Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0016169

Physical Location & Seq

Genomic Coordinates Reverse (-)

3693790 .. 3704454

Gene Structure 10665 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1051700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3102 bp

ATGGCGAATTTCAATGGTGTCGTCGACGGGGATCAGCAATGGCTGCTCAATTGCTTGTCTGCTACTCTCGACCCTAGCCACGAGGTTCGCTCGTTCGCCGAAGCTTCGCTTAATCAGGCTTCTGTGCAACCAGGTTTTGGAACTGCGTTATCCAAGGTTGCTGCAAACAGGGAGCTCCAGTTAGGATTGCGCCAGATATCCTTTTGTTATCATGCTGCTGTCCTTCTTAAACTGTTTATTAAGAAACACTGGCATGAGGGAGAGGAAGCATTTGAACATCCTGCTGTTTCAAGTGACGAGAAGGTAGTTGTACGCAGACTTCTGTTGTTGTCATTGGATGACTCTCATAGGAAAATTTGTACAGCAGTTAGTATGGCTGTTGCATCTATAGCAGCTTATGATTGGCCGGAGGATTGGCCTGACTTATTACCGTACCTGCTGAAGTTGATTAGTGATCATAATAATATGAATGGAGTACATGGTGCTCTAAGATGCTTGGCTCTTCTCTCTGTTGACTTGGATGATACAGTGGTTCCGACATTAGTACCAGCCTTGTTCCCATGCTTGCTTAAAATTGTATCATCTCCTCAGGTGTATGACAAATATTTTCGCACAAAATCCCTTTCAATTGTTTATTCTTGTATCTCCATGTTAGGGGTGATGAGTGGTGTATATAAGACGGAGACCAGTGCATTAATAATGCCAATGATTAAGCCATGGATGGATCAATTCTCTACAATCCTAAATCATCCGGTGCAGTCTGAAGATCCTGATGATTGGAGCATAAGAACAGAGGTATTGAAGTGCTTGAATCAGTTTGTTCAAAATTTTCCTAGCCTTATTGAAAGTGAATTTATGATTATTGTAGGGCCTTTGTGGCAAACTTTTATGAGTTCTCTTGGAGTATATGTGCGGTCATCTATTGAAGGTACAGAAGATCCATATGATGATAGATATGACTCTGATGGTGCTGAGAAAAGCCTTGACTCCTTTGTCATCCAGTTATTTGAGTTTCTGTTGACCATCGTGGGTAGTGCGAAATTGATAACGGTCATTATGAATAATGTCAAAGAGTTGACGTACAACACCATCGGTTTTCTCCAAATAACAGAACAACAGGTTCATACTTGGTCAATGGATGCCAACCAATTTGTAGCTGATGAGGATGATGTTACCTATAGCTGTCGTGTTTCTGGTGCACTTTTGCTTGAAGAAGTGGTGAATTCATGCGGTACTGAAGGAATCTGTGCCATCATTGATGCTGCAAAAAGATGTTTTAGTGAGTCTCAAAGGGAAAAGGATGTTGGTTCTGCAATTTGGTGGAGAATAAGGGAGGCTGCTCTTTTCGCTTTGTCTTCTCTATCAGACCAGTTGCTTGAAGCAGAGGAGTCCGAACTTACAAGAGTTGGCTTAGGAAACCTTCTGGAGCAAGTAATCACTGAAGACAGTGGATTAGATGTGCATCAATATCCCTTTCTTTATTCTCGTATCTTTTCATCAGTCGCTAAATTCTCCTCCGTGATCAGCCATGGAGTCCTTGAGCACTTTCTCTTTGCTGCTATCAAAGCAATTGGCATAGATGTGCCGCCACCTGTCAAAGTAGGTGCCTGCAGGGCACTCTCCGAGCTCCTACCTGAAATGAACAAAGGAATAATTCAACCGCATTTGATGAGTTTGTTTTCATCACTTTCAGAACTTCTTACTCAGGCCTCCGATGAAACCTTGCACCTGGTACTCGAAACACTGCAAGAGGCAATTAAGGCAGGTTACGAATTATCAGTATCCATTGAGCCTGTAATCTCTCCTGTGGTCCTCAACATGTGGGCATCACATATTTCCGATCCTTTTATCAGTATTGATGCAATTGAGGTTCTGGAGGCACTCAAAAACGCTCCTGGTTGTATTCGTCCACTGGTTTCTCGAGTTTTACCATGTGTTTGGCCAGTTATTAATCAACCACAACAACAGCCTGATGGATTAGTCGCTGGATCAGTGGATCTGGTAACAATGTTATTGAAAAATGCTCCTACTGATGTGGTAAAAACAGTATATGATGCTTGTTTTGATGCTGTTATACAAATAGTCCTTCAAAGTGATGATCACAGTGAAATGCAGAATGCTACGGAATGTTTAGCTGCCTTTATATCTGGTGGAAGACAAGATGTGCTAGCTTGGGGTGGTGATTCTGGAAATACAGTTAGAAGATTGCTTGATGCAGCTTCAAGGCTTCTGGACCCTAATTTGGAAAGCTCGGGTTCTCTTTTTGTTGGGAGCTACATTCTACAACTCATATTGCATTTGCCGTCACAAATGGCACCCCATATTCGAGACCTAGTTGCTGCTCTTCTTAGGCGCATGCGATCTGCTAAAATTGCAGGATTGAGAAGCTCACTGCTACTCATTTTCGCTCGGTTGGTTCACTTGAGTGCTCCAAATGTTGGACAGTTTATTGATCTGCTGGTCACCATTCCAGCTGACGGCTATGATAACTCTTTTGTCTATTTAATGTCAGAATGGACAAAACAGCAAGGTGAGATCCAGGGTGCCTACCAAATTAAAGTCACCACTACTGCATTGGCTTTATTACTATCATCTAGGCATGCTGAATTAGCAAAAATTAACGTACAAGGACATTTGGTTCAGTCTGCTGGGATCACCACCCGCTCAAAAGCTAAATTAACTCCAGATCAGTGGACTGTGGTGCCACTCCCTGCAAAGATAATGGCGTTACTGGCAGATGCATTGGTTGAAATCCAAGAACAGGGAGCTGGTGATAATGAGGATAGCGACTGGGAAGAAGTTGAGGCGGAGGATGGGGAACTTGACAAGGATTTGATTTATTCAGCTGGTGTTACATCATTTGGCAGACCCTCACATGAACATCTTGAAGCAATAGCAAAAACATTTGACAAGGAGGACAGTTATGAAGATGATCAACTAAGTGCAGCCGATCCCCTTAACAAGATTAATCTGGCAAACTATCTTGCGGAGTTTTTCGTAAACTTTTCCCAGAGCGAAAGACAAATGTTCGATCATCTTTTCCAGAATCTGACGCAGGATCAAAGGAATGCCATTCAAGCGATACGAACTCACGGAGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005829 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008536 GO:0008565 GO:0012505 GO:0015031 GO:0015833 GO:0017016 GO:0017038 GO:0019899 GO:0022613 GO:0031267 GO:0031647 GO:0031967 GO:0031975 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0042254 GO:0042393 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050821 GO:0051020 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594

KEGG Pathways

Metabolic & Signaling

ko00240 ko00983 ko01100 map00240 map00983 map01100

Pfam Domains

Protein Families

IBN_N Xpo1

Protein Analysis

1034

Amino Acids

114.09

Weight (kDa)

4.75

Isoelectric Point (pI)

43.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	33 - 112	4.2e-10	Importin-beta N-terminal domain
HEAT_IPO9_c	PF25018	356 - 594	1.5e-12	Importin-9 central HEAT repeats
TPR_IPO11	PF25758	626 - 997	2e-14	Importin-11-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0016169)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26170
fragaria_vesca	FvH4_4g28521 FvH4_4g28521
malus_domestica	MD16G1051700.v1.1
prunus_persica	Prupe.1G300500_v2.0.a1
pyrus_communis	pycom16g04540
rosa_chinensis	RchiOBHm_Chr4g0436541
rosa_laevigata	RLG00000006510
rosa_roxburghii	Rroxscaffold_5G00377650
rosa_rugosa	Rorug04G0294700
rosa_samantha	Rh4AG349000 Rh4BG357600 Rh4CG372000 Rh4DG351600
rosa_wichuraiana	Rw4G030530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	992
Acc36I	ACCTGC	2 cut(s)	444, 1754
AccB1I	GGYRCC	4 cut(s)	1604, 2314, 2543, 2701
AccB7I	CCANNNNNTGG	2 cut(s)	137, 2438
AccBSI	CCGCTC	1 cut(s)	2664
AccI	GTMKAC	1 cut(s)	24
AciI	CCGC	7 cut(s)	913, 1230, 1586, 1661, 2662, 2807, 2987
AcoI	YGGCCR	2 cut(s)	404, 1940
AcsI	RAATTY	6 cut(s)	7, 354, 826, 851, 1222, 1508
AcuI	CTGAAG	4 cut(s)	461, 783, 1257, 1461
AfiI	CCNNNNNNNGG	6 cut(s)	137, 654, 2242, 2438, 2477, 2761
AflIII	ACRYGT	1 cut(s)	1818
AjnI	CCWGG	4 cut(s)	130, 1728, 1894, 2538
AjuI	GAANNNNNNNTTGG	2 cut(s)	1287, 1319
AleI	CACNNNNGTG	1 cut(s)	2424
AloI	GAACNNNNNNTCC	2 cut(s)	2622, 2654
Alw21I	GWGCWC	6 cut(s)	177, 487, 1201, 1545, 1629, 2431
Alw26I	GTCTC	3 cut(s)	677, 1290, 2322
Alw44I	GTGCAC	1 cut(s)	1197
AlwNI	CAGNNNCTG	1 cut(s)	3049
Ama87I	CYCGRG	2 cut(s)	1920, 2251
AoxI	GGCC	5 cut(s)	404, 416, 869, 1707, 1940
ApaLI	GTGCAC	1 cut(s)	1197
ApoI	RAATTY	6 cut(s)	7, 354, 826, 851, 1222, 1508
AseI	ATTAAT	3 cut(s)	695, 1950, 2967
AspLEI	GCGC	2 cut(s)	192, 2355
AspS9I	GGNCC	3 cut(s)	869, 1810, 2233
AsuHPI	GGTGA	8 cut(s)	670, 1231, 2192, 2455, 2543, 2554, 2647, 2783
AsuNHI	GCTAGC	1 cut(s)	2167
AvaI	CYCGRG	2 cut(s)	1920, 2251
AvaII	GGWCC	2 cut(s)	1810, 2233
AxyI	CCTNAGG	1 cut(s)	588
BaeGI	GKGCMC	2 cut(s)	1201, 1618
BaeI	ACNNNNGTAYC	4 cut(s)	516, 528, 549, 561
BalI	TGGCCA	1 cut(s)	1942
BanI	GGYRCC	4 cut(s)	1604, 2314, 2543, 2701
BanII	GRGCYC	2 cut(s)	177, 1629
BarI	GAAGNNNNNNTAC	2 cut(s)	303, 335
BauI	CACGAG	1 cut(s)	80
BbsI	GAAGAC	3 cut(s)	1347, 1449, 2161
Bbv12I	GWGCWC	6 cut(s)	177, 487, 1201, 1545, 1629, 2431
BccI	CCATC	7 cut(s)	715, 959, 1031, 1097, 1259, 1967, 2807
BceAI	ACGGC	2 cut(s)	2287, 2494
BciT130I	CCWGG	4 cut(s)	132, 1730, 1896, 2540
BciVI	GTATCC	1 cut(s)	1792
BclI	TGATCA	4 cut(s)	454, 1521, 2098, 2932
BcoDI	GTCTC	3 cut(s)	677, 1290, 2322
BfaI	CTAG	5 cut(s)	75, 834, 2168, 2333, 2595
BfmI	CTRYAG	3 cut(s)	387, 1177, 1609
BfuAI	ACCTGC	2 cut(s)	444, 1754
BfuI	GTATCC	1 cut(s)	1792
BglI	GCCNNNNNGGC	2 cut(s)	877, 1613
Bme1390I	CCNGG	4 cut(s)	132, 1730, 1896, 2540
Bme18I	GGWCC	2 cut(s)	1810, 2233
BmeT110I	CYCGRG	2 cut(s)	1920, 2251
BmgT120I	GGNCC	3 cut(s)	869, 1810, 2233
BmiI	GGNNCC	6 cut(s)	534, 1606, 2235, 2316, 2545, 2703
BmrFI	CCNGG	4 cut(s)	132, 1730, 1896, 2540
BmrI	ACTGGG	1 cut(s)	2800
BmtI	GCTAGC	1 cut(s)	2171
BmuI	ACTGGG	1 cut(s)	2800
BpiI	GAAGAC	3 cut(s)	1347, 1449, 2161
BplI	GAGNNNNNCTC	2 cut(s)	74, 106
BpmI	CTGGAG	4 cut(s)	161, 1445, 1895, 2667
Bpu10I	CCTNAGC	1 cut(s)	1411
BpuEI	CTTGAG	2 cut(s)	1559, 2443
BsaBI	GATNNNNATC	1 cut(s)	1461
BsaI	GGTCTC	2 cut(s)	677, 2322
BsaJI	CCNNGG	4 cut(s)	153, 716, 1528, 2539
BsaWI	WCCGGW	1 cut(s)	751
BsaXI	ACNNNNNCTCC	4 cut(s)	164, 194, 894, 924
Bsc4I	CCNNNNNNNGG	6 cut(s)	137, 654, 2242, 2438, 2477, 2761
Bse1I	ACTGG	9 cut(s)	178, 254, 687, 1000, 1369, 1917, 1943, 2736, 2795
Bse21I	CCTNAGG	1 cut(s)	588
Bse3DI	GCAATG	1 cut(s)	44
Bse8I	GATNNNNATC	1 cut(s)	1461
BseBI	CCWGG	4 cut(s)	132, 1730, 1896, 2540
BseDI	CCNNGG	4 cut(s)	153, 716, 1528, 2539
BseJI	GATNNNNATC	1 cut(s)	1461
BseLI	CCNNNNNNNGG	6 cut(s)	137, 654, 2242, 2438, 2477, 2761
BseMI	GCAATG	1 cut(s)	44
BseMII	CTCAG	3 cut(s)	602, 963, 1718
BseNI	ACTGG	9 cut(s)	178, 254, 687, 1000, 1369, 1917, 1943, 2736, 2795
BseRI	GAGGAG	3 cut(s)	576, 1400, 1504
BseSI	GKGCMC	2 cut(s)	1201, 1618
BseYI	CCCAGC	1 cut(s)	2648
BsgI	GTGCAG	2 cut(s)	776, 2964
BshFI	GGCC	5 cut(s)	406, 418, 871, 1709, 1942
BshNI	GGYRCC	4 cut(s)	1604, 2314, 2543, 2701
BsiHKAI	GWGCWC	6 cut(s)	177, 487, 1201, 1545, 1629, 2431
BsiHKCI	CYCGRG	2 cut(s)	1920, 2251
BsiSI	CCGG	2 cut(s)	407, 752
BslI	CCNNNNNNNGG	6 cut(s)	137, 654, 2242, 2438, 2477, 2761
BsmAI	GTCTC	3 cut(s)	677, 1290, 2322
BsmI	GAATGC	2 cut(s)	2122, 3073
BsnI	GGCC	5 cut(s)	406, 418, 871, 1709, 1942
Bso31I	GGTCTC	2 cut(s)	677, 2322
BsoBI	CYCGRG	2 cut(s)	1920, 2251
Bsp1286I	GDGCHC	7 cut(s)	177, 487, 1201, 1545, 1618, 1629, 2431
Bsp1407I	TGTACA	1 cut(s)	359
Bsp19I	CCATGG	2 cut(s)	716, 1528
BspACI	CCGC	7 cut(s)	913, 1230, 1586, 1661, 2662, 2807, 2987
BspANI	GGCC	5 cut(s)	406, 418, 871, 1709, 1942
BspCNI	CTCAG	3 cut(s)	601, 964, 1717
BspLI	GGNNCC	6 cut(s)	534, 1606, 2235, 2316, 2545, 2703
BspMAI	CTGCAG	1 cut(s)	1613
BspMI	ACCTGC	2 cut(s)	444, 1754
BspOI	GCTAGC	1 cut(s)	2171
BspQI	GCTCTTC	2 cut(s)	507, 2349
BspT107I	GGYRCC	4 cut(s)	1604, 2314, 2543, 2701
BspTNI	GGTCTC	2 cut(s)	677, 2322
BsrBI	CCGCTC	1 cut(s)	2664
BsrDI	GCAATG	1 cut(s)	44
BsrGI	TGTACA	1 cut(s)	359
BsrI	ACTGG	9 cut(s)	178, 254, 687, 1000, 1369, 1917, 1943, 2736, 2795
BssECI	CCNNGG	4 cut(s)	153, 716, 1528, 2539
BssSI	CACGAG	1 cut(s)	80
BssT1I	CCWWGG	3 cut(s)	153, 716, 1528
Bst2BI	CACGAG	1 cut(s)	80
Bst2UI	CCWGG	4 cut(s)	132, 1730, 1896, 2540
Bst6I	CTCTTC	2 cut(s)	507, 2349
BstAPI	GCANNNNNTGC	1 cut(s)	43
BstAUI	TGTACA	1 cut(s)	359
BstC8I	GCNNGC	5 cut(s)	566, 1609, 2169, 2357, 2601
BstDEI	CTNAG	7 cut(s)	488, 588, 972, 1411, 1704, 2348, 2939
BstDSI	CCRYGG	2 cut(s)	716, 1528
BstEII	GGTNACC	1 cut(s)	2461
BstHHI	GCGC	2 cut(s)	192, 2355
BstMAI	GTCTC	3 cut(s)	677, 1290, 2322
BstNI	CCWGG	4 cut(s)	132, 1730, 1896, 2540
BstNSI	RCATGY	3 cut(s)	1822, 2359, 2603
BstPI	GGTNACC	1 cut(s)	2461
BstSCI	CCNGG	4 cut(s)	130, 1728, 1894, 2538
BstSFI	CTRYAG	3 cut(s)	387, 1177, 1609
BstSLI	GKGCMC	2 cut(s)	1201, 1618
BstV2I	GAAGAC	3 cut(s)	1347, 1449, 2161
BstX2I	RGATCY	4 cut(s)	766, 937, 1996, 2535
BstXI	CCANNNNNNTGG	2 cut(s)	1938, 2691
BstYI	RGATCY	4 cut(s)	766, 937, 1996, 2535
Bsu36I	CCTNAGG	1 cut(s)	588
BsuI	GTATCC	1 cut(s)	1792
BsuRI	GGCC	5 cut(s)	406, 418, 871, 1709, 1942
BtgI	CCRYGG	2 cut(s)	716, 1528
BtsI	GCAGTG	2 cut(s)	1742, 2390
BveI	ACCTGC	2 cut(s)	444, 1754
Cac8I	GCNNGC	5 cut(s)	566, 1609, 2169, 2357, 2601
CaiI	CAGNNNCTG	1 cut(s)	3049
CfoI	GCGC	2 cut(s)	192, 2355
Cfr13I	GGNCC	3 cut(s)	869, 1810, 2233
CseI	GACGC	1 cut(s)	3061
CsiI	ACCWGGT	2 cut(s)	130, 1728
DdeI	CTNAG	7 cut(s)	488, 588, 972, 1411, 1704, 2348, 2939
DrdI	GACNNNNNNGTC	1 cut(s)	992
DseDI	GACNNNNNNGTC	1 cut(s)	992
EaeI	YGGCCR	2 cut(s)	404, 1940
Eam1104I	CTCTTC	2 cut(s)	507, 2349
EarI	CTCTTC	2 cut(s)	507, 2349
EciI	GGCGGA	1 cut(s)	2822
Ecl136II	GAGCTC	2 cut(s)	175, 1627
Eco130I	CCWWGG	3 cut(s)	153, 716, 1528
Eco147I	AGGCCT	1 cut(s)	1709
Eco24I	GRGCYC	2 cut(s)	177, 1629
Eco31I	GGTCTC	2 cut(s)	677, 2322
Eco32I	GATATC	1 cut(s)	198
Eco47I	GGWCC	2 cut(s)	1810, 2233
Eco53kI	GAGCTC	2 cut(s)	175, 1627
Eco57I	CTGAAG	4 cut(s)	461, 783, 1257, 1461
Eco81I	CCTNAGG	1 cut(s)	588
Eco88I	CYCGRG	2 cut(s)	1920, 2251
Eco91I	GGTNACC	1 cut(s)	2461
EcoICRI	GAGCTC	2 cut(s)	175, 1627
EcoO109I	RGGNCCY	1 cut(s)	869
EcoO65I	GGTNACC	1 cut(s)	2461
EcoRI	GAATTC	1 cut(s)	1222
EcoRII	CCWGG	4 cut(s)	130, 1728, 1894, 2538
EcoRV	GATATC	1 cut(s)	198
EcoT14I	CCWWGG	3 cut(s)	153, 716, 1528
EcoT22I	ATGCAT	1 cut(s)	2743
EcoT38I	GRGCYC	2 cut(s)	177, 1629
ErhI	CCWWGG	3 cut(s)	153, 716, 1528
FalI	AAGNNNNNCTT	2 cut(s)	93, 125
FauI	CCCGC	1 cut(s)	2669
FauNDI	CATATG	1 cut(s)	943
FbaI	TGATCA	4 cut(s)	454, 1521, 2098, 2932
FblI	GTMKAC	1 cut(s)	24
FriOI	GRGCYC	2 cut(s)	177, 1629
FspBI	CTAG	5 cut(s)	75, 834, 2168, 2333, 2595
GlaI	GCGC	2 cut(s)	191, 2354
GsaI	CCCAGC	1 cut(s)	2652
GsuI	CTGGAG	4 cut(s)	161, 1445, 1895, 2667
HaeIII	GGCC	5 cut(s)	406, 418, 871, 1709, 1942
HapII	CCGG	2 cut(s)	407, 752
HgaI	GACGC	1 cut(s)	3061
HhaI	GCGC	2 cut(s)	192, 2355
Hin6I	GCGC	2 cut(s)	190, 2353
HinP1I	GCGC	2 cut(s)	190, 2353
HincII	GTYRAC	4 cut(s)	25, 514, 1020, 1077
HindII	GTYRAC	4 cut(s)	25, 514, 1020, 1077
HindIII	AAGCTT	1 cut(s)	102
HpaII	CCGG	2 cut(s)	407, 752
HphI	GGTGA	8 cut(s)	670, 1231, 2192, 2455, 2543, 2554, 2647, 2783
Hpy166II	GTNNAC	9 cut(s)	25, 514, 1020, 1077, 1199, 1910, 2419, 2694, 3001
Hpy8I	GTNNAC	9 cut(s)	25, 514, 1020, 1077, 1199, 1910, 2419, 2694, 3001
Hpy99I	CGWCG	2 cut(s)	26, 29
HpyAV	CCTTC	6 cut(s)	233, 295, 920, 1232, 1430, 2096
HpyCH4IV	ACGT	2 cut(s)	1079, 2622
HpyF3I	CTNAG	7 cut(s)	488, 588, 972, 1411, 1704, 2348, 2939
HpySE526I	ACGT	2 cut(s)	1079, 2622
HspAI	GCGC	2 cut(s)	190, 2353
Ksp22I	TGATCA	4 cut(s)	454, 1521, 2098, 2932
LguI	GCTCTTC	2 cut(s)	507, 2349
MabI	ACCWGGT	2 cut(s)	130, 1728
MaeI	CTAG	5 cut(s)	75, 834, 2168, 2333, 2595
MaeII	ACGT	2 cut(s)	1079, 2622
MaeIII	GTNAC	9 cut(s)	293, 1171, 1766, 2002, 2304, 2461, 2560, 2727, 2851
MbiI	CCGCTC	1 cut(s)	2664
MfeI	CAATTG	4 cut(s)	49, 627, 1569, 1863
MflI	RGATCY	4 cut(s)	766, 937, 1996, 2535
MhlI	GDGCHC	7 cut(s)	177, 487, 1201, 1545, 1618, 1629, 2431
MlsI	TGGCCA	1 cut(s)	1942
MluNI	TGGCCA	1 cut(s)	1942
MlyI	GAGTC	6 cut(s)	335, 953, 980, 1292, 1397, 1542
MmeI	TCCRAC	2 cut(s)	560, 2419
Mox20I	TGGCCA	1 cut(s)	1942
Mph1103I	ATGCAT	1 cut(s)	2743
MscI	TGGCCA	1 cut(s)	1942
MslI	CAYNNNNRTG	3 cut(s)	252, 1580, 2424
Msp20I	TGGCCA	1 cut(s)	1942
MspA1I	CMGCKG	2 cut(s)	2474, 2846
MspI	CCGG	2 cut(s)	407, 752
MspR9I	CCNGG	4 cut(s)	132, 1730, 1896, 2540
MunI	CAATTG	4 cut(s)	49, 627, 1569, 1863
Mva1269I	GAATGC	2 cut(s)	2122, 3073
MvaI	CCWGG	4 cut(s)	132, 1730, 1896, 2540
NcoI	CCATGG	2 cut(s)	716, 1528
NdeI	CATATG	1 cut(s)	943
NheI	GCTAGC	1 cut(s)	2167
NlaIV	GGNNCC	6 cut(s)	534, 1606, 2235, 2316, 2545, 2703
NmuCI	GTSAC	4 cut(s)	293, 2304, 2461, 2560
NsiI	ATGCAT	1 cut(s)	2743
NspI	RCATGY	3 cut(s)	1822, 2359, 2603
OliI	CACNNNNGTG	1 cut(s)	2424
PaeI	GCATGC	2 cut(s)	2359, 2603
PaeR7I	CTCGAG	1 cut(s)	1920
PceI	AGGCCT	1 cut(s)	1709
PciI	ACATGT	1 cut(s)	1818
PciSI	GCTCTTC	2 cut(s)	507, 2349
PctI	GAATGC	2 cut(s)	2122, 3073
PfeI	GAWTC	4 cut(s)	811, 1242, 2183, 3046
PflMI	CCANNNNNTGG	2 cut(s)	137, 2438
PleI	GAGTC	6 cut(s)	335, 953, 980, 1291, 1396, 1541
PpsI	GAGTC	6 cut(s)	335, 953, 980, 1291, 1396, 1541
PscI	ACATGT	1 cut(s)	1818
PshBI	ATTAAT	3 cut(s)	695, 1950, 2967
Psp124BI	GAGCTC	2 cut(s)	177, 1629
Psp6I	CCWGG	4 cut(s)	130, 1728, 1894, 2538
PspEI	GGTNACC	1 cut(s)	2461
PspFI	CCCAGC	1 cut(s)	2648
PspGI	CCWGG	4 cut(s)	130, 1728, 1894, 2538
PspN4I	GGNNCC	6 cut(s)	534, 1606, 2235, 2316, 2545, 2703
PspPI	GGNCC	3 cut(s)	869, 1810, 2233
PstI	CTGCAG	1 cut(s)	1613
PstNI	CAGNNNCTG	1 cut(s)	3049
PsuI	RGATCY	4 cut(s)	766, 937, 1996, 2535
PvuII	CAGCTG	2 cut(s)	2474, 2846
RseI	CAYNNNNRTG	3 cut(s)	252, 1580, 2424
SacI	GAGCTC	2 cut(s)	177, 1629
SalI	GTCGAC	1 cut(s)	23
SapI	GCTCTTC	2 cut(s)	507, 2349
Sau96I	GGNCC	3 cut(s)	869, 1810, 2233
SbfI	CCTGCAGG	1 cut(s)	1613
SchI	GAGTC	6 cut(s)	335, 953, 980, 1292, 1397, 1542
ScrFI	CCNGG	4 cut(s)	132, 1730, 1896, 2540
SdaI	CCTGCAGG	1 cut(s)	1613
SduI	GDGCHC	7 cut(s)	177, 487, 1201, 1545, 1618, 1629, 2431
SexAI	ACCWGGT	2 cut(s)	130, 1728
SfcI	CTRYAG	3 cut(s)	387, 1177, 1609
Sfr274I	CTCGAG	1 cut(s)	1920
SgrDI	CGTCGACG	1 cut(s)	23
SinI	GGWCC	2 cut(s)	1810, 2233
SlaI	CTCGAG	1 cut(s)	1920
SmiMI	CAYNNNNRTG	3 cut(s)	252, 1580, 2424
SmlI	CTYRAG	3 cut(s)	1538, 1920, 2422
SmoI	CTYRAG	3 cut(s)	1538, 1920, 2422
SphI	GCATGC	2 cut(s)	2359, 2603
Sse8387I	CCTGCAGG	1 cut(s)	1613
SseBI	AGGCCT	1 cut(s)	1709
SsiI	CCGC	7 cut(s)	913, 1230, 1586, 1661, 2662, 2807, 2987
SspI	AATATT	1 cut(s)	605
SspMI	CTAG	5 cut(s)	75, 834, 2168, 2333, 2595
SstI	GAGCTC	2 cut(s)	177, 1629
StuI	AGGCCT	1 cut(s)	1709
StyD4I	CCNGG	4 cut(s)	130, 1728, 1894, 2538
StyI	CCWWGG	3 cut(s)	153, 716, 1528
TaiI	ACGT	2 cut(s)	1082, 2625
TaqI	TCGA	6 cut(s)	24, 69, 1737, 1921, 2326, 3030
TatI	WGTACW	2 cut(s)	359, 475
TauI	GCSGC	1 cut(s)	1588
TfiI	GAWTC	4 cut(s)	811, 1242, 2183, 3046
TseFI	GTSAC	4 cut(s)	293, 2304, 2461, 2560
Tsp45I	GTSAC	4 cut(s)	293, 2304, 2461, 2560
TspGWI	ACGGA	3 cut(s)	695, 1507, 2138
Van91I	CCANNNNNTGG	2 cut(s)	137, 2438
VneI	GTGCAC	1 cut(s)	1197
VpaK11BI	GGWCC	2 cut(s)	1810, 2233
VspI	ATTAAT	3 cut(s)	695, 1950, 2967
XapI	RAATTY	6 cut(s)	7, 354, 826, 851, 1222, 1508
XceI	RCATGY	3 cut(s)	1822, 2359, 2603
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2572
XhoI	CTCGAG	1 cut(s)	1920
XmiI	GTMKAC	1 cut(s)	24
XspI	CTAG	5 cut(s)	75, 834, 2168, 2333, 2595
Zsp2I	ATGCAT	1 cut(s)	2743

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.