pycom16g08910

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010164
Physical Location & Seq
Forward (+)
5953114 .. 5957348
4235 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g08910.1

Sequence Viewer

Length: 1701 bp
ATGAAGGTTGAGTTTCCATATACTCTCAACATTCCTCCCAACATGGCAAGGTCAATCATCTCATTTTTCTCCTTTATCTCGATCCTTTCCCTTCTCACACTCACGCCCGCAGTCTCAGCTCTCCCGCTCTCCACCTCCTCCCGATGGATCGTCGACGAGAGTGGGCAGCGCGTGAAGCTGGCCTGCGTGAATTGGGTGTCGCATCTCGACGCCGTCGTAGCCGAAGGCCTCAGCAAGCAGCCGGTGGACGCAATCGCCAAGCGCATCACGTCGCTGGGATTCAACTGCGTCAGGCTCACGTGGCCGCTTTTCCTGGCCACCAATGAGACGCTGGCGGCTGTCACTGTTCGGCAGTCGTTTCAGAGCCTCGGACTGCTCGATTCTATTTCCGGTATCCAGTCCAACAATCCCGCCCTCGTCGATCTCACCCTCTTGAAAGCTTACCAGGCAGTGGTTTCTAGCCTTGGGAGCAACAATGTGATGGTTATACTGGACAATCACTTAAGCAAGCCTGGGTGGTGTTGCAGCAACACCGACGGCAATGGTTTCTTTGGCGACCAGTATTTCAACCCTGACCTTTGGATCGATGGCCTAACTCGAATGGCCACATTGTTCAAAGGCGTCAGCAATGTGGTCGGCATGAGCTTGAGGAATGAGCTGCGAGGCCCCAAACAGAATGTTAACGATTGGTACAAGTACATGCAAAGAGGAGCTGAGGCAGTGCACTTGGCAAACGCAGACGTGCTGGTCATTCTCTCTGGCTTGAGTTTTGACACGGACTTATCTTTCCTAGCCAAACGGCCTGTGAACCTCACATTTGCGGGGAAAACAGTATTTGAAGTGCACTGGTATGGATTTTCAGACGGGCAGGCATGGCAGAGCGGCAATCCCAACCAAGTGTGCGGCCAAGTATACAATAACGTGAAGAGAAAGTCAGGATTTTTGCTTGAAGAAGGGTTCCCATTGTTTGTGAGTGAGTTTGGAGCGGACCAAAGGGGAACCAATGTAAATGACAACAGGTACTTAAACTGCTTCATGGCTGCTGCTGCTGAACTTGATGTGGATTTTGCTTTGTGGACTCTGGTTGGGAGTTATTACTTTAGGGAAGGGGTGGTGGGTGCGGAAGAGTATTATGGAATCTTGAACTGGGATTGGAGTGATATCAGGAATTCAAGCCTAACTCAGAGACTTTCTGTCCTCCAATCGCCTTTGCAAGGGCCAGGCTTATCTCAATCCAGAATGCACAAGATCATATTTCATCCAGCCACAGGCCTTTGCCTCCTAAAAGTTGGATTCCTTGGCCCTTTGAAGTTAGGTCCATGCTCTCAGTCTGGAGCTTGGACCTATTCATCCAGGAAGGTCCTAACGCTGAAAGGAACGTATTTCTGCATACAAGCTGACGAACTGAACAAGCCGGCAGCAGTGGGGATACTTTGCACGACCACGAATTCACAATGGGATATCATTTCGGATTCTAAGTTGCACCTTCAATCTAAGACCACGGACGGCACTGAAGTTTGCCTTGATGTAGACTCCAGCAATACAGTTGTCACAAGTTCCTGCAAATGCTTGAGCAGAGATAATTCTTGCGACCCAGCTAGCCAGTGGTTCAAACTTGTCGACAGCACGATAAATACGACACCTACAGGCCCCATTCTCGAGATAACCTCAGTCGTGCATATGATTGAGGCAACTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Cellulase

Protein Analysis

567

Amino Acids

62.13

Weight (kDa)

5.95

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cellulase PF00150 81 - 361 1e-22 Cellulase (glycosyl hydrolase family 5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 746
AccB7I CCANNNNNTGG 1 cut(s) 451
AccBSI CCGCTC 3 cut(s) 127, 882, 986
AccI GTMKAC 4 cut(s) 153, 912, 1530, 1620
AccII CGCG 1 cut(s) 171
AclWI GGATC 3 cut(s) 76, 155, 590
AcoI YGGCCR 4 cut(s) 302, 315, 603, 904
AcsI RAATTY 2 cut(s) 1168, 1447
AcuI CTGAAG 1 cut(s) 1533
AcvI CACGTG 1 cut(s) 300
AcyI GRCGYC 2 cut(s) 210, 621
AfaI GTAC 3 cut(s) 692, 698, 1022
AfiI CCNNNNNNNGG 4 cut(s) 144, 451, 1214, 1268
AflII CTTAAG 1 cut(s) 502
AhdI GACNNNNNGTC 1 cut(s) 1193
AjiI CACGTC 2 cut(s) 270, 742
AjnI CCWGG 5 cut(s) 312, 444, 511, 1219, 1352
AluBI AGCT 9 cut(s) 119, 178, 440, 645, 658, 713, 1337, 1397, 1598
AluI AGCT 9 cut(s) 119, 178, 440, 645, 658, 713, 1337, 1397, 1598
Alw21I GWGCWC 2 cut(s) 726, 846
Alw26I GTCTC 3 cut(s) 118, 320, 1180
Alw44I GTGCAC 2 cut(s) 722, 842
AlwI GGATC 3 cut(s) 76, 155, 590
Ama87I CYCGRG 1 cut(s) 1658
ApaLI GTGCAC 2 cut(s) 722, 842
ApeKI GCWGC 8 cut(s) 166, 238, 525, 658, 1040, 1043, 1046, 1418
ApoI RAATTY 2 cut(s) 1168, 1447
AspLEI GCGC 2 cut(s) 171, 264
AspS9I GGNCC 8 cut(s) 665, 988, 1217, 1301, 1316, 1341, 1360, 1649
AsuHPI GGTGA 1 cut(s) 418
AsuNHI GCTAGC 1 cut(s) 1598
AvaI CYCGRG 1 cut(s) 1658
AvaII GGWCC 4 cut(s) 988, 1316, 1341, 1360
BaeGI GKGCMC 2 cut(s) 726, 846
BalI TGGCCA 2 cut(s) 317, 605
BbrPI CACGTG 1 cut(s) 300
Bbv12I GWGCWC 2 cut(s) 726, 846
BbvCI CCTCAGC 2 cut(s) 230, 714
BbvI GCAGC 8 cut(s) 178, 250, 537, 645, 1027, 1030, 1033, 1430
BccI CCATC 3 cut(s) 138, 475, 581
BceAI ACGGC 4 cut(s) 197, 553, 815, 1522
BcgI CGANNNNNNTGC 2 cut(s) 616, 650
BciT130I CCWGG 5 cut(s) 314, 446, 513, 1221, 1354
BciVI GTATCC 2 cut(s) 404, 1422
BcoDI GTCTC 3 cut(s) 118, 320, 1180
BfaI CTAG 3 cut(s) 459, 791, 1599
BfmI CTRYAG 1 cut(s) 1644
BfrI CTTAAG 1 cut(s) 502
BfuI GTATCC 2 cut(s) 404, 1422
Bme1390I CCNGG 5 cut(s) 314, 446, 513, 1221, 1354
Bme18I GGWCC 4 cut(s) 988, 1316, 1341, 1360
BmeRI GACNNNNNGTC 1 cut(s) 1193
BmeT110I CYCGRG 1 cut(s) 1658
BmgBI CACGTC 2 cut(s) 270, 742
BmgT120I GGNCC 8 cut(s) 665, 988, 1217, 1301, 1316, 1341, 1360, 1649
BmiI GGNNCC 4 cut(s) 667, 959, 1000, 1651
BmrFI CCNGG 5 cut(s) 314, 446, 513, 1221, 1354
BmrI ACTGGG 1 cut(s) 1156
BmsI GCATC 2 cut(s) 211, 273
BmtI GCTAGC 1 cut(s) 1602
BmuI ACTGGG 1 cut(s) 1156
BplI GAGNNNNNCTC 2 cut(s) 1652, 1684
BpmI CTGGAG 2 cut(s) 1353, 1519
Bpu10I CCTNAGC 2 cut(s) 230, 714
BpuEI CTTGAG 3 cut(s) 667, 784, 1591
Bsa29I ATCGAT 1 cut(s) 585
BsaAI YACGTR 1 cut(s) 300
BsaHI GRCGYC 2 cut(s) 210, 621
BsaJI CCNNGG 5 cut(s) 367, 463, 512, 1297, 1500
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 451, 1214, 1268
Bse118I RCCGGY 2 cut(s) 241, 1414
Bse1I ACTGG 6 cut(s) 397, 495, 559, 851, 1151, 1603
Bse3DI GCAATG 2 cut(s) 547, 634
BseBI CCWGG 5 cut(s) 314, 446, 513, 1221, 1354
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 5 cut(s) 367, 463, 512, 1297, 1500
BseGI GGATG 2 cut(s) 1258, 1349
BseLI CCNNNNNNNGG 4 cut(s) 144, 451, 1214, 1268
BseMI GCAATG 2 cut(s) 547, 634
BseMII CTCAG 6 cut(s) 129, 244, 705, 1196, 1340, 1683
BseNI ACTGG 6 cut(s) 397, 495, 559, 851, 1151, 1603
BseRI GAGGAG 2 cut(s) 127, 723
BseSI GKGCMC 2 cut(s) 726, 846
BseXI GCAGC 8 cut(s) 178, 250, 537, 645, 1027, 1030, 1033, 1430
BseYI CCCAGC 2 cut(s) 274, 1594
Bsh1236I CGCG 1 cut(s) 171
BshVI ATCGAT 1 cut(s) 585
BsiHKAI GWGCWC 2 cut(s) 726, 846
BsiHKCI CYCGRG 1 cut(s) 1658
BsiSI CCGG 3 cut(s) 242, 390, 1415
BslI CCNNNNNNNGG 4 cut(s) 144, 451, 1214, 1268
BsmAI GTCTC 3 cut(s) 118, 320, 1180
BsmBI CGTCTC 1 cut(s) 320
BsmI GAATGC 1 cut(s) 1245
BsoBI CYCGRG 1 cut(s) 1658
Bsp1286I GDGCHC 2 cut(s) 726, 846
Bsp143I GATC 5 cut(s) 81, 147, 421, 582, 1248
BspCNI CTCAG 6 cut(s) 128, 243, 706, 1195, 1339, 1682
BspDI ATCGAT 1 cut(s) 585
BspFNI CGCG 1 cut(s) 171
BspLI GGNNCC 4 cut(s) 667, 959, 1000, 1651
BspOI GCTAGC 1 cut(s) 1602
BspPI GGATC 3 cut(s) 76, 155, 590
BspTI CTTAAG 1 cut(s) 502
BsrBI CCGCTC 3 cut(s) 127, 882, 986
BsrDI GCAATG 2 cut(s) 547, 634
BsrFI RCCGGY 2 cut(s) 241, 1414
BsrI ACTGG 6 cut(s) 397, 495, 559, 851, 1151, 1603
BssAI RCCGGY 2 cut(s) 241, 1414
BssECI CCNNGG 5 cut(s) 367, 463, 512, 1297, 1500
BssMI GATC 5 cut(s) 81, 147, 421, 582, 1248
BssNAI GTATAC 1 cut(s) 913
BssNI GRCGYC 2 cut(s) 210, 621
BssT1I CCWWGG 2 cut(s) 463, 1297
Bst1107I GTATAC 1 cut(s) 913
Bst2UI CCWGG 5 cut(s) 314, 446, 513, 1221, 1354
Bst4CI ACNGT 3 cut(s) 346, 832, 1546
Bst6I CTCTTC 2 cut(s) 920, 1119
BstACI GRCGYC 2 cut(s) 210, 621
BstAFI CTTAAG 1 cut(s) 502
BstBAI YACGTR 1 cut(s) 300
BstC8I GCNNGC 9 cut(s) 108, 180, 184, 236, 333, 509, 870, 1416, 1600
BstDEI CTNAG 8 cut(s) 115, 230, 714, 1182, 1326, 1476, 1494, 1669
BstDSI CCRYGG 1 cut(s) 1500
BstENI CCTNNNNNAGG 1 cut(s) 1212
BstF5I GGATG 2 cut(s) 1258, 1349
BstFNI CGCG 1 cut(s) 171
BstHHI GCGC 2 cut(s) 171, 264
BstKTI GATC 5 cut(s) 84, 150, 424, 585, 1251
BstMAI GTCTC 3 cut(s) 118, 320, 1180
BstMBI GATC 5 cut(s) 81, 147, 421, 582, 1248
BstMWI GCNNNNNNNGC 8 cut(s) 116, 175, 218, 301, 446, 468, 874, 1046
BstNI CCWGG 5 cut(s) 314, 446, 513, 1221, 1354
BstNSI RCATGY 1 cut(s) 703
BstSCI CCNGG 5 cut(s) 312, 444, 511, 1219, 1352
BstSFI CTRYAG 1 cut(s) 1644
BstSLI GKGCMC 2 cut(s) 726, 846
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 8 cut(s) 178, 250, 537, 645, 1027, 1030, 1033, 1430
BstZ17I GTATAC 1 cut(s) 913
Bsu15I ATCGAT 1 cut(s) 585
BsuI GTATCC 2 cut(s) 404, 1422
BsuTUI ATCGAT 1 cut(s) 585
BtgI CCRYGG 1 cut(s) 1500
BtrI CACGTC 2 cut(s) 270, 742
BtsCI GGATG 2 cut(s) 1258, 1349
BtsI GCAGTG 3 cut(s) 456, 726, 1428
BtsIMutI CAGTG 7 cut(s) 342, 456, 726, 844, 1428, 1509, 1610
Cac8I GCNNGC 9 cut(s) 108, 180, 184, 236, 333, 509, 870, 1416, 1600
CfoI GCGC 2 cut(s) 171, 264
Cfr10I RCCGGY 2 cut(s) 241, 1414
Cfr13I GGNCC 8 cut(s) 665, 988, 1217, 1301, 1316, 1341, 1360, 1649
ClaI ATCGAT 1 cut(s) 585
CseI GACGC 5 cut(s) 218, 257, 277, 337, 610
Csp6I GTAC 3 cut(s) 691, 697, 1021
CviAII CATG 6 cut(s) 43, 640, 700, 873, 1036, 1320
CviQI GTAC 3 cut(s) 691, 697, 1021
DdeI CTNAG 8 cut(s) 115, 230, 714, 1182, 1326, 1476, 1494, 1669
DpnI GATC 5 cut(s) 83, 149, 423, 584, 1250
DpnII GATC 5 cut(s) 81, 147, 421, 582, 1248
DrdI GACNNNNNNGTC 1 cut(s) 746
DriI GACNNNNNGTC 1 cut(s) 1193
DseDI GACNNNNNNGTC 1 cut(s) 746
EaeI YGGCCR 4 cut(s) 302, 315, 603, 904
Eam1104I CTCTTC 2 cut(s) 920, 1119
Eam1105I GACNNNNNGTC 1 cut(s) 1193
EarI CTCTTC 2 cut(s) 920, 1119
Eco130I CCWWGG 2 cut(s) 463, 1297
Eco147I AGGCCT 2 cut(s) 228, 1272
Eco32I GATATC 2 cut(s) 1162, 1462
Eco47I GGWCC 4 cut(s) 988, 1316, 1341, 1360
Eco57I CTGAAG 1 cut(s) 1533
Eco72I CACGTG 1 cut(s) 300
Eco88I CYCGRG 1 cut(s) 1658
EcoNI CCTNNNNNAGG 1 cut(s) 1212
EcoO109I RGGNCCY 3 cut(s) 665, 1360, 1649
EcoRI GAATTC 2 cut(s) 1168, 1447
EcoRII CCWGG 5 cut(s) 312, 444, 511, 1219, 1352
EcoRV GATATC 2 cut(s) 1162, 1462
EcoT14I CCWWGG 2 cut(s) 463, 1297
ErhI CCWWGG 2 cut(s) 463, 1297
Esp3I CGTCTC 1 cut(s) 320
FaeI CATG 6 cut(s) 46, 643, 703, 876, 1039, 1323
FalI AAGNNNNNCTT 2 cut(s) 1506, 1538
FatI CATG 6 cut(s) 42, 639, 699, 872, 1035, 1319
FauI CCCGC 4 cut(s) 115, 132, 418, 814
FauNDI CATATG 1 cut(s) 1680
FblI GTMKAC 4 cut(s) 153, 912, 1530, 1620
FokI GGATG 2 cut(s) 1245, 1336
FspBI CTAG 3 cut(s) 459, 791, 1599
GlaI GCGC 2 cut(s) 170, 263
GsaI CCCAGC 2 cut(s) 278, 1598
GsuI CTGGAG 2 cut(s) 1353, 1519
HapII CCGG 3 cut(s) 242, 390, 1415
HgaI GACGC 5 cut(s) 218, 257, 277, 337, 610
HhaI GCGC 2 cut(s) 171, 264
Hin1I GRCGYC 2 cut(s) 210, 621
Hin1II CATG 6 cut(s) 46, 643, 703, 876, 1039, 1323
Hin6I GCGC 2 cut(s) 169, 262
HinP1I GCGC 2 cut(s) 169, 262
HincII GTYRAC 3 cut(s) 154, 682, 1621
HindII GTYRAC 3 cut(s) 154, 682, 1621
HindIII AAGCTT 1 cut(s) 438
HinfI GANTC 7 cut(s) 279, 380, 1078, 1137, 1293, 1472, 1532
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 3 cut(s) 242, 390, 1415
HphI GGTGA 1 cut(s) 418
Hpy188I TCNGA 5 cut(s) 363, 371, 862, 1185, 1471
Hpy99I CGWCG 7 cut(s) 155, 158, 212, 218, 274, 422, 539
HpyAV CCTTC 6 cut(s) 101, 218, 947, 1100, 1351, 1496
HpyCH4III ACNGT 3 cut(s) 346, 832, 1546
HpyCH4IV ACGT 5 cut(s) 269, 299, 741, 921, 1379
HpyF10VI GCNNNNNNNGC 8 cut(s) 116, 175, 218, 301, 446, 468, 874, 1046
HpyF3I CTNAG 8 cut(s) 115, 230, 714, 1182, 1326, 1476, 1494, 1669
HpySE526I ACGT 5 cut(s) 269, 299, 741, 921, 1379
Hsp92I GRCGYC 2 cut(s) 210, 621
Hsp92II CATG 6 cut(s) 46, 643, 703, 876, 1039, 1323
HspAI GCGC 2 cut(s) 169, 262
KroI GCCGGC 1 cut(s) 1414
KroNI GCCGGC 1 cut(s) 1416
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 5 cut(s) 81, 147, 421, 582, 1248
LmnI GCTCC 4 cut(s) 468, 710, 983, 1334
Lsp1109I GCAGC 8 cut(s) 178, 250, 537, 645, 1027, 1030, 1033, 1430
LweI GCATC 2 cut(s) 211, 273
MaeI CTAG 3 cut(s) 459, 791, 1599
MaeII ACGT 5 cut(s) 269, 299, 741, 921, 1379
MaeIII GTNAC 2 cut(s) 340, 1549
MalI GATC 5 cut(s) 83, 149, 423, 584, 1250
MbiI CCGCTC 3 cut(s) 127, 882, 986
MboI GATC 5 cut(s) 81, 147, 421, 582, 1248
MboII GAAGA 3 cut(s) 937, 962, 1136
MhlI GDGCHC 2 cut(s) 726, 846
MlsI TGGCCA 2 cut(s) 317, 605
MluCI AATT 4 cut(s) 190, 1168, 1447, 1582
MluNI TGGCCA 2 cut(s) 317, 605
MlyI GAGTC 2 cut(s) 1072, 1526
MmeI TCCRAC 2 cut(s) 426, 1270
Mox20I TGGCCA 2 cut(s) 317, 605
MroNI GCCGGC 1 cut(s) 1414
MscI TGGCCA 2 cut(s) 317, 605
MseI TTAA 3 cut(s) 503, 681, 1025
MslI CAYNNNNRTG 1 cut(s) 849
Msp20I TGGCCA 2 cut(s) 317, 605
MspCI CTTAAG 1 cut(s) 502
MspI CCGG 3 cut(s) 242, 390, 1415
MspR9I CCNGG 5 cut(s) 314, 446, 513, 1221, 1354
Mva1269I GAATGC 1 cut(s) 1245
MvaI CCWGG 5 cut(s) 314, 446, 513, 1221, 1354
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 8 cut(s) 116, 175, 218, 301, 446, 468, 874, 1046
NaeI GCCGGC 1 cut(s) 1416
NdeI CATATG 1 cut(s) 1680
NdeII GATC 5 cut(s) 81, 147, 421, 582, 1248
NgoMIV GCCGGC 1 cut(s) 1414
NheI GCTAGC 1 cut(s) 1598
NlaIII CATG 6 cut(s) 46, 643, 703, 876, 1039, 1323
NlaIV GGNNCC 4 cut(s) 667, 959, 1000, 1651
NmuCI GTSAC 2 cut(s) 340, 1549
NspI RCATGY 1 cut(s) 703
PaeR7I CTCGAG 1 cut(s) 1658
PceI AGGCCT 2 cut(s) 228, 1272
PcsI WCGNNNNNNNCGW 3 cut(s) 213, 375, 1634
PctI GAATGC 1 cut(s) 1245
PdiI GCCGGC 1 cut(s) 1416
PfeI GAWTC 5 cut(s) 279, 380, 1137, 1293, 1472
PflFI GACNNNGTC 1 cut(s) 212
PflMI CCANNNNNTGG 1 cut(s) 451
PfoI TCCNGGA 1 cut(s) 1352
PleI GAGTC 2 cut(s) 1072, 1526
PmaCI CACGTG 1 cut(s) 300
PmlI CACGTG 1 cut(s) 300
PpsI GAGTC 2 cut(s) 1072, 1526
Ppu21I YACGTR 1 cut(s) 300
PpuMI RGGWCCY 1 cut(s) 1360
Psp5II RGGWCCY 1 cut(s) 1360
Psp6I CCWGG 5 cut(s) 312, 444, 511, 1219, 1352
PspCI CACGTG 1 cut(s) 300
PspFI CCCAGC 2 cut(s) 274, 1594
PspGI CCWGG 5 cut(s) 312, 444, 511, 1219, 1352
PspN4I GGNNCC 4 cut(s) 667, 959, 1000, 1651
PspPI GGNCC 8 cut(s) 665, 988, 1217, 1301, 1316, 1341, 1360, 1649
PspPPI RGGWCCY 1 cut(s) 1360
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 3 cut(s) 692, 698, 1022
RsaNI GTAC 3 cut(s) 691, 697, 1021
RseI CAYNNNNRTG 1 cut(s) 849
SalI GTCGAC 2 cut(s) 152, 1619
SaqAI TTAA 3 cut(s) 503, 681, 1025
Sau3AI GATC 5 cut(s) 81, 147, 421, 582, 1248
Sau96I GGNCC 8 cut(s) 665, 988, 1217, 1301, 1316, 1341, 1360, 1649
SchI GAGTC 2 cut(s) 1072, 1526
ScrFI CCNGG 5 cut(s) 314, 446, 513, 1221, 1354
SduI GDGCHC 2 cut(s) 726, 846
SfaNI GCATC 2 cut(s) 211, 273
SfcI CTRYAG 1 cut(s) 1644
Sfr274I CTCGAG 1 cut(s) 1658
SgrDI CGTCGACG 1 cut(s) 152
SinI GGWCC 4 cut(s) 988, 1316, 1341, 1360
SlaI CTCGAG 1 cut(s) 1658
SmiMI CAYNNNNRTG 1 cut(s) 849
SmlI CTYRAG 5 cut(s) 502, 646, 763, 1570, 1658
SmoI CTYRAG 5 cut(s) 502, 646, 763, 1570, 1658
Sse9I AATT 4 cut(s) 190, 1168, 1447, 1582
SseBI AGGCCT 2 cut(s) 228, 1272
SspMI CTAG 3 cut(s) 459, 791, 1599
StuI AGGCCT 2 cut(s) 228, 1272
StyD4I CCNGG 5 cut(s) 312, 444, 511, 1219, 1352
StyI CCWWGG 2 cut(s) 463, 1297
TaaI ACNGT 3 cut(s) 346, 832, 1546
TaiI ACGT 5 cut(s) 272, 302, 744, 924, 1382
TaqI TCGA 9 cut(s) 80, 153, 207, 378, 420, 585, 598, 1620, 1659
TasI AATT 4 cut(s) 190, 1168, 1447, 1582
TatI WGTACW 1 cut(s) 696
TauI GCSGC 4 cut(s) 307, 338, 885, 906
TfiI GAWTC 5 cut(s) 279, 380, 1137, 1293, 1472
Tru1I TTAA 3 cut(s) 503, 681, 1025
Tru9I TTAA 3 cut(s) 503, 681, 1025
TscAI CASTG 7 cut(s) 349, 456, 726, 851, 1428, 1516, 1610
TseFI GTSAC 2 cut(s) 340, 1549
TseI GCWGC 8 cut(s) 166, 238, 525, 658, 1040, 1043, 1046, 1418
Tsp45I GTSAC 2 cut(s) 340, 1549
TspDTI ATGAA 4 cut(s) 17, 1024, 1247, 1338
TspGWI ACGGA 2 cut(s) 791, 1517
TspRI CASTG 7 cut(s) 349, 456, 726, 851, 1428, 1516, 1610
Tth111I GACNNNGTC 1 cut(s) 212
Van91I CCANNNNNTGG 1 cut(s) 451
Vha464I CTTAAG 1 cut(s) 502
VneI GTGCAC 2 cut(s) 722, 842
VpaK11BI GGWCC 4 cut(s) 988, 1316, 1341, 1360
XagI CCTNNNNNAGG 1 cut(s) 1212
XapI RAATTY 2 cut(s) 1168, 1447
XceI RCATGY 1 cut(s) 703
XcmI CCANNNNNNNNNTGG 2 cut(s) 328, 1602
XhoI CTCGAG 1 cut(s) 1658
XmiI GTMKAC 4 cut(s) 153, 912, 1530, 1620
XspI CTAG 3 cut(s) 459, 791, 1599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.