Rh4DG319000

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010164
Physical Location & Seq
Reverse (-)
54220786 .. 54226119
5334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG319000.1

Sequence Viewer

Length: 1182 bp
ATGGGCACGATGACTCTCCTCTCCCTCCTCTCACTTGCCTCTCTTCTAACCGCCACCGTTGCTCTGCCGCTCTCCACCTCCTCGCGGTGGATCGTCGACGAGAACGGGCAGCGCGTGAAGCTGGCGTGCGTGAACTGGGTGGCGCATCTCGACGCCGTCGTCGCTGAAGGCCTCAGCAAGCAGCCCCTCGACGCCATCTCCAAGCGTGTCACCTCCCTCGGCTTCAACTGCGTCAGGCTGACGTGGCCGCTTTACTTGGCCACCAACGACTCCCTCGCCGCCGTCACTGTTCGTCAGTCGTTTCAGAGTCTCGGGCTGCAGGAATCCATTGCTGGGGTTCAGGCCAACAACCCCGCCCTCATCGATCTTCCGCTGTTAAAAGCTTTCCAGGAAGTGGTTTCTAGCCTTGGGAACAACAATGTGATGGTTATACTGGATAATCACATAAGCAAGCCTGGTTGGTGTTGCAGCAGCAATGATGACAATGGTTTCTTTGGGGACAAGTACTTTAACCCTGATCTTTGGATCAAGGGCCTTACTCAGATGGCCACGTTGTTCAAAGGCGTGTCCAATGTGGTGGGCATGAGCTTGAGGAATGAGCTGCGAGGACCCAAACAGAATGTCGAAGATTGGTACAAGTACATGCCGAGAGGAGCTGAGGCAGTGCACTCAGCAAACCCAGATGTGCTTGTGATTTTATCTGGCCTGAGTTATGACACAGACTTATCTTTCCTTAGCAACCAACCAGTGAAACTTACATTTTCCGGGAAACTAGTATATGAGTTGCACTGGTATGGTTTTTCAGATGGGCAGGCATGGAGGGATGGCAACCCCAACCAAGTCTGTGGGCGCGTGGCGGGTGAAGTGAAGAGAAAGGCAGGGTTTTTGCTAGATAAAGGGTTCCCATTATTTGTGAGTGAGTTTGGTGTTGACCAAAGAGGAACCAATGTGAATGACAATAGGTACTTAAACTGCTTCCTAGCTGCAGCTGCTGAACTTGATGTGGAGTTTGCTTTTTGGACTCTGGTTGGAAGTTATTATTTCAGGAGTGGGGTGGTCGGCATGAATGAGTATTATGGGCTGTTGAACTGGAATTGGGGTGAAATCCGTAATTCAAGTTTGCTGCAGCGACTCTCTGTCCTCCAATCTCCTCTCCAAGGTAGTGTTCCAGTTCTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Cellulase Ricin_B_lectin

Protein Analysis

393

Amino Acids

43.31

Weight (kDa)

5.87

Isoelectric Point (pI)

30.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cellulase PF00150 62 - 342 1.2e-25 Cellulase (glycosyl hydrolase family 5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AccB7I CCANNNNNTGG 1 cut(s) 394
AccBSI CCGCTC 1 cut(s) 70
AccI GTMKAC 1 cut(s) 96
AccII CGCG 3 cut(s) 85, 114, 852
AciI CCGC 8 cut(s) 51, 68, 85, 248, 279, 354, 371, 857
AclWI GGATC 2 cut(s) 98, 533
AcoI YGGCCR 3 cut(s) 245, 258, 546
AcuI CTGAAG 1 cut(s) 186
AcyI GRCGYC 2 cut(s) 153, 192
AfaI GTAC 4 cut(s) 506, 635, 641, 965
AfiI CCNNNNNNNGG 5 cut(s) 84, 87, 333, 394, 1157
AgsI TTSAA 4 cut(s) 226, 559, 1087, 1116
AhdI GACNNNNNGTC 1 cut(s) 1136
AhlI ACTAGT 1 cut(s) 772
AjiI CACGTC 1 cut(s) 243
AjnI CCWGG 2 cut(s) 387, 454
AjuI GAANNNNNNNTTGG 2 cut(s) 1149, 1181
AluBI AGCT 7 cut(s) 121, 383, 588, 601, 656, 983, 989
AluI AGCT 7 cut(s) 121, 383, 588, 601, 656, 983, 989
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 314
Alw44I GTGCAC 1 cut(s) 665
AlwI GGATC 2 cut(s) 98, 533
AlwNI CAGNNNCTG 1 cut(s) 992
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 7 cut(s) 169, 245, 258, 342, 532, 546, 703
ApaLI GTGCAC 1 cut(s) 665
ArsI GACNNNNNNTTYG 2 cut(s) 606, 638
AspLEI GCGC 3 cut(s) 114, 145, 852
AspS9I GGNCC 2 cut(s) 532, 608
AsuC2I CCSGG 1 cut(s) 766
AsuHPI GGTGA 3 cut(s) 202, 872, 1112
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 1 cut(s) 608
BaeGI GKGCMC 2 cut(s) 8, 669
BalI TGGCCA 2 cut(s) 260, 548
Bbv12I GWGCWC 1 cut(s) 669
BbvCI CCTCAGC 2 cut(s) 173, 657
BccI CCATC 5 cut(s) 203, 418, 538, 800, 818
BceAI ACGGC 2 cut(s) 140, 266
BciT130I CCWGG 2 cut(s) 389, 456
BcnI CCSGG 1 cut(s) 766
BcoDI GTCTC 1 cut(s) 314
BcuI ACTAGT 1 cut(s) 772
BfaI CTAG 4 cut(s) 402, 773, 890, 980
BfmI CTRYAG 3 cut(s) 317, 984, 1124
BmcAI AGTACT 1 cut(s) 506
Bme1390I CCNGG 3 cut(s) 389, 456, 766
Bme18I GGWCC 1 cut(s) 608
BmeRI GACNNNNNGTC 1 cut(s) 1136
BmeT110I CYCGRG 1 cut(s) 311
BmgBI CACGTC 1 cut(s) 243
BmgT120I GGNCC 2 cut(s) 532, 608
BmiI GGNNCC 3 cut(s) 610, 902, 943
BmrFI CCNGG 3 cut(s) 389, 456, 766
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 1 cut(s) 154
BmuI ACTGGG 1 cut(s) 145
Bpu10I CCTNAGC 3 cut(s) 173, 657, 734
BpuEI CTTGAG 1 cut(s) 610
BpuMI CCSGG 1 cut(s) 766
Bsa29I ATCGAT 1 cut(s) 363
BsaHI GRCGYC 2 cut(s) 153, 192
BsaJI CCNNGG 3 cut(s) 217, 406, 1156
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 5 cut(s) 84, 87, 333, 394, 1157
Bse1I ACTGG 6 cut(s) 140, 438, 746, 794, 1094, 1169
Bse3DI GCAATG 2 cut(s) 327, 481
BseBI CCWGG 2 cut(s) 389, 456
BseCI ATCGAT 1 cut(s) 363
BseDI CCNNGG 3 cut(s) 217, 406, 1156
BseGI GGATG 1 cut(s) 829
BseLI CCNNNNNNNGG 5 cut(s) 84, 87, 333, 394, 1157
BseMI GCAATG 2 cut(s) 327, 481
BseMII CTCAG 5 cut(s) 187, 554, 648, 684, 698
BseNI ACTGG 6 cut(s) 140, 438, 746, 794, 1094, 1169
BseRI GAGGAG 5 cut(s) 8, 17, 70, 666, 1140
BseSI GKGCMC 2 cut(s) 8, 669
BseYI CCCAGC 1 cut(s) 332
Bsh1236I CGCG 3 cut(s) 85, 114, 852
BshFI GGCC 7 cut(s) 171, 247, 260, 344, 534, 548, 705
BshVI ATCGAT 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 1 cut(s) 765
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 5 cut(s) 84, 87, 333, 394, 1157
BsmAI GTCTC 1 cut(s) 314
BsmFI GGGAC 1 cut(s) 512
BsnI GGCC 7 cut(s) 171, 247, 260, 344, 534, 548, 705
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 8, 669
Bsp143I GATC 4 cut(s) 90, 364, 517, 525
BspACI CCGC 8 cut(s) 51, 68, 85, 248, 279, 354, 371, 857
BspANI GGCC 7 cut(s) 171, 247, 260, 344, 534, 548, 705
BspCNI CTCAG 5 cut(s) 186, 553, 649, 683, 699
BspDI ATCGAT 1 cut(s) 363
BspFNI CGCG 3 cut(s) 85, 114, 852
BspLI GGNNCC 3 cut(s) 610, 902, 943
BspMAI CTGCAG 3 cut(s) 321, 988, 1128
BspPI GGATC 2 cut(s) 98, 533
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 2 cut(s) 327, 481
BsrI ACTGG 6 cut(s) 140, 438, 746, 794, 1094, 1169
BssECI CCNNGG 3 cut(s) 217, 406, 1156
BssMI GATC 4 cut(s) 90, 364, 517, 525
BssNI GRCGYC 2 cut(s) 153, 192
BssT1I CCWWGG 2 cut(s) 406, 1156
Bst2UI CCWGG 2 cut(s) 389, 456
Bst4CI ACNGT 2 cut(s) 58, 289
Bst6I CTCTTC 2 cut(s) 48, 863
BstACI GRCGYC 2 cut(s) 153, 192
BstC8I GCNNGC 5 cut(s) 123, 127, 179, 452, 813
BstDEI CTNAG 6 cut(s) 173, 540, 657, 670, 707, 734
BstENI CCTNNNNNAGG 1 cut(s) 1155
BstF5I GGATG 1 cut(s) 829
BstFNI CGCG 3 cut(s) 85, 114, 852
BstHHI GCGC 3 cut(s) 114, 145, 852
BstKTI GATC 4 cut(s) 93, 367, 520, 528
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 4 cut(s) 90, 364, 517, 525
BstMWI GCNNNNNNNGC 6 cut(s) 59, 118, 161, 228, 244, 989
BstNI CCWGG 2 cut(s) 389, 456
BstNSI RCATGY 1 cut(s) 646
BstSCI CCNGG 3 cut(s) 387, 454, 764
BstSFI CTRYAG 3 cut(s) 317, 984, 1124
BstSLI GKGCMC 2 cut(s) 8, 669
BstUI CGCG 3 cut(s) 85, 114, 852
BstXI CCANNNNNNTGG 2 cut(s) 577, 845
Bsu15I ATCGAT 1 cut(s) 363
BsuRI GGCC 7 cut(s) 171, 247, 260, 344, 534, 548, 705
BsuTUI ATCGAT 1 cut(s) 363
BtrI CACGTC 1 cut(s) 243
BtsCI GGATG 1 cut(s) 829
BtsI GCAGTG 1 cut(s) 669
BtsIMutI CAGTG 4 cut(s) 285, 669, 753, 787
Cac8I GCNNGC 5 cut(s) 123, 127, 179, 452, 813
CaiI CAGNNNCTG 1 cut(s) 992
CfoI GCGC 3 cut(s) 114, 145, 852
Cfr13I GGNCC 2 cut(s) 532, 608
ClaI ATCGAT 1 cut(s) 363
CseI GACGC 3 cut(s) 161, 200, 220
Csp6I GTAC 4 cut(s) 505, 634, 640, 964
CviAII CATG 4 cut(s) 583, 643, 816, 1063
CviQI GTAC 4 cut(s) 505, 634, 640, 964
DdeI CTNAG 6 cut(s) 173, 540, 657, 670, 707, 734
DpnI GATC 4 cut(s) 92, 366, 519, 527
DpnII GATC 4 cut(s) 90, 364, 517, 525
DrdI GACNNNNNNGTC 1 cut(s) 158
DriI GACNNNNNGTC 1 cut(s) 1136
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 3 cut(s) 245, 258, 546
Eam1104I CTCTTC 2 cut(s) 48, 863
Eam1105I GACNNNNNGTC 1 cut(s) 1136
EarI CTCTTC 2 cut(s) 48, 863
Eco130I CCWWGG 2 cut(s) 406, 1156
Eco147I AGGCCT 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 608
Eco57I CTGAAG 1 cut(s) 186
Eco88I CYCGRG 1 cut(s) 311
EcoNI CCTNNNNNAGG 1 cut(s) 1155
EcoO109I RGGNCCY 2 cut(s) 532, 608
EcoRII CCWGG 2 cut(s) 387, 454
EcoT14I CCWWGG 2 cut(s) 406, 1156
ErhI CCWWGG 2 cut(s) 406, 1156
FaeI CATG 4 cut(s) 586, 646, 819, 1066
FaqI GGGAC 1 cut(s) 512
FatI CATG 4 cut(s) 582, 642, 815, 1062
FauI CCCGC 2 cut(s) 361, 850
FblI GTMKAC 1 cut(s) 96
FokI GGATG 1 cut(s) 836
FspBI CTAG 4 cut(s) 402, 773, 890, 980
GlaI GCGC 3 cut(s) 113, 144, 851
GsaI CCCAGC 1 cut(s) 336
HaeIII GGCC 7 cut(s) 171, 247, 260, 344, 534, 548, 705
HapII CCGG 1 cut(s) 765
HgaI GACGC 3 cut(s) 161, 200, 220
HhaI GCGC 3 cut(s) 114, 145, 852
Hin1I GRCGYC 2 cut(s) 153, 192
Hin1II CATG 4 cut(s) 586, 646, 819, 1066
Hin6I GCGC 3 cut(s) 112, 143, 850
HinP1I GCGC 3 cut(s) 112, 143, 850
HincII GTYRAC 2 cut(s) 97, 931
HindII GTYRAC 2 cut(s) 97, 931
HindIII AAGCTT 1 cut(s) 381
HinfI GANTC 6 cut(s) 13, 269, 307, 323, 1021, 1131
HpaII CCGG 1 cut(s) 765
HphI GGTGA 3 cut(s) 202, 872, 1112
Hpy166II GTNNAC 4 cut(s) 97, 133, 667, 931
Hpy188I TCNGA 3 cut(s) 306, 543, 805
Hpy188III TCNNGA 2 cut(s) 149, 1045
Hpy8I GTNNAC 4 cut(s) 97, 133, 667, 931
Hpy99I CGWCG 6 cut(s) 98, 101, 155, 161, 164, 194
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 2 cut(s) 58, 289
HpyCH4IV ACGT 2 cut(s) 242, 551
HpyCH4V TGCA 6 cut(s) 319, 468, 667, 787, 986, 1126
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 118, 161, 228, 244, 989
HpyF3I CTNAG 6 cut(s) 173, 540, 657, 670, 707, 734
HpySE526I ACGT 2 cut(s) 242, 551
Hsp92I GRCGYC 2 cut(s) 153, 192
Hsp92II CATG 4 cut(s) 586, 646, 819, 1066
HspAI GCGC 3 cut(s) 112, 143, 850
Kzo9I GATC 4 cut(s) 90, 364, 517, 525
LmnI GCTCC 1 cut(s) 653
LweI GCATC 1 cut(s) 154
MaeI CTAG 4 cut(s) 402, 773, 890, 980
MaeII ACGT 2 cut(s) 242, 551
MaeIII GTNAC 2 cut(s) 208, 283
MalI GATC 4 cut(s) 92, 366, 519, 527
MbiI CCGCTC 1 cut(s) 70
MboI GATC 4 cut(s) 90, 364, 517, 525
MboII GAAGA 5 cut(s) 35, 359, 638, 880, 1166
MhlI GDGCHC 2 cut(s) 8, 669
MlsI TGGCCA 2 cut(s) 260, 548
MluCI AATT 2 cut(s) 1093, 1111
MluNI TGGCCA 2 cut(s) 260, 548
MlyI GAGTC 5 cut(s) 7, 263, 316, 1015, 1125
MmeI TCCRAC 1 cut(s) 1009
Mox20I TGGCCA 2 cut(s) 260, 548
MscI TGGCCA 2 cut(s) 260, 548
MseI TTAA 3 cut(s) 377, 510, 968
MslI CAYNNNNRTG 1 cut(s) 792
Msp20I TGGCCA 2 cut(s) 260, 548
MspA1I CMGCKG 2 cut(s) 373, 989
MspI CCGG 1 cut(s) 765
MspR9I CCNGG 3 cut(s) 389, 456, 766
MvaI CCWGG 2 cut(s) 389, 456
MvnI CGCG 3 cut(s) 85, 114, 852
MwoI GCNNNNNNNGC 6 cut(s) 59, 118, 161, 228, 244, 989
NciI CCSGG 1 cut(s) 766
NdeII GATC 4 cut(s) 90, 364, 517, 525
NlaIII CATG 4 cut(s) 586, 646, 819, 1066
NlaIV GGNNCC 3 cut(s) 610, 902, 943
NmeAIII GCCGAG 2 cut(s) 198, 672
NmuCI GTSAC 2 cut(s) 208, 283
NspI RCATGY 1 cut(s) 646
PceI AGGCCT 1 cut(s) 171
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 156
PfeI GAWTC 1 cut(s) 323
PflFI GACNNNGTC 1 cut(s) 155
PflMI CCANNNNNTGG 1 cut(s) 394
PfoI TCCNGGA 2 cut(s) 387, 764
PleI GAGTC 5 cut(s) 7, 263, 315, 1015, 1125
PpsI GAGTC 5 cut(s) 7, 263, 315, 1015, 1125
PpuMI RGGWCCY 1 cut(s) 608
Psp5II RGGWCCY 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 387, 454
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 2 cut(s) 387, 454
PspN4I GGNNCC 3 cut(s) 610, 902, 943
PspPI GGNCC 2 cut(s) 532, 608
PspPPI RGGWCCY 1 cut(s) 608
PstI CTGCAG 3 cut(s) 321, 988, 1128
PstNI CAGNNNCTG 1 cut(s) 992
PsyI GACNNNGTC 1 cut(s) 155
PvuII CAGCTG 1 cut(s) 989
RsaI GTAC 4 cut(s) 506, 635, 641, 965
RsaNI GTAC 4 cut(s) 505, 634, 640, 964
RseI CAYNNNNRTG 1 cut(s) 792
SalI GTCGAC 1 cut(s) 95
SaqAI TTAA 3 cut(s) 377, 510, 968
Sau3AI GATC 4 cut(s) 90, 364, 517, 525
Sau96I GGNCC 2 cut(s) 532, 608
ScaI AGTACT 1 cut(s) 506
SchI GAGTC 5 cut(s) 7, 263, 316, 1015, 1125
ScrFI CCNGG 3 cut(s) 389, 456, 766
SduI GDGCHC 2 cut(s) 8, 669
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 3 cut(s) 317, 984, 1124
SgrDI CGTCGACG 1 cut(s) 95
SinI GGWCC 1 cut(s) 608
SmiMI CAYNNNNRTG 1 cut(s) 792
SmlI CTYRAG 1 cut(s) 589
SmoI CTYRAG 1 cut(s) 589
SpeI ACTAGT 1 cut(s) 772
Sse9I AATT 2 cut(s) 1093, 1111
SseBI AGGCCT 1 cut(s) 171
SsiI CCGC 8 cut(s) 51, 68, 85, 248, 279, 354, 371, 857
SspMI CTAG 4 cut(s) 402, 773, 890, 980
StuI AGGCCT 1 cut(s) 171
StyD4I CCNGG 3 cut(s) 387, 454, 764
StyI CCWWGG 2 cut(s) 406, 1156
TaaI ACNGT 2 cut(s) 58, 289
TaiI ACGT 2 cut(s) 245, 554
TaqI TCGA 5 cut(s) 96, 150, 189, 363, 624
TasI AATT 2 cut(s) 1093, 1111
TatI WGTACW 2 cut(s) 504, 639
TauI GCSGC 3 cut(s) 70, 250, 281
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 3 cut(s) 377, 510, 968
Tru9I TTAA 3 cut(s) 377, 510, 968
TscAI CASTG 4 cut(s) 292, 669, 753, 794
TseFI GTSAC 2 cut(s) 208, 283
Tsp45I GTSAC 2 cut(s) 208, 283
TspDTI ATGAA 1 cut(s) 1079
TspGWI ACGGA 1 cut(s) 1097
TspRI CASTG 4 cut(s) 292, 669, 753, 794
Tth111I GACNNNGTC 1 cut(s) 155
Van91I CCANNNNNTGG 1 cut(s) 394
VneI GTGCAC 1 cut(s) 665
VpaK11BI GGWCC 1 cut(s) 608
XagI CCTNNNNNAGG 1 cut(s) 1155
XceI RCATGY 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 96
XspI CTAG 4 cut(s) 402, 773, 890, 980
ZrmI AGTACT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.