Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0012229

Physical Location & Seq

Genomic Coordinates Reverse (-)

57490135 .. 57492060

Gene Structure 1926 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG433000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1926 bp

ATGCCTCCGTCGCTTGCTGCCTGTGTCTTCTTCTTCTTCTCAGTTCTTGGCCTCATTTCCTCAGTCCACTCGCACTTATGCTCACCGCACCATCAATCTCTATGCCCACCCTTTACTTCAACTCCTCCTTTTCCCTTCTCTTTCTCACCTGGCTGTGGCCACCCCTCTTTCCAAATCAAATGCTACGCAAATCACTCCATAATCACCATTAACAACCTCGCTTTCTCCCTCCTCCACTACGAGCCCAACTCCACATCTCTCCTTCTCTCCCCCTACATCACCAACTCGACAATTAAAGCTAAATCCCTTAGCACTAGCACTAGCAACTGCTCATCCCCTCACTTTCTCTCAATCCCCAGCCGCTCCATCAACCTCTCCTCTTCCCCCTTTCGCTTCTCCGACGGTTCCTGTAACCGCCTCTCTGTTCTCAAACCTTGCTCCCCGCCAAATCTTCCTAATTGCAGCCATTGTCCCTGGGAATGCAAGCTCATAAAGAGCCCGGTCAAGCTACTCCCTGGCTGTGGATCTAGTCGCAGCTCTGTTCCCAACCAGGGCTGCCAAGACGATGTCTTGGGATATCTTGACAACTTCCTCCAACTCGGGATCGAGCTCGAGTGGGACGAAGCTCAAGATTCTTACTTGTCAAGCTGCAGAGATTGCGAAGCCAACAGCGGCATATGCGGCTTCAATTCCTCCGACCCATCACGGCAATTCCTGTGTCTCCACACCAAAGCTCAACTCTCACCCCCATGGATTAGCAAGCACAACCCCCATCGCGTCGCCATTTTGTGCTCGGTCTTGGCAGTGACTTGTTTGATGGTTATGGCTTCTGTTTCTTTGGCCATAATCAGGTCCAGAAGAAGACTACACTCCTCCGCCACAGAAGAAGACCCGACCACATTGTTCCTCCAGCGCCACCGCTCCGCCAGTCTCCTCCCGCCGGTATTCACCTACGAAGAGCTGGAGTCATCCACCAACAAATTCGACACCAAGCGCAAAATCGGCGACGGCGGGTTCGGGTCAGTCTACCTGGGCCAGCTCTACGACAGCCGCGTCGTCGCCGTAAAGTACCTCCACAAGCCCCACCACTCCGCCGCCTCCGGCAAGGCCTTCTCCAACAAGTGCTTCTGCAACGAAATCCTAATCCTCTCCTCAATTGACCATCCTAACCTCGTCAAGCTCCACGGCTACTGCAGCGATCCCAGGGGCCTTCTGTTGGTCTATGATTACGTCCCCAACGGCACTCTCGCCGACCATCTCCACGGCCCGAAGAGCCTCTACCGGAAAGGGTCGATGACGTGGCAGGTGAGGGTTGATATAGCTTTGCAAACCGCCATGGTTATGGAGTACTTGCATTTCTCGGTGGTGCCTCCGGTGGTTCACAGAGACATAACTTCGTCTAATATTTTCGTGGAAAGAGACATGAGAATTAAAGTCGGGGATTTCGGGCTGTCGAGGCTTTTGGTCTTCCCGGAAACGACGTCGTCGAGTTCGGGGTACGTTTGGACCGGGCCTCAGGGTACTCCGGGTTACCTGGACCCGGATTACCACCGGTCCTTCCGGTTGACGGATAAGAGTGACGTGTACAGCTTCGGGGTGGTGCTGCTGGAGTTGATTTCGGGTCAGAAGGCGGTGGACCAGAGGAGGGACAAGAGGGAGTTGGCTTTGGCGGATATGATGGTGTCCAAGATCCAGATGGGCCTGCTCCATCAGGTAGTCGACCCGGTTCTGATTGTGGATGGGAATGTGACGGATGGTGTTGACGTGGCGGCAGAGCTGGCGTTCAGGTGCGTCGCGGCGGAGAAGGACGACCGTCCAGATGCGAGGGAGGTGGTGCAGGAGCTGAGGCGGATCAGGGGCCGTACACGTGGGATTAGCCGCGCTTCGAGTTCGAATGTGATTGGTGCTGACGTGGCAAAAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

641

Amino Acids

70.65

Weight (kDa)

8.35

Isoelectric Point (pI)

45.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WAK_assoc	PF14380	190 - 242	8.7e-06	Wall-associated receptor kinase C-terminal
Pkinase	PF00069	329 - 613	1.2e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	330 - 551	2.1e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69910
fragaria_vesca	FvH4_4g35620
malus_domestica	MD13G1081800.v1.1 MD16G1080900.v1.1
prunus_persica	Prupe.1G265100_v2.0.a1
pyrus_communis	pycom13g07060 pycom16g06990
rosa_chinensis	RchiOBHm_Chr4g0445241
rosa_laevigata	RLG00000005756
rosa_multiflora	Rmu_co8464497.1_g000001 Rmu_sc0000050.1_g000028 Rmu_sc0000050.1_g000029
rosa_roxburghii	Rroxscaffold_5G00385740
rosa_rugosa	Rorug04G0360000
rosa_samantha	Rh4AG421100 Rh4BG433000 Rh4CG447600 Rh4DG429000
rosa_wichuraiana	Rw4G036110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1294
AasI	GACNNNNNNGTC	1 cut(s)	1052
AatII	GACGTC	1 cut(s)	1484
Acc36I	ACCTGC	1 cut(s)	1294
AccB1I	GGYRCC	1 cut(s)	1366
AccBSI	CCGCTC	2 cut(s)	363, 921
AccI	GTMKAC	2 cut(s)	1026, 1719
AccII	CGCG	4 cut(s)	777, 1053, 1796, 1881
AclWI	GGATC	5 cut(s)	532, 611, 1193, 1684, 1859
AcoI	YGGCCR	2 cut(s)	157, 840
AcsI	RAATTY	1 cut(s)	980
AcvI	CACGTG	1 cut(s)	1868
AcyI	GRCGYC	1 cut(s)	1481
AfaI	GTAC	6 cut(s)	1070, 1349, 1499, 1522, 1586, 1864
AfiI	CCNNNNNNNGG	9 cut(s)	155, 521, 551, 849, 940, 1216, 1540, 1567, 1645
AflIII	ACRYGT	2 cut(s)	1581, 1865
AgeI	ACCGGT	1 cut(s)	1551
AgsI	TTSAA	2 cut(s)	120, 688
AjiI	CACGTC	4 cut(s)	1299, 1582, 1765, 1912
AjnI	CCWGG	7 cut(s)	148, 473, 514, 549, 1029, 1202, 1533
AjuI	GAANNNNNNNTTGG	2 cut(s)	1109, 1141
Alw21I	GWGCWC	2 cut(s)	612, 794
Alw26I	GTCTC	4 cut(s)	725, 935, 1380, 1413
AlwI	GGATC	5 cut(s)	532, 611, 1193, 1684, 1859
AlwNI	CAGNNNCTG	1 cut(s)	1843
Ama87I	CYCGRG	2 cut(s)	599, 611
ApeKI	GCWGC	7 cut(s)	17, 462, 534, 555, 648, 1194, 1603
ApoI	RAATTY	1 cut(s)	980
ArsI	GACNNNNNNTTYG	2 cut(s)	998, 1030
AsiGI	ACCGGT	1 cut(s)	1551
AspLEI	GCGC	3 cut(s)	915, 996, 1883
AsuC2I	CCSGG	6 cut(s)	500, 1472, 1510, 1527, 1541, 1724
AsuHPI	GGTGA	7 cut(s)	75, 138, 196, 271, 735, 940, 1318
AsuII	TTCGAA	1 cut(s)	1892
AvaI	CYCGRG	2 cut(s)	599, 611
AvaII	GGWCC	5 cut(s)	852, 1506, 1537, 1554, 1636
AxyI	CCTNAGG	1 cut(s)	1515
BalI	TGGCCA	2 cut(s)	159, 842
BanI	GGYRCC	1 cut(s)	1366
BanII	GRGCYC	3 cut(s)	246, 500, 612
BarI	GAAGNNNNNNTAC	2 cut(s)	1262, 1294
BbrPI	CACGTG	1 cut(s)	1868
BbsI	GAAGAC	4 cut(s)	19, 868, 894, 1459
Bbv12I	GWGCWC	2 cut(s)	612, 794
BbvCI	CCTCAGC	1 cut(s)	1844
BbvI	GCAGC	7 cut(s)	4, 474, 542, 546, 635, 1206, 1590
BceAI	ACGGC	7 cut(s)	722, 1024, 1046, 1201, 1255, 1279, 1845
BciT130I	CCWGG	7 cut(s)	150, 475, 516, 551, 1031, 1204, 1535
BcnI	CCSGG	6 cut(s)	500, 1472, 1510, 1527, 1541, 1724
BcoDI	GTCTC	4 cut(s)	725, 935, 1380, 1413
BfaI	CTAG	3 cut(s)	315, 321, 528
BfmI	CTRYAG	2 cut(s)	649, 1192
BfoI	RGCGCY	1 cut(s)	916
BfuAI	ACCTGC	1 cut(s)	1294
BmcAI	AGTACT	1 cut(s)	1349
Bme18I	GGWCC	5 cut(s)	852, 1506, 1537, 1554, 1636
BmeT110I	CYCGRG	2 cut(s)	599, 611
BmgBI	CACGTC	4 cut(s)	1299, 1582, 1765, 1912
BmiI	GGNNCC	5 cut(s)	406, 1208, 1368, 1539, 1859
BmsI	GCATC	1 cut(s)	1810
BoxI	GACNNNNGTC	1 cut(s)	1812
BpiI	GAAGAC	4 cut(s)	19, 868, 894, 1459
BplI	GAGNNNNNCTC	2 cut(s)	233, 265
BpmI	CTGGAG	3 cut(s)	893, 983, 1628
Bpu10I	CCTNAGC	2 cut(s)	308, 1844
Bpu14I	TTCGAA	1 cut(s)	1892
BpuEI	CTTGAG	1 cut(s)	612
BpuMI	CCSGG	6 cut(s)	500, 1472, 1510, 1527, 1541, 1724
BsaAI	YACGTR	1 cut(s)	1868
BsaHI	GRCGYC	1 cut(s)	1481
BsaWI	WCCGGW	4 cut(s)	1281, 1372, 1551, 1560
BsaXI	ACNNNNNCTCC	2 cut(s)	106, 136
Bsc4I	CCNNNNNNNGG	9 cut(s)	155, 521, 551, 849, 940, 1216, 1540, 1567, 1645
Bse118I	RCCGGY	2 cut(s)	940, 1551
Bse1I	ACTGG	1 cut(s)	927
Bse21I	CCTNAGG	1 cut(s)	1515
BseBI	CCWGG	7 cut(s)	150, 475, 516, 551, 1031, 1204, 1535
BseGI	GGATG	5 cut(s)	332, 968, 1162, 1744, 1759
BseLI	CCNNNNNNNGG	9 cut(s)	155, 521, 551, 849, 940, 1216, 1540, 1567, 1645
BseMII	CTCAG	4 cut(s)	54, 75, 1529, 1835
BseNI	ACTGG	1 cut(s)	927
BseRI	GAGGAG	7 cut(s)	114, 221, 367, 862, 923, 1141, 1657
BseXI	GCAGC	7 cut(s)	4, 474, 542, 546, 635, 1206, 1590
BseYI	CCCAGC	1 cut(s)	356
BsgI	GTGCAG	1 cut(s)	1856
Bsh1236I	CGCG	4 cut(s)	777, 1053, 1796, 1881
Bsh1285I	CGRYCG	1 cut(s)	1813
BshNI	GGYRCC	1 cut(s)	1366
BshTI	ACCGGT	1 cut(s)	1551
BsiEI	CGRYCG	1 cut(s)	1813
BsiHKAI	GWGCWC	2 cut(s)	612, 794
BsiHKCI	CYCGRG	2 cut(s)	599, 611
BslFI	GGGAC	4 cut(s)	456, 632, 1217, 1661
BslI	CCNNNNNNNGG	9 cut(s)	155, 521, 551, 849, 940, 1216, 1540, 1567, 1645
BsmAI	GTCTC	4 cut(s)	725, 935, 1380, 1413
BsmFI	GGGAC	4 cut(s)	456, 632, 1217, 1661
BsmI	GAATGC	1 cut(s)	485
BsoBI	CYCGRG	2 cut(s)	599, 611
Bsp119I	TTCGAA	1 cut(s)	1892
Bsp1286I	GDGCHC	4 cut(s)	246, 500, 612, 794
Bsp1407I	TGTACA	1 cut(s)	1584
Bsp143I	GATC	5 cut(s)	524, 603, 1198, 1689, 1851
Bsp19I	CCATGG	2 cut(s)	749, 1335
BspCNI	CTCAG	4 cut(s)	53, 74, 1528, 1836
BspFNI	CGCG	4 cut(s)	777, 1053, 1796, 1881
BspLI	GGNNCC	5 cut(s)	406, 1208, 1368, 1539, 1859
BspMAI	CTGCAG	2 cut(s)	653, 1196
BspMI	ACCTGC	1 cut(s)	1294
BspPI	GGATC	5 cut(s)	532, 611, 1193, 1684, 1859
BspQI	GCTCTTC	2 cut(s)	951, 1265
BspT104I	TTCGAA	1 cut(s)	1892
BspT107I	GGYRCC	1 cut(s)	1366
BsrBI	CCGCTC	2 cut(s)	363, 921
BsrFI	RCCGGY	2 cut(s)	940, 1551
BsrGI	TGTACA	1 cut(s)	1584
BsrI	ACTGG	1 cut(s)	927
BssAI	RCCGGY	2 cut(s)	940, 1551
BssMI	GATC	5 cut(s)	524, 603, 1198, 1689, 1851
BssNI	GRCGYC	1 cut(s)	1481
BssT1I	CCWWGG	2 cut(s)	749, 1335
Bst2UI	CCWGG	7 cut(s)	150, 475, 516, 551, 1031, 1204, 1535
Bst4CI	ACNGT	2 cut(s)	404, 1814
Bst6I	CTCTTC	3 cut(s)	385, 951, 1265
BstACI	GRCGYC	1 cut(s)	1481
BstAPI	GCANNNNNTGC	1 cut(s)	657
BstAUI	TGTACA	1 cut(s)	1584
BstBAI	YACGTR	1 cut(s)	1868
BstBI	TTCGAA	1 cut(s)	1892
BstC8I	GCNNGC	6 cut(s)	15, 485, 761, 1037, 1703, 1779
BstDEI	CTNAG	5 cut(s)	40, 61, 308, 1515, 1844
BstDSI	CCRYGG	4 cut(s)	749, 1183, 1261, 1335
BstEII	GGTNACC	1 cut(s)	1529
BstF5I	GGATG	5 cut(s)	332, 968, 1162, 1744, 1759
BstFNI	CGCG	4 cut(s)	777, 1053, 1796, 1881
BstH2I	RGCGCY	1 cut(s)	916
BstHHI	GCGC	3 cut(s)	915, 996, 1883
BstKTI	GATC	5 cut(s)	527, 606, 1201, 1692, 1854
BstMAI	GTCTC	4 cut(s)	725, 935, 1380, 1413
BstMBI	GATC	5 cut(s)	524, 603, 1198, 1689, 1851
BstMCI	CGRYCG	1 cut(s)	1813
BstNI	CCWGG	7 cut(s)	150, 475, 516, 551, 1031, 1204, 1535
BstPAI	GACNNNNGTC	1 cut(s)	1812
BstPI	GGTNACC	1 cut(s)	1529
BstSFI	CTRYAG	2 cut(s)	649, 1192
BstUI	CGCG	4 cut(s)	777, 1053, 1796, 1881
BstV1I	GCAGC	7 cut(s)	4, 474, 542, 546, 635, 1206, 1590
BstV2I	GAAGAC	4 cut(s)	19, 868, 894, 1459
BstX2I	RGATCY	2 cut(s)	524, 1689
BstXI	CCANNNNNNTGG	1 cut(s)	1342
BstYI	RGATCY	2 cut(s)	524, 1689
Bsu36I	CCTNAGG	1 cut(s)	1515
BtgI	CCRYGG	4 cut(s)	749, 1183, 1261, 1335
BtgZI	GCGATG	1 cut(s)	758
BtrI	CACGTC	4 cut(s)	1299, 1582, 1765, 1912
BtsCI	GGATG	5 cut(s)	332, 968, 1162, 1744, 1759
BtsI	GCAGTG	1 cut(s)	810
BtsIMutI	CAGTG	1 cut(s)	810
BveI	ACCTGC	1 cut(s)	1294
Cac8I	GCNNGC	6 cut(s)	15, 485, 761, 1037, 1703, 1779
CaiI	CAGNNNCTG	1 cut(s)	1843
CfoI	GCGC	3 cut(s)	915, 996, 1883
Cfr10I	RCCGGY	2 cut(s)	940, 1551
CseI	GACGC	3 cut(s)	766, 1042, 1780
Csp6I	GTAC	6 cut(s)	1069, 1348, 1498, 1521, 1585, 1863
CspAI	ACCGGT	1 cut(s)	1551
CviAII	CATG	3 cut(s)	750, 1336, 1423
CviQI	GTAC	6 cut(s)	1069, 1348, 1498, 1521, 1585, 1863
DdeI	CTNAG	5 cut(s)	40, 61, 308, 1515, 1844
DpnI	GATC	5 cut(s)	526, 605, 1200, 1691, 1853
DpnII	GATC	5 cut(s)	524, 603, 1198, 1689, 1851
DrdI	GACNNNNNNGTC	1 cut(s)	1052
DseDI	GACNNNNNNGTC	1 cut(s)	1052
EaeI	YGGCCR	2 cut(s)	157, 840
Eam1104I	CTCTTC	3 cut(s)	385, 951, 1265
EarI	CTCTTC	3 cut(s)	385, 951, 1265
EciI	GGCGGA	6 cut(s)	865, 913, 1081, 1685, 1814, 1864
Ecl136II	GAGCTC	1 cut(s)	610
Eco130I	CCWWGG	2 cut(s)	749, 1335
Eco147I	AGGCCT	1 cut(s)	1109
Eco24I	GRGCYC	3 cut(s)	246, 500, 612
Eco32I	GATATC	1 cut(s)	578
Eco47I	GGWCC	5 cut(s)	852, 1506, 1537, 1554, 1636
Eco53kI	GAGCTC	1 cut(s)	610
Eco72I	CACGTG	1 cut(s)	1868
Eco81I	CCTNAGG	1 cut(s)	1515
Eco88I	CYCGRG	2 cut(s)	599, 611
Eco91I	GGTNACC	1 cut(s)	1529
EcoICRI	GAGCTC	1 cut(s)	610
EcoO109I	RGGNCCY	1 cut(s)	1207
EcoO65I	GGTNACC	1 cut(s)	1529
EcoRII	CCWGG	7 cut(s)	148, 473, 514, 549, 1029, 1202, 1533
EcoRV	GATATC	1 cut(s)	578
EcoT14I	CCWWGG	2 cut(s)	749, 1335
EcoT38I	GRGCYC	3 cut(s)	246, 500, 612
ErhI	CCWWGG	2 cut(s)	749, 1335
FaeI	CATG	3 cut(s)	753, 1339, 1426
FaqI	GGGAC	4 cut(s)	456, 632, 1217, 1661
FatI	CATG	3 cut(s)	749, 1335, 1422
FauI	CCCGC	3 cut(s)	450, 945, 1004
FauNDI	CATATG	1 cut(s)	677
FblI	GTMKAC	2 cut(s)	1026, 1719
FokI	GGATG	5 cut(s)	319, 955, 1149, 1751, 1766
FriOI	GRGCYC	3 cut(s)	246, 500, 612
FspBI	CTAG	3 cut(s)	315, 321, 528
GlaI	GCGC	3 cut(s)	914, 995, 1882
GsaI	CCCAGC	1 cut(s)	360
GsuI	CTGGAG	3 cut(s)	893, 983, 1628
HaeII	RGCGCY	1 cut(s)	916
HgaI	GACGC	3 cut(s)	766, 1042, 1780
HhaI	GCGC	3 cut(s)	915, 996, 1883
Hin1I	GRCGYC	1 cut(s)	1481
Hin1II	CATG	3 cut(s)	753, 1339, 1426
Hin6I	GCGC	3 cut(s)	913, 994, 1881
HinP1I	GCGC	3 cut(s)	913, 994, 1881
HincII	GTYRAC	3 cut(s)	1566, 1720, 1762
HindII	GTYRAC	3 cut(s)	1566, 1720, 1762
HinfI	GANTC	2 cut(s)	632, 965
HphI	GGTGA	7 cut(s)	75, 138, 196, 271, 735, 940, 1318
Hpy166II	GTNNAC	9 cut(s)	67, 1027, 1381, 1566, 1585, 1636, 1720, 1762, 1865
Hpy188I	TCNGA	4 cut(s)	400, 697, 1626, 1731
Hpy188III	TCNNGA	6 cut(s)	581, 601, 629, 855, 1693, 1817
Hpy8I	GTNNAC	9 cut(s)	67, 1027, 1381, 1566, 1585, 1636, 1720, 1762, 1865
HpyAV	CCTTC	7 cut(s)	145, 272, 1120, 1220, 1567, 1621, 1798
HpyCH4III	ACNGT	2 cut(s)	404, 1814
HpyCH4IV	ACGT	8 cut(s)	1230, 1298, 1481, 1500, 1581, 1764, 1867, 1911
HpyCH4V	TGCA	8 cut(s)	462, 483, 651, 1131, 1194, 1327, 1354, 1837
HpyF3I	CTNAG	5 cut(s)	40, 61, 308, 1515, 1844
HpySE526I	ACGT	8 cut(s)	1230, 1298, 1481, 1500, 1581, 1764, 1867, 1911
Hsp92I	GRCGYC	1 cut(s)	1481
Hsp92II	CATG	3 cut(s)	753, 1339, 1426
HspAI	GCGC	3 cut(s)	913, 994, 1881
Kzo9I	GATC	5 cut(s)	524, 603, 1198, 1689, 1851
LguI	GCTCTTC	2 cut(s)	951, 1265
LmnI	GCTCC	6 cut(s)	368, 443, 926, 1185, 1710, 1840
Lsp1109I	GCAGC	7 cut(s)	4, 474, 542, 546, 635, 1206, 1590
LweI	GCATC	1 cut(s)	1810
MaeI	CTAG	3 cut(s)	315, 321, 528
MaeII	ACGT	8 cut(s)	1230, 1298, 1481, 1500, 1581, 1764, 1867, 1911
MaeIII	GTNAC	5 cut(s)	410, 805, 1529, 1577, 1747
MalI	GATC	5 cut(s)	526, 605, 1200, 1691, 1853
MbiI	CCGCTC	2 cut(s)	363, 921
MboI	GATC	5 cut(s)	524, 603, 1198, 1689, 1851
MfeI	CAATTG	1 cut(s)	1155
MflI	RGATCY	2 cut(s)	524, 1689
MhlI	GDGCHC	4 cut(s)	246, 500, 612, 794
MlsI	TGGCCA	2 cut(s)	159, 842
MluCI	AATT	7 cut(s)	291, 457, 688, 710, 980, 1155, 1428
MluNI	TGGCCA	2 cut(s)	159, 842
MlyI	GAGTC	1 cut(s)	974
MmeI	TCCRAC	4 cut(s)	423, 619, 720, 1140
Mox20I	TGGCCA	2 cut(s)	159, 842
MscI	TGGCCA	2 cut(s)	159, 842
MseI	TTAA	3 cut(s)	210, 294, 1431
MslI	CAYNNNNRTG	2 cut(s)	748, 1340
Msp20I	TGGCCA	2 cut(s)	159, 842
MspA1I	CMGCKG	1 cut(s)	672
MunI	CAATTG	1 cut(s)	1155
Mva1269I	GAATGC	1 cut(s)	485
MvaI	CCWGG	7 cut(s)	150, 475, 516, 551, 1031, 1204, 1535
MvnI	CGCG	4 cut(s)	777, 1053, 1796, 1881
NciI	CCSGG	6 cut(s)	500, 1472, 1510, 1527, 1541, 1724
NcoI	CCATGG	2 cut(s)	749, 1335
NdeI	CATATG	1 cut(s)	677
NdeII	GATC	5 cut(s)	524, 603, 1198, 1689, 1851
NlaIII	CATG	3 cut(s)	753, 1339, 1426
NlaIV	GGNNCC	5 cut(s)	406, 1208, 1368, 1539, 1859
NmuCI	GTSAC	3 cut(s)	805, 1577, 1747
NspV	TTCGAA	1 cut(s)	1892
PaeR7I	CTCGAG	1 cut(s)	611
PaqCI	CACCTGC	1 cut(s)	1294
PasI	CCCWGGG	2 cut(s)	474, 1203
PceI	AGGCCT	1 cut(s)	1109
PciSI	GCTCTTC	2 cut(s)	951, 1265
PcsI	WCGNNNNNNNCGW	3 cut(s)	618, 1050, 1484
PctI	GAATGC	1 cut(s)	485
PfeI	GAWTC	1 cut(s)	632
PflFI	GACNNNGTC	2 cut(s)	566, 1483
PfoI	TCCNGGA	1 cut(s)	1470
PinAI	ACCGGT	1 cut(s)	1551
PleI	GAGTC	1 cut(s)	973
PmaCI	CACGTG	1 cut(s)	1868
PmlI	CACGTG	1 cut(s)	1868
PpsI	GAGTC	1 cut(s)	973
Ppu21I	YACGTR	1 cut(s)	1868
PshAI	GACNNNNGTC	1 cut(s)	1812
Psp124BI	GAGCTC	1 cut(s)	612
Psp6I	CCWGG	7 cut(s)	148, 473, 514, 549, 1029, 1202, 1533
PspCI	CACGTG	1 cut(s)	1868
PspEI	GGTNACC	1 cut(s)	1529
PspFI	CCCAGC	1 cut(s)	356
PspGI	CCWGG	7 cut(s)	148, 473, 514, 549, 1029, 1202, 1533
PspN4I	GGNNCC	5 cut(s)	406, 1208, 1368, 1539, 1859
PspXI	VCTCGAGB	1 cut(s)	611
PstI	CTGCAG	2 cut(s)	653, 1196
PstNI	CAGNNNCTG	1 cut(s)	1843
PsuI	RGATCY	2 cut(s)	524, 1689
PsyI	GACNNNGTC	2 cut(s)	566, 1483
RsaI	GTAC	6 cut(s)	1070, 1349, 1499, 1522, 1586, 1864
RsaNI	GTAC	6 cut(s)	1069, 1348, 1498, 1521, 1585, 1863
RseI	CAYNNNNRTG	2 cut(s)	748, 1340
SacI	GAGCTC	1 cut(s)	612
SalI	GTCGAC	1 cut(s)	1718
SapI	GCTCTTC	2 cut(s)	951, 1265
SaqAI	TTAA	3 cut(s)	210, 294, 1431
Sau3AI	GATC	5 cut(s)	524, 603, 1198, 1689, 1851
ScaI	AGTACT	1 cut(s)	1349
SchI	GAGTC	1 cut(s)	974
SduI	GDGCHC	4 cut(s)	246, 500, 612, 794
SfaNI	GCATC	1 cut(s)	1810
SfcI	CTRYAG	2 cut(s)	649, 1192
Sfr274I	CTCGAG	1 cut(s)	611
SfuI	TTCGAA	1 cut(s)	1892
SinI	GGWCC	5 cut(s)	852, 1506, 1537, 1554, 1636
SlaI	CTCGAG	1 cut(s)	611
SmiMI	CAYNNNNRTG	2 cut(s)	748, 1340
SmlI	CTYRAG	2 cut(s)	611, 627
SmoI	CTYRAG	2 cut(s)	611, 627
Sse9I	AATT	7 cut(s)	291, 457, 688, 710, 980, 1155, 1428
SseBI	AGGCCT	1 cut(s)	1109
SspI	AATATT	1 cut(s)	1405
SspMI	CTAG	3 cut(s)	315, 321, 528
SstI	GAGCTC	1 cut(s)	612
StuI	AGGCCT	1 cut(s)	1109
StyI	CCWWGG	2 cut(s)	749, 1335
TaaI	ACNGT	2 cut(s)	404, 1814
TaiI	ACGT	8 cut(s)	1233, 1301, 1484, 1503, 1584, 1767, 1870, 1914
TaqII	GACCGA	1 cut(s)	784
TasI	AATT	7 cut(s)	291, 457, 688, 710, 980, 1155, 1428
TatI	WGTACW	2 cut(s)	1347, 1584
TauI	GCSGC	8 cut(s)	363, 675, 684, 1053, 1097, 1772, 1799, 1881
TfiI	GAWTC	1 cut(s)	632
Tru1I	TTAA	3 cut(s)	210, 294, 1431
Tru9I	TTAA	3 cut(s)	210, 294, 1431
TscAI	CASTG	1 cut(s)	810
TseFI	GTSAC	3 cut(s)	805, 1577, 1747
TseI	GCWGC	7 cut(s)	17, 462, 534, 555, 648, 1194, 1603
Tsp45I	GTSAC	3 cut(s)	805, 1577, 1747
TspGWI	ACGGA	2 cut(s)	1583, 1766
TspRI	CASTG	1 cut(s)	810
Tth111I	GACNNNGTC	2 cut(s)	566, 1483
VpaK11BI	GGWCC	5 cut(s)	852, 1506, 1537, 1554, 1636
XapI	RAATTY	1 cut(s)	980
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1693
XhoI	CTCGAG	1 cut(s)	611
XmiI	GTMKAC	2 cut(s)	1026, 1719
XspI	CTAG	3 cut(s)	315, 321, 528
ZraI	GACGTC	1 cut(s)	1482
ZrmI	AGTACT	1 cut(s)	1349

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.