapII	CCGG	1 cut(s)	263
HgaI	GACGC	1 cut(s)	889
HhaI	GCGC	1 cut(s)	268
Hin1I	GRCGYC	1 cut(s)	881
Hin1II	CATG	3 cut(s)	251, 496, 757
Hin6I	GCGC	1 cut(s)	266
HinP1I	GCGC	1 cut(s)	266
HinfI	GANTC	4 cut(s)	142, 215, 227, 536
HpaII	CCGG	1 cut(s)	263
Hpy188I	TCNGA	6 cut(s)	48, 135, 226, 244, 466, 609
Hpy188III	TCNNGA	6 cut(s)	79, 304, 339, 493, 646, 675
Hpy99I	CGWCG	1 cut(s)	35
HpyAV	CCTTC	4 cut(s)	53, 326, 338, 577
HpyCH4III	ACNGT	2 cut(s)	633, 652
HpyCH4IV	ACGT	1 cut(s)	120
HpyCH4V	TGCA	7 cut(s)	201, 286, 454, 488, 512, 659, 670
HpyF10VI	GCNNNNNNNGC	2 cut(s)	11, 447
HpyF3I	CTNAG	1 cut(s)	696
HpySE526I	ACGT	1 cut(s)	120
Hsp92I	GRCGYC	1 cut(s)	881
Hsp92II	CATG	3 cut(s)	251, 496, 757
HspAI	GCGC	1 cut(s)	266
KroI	GCCGGC	1 cut(s)	262
KroNI	GCCGGC	1 cut(s)	264
Ksp22I	TGATCA	2 cut(s)	244, 306
Kzo9I	GATC	5 cut(s)	244, 306, 413, 458, 551
LmnI	GCTCC	1 cut(s)	81
Lsp1109I	GCAGC	2 cut(s)	135, 213
LweI	GCATC	2 cut(s)	805, 838
MabI	ACCWGGT	1 cut(s)	165
MaeII	ACGT	1 cut(s)	120
MalI	GATC	5 cut(s)	246, 308, 415, 460, 553
MboI	GATC	5 cut(s)	244, 306, 413, 458, 551
MboII	GAAGA	7 cut(s)	67, 210, 213, 264, 345, 348, 754
MfeI	CAATTG	1 cut(s)	320
MhlI	GDGCHC	3 cut(s)	86, 242, 275
MluCI	AATT	4 cut(s)	193, 299, 320, 496
MlyI	GAGTC	1 cut(s)	221
MmeI	TCCRAC	1 cut(s)	888
MnlI	CCTC	6 cut(s)	289, 396, 441, 447, 733, 865
MroNI	GCCGGC	1 cut(s)	262
MroXI	GAANNNNTTC	1 cut(s)	71
MspI	CCGG	1 cut(s)	263
MspR9I	CCNGG	2 cut(s)	167, 236
MunI	CAATTG	1 cut(s)	320
Mva1269I	GAATGC	1 cut(s)	670
MvaI	CCWGG	2 cut(s)	167, 236
MvnI	CGCG	1 cut(s)	268
MwoI	GCNNNNNNNGC	2 cut(s)	11, 447
NaeI	GCCGGC	1 cut(s)	264
NdeII	GATC	5 cut(s)	244, 306, 413, 458, 551
NgoMIV	GCCGGC	1 cut(s)	262
NlaIII	CATG	3 cut(s)	251, 496, 757
NlaIV	GGNNCC	1 cut(s)	528
NspV	TTCGAA	1 cut(s)	17
PaeR7I	CTCGAG	1 cut(s)	675
PagI	TCATGA	1 cut(s)	492
PcsI	WCGNNNNNNNCGW	1 cut(s)	14
PctI	GAATGC	1 cut(s)	670
PdiI	GCCGGC	1 cut(s)	264
PdmI	GAANNNNTTC	1 cut(s)	71
PfeI	GAWTC	3 cut(s)	142, 215, 536
PflMI	CCANNNNNTGG	1 cut(s)	172
PkrI	GCNGC	3 cut(s)	13, 150, 203
PleI	GAGTC	1 cut(s)	221
PpsI	GAGTC	1 cut(s)	221
Ppu21I	YACGTR	1 cut(s)	121
Psp124BI	GAGCTC	1 cut(s)	86
Psp6I	CCWGG	2 cut(s)	165, 234
PspGI	CCWGG	2 cut(s)	165, 234
PspN4I	GGNNCC	1 cut(s)	528
PspPI	GGNCC	2 cut(s)	419, 470
PstI	CTGCAG	1 cut(s)	203
PstNI	CAGNNNCTG	1 cut(s)	292
RsaI	GTAC	2 cut(s)	123, 191
RsaNI	GTAC	2 cut(s)	122, 190
SacI	GAGCTC	1 cut(s)	86
SatI	GCNGC	3 cut(s)	12, 149, 202
Sau3AI	GATC	5 cut(s)	244, 306, 413, 458, 551
Sau96I	GGNCC	2 cut(s)	419, 470
SchI	GAGTC	1 cut(s)	221
ScrFI	CCNGG	2 cut(s)	167, 236
SduI	GDGCHC	3 cut(s)	86, 242, 275
SetI	ASST	9 cut(s)	24, 86, 123, 171, 206, 294, 439, 475, 693
SexAI	ACCWGGT	1 cut(s)	165
SfaNI	GCATC	2 cut(s)	805, 838
SfcI	CTRYAG	3 cut(s)	199, 531, 572
Sfr274I	CTCGAG	1 cut(s)	675
SfuI	TTCGAA	1 cut(s)	17
SinI	GGWCC	1 cut(s)	470
SlaI	CTCGAG	1 cut(s)	675
SmlI	CTYRAG	1 cut(s)	675
SmoI	CTYRAG	1 cut(s)	675
Sse9I	AATT	4 cut(s)	193, 299, 320, 496
SsiI	CCGC	2 cut(s)	12, 268
SspI	AATATT	1 cut(s)	504
SstI	GAGCTC	1 cut(s)	86
StyD4I	CCNGG	2 cut(s)	165, 234
TaaI	ACNGT	2 cut(s)	633, 652
TaiI	ACGT	1 cut(s)	123
TaqI	TCGA	8 cut(s)	17, 140, 230, 338, 412, 554, 647, 676
TasI	AATT	4 cut(s)	193, 299, 320, 496
TauI	GCSGC	1 cut(s)	14
TfiI	GAWTC	3 cut(s)	142, 215, 536
TseI	GCWGC	2 cut(s)	148, 201
TspDTI	ATGAA	3 cut(s)	416, 509, 573
Van91I	CCANNNNNTGG	1 cut(s)	172
VpaK11BI	GGWCC	1 cut(s)	470
XagI	CCTNNNNNAGG	1 cut(s)	575
XapI	RAATTY	2 cut(s)	299, 496
XcmI	CCANNNNNNNNNTGG	1 cut(s)	623
XhoI	CTCGAG	1 cut(s)	675
XmnI	GAANNNNTTC	1 cut(s)	71

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.