Rorug04G0260800

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010164
Physical Location & Seq
Reverse (-)
43277319 .. 43282851
5533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0260800.1

Sequence Viewer

Length: 3939 bp
ATGGCAGAATCTCTGCTCACTTTTGCTGCCGAGGGAGTACTGACCAAGGTGACTTCGCTTGCTGCTCAAGAATTCAGTCTTGTATTGGGTTTCAAAGAAGATCTGGCACAGCTGCGTGACTCATTCCTCAAGATTCAAGCTATGCTAAGAGATGTCGACCATTCACAAGTTCGAGGGGAGGCTGTGGAAATGTGGGTGAAGGATCTTGAAGACATAGCTAACGAAGCCGATGATGTTTTGGATGAAATTGGGTATGAAGTTCTCCGACGCAAAGTAGAACTCCGAGACCAGATGAAGAAAAAGGTGAAAAATTTCTTGTCCCACTCCAATCCCATTGCCTTTCGCTTACAAATGGCACATAAAATCAAGAAAATCAACACATCTTTGGTGAAATTATTCAATCAGGCAGCTGGCCCTATTGGGTTAGTTGCTAGGACATTAGCAGATGCAACCTCTCAAGATATTGAAGTACTTGATAGGGAGACCGTCTCCAGCTTTGATAGCGATGAGAAGCTCATAATTGGAAGAGAGGAGGTCGCGTCAGAGATGGTCAAAACCTTACTCAAGTCACACTATAATGAAGAAAATTATCTTCCAGTTTTGGCTATCGTTGGAATGCCAGGTCTAGGCAAGACAACTTTAGCTAAATCAATATATAATGAATCTAAGATTGATAGCCACTTCCATGAAAAAATATGGTTGTGTGTATCCACCCCTTTTGATGTCAAGACGATCTTAAGGGGGATCTTGGAAACTTTGAGACCAGAGAAAGCAGCAGTACAAAGTAAGGACGCGATTTGTAAATTCATTCGAGAAGAGGTAAAAGGGAAAAGATACCTTCTCATACTGGATGATGTTTGGAACGAAGATTCTGAAAAATGGAAGGAGTTGAGGAGTTGTTTGTTAACAATTAATGATACTCAAGGAAGCAGCATCATTGTCACTACCCGTAGTGACCGAGTTGCAAAAGTCATGGAAACTCTTCCTAGATGTGATTTAAGAAAACTATCAGATGATGAATGCTGGCTCATAATGAAGGATAAAGCCATTCCAGTTGGGAGTCCTCCTATGTCTAAAGAGCAGGAGACAATTGGTAGAGAGATCGCTAAAAGATGTGGAGGTGTACCTCTAGTGTCAAAGGTTTTGGGAGATGTGATGCGCTCTAAAACAAGTGATGAATGGCGATCAATTTTAAAAAGTAAAACATGGGATTCACCAGAAGGAGAAAAGAGAATTTTTTCAATTTTGAAGTTGAGTTTTGATGAATTGAAATCTCCATCCTTGAAACAATGTTTTGCATATTGCTCGATGTTCTTCAAAGATTTTGATATTGAAAAGGATGACTTGATCCAACTTTGGATGGCTCAGGGATGGCTTCACCCTTGTTCTGACAAAAGCCTAGAGATGGAGGAGAGAGGTAATGAATATTTTAAAATCCTATTGGCGAAGTCTTTTTTTCAAGATGTTACAAAGGATTATGGTGGTAATGTTACCAAATGTAAGATGCACGATCTTGTGCATGATCTTGCAGAACATGTATCAAAATCAAGGAACTTACGCTCACTTTTTTCAAATGGAGAAGTCCTTGGGAGCAACTTACTTAACTTCAAATCTTTACGTGTGTTAAATTTATACAAAGCAGACATTGTGGAGTTGCCAATTTCAATTGGGAAGTTGAAACACTTGAGGTATTTGAATGTTTTGAAAACAAGAATCAAAGCATTTCCCAAATCAATTGGTCAGCTTTATAACTTGCAAACATTGAAAATACCTTATCAGCTTGAAGAATTTCCAGGGGAAATTGCAAATTTGATCAACTTGCGGCACATTTATTTTGGTAGATATATGAAAGTTCCAGGTGGGATATTGAGACGGTTGACTAATCTCCGGTCATTACCTTTTCTCAAGGTGGGTAACAAGACAGGTCCTGGAATTGAGGAATTGGGTTGTTTAAACCAGTTGCACGACACCTTGTCTATATATGGACTGGAAAATGTAGGAGATGGAGAAGAAGCTTCGAAAGCAAACTTGGTCGAGAAGAAACATATACGCAAGTTAATCCTTGATTGGAAGCTTAGTAGGCCAAGCCACAATGTGGAGAATGACGATGATGTACTAGAAGGCCTCCGACCACATTCTAGTTTGGAATTTTTGGAGATTCAGGGATTCATGGGTGTTAAATTTCCATCATGGTTATTCCTAGCCAACCATTTGAAAGAGATTGAATTATCGGGCTGCAACAAATGTGAAGGAGTCCCAGTACTCGGGCATTTACCCAATCTTAGTTGTGTTAAGATTATGAGAATGGAGAACCTAACTCGTATAGGATCTGAGTTTTATGGTGATGACCATGTTAACCGTGGAAATGGATCGAGTAAGGAGGCACGGCCTTTGTTCCCTGCTTTGAAAACATTGCATATTGAGTGGGCCCAGAACCTGATCGAGTGGATGGAAGCGCCAACAGGAGGAGCAACTACAGTGTTTCCATGTCTCGAGGAGCTGACCTTGATTTGCTGTAACAAACTGACAAGTGCTCCCAGTCATTTCCCGTCTCTCAAGAAGTTGGTCATAGAATGGATGGATAGCGGAGGCATGCCAATAGCAAGTATTCTAAGCAATCAACTCACAGCTCTCACTTATCTCAGATTATTGCGTGTGGGGGGACTTGCTTGTCTGCCGGAAGGGATGTTAGAAAACAACAAGAATCTTGCACATTTAGACATAAGTCAGTGTTCAGAGTTGACTTGTATTTCTCCCCAATCACAAGGATTAGAGTACTGCTGCGCATCTCTTTCATATTTGCGGTTATATATTTGTGAGAATCTCAGATATTTACCTGATGGGCTACTCACTCCTTCTCTTAAAGAGATGACGTTGTATGCTTGTGACAATCTAGAGTACATCCCAGATGCTACACACGGTGGTCTCACATCCCTTGAAACATTGTCTTTGACAATGTGCTATAAAATAACTTCCATTCCATTTTCACAAGGCCTGCCATCTCTTGCTCAATTACATATAGACCAGTGTCCGGAATTATCAAGCCTACCGGGTGGACTAGAATACTGTACCTCTATTCGGAGTTTAACAATAGCGATGTGCCCTAAGGTACCCTCCATTTCAATCGAAAGTCTGAGTGCATCCCTCGAAGAGTTGTGTGTAAGTGATCTAGACTCTCTTCCAATTTCACGAGGAGGCTTCACATCACTCCGTGAATTGTCAATCATATATTGTGAAAGTGCTGAAATTGGGCTAGAATTTAGTGCCTGTCTTCAAACCCTCGTCTCTCTTCAAAAATTGACGGTGAAGTTTTGCAAAAATCTGGAGACTATTCCAAGTTCAGACAAGCTCACATCCCTCCGCAGCTTGGAGATTAGAGGGTGTTATAAATTCACATGTCTACCGAATGGGTTAGCAGCATCGTCACAGTCCTGCAGCCTCACCCGTTTGAAGGAATTGACAATTGGTGATTTCTGCGAGGAGCTCGATGAATTCCCAGCTTTTCAGGCTATACCACAACTTGAATCATTAACCATCTGGGGGTGGCCTAAGCTCAAGTCTCTCCCTGAACAAATTCAACACTTGCCTTCTTTAAGGCATTTAGAAATAACATGGTTTCACGGAGTGGAGGCTATTCCAGAGTGGTTGGGAAACCTTGCATCTCTTGAGGACCTGACTATTGAGTGTTTCCCGAGTCTAAAGTATCTACCTTCTGTGGAAGCAATGCAACGCCTCACCAAATTGAAAGAGATAGACATCTCCCGCTGTCCCCTTCTAGAAGAAAGATGCACGGAGGAGAATGGCCCTGAGTGGCCTAAGATTCGTCATCTTCAATTCATCACGAGGTATGGTTGGGATAGTTTTCCATATTACTACAGCATAATTCAAAATGAGACTTGCTTGATTTGCAGGCTTTCTCAAACACCAGATGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Cellulase Ricin_B_lectin

Protein Analysis

1312

Amino Acids

148.06

Weight (kDa)

5.79

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 97 6.7e-21 Rx N-terminal domain
NB-ARC PF00931 184 - 350 7e-34 NB-ARC domain
AAA_22 PF13401 200 - 311 3.8e-06 AAA domain
WHD_DRP PF23559 437 - 510 1.5e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 520 - 836 1.5e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 645 - 769 1.7e-35 R13L1/DRL21 LRRs
LRR_14 PF23598 974 - 1180 2.1e-08 Leucine-rich repeat region
LRR_14 PF23598 1172 - 1264 2e-07 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1190 - 1249 8.5e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1749, 3387
AasI GACNNNNNNGTC 1 cut(s) 2670
Acc16I TGCGCA 1 cut(s) 2785
Acc65I GGTACC 1 cut(s) 3109
AccB1I GGYRCC 1 cut(s) 3109
AccB7I CCANNNNNTGG 1 cut(s) 2095
AccI GTMKAC 2 cut(s) 156, 3400
AccII CGCG 2 cut(s) 539, 794
AccIII TCCGGA 1 cut(s) 3031
AciI CCGC 5 cut(s) 1822, 2586, 2803, 3361, 3763
AclWI GGATC 5 cut(s) 210, 752, 1342, 2335, 2377
AdeI CACNNNGTG 4 cut(s) 2095, 2921, 3212, 3625
AflII CTTAAG 1 cut(s) 736
AflIII ACRYGT 3 cut(s) 1534, 1618, 3395
AhdI GACNNNNNGTC 1 cut(s) 1972
AjnI CCWGG 4 cut(s) 619, 1792, 1855, 1927
AjuI GAANNNNNNNTTGG 4 cut(s) 2012, 2044, 3447, 3479
Alw21I GWGCWC 2 cut(s) 2536, 3486
AlwI GGATC 5 cut(s) 210, 752, 1342, 2335, 2377
AlwNI CAGNNNCTG 2 cut(s) 1928, 2437
Ama87I CYCGRG 3 cut(s) 2264, 2492, 3691
Aor13HI TCCGGA 1 cut(s) 3031
AoxI GGCC 9 cut(s) 412, 2081, 2122, 2387, 2427, 2992, 3545, 3802, 3812
ApaI GGGCCC 1 cut(s) 2431
AseI ATTAAT 1 cut(s) 912
Asp700I GAANNNNTTC 6 cut(s) 311, 395, 981, 1237, 1788, 3573
Asp718I GGTACC 1 cut(s) 3109
AspLEI GCGC 3 cut(s) 1161, 2458, 2786
AspS9I GGNCC 6 cut(s) 413, 1925, 2427, 2428, 3670, 3803
AsuC2I CCSGG 1 cut(s) 3051
AsuII TTCGAA 1 cut(s) 2018
AvaI CYCGRG 3 cut(s) 2264, 2492, 3691
AvaII GGWCC 2 cut(s) 1925, 3670
AxyI CCTNAGG 1 cut(s) 3105
BaeGI GKGCMC 2 cut(s) 2431, 3104
BanI GGYRCC 1 cut(s) 3109
BanII GRGCYC 2 cut(s) 2431, 3486
BauI CACGAG 2 cut(s) 3189, 3841
BbsI GAAGAC 2 cut(s) 216, 3263
Bbv12I GWGCWC 2 cut(s) 2536, 3486
BceAI ACGGC 1 cut(s) 2402
BcgI CGANNNNNNTGC 2 cut(s) 1287, 1321
BciT130I CCWGG 4 cut(s) 621, 1794, 1857, 1929
BciVI GTATCC 1 cut(s) 718
BclI TGATCA 1 cut(s) 1812
BcnI CCSGG 1 cut(s) 3051
BfmI CTRYAG 3 cut(s) 2475, 3433, 3874
BfoI RGCGCY 1 cut(s) 2459
BfrI CTTAAG 1 cut(s) 736
BfuI GTATCC 1 cut(s) 718
BglII AGATCT 1 cut(s) 100
BmcAI AGTACT 4 cut(s) 39, 471, 2262, 2777
Bme1390I CCNGG 5 cut(s) 621, 1794, 1857, 1929, 3051
Bme18I GGWCC 2 cut(s) 1925, 3670
BmeRI GACNNNNNGTC 1 cut(s) 1972
BmeT110I CYCGRG 3 cut(s) 2264, 2492, 3691
BmgT120I GGNCC 6 cut(s) 413, 1925, 2427, 2428, 3670, 3803
BmiI GGNNCC 2 cut(s) 2429, 3111
BmrFI CCNGG 5 cut(s) 621, 1794, 1857, 1929, 3051
BmrI ACTGGG 2 cut(s) 2252, 2532
BmuI ACTGGG 2 cut(s) 2252, 2532
BoxI GACNNNNGTC 2 cut(s) 2724, 3027
BpiI GAAGAC 2 cut(s) 216, 3263
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpmI CTGGAG 2 cut(s) 475, 3344
Bpu10I CCTNAGC 2 cut(s) 1365, 3549
Bpu14I TTCGAA 1 cut(s) 2018
BpuMI CCSGG 1 cut(s) 3051
BsaAI YACGTR 1 cut(s) 1619
BsaBI GATNNNNATC 1 cut(s) 3755
BsaI GGTCTC 4 cut(s) 279, 476, 754, 2930
BsaJI CCNNGG 5 cut(s) 30, 45, 1585, 1793, 2359
BsaWI WCCGGW 2 cut(s) 1887, 3031
BsaXI ACNNNNNCTCC 6 cut(s) 524, 554, 2244, 2274, 2518, 2548
Bse1I ACTGG 8 cut(s) 596, 852, 1052, 1957, 1992, 2258, 2538, 3025
Bse21I CCTNAGG 1 cut(s) 3105
Bse3DI GCAATG 3 cut(s) 333, 2411, 3729
Bse8I GATNNNNATC 1 cut(s) 3755
BseAI TCCGGA 1 cut(s) 3031
BseBI CCWGG 4 cut(s) 621, 1794, 1857, 1929
BseDI CCNNGG 5 cut(s) 30, 45, 1585, 1793, 2359
BseJI GATNNNNATC 1 cut(s) 3755
BseMI GCAATG 3 cut(s) 333, 2411, 3729
BseMII CTCAG 6 cut(s) 1379, 2322, 2656, 2839, 3125, 3798
BseNI ACTGG 8 cut(s) 596, 852, 1052, 1957, 1992, 2258, 2538, 3025
BseRI GAGGAG 8 cut(s) 545, 907, 1424, 2481, 2510, 3207, 3494, 3809
BseSI GKGCMC 2 cut(s) 2431, 3104
BseYI CCCAGC 1 cut(s) 3496
Bsh1236I CGCG 2 cut(s) 539, 794
BshFI GGCC 9 cut(s) 414, 2083, 2124, 2389, 2429, 2994, 3547, 3804, 3814
BshNI GGYRCC 1 cut(s) 3109
BsiHKAI GWGCWC 2 cut(s) 2536, 3486
BsiHKCI CYCGRG 3 cut(s) 2264, 2492, 3691
BsiSI CCGG 4 cut(s) 1888, 2678, 3032, 3050
BslFI GGGAC 4 cut(s) 304, 2240, 2676, 3753
BsmBI CGTCTC 4 cut(s) 493, 1864, 2556, 3289
BsmFI GGGAC 4 cut(s) 304, 2240, 2676, 3753
BsmI GAATGC 2 cut(s) 621, 1025
BsnI GGCC 9 cut(s) 414, 2083, 2124, 2389, 2429, 2994, 3547, 3804, 3814
Bso31I GGTCTC 4 cut(s) 279, 476, 754, 2930
BsoBI CYCGRG 3 cut(s) 2264, 2492, 3691
Bsp119I TTCGAA 1 cut(s) 2018
Bsp120I GGGCCC 1 cut(s) 2427
Bsp1286I GDGCHC 4 cut(s) 2431, 2536, 3104, 3486
Bsp13I TCCGGA 1 cut(s) 3031
BspACI CCGC 5 cut(s) 1822, 2586, 2803, 3361, 3763
BspANI GGCC 9 cut(s) 414, 2083, 2124, 2389, 2429, 2994, 3547, 3804, 3814
BspCNI CTCAG 6 cut(s) 1378, 2323, 2655, 2838, 3126, 3799
BspEI TCCGGA 1 cut(s) 3031
BspFNI CGCG 2 cut(s) 539, 794
BspLI GGNNCC 2 cut(s) 2429, 3111
BspMAI CTGCAG 1 cut(s) 3437
BspPI GGATC 5 cut(s) 210, 752, 1342, 2335, 2377
BspT104I TTCGAA 1 cut(s) 2018
BspT107I GGYRCC 1 cut(s) 3109
BspTI CTTAAG 1 cut(s) 736
BspTNI GGTCTC 4 cut(s) 279, 476, 754, 2930
BsrDI GCAATG 3 cut(s) 333, 2411, 3729
BsrI ACTGG 8 cut(s) 596, 852, 1052, 1957, 1992, 2258, 2538, 3025
BssECI CCNNGG 5 cut(s) 30, 45, 1585, 1793, 2359
BssSI CACGAG 2 cut(s) 3189, 3841
BssT1I CCWWGG 2 cut(s) 45, 1585
Bst2BI CACGAG 2 cut(s) 3189, 3841
Bst2UI CCWGG 4 cut(s) 621, 1794, 1857, 1929
Bst4CI ACNGT 8 cut(s) 487, 1875, 2360, 2479, 2921, 3068, 3304, 3429
Bst6I CTCTTC 6 cut(s) 520, 810, 987, 3144, 3183, 3294
BstAFI CTTAAG 1 cut(s) 736
BstBAI YACGTR 1 cut(s) 1619
BstBI TTCGAA 1 cut(s) 2018
BstC8I GCNNGC 6 cut(s) 60, 412, 1025, 2594, 2996, 3911
BstDSI CCRYGG 1 cut(s) 2359
BstFNI CGCG 2 cut(s) 539, 794
BstH2I RGCGCY 1 cut(s) 2459
BstHHI GCGC 3 cut(s) 1161, 2458, 2786
BstMWI GCNNNNNNNGC 6 cut(s) 224, 501, 2021, 2080, 3506, 3906
BstNI CCWGG 4 cut(s) 621, 1794, 1857, 1929
BstNSI RCATGY 3 cut(s) 1538, 2596, 3399
BstPAI GACNNNNGTC 2 cut(s) 2724, 3027
BstSCI CCNGG 5 cut(s) 619, 1792, 1855, 1927, 3049
BstSFI CTRYAG 3 cut(s) 2475, 3433, 3874
BstSLI GKGCMC 2 cut(s) 2431, 3104
BstUI CGCG 2 cut(s) 539, 794
BstV2I GAAGAC 2 cut(s) 216, 3263
BstX2I RGATCY 4 cut(s) 100, 202, 744, 2327
BstYI RGATCY 4 cut(s) 100, 202, 744, 2327
Bsu36I CCTNAGG 1 cut(s) 3105
BsuI GTATCC 1 cut(s) 718
BsuRI GGCC 9 cut(s) 414, 2083, 2124, 2389, 2429, 2994, 3547, 3804, 3814
BtgI CCRYGG 1 cut(s) 2359
BtgZI GCGATG 2 cut(s) 519, 3110
BtsIMutI CAGTG 3 cut(s) 2484, 2735, 3032
Cac8I GCNNGC 6 cut(s) 60, 412, 1025, 2594, 2996, 3911
CaiI CAGNNNCTG 2 cut(s) 1928, 2437
CfoI GCGC 3 cut(s) 1161, 2458, 2786
Cfr13I GGNCC 6 cut(s) 413, 1925, 2427, 2428, 3670, 3803
CseI GACGC 3 cut(s) 276, 528, 800
CspCI CAANNNNNGTGG 2 cut(s) 700, 735
DraI TTTAAA 3 cut(s) 1194, 1432, 1953
DraIII CACNNNGTG 4 cut(s) 2095, 2921, 3212, 3625
DrdI GACNNNNNNGTC 1 cut(s) 2670
DriI GACNNNNNGTC 1 cut(s) 1972
DseDI GACNNNNNNGTC 1 cut(s) 2670
Eam1104I CTCTTC 6 cut(s) 520, 810, 987, 3144, 3183, 3294
Eam1105I GACNNNNNGTC 1 cut(s) 1972
EarI CTCTTC 6 cut(s) 520, 810, 987, 3144, 3183, 3294
Ecl136II GAGCTC 1 cut(s) 3484
Eco130I CCWWGG 2 cut(s) 45, 1585
Eco147I AGGCCT 2 cut(s) 2124, 2994
Eco24I GRGCYC 2 cut(s) 2431, 3486
Eco31I GGTCTC 4 cut(s) 279, 476, 754, 2930
Eco47I GGWCC 2 cut(s) 1925, 3670
Eco53kI GAGCTC 1 cut(s) 3484
Eco81I CCTNAGG 1 cut(s) 3105
Eco88I CYCGRG 3 cut(s) 2264, 2492, 3691
EcoICRI GAGCTC 1 cut(s) 3484
EcoO109I RGGNCCY 2 cut(s) 1925, 3670
EcoRI GAATTC 2 cut(s) 71, 3491
EcoRII CCWGG 4 cut(s) 619, 1792, 1855, 1927
EcoT14I CCWWGG 2 cut(s) 45, 1585
EcoT38I GRGCYC 2 cut(s) 2431, 3486
ErhI CCWWGG 2 cut(s) 45, 1585
Esp3I CGTCTC 4 cut(s) 493, 1864, 2556, 3289
FalI AAGNNNNNCTT 4 cut(s) 719, 751, 1328, 1360
FaqI GGGAC 4 cut(s) 304, 2240, 2676, 3753
FauI CCCGC 1 cut(s) 3770
FbaI TGATCA 1 cut(s) 1812
FblI GTMKAC 2 cut(s) 156, 3400
FriOI GRGCYC 2 cut(s) 2431, 3486
FspI TGCGCA 1 cut(s) 2785
GlaI GCGC 3 cut(s) 1160, 2457, 2785
GsaI CCCAGC 1 cut(s) 3500
GsuI CTGGAG 2 cut(s) 475, 3344
HaeII RGCGCY 1 cut(s) 2459
HaeIII GGCC 9 cut(s) 414, 2083, 2124, 2389, 2429, 2994, 3547, 3804, 3814
HapII CCGG 4 cut(s) 1888, 2678, 3032, 3050
HgaI GACGC 3 cut(s) 276, 528, 800
HhaI GCGC 3 cut(s) 1161, 2458, 2786
Hin6I GCGC 3 cut(s) 1159, 2456, 2784
HinP1I GCGC 3 cut(s) 1159, 2456, 2784
HincII GTYRAC 5 cut(s) 157, 906, 1878, 2356, 2742
HindII GTYRAC 5 cut(s) 157, 906, 1878, 2356, 2742
HindIII AAGCTT 2 cut(s) 2013, 2072
HpaI GTTAAC 2 cut(s) 906, 2356
HpaII CCGG 4 cut(s) 1888, 2678, 3032, 3050
Hpy166II GTNNAC 8 cut(s) 157, 906, 1124, 1878, 2356, 2742, 3056, 3401
Hpy8I GTNNAC 8 cut(s) 157, 906, 1124, 1878, 2356, 2742, 3056, 3401
Hpy99I CGWCG 1 cut(s) 270
HpyCH4III ACNGT 8 cut(s) 487, 1875, 2360, 2479, 2921, 3068, 3304, 3429
HpyCH4IV ACGT 2 cut(s) 1618, 2873
HpyF10VI GCNNNNNNNGC 6 cut(s) 224, 501, 2021, 2080, 3506, 3906
HpySE526I ACGT 2 cut(s) 1618, 2873
HspAI GCGC 3 cut(s) 1159, 2456, 2784
Kpn2I TCCGGA 1 cut(s) 3031
KpnI GGTACC 1 cut(s) 3113
Ksp22I TGATCA 1 cut(s) 1812
KspAI GTTAAC 2 cut(s) 906, 2356
LmnI GCTCC 5 cut(s) 1590, 2468, 2497, 2539, 3481
MaeII ACGT 2 cut(s) 1618, 2873
MfeI CAATTG 4 cut(s) 1089, 1665, 1734, 3462
MflI RGATCY 4 cut(s) 100, 202, 744, 2327
MhlI GDGCHC 4 cut(s) 2431, 2536, 3104, 3486
MlyI GAGTC 5 cut(s) 113, 1069, 2262, 3167, 3703
MmeI TCCRAC 4 cut(s) 289, 592, 1375, 2152
MroI TCCGGA 1 cut(s) 3031
MroXI GAANNNNTTC 6 cut(s) 311, 395, 981, 1237, 1788, 3573
MslI CAYNNNNRTG 2 cut(s) 576, 684
MspA1I CMGCKG 3 cut(s) 112, 410, 3765
MspCI CTTAAG 1 cut(s) 736
MspI CCGG 4 cut(s) 1888, 2678, 3032, 3050
MspR9I CCNGG 5 cut(s) 621, 1794, 1857, 1929, 3051
MssI GTTTAAAC 1 cut(s) 1953
MunI CAATTG 4 cut(s) 1089, 1665, 1734, 3462
Mva1269I GAATGC 2 cut(s) 621, 1025
MvaI CCWGG 4 cut(s) 621, 1794, 1857, 1929
MvnI CGCG 2 cut(s) 539, 794
MwoI GCNNNNNNNGC 6 cut(s) 224, 501, 2021, 2080, 3506, 3906
NciI CCSGG 1 cut(s) 3051
NlaIV GGNNCC 2 cut(s) 2429, 3111
NmeAIII GCCGAG 1 cut(s) 55
NmuCI GTSAC 7 cut(s) 49, 116, 567, 940, 953, 2885, 3423
NsbI TGCGCA 1 cut(s) 2785
NspI RCATGY 3 cut(s) 1538, 2596, 3399
NspV TTCGAA 1 cut(s) 2018
PaeI GCATGC 1 cut(s) 2596
PaeR7I CTCGAG 1 cut(s) 2492
PceI AGGCCT 2 cut(s) 2124, 2994
PciI ACATGT 2 cut(s) 1534, 3395
PctI GAATGC 2 cut(s) 621, 1025
PdmI GAANNNNTTC 6 cut(s) 311, 395, 981, 1237, 1788, 3573
PflMI CCANNNNNTGG 1 cut(s) 2095
PfoI TCCNGGA 1 cut(s) 1927
PleI GAGTC 5 cut(s) 113, 1068, 2261, 3167, 3702
PmeI GTTTAAAC 1 cut(s) 1953
PpsI GAGTC 5 cut(s) 113, 1068, 2261, 3167, 3702
Ppu21I YACGTR 1 cut(s) 1619
PpuMI RGGWCCY 2 cut(s) 1925, 3670
PscI ACATGT 2 cut(s) 1534, 3395
PshAI GACNNNNGTC 2 cut(s) 2724, 3027
PshBI ATTAAT 1 cut(s) 912
PsiI TTATAA 2 cut(s) 1749, 3387
Psp124BI GAGCTC 1 cut(s) 3486
Psp5II RGGWCCY 2 cut(s) 1925, 3670
Psp6I CCWGG 4 cut(s) 619, 1792, 1855, 1927
PspFI CCCAGC 1 cut(s) 3496
PspGI CCWGG 4 cut(s) 619, 1792, 1855, 1927
PspN4I GGNNCC 2 cut(s) 2429, 3111
PspOMI GGGCCC 1 cut(s) 2427
PspPI GGNCC 6 cut(s) 413, 1925, 2427, 2428, 3670, 3803
PspPPI RGGWCCY 2 cut(s) 1925, 3670
PstI CTGCAG 1 cut(s) 3437
PstNI CAGNNNCTG 2 cut(s) 1928, 2437
PsuI RGATCY 4 cut(s) 100, 202, 744, 2327
PvuII CAGCTG 2 cut(s) 112, 410
RseI CAYNNNNRTG 2 cut(s) 576, 684
SacI GAGCTC 1 cut(s) 3486
SalI GTCGAC 1 cut(s) 155
Sau96I GGNCC 6 cut(s) 413, 1925, 2427, 2428, 3670, 3803
ScaI AGTACT 4 cut(s) 39, 471, 2262, 2777
SchI GAGTC 5 cut(s) 113, 1069, 2262, 3167, 3703
ScrFI CCNGG 5 cut(s) 621, 1794, 1857, 1929, 3051
SduI GDGCHC 4 cut(s) 2431, 2536, 3104, 3486
SfcI CTRYAG 3 cut(s) 2475, 3433, 3874
Sfr274I CTCGAG 1 cut(s) 2492
SfuI TTCGAA 1 cut(s) 2018
SinI GGWCC 2 cut(s) 1925, 3670
SlaI CTCGAG 1 cut(s) 2492
SmiMI CAYNNNNRTG 2 cut(s) 576, 684
SphI GCATGC 1 cut(s) 2596
SseBI AGGCCT 2 cut(s) 2124, 2994
SsiI CCGC 5 cut(s) 1822, 2586, 2803, 3361, 3763
SspI AATATT 1 cut(s) 1427
SstI GAGCTC 1 cut(s) 3486
StuI AGGCCT 2 cut(s) 2124, 2994
StyD4I CCNGG 5 cut(s) 619, 1792, 1855, 1927, 3049
StyI CCWWGG 2 cut(s) 45, 1585
TaaI ACNGT 8 cut(s) 487, 1875, 2360, 2479, 2921, 3068, 3304, 3429
TaiI ACGT 2 cut(s) 1621, 2876
TaqII GACCGA 1 cut(s) 972
TatI WGTACW 7 cut(s) 37, 469, 778, 2113, 2260, 2775, 2898
TauI GCSGC 1 cut(s) 1825
TscAI CASTG 3 cut(s) 2484, 2735, 3032
TseFI GTSAC 7 cut(s) 49, 116, 567, 940, 953, 2885, 3423
Tsp45I GTSAC 7 cut(s) 49, 116, 567, 940, 953, 2885, 3423
TspGWI ACGGA 3 cut(s) 3200, 3636, 3806
TspRI CASTG 3 cut(s) 2484, 2735, 3032
Van91I CCANNNNNTGG 1 cut(s) 2095
Vha464I CTTAAG 1 cut(s) 736
VpaK11BI GGWCC 2 cut(s) 1925, 3670
VspI ATTAAT 1 cut(s) 912
XbaI TCTAGA 3 cut(s) 2893, 3169, 3775
XceI RCATGY 3 cut(s) 1538, 2596, 3399
XcmI CCANNNNNNNNNTGG 1 cut(s) 2357
XhoI CTCGAG 1 cut(s) 2492
XmiI GTMKAC 2 cut(s) 156, 3400
XmnI GAANNNNTTC 6 cut(s) 311, 395, 981, 1237, 1788, 3573
ZrmI AGTACT 4 cut(s) 39, 471, 2262, 2777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.