Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0012229

Physical Location & Seq

Genomic Coordinates Forward (+)

5696852 .. 5698829

Gene Structure 1978 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1080900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1977 bp

ATGACTGCTGAAACTTCACTCTTTCCCTTCCCACTCCCATTCCCTTCCACATCCATGCCTCCACCACCACTACCACCATACTCCGTCGCGCTCTCAGTCCTGATCCTCCTAACACTCTACTCAGCTGCCACCGCCGCCCACTCATGTTTCCCAAAACCATCGTCCTTCTCCACATTTTCATGTCCACCATTCACTTCACCGCCGCCGTTCCCCTTCTCTTCTTCACCAGGATGCGGCCACCCTTCTTTCCAAATCAAATGCTCTGCCCCCCACTCCACCATCTCCATCAATAACCTCTCTTTCTCTCTCATCAGCTACGATCCCATCGCCTCCTCGCTTCTCCTCTCTCCCCACACCACCAACTCAACAGTCACAAACTGCTCGTCCTCTAACTATCCTTCCATTCCTAGCCGCTCCATCAACATCTCCGCCACCCCATTTCGAATATCCGACGCCTCCTGCTCCCGCCTCTCAGTTCTCAAGCCTTGCTTCTCGCCAAATCTTCCCAACTGCAGCCATTGCCCTTGGGACTGCAAGCTCATCAAACACCCACTCAAGCTTCTCAACGACTGCGGATCTCCGCACCGCCCTGTCACCCAGCAGGGCTGCCACGACGACGTCTTGAGCTTTCTCGACGATTTCCTCAAAATTGGGATCGAGCTGGAGTGGGACGAAGCACAAGACTCTTACTTTTCCAGCTGCAGAGCATGCAAGTCCAACAATGGCTTCTGCGGCTTTAACTCCTCCGACCCAATTAAGCAATTCCTCTGTTTTTACGACGAAACTCACCTCACGCCGCCGTGGATTAGCAAAGACAACCCGAATCGAATCACCATCTTGTCGTCCGTGTTCACATTGGCTTGCTTCCTACTCGTCGTCTCTGTTGTCATAGCCGTCTTCAGGTCCAAAAGATTAAGCTCCGCCGCAACAGAAGAAGACCCAACAGCTCTGTTCCTCCACCGCCACCGCTCCGCCAACCTCCTCCCGCCAGTTTTCACATACGAAGAGCTTGAATCCTCAACAAACCAATTCGACCCGAAACGCAAAATCGGGGACGGTGGGTTCGGGTCTGTATATCTGGGTCAGCTCTACGACGGCAGAGTCGTCGCCGTAAAACACCTCCACAAACCCCTCCGCGGCGCCGCCGCATCCGGTAAAGCCTTCTCCAACAAATGCTTCTGCAACGAAATCCTAATCCTCTCCTCCATTGACCACCCAAATTTAGTCAAACTCCACGGCTACTGCAGCGACCCGAGAGGCCTCCTTCTCGTCTATGACTACGTCCCCAACGGCACCCTCGCGGACCACCTCCACGGCCCCAAGAGCCTCTACCGGAAAGGGTCGATGACGTGGCAGGTGAGAGTCGACATTGCTCTGCAAACCGCAATGGTTATGGAGTACCTGCACTTCTCGGTGGTGCCACCGGTGGTCCATAGAGACATAACGTCGTCGAATATCTTCGTCGAGAAAGATATGCGGATTAAAGTCGGGGACTTTGGGCTTTCCAGGCTCTTGGTTTTCCCCGAAAGGACGTCGTCCAGTTCCGGGTACGTTTGGACCGGCCCGCAGGGTACGCCTGGGTACTTGGACCCGGATTACCACCGGTCGTTCCGGTTGACCGACAAGAGTGACGTGTACAGTTTCGGTGTCGTTTTACTGGAGCTGATTTCGGGGCTGAAGGCGGTGGACCAGCGGAGGGACAAGAGGAAGCTGGCGCTGGCGGATTTGGTGGTGCAGAAAATCCAGACGGGCCTGCTACACCAGGTGGTGGACCCGGTTCTGGTTGTCGACGGGGACGTGTCCGACGGCGTGTATGCGGCGGCTGAGCTGGCGTTCAGGTGCGTTGCAGCGGATAAGGACGACAGGCCGGACGCCAAGGAAGTGGTGGAGGAGCTGAAGCGGATCAGGAGCCGCACACGTGGGGTGTCCCGGGCTTCGAGTTCGAACGTGATGGGTGAGGACGTGGCAAAGGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

659

Amino Acids

72.28

Weight (kDa)

7.78

Isoelectric Point (pI)

43.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WAK_assoc	PF14380	208 - 261	7e-06	Wall-associated receptor kinase C-terminal
Pkinase	PF00069	346 - 630	1.1e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	347 - 631	7.2e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69910
fragaria_vesca	FvH4_4g35620
malus_domestica	MD13G1081800.v1.1 MD16G1080900.v1.1
prunus_persica	Prupe.1G265100_v2.0.a1
pyrus_communis	pycom13g07060 pycom16g06990
rosa_chinensis	RchiOBHm_Chr4g0445241
rosa_laevigata	RLG00000005756
rosa_multiflora	Rmu_co8464497.1_g000001 Rmu_sc0000050.1_g000028 Rmu_sc0000050.1_g000029
rosa_roxburghii	Rroxscaffold_5G00385740
rosa_rugosa	Rorug04G0360000
rosa_samantha	Rh4AG421100 Rh4BG433000 Rh4CG447600 Rh4DG429000
rosa_wichuraiana	Rw4G036110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1345
AasI	GACNNNNNNGTC	1 cut(s)	1100
AatII	GACGTC	2 cut(s)	621, 1535
Acc36I	ACCTGC	2 cut(s)	1345, 1410
AccB1I	GGYRCC	3 cut(s)	1139, 1292, 1417
AccB7I	CCANNNNNTGG	1 cut(s)	1768
AccBSI	CCGCTC	2 cut(s)	414, 969
AccI	GTMKAC	2 cut(s)	1365, 1788
AccII	CGCG	3 cut(s)	89, 1137, 1301
AclWI	GGATC	5 cut(s)	97, 314, 583, 662, 1910
AcoI	YGGCCR	1 cut(s)	235
AcsI	RAATTY	1 cut(s)	1219
AcuI	CTGAAG	3 cut(s)	883, 1697, 1916
AcvI	CACGTG	1 cut(s)	1919
AcyI	GRCGYC	5 cut(s)	453, 618, 1140, 1532, 1872
AdeI	CACNNNGTG	1 cut(s)	1765
AfaI	GTAC	5 cut(s)	1400, 1550, 1573, 1583, 1637
AfiI	CCNNNNNNNGG	7 cut(s)	233, 900, 1136, 1545, 1696, 1768, 1780
AflIII	ACRYGT	3 cut(s)	1632, 1797, 1916
AgeI	ACCGGT	2 cut(s)	1423, 1602
AgsI	TTSAA	1 cut(s)	1013
AhdI	GACNNNNNGTC	1 cut(s)	1444
AjiI	CACGTC	4 cut(s)	1350, 1633, 1798, 1963
AjnI	CCWGG	4 cut(s)	226, 1505, 1576, 1761
AjuI	GAANNNNNNNTTGG	4 cut(s)	710, 742, 1160, 1192
AloI	GAACNNNNNNTCC	2 cut(s)	1046, 1078
Alw26I	GTCTC	2 cut(s)	883, 1431
AlwI	GGATC	5 cut(s)	97, 314, 583, 662, 1910
Ama87I	CYCGRG	2 cut(s)	1252, 1929
AoxI	GGCC	6 cut(s)	235, 1258, 1315, 1561, 1750, 1865
ApeKI	GCWGC	6 cut(s)	125, 513, 606, 699, 1245, 1847
ApoI	RAATTY	1 cut(s)	1219
ArsI	GACNNNNNNTTYG	6 cut(s)	146, 178, 368, 400, 1046, 1078
AsiGI	ACCGGT	2 cut(s)	1423, 1602
Asp700I	GAANNNNTTC	1 cut(s)	1457
AspLEI	GCGC	3 cut(s)	91, 1142, 1717
AsuC2I	CCSGG	5 cut(s)	1546, 1592, 1775, 1930, 1931
AsuHPI	GGTGA	7 cut(s)	189, 216, 586, 779, 823, 1369, 1967
AsuII	TTCGAA	2 cut(s)	442, 1943
AvaI	CYCGRG	2 cut(s)	1252, 1929
AvaII	GGWCC	7 cut(s)	903, 1303, 1429, 1557, 1588, 1687, 1771
BanI	GGYRCC	3 cut(s)	1139, 1292, 1417
BarI	GAAGNNNNNNTAC	2 cut(s)	1391, 1423
BbrPI	CACGTG	1 cut(s)	1919
BbsI	GAAGAC	2 cut(s)	889, 942
BbvI	GCAGC	6 cut(s)	112, 525, 593, 686, 1257, 1859
BccI	CCATC	7 cut(s)	166, 287, 293, 332, 425, 842, 1945
BceAI	ACGGC	9 cut(s)	190, 784, 878, 1094, 1111, 1252, 1306, 1330, 1822
BcgI	CGANNNNNNTGC	2 cut(s)	1087, 1121
BciT130I	CCWGG	4 cut(s)	228, 1507, 1578, 1763
BcnI	CCSGG	5 cut(s)	1546, 1592, 1775, 1930, 1931
BcoDI	GTCTC	2 cut(s)	883, 1431
BfaI	CTAG	1 cut(s)	408
BfmI	CTRYAG	3 cut(s)	511, 700, 1243
BfoI	RGCGCY	2 cut(s)	1143, 1718
BfuAI	ACCTGC	2 cut(s)	1345, 1410
BlpI	GCTNAGC	1 cut(s)	1824
Bme1390I	CCNGG	9 cut(s)	228, 1507, 1546, 1578, 1592, 1763, 1775, 1930, 1931
Bme18I	GGWCC	7 cut(s)	903, 1303, 1429, 1557, 1588, 1687, 1771
BmeRI	GACNNNNNGTC	1 cut(s)	1444
BmeT110I	CYCGRG	2 cut(s)	1252, 1929
BmgBI	CACGTC	4 cut(s)	1350, 1633, 1798, 1963
BmiI	GGNNCC	7 cut(s)	1141, 1294, 1318, 1419, 1590, 1773, 1910
BmrFI	CCNGG	9 cut(s)	228, 1507, 1546, 1578, 1592, 1763, 1775, 1930, 1931
BmsI	GCATC	2 cut(s)	221, 1157
BpiI	GAAGAC	2 cut(s)	889, 942
BpmI	CTGGAG	2 cut(s)	683, 1679
Bpu1102I	GCTNAGC	1 cut(s)	1824
Bpu14I	TTCGAA	2 cut(s)	442, 1943
BpuEI	CTTGAG	3 cut(s)	464, 539, 643
BpuMI	CCSGG	5 cut(s)	1546, 1592, 1775, 1930, 1931
BsaAI	YACGTR	1 cut(s)	1919
BsaHI	GRCGYC	5 cut(s)	453, 618, 1140, 1532, 1872
BsaJI	CCNNGG	8 cut(s)	524, 800, 1135, 1234, 1312, 1577, 1875, 1929
BsaWI	WCCGGW	5 cut(s)	1151, 1332, 1423, 1602, 1611
Bsc4I	CCNNNNNNNGG	7 cut(s)	233, 900, 1136, 1545, 1696, 1768, 1780
Bse118I	RCCGGY	3 cut(s)	1423, 1559, 1602
Bse1I	ACTGG	3 cut(s)	989, 1539, 1662
Bse3DI	GCAATG	3 cut(s)	517, 1368, 1392
BseBI	CCWGG	4 cut(s)	228, 1507, 1578, 1763
BseDI	CCNNGG	8 cut(s)	524, 800, 1135, 1234, 1312, 1577, 1875, 1929
BseGI	GGATG	3 cut(s)	50, 236, 1148
BseLI	CCNNNNNNNGG	7 cut(s)	233, 900, 1136, 1545, 1696, 1768, 1780
BseMI	GCAATG	3 cut(s)	517, 1368, 1392
BseMII	CTCAG	4 cut(s)	108, 135, 486, 1815
BseNI	ACTGG	3 cut(s)	989, 1539, 1662
BseRI	GAGGAG	6 cut(s)	322, 332, 733, 971, 1192, 1904
BseXI	GCAGC	6 cut(s)	112, 525, 593, 686, 1257, 1859
BseYI	CCCAGC	1 cut(s)	597
BsgI	GTGCAG	2 cut(s)	1388, 1754
Bsh1236I	CGCG	3 cut(s)	89, 1137, 1301
Bsh1285I	CGRYCG	1 cut(s)	1607
BshFI	GGCC	6 cut(s)	237, 1260, 1317, 1563, 1752, 1867
BshNI	GGYRCC	3 cut(s)	1139, 1292, 1417
BshTI	ACCGGT	2 cut(s)	1423, 1602
BsiEI	CGRYCG	1 cut(s)	1607
BsiHKCI	CYCGRG	2 cut(s)	1252, 1929
BslFI	GGGAC	8 cut(s)	542, 683, 1067, 1268, 1505, 1712, 1808, 1912
BslI	CCNNNNNNNGG	7 cut(s)	233, 900, 1136, 1545, 1696, 1768, 1780
BsmAI	GTCTC	2 cut(s)	883, 1431
BsmBI	CGTCTC	1 cut(s)	883
BsmFI	GGGAC	8 cut(s)	542, 683, 1067, 1268, 1505, 1712, 1808, 1912
BsnI	GGCC	6 cut(s)	237, 1260, 1317, 1563, 1752, 1867
BsoBI	CYCGRG	2 cut(s)	1252, 1929
Bsp119I	TTCGAA	2 cut(s)	442, 1943
Bsp1407I	TGTACA	1 cut(s)	1635
Bsp143I	GATC	5 cut(s)	102, 319, 575, 654, 1902
Bsp1720I	GCTNAGC	1 cut(s)	1824
BspANI	GGCC	6 cut(s)	237, 1260, 1317, 1563, 1752, 1867
BspCNI	CTCAG	4 cut(s)	107, 134, 485, 1816
BspFNI	CGCG	3 cut(s)	89, 1137, 1301
BspLI	GGNNCC	7 cut(s)	1141, 1294, 1318, 1419, 1590, 1773, 1910
BspMAI	CTGCAG	3 cut(s)	515, 704, 1247
BspMI	ACCTGC	2 cut(s)	1345, 1410
BspPI	GGATC	5 cut(s)	97, 314, 583, 662, 1910
BspQI	GCTCTTC	1 cut(s)	999
BspT104I	TTCGAA	2 cut(s)	442, 1943
BspT107I	GGYRCC	3 cut(s)	1139, 1292, 1417
BsrBI	CCGCTC	2 cut(s)	414, 969
BsrDI	GCAATG	3 cut(s)	517, 1368, 1392
BsrFI	RCCGGY	3 cut(s)	1423, 1559, 1602
BsrGI	TGTACA	1 cut(s)	1635
BsrI	ACTGG	3 cut(s)	989, 1539, 1662
BssAI	RCCGGY	3 cut(s)	1423, 1559, 1602
BssECI	CCNNGG	8 cut(s)	524, 800, 1135, 1234, 1312, 1577, 1875, 1929
BssMI	GATC	5 cut(s)	102, 319, 575, 654, 1902
BssNI	GRCGYC	5 cut(s)	453, 618, 1140, 1532, 1872
BssT1I	CCWWGG	2 cut(s)	524, 1875
Bst2UI	CCWGG	4 cut(s)	228, 1507, 1578, 1763
Bst4CI	ACNGT	3 cut(s)	370, 1058, 1640
Bst6I	CTCTTC	2 cut(s)	223, 999
BstACI	GRCGYC	5 cut(s)	453, 618, 1140, 1532, 1872
BstAPI	GCANNNNNTGC	2 cut(s)	519, 708
BstAUI	TGTACA	1 cut(s)	1635
BstBAI	YACGTR	1 cut(s)	1919
BstBI	TTCGAA	2 cut(s)	442, 1943
BstC8I	GCNNGC	8 cut(s)	536, 709, 862, 1565, 1713, 1719, 1754, 1830
BstDEI	CTNAG	4 cut(s)	94, 121, 472, 1824
BstDSI	CCRYGG	4 cut(s)	800, 1135, 1234, 1312
BstF5I	GGATG	3 cut(s)	50, 236, 1148
BstFNI	CGCG	3 cut(s)	89, 1137, 1301
BstH2I	RGCGCY	2 cut(s)	1143, 1718
BstHHI	GCGC	3 cut(s)	91, 1142, 1717
BstKTI	GATC	5 cut(s)	105, 322, 578, 657, 1905
BstMAI	GTCTC	2 cut(s)	883, 1431
BstMBI	GATC	5 cut(s)	102, 319, 575, 654, 1902
BstMCI	CGRYCG	1 cut(s)	1607
BstNI	CCWGG	4 cut(s)	228, 1507, 1578, 1763
BstNSI	RCATGY	1 cut(s)	711
BstSCI	CCNGG	9 cut(s)	226, 1505, 1544, 1576, 1590, 1761, 1773, 1928, 1929
BstSFI	CTRYAG	3 cut(s)	511, 700, 1243
BstUI	CGCG	3 cut(s)	89, 1137, 1301
BstV1I	GCAGC	6 cut(s)	112, 525, 593, 686, 1257, 1859
BstV2I	GAAGAC	2 cut(s)	889, 942
BstX2I	RGATCY	1 cut(s)	575
BstXI	CCANNNNNNTGG	2 cut(s)	1513, 1882
BstYI	RGATCY	1 cut(s)	575
BsuRI	GGCC	6 cut(s)	237, 1260, 1317, 1563, 1752, 1867
BtgI	CCRYGG	4 cut(s)	800, 1135, 1234, 1312
BtgZI	GCGATG	1 cut(s)	310
BtrI	CACGTC	4 cut(s)	1350, 1633, 1798, 1963
BtsCI	GGATG	3 cut(s)	50, 236, 1148
BveI	ACCTGC	2 cut(s)	1345, 1410
Cac8I	GCNNGC	8 cut(s)	536, 709, 862, 1565, 1713, 1719, 1754, 1830
CfoI	GCGC	3 cut(s)	91, 1142, 1717
Cfr10I	RCCGGY	3 cut(s)	1423, 1559, 1602
Cfr42I	CCGCGG	1 cut(s)	1138
Cfr9I	CCCGGG	1 cut(s)	1929
CseI	GACGC	2 cut(s)	461, 1880
CsiI	ACCWGGT	1 cut(s)	1761
Csp6I	GTAC	5 cut(s)	1399, 1549, 1572, 1582, 1636
CspAI	ACCGGT	2 cut(s)	1423, 1602
CviAII	CATG	4 cut(s)	55, 144, 180, 708
CviQI	GTAC	5 cut(s)	1399, 1549, 1572, 1582, 1636
DdeI	CTNAG	4 cut(s)	94, 121, 472, 1824
DinI	GGCGCC	1 cut(s)	1141
DpnI	GATC	5 cut(s)	104, 321, 577, 656, 1904
DpnII	GATC	5 cut(s)	102, 319, 575, 654, 1902
DraIII	CACNNNGTG	1 cut(s)	1765
DrdI	GACNNNNNNGTC	1 cut(s)	1100
DriI	GACNNNNNGTC	1 cut(s)	1444
DseDI	GACNNNNNNGTC	1 cut(s)	1100
EaeI	YGGCCR	1 cut(s)	235
Eam1104I	CTCTTC	2 cut(s)	223, 999
Eam1105I	GACNNNNNGTC	1 cut(s)	1444
EarI	CTCTTC	2 cut(s)	223, 999
EciI	GGCGGA	4 cut(s)	418, 910, 961, 1736
Eco130I	CCWWGG	2 cut(s)	524, 1875
Eco147I	AGGCCT	1 cut(s)	1260
Eco47I	GGWCC	7 cut(s)	903, 1303, 1429, 1557, 1588, 1687, 1771
Eco57I	CTGAAG	3 cut(s)	883, 1697, 1916
Eco72I	CACGTG	1 cut(s)	1919
Eco88I	CYCGRG	2 cut(s)	1252, 1929
EcoRII	CCWGG	4 cut(s)	226, 1505, 1576, 1761
EcoT14I	CCWWGG	2 cut(s)	524, 1875
EgeI	GGCGCC	1 cut(s)	1141
EheI	GGCGCC	1 cut(s)	1141
ErhI	CCWWGG	2 cut(s)	524, 1875
Esp3I	CGTCTC	1 cut(s)	883
FaeI	CATG	4 cut(s)	58, 147, 183, 711
FalI	AAGNNNNNCTT	2 cut(s)	473, 505
FaqI	GGGAC	8 cut(s)	542, 683, 1067, 1268, 1505, 1712, 1808, 1912
FatI	CATG	4 cut(s)	54, 143, 179, 707
FauI	CCCGC	3 cut(s)	473, 993, 1572
FblI	GTMKAC	2 cut(s)	1365, 1788
FokI	GGATG	3 cut(s)	37, 243, 1135
FspBI	CTAG	1 cut(s)	408
GlaI	GCGC	3 cut(s)	90, 1141, 1716
GsaI	CCCAGC	1 cut(s)	601
GsuI	CTGGAG	2 cut(s)	683, 1679
HaeII	RGCGCY	2 cut(s)	1143, 1718
HaeIII	GGCC	6 cut(s)	237, 1260, 1317, 1563, 1752, 1867
HgaI	GACGC	2 cut(s)	461, 1880
HhaI	GCGC	3 cut(s)	91, 1142, 1717
Hin1I	GRCGYC	5 cut(s)	453, 618, 1140, 1532, 1872
Hin1II	CATG	4 cut(s)	58, 147, 183, 711
Hin6I	GCGC	3 cut(s)	89, 1140, 1715
HinP1I	GCGC	3 cut(s)	89, 1140, 1715
HincII	GTYRAC	3 cut(s)	1366, 1617, 1789
HindII	GTYRAC	3 cut(s)	1366, 1617, 1789
HindIII	AAGCTT	1 cut(s)	557
HinfI	GANTC	6 cut(s)	683, 823, 828, 1013, 1101, 1362
HphI	GGTGA	7 cut(s)	189, 216, 586, 779, 823, 1369, 1967
Hpy166II	GTNNAC	8 cut(s)	185, 852, 1366, 1617, 1636, 1687, 1771, 1789
Hpy188I	TCNGA	3 cut(s)	451, 748, 1804
Hpy188III	TCNNGA	6 cut(s)	100, 622, 632, 1465, 1744, 1906
Hpy8I	GTNNAC	8 cut(s)	185, 852, 1366, 1617, 1636, 1687, 1771, 1789
HpyAV	CCTTC	9 cut(s)	37, 54, 175, 223, 252, 408, 1171, 1274, 1672
HpyCH4III	ACNGT	3 cut(s)	370, 1058, 1640
HpyF3I	CTNAG	4 cut(s)	94, 121, 472, 1824
Hsp92I	GRCGYC	5 cut(s)	453, 618, 1140, 1532, 1872
Hsp92II	CATG	4 cut(s)	58, 147, 183, 711
HspAI	GCGC	3 cut(s)	89, 1140, 1715
KasI	GGCGCC	1 cut(s)	1139
KspI	CCGCGG	1 cut(s)	1138
Kzo9I	GATC	5 cut(s)	102, 319, 575, 654, 1902
LguI	GCTCTTC	1 cut(s)	999
LmnI	GCTCC	7 cut(s)	419, 467, 923, 974, 1660, 1891, 1908
Lsp1109I	GCAGC	6 cut(s)	112, 525, 593, 686, 1257, 1859
LweI	GCATC	2 cut(s)	221, 1157
MabI	ACCWGGT	1 cut(s)	1761
MaeI	CTAG	1 cut(s)	408
MaeIII	GTNAC	3 cut(s)	370, 592, 1628
MalI	GATC	5 cut(s)	104, 321, 577, 656, 1904
MbiI	CCGCTC	2 cut(s)	414, 969
MboI	GATC	5 cut(s)	102, 319, 575, 654, 1902
MboII	GAAGA	8 cut(s)	210, 213, 494, 889, 944, 947, 1016, 1450
MflI	RGATCY	1 cut(s)	575
MluCI	AATT	5 cut(s)	648, 753, 761, 1028, 1219
Mly113I	GGCGCC	1 cut(s)	1140
MlyI	GAGTC	3 cut(s)	677, 1110, 1371
MmeI	TCCRAC	5 cut(s)	474, 741, 771, 1191, 1827
MroXI	GAANNNNTTC	1 cut(s)	1457
MseI	TTAA	4 cut(s)	738, 756, 914, 1482
MslI	CAYNNNNRTG	3 cut(s)	53, 178, 229
MspA1I	CMGCKG	5 cut(s)	125, 699, 1137, 1693, 1850
MspR9I	CCNGG	9 cut(s)	228, 1507, 1546, 1578, 1592, 1763, 1775, 1930, 1931
MvaI	CCWGG	4 cut(s)	228, 1507, 1578, 1763
MvnI	CGCG	3 cut(s)	89, 1137, 1301
NarI	GGCGCC	1 cut(s)	1140
NciI	CCSGG	5 cut(s)	1546, 1592, 1775, 1930, 1931
NdeII	GATC	5 cut(s)	102, 319, 575, 654, 1902
NlaIII	CATG	4 cut(s)	58, 147, 183, 711
NlaIV	GGNNCC	7 cut(s)	1141, 1294, 1318, 1419, 1590, 1773, 1910
NmuCI	GTSAC	3 cut(s)	370, 592, 1628
NspI	RCATGY	1 cut(s)	711
NspV	TTCGAA	2 cut(s)	442, 1943
PaeI	GCATGC	1 cut(s)	711
PaqCI	CACCTGC	1 cut(s)	1345
PceI	AGGCCT	1 cut(s)	1260
PciSI	GCTCTTC	1 cut(s)	999
PcsI	WCGNNNNNNNCGW	2 cut(s)	1101, 1794
PdmI	GAANNNNTTC	1 cut(s)	1457
PfeI	GAWTC	3 cut(s)	823, 828, 1013
PflFI	GACNNNGTC	3 cut(s)	617, 1280, 1534
PflMI	CCANNNNNTGG	1 cut(s)	1768
PinAI	ACCGGT	2 cut(s)	1423, 1602
PleI	GAGTC	3 cut(s)	677, 1109, 1370
PluTI	GGCGCC	1 cut(s)	1143
PmaCI	CACGTG	1 cut(s)	1919
PmlI	CACGTG	1 cut(s)	1919
PpsI	GAGTC	3 cut(s)	677, 1109, 1370
Ppu21I	YACGTR	1 cut(s)	1919
Psp6I	CCWGG	4 cut(s)	226, 1505, 1576, 1761
PspCI	CACGTG	1 cut(s)	1919
PspFI	CCCAGC	1 cut(s)	597
PspGI	CCWGG	4 cut(s)	226, 1505, 1576, 1761
PspN4I	GGNNCC	7 cut(s)	1141, 1294, 1318, 1419, 1590, 1773, 1910
PstI	CTGCAG	3 cut(s)	515, 704, 1247
PsuI	RGATCY	1 cut(s)	575
PsyI	GACNNNGTC	3 cut(s)	617, 1280, 1534
PvuII	CAGCTG	2 cut(s)	125, 699
RsaI	GTAC	5 cut(s)	1400, 1550, 1573, 1583, 1637
RsaNI	GTAC	5 cut(s)	1399, 1549, 1572, 1582, 1636
RseI	CAYNNNNRTG	3 cut(s)	53, 178, 229
SacII	CCGCGG	1 cut(s)	1138
SalI	GTCGAC	2 cut(s)	1364, 1787
SapI	GCTCTTC	1 cut(s)	999
SaqAI	TTAA	4 cut(s)	738, 756, 914, 1482
Sau3AI	GATC	5 cut(s)	102, 319, 575, 654, 1902
SchI	GAGTC	3 cut(s)	677, 1110, 1371
ScrFI	CCNGG	9 cut(s)	228, 1507, 1546, 1578, 1592, 1763, 1775, 1930, 1931
SexAI	ACCWGGT	1 cut(s)	1761
SfaNI	GCATC	2 cut(s)	221, 1157
SfcI	CTRYAG	3 cut(s)	511, 700, 1243
SfoI	GGCGCC	1 cut(s)	1141
Sfr303I	CCGCGG	1 cut(s)	1138
SfuI	TTCGAA	2 cut(s)	442, 1943
SgrAI	CRCCGGYG	1 cut(s)	1423
SgrBI	CCGCGG	1 cut(s)	1138
SinI	GGWCC	7 cut(s)	903, 1303, 1429, 1557, 1588, 1687, 1771
SmaI	CCCGGG	1 cut(s)	1931
SmiMI	CAYNNNNRTG	3 cut(s)	53, 178, 229
SmlI	CTYRAG	3 cut(s)	479, 554, 622
SmoI	CTYRAG	3 cut(s)	479, 554, 622
SphI	GCATGC	1 cut(s)	711
Sse9I	AATT	5 cut(s)	648, 753, 761, 1028, 1219
SseBI	AGGCCT	1 cut(s)	1260
SspDI	GGCGCC	1 cut(s)	1139
SspMI	CTAG	1 cut(s)	408
StuI	AGGCCT	1 cut(s)	1260
StyD4I	CCNGG	9 cut(s)	226, 1505, 1544, 1576, 1590, 1761, 1773, 1928, 1929
StyI	CCWWGG	2 cut(s)	524, 1875
TaaI	ACNGT	3 cut(s)	370, 1058, 1640
TaqII	GACCGA	1 cut(s)	1634
TasI	AATT	5 cut(s)	648, 753, 761, 1028, 1219
TatI	WGTACW	1 cut(s)	1635
TfiI	GAWTC	3 cut(s)	823, 828, 1013
Tru1I	TTAA	4 cut(s)	738, 756, 914, 1482
Tru9I	TTAA	4 cut(s)	738, 756, 914, 1482
TseFI	GTSAC	3 cut(s)	370, 592, 1628
TseI	GCWGC	6 cut(s)	125, 513, 606, 699, 1245, 1847
Tsp45I	GTSAC	3 cut(s)	370, 592, 1628
TspDTI	ATGAA	1 cut(s)	168
TspGWI	ACGGA	2 cut(s)	73, 835
TspMI	CCCGGG	1 cut(s)	1929
Tth111I	GACNNNGTC	3 cut(s)	617, 1280, 1534
Van91I	CCANNNNNTGG	1 cut(s)	1768
VpaK11BI	GGWCC	7 cut(s)	903, 1303, 1429, 1557, 1588, 1687, 1771
XapI	RAATTY	1 cut(s)	1219
XceI	RCATGY	1 cut(s)	711
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1882
XmaI	CCCGGG	1 cut(s)	1929
XmiI	GTMKAC	2 cut(s)	1365, 1788
XmnI	GAANNNNTTC	1 cut(s)	1457
XspI	CTAG	1 cut(s)	408
ZraI	GACGTC	2 cut(s)	619, 1533

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.