Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0012229

Physical Location & Seq

Genomic Coordinates Forward (+)

4683888 .. 4685798

Gene Structure 1911 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom13g07060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGCCTCCACCACACTCCGTCGCACTCTCAGCCCTGATCCTCCTAACACTCTACTCAGCTACCACCGCCGCCCACTCATGTTTCCCAAAACCATCGTCCTCCTCCACATTTTCCTGTCCACCATTCACTTCGCCGCCGCCGTTCCCCTTCTCTTCTTCACCAGGCTGCGGCCACCCTTCTTTCCAAATCCAATGCTCTGCCCCCCACTCCACCATCTCCATCAATAACCTCACTTTCTCTCTCATCAGCTACGATGCCAACGCCTCTTCACTCCTTCTCTCTCCCCACACCACCAACTTAACTGCCACAAATTGCTCATCCTCTCACTATTCTTCCATCCCCAGCCGATCCATCAACATCTCCGCCTCCCCATTTCGAATATCCGACGGCTCCTGCTCCCGCCTCTCAGTTCTCAAGCCTTGTTCCCCGCCGAATCTTCCCAACTGCAGCCACTGCCCTTGGGAATGCAAGCTCATCAAAGACCCACTCAAGCTTCTCCATGACTGCGGATCTCCGCACCCCCCTGTCACCCACCAGGGCTGCCACGCCGACGTTTTGAGCTTTCTCGACGATTTTCTCAAAATTGGGATCGAGCTGGAGTGGGACGAAGCTCAGGACTCTTACTTTTCCAGATGCAGAGCTTGCAAGTCCAACAATGGCTTCTGCGGCTTTAACTCCTCCGACCCAAATAAGCAATTCCTCTGTTTTCACGCCGAACCTCACCTTACGCCGCCATTGATCAGCAAAGACAACCCGAATCGAATCGCCATCTTGTCCTCTGTGTTCACATTAACTTGCTTCCTACTCGTCGCCTCTGTTATCATAGCCGTCTTCAGGTCCAAAAGATTAAGCTCGGCCGCAACAGAGGAAGACCCAACAACTCTGTTCCTCCACCGCCACCGCCCCGCCAACCTCCTCCCGCCAGTTTTCACATACGAAGAGCTCGAATCCTCAACAAACCACTTCGACCCGAAACGCAAAATCGGGGACGGCGGGTTCGGGTCTGTCTATCTGGGTCAGCTCTACGATGGCAGAGTTGTTGCCGTAAAATACCTCCACAAACCCCTCCGCGGCGCCGCCGCATCCGGGAAAGCCTTCTCCAACAAATGCTTCTGCAACGAAATCCTAATCCTCTCCTCCATTGACCACCCAAATTTAGTCAAACTCCACGGCTACTGCAGCGACCCGAGAGGCCTCCTTCTAGTCTACGACTACGTTCCCAACGGCACCCTCGCCGACCATCTCCACGGCCCCAAGAGCCTCCATCGGAAAGGGTCGATGACGTGGCAGGTGAGGGTCGACATTGCTCTGCAAACGGCCATGGTTATGGAGTACCTGCACTTCTCGGTGGTGCCGCCAGTGGTCCATAGAGACATAACGTCGTCGAATATCTTCATCGAGAAAGATATGCGGGTTAAAGTCGGGGACTTTGGGCTTTCCAGGCTGTTGGTATTCCCCGAAACGACGTCGTCCAGCTCCGGGTACGTTTGGACCGGTCCGCAGGGGACGCCCGGTTACTTGGACCCGGATTACCACCGGTCGTTCAGGCTGACCGACAAGAGTGACGTGTACAGTTTCGGTGTCGTTTTACTGGAGCTGATTTCGGGGCTGAAGGCGGTGGACCAGCGGAGGGACAAGCGGGAGCTGGCGCTGGCGGATTTGGTGGTGTCGAAAATCCAGATGGGCCTGCTCCACCAGGTAGTAGACCCGGTTCTGGTCGTCGACGGTGACGTATCCGACGGCGTGGATGCGGCGGCTGAGCTGGCGTTCAGGTGCGTTGCGGCGGATAAGGACGACAGGCCGGACGCCAAGGAAGTGGTGGAGGAGCTGAAGCGGATCAGAAGCCGCACTCGTGGGGTGTCCCGGGTTTCGAGTTCGAATGTGATTGGCGACGACGTGACAAAGGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

637

Amino Acids

69.55

Weight (kDa)

7.11

Isoelectric Point (pI)

43.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	324 - 608	6.8e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	325 - 543	9.5e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69910
fragaria_vesca	FvH4_4g35620
malus_domestica	MD13G1081800.v1.1 MD16G1080900.v1.1
prunus_persica	Prupe.1G265100_v2.0.a1
pyrus_communis	pycom13g07060 pycom16g06990
rosa_chinensis	RchiOBHm_Chr4g0445241
rosa_laevigata	RLG00000005756
rosa_multiflora	Rmu_co8464497.1_g000001 Rmu_sc0000050.1_g000028 Rmu_sc0000050.1_g000029
rosa_roxburghii	Rroxscaffold_5G00385740
rosa_rugosa	Rorug04G0360000
rosa_samantha	Rh4AG421100 Rh4BG433000 Rh4CG447600 Rh4DG429000
rosa_wichuraiana	Rw4G036110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1279
AatII	GACGTC	1 cut(s)	1469
Acc36I	ACCTGC	2 cut(s)	1279, 1344
AccB1I	GGYRCC	3 cut(s)	1073, 1226, 1351
AccI	GTMKAC	4 cut(s)	1206, 1299, 1704, 1722
AccII	CGCG	1 cut(s)	1071
AclWI	GGATC	5 cut(s)	31, 342, 517, 596, 1844
AcoI	YGGCCR	3 cut(s)	169, 855, 1317
AcsI	RAATTY	1 cut(s)	1153
AcuI	CTGAAG	3 cut(s)	817, 1631, 1850
AcyI	GRCGYC	4 cut(s)	1074, 1466, 1508, 1806
AfaI	GTAC	3 cut(s)	1334, 1484, 1571
AfiI	CCNNNNNNNGG	7 cut(s)	167, 834, 1070, 1086, 1479, 1630, 1714
AflIII	ACRYGT	1 cut(s)	1566
AgeI	ACCGGT	2 cut(s)	1493, 1536
AhdI	GACNNNNNGTC	1 cut(s)	1378
AjiI	CACGTC	3 cut(s)	1284, 1567, 1897
AjnI	CCWGG	4 cut(s)	160, 534, 1439, 1695
AjuI	GAANNNNNNNTTGG	4 cut(s)	644, 676, 1094, 1126
AloI	GAACNNNNNNTCC	2 cut(s)	980, 1012
Alw21I	GWGCWC	1 cut(s)	945
Alw26I	GTCTC	1 cut(s)	1365
AlwI	GGATC	5 cut(s)	31, 342, 517, 596, 1844
AlwNI	CAGNNNCTG	1 cut(s)	453
Ama87I	CYCGRG	2 cut(s)	1186, 1863
AoxI	GGCC	7 cut(s)	169, 855, 1192, 1249, 1317, 1684, 1799
ApeKI	GCWGC	4 cut(s)	165, 447, 540, 1179
ApoI	RAATTY	1 cut(s)	1153
ArsI	GACNNNNNNTTYG	4 cut(s)	80, 112, 980, 1012
AsiGI	ACCGGT	2 cut(s)	1493, 1536
Asp700I	GAANNNNTTC	2 cut(s)	1094, 1391
AspLEI	GCGC	2 cut(s)	1076, 1651
AspS9I	GGNCC	8 cut(s)	837, 1250, 1363, 1491, 1496, 1522, 1621, 1684
AsuC2I	CCSGG	7 cut(s)	1087, 1480, 1512, 1526, 1709, 1864, 1865
AsuHPI	GGTGA	5 cut(s)	150, 520, 713, 1303, 1739
AsuII	TTCGAA	2 cut(s)	376, 1877
AvaI	CYCGRG	2 cut(s)	1186, 1863
AvaII	GGWCC	6 cut(s)	837, 1363, 1491, 1496, 1522, 1621
BanI	GGYRCC	3 cut(s)	1073, 1226, 1351
BanII	GRGCYC	1 cut(s)	945
BarI	GAAGNNNNNNTAC	2 cut(s)	1325, 1357
BauI	CACGAG	1 cut(s)	1851
BbsI	GAAGAC	2 cut(s)	823, 876
Bbv12I	GWGCWC	1 cut(s)	945
BbvI	GCAGC	4 cut(s)	152, 459, 527, 1191
BciT130I	CCWGG	4 cut(s)	162, 536, 1441, 1697
BciVI	GTATCC	1 cut(s)	1744
BclI	TGATCA	1 cut(s)	738
BcnI	CCSGG	7 cut(s)	1087, 1480, 1512, 1526, 1709, 1864, 1865
BcoDI	GTCTC	1 cut(s)	1365
BfaI	CTAG	1 cut(s)	1202
BfmI	CTRYAG	2 cut(s)	445, 1177
BfoI	RGCGCY	2 cut(s)	1077, 1652
BfuAI	ACCTGC	2 cut(s)	1279, 1344
BfuI	GTATCC	1 cut(s)	1744
BlpI	GCTNAGC	1 cut(s)	1758
Bme18I	GGWCC	6 cut(s)	837, 1363, 1491, 1496, 1522, 1621
BmeRI	GACNNNNNGTC	1 cut(s)	1378
BmeT110I	CYCGRG	2 cut(s)	1186, 1863
BmgBI	CACGTC	3 cut(s)	1284, 1567, 1897
BmgT120I	GGNCC	8 cut(s)	837, 1250, 1363, 1491, 1496, 1522, 1621, 1684
BmiI	GGNNCC	6 cut(s)	391, 1075, 1228, 1252, 1353, 1524
BmsI	GCATC	4 cut(s)	244, 623, 1091, 1738
BpiI	GAAGAC	2 cut(s)	823, 876
BpmI	CTGGAG	2 cut(s)	617, 1613
Bpu10I	CCTNAGC	1 cut(s)	612
Bpu1102I	GCTNAGC	1 cut(s)	1758
Bpu14I	TTCGAA	2 cut(s)	376, 1877
BpuEI	CTTGAG	2 cut(s)	398, 473
BpuMI	CCSGG	7 cut(s)	1087, 1480, 1512, 1526, 1709, 1864, 1865
BsaHI	GRCGYC	4 cut(s)	1074, 1466, 1508, 1806
BsaJI	CCNNGG	8 cut(s)	458, 535, 1069, 1168, 1246, 1320, 1809, 1863
BsaWI	WCCGGW	2 cut(s)	1493, 1536
Bsc4I	CCNNNNNNNGG	7 cut(s)	167, 834, 1070, 1086, 1479, 1630, 1714
Bse118I	RCCGGY	2 cut(s)	1493, 1536
Bse1I	ACTGG	3 cut(s)	923, 1358, 1596
Bse3DI	GCAATG	1 cut(s)	1302
BseBI	CCWGG	4 cut(s)	162, 536, 1441, 1697
BseDI	CCNNGG	8 cut(s)	458, 535, 1069, 1168, 1246, 1320, 1809, 1863
BseGI	GGATG	4 cut(s)	317, 336, 1082, 1753
BseLI	CCNNNNNNNGG	7 cut(s)	167, 834, 1070, 1086, 1479, 1630, 1714
BseMI	GCAATG	1 cut(s)	1302
BseMII	CTCAG	5 cut(s)	42, 69, 420, 626, 1749
BseNI	ACTGG	3 cut(s)	923, 1358, 1596
BseRI	GAGGAG	5 cut(s)	91, 667, 905, 1126, 1838
BseX3I	CGGCCG	1 cut(s)	855
BseXI	GCAGC	4 cut(s)	152, 459, 527, 1191
BseYI	CCCAGC	1 cut(s)	341
BsgI	GTGCAG	1 cut(s)	1322
Bsh1236I	CGCG	1 cut(s)	1071
Bsh1285I	CGRYCG	2 cut(s)	858, 1541
BshFI	GGCC	7 cut(s)	171, 857, 1194, 1251, 1319, 1686, 1801
BshNI	GGYRCC	3 cut(s)	1073, 1226, 1351
BshTI	ACCGGT	2 cut(s)	1493, 1536
BsiEI	CGRYCG	2 cut(s)	858, 1541
BsiHKAI	GWGCWC	1 cut(s)	945
BsiHKCI	CYCGRG	2 cut(s)	1186, 1863
BsiSI	CCGG	9 cut(s)	1086, 1479, 1494, 1512, 1526, 1537, 1709, 1802, 1864
BslFI	GGGAC	6 cut(s)	617, 1001, 1439, 1519, 1646, 1846
BslI	CCNNNNNNNGG	7 cut(s)	167, 834, 1070, 1086, 1479, 1630, 1714
BsmAI	GTCTC	1 cut(s)	1365
BsmFI	GGGAC	6 cut(s)	617, 1001, 1439, 1519, 1646, 1846
BsmI	GAATGC	1 cut(s)	470
BsnI	GGCC	7 cut(s)	171, 857, 1194, 1251, 1319, 1686, 1801
BsoBI	CYCGRG	2 cut(s)	1186, 1863
Bsp119I	TTCGAA	2 cut(s)	376, 1877
Bsp1286I	GDGCHC	1 cut(s)	945
Bsp1407I	TGTACA	1 cut(s)	1569
Bsp143I	GATC	6 cut(s)	36, 347, 509, 588, 738, 1836
Bsp1720I	GCTNAGC	1 cut(s)	1758
Bsp19I	CCATGG	1 cut(s)	1320
BspANI	GGCC	7 cut(s)	171, 857, 1194, 1251, 1319, 1686, 1801
BspCNI	CTCAG	5 cut(s)	41, 68, 419, 625, 1750
BspFNI	CGCG	1 cut(s)	1071
BspLI	GGNNCC	6 cut(s)	391, 1075, 1228, 1252, 1353, 1524
BspMAI	CTGCAG	2 cut(s)	449, 1181
BspMI	ACCTGC	2 cut(s)	1279, 1344
BspPI	GGATC	5 cut(s)	31, 342, 517, 596, 1844
BspQI	GCTCTTC	1 cut(s)	933
BspT104I	TTCGAA	2 cut(s)	376, 1877
BspT107I	GGYRCC	3 cut(s)	1073, 1226, 1351
BsrDI	GCAATG	1 cut(s)	1302
BsrFI	RCCGGY	2 cut(s)	1493, 1536
BsrGI	TGTACA	1 cut(s)	1569
BsrI	ACTGG	3 cut(s)	923, 1358, 1596
BssAI	RCCGGY	2 cut(s)	1493, 1536
BssECI	CCNNGG	8 cut(s)	458, 535, 1069, 1168, 1246, 1320, 1809, 1863
BssMI	GATC	6 cut(s)	36, 347, 509, 588, 738, 1836
BssNI	GRCGYC	4 cut(s)	1074, 1466, 1508, 1806
BssSI	CACGAG	1 cut(s)	1851
BssT1I	CCWWGG	3 cut(s)	458, 1320, 1809
Bst2BI	CACGAG	1 cut(s)	1851
Bst2UI	CCWGG	4 cut(s)	162, 536, 1441, 1697
Bst4CI	ACNGT	2 cut(s)	1574, 1727
Bst6I	CTCTTC	3 cut(s)	157, 271, 933
BstACI	GRCGYC	4 cut(s)	1074, 1466, 1508, 1806
BstAPI	GCANNNNNTGC	2 cut(s)	453, 642
BstAUI	TGTACA	1 cut(s)	1569
BstBI	TTCGAA	2 cut(s)	376, 1877
BstC8I	GCNNGC	6 cut(s)	470, 643, 1647, 1653, 1688, 1764
BstDEI	CTNAG	5 cut(s)	28, 55, 406, 612, 1758
BstDSI	CCRYGG	4 cut(s)	1069, 1168, 1246, 1320
BstF5I	GGATG	4 cut(s)	317, 336, 1082, 1753
BstFNI	CGCG	1 cut(s)	1071
BstH2I	RGCGCY	2 cut(s)	1077, 1652
BstHHI	GCGC	2 cut(s)	1076, 1651
BstKTI	GATC	6 cut(s)	39, 350, 512, 591, 741, 1839
BstMAI	GTCTC	1 cut(s)	1365
BstMBI	GATC	6 cut(s)	36, 347, 509, 588, 738, 1836
BstMCI	CGRYCG	2 cut(s)	858, 1541
BstNI	CCWGG	4 cut(s)	162, 536, 1441, 1697
BstSFI	CTRYAG	2 cut(s)	445, 1177
BstUI	CGCG	1 cut(s)	1071
BstV1I	GCAGC	4 cut(s)	152, 459, 527, 1191
BstV2I	GAAGAC	2 cut(s)	823, 876
BstX2I	RGATCY	1 cut(s)	509
BstXI	CCANNNNNNTGG	3 cut(s)	1327, 1447, 1816
BstYI	RGATCY	1 cut(s)	509
BstZI	CGGCCG	1 cut(s)	855
BsuI	GTATCC	1 cut(s)	1744
BsuRI	GGCC	7 cut(s)	171, 857, 1194, 1251, 1319, 1686, 1801
BtgI	CCRYGG	4 cut(s)	1069, 1168, 1246, 1320
BtrI	CACGTC	3 cut(s)	1284, 1567, 1897
BtsCI	GGATG	4 cut(s)	317, 336, 1082, 1753
BtsI	GCAGTG	1 cut(s)	451
BtsIMutI	CAGTG	2 cut(s)	451, 1365
BveI	ACCTGC	2 cut(s)	1279, 1344
Cac8I	GCNNGC	6 cut(s)	470, 643, 1647, 1653, 1688, 1764
CaiI	CAGNNNCTG	1 cut(s)	453
CfoI	GCGC	2 cut(s)	1076, 1651
Cfr10I	RCCGGY	2 cut(s)	1493, 1536
Cfr13I	GGNCC	8 cut(s)	837, 1250, 1363, 1491, 1496, 1522, 1621, 1684
Cfr42I	CCGCGG	1 cut(s)	1072
Cfr9I	CCCGGG	1 cut(s)	1863
CpoI	CGGWCCG	1 cut(s)	1496
CseI	GACGC	2 cut(s)	1516, 1814
CsiI	ACCWGGT	1 cut(s)	1695
Csp6I	GTAC	3 cut(s)	1333, 1483, 1570
CspAI	ACCGGT	2 cut(s)	1493, 1536
CspCI	CAANNNNNGTGG	2 cut(s)	440, 475
CspI	CGGWCCG	1 cut(s)	1496
CviAII	CATG	3 cut(s)	78, 500, 1321
CviQI	GTAC	3 cut(s)	1333, 1483, 1570
DdeI	CTNAG	5 cut(s)	28, 55, 406, 612, 1758
DinI	GGCGCC	1 cut(s)	1075
DpnI	GATC	6 cut(s)	38, 349, 511, 590, 740, 1838
DpnII	GATC	6 cut(s)	36, 347, 509, 588, 738, 1836
DriI	GACNNNNNGTC	1 cut(s)	1378
EaeI	YGGCCR	3 cut(s)	169, 855, 1317
EagI	CGGCCG	1 cut(s)	855
Eam1104I	CTCTTC	3 cut(s)	157, 271, 933
Eam1105I	GACNNNNNGTC	1 cut(s)	1378
EarI	CTCTTC	3 cut(s)	157, 271, 933
EciI	GGCGGA	3 cut(s)	352, 1670, 1799
Ecl136II	GAGCTC	1 cut(s)	943
EclXI	CGGCCG	1 cut(s)	855
Eco130I	CCWWGG	3 cut(s)	458, 1320, 1809
Eco147I	AGGCCT	1 cut(s)	1194
Eco24I	GRGCYC	1 cut(s)	945
Eco47I	GGWCC	6 cut(s)	837, 1363, 1491, 1496, 1522, 1621
Eco52I	CGGCCG	1 cut(s)	855
Eco53kI	GAGCTC	1 cut(s)	943
Eco57I	CTGAAG	3 cut(s)	817, 1631, 1850
Eco88I	CYCGRG	2 cut(s)	1186, 1863
EcoICRI	GAGCTC	1 cut(s)	943
EcoRII	CCWGG	4 cut(s)	160, 534, 1439, 1695
EcoT14I	CCWWGG	3 cut(s)	458, 1320, 1809
EcoT38I	GRGCYC	1 cut(s)	945
EgeI	GGCGCC	1 cut(s)	1075
EheI	GGCGCC	1 cut(s)	1075
ErhI	CCWWGG	3 cut(s)	458, 1320, 1809
FaeI	CATG	3 cut(s)	81, 503, 1324
FaiI	YATR	9 cut(s)	79, 501, 824, 934, 1322, 1328, 1368, 1376, 1409
FaqI	GGGAC	6 cut(s)	617, 1001, 1439, 1519, 1646, 1846
FatI	CATG	3 cut(s)	77, 499, 1320
FauI	CCCGC	7 cut(s)	407, 435, 913, 927, 986, 1404, 1632
FbaI	TGATCA	1 cut(s)	738
FblI	GTMKAC	4 cut(s)	1206, 1299, 1704, 1722
FokI	GGATG	4 cut(s)	304, 323, 1069, 1760
FriOI	GRGCYC	1 cut(s)	945
FspBI	CTAG	1 cut(s)	1202
GlaI	GCGC	2 cut(s)	1075, 1650
GsaI	CCCAGC	1 cut(s)	345
GsuI	CTGGAG	2 cut(s)	617, 1613
HaeII	RGCGCY	2 cut(s)	1077, 1652
HaeIII	GGCC	7 cut(s)	171, 857, 1194, 1251, 1319, 1686, 1801
HapII	CCGG	9 cut(s)	1086, 1479, 1494, 1512, 1526, 1537, 1709, 1802, 1864
HgaI	GACGC	2 cut(s)	1516, 1814
HhaI	GCGC	2 cut(s)	1076, 1651
Hin1I	GRCGYC	4 cut(s)	1074, 1466, 1508, 1806
Hin1II	CATG	3 cut(s)	81, 503, 1324
Hin6I	GCGC	2 cut(s)	1074, 1649
HinP1I	GCGC	2 cut(s)	1074, 1649
HincII	GTYRAC	2 cut(s)	1300, 1723
HindII	GTYRAC	2 cut(s)	1300, 1723
HindIII	AAGCTT	1 cut(s)	491
HinfI	GANTC	5 cut(s)	433, 617, 757, 762, 947
HpaII	CCGG	9 cut(s)	1086, 1479, 1494, 1512, 1526, 1537, 1709, 1802, 1864
HphI	GGTGA	5 cut(s)	150, 520, 713, 1303, 1739
Hpy166II	GTNNAC	8 cut(s)	119, 786, 1207, 1300, 1570, 1621, 1705, 1723
Hpy188I	TCNGA	5 cut(s)	385, 682, 1269, 1738, 1841
Hpy188III	TCNNGA	5 cut(s)	566, 614, 630, 1399, 1678
Hpy8I	GTNNAC	8 cut(s)	119, 786, 1207, 1300, 1570, 1621, 1705, 1723
HpyAV	CCTTC	6 cut(s)	157, 186, 284, 1105, 1208, 1606
HpyCH4III	ACNGT	2 cut(s)	1574, 1727
HpyCH4IV	ACGT	9 cut(s)	552, 1215, 1283, 1379, 1466, 1485, 1566, 1731, 1896
HpyCH4V	TGCA	8 cut(s)	447, 468, 636, 645, 1116, 1179, 1312, 1339
HpyF3I	CTNAG	5 cut(s)	28, 55, 406, 612, 1758
HpySE526I	ACGT	9 cut(s)	552, 1215, 1283, 1379, 1466, 1485, 1566, 1731, 1896
Hsp92I	GRCGYC	4 cut(s)	1074, 1466, 1508, 1806
Hsp92II	CATG	3 cut(s)	81, 503, 1324
HspAI	GCGC	2 cut(s)	1074, 1649
KasI	GGCGCC	1 cut(s)	1073
Ksp22I	TGATCA	1 cut(s)	738
KspI	CCGCGG	1 cut(s)	1072
Kzo9I	GATC	6 cut(s)	36, 347, 509, 588, 738, 1836
LguI	GCTCTTC	1 cut(s)	933
LmnI	GCTCC	7 cut(s)	395, 401, 1481, 1594, 1642, 1695, 1825
Lsp1109I	GCAGC	4 cut(s)	152, 459, 527, 1191
LweI	GCATC	4 cut(s)	244, 623, 1091, 1738
MabI	ACCWGGT	1 cut(s)	1695
MaeI	CTAG	1 cut(s)	1202
MaeII	ACGT	9 cut(s)	552, 1215, 1283, 1379, 1466, 1485, 1566, 1731, 1896
MaeIII	GTNAC	5 cut(s)	526, 1514, 1562, 1727, 1897
MalI	GATC	6 cut(s)	38, 349, 511, 590, 740, 1838
MboI	GATC	6 cut(s)	36, 347, 509, 588, 738, 1836
MboII	GAAGA	9 cut(s)	144, 147, 258, 324, 428, 823, 881, 950, 1384
MflI	RGATCY	1 cut(s)	509
MhlI	GDGCHC	1 cut(s)	945
MluCI	AATT	4 cut(s)	310, 582, 695, 1153
Mly113I	GGCGCC	1 cut(s)	1074
MlyI	GAGTC	1 cut(s)	611
MmeI	TCCRAC	5 cut(s)	408, 675, 705, 1125, 1761
MroXI	GAANNNNTTC	2 cut(s)	1094, 1391
MseI	TTAA	5 cut(s)	299, 672, 791, 848, 1416
MslI	CAYNNNNRTG	1 cut(s)	1325
MspA1I	CMGCKG	2 cut(s)	1071, 1627
MspI	CCGG	9 cut(s)	1086, 1479, 1494, 1512, 1526, 1537, 1709, 1802, 1864
Mva1269I	GAATGC	1 cut(s)	470
MvaI	CCWGG	4 cut(s)	162, 536, 1441, 1697
MvnI	CGCG	1 cut(s)	1071
NarI	GGCGCC	1 cut(s)	1074
NciI	CCSGG	7 cut(s)	1087, 1480, 1512, 1526, 1709, 1864, 1865
NcoI	CCATGG	1 cut(s)	1320
NdeII	GATC	6 cut(s)	36, 347, 509, 588, 738, 1836
NlaIII	CATG	3 cut(s)	81, 503, 1324
NlaIV	GGNNCC	6 cut(s)	391, 1075, 1228, 1252, 1353, 1524
NmeAIII	GCCGAG	1 cut(s)	833
NmuCI	GTSAC	4 cut(s)	526, 1562, 1727, 1897
NspV	TTCGAA	2 cut(s)	376, 1877
PaqCI	CACCTGC	1 cut(s)	1279
PceI	AGGCCT	1 cut(s)	1194
PciSI	GCTCTTC	1 cut(s)	933
PcsI	WCGNNNNNNNCGW	3 cut(s)	137, 942, 1728
PctI	GAATGC	1 cut(s)	470
PdmI	GAANNNNTTC	2 cut(s)	1094, 1391
PfeI	GAWTC	4 cut(s)	433, 757, 762, 947
PflFI	GACNNNGTC	1 cut(s)	1468
PfoI	TCCNGGA	1 cut(s)	1085
PinAI	ACCGGT	2 cut(s)	1493, 1536
PleI	GAGTC	1 cut(s)	611
PluTI	GGCGCC	1 cut(s)	1077
PpsI	GAGTC	1 cut(s)	611
Psp124BI	GAGCTC	1 cut(s)	945
Psp6I	CCWGG	4 cut(s)	160, 534, 1439, 1695
PspFI	CCCAGC	1 cut(s)	341
PspGI	CCWGG	4 cut(s)	160, 534, 1439, 1695
PspN4I	GGNNCC	6 cut(s)	391, 1075, 1228, 1252, 1353, 1524
PspPI	GGNCC	8 cut(s)	837, 1250, 1363, 1491, 1496, 1522, 1621, 1684
PsrI	GAACNNNNNNTAC	2 cut(s)	1695, 1727
PstI	CTGCAG	2 cut(s)	449, 1181
PstNI	CAGNNNCTG	1 cut(s)	453
PsuI	RGATCY	1 cut(s)	509
PsyI	GACNNNGTC	1 cut(s)	1468
RsaI	GTAC	3 cut(s)	1334, 1484, 1571
RsaNI	GTAC	3 cut(s)	1333, 1483, 1570
RseI	CAYNNNNRTG	1 cut(s)	1325
Rsr2I	CGGWCCG	1 cut(s)	1496
RsrII	CGGWCCG	1 cut(s)	1496
SacI	GAGCTC	1 cut(s)	945
SacII	CCGCGG	1 cut(s)	1072
SalI	GTCGAC	2 cut(s)	1298, 1721
SapI	GCTCTTC	1 cut(s)	933
SaqAI	TTAA	5 cut(s)	299, 672, 791, 848, 1416
Sau3AI	GATC	6 cut(s)	36, 347, 509, 588, 738, 1836
Sau96I	GGNCC	8 cut(s)	837, 1250, 1363, 1491, 1496, 1522, 1621, 1684
SchI	GAGTC	1 cut(s)	611
SduI	GDGCHC	1 cut(s)	945
SexAI	ACCWGGT	1 cut(s)	1695
SfaNI	GCATC	4 cut(s)	244, 623, 1091, 1738
SfcI	CTRYAG	2 cut(s)	445, 1177
SfoI	GGCGCC	1 cut(s)	1075
Sfr303I	CCGCGG	1 cut(s)	1072
SfuI	TTCGAA	2 cut(s)	376, 1877
SgrBI	CCGCGG	1 cut(s)	1072
SgrDI	CGTCGACG	1 cut(s)	1721
SinI	GGWCC	6 cut(s)	837, 1363, 1491, 1496, 1522, 1621
SmaI	CCCGGG	1 cut(s)	1865
SmiMI	CAYNNNNRTG	1 cut(s)	1325
SmlI	CTYRAG	2 cut(s)	413, 488
SmoI	CTYRAG	2 cut(s)	413, 488
Sse9I	AATT	4 cut(s)	310, 582, 695, 1153
SseBI	AGGCCT	1 cut(s)	1194
SspDI	GGCGCC	1 cut(s)	1073
SspMI	CTAG	1 cut(s)	1202
SstI	GAGCTC	1 cut(s)	945
StuI	AGGCCT	1 cut(s)	1194
StyI	CCWWGG	3 cut(s)	458, 1320, 1809
TaaI	ACNGT	2 cut(s)	1574, 1727
TaiI	ACGT	9 cut(s)	555, 1218, 1286, 1382, 1469, 1488, 1569, 1734, 1899
TaqII	GACCGA	1 cut(s)	1568
TasI	AATT	4 cut(s)	310, 582, 695, 1153
TatI	WGTACW	1 cut(s)	1569
TfiI	GAWTC	4 cut(s)	433, 757, 762, 947
Tru1I	TTAA	5 cut(s)	299, 672, 791, 848, 1416
Tru9I	TTAA	5 cut(s)	299, 672, 791, 848, 1416
TscAI	CASTG	2 cut(s)	458, 1365
TseFI	GTSAC	4 cut(s)	526, 1562, 1727, 1897
TseI	GCWGC	4 cut(s)	165, 447, 540, 1179
Tsp45I	GTSAC	4 cut(s)	526, 1562, 1727, 1897
TspDTI	ATGAA	1 cut(s)	1384
TspGWI	ACGGA	1 cut(s)	7
TspMI	CCCGGG	1 cut(s)	1863
TspRI	CASTG	2 cut(s)	458, 1365
Tth111I	GACNNNGTC	1 cut(s)	1468
VpaK11BI	GGWCC	6 cut(s)	837, 1363, 1491, 1496, 1522, 1621
XapI	RAATTY	1 cut(s)	1153
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1816
XmaI	CCCGGG	1 cut(s)	1863
XmiI	GTMKAC	4 cut(s)	1206, 1299, 1704, 1722
XmnI	GAANNNNTTC	2 cut(s)	1094, 1391
XspI	CTAG	1 cut(s)	1202
ZraI	GACGTC	1 cut(s)	1467

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.