MD16G1104600.v1.1

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010164
Physical Location & Seq
Forward (+)
7333031 .. 7338063
5033 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1104600.v1.1.491

Sequence Viewer

Length: 1704 bp
ATGAAGATTGAGTTTCCATATACTCTCAACATTCCTCCTCCCAACATGGCAAGGTCAATCATCTCATTTTTCTCCTTTCTCTCGATCCTTTCCCTTCTCACACTCACGCCCGCAGTCTCAGCTCTCCCACTCTCCACCTCCTCCCGATGGATCGTCGACGAGAGCGGGCAGCGCGTGAAGCTGGCCTGCGTCAATTGGGTGTCGCATCTCGACACCGTCGTAGCCGAAGGCCTCAGCAAGCAGCCGGTGGACGCAATCGCCAAGCTCATCGTGTCGCTGGGATTCAACTGCGTCAGGCTCACGTGGCCGCTTTTCCTGGGCACCAATGAGACGCTGGCGGCTGTCACTGTTCGGCAGTCGTTTCAGAGCCTCGGACTGCTCGATTCTATTTCCGGTATCCAGTCCAACAATCCCGCCCTCGTCGATCTCCCCCTCTTGAAAGCTTACCAGGCAGTGGTTTCTAGCCTTGGGAGCAACAATGTGATGGTTATACTGGACAATCACTTAAGCAAGCCTGGGTGGTGTTGCAGCAACACCGACGGCAATGGTTTCTTTGGCGACCAGTATTTCAACCCTGACCTTTGGATCACTGGCCTAACTCGAATGGCCACATTGTTCAAAGGCGTCAGCAATGTGGTCGGCATGAGCTTGAGGAATGAACTGCGAGGCCCCAAACAGAATGTTAACGATTGGTACAAGTACATGCAAAGAGGAGCTGAGGCAGTGCACTCGGCAAACGCAGACGTGCTTGTCATTCTCTCTGGCTTGAGTTTTGACACGGACTTATCTTTCCTCGCCAAACGGCCGGTGAACCTCACATTTGCCGGGAAAACAGTATTTGAAGTGCACTGGTATGGATTTTCAGACGGGCAGGCATGGCAGAGCGGCAATCCCAACCAAGTGTGCGGCCAAGTATACAATAACGTGAAGAGAAAGTCGGGATTTTTACTTGAAGAAGGGTTCCCATTGTTTGTGAGTGAGTTTGGGGCGGACCAAAGGGGAACCAATGTAAATGATAACAGGTACTTAAACTGCTTCATGGCGGCTGCTGCTGAACTTGATGTGGATTTTGCTTTGTGGACTCTGGTTGGGAGTTATTACTTTAGGGAAGGGGTGGTGGGTATGGAAGAGTATTATGGAATCTTGAACTGGGATTGGAGTGATATCAGGAATTCAAGCCTAACTCAGAGACTTTCTGTCCTCCAATCGCCTTTGCAAGGGCCAGGCTTATCTCAATCCAGAATGCACAAGATCATATTTCATCCAGCCACAGGCCTTTGCCTCCTAAAAGTTGGATTCCTTGGCCCCTTGAAGTTAGGTCCATGCTCTCAGTCTGGAGCCTGGACCTATTCATCGAGGAAGGTCCTAACGCTGAAAGGAACGTATTTCTGCATACAAGCTGACGAACTGAATAAGCCGGCAGCAGTGGGTATACTTTGTACTACCACGAATTCGCAATGGGATATCATTTCGGATTCTAAGTTGCACCTTCAATCTAAGACCACGGATGGCACTGAAGTTTGCCTTGATGTAGACTCTAGCAATACAGTTGTCACAAGTTCCTGCAAATGCTTGAGCAGAGATAATTCTTGCGACCCAGCAAGCCAGTGGTTCAAACTTGTCGACAGCACGATAAATTCGACACCTACAAGCCCCATTCTCGAGATCACCTCAGTCGTGCATATGATTGAGGCAACTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Cellulase

Protein Analysis

568

Amino Acids

62.24

Weight (kDa)

5.94

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cellulase PF00150 82 - 362 7.6e-23 Cellulase (glycosyl hydrolase family 5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 749
AccB1I GGYRCC 1 cut(s) 320
AccB7I CCANNNNNTGG 1 cut(s) 454
AccBSI CCGCTC 2 cut(s) 165, 885
AccI GTMKAC 5 cut(s) 156, 915, 1432, 1533, 1623
AccII CGCG 1 cut(s) 174
AciI CCGC 9 cut(s) 111, 165, 308, 338, 414, 885, 906, 989, 1043
AclWI GGATC 3 cut(s) 79, 158, 593
AcoI YGGCCR 4 cut(s) 305, 606, 803, 907
AcsI RAATTY 3 cut(s) 1171, 1450, 1636
AcuI CTGAAG 1 cut(s) 1536
AcvI CACGTG 1 cut(s) 303
AcyI GRCGYC 1 cut(s) 624
AfaI GTAC 4 cut(s) 695, 701, 1025, 1441
AfiI CCNNNNNNNGG 4 cut(s) 147, 454, 1217, 1271
AflII CTTAAG 1 cut(s) 505
AhdI GACNNNNNGTC 1 cut(s) 1196
AjiI CACGTC 1 cut(s) 745
AjnI CCWGG 5 cut(s) 315, 447, 514, 1222, 1340
AluBI AGCT 7 cut(s) 122, 181, 265, 443, 648, 716, 1400
AluI AGCT 7 cut(s) 122, 181, 265, 443, 648, 716, 1400
Alw21I GWGCWC 2 cut(s) 729, 849
Alw26I GTCTC 3 cut(s) 121, 323, 1183
Alw44I GTGCAC 2 cut(s) 725, 845
AlwI GGATC 3 cut(s) 79, 158, 593
Ama87I CYCGRG 1 cut(s) 1661
ApaLI GTGCAC 2 cut(s) 725, 845
ApeKI GCWGC 6 cut(s) 169, 241, 528, 1046, 1049, 1421
ApoI RAATTY 3 cut(s) 1171, 1450, 1636
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 7 cut(s) 668, 991, 1220, 1304, 1319, 1344, 1363
AsuC2I CCSGG 1 cut(s) 826
AsuHPI GGTGA 2 cut(s) 820, 1660
AvaI CYCGRG 1 cut(s) 1661
AvaII GGWCC 4 cut(s) 991, 1319, 1344, 1363
BaeGI GKGCMC 3 cut(s) 323, 729, 849
BalI TGGCCA 1 cut(s) 608
BanI GGYRCC 1 cut(s) 320
BbrPI CACGTG 1 cut(s) 303
Bbv12I GWGCWC 2 cut(s) 729, 849
BbvCI CCTCAGC 2 cut(s) 233, 717
BbvI GCAGC 6 cut(s) 181, 253, 540, 1033, 1036, 1433
BccI CCATC 3 cut(s) 141, 478, 1502
BceAI ACGGC 2 cut(s) 556, 818
BcgI CGANNNNNNTGC 4 cut(s) 619, 653, 711, 745
BciT130I CCWGG 5 cut(s) 317, 449, 516, 1224, 1342
BciVI GTATCC 1 cut(s) 407
BcnI CCSGG 1 cut(s) 826
BcoDI GTCTC 3 cut(s) 121, 323, 1183
BfaI CTAG 2 cut(s) 462, 1539
BfrI CTTAAG 1 cut(s) 505
BfuI GTATCC 1 cut(s) 407
Bme1390I CCNGG 6 cut(s) 317, 449, 516, 826, 1224, 1342
Bme18I GGWCC 4 cut(s) 991, 1319, 1344, 1363
BmeRI GACNNNNNGTC 1 cut(s) 1196
BmeT110I CYCGRG 1 cut(s) 1661
BmgBI CACGTC 1 cut(s) 745
BmgT120I GGNCC 7 cut(s) 668, 991, 1220, 1304, 1319, 1344, 1363
BmiI GGNNCC 6 cut(s) 322, 670, 962, 1003, 1306, 1339
BmrFI CCNGG 6 cut(s) 317, 449, 516, 826, 1224, 1342
BmrI ACTGGG 1 cut(s) 1159
BmsI GCATC 1 cut(s) 214
BmuI ACTGGG 1 cut(s) 1159
BplI GAGNNNNNCTC 2 cut(s) 1655, 1687
BpmI CTGGAG 1 cut(s) 1356
Bpu10I CCTNAGC 2 cut(s) 233, 717
BpuEI CTTGAG 3 cut(s) 670, 787, 1594
BpuMI CCSGG 1 cut(s) 826
BsaAI YACGTR 1 cut(s) 303
BsaHI GRCGYC 1 cut(s) 624
BsaJI CCNNGG 6 cut(s) 316, 370, 466, 515, 1300, 1503
BsaWI WCCGGW 1 cut(s) 392
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 454, 1217, 1271
Bse118I RCCGGY 3 cut(s) 244, 805, 1417
Bse1I ACTGG 7 cut(s) 400, 498, 562, 595, 854, 1154, 1606
Bse3DI GCAATG 3 cut(s) 550, 637, 1463
BseBI CCWGG 5 cut(s) 317, 449, 516, 1224, 1342
BseDI CCNNGG 6 cut(s) 316, 370, 466, 515, 1300, 1503
BseGI GGATG 2 cut(s) 1261, 1513
BseLI CCNNNNNNNGG 4 cut(s) 147, 454, 1217, 1271
BseMI GCAATG 3 cut(s) 550, 637, 1463
BseMII CTCAG 6 cut(s) 132, 247, 708, 1199, 1343, 1686
BseNI ACTGG 7 cut(s) 400, 498, 562, 595, 854, 1154, 1606
BseRI GAGGAG 3 cut(s) 27, 130, 726
BseSI GKGCMC 3 cut(s) 323, 729, 849
BseX3I CGGCCG 1 cut(s) 803
BseXI GCAGC 6 cut(s) 181, 253, 540, 1033, 1036, 1433
BseYI CCCAGC 2 cut(s) 277, 1597
Bsh1236I CGCG 1 cut(s) 174
Bsh1285I CGRYCG 1 cut(s) 806
BshNI GGYRCC 1 cut(s) 320
BsiEI CGRYCG 1 cut(s) 806
BsiHKAI GWGCWC 2 cut(s) 729, 849
BsiHKCI CYCGRG 1 cut(s) 1661
BsiSI CCGG 5 cut(s) 245, 393, 806, 825, 1418
BslI CCNNNNNNNGG 4 cut(s) 147, 454, 1217, 1271
BsmAI GTCTC 3 cut(s) 121, 323, 1183
BsmBI CGTCTC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 1248
BsoBI CYCGRG 1 cut(s) 1661
Bsp1286I GDGCHC 3 cut(s) 323, 729, 849
Bsp143I GATC 6 cut(s) 84, 150, 424, 585, 1251, 1665
BspACI CCGC 9 cut(s) 111, 165, 308, 338, 414, 885, 906, 989, 1043
BspCNI CTCAG 6 cut(s) 131, 246, 709, 1198, 1342, 1685
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 6 cut(s) 322, 670, 962, 1003, 1306, 1339
BspPI GGATC 3 cut(s) 79, 158, 593
BspT107I GGYRCC 1 cut(s) 320
BspTI CTTAAG 1 cut(s) 505
BsrBI CCGCTC 2 cut(s) 165, 885
BsrDI GCAATG 3 cut(s) 550, 637, 1463
BsrFI RCCGGY 3 cut(s) 244, 805, 1417
BsrI ACTGG 7 cut(s) 400, 498, 562, 595, 854, 1154, 1606
BssAI RCCGGY 3 cut(s) 244, 805, 1417
BssECI CCNNGG 6 cut(s) 316, 370, 466, 515, 1300, 1503
BssMI GATC 6 cut(s) 84, 150, 424, 585, 1251, 1665
BssNAI GTATAC 2 cut(s) 916, 1433
BssNI GRCGYC 1 cut(s) 624
BssT1I CCWWGG 2 cut(s) 466, 1300
Bst1107I GTATAC 2 cut(s) 916, 1433
Bst2UI CCWGG 5 cut(s) 317, 449, 516, 1224, 1342
Bst4CI ACNGT 4 cut(s) 217, 349, 835, 1549
Bst6I CTCTTC 2 cut(s) 923, 1122
BstACI GRCGYC 1 cut(s) 624
BstAFI CTTAAG 1 cut(s) 505
BstBAI YACGTR 1 cut(s) 303
BstDEI CTNAG 8 cut(s) 118, 233, 717, 1185, 1329, 1479, 1497, 1672
BstDSI CCRYGG 1 cut(s) 1503
BstENI CCTNNNNNAGG 1 cut(s) 1215
BstF5I GGATG 2 cut(s) 1261, 1513
BstFNI CGCG 1 cut(s) 174
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 6 cut(s) 87, 153, 427, 588, 1254, 1668
BstMAI GTCTC 3 cut(s) 121, 323, 1183
BstMBI GATC 6 cut(s) 84, 150, 424, 585, 1251, 1665
BstMCI CGRYCG 1 cut(s) 806
BstMWI GCNNNNNNNGC 8 cut(s) 119, 171, 178, 304, 449, 471, 877, 1049
BstNI CCWGG 5 cut(s) 317, 449, 516, 1224, 1342
BstNSI RCATGY 1 cut(s) 706
BstSCI CCNGG 6 cut(s) 315, 447, 514, 824, 1222, 1340
BstSLI GKGCMC 3 cut(s) 323, 729, 849
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 6 cut(s) 181, 253, 540, 1033, 1036, 1433
BstZ17I GTATAC 2 cut(s) 916, 1433
BstZI CGGCCG 1 cut(s) 803
BsuI GTATCC 1 cut(s) 407
BtgI CCRYGG 1 cut(s) 1503
BtrI CACGTC 1 cut(s) 745
BtsCI GGATG 2 cut(s) 1261, 1513
BtsI GCAGTG 3 cut(s) 459, 729, 1431
BtsIMutI CAGTG 8 cut(s) 345, 459, 588, 729, 847, 1431, 1512, 1613
CfoI GCGC 1 cut(s) 174
Cfr10I RCCGGY 3 cut(s) 244, 805, 1417
Cfr13I GGNCC 7 cut(s) 668, 991, 1220, 1304, 1319, 1344, 1363
CseI GACGC 5 cut(s) 178, 260, 280, 340, 613
Csp6I GTAC 4 cut(s) 694, 700, 1024, 1440
CviAII CATG 6 cut(s) 46, 643, 703, 876, 1039, 1323
CviQI GTAC 4 cut(s) 694, 700, 1024, 1440
DdeI CTNAG 8 cut(s) 118, 233, 717, 1185, 1329, 1479, 1497, 1672
DpnI GATC 6 cut(s) 86, 152, 426, 587, 1253, 1667
DpnII GATC 6 cut(s) 84, 150, 424, 585, 1251, 1665
DrdI GACNNNNNNGTC 1 cut(s) 749
DriI GACNNNNNGTC 1 cut(s) 1196
DseDI GACNNNNNNGTC 1 cut(s) 749
EaeI YGGCCR 4 cut(s) 305, 606, 803, 907
EagI CGGCCG 1 cut(s) 803
Eam1104I CTCTTC 2 cut(s) 923, 1122
Eam1105I GACNNNNNGTC 1 cut(s) 1196
EarI CTCTTC 2 cut(s) 923, 1122
EciI GGCGGA 1 cut(s) 1004
EclXI CGGCCG 1 cut(s) 803
Eco130I CCWWGG 2 cut(s) 466, 1300
Eco147I AGGCCT 2 cut(s) 231, 1275
Eco32I GATATC 2 cut(s) 1165, 1465
Eco47I GGWCC 4 cut(s) 991, 1319, 1344, 1363
Eco52I CGGCCG 1 cut(s) 803
Eco57I CTGAAG 1 cut(s) 1536
Eco72I CACGTG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 1661
EcoNI CCTNNNNNAGG 1 cut(s) 1215
EcoO109I RGGNCCY 2 cut(s) 668, 1363
EcoRI GAATTC 2 cut(s) 1171, 1450
EcoRII CCWGG 5 cut(s) 315, 447, 514, 1222, 1340
EcoRV GATATC 2 cut(s) 1165, 1465
EcoT14I CCWWGG 2 cut(s) 466, 1300
ErhI CCWWGG 2 cut(s) 466, 1300
Esp3I CGTCTC 1 cut(s) 323
FaeI CATG 6 cut(s) 49, 646, 706, 879, 1042, 1326
FalI AAGNNNNNCTT 2 cut(s) 1509, 1541
FatI CATG 6 cut(s) 45, 642, 702, 875, 1038, 1322
FauI CCCGC 3 cut(s) 118, 158, 421
FauNDI CATATG 1 cut(s) 1683
FblI GTMKAC 5 cut(s) 156, 915, 1432, 1533, 1623
FokI GGATG 2 cut(s) 1248, 1520
FspBI CTAG 2 cut(s) 462, 1539
GlaI GCGC 1 cut(s) 173
GsaI CCCAGC 2 cut(s) 281, 1601
GsuI CTGGAG 1 cut(s) 1356
HapII CCGG 5 cut(s) 245, 393, 806, 825, 1418
HgaI GACGC 5 cut(s) 178, 260, 280, 340, 613
HhaI GCGC 1 cut(s) 174
Hin1I GRCGYC 1 cut(s) 624
Hin1II CATG 6 cut(s) 49, 646, 706, 879, 1042, 1326
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HincII GTYRAC 3 cut(s) 157, 685, 1624
HindII GTYRAC 3 cut(s) 157, 685, 1624
HindIII AAGCTT 1 cut(s) 441
HinfI GANTC 7 cut(s) 282, 383, 1081, 1140, 1296, 1475, 1535
HpaI GTTAAC 1 cut(s) 685
HpaII CCGG 5 cut(s) 245, 393, 806, 825, 1418
HphI GGTGA 2 cut(s) 820, 1660
Hpy188I TCNGA 5 cut(s) 366, 374, 865, 1188, 1474
Hpy99I CGWCG 5 cut(s) 158, 161, 221, 425, 542
HpyAV CCTTC 6 cut(s) 104, 221, 950, 1103, 1354, 1499
HpyCH4III ACNGT 4 cut(s) 217, 349, 835, 1549
HpyCH4IV ACGT 4 cut(s) 302, 744, 924, 1382
HpyF10VI GCNNNNNNNGC 8 cut(s) 119, 171, 178, 304, 449, 471, 877, 1049
HpyF3I CTNAG 8 cut(s) 118, 233, 717, 1185, 1329, 1479, 1497, 1672
HpySE526I ACGT 4 cut(s) 302, 744, 924, 1382
Hsp92I GRCGYC 1 cut(s) 624
Hsp92II CATG 6 cut(s) 49, 646, 706, 879, 1042, 1326
HspAI GCGC 1 cut(s) 172
KroI GCCGGC 1 cut(s) 1417
KroNI GCCGGC 1 cut(s) 1419
KspAI GTTAAC 1 cut(s) 685
Kzo9I GATC 6 cut(s) 84, 150, 424, 585, 1251, 1665
LmnI GCTCC 3 cut(s) 471, 713, 1337
Lsp1109I GCAGC 6 cut(s) 181, 253, 540, 1033, 1036, 1433
LweI GCATC 1 cut(s) 214
MaeI CTAG 2 cut(s) 462, 1539
MaeII ACGT 4 cut(s) 302, 744, 924, 1382
MaeIII GTNAC 2 cut(s) 343, 1552
MalI GATC 6 cut(s) 86, 152, 426, 587, 1253, 1667
MbiI CCGCTC 2 cut(s) 165, 885
MboI GATC 6 cut(s) 84, 150, 424, 585, 1251, 1665
MboII GAAGA 4 cut(s) 16, 940, 965, 1139
MfeI CAATTG 1 cut(s) 193
MhlI GDGCHC 3 cut(s) 323, 729, 849
MlsI TGGCCA 1 cut(s) 608
MluCI AATT 5 cut(s) 193, 1171, 1450, 1585, 1636
MluNI TGGCCA 1 cut(s) 608
MlyI GAGTC 2 cut(s) 1075, 1529
MmeI TCCRAC 2 cut(s) 429, 1273
Mox20I TGGCCA 1 cut(s) 608
MroNI GCCGGC 1 cut(s) 1417
MscI TGGCCA 1 cut(s) 608
MseI TTAA 3 cut(s) 506, 684, 1028
MslI CAYNNNNRTG 1 cut(s) 852
Msp20I TGGCCA 1 cut(s) 608
MspCI CTTAAG 1 cut(s) 505
MspI CCGG 5 cut(s) 245, 393, 806, 825, 1418
MspR9I CCNGG 6 cut(s) 317, 449, 516, 826, 1224, 1342
MunI CAATTG 1 cut(s) 193
Mva1269I GAATGC 1 cut(s) 1248
MvaI CCWGG 5 cut(s) 317, 449, 516, 1224, 1342
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 8 cut(s) 119, 171, 178, 304, 449, 471, 877, 1049
NaeI GCCGGC 1 cut(s) 1419
NciI CCSGG 1 cut(s) 826
NdeI CATATG 1 cut(s) 1683
NdeII GATC 6 cut(s) 84, 150, 424, 585, 1251, 1665
NgoMIV GCCGGC 1 cut(s) 1417
NlaIII CATG 6 cut(s) 49, 646, 706, 879, 1042, 1326
NlaIV GGNNCC 6 cut(s) 322, 670, 962, 1003, 1306, 1339
NmeAIII GCCGAG 1 cut(s) 710
NmuCI GTSAC 2 cut(s) 343, 1552
NspI RCATGY 1 cut(s) 706
PaeR7I CTCGAG 1 cut(s) 1661
PceI AGGCCT 2 cut(s) 231, 1275
PcsI WCGNNNNNNNCGW 3 cut(s) 216, 378, 1637
PctI GAATGC 1 cut(s) 1248
PdiI GCCGGC 1 cut(s) 1419
PfeI GAWTC 5 cut(s) 282, 383, 1140, 1296, 1475
PflFI GACNNNGTC 1 cut(s) 215
PflMI CCANNNNNTGG 1 cut(s) 454
PleI GAGTC 2 cut(s) 1075, 1529
PmaCI CACGTG 1 cut(s) 303
PmlI CACGTG 1 cut(s) 303
PpsI GAGTC 2 cut(s) 1075, 1529
Ppu21I YACGTR 1 cut(s) 303
PpuMI RGGWCCY 1 cut(s) 1363
Psp5II RGGWCCY 1 cut(s) 1363
Psp6I CCWGG 5 cut(s) 315, 447, 514, 1222, 1340
PspCI CACGTG 1 cut(s) 303
PspFI CCCAGC 2 cut(s) 277, 1597
PspGI CCWGG 5 cut(s) 315, 447, 514, 1222, 1340
PspN4I GGNNCC 6 cut(s) 322, 670, 962, 1003, 1306, 1339
PspPI GGNCC 7 cut(s) 668, 991, 1220, 1304, 1319, 1344, 1363
PspPPI RGGWCCY 1 cut(s) 1363
PsyI GACNNNGTC 1 cut(s) 215
RsaI GTAC 4 cut(s) 695, 701, 1025, 1441
RsaNI GTAC 4 cut(s) 694, 700, 1024, 1440
RseI CAYNNNNRTG 1 cut(s) 852
SalI GTCGAC 2 cut(s) 155, 1622
SaqAI TTAA 3 cut(s) 506, 684, 1028
Sau3AI GATC 6 cut(s) 84, 150, 424, 585, 1251, 1665
Sau96I GGNCC 7 cut(s) 668, 991, 1220, 1304, 1319, 1344, 1363
SchI GAGTC 2 cut(s) 1075, 1529
ScrFI CCNGG 6 cut(s) 317, 449, 516, 826, 1224, 1342
SduI GDGCHC 3 cut(s) 323, 729, 849
SfaNI GCATC 1 cut(s) 214
Sfr274I CTCGAG 1 cut(s) 1661
SgrDI CGTCGACG 1 cut(s) 155
SinI GGWCC 4 cut(s) 991, 1319, 1344, 1363
SlaI CTCGAG 1 cut(s) 1661
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 5 cut(s) 505, 649, 766, 1573, 1661
SmoI CTYRAG 5 cut(s) 505, 649, 766, 1573, 1661
Sse9I AATT 5 cut(s) 193, 1171, 1450, 1585, 1636
SseBI AGGCCT 2 cut(s) 231, 1275
SsiI CCGC 9 cut(s) 111, 165, 308, 338, 414, 885, 906, 989, 1043
SspMI CTAG 2 cut(s) 462, 1539
StuI AGGCCT 2 cut(s) 231, 1275
StyD4I CCNGG 6 cut(s) 315, 447, 514, 824, 1222, 1340
StyI CCWWGG 2 cut(s) 466, 1300
TaaI ACNGT 4 cut(s) 217, 349, 835, 1549
TaiI ACGT 4 cut(s) 305, 747, 927, 1385
TasI AATT 5 cut(s) 193, 1171, 1450, 1585, 1636
TatI WGTACW 2 cut(s) 699, 1439
TauI GCSGC 5 cut(s) 310, 341, 888, 909, 1046
TfiI GAWTC 5 cut(s) 282, 383, 1140, 1296, 1475
Tru1I TTAA 3 cut(s) 506, 684, 1028
Tru9I TTAA 3 cut(s) 506, 684, 1028
TscAI CASTG 8 cut(s) 352, 459, 595, 729, 854, 1431, 1519, 1613
TseFI GTSAC 2 cut(s) 343, 1552
TseI GCWGC 6 cut(s) 169, 241, 528, 1046, 1049, 1421
Tsp45I GTSAC 2 cut(s) 343, 1552
TspDTI ATGAA 5 cut(s) 17, 672, 1027, 1250, 1341
TspGWI ACGGA 2 cut(s) 794, 1520
TspRI CASTG 8 cut(s) 352, 459, 595, 729, 854, 1431, 1519, 1613
Tth111I GACNNNGTC 1 cut(s) 215
Van91I CCANNNNNTGG 1 cut(s) 454
Vha464I CTTAAG 1 cut(s) 505
VneI GTGCAC 2 cut(s) 725, 845
VpaK11BI GGWCC 4 cut(s) 991, 1319, 1344, 1363
XagI CCTNNNNNAGG 1 cut(s) 1215
XapI RAATTY 3 cut(s) 1171, 1450, 1636
XceI RCATGY 1 cut(s) 706
XcmI CCANNNNNNNNNTGG 2 cut(s) 331, 1605
XhoI CTCGAG 1 cut(s) 1661
XmiI GTMKAC 5 cut(s) 156, 915, 1432, 1533, 1623
XspI CTAG 2 cut(s) 462, 1539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.