pycom13g09080

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010164
Physical Location & Seq
Forward (+)
6073908 .. 6075538
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g09080.2

Sequence Viewer

Length: 1089 bp
ATGGCCACATTGTTCAAAGGCGTCAGTAATGTGGTGGGCATGAGCTTGAGGAATGAGCTGCGAGGCCCCAAACAGAATGTTGACGACTGGTACAAGTACATGCAAAGAGGAGCTGAGGCGGTGCACTCGGCAAACGCAGACGTGCTTGTCATTCTCTCTGGCTTGAGTTTTGACACGGACTTATCTTTCCTCGCCAAACGACCCGTGAGCCTCACATTTGCCGGGAAAACAGTATTTGAAGTGCACTGGTATGGATTTTCAGATGGGCAGGCGTGGAAGAACGGCAATCCCAACCAAGTGTGCGGCCAAGTATACAATAACGTGAAGAGAAAGTCAGGATTTTTGCTTGACAATGGGTTCCCATTGTTTGTGAGTGAGTTTGGGGTGGACCACAGGGGCACCAATGTAAATGACAACAGGTACTTAAACTGCTTCATGGCTGCTGCTGCTGAATTTGATGTGGATTTTGCTCTGTGGACCCTGGTTGGGAGTTATTATTTAAGGCAAGGTGTGGTGGGTATGGAAGAGTATTATGGAATCTTGAACTGGGATTGGAGCGATATCAGGAATTCAAGCCTAACTCAGAGACTCTCTGTCCTCCAATCTCCTTTGCAAGGGCCAGGCTTAGCTCAATCCAGGATGCATAAAATCATTTTCCATCCAGCCACAGGCCTGTGCGTCCTAAAAGTCGGATTCATTGGCCCTTTGAAGTTAGGTCCATGTTCTCAGTCTGGAGCCTGGACCTATTCAACGAGGAAGGTCCTAACGCTGAAAGGAACGTATTTCTGCATACAAGCTGATGGACTCAATAAGCGGGCAGCAGTGGGGATACTTTGCACGACCAGGAATTCGCAATGGGATATCGTTTCGGATTCTAAGTTGCACCTCCAATCTAAGACCATGGATGGTACCGACGTTTGCCTTGACGTAGACTCCAGCAATACCGTTGTCACAAATTCCTGCAACTGCTTGAGCAGAGATAGTTCGTGCGACCCGGGAAGCCAGTGGTTCAAACTTGTCGACAGCACGATAAATTCGTCGCCTAAAAGCCCCATGCTCAAGCTCAACTCAGTCGTGCAAATGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Cellulase Ricin_B_lectin

Protein Analysis

363

Amino Acids

40.02

Weight (kDa)

8.46

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
Acc65I GGTACC 1 cut(s) 908
AccB1I GGYRCC 2 cut(s) 398, 908
AccI GTMKAC 3 cut(s) 312, 930, 1020
AciI CCGC 3 cut(s) 119, 303, 814
AcoI YGGCCR 2 cut(s) 3, 304
AcsI RAATTY 5 cut(s) 452, 568, 847, 955, 1033
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 4 cut(s) 92, 98, 422, 910
AfiI CCNNNNNNNGG 3 cut(s) 486, 614, 668
AgsI TTSAA 7 cut(s) 16, 239, 544, 573, 709, 750, 1012
AhdI GACNNNNNGTC 1 cut(s) 593
AjiI CACGTC 1 cut(s) 142
AjnI CCWGG 5 cut(s) 480, 619, 635, 737, 842
AluBI AGCT 6 cut(s) 45, 58, 113, 629, 797, 1063
AluI AGCT 6 cut(s) 45, 58, 113, 629, 797, 1063
Alw21I GWGCWC 2 cut(s) 126, 246
Alw26I GTCTC 1 cut(s) 580
Alw44I GTGCAC 2 cut(s) 122, 242
Ama87I CYCGRG 1 cut(s) 994
AoxI GGCC 6 cut(s) 3, 64, 304, 617, 670, 700
ApaLI GTGCAC 2 cut(s) 122, 242
ApeKI GCWGC 5 cut(s) 58, 440, 443, 446, 818
ApoI RAATTY 5 cut(s) 452, 568, 847, 955, 1033
ArsI GACNNNNNNTTYG 2 cut(s) 832, 864
Asp718I GGTACC 1 cut(s) 908
AspS9I GGNCC 8 cut(s) 65, 388, 477, 617, 701, 716, 741, 760
AsuC2I CCSGG 3 cut(s) 223, 995, 996
AvaI CYCGRG 1 cut(s) 994
AvaII GGWCC 5 cut(s) 388, 477, 716, 741, 760
BaeGI GKGCMC 3 cut(s) 126, 246, 401
BaeI ACNNNNGTAYC 2 cut(s) 900, 933
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 398, 908
Bbv12I GWGCWC 2 cut(s) 126, 246
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 5 cut(s) 45, 427, 430, 433, 830
BccI CCATC 4 cut(s) 257, 666, 794, 899
BceAI ACGGC 1 cut(s) 298
BciT130I CCWGG 5 cut(s) 482, 621, 637, 739, 844
BciVI GTATCC 1 cut(s) 822
BcnI CCSGG 3 cut(s) 223, 995, 996
BcoDI GTCTC 1 cut(s) 580
BfuI GTATCC 1 cut(s) 822
BisI GCNGC 6 cut(s) 59, 304, 441, 444, 447, 819
BlpI GCTNAGC 1 cut(s) 625
BlsI GCNGC 6 cut(s) 60, 305, 442, 445, 448, 820
Bme1390I CCNGG 8 cut(s) 223, 482, 621, 637, 739, 844, 995, 996
Bme18I GGWCC 5 cut(s) 388, 477, 716, 741, 760
BmeRI GACNNNNNGTC 1 cut(s) 593
BmeT110I CYCGRG 1 cut(s) 994
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 8 cut(s) 65, 388, 477, 617, 701, 716, 741, 760
BmiI GGNNCC 6 cut(s) 67, 359, 400, 479, 736, 910
BmrFI CCNGG 8 cut(s) 223, 482, 621, 637, 739, 844, 995, 996
BmrI ACTGGG 1 cut(s) 556
BmsI GCATC 1 cut(s) 630
BmuI ACTGGG 1 cut(s) 556
BpmI CTGGAG 2 cut(s) 753, 919
Bpu10I CCTNAGC 1 cut(s) 114
Bpu1102I GCTNAGC 1 cut(s) 625
BpuEI CTTGAG 4 cut(s) 67, 184, 991, 1043
BpuMI CCSGG 3 cut(s) 223, 995, 996
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 3 cut(s) 480, 900, 994
BsaXI ACNNNNNCTCC 2 cut(s) 917, 947
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 614, 668
Bse1I ACTGG 4 cut(s) 92, 251, 551, 1003
Bse3DI GCAATG 1 cut(s) 860
BseBI CCWGG 5 cut(s) 482, 621, 637, 739, 844
BseDI CCNNGG 3 cut(s) 480, 900, 994
BseGI GGATG 3 cut(s) 645, 658, 910
BseLI CCNNNNNNNGG 3 cut(s) 486, 614, 668
BseMI GCAATG 1 cut(s) 860
BseMII CTCAG 4 cut(s) 105, 596, 740, 1083
BseNI ACTGG 4 cut(s) 92, 251, 551, 1003
BseRI GAGGAG 1 cut(s) 123
BseSI GKGCMC 3 cut(s) 126, 246, 401
BseXI GCAGC 5 cut(s) 45, 427, 430, 433, 830
BshFI GGCC 6 cut(s) 5, 66, 306, 619, 672, 702
BshNI GGYRCC 2 cut(s) 398, 908
BsiHKAI GWGCWC 2 cut(s) 126, 246
BsiHKCI CYCGRG 1 cut(s) 994
BsiSI CCGG 2 cut(s) 222, 995
BslI CCNNNNNNNGG 3 cut(s) 486, 614, 668
BsmAI GTCTC 1 cut(s) 580
BsnI GGCC 6 cut(s) 5, 66, 306, 619, 672, 702
BsoBI CYCGRG 1 cut(s) 994
Bsp1286I GDGCHC 3 cut(s) 126, 246, 401
Bsp1720I GCTNAGC 1 cut(s) 625
Bsp19I CCATGG 1 cut(s) 900
BspACI CCGC 3 cut(s) 119, 303, 814
BspANI GGCC 6 cut(s) 5, 66, 306, 619, 672, 702
BspCNI CTCAG 4 cut(s) 106, 595, 739, 1082
BspLI GGNNCC 6 cut(s) 67, 359, 400, 479, 736, 910
BspT107I GGYRCC 2 cut(s) 398, 908
BsrDI GCAATG 1 cut(s) 860
BsrI ACTGG 4 cut(s) 92, 251, 551, 1003
BssECI CCNNGG 3 cut(s) 480, 900, 994
BssNAI GTATAC 1 cut(s) 313
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 900
Bst1107I GTATAC 1 cut(s) 313
Bst2UI CCWGG 5 cut(s) 482, 621, 637, 739, 844
Bst4CI ACNGT 2 cut(s) 232, 946
Bst6I CTCTTC 2 cut(s) 320, 519
BstACI GRCGYC 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 270, 816
BstDEI CTNAG 7 cut(s) 114, 582, 625, 726, 876, 894, 1069
BstDSI CCRYGG 1 cut(s) 900
BstENI CCTNNNNNAGG 1 cut(s) 612
BstF5I GGATG 3 cut(s) 645, 658, 910
BstMAI GTCTC 1 cut(s) 580
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstNI CCWGG 5 cut(s) 482, 621, 637, 739, 844
BstNSI RCATGY 1 cut(s) 103
BstSCI CCNGG 8 cut(s) 221, 480, 619, 635, 737, 842, 993, 994
BstSLI GKGCMC 3 cut(s) 126, 246, 401
BstV1I GCAGC 5 cut(s) 45, 427, 430, 433, 830
BstZ17I GTATAC 1 cut(s) 313
BsuI GTATCC 1 cut(s) 822
BsuRI GGCC 6 cut(s) 5, 66, 306, 619, 672, 702
BtgI CCRYGG 1 cut(s) 900
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 3 cut(s) 645, 658, 910
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 3 cut(s) 244, 828, 1010
Cac8I GCNNGC 2 cut(s) 270, 816
Cfr13I GGNCC 8 cut(s) 65, 388, 477, 617, 701, 716, 741, 760
Cfr9I CCCGGG 1 cut(s) 994
CseI GACGC 2 cut(s) 10, 667
Csp6I GTAC 4 cut(s) 91, 97, 421, 909
CviAII CATG 6 cut(s) 40, 100, 436, 720, 901, 1054
CviQI GTAC 4 cut(s) 91, 97, 421, 909
DdeI CTNAG 7 cut(s) 114, 582, 625, 726, 876, 894, 1069
DrdI GACNNNNNNGTC 1 cut(s) 146
DriI GACNNNNNGTC 1 cut(s) 593
DseDI GACNNNNNNGTC 1 cut(s) 146
EaeI YGGCCR 2 cut(s) 3, 304
Eam1104I CTCTTC 2 cut(s) 320, 519
Eam1105I GACNNNNNGTC 1 cut(s) 593
EarI CTCTTC 2 cut(s) 320, 519
Eco130I CCWWGG 1 cut(s) 900
Eco147I AGGCCT 1 cut(s) 672
Eco32I GATATC 2 cut(s) 562, 862
Eco47I GGWCC 5 cut(s) 388, 477, 716, 741, 760
Eco88I CYCGRG 1 cut(s) 994
EcoNI CCTNNNNNAGG 1 cut(s) 612
EcoO109I RGGNCCY 2 cut(s) 65, 760
EcoRI GAATTC 2 cut(s) 568, 847
EcoRII CCWGG 5 cut(s) 480, 619, 635, 737, 842
EcoRV GATATC 2 cut(s) 562, 862
EcoT14I CCWWGG 1 cut(s) 900
EcoT22I ATGCAT 1 cut(s) 645
ErhI CCWWGG 1 cut(s) 900
FaeI CATG 6 cut(s) 43, 103, 439, 723, 904, 1057
FatI CATG 6 cut(s) 39, 99, 435, 719, 900, 1053
FauI CCCGC 1 cut(s) 807
FblI GTMKAC 3 cut(s) 312, 930, 1020
Fnu4HI GCNGC 6 cut(s) 59, 304, 441, 444, 447, 819
FokI GGATG 3 cut(s) 645, 652, 917
Fsp4HI GCNGC 6 cut(s) 59, 304, 441, 444, 447, 819
GluI GCNGC 6 cut(s) 59, 304, 441, 444, 447, 819
GsuI CTGGAG 2 cut(s) 753, 919
HaeIII GGCC 6 cut(s) 5, 66, 306, 619, 672, 702
HapII CCGG 2 cut(s) 222, 995
HgaI GACGC 2 cut(s) 10, 667
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 6 cut(s) 43, 103, 439, 723, 904, 1057
HincII GTYRAC 2 cut(s) 82, 1021
HindII GTYRAC 2 cut(s) 82, 1021
HinfI GANTC 6 cut(s) 537, 588, 693, 804, 872, 932
HpaII CCGG 2 cut(s) 222, 995
Hpy166II GTNNAC 8 cut(s) 82, 124, 244, 313, 388, 477, 931, 1021
Hpy188I TCNGA 4 cut(s) 262, 585, 692, 871
Hpy188III TCNNGA 4 cut(s) 336, 541, 565, 732
Hpy8I GTNNAC 8 cut(s) 82, 124, 244, 313, 388, 477, 931, 1021
Hpy99I CGWCG 2 cut(s) 917, 1042
HpyAV CCTTC 1 cut(s) 751
HpyCH4III ACNGT 2 cut(s) 232, 946
HpyCH4IV ACGT 5 cut(s) 141, 321, 779, 915, 927
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
HpyF3I CTNAG 7 cut(s) 114, 582, 625, 726, 876, 894, 1069
HpySE526I ACGT 5 cut(s) 141, 321, 779, 915, 927
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 6 cut(s) 43, 103, 439, 723, 904, 1057
KpnI GGTACC 1 cut(s) 912
LmnI GCTCC 3 cut(s) 110, 555, 734
Lsp1109I GCAGC 5 cut(s) 45, 427, 430, 433, 830
LweI GCATC 1 cut(s) 630
MaeII ACGT 5 cut(s) 141, 321, 779, 915, 927
MaeIII GTNAC 1 cut(s) 949
MboII GAAGA 3 cut(s) 289, 337, 536
MhlI GDGCHC 3 cut(s) 126, 246, 401
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 452, 568, 847, 955, 1033
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 582, 798, 926
MmeI TCCRAC 1 cut(s) 670
MnlI CCTC 9 cut(s) 42, 56, 101, 109, 200, 221, 608, 747, 896
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 645
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 425, 500
MslI CAYNNNNRTG 1 cut(s) 249
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 222, 995
MspR9I CCNGG 8 cut(s) 223, 482, 621, 637, 739, 844, 995, 996
MvaI CCWGG 5 cut(s) 482, 621, 637, 739, 844
MwoI GCNNNNNNNGC 1 cut(s) 446
NciI CCSGG 3 cut(s) 223, 995, 996
NcoI CCATGG 1 cut(s) 900
NlaIII CATG 6 cut(s) 43, 103, 439, 723, 904, 1057
NlaIV GGNNCC 6 cut(s) 67, 359, 400, 479, 736, 910
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 1 cut(s) 949
NsiI ATGCAT 1 cut(s) 645
NspI RCATGY 1 cut(s) 103
PceI AGGCCT 1 cut(s) 672
PcsI WCGNNNNNNNCGW 1 cut(s) 1034
PfeI GAWTC 3 cut(s) 537, 693, 872
PfoI TCCNGGA 1 cut(s) 635
PkrI GCNGC 6 cut(s) 60, 305, 442, 445, 448, 820
PleI GAGTC 3 cut(s) 582, 798, 926
PpsI GAGTC 3 cut(s) 582, 798, 926
PpuMI RGGWCCY 1 cut(s) 760
Psp5II RGGWCCY 1 cut(s) 760
Psp6I CCWGG 5 cut(s) 480, 619, 635, 737, 842
PspGI CCWGG 5 cut(s) 480, 619, 635, 737, 842
PspN4I GGNNCC 6 cut(s) 67, 359, 400, 479, 736, 910
PspPI GGNCC 8 cut(s) 65, 388, 477, 617, 701, 716, 741, 760
PspPPI RGGWCCY 1 cut(s) 760
RsaI GTAC 4 cut(s) 92, 98, 422, 910
RsaNI GTAC 4 cut(s) 91, 97, 421, 909
RseI CAYNNNNRTG 1 cut(s) 249
SalI GTCGAC 1 cut(s) 1019
SaqAI TTAA 2 cut(s) 425, 500
SatI GCNGC 6 cut(s) 59, 304, 441, 444, 447, 819
Sau96I GGNCC 8 cut(s) 65, 388, 477, 617, 701, 716, 741, 760
SchI GAGTC 3 cut(s) 582, 798, 926
ScrFI CCNGG 8 cut(s) 223, 482, 621, 637, 739, 844, 995, 996
SduI GDGCHC 3 cut(s) 126, 246, 401
SfaNI GCATC 1 cut(s) 630
SinI GGWCC 5 cut(s) 388, 477, 716, 741, 760
SmaI CCCGGG 1 cut(s) 996
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 4 cut(s) 46, 163, 970, 1058
SmoI CTYRAG 4 cut(s) 46, 163, 970, 1058
Sse9I AATT 5 cut(s) 452, 568, 847, 955, 1033
SseBI AGGCCT 1 cut(s) 672
SsiI CCGC 3 cut(s) 119, 303, 814
StuI AGGCCT 1 cut(s) 672
StyD4I CCNGG 8 cut(s) 221, 480, 619, 635, 737, 842, 993, 994
StyI CCWWGG 1 cut(s) 900
TaaI ACNGT 2 cut(s) 232, 946
TaiI ACGT 5 cut(s) 144, 324, 782, 918, 930
TaqI TCGA 1 cut(s) 1020
TasI AATT 5 cut(s) 452, 568, 847, 955, 1033
TatI WGTACW 1 cut(s) 96
TauI GCSGC 1 cut(s) 306
TfiI GAWTC 3 cut(s) 537, 693, 872
Tru1I TTAA 2 cut(s) 425, 500
Tru9I TTAA 2 cut(s) 425, 500
TscAI CASTG 3 cut(s) 251, 828, 1010
TseFI GTSAC 1 cut(s) 949
TseI GCWGC 5 cut(s) 58, 440, 443, 446, 818
Tsp45I GTSAC 1 cut(s) 949
TspDTI ATGAA 2 cut(s) 424, 685
TspGWI ACGGA 1 cut(s) 191
TspMI CCCGGG 1 cut(s) 994
TspRI CASTG 3 cut(s) 251, 828, 1010
VneI GTGCAC 2 cut(s) 122, 242
VpaK11BI GGWCC 5 cut(s) 388, 477, 716, 741, 760
XagI CCTNNNNNAGG 1 cut(s) 612
XapI RAATTY 5 cut(s) 452, 568, 847, 955, 1033
XceI RCATGY 1 cut(s) 103
XmaI CCCGGG 1 cut(s) 994
XmiI GTMKAC 3 cut(s) 312, 930, 1020
Zsp2I ATGCAT 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.