MD15G1390900.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016702
Physical Location & Seq
Forward (+)
48522974 .. 48528072
5099 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1390900.v1.1.491

Sequence Viewer

Length: 1842 bp
ATGCAATGTAAAACAGTAAGAGTAGCAATGGTTTTGCATGAACTGGCCCAAAAGCCCCCTCACAGACTCCTTGCAGAAAAATTCATCGCACTCTTGCAGTCCTGTAAGGCCATGAAGCAACTTCACCAAATTCAGACCCAGATAATCGCCCACGGTTTCTCACACAACGAGTACATTGCCCCGAAAATAGTCTCAGCATGCGCCGAACTGAAGCAAATGGCGTATGCCCACAAGGTGTTTGATGAAATACCTGATCCAAATGTTGCTCTATGGAACGCAATGTTCAGAGGGTATGGGAAGAATGAGAGTCATAGAGAAGTTATAGTATTGTTTAGTAGAATGAAGAGCATGGATATATTGCCCAATTGCTTCACATTCCCTGTCGTGATTAAATCTTGCGGAAAAATGAAACGGCTGGTAGAAGGTGAGATGGTGCATTGTGTAGTGATAAAATGTGGGTTTGGAGCAAACCCATATGTGGGAACGACACTGATTGAAATGTATGCAGCTGGGGGACTGATTGGAGCTGCTTACAAGGTGTTTGGTGAGATGTTTGAGAGGAATGTGGTTGCGTGGACTTCCATGATTAATGGGTACATTTTGTGTGGTGATATGGTTTCTGCTCAGCGCCTTTTTGATTTGGCTCCGGAACGTGATATTGTGTTGTGGAACACTATGATTTCAGGTTACATTGAGCTGAGTGATATGGTGGCGGCTAGAAAACTTTTTGATGAAATGCCAAGACAAGATGTTATGTGTTGGAATACGGTTTTGAATGGTTATGCAAGTAATGGGGACATTGAGGCTTGTGAGAGGTTGTTTGAGGAGATGCCTGAGAGGAATGTTTTCTCTTGGAATGGGTTGATTGGAGGGTATGCGCGTAATGGACGGTTCTTTGAAACTCTGGGATCTTTCAAGCGGATGCTAAGTGAGAGTGATTTGCTTCCTAATGATGCCACCTTAGTGACTGTTTTGTCAGCCTGTGCAAGACTAGGAGCTCTTGATTTGGGTAAGTGGCTACATGTATACGCAGAGAGCATTGGGTACAAAAGAAATGTCTATGTTGGGAATGCTTTAATTGACATGTATGCAAAATGTGGGATTATTGATAACGCACTAGATGTGTTCATGAGCATGGATAAGAAAGATTTAATCAGTTGGAACACAATCATTTGTGGCTTGGCAATGCACGGTCGTGGAGCTGATGCCTTAAATTTGTTTAGCCAGATGATGAATTGTGGAGGAAAGCCAGATGCCATCACCTTCGTAGGCATTTTGTGCTCTTGTACGCATCTTGGATTAGTCGAAGATGGCCTTTTGTATTTCCAGTCAATGGTTGATGACTATTCCATTGTGCCTCAGATTGAGCATTATGGCTGTATGGTTGATCTGCTTGGACGCTCTGGTCTTTTGGACCAGGCTATGAACTTTGTGAGGAAGATGCCAATGGAAGCAGATGCTGTTATTTGGGCTGCCTTACTTGGTGCATGTCGGATTTACAAGAAAATTGACTTGGCAGAGTTGGCACTTGAAAGGCTAATCGAACTCGAACCAAAAAACCCTGCAAATTATGTCATGCTTTCAAACATATATGGTGATCTTGGGAGGTGGAAAGATGTTGCAAGACTAAAAGTTTCAATGAGGGATACAGGACATAAAAAATTCCCTGGTGTTAGCCTTATAGAGGTGAATGATGGTGTGGTTGAGTTTTATTCCTTGGATAAGAGACATCCTGAGACTGAGGAAATATATGGTGCCTTGATGGGGTTGACGAAACTGCTAAGATCATCTGGATATGTACCAAACATTCTGGAGCTTGGGCAGGGAGCCTATCACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

614

Amino Acids

68.6

Weight (kDa)

6.05

Isoelectric Point (pI)

42.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 86 - 135 1.2e-09 PPR repeat family
PPR_2 PF13041 218 - 249 2.2e-06 PPR repeat family
PPR_2 PF13041 250 - 282 1.5e-07 PPR repeat family
PPR PF01535 253 - 282 2e-07 PPR repeat
PPR_2 PF13041 383 - 429 5.3e-09 PPR repeat family
E_motif PF20431 500 - 562 1.8e-22 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13410
fragaria_vesca FvH4_4g27250 FvH4_4g27250 FvH4_4g27250
malus_domestica MD15G1390900.v1.1
prunus_persica Prupe.1G312300_v2.0.a1
pyrus_communis pycom15g35000
rosa_chinensis RchiOBHm_Chr4g0435031
rosa_laevigata RLG00000006626
rosa_multiflora Rmu_sc0000400.1_g000012
rosa_roxburghii Rroxscaffold_5G00376240
rosa_rugosa Rorug04G0281900
rosa_samantha Rh4BG345300
rosa_wichuraiana Rw4G029410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 973, 1404
AccB1I GGYRCC 1 cut(s) 1754
AccB7I CCANNNNNTGG 1 cut(s) 1333
AccI GTMKAC 1 cut(s) 1026
AccII CGCG 1 cut(s) 880
AccIII TCCGGA 1 cut(s) 646
AciI CCGC 3 cut(s) 399, 713, 919
AclWI GGATC 2 cut(s) 248, 916
AcsI RAATTY 4 cut(s) 80, 129, 1213, 1661
AcuI CTGAAG 1 cut(s) 230
AdeI CACNNNGTG 1 cut(s) 235
AfaI GTAC 5 cut(s) 173, 596, 1046, 1288, 1800
AfiI CCNNNNNNNGG 5 cut(s) 478, 479, 1333, 1684, 1764
AflIII ACRYGT 2 cut(s) 1021, 1083
AgsI TTSAA 7 cut(s) 497, 775, 899, 916, 1532, 1584, 1638
AjnI CCWGG 2 cut(s) 1416, 1666
AjuI GAANNNNNNNTTGG 2 cut(s) 1496, 1528
AleI CACNNNNGTG 2 cut(s) 962, 1194
AluBI AGCT 6 cut(s) 509, 527, 697, 998, 1202, 1816
AluI AGCT 6 cut(s) 509, 527, 697, 998, 1202, 1816
Alw21I GWGCWC 2 cut(s) 1000, 1283
Alw26I GTCTC 3 cut(s) 196, 1720, 1730
AlwI GGATC 2 cut(s) 248, 916
AlwNI CAGNNNCTG 1 cut(s) 1460
Aor13HI TCCGGA 1 cut(s) 646
AoxI GGCC 3 cut(s) 45, 108, 1312
ApeKI GCWGC 3 cut(s) 506, 527, 1472
ApoI RAATTY 4 cut(s) 80, 129, 1213, 1661
ArsI GACNNNNNNTTYG 2 cut(s) 1041, 1073
AseI ATTAAT 1 cut(s) 588
Asp700I GAANNNNTTC 1 cut(s) 845
AspLEI GCGC 3 cut(s) 203, 630, 880
AspS9I GGNCC 2 cut(s) 46, 1414
AsuHPI GGTGA 7 cut(s) 116, 437, 557, 620, 1252, 1607, 1699
AvaII GGWCC 1 cut(s) 1414
BanI GGYRCC 1 cut(s) 1754
BanII GRGCYC 1 cut(s) 1000
Bbv12I GWGCWC 2 cut(s) 1000, 1283
BbvI GCAGC 3 cut(s) 514, 518, 1459
BccI CCATC 5 cut(s) 424, 1265, 1304, 1688, 1756
BceAI ACGGC 1 cut(s) 428
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 2 cut(s) 1418, 1668
BciVI GTATCC 1 cut(s) 1639
BcoDI GTCTC 3 cut(s) 196, 1720, 1730
BfaI CTAG 3 cut(s) 717, 992, 1118
BfoI RGCGCY 1 cut(s) 631
BfuI GTATCC 1 cut(s) 1639
BisI GCNGC 4 cut(s) 507, 528, 714, 1473
BlpI GCTNAGC 1 cut(s) 624
BlsI GCNGC 4 cut(s) 508, 529, 715, 1474
Bme1390I CCNGG 2 cut(s) 1418, 1668
Bme18I GGWCC 1 cut(s) 1414
BmgT120I GGNCC 2 cut(s) 46, 1414
BmiI GGNNCC 3 cut(s) 645, 1756, 1828
BmrFI CCNGG 2 cut(s) 1418, 1668
BmsI GCATC 8 cut(s) 819, 912, 943, 1195, 1243, 1300, 1431, 1447
BpmI CTGGAG 1 cut(s) 1832
Bpu1102I GCTNAGC 1 cut(s) 624
BsaJI CCNNGG 3 cut(s) 151, 1666, 1716
BsaWI WCCGGW 1 cut(s) 646
Bsc4I CCNNNNNNNGG 5 cut(s) 478, 479, 1333, 1684, 1764
Bse1I ACTGG 2 cut(s) 48, 1327
Bse3DI GCAATG 5 cut(s) 11, 33, 174, 285, 1191
BseAI TCCGGA 1 cut(s) 646
BseBI CCWGG 2 cut(s) 1418, 1668
BseDI CCNNGG 3 cut(s) 151, 1666, 1716
BseGI GGATG 2 cut(s) 927, 1729
BseLI CCNNNNNNNGG 5 cut(s) 478, 479, 1333, 1684, 1764
BseMI GCAATG 5 cut(s) 11, 33, 174, 285, 1191
BseMII CTCAG 7 cut(s) 207, 638, 689, 825, 1373, 1725, 1731
BseNI ACTGG 2 cut(s) 48, 1327
BseRI GAGGAG 1 cut(s) 839
BseXI GCAGC 3 cut(s) 514, 518, 1459
BseYI CCCAGC 1 cut(s) 509
Bsh1236I CGCG 1 cut(s) 880
Bsh1285I CGRYCG 1 cut(s) 1195
BshFI GGCC 3 cut(s) 47, 110, 1314
BshNI GGYRCC 1 cut(s) 1754
BsiEI CGRYCG 1 cut(s) 1195
BsiHKAI GWGCWC 2 cut(s) 1000, 1283
BsiSI CCGG 1 cut(s) 647
BslFI GGGAC 2 cut(s) 528, 809
BslI CCNNNNNNNGG 5 cut(s) 478, 479, 1333, 1684, 1764
BsmAI GTCTC 3 cut(s) 196, 1720, 1730
BsmFI GGGAC 2 cut(s) 528, 809
BsmI GAATGC 1 cut(s) 1075
BsnI GGCC 3 cut(s) 47, 110, 1314
Bsp1286I GDGCHC 2 cut(s) 1000, 1283
Bsp13I TCCGGA 1 cut(s) 646
Bsp143I GATC 5 cut(s) 253, 908, 1387, 1597, 1784
Bsp1720I GCTNAGC 1 cut(s) 624
BspACI CCGC 3 cut(s) 399, 713, 919
BspANI GGCC 3 cut(s) 47, 110, 1314
BspCNI CTCAG 7 cut(s) 206, 637, 690, 826, 1372, 1726, 1732
BspEI TCCGGA 1 cut(s) 646
BspFNI CGCG 1 cut(s) 880
BspHI TCATGA 1 cut(s) 1128
BspLI GGNNCC 3 cut(s) 645, 1756, 1828
BspPI GGATC 2 cut(s) 248, 916
BspQI GCTCTTC 1 cut(s) 338
BspT107I GGYRCC 1 cut(s) 1754
BsrDI GCAATG 5 cut(s) 11, 33, 174, 285, 1191
BsrI ACTGG 2 cut(s) 48, 1327
BssECI CCNNGG 3 cut(s) 151, 1666, 1716
BssMI GATC 5 cut(s) 253, 908, 1387, 1597, 1784
BssNAI GTATAC 1 cut(s) 1027
BssT1I CCWWGG 1 cut(s) 1716
Bst1107I GTATAC 1 cut(s) 1027
Bst2UI CCWGG 2 cut(s) 1418, 1668
Bst4CI ACNGT 6 cut(s) 16, 155, 769, 891, 970, 1193
Bst6I CTCTTC 1 cut(s) 338
BstAPI GCANNNNNTGC 1 cut(s) 1278
BstC8I GCNNGC 1 cut(s) 199
BstDSI CCRYGG 1 cut(s) 151
BstENI CCTNNNNNAGG 1 cut(s) 1682
BstF5I GGATG 2 cut(s) 927, 1729
BstFNI CGCG 1 cut(s) 880
BstH2I RGCGCY 1 cut(s) 631
BstHHI GCGC 3 cut(s) 203, 630, 880
BstKTI GATC 5 cut(s) 256, 911, 1390, 1600, 1787
BstMAI GTCTC 3 cut(s) 196, 1720, 1730
BstMBI GATC 5 cut(s) 253, 908, 1387, 1597, 1784
BstMCI CGRYCG 1 cut(s) 1195
BstMWI GCNNNNNNNGC 2 cut(s) 1278, 1523
BstNI CCWGG 2 cut(s) 1418, 1668
BstNSI RCATGY 4 cut(s) 201, 1025, 1087, 1491
BstSCI CCNGG 2 cut(s) 1416, 1666
BstUI CGCG 1 cut(s) 880
BstV1I GCAGC 3 cut(s) 514, 518, 1459
BstX2I RGATCY 1 cut(s) 908
BstYI RGATCY 1 cut(s) 908
BstZ17I GTATAC 1 cut(s) 1027
BsuI GTATCC 1 cut(s) 1639
BsuRI GGCC 3 cut(s) 47, 110, 1314
BtgI CCRYGG 1 cut(s) 151
BtgZI GCGATG 1 cut(s) 70
BtsCI GGATG 2 cut(s) 927, 1729
BtsIMutI CAGTG 1 cut(s) 488
Cac8I GCNNGC 1 cut(s) 199
CaiI CAGNNNCTG 1 cut(s) 1460
CciI TCATGA 1 cut(s) 1128
CfoI GCGC 3 cut(s) 203, 630, 880
Cfr13I GGNCC 2 cut(s) 46, 1414
CseI GACGC 1 cut(s) 1407
Csp6I GTAC 5 cut(s) 172, 595, 1045, 1287, 1799
CviQI GTAC 5 cut(s) 172, 595, 1045, 1287, 1799
DpnI GATC 5 cut(s) 255, 910, 1389, 1599, 1786
DpnII GATC 5 cut(s) 253, 908, 1387, 1597, 1784
DraIII CACNNNGTG 1 cut(s) 235
DrdI GACNNNNNNGTC 2 cut(s) 973, 1404
DseDI GACNNNNNNGTC 2 cut(s) 973, 1404
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Ecl136II GAGCTC 1 cut(s) 998
Eco130I CCWWGG 1 cut(s) 1716
Eco24I GRGCYC 1 cut(s) 1000
Eco47I GGWCC 1 cut(s) 1414
Eco53kI GAGCTC 1 cut(s) 998
Eco57I CTGAAG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 998
EcoNI CCTNNNNNAGG 1 cut(s) 1682
EcoRII CCWGG 2 cut(s) 1416, 1666
EcoT14I CCWWGG 1 cut(s) 1716
EcoT38I GRGCYC 1 cut(s) 1000
ErhI CCWWGG 1 cut(s) 1716
FalI AAGNNNNNCTT 2 cut(s) 1299, 1331
FaqI GGGAC 2 cut(s) 528, 809
FauNDI CATATG 1 cut(s) 475
FblI GTMKAC 1 cut(s) 1026
Fnu4HI GCNGC 4 cut(s) 507, 528, 714, 1473
FokI GGATG 2 cut(s) 934, 1716
FriOI GRGCYC 1 cut(s) 1000
Fsp4HI GCNGC 4 cut(s) 507, 528, 714, 1473
FspBI CTAG 3 cut(s) 717, 992, 1118
GlaI GCGC 3 cut(s) 202, 629, 879
GluI GCNGC 4 cut(s) 507, 528, 714, 1473
GsaI CCCAGC 1 cut(s) 513
GsuI CTGGAG 1 cut(s) 1832
HaeII RGCGCY 1 cut(s) 631
HaeIII GGCC 3 cut(s) 47, 110, 1314
HapII CCGG 1 cut(s) 647
HgaI GACGC 1 cut(s) 1407
HhaI GCGC 3 cut(s) 203, 630, 880
Hin6I GCGC 3 cut(s) 201, 628, 878
HinP1I GCGC 3 cut(s) 201, 628, 878
HincII GTYRAC 1 cut(s) 1770
HindII GTYRAC 1 cut(s) 1770
HinfI GANTC 2 cut(s) 66, 307
HpaII CCGG 1 cut(s) 647
HphI GGTGA 7 cut(s) 116, 437, 557, 620, 1252, 1607, 1699
Hpy166II GTNNAC 3 cut(s) 576, 1027, 1770
Hpy188I TCNGA 4 cut(s) 135, 287, 1362, 1494
Hpy188III TCNNGA 8 cut(s) 385, 647, 1001, 1129, 1733, 1791, 1811, 1835
Hpy8I GTNNAC 3 cut(s) 576, 1027, 1770
HpyAV CCTTC 2 cut(s) 416, 1273
HpyCH4III ACNGT 6 cut(s) 16, 155, 769, 891, 970, 1193
HpyCH4IV ACGT 1 cut(s) 652
HpyF10VI GCNNNNNNNGC 2 cut(s) 1278, 1523
HpySE526I ACGT 1 cut(s) 652
HspAI GCGC 3 cut(s) 201, 628, 878
Kpn2I TCCGGA 1 cut(s) 646
Kzo9I GATC 5 cut(s) 253, 908, 1387, 1597, 1784
LguI GCTCTTC 1 cut(s) 338
LmnI GCTCC 7 cut(s) 464, 524, 649, 995, 1199, 1813, 1826
Lsp1109I GCAGC 3 cut(s) 514, 518, 1459
LweI GCATC 8 cut(s) 819, 912, 943, 1195, 1243, 1300, 1431, 1447
MaeI CTAG 3 cut(s) 717, 992, 1118
MaeII ACGT 1 cut(s) 652
MaeIII GTNAC 2 cut(s) 686, 964
MalI GATC 5 cut(s) 255, 910, 1389, 1599, 1786
MboI GATC 5 cut(s) 253, 908, 1387, 1597, 1784
MboII GAAGA 4 cut(s) 310, 355, 1319, 1450
MfeI CAATTG 1 cut(s) 364
MflI RGATCY 1 cut(s) 908
MhlI GDGCHC 2 cut(s) 1000, 1283
MluCI AATT 9 cut(s) 80, 129, 364, 1077, 1213, 1234, 1506, 1567, 1661
MlyI GAGTC 2 cut(s) 60, 316
MmeI TCCRAC 3 cut(s) 740, 1139, 1472
MroI TCCGGA 1 cut(s) 646
MroXI GAANNNNTTC 1 cut(s) 845
MseI TTAA 5 cut(s) 390, 588, 1076, 1151, 1211
MslI CAYNNNNRTG 3 cut(s) 962, 1133, 1194
MspA1I CMGCKG 1 cut(s) 509
MspI CCGG 1 cut(s) 647
MspR9I CCNGG 2 cut(s) 1418, 1668
MunI CAATTG 1 cut(s) 364
Mva1269I GAATGC 1 cut(s) 1075
MvaI CCWGG 2 cut(s) 1418, 1668
MvnI CGCG 1 cut(s) 880
MwoI GCNNNNNNNGC 2 cut(s) 1278, 1523
NdeI CATATG 1 cut(s) 475
NdeII GATC 5 cut(s) 253, 908, 1387, 1597, 1784
NlaIV GGNNCC 3 cut(s) 645, 1756, 1828
NmuCI GTSAC 1 cut(s) 964
NspI RCATGY 4 cut(s) 201, 1025, 1087, 1491
OliI CACNNNNGTG 2 cut(s) 962, 1194
PaeI GCATGC 1 cut(s) 201
PagI TCATGA 1 cut(s) 1128
PciI ACATGT 2 cut(s) 1021, 1083
PciSI GCTCTTC 1 cut(s) 338
PctI GAATGC 1 cut(s) 1075
PdmI GAANNNNTTC 1 cut(s) 845
PflMI CCANNNNNTGG 1 cut(s) 1333
PkrI GCNGC 4 cut(s) 508, 529, 715, 1474
PleI GAGTC 2 cut(s) 60, 315
PpsI GAGTC 2 cut(s) 60, 315
PscI ACATGT 2 cut(s) 1021, 1083
PshBI ATTAAT 1 cut(s) 588
Psp124BI GAGCTC 1 cut(s) 1000
Psp6I CCWGG 2 cut(s) 1416, 1666
PspFI CCCAGC 1 cut(s) 509
PspGI CCWGG 2 cut(s) 1416, 1666
PspN4I GGNNCC 3 cut(s) 645, 1756, 1828
PspPI GGNCC 2 cut(s) 46, 1414
PstNI CAGNNNCTG 1 cut(s) 1460
PsuI RGATCY 1 cut(s) 908
PvuII CAGCTG 1 cut(s) 509
RsaI GTAC 5 cut(s) 173, 596, 1046, 1288, 1800
RsaNI GTAC 5 cut(s) 172, 595, 1045, 1287, 1799
RseI CAYNNNNRTG 3 cut(s) 962, 1133, 1194
SacI GAGCTC 1 cut(s) 1000
SapI GCTCTTC 1 cut(s) 338
SaqAI TTAA 5 cut(s) 390, 588, 1076, 1151, 1211
SatI GCNGC 4 cut(s) 507, 528, 714, 1473
Sau3AI GATC 5 cut(s) 253, 908, 1387, 1597, 1784
Sau96I GGNCC 2 cut(s) 46, 1414
SchI GAGTC 2 cut(s) 60, 316
ScrFI CCNGG 2 cut(s) 1418, 1668
SduI GDGCHC 2 cut(s) 1000, 1283
SfaNI GCATC 8 cut(s) 819, 912, 943, 1195, 1243, 1300, 1431, 1447
SinI GGWCC 1 cut(s) 1414
SmiMI CAYNNNNRTG 3 cut(s) 962, 1133, 1194
SphI GCATGC 1 cut(s) 201
Sse9I AATT 9 cut(s) 80, 129, 364, 1077, 1213, 1234, 1506, 1567, 1661
SsiI CCGC 3 cut(s) 399, 713, 919
SspMI CTAG 3 cut(s) 717, 992, 1118
SstI GAGCTC 1 cut(s) 1000
StyD4I CCNGG 2 cut(s) 1416, 1666
StyI CCWWGG 1 cut(s) 1716
TaaI ACNGT 6 cut(s) 16, 155, 769, 891, 970, 1193
TaiI ACGT 1 cut(s) 655
TaqI TCGA 3 cut(s) 1305, 1542, 1548
TasI AATT 9 cut(s) 80, 129, 364, 1077, 1213, 1234, 1506, 1567, 1661
TatI WGTACW 1 cut(s) 171
TauI GCSGC 1 cut(s) 716
Tru1I TTAA 5 cut(s) 390, 588, 1076, 1151, 1211
Tru9I TTAA 5 cut(s) 390, 588, 1076, 1151, 1211
TscAI CASTG 1 cut(s) 495
TseFI GTSAC 1 cut(s) 964
TseI GCWGC 3 cut(s) 506, 527, 1472
Tsp45I GTSAC 1 cut(s) 964
TspRI CASTG 1 cut(s) 495
Van91I CCANNNNNTGG 1 cut(s) 1333
VpaK11BI GGWCC 1 cut(s) 1414
VspI ATTAAT 1 cut(s) 588
XagI CCTNNNNNAGG 1 cut(s) 1682
XapI RAATTY 4 cut(s) 80, 129, 1213, 1661
XceI RCATGY 4 cut(s) 201, 1025, 1087, 1491
XmiI GTMKAC 1 cut(s) 1026
XmnI GAANNNNTTC 1 cut(s) 845
XspI CTAG 3 cut(s) 717, 992, 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.