Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010692

Physical Location & Seq

Genomic Coordinates Forward (+)

4739270 .. 4747108

Gene Structure 7839 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1067300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 669 bp

ATGAGTGAGGTATTCGAAGGCTACGAGCGTCAGTACTGCGAGCTCTCCGCAAACCTCTCGCGAAAATCCAACTCCGCCGCCCTTCTTCCCGACTATGAGCAGAAGAAGCAGAAATTTTCTGAGATTAAAATTGGACTGGATGATGCCGAAGCTTTGATTCGGAAAATGGACCTCGAAGCCAGAAGCTTGCAGCCGAGCGTGAAGGCGGTGCTTCTTGCTAAGCTAAGGGAGTATAAATCTGATCTTAATAAGTTGAAAAGGGAAATCAAAAGAGTGGCATCGCCTGATGCCAGTCAGGCTGCTCGGGACGAACTACTGGAGGCAGGAATGGCTGATGCGCATGCGGTTTCTTCTGATCAAAGAGAGAGAATGACAATGTCTGTTGAGAGATTGAATGAATCAAGTGATAGAATCACACAGAGTAGAAGAACGATATTGGAAACCGAAGAGCTTGGTGTTTCCATTCTCCAAGATTTGCATCAACAGCGGGAAACTCTCCTGCATTCCCATCAAAAACTTAATGCAGTAGATGACGCCATTGACAAGAGTAAAAGAGTTTTAACTGCCATGTCGCGGAGGATGACAAAGCATAAATGGATCATCGGCTCAGTTATCGGAGCTCTTATCGTTGCAATCCTCTTTATCCTATACTTTAAGCTTTCTCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005483 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005783 GO:0005789 GO:0005794 GO:0005798 GO:0005802 GO:0005886 GO:0006605 GO:0006623 GO:0006810 GO:0006886 GO:0006888 GO:0006892 GO:0006896 GO:0006906 GO:0006996 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0010008 GO:0012505 GO:0012506 GO:0012507 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016197 GO:0016482 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031201 GO:0031410 GO:0031902 GO:0031982 GO:0031984 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042147 GO:0042175 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048280 GO:0048284 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061024 GO:0061025 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072594 GO:0072665 GO:0072666 GO:0090174 GO:0097708 GO:0098588 GO:0098791 GO:0098796 GO:0098805 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko04130 ko04138 map04130 map04138

Pfam Domains

Protein Families

V-SNARE V-SNARE_C

Protein Analysis

223

Amino Acids

25.3

Weight (kDa)

8.87

Isoelectric Point (pI)

52.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
V-SNARE	PF05008	1 - 92	2.5e-29	Vesicle transport v-SNARE protein N-terminus
V-SNARE_C	PF12352	129 - 194	6.4e-21	Snare region anchored in the vesicle membrane C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0010692)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26670
fragaria_vesca	FvH4_4g30751 FvH4_4g30751
malus_domestica	MD13G1068500.v1.1 MD16G1067300.v1.1
prunus_persica	Prupe.1G279400_v2.0.a1
pyrus_communis	pycom13g06030 pycom16g05880
rosa_chinensis	RchiOBHm_Chr4g0439151
rosa_laevigata	RLG00000006293
rosa_multiflora	Rmu_sc0002236.1_g000003 Rmu_sc0027506.1_g000004
rosa_rugosa	Rorug04G0316100 Rorug04G0316200
rosa_samantha	Rh4AG367000 Rh4BG378500 Rh4CG392800 Rh4DG373100
rosa_wichuraiana	Rw0G007520 Rw0G011710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	339
AccII	CGCG	2 cut(s)	61, 574
AciI	CCGC	7 cut(s)	48, 75, 78, 206, 344, 487, 574
AclWI	GGATC	1 cut(s)	605
AcsI	RAATTY	1 cut(s)	113
AcyI	GRCGYC	1 cut(s)	534
AfaI	GTAC	1 cut(s)	35
AfiI	CCNNNNNNNGG	1 cut(s)	573
AgsI	TTSAA	2 cut(s)	256, 394
AluBI	AGCT	7 cut(s)	43, 152, 186, 223, 451, 620, 658
AluI	AGCT	7 cut(s)	43, 152, 186, 223, 451, 620, 658
Alw21I	GWGCWC	2 cut(s)	45, 622
AlwI	GGATC	1 cut(s)	605
Ama87I	CYCGRG	1 cut(s)	303
ApeKI	GCWGC	2 cut(s)	190, 299
ApoI	RAATTY	1 cut(s)	113
AspLEI	GCGC	1 cut(s)	340
AspS9I	GGNCC	1 cut(s)	169
AsuII	TTCGAA	1 cut(s)	15
AvaI	CYCGRG	1 cut(s)	303
AvaII	GGWCC	1 cut(s)	169
BanII	GRGCYC	2 cut(s)	45, 622
Bbv12I	GWGCWC	2 cut(s)	45, 622
BbvI	GCAGC	2 cut(s)	202, 286
BccI	CCATC	1 cut(s)	516
BcgI	CGANNNNNNTGC	2 cut(s)	39, 73
BclI	TGATCA	1 cut(s)	355
BglI	GCCNNNNNGGC	1 cut(s)	296
BisI	GCNGC	3 cut(s)	78, 191, 300
BlpI	GCTNAGC	1 cut(s)	219
BlsI	GCNGC	3 cut(s)	79, 192, 301
BmcAI	AGTACT	1 cut(s)	35
Bme18I	GGWCC	1 cut(s)	169
BmeT110I	CYCGRG	1 cut(s)	303
BmgT120I	GGNCC	1 cut(s)	169
BmsI	GCATC	5 cut(s)	133, 277, 287, 325, 487
BpmI	CTGGAG	1 cut(s)	338
Bpu10I	CCTNAGC	1 cut(s)	224
Bpu1102I	GCTNAGC	1 cut(s)	219
Bpu14I	TTCGAA	1 cut(s)	15
BsaBI	GATNNNNATC	1 cut(s)	477
BsaHI	GRCGYC	1 cut(s)	534
Bsc4I	CCNNNNNNNGG	1 cut(s)	573
Bse1I	ACTGG	3 cut(s)	141, 291, 321
Bse8I	GATNNNNATC	1 cut(s)	477
BseGI	GGATG	2 cut(s)	145, 585
BseJI	GATNNNNATC	1 cut(s)	477
BseLI	CCNNNNNNNGG	1 cut(s)	573
BseMII	CTCAG	2 cut(s)	111, 621
BseNI	ACTGG	3 cut(s)	141, 291, 321
BseXI	GCAGC	2 cut(s)	202, 286
Bsh1236I	CGCG	2 cut(s)	61, 574
BsiHKAI	GWGCWC	2 cut(s)	45, 622
BsiHKCI	CYCGRG	1 cut(s)	303
BslFI	GGGAC	1 cut(s)	320
BslI	CCNNNNNNNGG	1 cut(s)	573
BsmFI	GGGAC	1 cut(s)	320
BsmI	GAATGC	1 cut(s)	502
BsoBI	CYCGRG	1 cut(s)	303
Bsp119I	TTCGAA	1 cut(s)	15
Bsp1286I	GDGCHC	2 cut(s)	45, 622
Bsp143I	GATC	3 cut(s)	241, 355, 597
Bsp1720I	GCTNAGC	1 cut(s)	219
Bsp68I	TCGCGA	1 cut(s)	61
BspACI	CCGC	7 cut(s)	48, 75, 78, 206, 344, 487, 574
BspCNI	CTCAG	2 cut(s)	112, 620
BspFNI	CGCG	2 cut(s)	61, 574
BspPI	GGATC	1 cut(s)	605
BspQI	GCTCTTC	1 cut(s)	441
BspT104I	TTCGAA	1 cut(s)	15
BsrI	ACTGG	3 cut(s)	141, 291, 321
BssMI	GATC	3 cut(s)	241, 355, 597
BssNI	GRCGYC	1 cut(s)	534
Bst6I	CTCTTC	1 cut(s)	441
BstACI	GRCGYC	1 cut(s)	534
BstBI	TTCGAA	1 cut(s)	15
BstC8I	GCNNGC	3 cut(s)	41, 188, 342
BstDEI	CTNAG	4 cut(s)	120, 219, 224, 607
BstF5I	GGATG	2 cut(s)	145, 585
BstFNI	CGCG	2 cut(s)	61, 574
BstHHI	GCGC	1 cut(s)	340
BstKTI	GATC	3 cut(s)	244, 358, 600
BstMBI	GATC	3 cut(s)	241, 355, 597
BstMWI	GCNNNNNNNGC	4 cut(s)	106, 296, 329, 484
BstNSI	RCATGY	1 cut(s)	344
BstUI	CGCG	2 cut(s)	61, 574
BstV1I	GCAGC	2 cut(s)	202, 286
BtgZI	GCGATG	1 cut(s)	264
BtsCI	GGATG	2 cut(s)	145, 585
BtuMI	TCGCGA	1 cut(s)	61
Cac8I	GCNNGC	3 cut(s)	41, 188, 342
CfoI	GCGC	1 cut(s)	340
Cfr13I	GGNCC	1 cut(s)	169
CseI	GACGC	2 cut(s)	17, 542
Csp6I	GTAC	1 cut(s)	34
CviAII	CATG	2 cut(s)	341, 568
CviQI	GTAC	1 cut(s)	34
DdeI	CTNAG	4 cut(s)	120, 219, 224, 607
DpnI	GATC	3 cut(s)	243, 357, 599
DpnII	GATC	3 cut(s)	241, 355, 597
Eam1104I	CTCTTC	1 cut(s)	441
EarI	CTCTTC	1 cut(s)	441
EciI	GGCGGA	1 cut(s)	64
Ecl136II	GAGCTC	2 cut(s)	43, 620
Eco24I	GRGCYC	2 cut(s)	45, 622
Eco47I	GGWCC	1 cut(s)	169
Eco53kI	GAGCTC	2 cut(s)	43, 620
Eco88I	CYCGRG	1 cut(s)	303
EcoICRI	GAGCTC	2 cut(s)	43, 620
EcoT38I	GRGCYC	2 cut(s)	45, 622
FaeI	CATG	2 cut(s)	344, 571
FaiI	YATR	6 cut(s)	96, 234, 342, 569, 591, 649
FaqI	GGGAC	1 cut(s)	320
FatI	CATG	2 cut(s)	340, 567
FauI	CCCGC	1 cut(s)	480
FbaI	TGATCA	1 cut(s)	355
Fnu4HI	GCNGC	3 cut(s)	78, 191, 300
FokI	GGATG	2 cut(s)	152, 592
FriOI	GRGCYC	2 cut(s)	45, 622
Fsp4HI	GCNGC	3 cut(s)	78, 191, 300
FspAI	RTGCGCAY	1 cut(s)	339
FspI	TGCGCA	1 cut(s)	339
GlaI	GCGC	1 cut(s)	339
GluI	GCNGC	3 cut(s)	78, 191, 300
GsuI	CTGGAG	1 cut(s)	338
HgaI	GACGC	2 cut(s)	17, 542
HhaI	GCGC	1 cut(s)	340
Hin1I	GRCGYC	1 cut(s)	534
Hin1II	CATG	2 cut(s)	344, 571
Hin6I	GCGC	1 cut(s)	338
HinP1I	GCGC	1 cut(s)	338
HindIII	AAGCTT	3 cut(s)	150, 184, 656
HinfI	GANTC	3 cut(s)	157, 398, 411
Hpy188I	TCNGA	5 cut(s)	121, 162, 241, 355, 617
Hpy188III	TCNNGA	3 cut(s)	60, 89, 305
HpyAV	CCTTC	3 cut(s)	11, 92, 196
HpyCH4V	TGCA	5 cut(s)	190, 478, 502, 524, 632
HpyF10VI	GCNNNNNNNGC	4 cut(s)	106, 296, 329, 484
HpyF3I	CTNAG	4 cut(s)	120, 219, 224, 607
Hsp92I	GRCGYC	1 cut(s)	534
Hsp92II	CATG	2 cut(s)	344, 571
HspAI	GCGC	1 cut(s)	338
Ksp22I	TGATCA	1 cut(s)	355
Kzo9I	GATC	3 cut(s)	241, 355, 597
LguI	GCTCTTC	1 cut(s)	441
LmnI	GCTCC	1 cut(s)	617
LpnPI	CCDG	8 cut(s)	122, 193, 281, 297, 302, 304, 309, 512
Lsp1109I	GCAGC	2 cut(s)	202, 286
LweI	GCATC	5 cut(s)	133, 277, 287, 325, 487
MalI	GATC	3 cut(s)	243, 357, 599
MboI	GATC	3 cut(s)	241, 355, 597
MboII	GAAGA	5 cut(s)	77, 115, 342, 438, 458
MhlI	GDGCHC	2 cut(s)	45, 622
MluCI	AATT	2 cut(s)	113, 129
MmeI	TCCRAC	1 cut(s)	93
MnlI	CCTC	5 cut(s)	65, 182, 313, 570, 647
MseI	TTAA	6 cut(s)	126, 246, 519, 560, 654, 667
MspA1I	CMGCKG	1 cut(s)	487
Mva1269I	GAATGC	1 cut(s)	502
MvnI	CGCG	2 cut(s)	61, 574
MwoI	GCNNNNNNNGC	4 cut(s)	106, 296, 329, 484
NdeII	GATC	3 cut(s)	241, 355, 597
NlaIII	CATG	2 cut(s)	344, 571
NmeAIII	GCCGAG	1 cut(s)	219
NruI	TCGCGA	1 cut(s)	61
NsbI	TGCGCA	1 cut(s)	339
NspI	RCATGY	1 cut(s)	344
NspV	TTCGAA	1 cut(s)	15
PaeI	GCATGC	1 cut(s)	344
PciSI	GCTCTTC	1 cut(s)	441
PcsI	WCGNNNNNNNCGW	1 cut(s)	21
PctI	GAATGC	1 cut(s)	502
PfeI	GAWTC	3 cut(s)	157, 398, 411
PflFI	GACNNNGTC	1 cut(s)	376
PkrI	GCNGC	3 cut(s)	79, 192, 301
Psp124BI	GAGCTC	2 cut(s)	45, 622
PspPI	GGNCC	1 cut(s)	169
PsyI	GACNNNGTC	1 cut(s)	376
RruI	TCGCGA	1 cut(s)	61
RsaI	GTAC	1 cut(s)	35
RsaNI	GTAC	1 cut(s)	34
SacI	GAGCTC	2 cut(s)	45, 622
SapI	GCTCTTC	1 cut(s)	441
SaqAI	TTAA	6 cut(s)	126, 246, 519, 560, 654, 667
SatI	GCNGC	3 cut(s)	78, 191, 300
Sau3AI	GATC	3 cut(s)	241, 355, 597
Sau96I	GGNCC	1 cut(s)	169
ScaI	AGTACT	1 cut(s)	35
SduI	GDGCHC	2 cut(s)	45, 622
SfaNI	GCATC	5 cut(s)	133, 277, 287, 325, 487
SfuI	TTCGAA	1 cut(s)	15
SinI	GGWCC	1 cut(s)	169
SphI	GCATGC	1 cut(s)	344
Sse9I	AATT	2 cut(s)	113, 129
SsiI	CCGC	7 cut(s)	48, 75, 78, 206, 344, 487, 574
SstI	GAGCTC	2 cut(s)	45, 622
TaqI	TCGA	2 cut(s)	15, 174
TasI	AATT	2 cut(s)	113, 129
TatI	WGTACW	1 cut(s)	33
TauI	GCSGC	1 cut(s)	80
TfiI	GAWTC	3 cut(s)	157, 398, 411
Tru1I	TTAA	6 cut(s)	126, 246, 519, 560, 654, 667
Tru9I	TTAA	6 cut(s)	126, 246, 519, 560, 654, 667
TseI	GCWGC	2 cut(s)	190, 299
TspDTI	ATGAA	1 cut(s)	411
Tth111I	GACNNNGTC	1 cut(s)	376
VpaK11BI	GGWCC	1 cut(s)	169
XapI	RAATTY	1 cut(s)	113
XceI	RCATGY	1 cut(s)	344
ZrmI	AGTACT	1 cut(s)	35

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.