pycom08g21530

UPF0420 protein C16orf58 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014565
Physical Location & Seq
Forward (+)
20982919 .. 20987395
4477 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g21530.5

Sequence Viewer

Length: 1557 bp
ATGCCTCCGCCCCCGATGAAACTCAAGCAATCTTCTGCTTCCGCTTCTTCTACCAATCAATCCCTCACCACCACCGCCACCAGCGCGCAGGAAGCGCGCCTTCTCGTCCATGAGACGCTGCGTATCAGTGCCACCCTCGCTTCCTCCGCTCCCTCCCAGTCCCACGCCCAACCCGCCAAGCAGCATCTTCCCCAGCTGCTCCACCACGATCAGGATTTTGTGGGCTCCAGCTTCCGTTTGATTTGCTGCGAGGAGATTGACGGCCGCCGATGGAACTACGTCGCCGAGCCCGATTCCTCCTCCTCCTCTGCAACCACCTTCAAGAAGGGCTCTTTTCGCGCCCTTAGCTTGCACAGCCCCCAGCCCCCTCTCGACGGGTTGATGTCTTTTGTAAGGTCATATGTTGTCCCCGAAGGTTTCCCTGATAGTGTAATCCCTTCCTATGTCCCCTACATGACATGGAGAGCTCTCAAGCACTTCTTTGGTGGCGCAATGGGTGTTTTTACAACACAAACCCTTTTGAATTCAGTTGGGGTCTCTAGAAATACATCTGCTTCTGGGGCTGTTGCTATCAACTGGATTCTCAAGGATGGTGCTGGGCGTGTTGGAAAAATGCTTTTTGCCCGACAGGGAAAGAAATTTGATTATGATCTAAAACAGTTGCGGTTTGCTGGTGATCTTCTCATGGAATTGGGTGCTGGAATAGAGTTGGCAACTGCAGCTGTCCCTCACCTGTTTCTTCCTTTGGCTTGTGCAGCTAATGTGGCAAAGAATGTTGGTGCTGTTACATCAACATCAACTCGAACACCAATTTACAAAGCGTTTGCAAAAGGAGAAAACATTGGAGATGTGACAGCTAAGGGAGAATGTGTTGGCAATATTGCAGATCTGCTGGGAACTGGCCTGAGTATATTGATCTCAAAAAGAAATCCATCGCTGATAACTACATTTGCGCTCCTTTCATGTGGATATGTTCTCAGCTCCTACCAAGAGGTTAAATCTGTAGTATTACACACCTTGAACCGAGCAAGATTCGGTGTGGCAGTAGATGCTTTTCTTAAAACAGGGCGAGTGCCGTCATTGCTGGAAGGGAACTTGAAGGAAAACGTTTTCAATTTTCCCTGGGTGAAAGACGGGCCTGTTGTTCTTGGACCACGATTTAAAGATGCATTCCAAGAGCCAAGTGTATATATTGCGATAGAGCCCTTCTTTGAGAAAGAGAAATATGTTGTGACATATAATCCTTCAAAAGGTAAAGTGTACGCATTGTTCAAAGATCAAGCGAAGTCAGATGACATTCTAAAAGCGGCATTTCATGCTCAGGTACTTCTGCATTTCATGCAATTATCATATAACAGTCAGCATCTGTATCAGAAGGACAAGAAAGATGAATTTTTAAACTTCGAGCCCACGGCTAAAGATCTGGAGGCATGCATTGTTGAGTCATGTAACATGGTCTCAACTTCGTATGGGATTTTCAAGAGTAAAGCTAAAGAGCAGGGATGGATGATGTCAGAAGCACATCTCAATCCTGGCCGAGCTCGTCTGATTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF647

Protein Analysis

519

Amino Acids

56.68

Weight (kDa)

9.13

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49820
fragaria_vesca FvH4_5g27690
malus_domestica MD08G1248500.v1.1 MD15G1443300.v1.1
prunus_persica Prupe.1G585900_v2.0.a1
pyrus_communis pycom08g21530 pycom15g39100
rosa_chinensis RchiOBHm_Chr7g0219391
rosa_laevigata RLG00000002291
rosa_multiflora Rmu_sc0002460.1_g000012
rosa_roxburghii Rroxscaffold_3G00240060
rosa_rugosa Rorug07G0184800
rosa_samantha Rh7AG327700 Rh7CG345000 Rh7DG323100
rosa_wichuraiana Rw7G027590 Rw7G027680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 149
AccII CGCG 3 cut(s) 86, 97, 339
AciI CCGC 8 cut(s) 8, 42, 75, 147, 174, 265, 664, 1307
AclI AACGTT 1 cut(s) 1107
AcoI YGGCCR 2 cut(s) 262, 1534
AcsI RAATTY 3 cut(s) 523, 638, 1391
AfaI GTAC 2 cut(s) 1262, 1326
AfiI CCNNNNNNNGG 3 cut(s) 211, 374, 1250
AgsI TTSAA 8 cut(s) 322, 523, 1021, 1099, 1114, 1248, 1273, 1480
AjnI CCWGG 2 cut(s) 1121, 1531
Alw21I GWGCWC 2 cut(s) 469, 1543
Alw26I GTCTC 3 cut(s) 107, 541, 1462
AlwNI CAGNNNCTG 1 cut(s) 1366
AoxI GGCC 4 cut(s) 262, 901, 1136, 1534
ApeKI GCWGC 6 cut(s) 118, 181, 196, 246, 719, 755
ApoI RAATTY 3 cut(s) 523, 638, 1391
AspLEI GCGC 7 cut(s) 86, 88, 97, 99, 341, 491, 955
AspS9I GGNCC 2 cut(s) 1136, 1151
AsuHPI GGTGA 4 cut(s) 58, 686, 722, 1138
AvaII GGWCC 1 cut(s) 1151
BanII GRGCYC 7 cut(s) 227, 291, 332, 469, 1206, 1410, 1543
Bbv12I GWGCWC 2 cut(s) 469, 1543
BbvI GCAGC 6 cut(s) 105, 183, 193, 233, 731, 767
BccI CCATC 4 cut(s) 264, 584, 940, 1497
BceAI ACGGC 3 cut(s) 277, 1060, 1428
BciT130I CCWGG 2 cut(s) 1123, 1533
BcoDI GTCTC 3 cut(s) 107, 541, 1462
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 2 cut(s) 717, 1002
BglII AGATCT 2 cut(s) 886, 1420
BisI GCNGC 8 cut(s) 119, 182, 197, 247, 265, 720, 756, 1308
BlsI GCNGC 8 cut(s) 120, 183, 198, 248, 266, 721, 757, 1309
Bme1390I CCNGG 2 cut(s) 1123, 1533
Bme18I GGWCC 1 cut(s) 1151
BmgT120I GGNCC 2 cut(s) 1136, 1151
BmiI GGNNCC 1 cut(s) 226
BmrFI CCNGG 2 cut(s) 1123, 1533
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 4 cut(s) 193, 1039, 1156, 1372
BmuI ACTGGG 1 cut(s) 151
BpmI CTGGAG 2 cut(s) 211, 1445
Bpu10I CCTNAGC 3 cut(s) 344, 858, 1320
BpuEI CTTGAG 3 cut(s) 8, 455, 569
BsaBI GATNNNNATC 1 cut(s) 648
BsaI GGTCTC 2 cut(s) 541, 1462
BsaJI CCNNGG 3 cut(s) 1121, 1122, 1410
BsaXI ACNNNNNCTCC 2 cut(s) 855, 885
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 374, 1250
Bse1I ACTGG 3 cut(s) 157, 581, 904
Bse3DI GCAATG 2 cut(s) 498, 1079
Bse8I GATNNNNATC 1 cut(s) 648
BseBI CCWGG 2 cut(s) 1123, 1533
BseDI CCNNGG 3 cut(s) 1121, 1122, 1410
BseGI GGATG 3 cut(s) 595, 1508, 1512
BseJI GATNNNNATC 1 cut(s) 648
BseLI CCNNNNNNNGG 3 cut(s) 211, 374, 1250
BseMI GCAATG 2 cut(s) 498, 1079
BseMII CTCAG 3 cut(s) 896, 991, 1334
BseNI ACTGG 3 cut(s) 157, 581, 904
BsePI GCGCGC 2 cut(s) 84, 95
BseRI GAGGAG 4 cut(s) 266, 289, 292, 295
BseX3I CGGCCG 1 cut(s) 262
BseXI GCAGC 6 cut(s) 105, 183, 193, 233, 731, 767
BseYI CCCAGC 4 cut(s) 192, 360, 596, 892
BsgI GTGCAG 1 cut(s) 774
Bsh1236I CGCG 3 cut(s) 86, 97, 339
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 4 cut(s) 264, 903, 1138, 1536
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 2 cut(s) 469, 1543
BslFI GGGAC 4 cut(s) 145, 392, 431, 710
BslI CCNNNNNNNGG 3 cut(s) 211, 374, 1250
BsmAI GTCTC 3 cut(s) 107, 541, 1462
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 4 cut(s) 145, 392, 431, 710
BsmI GAATGC 1 cut(s) 1169
BsnI GGCC 4 cut(s) 264, 903, 1138, 1536
Bso31I GGTCTC 2 cut(s) 541, 1462
Bsp1286I GDGCHC 7 cut(s) 227, 291, 332, 469, 1206, 1410, 1543
Bsp143I GATC 7 cut(s) 208, 649, 676, 886, 915, 1276, 1420
BspACI CCGC 8 cut(s) 8, 42, 75, 147, 174, 265, 664, 1307
BspANI GGCC 4 cut(s) 264, 903, 1138, 1536
BspCNI CTCAG 3 cut(s) 897, 990, 1333
BspFNI CGCG 3 cut(s) 86, 97, 339
BspLI GGNNCC 1 cut(s) 226
BspMAI CTGCAG 1 cut(s) 721
BspTNI GGTCTC 2 cut(s) 541, 1462
BsrBI CCGCTC 1 cut(s) 149
BsrDI GCAATG 2 cut(s) 498, 1079
BsrI ACTGG 3 cut(s) 157, 581, 904
BssECI CCNNGG 3 cut(s) 1121, 1122, 1410
BssHII GCGCGC 2 cut(s) 84, 95
BssMI GATC 7 cut(s) 208, 649, 676, 886, 915, 1276, 1420
Bst2UI CCWGG 2 cut(s) 1123, 1533
Bst4CI ACNGT 2 cut(s) 660, 1358
BstAPI GCANNNNNTGC 3 cut(s) 1049, 1316, 1339
BstC8I GCNNGC 4 cut(s) 86, 97, 350, 1432
BstDEI CTNAG 5 cut(s) 344, 858, 905, 977, 1320
BstDSI CCRYGG 1 cut(s) 1410
BstENI CCTNNNNNAGG 1 cut(s) 1248
BstF5I GGATG 3 cut(s) 595, 1508, 1512
BstFNI CGCG 3 cut(s) 86, 97, 339
BstHHI GCGC 7 cut(s) 86, 88, 97, 99, 341, 491, 955
BstKTI GATC 7 cut(s) 211, 652, 679, 889, 918, 1279, 1423
BstMAI GTCTC 3 cut(s) 107, 541, 1462
BstMBI GATC 7 cut(s) 208, 649, 676, 886, 915, 1276, 1420
BstMCI CGRYCG 1 cut(s) 265
BstNI CCWGG 2 cut(s) 1123, 1533
BstNSI RCATGY 1 cut(s) 1434
BstSCI CCNGG 2 cut(s) 1121, 1531
BstSFI CTRYAG 2 cut(s) 717, 1002
BstUI CGCG 3 cut(s) 86, 97, 339
BstV1I GCAGC 6 cut(s) 105, 183, 193, 233, 731, 767
BstX2I RGATCY 2 cut(s) 886, 1420
BstYI RGATCY 2 cut(s) 886, 1420
BstZI CGGCCG 1 cut(s) 262
BsuRI GGCC 4 cut(s) 264, 903, 1138, 1536
BtgI CCRYGG 1 cut(s) 1410
BtgZI GCGATG 1 cut(s) 918
BtsCI GGATG 3 cut(s) 595, 1508, 1512
BtsIMutI CAGTG 1 cut(s) 133
Cac8I GCNNGC 4 cut(s) 86, 97, 350, 1432
CaiI CAGNNNCTG 1 cut(s) 1366
CfoI GCGC 7 cut(s) 86, 88, 97, 99, 341, 491, 955
Cfr13I GGNCC 2 cut(s) 1136, 1151
CseI GACGC 1 cut(s) 124
Csp6I GTAC 2 cut(s) 1261, 1325
CviQI GTAC 2 cut(s) 1261, 1325
DdeI CTNAG 5 cut(s) 344, 858, 905, 977, 1320
DpnI GATC 7 cut(s) 210, 651, 678, 888, 917, 1278, 1422
DpnII GATC 7 cut(s) 208, 649, 676, 886, 915, 1276, 1420
DraI TTTAAA 2 cut(s) 1162, 1398
EaeI YGGCCR 2 cut(s) 262, 1534
EagI CGGCCG 1 cut(s) 262
Ecl136II GAGCTC 2 cut(s) 467, 1541
EclXI CGGCCG 1 cut(s) 262
Eco24I GRGCYC 7 cut(s) 227, 291, 332, 469, 1206, 1410, 1543
Eco31I GGTCTC 2 cut(s) 541, 1462
Eco47I GGWCC 1 cut(s) 1151
Eco52I CGGCCG 1 cut(s) 262
Eco53kI GAGCTC 2 cut(s) 467, 1541
EcoICRI GAGCTC 2 cut(s) 467, 1541
EcoNI CCTNNNNNAGG 1 cut(s) 1248
EcoRI GAATTC 1 cut(s) 523
EcoRII CCWGG 2 cut(s) 1121, 1531
EcoT22I ATGCAT 2 cut(s) 1171, 1436
EcoT38I GRGCYC 7 cut(s) 227, 291, 332, 469, 1206, 1410, 1543
Esp3I CGTCTC 1 cut(s) 107
FalI AAGNNNNNCTT 4 cut(s) 84, 116, 464, 496
FaqI GGGAC 4 cut(s) 145, 392, 431, 710
FauI CCCGC 1 cut(s) 181
FauNDI CATATG 1 cut(s) 400
Fnu4HI GCNGC 8 cut(s) 119, 182, 197, 247, 265, 720, 756, 1308
FokI GGATG 3 cut(s) 602, 1515, 1519
FriOI GRGCYC 7 cut(s) 227, 291, 332, 469, 1206, 1410, 1543
Fsp4HI GCNGC 8 cut(s) 119, 182, 197, 247, 265, 720, 756, 1308
FspBI CTAG 1 cut(s) 540
GlaI GCGC 7 cut(s) 85, 87, 96, 98, 340, 490, 954
GluI GCNGC 8 cut(s) 119, 182, 197, 247, 265, 720, 756, 1308
GsaI CCCAGC 4 cut(s) 196, 364, 600, 896
GsuI CTGGAG 2 cut(s) 211, 1445
HaeIII GGCC 4 cut(s) 264, 903, 1138, 1536
HgaI GACGC 1 cut(s) 124
HhaI GCGC 7 cut(s) 86, 88, 97, 99, 341, 491, 955
Hin6I GCGC 7 cut(s) 84, 86, 95, 97, 339, 489, 953
HinP1I GCGC 7 cut(s) 84, 86, 95, 97, 339, 489, 953
HinfI GANTC 5 cut(s) 293, 580, 1032, 1442, 1549
HphI GGTGA 4 cut(s) 58, 686, 722, 1138
Hpy166II GTNNAC 1 cut(s) 1261
Hpy188I TCNGA 4 cut(s) 1291, 1374, 1516, 1548
Hpy188III TCNNGA 6 cut(s) 212, 322, 371, 540, 1424, 1480
Hpy8I GTNNAC 1 cut(s) 1261
Hpy99I CGWCG 2 cut(s) 284, 377
HpyCH4III ACNGT 2 cut(s) 660, 1358
HpyCH4IV ACGT 2 cut(s) 279, 1107
HpyF3I CTNAG 5 cut(s) 344, 858, 905, 977, 1320
HpySE526I ACGT 2 cut(s) 279, 1107
HspAI GCGC 7 cut(s) 84, 86, 95, 97, 339, 489, 953
Kzo9I GATC 7 cut(s) 208, 649, 676, 886, 915, 1276, 1420
LmnI GCTCC 5 cut(s) 154, 204, 230, 960, 986
Lsp1109I GCAGC 6 cut(s) 105, 183, 193, 233, 731, 767
LweI GCATC 4 cut(s) 193, 1039, 1156, 1372
MaeI CTAG 1 cut(s) 540
MaeII ACGT 2 cut(s) 279, 1107
MaeIII GTNAC 4 cut(s) 784, 850, 1231, 1448
MalI GATC 7 cut(s) 210, 651, 678, 888, 917, 1278, 1422
MbiI CCGCTC 1 cut(s) 149
MboI GATC 7 cut(s) 208, 649, 676, 886, 915, 1276, 1420
MboII GAAGA 5 cut(s) 24, 39, 179, 671, 731
MflI RGATCY 2 cut(s) 886, 1420
MhlI GDGCHC 7 cut(s) 227, 291, 332, 469, 1206, 1410, 1543
MluCI AATT 7 cut(s) 523, 638, 689, 810, 1114, 1343, 1391
MlyI GAGTC 1 cut(s) 1451
MmeI TCCRAC 1 cut(s) 586
Mph1103I ATGCAT 2 cut(s) 1171, 1436
MseI TTAA 4 cut(s) 996, 1059, 1161, 1397
MspA1I CMGCKG 2 cut(s) 196, 722
MspR9I CCNGG 2 cut(s) 1123, 1533
Mva1269I GAATGC 1 cut(s) 1169
MvaI CCWGG 2 cut(s) 1123, 1533
MvnI CGCG 3 cut(s) 86, 97, 339
NdeI CATATG 1 cut(s) 400
NdeII GATC 7 cut(s) 208, 649, 676, 886, 915, 1276, 1420
NlaIV GGNNCC 1 cut(s) 226
NmeAIII GCCGAG 1 cut(s) 310
NmuCI GTSAC 2 cut(s) 850, 1231
NsiI ATGCAT 2 cut(s) 1171, 1436
NspI RCATGY 1 cut(s) 1434
PaeI GCATGC 1 cut(s) 1434
PasI CCCWGGG 1 cut(s) 1122
PauI GCGCGC 2 cut(s) 84, 95
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PctI GAATGC 1 cut(s) 1169
PfeI GAWTC 4 cut(s) 293, 580, 1032, 1549
PkrI GCNGC 8 cut(s) 120, 183, 198, 248, 266, 721, 757, 1309
PleI GAGTC 1 cut(s) 1450
PpsI GAGTC 1 cut(s) 1450
Psp124BI GAGCTC 2 cut(s) 469, 1543
Psp1406I AACGTT 1 cut(s) 1107
Psp6I CCWGG 2 cut(s) 1121, 1531
PspFI CCCAGC 4 cut(s) 192, 360, 596, 892
PspGI CCWGG 2 cut(s) 1121, 1531
PspN4I GGNNCC 1 cut(s) 226
PspPI GGNCC 2 cut(s) 1136, 1151
PsrI GAACNNNNNNTAC 2 cut(s) 1253, 1285
PstI CTGCAG 1 cut(s) 721
PstNI CAGNNNCTG 1 cut(s) 1366
PsuI RGATCY 2 cut(s) 886, 1420
PteI GCGCGC 2 cut(s) 84, 95
PvuII CAGCTG 2 cut(s) 196, 722
RsaI GTAC 2 cut(s) 1262, 1326
RsaNI GTAC 2 cut(s) 1261, 1325
SacI GAGCTC 2 cut(s) 469, 1543
SaqAI TTAA 4 cut(s) 996, 1059, 1161, 1397
SatI GCNGC 8 cut(s) 119, 182, 197, 247, 265, 720, 756, 1308
Sau3AI GATC 7 cut(s) 208, 649, 676, 886, 915, 1276, 1420
Sau96I GGNCC 2 cut(s) 1136, 1151
SchI GAGTC 1 cut(s) 1451
ScrFI CCNGG 2 cut(s) 1123, 1533
SduI GDGCHC 7 cut(s) 227, 291, 332, 469, 1206, 1410, 1543
SfaNI GCATC 4 cut(s) 193, 1039, 1156, 1372
SfcI CTRYAG 2 cut(s) 717, 1002
SinI GGWCC 1 cut(s) 1151
SmlI CTYRAG 3 cut(s) 23, 470, 584
SmoI CTYRAG 3 cut(s) 23, 470, 584
SphI GCATGC 1 cut(s) 1434
Sse9I AATT 7 cut(s) 523, 638, 689, 810, 1114, 1343, 1391
SsiI CCGC 8 cut(s) 8, 42, 75, 147, 174, 265, 664, 1307
SspI AATATT 1 cut(s) 880
SspMI CTAG 1 cut(s) 540
SstI GAGCTC 2 cut(s) 469, 1543
StyD4I CCNGG 2 cut(s) 1121, 1531
TaaI ACNGT 2 cut(s) 660, 1358
TaiI ACGT 2 cut(s) 282, 1110
TaqI TCGA 3 cut(s) 372, 802, 1404
TasI AATT 7 cut(s) 523, 638, 689, 810, 1114, 1343, 1391
TauI GCSGC 2 cut(s) 267, 1310
TfiI GAWTC 4 cut(s) 293, 580, 1032, 1549
Tru1I TTAA 4 cut(s) 996, 1059, 1161, 1397
Tru9I TTAA 4 cut(s) 996, 1059, 1161, 1397
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 2 cut(s) 850, 1231
TseI GCWGC 6 cut(s) 118, 181, 196, 246, 719, 755
Tsp45I GTSAC 2 cut(s) 850, 1231
TspDTI ATGAA 5 cut(s) 32, 951, 1304, 1327, 1404
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 1151
XagI CCTNNNNNAGG 1 cut(s) 1248
XapI RAATTY 3 cut(s) 523, 638, 1391
XbaI TCTAGA 1 cut(s) 539
XceI RCATGY 1 cut(s) 1434
XspI CTAG 1 cut(s) 540
Zsp2I ATGCAT 2 cut(s) 1171, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.