MD15G1443300.v1.1

UPF0420 protein C16orf58 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014565
Physical Location & Seq
Forward (+)
54338392 .. 54345335
6944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1443300.v1.1.491

Sequence Viewer

Length: 1572 bp
ATGCCTCCGCCGCCGCTGAAACTCAAGCAATCTTCTGCTTCTTCCACCAATCAAACCCTCACCACCACCGCCACTAGCGCCCAGGACGCGCGCCTTCTCGTCCGTGAAACGCTGCGTATCAGTGCAAACCTCGCTTCCTCCACTCCCTCCCAGGCTCTGCCTTTGGACACCCAAGCCCCCAAGCATCAGCTTCCCCAGCTGCTCCACCACGATCAAGATTTCGTGGGATCCAGCCTCAGATTGGTTTGCTGCGAGGAGATTGACGGCCGCCGATGGAACTACGTCGCTGAGCCCGATTCCTCCTCCTCCTCCTCCTCCTCTGCTGCAACCACCTTCAAGAAGGGCTCTTTTCGCGCCCTTAGCTTGCACAGCCCCCAACCCCCTCTCGACGGGTTGATGTCTTTCGTAAGGTCATATGTTGTCCCCGAAGGTTTCCCTGATAGTGTAATCCCTTCCTATGTCCCCTACATGACATGGAGAGCTCTCAAGCACTTCTTTGGTGGCGCAATGGGAGTTTTTACAACACAAACCCTTTTGAATTCAGTTGGAGTCTCTAGAAACACAGCTGCTCCTGGGGCTGTTGCTATCAACTGGATTCTCAAGGATGGTGCTGGGCGTGTTGGAAAAATGCTTTTTGCCCGACAAGGAAAGAAATTTGATTATGATCTAAAACAGTTGCGGTTTGCTGGTGATCTTCTCATGGAGTTGGGTGCAGGAGTAGAGTTGGCAACTGCAGCTGTCCCGCACATGTTTCTTCCTTTGGCTTGTGCAGCTAATGTGGCAAAGAATGTTGCTGCTGTAACATCAACATCAACTCGAACACCAATTTACAAAGCGTTTGCAAAAGGAGAAAACATTGGAGATGTGACAGCTAAAGGAGAATGTGTTGGCAATATTGCGGATCTGCTGGGAACTGGCCTGAGCATATTAATCTCGAAAAGAAATCCATCACTGATAACTACATTTGCGCTCCTTTCATGTGGATACGTTGTCAGCTCCTACCAAGAGGTTAAATCTGTAGTACTGCACACCTTGAACCGAGCGAGATTCAGTGTGGCAGTAGATGCCTTTCTTAAAACAGGGCGAGTGCCGTCGTTGCAGGAAGGGAACTTGAAGGAAAATGTTTTCAATTTTCCCTGGGTGAAAGATGGGCCTGTTGTTCTCGGACCACGATTTAAAGATGCATTCCAAGAGCCAAGTGTATATCTTGCGATAGAGCCCTTCTTTGAGAAAGAGAGATATGTTGTGACATATAATCCTTCAAAAGGTAAAGTGTACGCATTGTTCAAAGATCAAGCGAAGTCAGATGACATACTAAAGGCGGCATTTCATGCTCAAGTACTTCTATATTTCATGCAATCATCATACAACAGTCAGCATATGTATCAGAAGGAGAACAAAGATGGATCTTTAAACTTCGAGCCCACAGCTAAAGATCTGGAGGCGTGCATTGTTGAGTCATGTAAGATGGTCTCAACTTCGTATGGGATTTTCAAGAGTAAAGCTAAAGAGCAGGGATGGATGATGTCAGAAGCACATCTCAATCCTGGCCGAGCTCGTCTGATTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF647

Protein Analysis

524

Amino Acids

57.14

Weight (kDa)

9.24

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RUS6_N PF24162 25 - 126 9.6e-33 Protein root UVB sensitive 6, N-terminal
UVB_sens_prot PF04884 132 - 360 8.2e-76 Vitamin B6 photo-protection and homoeostasis domain
UVB_sens_C PF24160 362 - 516 3.5e-14 Root UVB sensitive protein, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49820
fragaria_vesca FvH4_5g27690
malus_domestica MD08G1248500.v1.1 MD15G1443300.v1.1
prunus_persica Prupe.1G585900_v2.0.a1
pyrus_communis pycom08g21530 pycom15g39100
rosa_chinensis RchiOBHm_Chr7g0219391
rosa_laevigata RLG00000002291
rosa_multiflora Rmu_sc0002460.1_g000012
rosa_roxburghii Rroxscaffold_3G00240060
rosa_rugosa Rorug07G0184800
rosa_samantha Rh7AG327700 Rh7CG345000 Rh7DG323100
rosa_wichuraiana Rw7G027590 Rw7G027680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 89, 91, 354
AciI CCGC 9 cut(s) 8, 11, 14, 69, 268, 679, 743, 899, 1322
AclWI GGATC 4 cut(s) 222, 235, 909, 1414
AcoI YGGCCR 2 cut(s) 265, 1549
AcsI RAATTY 2 cut(s) 538, 653
AfaI GTAC 3 cut(s) 1023, 1277, 1341
AfiI CCNNNNNNNGG 3 cut(s) 241, 389, 1265
AflIII ACRYGT 1 cut(s) 747
AgsI TTSAA 8 cut(s) 337, 538, 1036, 1114, 1129, 1263, 1288, 1495
AjnI CCWGG 5 cut(s) 81, 150, 571, 1136, 1546
Alw21I GWGCWC 2 cut(s) 484, 1558
Alw26I GTCTC 2 cut(s) 556, 1477
AlwI GGATC 4 cut(s) 222, 235, 909, 1414
AlwNI CAGNNNCTG 1 cut(s) 157
AoxI GGCC 4 cut(s) 265, 916, 1151, 1549
ApeKI GCWGC 8 cut(s) 112, 199, 249, 323, 566, 734, 770, 794
ApoI RAATTY 2 cut(s) 538, 653
AseI ATTAAT 1 cut(s) 929
AspLEI GCGC 6 cut(s) 80, 91, 93, 356, 506, 970
AspS9I GGNCC 2 cut(s) 1151, 1166
AsuHPI GGTGA 3 cut(s) 52, 701, 1153
AvaII GGWCC 1 cut(s) 1166
BamHI GGATCC 1 cut(s) 227
BanII GRGCYC 6 cut(s) 294, 347, 484, 1221, 1425, 1558
Bbv12I GWGCWC 2 cut(s) 484, 1558
BbvI GCAGC 8 cut(s) 99, 186, 236, 310, 553, 746, 781, 782
BccI CCATC 7 cut(s) 267, 599, 955, 1142, 1397, 1462, 1512
BceAI ACGGC 2 cut(s) 280, 1075
BciT130I CCWGG 5 cut(s) 83, 152, 573, 1138, 1548
BciVI GTATCC 1 cut(s) 977
BcoDI GTCTC 2 cut(s) 556, 1477
BfaI CTAG 2 cut(s) 75, 555
BfmI CTRYAG 2 cut(s) 732, 1017
BfoI RGCGCY 1 cut(s) 81
BfuI GTATCC 1 cut(s) 977
BglII AGATCT 1 cut(s) 1435
BlpI GCTNAGC 1 cut(s) 288
BmcAI AGTACT 2 cut(s) 1023, 1341
Bme1390I CCNGG 5 cut(s) 83, 152, 573, 1138, 1548
Bme18I GGWCC 1 cut(s) 1166
BmgT120I GGNCC 2 cut(s) 1151, 1166
BmiI GGNNCC 1 cut(s) 229
BmrFI CCNGG 5 cut(s) 83, 152, 573, 1138, 1548
BmsI GCATC 3 cut(s) 193, 1054, 1171
BpmI CTGGAG 1 cut(s) 1460
Bpu10I CCTNAGC 2 cut(s) 359, 920
Bpu1102I GCTNAGC 1 cut(s) 288
BpuEI CTTGAG 4 cut(s) 8, 470, 584, 1320
BsaBI GATNNNNATC 1 cut(s) 663
BsaI GGTCTC 1 cut(s) 1477
BsaJI CCNNGG 5 cut(s) 81, 150, 572, 1136, 1137
BsaXI ACNNNNNCTCC 4 cut(s) 553, 583, 870, 900
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 389, 1265
Bse1I ACTGG 2 cut(s) 596, 919
Bse3DI GCAATG 1 cut(s) 513
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 5 cut(s) 83, 152, 573, 1138, 1548
BseDI CCNNGG 5 cut(s) 81, 150, 572, 1136, 1137
BseGI GGATG 3 cut(s) 610, 1523, 1527
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 3 cut(s) 241, 389, 1265
BseMI GCAATG 1 cut(s) 513
BseMII CTCAG 3 cut(s) 250, 279, 911
BseNI ACTGG 2 cut(s) 596, 919
BsePI GCGCGC 1 cut(s) 89
BseRI GAGGAG 7 cut(s) 269, 292, 295, 298, 301, 304, 307
BseX3I CGGCCG 1 cut(s) 265
BseXI GCAGC 8 cut(s) 99, 186, 236, 310, 553, 746, 781, 782
BseYI CCCAGC 3 cut(s) 195, 611, 907
BsgI GTGCAG 3 cut(s) 732, 789, 1010
Bsh1236I CGCG 3 cut(s) 89, 91, 354
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 4 cut(s) 267, 918, 1153, 1551
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 2 cut(s) 484, 1558
BslFI GGGAC 3 cut(s) 407, 446, 725
BslI CCNNNNNNNGG 3 cut(s) 241, 389, 1265
BsmAI GTCTC 2 cut(s) 556, 1477
BsmFI GGGAC 3 cut(s) 407, 446, 725
BsmI GAATGC 1 cut(s) 1184
BsnI GGCC 4 cut(s) 267, 918, 1153, 1551
Bso31I GGTCTC 1 cut(s) 1477
Bsp1286I GDGCHC 6 cut(s) 294, 347, 484, 1221, 1425, 1558
Bsp143I GATC 8 cut(s) 211, 227, 664, 691, 901, 1291, 1406, 1435
Bsp1720I GCTNAGC 1 cut(s) 288
BspACI CCGC 9 cut(s) 8, 11, 14, 69, 268, 679, 743, 899, 1322
BspANI GGCC 4 cut(s) 267, 918, 1153, 1551
BspCNI CTCAG 3 cut(s) 249, 280, 912
BspFNI CGCG 3 cut(s) 89, 91, 354
BspLI GGNNCC 1 cut(s) 229
BspMAI CTGCAG 1 cut(s) 736
BspPI GGATC 4 cut(s) 222, 235, 909, 1414
BspTNI GGTCTC 1 cut(s) 1477
BsrDI GCAATG 1 cut(s) 513
BsrI ACTGG 2 cut(s) 596, 919
BssECI CCNNGG 5 cut(s) 81, 150, 572, 1136, 1137
BssHII GCGCGC 1 cut(s) 89
BssMI GATC 8 cut(s) 211, 227, 664, 691, 901, 1291, 1406, 1435
Bst2UI CCWGG 5 cut(s) 83, 152, 573, 1138, 1548
Bst4CI ACNGT 2 cut(s) 675, 1373
BstAPI GCANNNNNTGC 2 cut(s) 1064, 1331
BstC8I GCNNGC 3 cut(s) 91, 365, 1447
BstDEI CTNAG 4 cut(s) 236, 288, 359, 920
BstENI CCTNNNNNAGG 1 cut(s) 1263
BstF5I GGATG 3 cut(s) 610, 1523, 1527
BstFNI CGCG 3 cut(s) 89, 91, 354
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 6 cut(s) 80, 91, 93, 356, 506, 970
BstKTI GATC 8 cut(s) 214, 230, 667, 694, 904, 1294, 1409, 1438
BstMAI GTCTC 2 cut(s) 556, 1477
BstMBI GATC 8 cut(s) 211, 227, 664, 691, 901, 1291, 1406, 1435
BstMCI CGRYCG 1 cut(s) 268
BstNI CCWGG 5 cut(s) 83, 152, 573, 1138, 1548
BstNSI RCATGY 1 cut(s) 751
BstSCI CCNGG 5 cut(s) 81, 150, 571, 1136, 1546
BstSFI CTRYAG 2 cut(s) 732, 1017
BstUI CGCG 3 cut(s) 89, 91, 354
BstV1I GCAGC 8 cut(s) 99, 186, 236, 310, 553, 746, 781, 782
BstX2I RGATCY 4 cut(s) 227, 901, 1406, 1435
BstYI RGATCY 4 cut(s) 227, 901, 1406, 1435
BstZI CGGCCG 1 cut(s) 265
BsuI GTATCC 1 cut(s) 977
BsuRI GGCC 4 cut(s) 267, 918, 1153, 1551
BtsCI GGATG 3 cut(s) 610, 1523, 1527
BtsIMutI CAGTG 3 cut(s) 127, 950, 1057
Cac8I GCNNGC 3 cut(s) 91, 365, 1447
CaiI CAGNNNCTG 1 cut(s) 157
CfoI GCGC 6 cut(s) 80, 91, 93, 356, 506, 970
Cfr13I GGNCC 2 cut(s) 1151, 1166
CseI GACGC 1 cut(s) 95
Csp6I GTAC 3 cut(s) 1022, 1276, 1340
CviAII CATG 8 cut(s) 469, 474, 700, 748, 978, 1331, 1354, 1461
CviQI GTAC 3 cut(s) 1022, 1276, 1340
DdeI CTNAG 4 cut(s) 236, 288, 359, 920
DpnI GATC 8 cut(s) 213, 229, 666, 693, 903, 1293, 1408, 1437
DpnII GATC 8 cut(s) 211, 227, 664, 691, 901, 1291, 1406, 1435
DraI TTTAAA 2 cut(s) 1177, 1413
EaeI YGGCCR 2 cut(s) 265, 1549
EagI CGGCCG 1 cut(s) 265
Ecl136II GAGCTC 2 cut(s) 482, 1556
EclXI CGGCCG 1 cut(s) 265
Eco24I GRGCYC 6 cut(s) 294, 347, 484, 1221, 1425, 1558
Eco31I GGTCTC 1 cut(s) 1477
Eco47I GGWCC 1 cut(s) 1166
Eco52I CGGCCG 1 cut(s) 265
Eco53kI GAGCTC 2 cut(s) 482, 1556
EcoICRI GAGCTC 2 cut(s) 482, 1556
EcoNI CCTNNNNNAGG 1 cut(s) 1263
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 5 cut(s) 81, 150, 571, 1136, 1546
EcoT22I ATGCAT 1 cut(s) 1186
EcoT38I GRGCYC 6 cut(s) 294, 347, 484, 1221, 1425, 1558
FaeI CATG 8 cut(s) 472, 477, 703, 751, 981, 1334, 1357, 1464
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 3 cut(s) 407, 446, 725
FatI CATG 8 cut(s) 468, 473, 699, 747, 977, 1330, 1353, 1460
FauI CCCGC 1 cut(s) 750
FauNDI CATATG 2 cut(s) 415, 1380
FokI GGATG 3 cut(s) 617, 1530, 1534
FriOI GRGCYC 6 cut(s) 294, 347, 484, 1221, 1425, 1558
FspBI CTAG 2 cut(s) 75, 555
GlaI GCGC 6 cut(s) 79, 90, 92, 355, 505, 969
GsaI CCCAGC 3 cut(s) 199, 615, 911
GsuI CTGGAG 1 cut(s) 1460
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 4 cut(s) 267, 918, 1153, 1551
HgaI GACGC 1 cut(s) 95
HhaI GCGC 6 cut(s) 80, 91, 93, 356, 506, 970
Hin1II CATG 8 cut(s) 472, 477, 703, 751, 981, 1334, 1357, 1464
Hin6I GCGC 6 cut(s) 78, 89, 91, 354, 504, 968
HinP1I GCGC 6 cut(s) 78, 89, 91, 354, 504, 968
HinfI GANTC 5 cut(s) 296, 549, 595, 1047, 1457
HphI GGTGA 3 cut(s) 52, 701, 1153
Hpy166II GTNNAC 1 cut(s) 1276
Hpy188I TCNGA 6 cut(s) 239, 1166, 1306, 1389, 1531, 1563
Hpy188III TCNNGA 7 cut(s) 215, 337, 386, 555, 934, 1439, 1495
Hpy8I GTNNAC 1 cut(s) 1276
Hpy99I CGWCG 3 cut(s) 287, 392, 1096
HpyCH4III ACNGT 2 cut(s) 675, 1373
HpyCH4IV ACGT 2 cut(s) 282, 987
HpyF3I CTNAG 4 cut(s) 236, 288, 359, 920
HpySE526I ACGT 2 cut(s) 282, 987
Hsp92II CATG 8 cut(s) 472, 477, 703, 751, 981, 1334, 1357, 1464
HspAI GCGC 6 cut(s) 78, 89, 91, 354, 504, 968
Kzo9I GATC 8 cut(s) 211, 227, 664, 691, 901, 1291, 1406, 1435
LmnI GCTCC 4 cut(s) 207, 574, 975, 1001
Lsp1109I GCAGC 8 cut(s) 99, 186, 236, 310, 553, 746, 781, 782
LweI GCATC 3 cut(s) 193, 1054, 1171
MaeI CTAG 2 cut(s) 75, 555
MaeII ACGT 2 cut(s) 282, 987
MaeIII GTNAC 3 cut(s) 799, 865, 1246
MalI GATC 8 cut(s) 213, 229, 666, 693, 903, 1293, 1408, 1437
MboI GATC 8 cut(s) 211, 227, 664, 691, 901, 1291, 1406, 1435
MboII GAAGA 4 cut(s) 24, 33, 686, 746
MflI RGATCY 4 cut(s) 227, 901, 1406, 1435
MhlI GDGCHC 6 cut(s) 294, 347, 484, 1221, 1425, 1558
MluCI AATT 4 cut(s) 538, 653, 825, 1129
MlyI GAGTC 2 cut(s) 558, 1466
MmeI TCCRAC 2 cut(s) 526, 601
Mph1103I ATGCAT 1 cut(s) 1186
MseI TTAA 6 cut(s) 929, 1011, 1074, 1176, 1412, 1566
MspA1I CMGCKG 4 cut(s) 16, 199, 566, 737
MspR9I CCNGG 5 cut(s) 83, 152, 573, 1138, 1548
Mva1269I GAATGC 1 cut(s) 1184
MvaI CCWGG 5 cut(s) 83, 152, 573, 1138, 1548
MvnI CGCG 3 cut(s) 89, 91, 354
NdeI CATATG 2 cut(s) 415, 1380
NdeII GATC 8 cut(s) 211, 227, 664, 691, 901, 1291, 1406, 1435
NlaIII CATG 8 cut(s) 472, 477, 703, 751, 981, 1334, 1357, 1464
NlaIV GGNNCC 1 cut(s) 229
NmuCI GTSAC 2 cut(s) 865, 1246
NsiI ATGCAT 1 cut(s) 1186
NspI RCATGY 1 cut(s) 751
PasI CCCWGGG 1 cut(s) 1137
PauI GCGCGC 1 cut(s) 89
PciI ACATGT 1 cut(s) 747
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 1 cut(s) 1184
PfeI GAWTC 3 cut(s) 296, 595, 1047
PleI GAGTC 2 cut(s) 557, 1465
PpsI GAGTC 2 cut(s) 557, 1465
PscI ACATGT 1 cut(s) 747
PshBI ATTAAT 1 cut(s) 929
Psp124BI GAGCTC 2 cut(s) 484, 1558
Psp6I CCWGG 5 cut(s) 81, 150, 571, 1136, 1546
PspFI CCCAGC 3 cut(s) 195, 611, 907
PspGI CCWGG 5 cut(s) 81, 150, 571, 1136, 1546
PspN4I GGNNCC 1 cut(s) 229
PspPI GGNCC 2 cut(s) 1151, 1166
PsrI GAACNNNNNNTAC 2 cut(s) 1268, 1300
PstI CTGCAG 1 cut(s) 736
PstNI CAGNNNCTG 1 cut(s) 157
PsuI RGATCY 4 cut(s) 227, 901, 1406, 1435
PteI GCGCGC 1 cut(s) 89
PvuII CAGCTG 3 cut(s) 199, 566, 737
RsaI GTAC 3 cut(s) 1023, 1277, 1341
RsaNI GTAC 3 cut(s) 1022, 1276, 1340
SacI GAGCTC 2 cut(s) 484, 1558
SaqAI TTAA 6 cut(s) 929, 1011, 1074, 1176, 1412, 1566
Sau3AI GATC 8 cut(s) 211, 227, 664, 691, 901, 1291, 1406, 1435
Sau96I GGNCC 2 cut(s) 1151, 1166
ScaI AGTACT 2 cut(s) 1023, 1341
SchI GAGTC 2 cut(s) 558, 1466
ScrFI CCNGG 5 cut(s) 83, 152, 573, 1138, 1548
SduI GDGCHC 6 cut(s) 294, 347, 484, 1221, 1425, 1558
SfaNI GCATC 3 cut(s) 193, 1054, 1171
SfcI CTRYAG 2 cut(s) 732, 1017
SinI GGWCC 1 cut(s) 1166
SmlI CTYRAG 4 cut(s) 23, 485, 599, 1335
SmoI CTYRAG 4 cut(s) 23, 485, 599, 1335
Sse9I AATT 4 cut(s) 538, 653, 825, 1129
SsiI CCGC 9 cut(s) 8, 11, 14, 69, 268, 679, 743, 899, 1322
SspI AATATT 1 cut(s) 895
SspMI CTAG 2 cut(s) 75, 555
SstI GAGCTC 2 cut(s) 484, 1558
StyD4I CCNGG 5 cut(s) 81, 150, 571, 1136, 1546
TaaI ACNGT 2 cut(s) 675, 1373
TaiI ACGT 2 cut(s) 285, 990
TaqI TCGA 4 cut(s) 387, 817, 935, 1419
TasI AATT 4 cut(s) 538, 653, 825, 1129
TatI WGTACW 2 cut(s) 1021, 1339
TauI GCSGC 4 cut(s) 13, 16, 270, 1325
TfiI GAWTC 3 cut(s) 296, 595, 1047
Tru1I TTAA 6 cut(s) 929, 1011, 1074, 1176, 1412, 1566
Tru9I TTAA 6 cut(s) 929, 1011, 1074, 1176, 1412, 1566
TscAI CASTG 3 cut(s) 127, 957, 1057
TseFI GTSAC 2 cut(s) 865, 1246
TseI GCWGC 8 cut(s) 112, 199, 249, 323, 566, 734, 770, 794
Tsp45I GTSAC 2 cut(s) 865, 1246
TspDTI ATGAA 3 cut(s) 966, 1319, 1342
TspGWI ACGGA 1 cut(s) 92
TspRI CASTG 3 cut(s) 127, 957, 1057
VpaK11BI GGWCC 1 cut(s) 1166
VspI ATTAAT 1 cut(s) 929
XagI CCTNNNNNAGG 1 cut(s) 1263
XapI RAATTY 2 cut(s) 538, 653
XbaI TCTAGA 1 cut(s) 554
XceI RCATGY 1 cut(s) 751
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XspI CTAG 2 cut(s) 75, 555
ZrmI AGTACT 2 cut(s) 1023, 1341
Zsp2I ATGCAT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.