Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007900

Physical Location & Seq

Genomic Coordinates Forward (+)

7819581 .. 7823275

Gene Structure 3695 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1111400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGAATGGAACGGTGGTACGTGATGTGGAAATGGATGGGTTTTTAGGGTATTTGCAGGAGCAGAAGAAAGTAGCCATTGAAGAACAGGAGCCCAATCTGCATATCTACTCAAGCAGAGATGTGGACATGATTCTGAATGATGAGGATGTAGATATAAAGGAAGAGGTTCCTGGCACTCCACCATCCGAATCCCAGAACCGTTACTCAGAGCCGTCTGACTCAACGCCACAAACAATTGGGAACGTTCCTTCTTCTGGAAAACCATCAAGACTTGTAACTCCTTTTGGGCAACGAACAGACAAGTTTGTTGTGAAATTCAGCATTGATAATAAGCCAACTATAGAGAACGGGGAGAAGGAACATGATAATGAGAATGATGAGGATGATGAAATTATCAGGAAGGTTAAGCCTGGAAAGCGCTGTTCTTTTGTAGTCCATGGTTCAGGACCTGAAATAGGTTGTAGGTTTATGTATGATAGGATTGAAGATAGGTTTAACGCACTTGAAAACCGAATTAGAAAGTACGCAACTGCATTAGTTTCATCTCGGCAGTACGAGGAACCGCAGGACCCTACAGTTTCGTCTCAGAAAACTATATTTACTGCTGGCATGGTCTGTTGTGACGGAGAGGGCCATTTGAATGACAAATCCACCTTGTTGCAGAGCAGCGCAGAGCATTCTGGAGGTCAACGTGTTCGTATGGAATTACAAAATTTGAGCCAATTTTCAATCTTTCCAGGCCAGATAATCGGTGTCGAAGGGACCAATCCTAGTGGACACTGCTTCATTGCTTCAAAACTGGTAGATTCAGTCCCTCTACCTGTTGCTGCTGATGCAAACTCGCCTCCTGCTAAAAAGCAGGCTTTGGATCAGGAGATTTTGCCAACTGAACTTTCCAGTAAACAACCAGAGCTATCAGTGATCATCGCATCAGGCCCTTTTACCACAACAGACAACTTATTTTTTGAGCCTCTGAGAGAGCTTCTAGCATATGCAAGCAAAAAGCTGCCTCAATTGCTTATATTGCTAGGACCATTTATTGATTCTGAACATCCAGAAATTAAGAAAGGAACCGTTGACAGGAGCTTTGATGAAATATTCCAAACTGAGATTCTTAGAAGGTTGCAAGATCATGTCGAATACATGGGTTCGCATGCGCGCGTTGTTCTTGTACCATCCATACGGGATGCTAACCATGATTTTATATTCCCTCAGCCTGCCTTTGATATTCACCCACCTGATCTCAAGCATCAGATAAGCAGTCTCACAAACCCAGGGTTTTTCGAGGCAGATCAGGTCAAGATAGGTTGCTGCTCTGTGGATGTTCTCAAACACCTTAGTGCAGAGGAGATCTCGCGTGTTCCAAAGGATGGAAAACCCAGTGACCGCTTGAAGCTCTGCAATCTAATATTTTGCAATTTTTCGGACAGCTTTTATCCTCTGTATCCACCAGCAGAAGACATTCCACTGGATTTTTCACTTGCTCCTCAAGCTCTAAACATCTCATTGATCCCAAATATTCTCATCTTACCCTCTGACGTGAAGTATTTTGTGAAGGTACTGTCCCTTGGTGAAAGAGGTGAAGATAAAGAGCAGTTAAAATGCATTTGTGTCAATCCGGGAAGACTGGCCAAGGGAGAAGGAGGAGGTACTTTCGTCGAGCTTGACTACTATGGTAAGCCCGACACAACGAATGCTTCGATTATAAGCATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000082 GO:0000228 GO:0000278 GO:0000428 GO:0000723 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005658 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0019725 GO:0022402 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032991 GO:0032993 GO:0033260 GO:0034641 GO:0034645 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044786 GO:0044843 GO:0046483 GO:0046982 GO:0046983 GO:0051276 GO:0055029 GO:0060249 GO:0061695 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576 GO:1902494 GO:1903047 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_E_B Pol_alpha_B_N

Protein Analysis

572

Amino Acids

63.45

Weight (kDa)

4.96

Isoelectric Point (pI)

50.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
OB_DPOA2	PF22062	167 - 268	4.3e-24	DNA polymerase alpha subunit B, OB domain
DNA_pol_E_B	PF04042	307 - 519	2.8e-40	DNA polymerase alpha/epsilon subunit B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67630
fragaria_vesca	FvH4_4g24380 FvH4_4g24380 FvH4_4g24380 FvH4_4g24380 FvH4_4g24380 FvH4_4g24380 FvH4_4g24380
malus_domestica	MD13G1111800.v1.1 MD16G1111400.v1.1
prunus_persica	Prupe.1G233700_v2.0.a1 Prupe.1G233700_v2.0.a1
pyrus_communis	pycom13g09720 pycom16g09440
rosa_chinensis	RchiOBHm_Chr4g0431221
rosa_laevigata	RLG00000006918
rosa_multiflora	Rmu_sc0005258.1_g000007
rosa_roxburghii	Rroxscaffold_5G00372760
rosa_rugosa	Rorug04G0249200 Rorug04G0249200
rosa_samantha	Rh4BG312700 Rh4CG329000 Rh4DG309300
rosa_wichuraiana	Rw4G026540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1706
AccB7I	CCANNNNNTGG	1 cut(s)	1370
AccII	CGCG	3 cut(s)	1159, 1161, 1357
AciI	CCGC	2 cut(s)	563, 1387
AclI	AACGTT	1 cut(s)	243
AclWI	GGATC	2 cut(s)	876, 1504
AcoI	YGGCCR	1 cut(s)	1629
AcsI	RAATTY	2 cut(s)	314, 712
AfaI	GTAC	6 cut(s)	18, 524, 554, 1173, 1560, 1651
AfeI	AGCGCT	1 cut(s)	419
AfiI	CCNNNNNNNGG	4 cut(s)	254, 455, 1080, 1370
AflIII	ACRYGT	1 cut(s)	691
AgsI	TTSAA	7 cut(s)	80, 485, 506, 640, 729, 795, 1393
AjiI	CACGTC	1 cut(s)	1540
AjnI	CCWGG	4 cut(s)	169, 409, 736, 1273
AjuI	GAANNNNNNNTTGG	4 cut(s)	877, 909, 1095, 1127
AleI	CACNNNNGTG	1 cut(s)	1338
AluBI	AGCT	8 cut(s)	913, 982, 1006, 1086, 1396, 1431, 1493, 1663
AluI	AGCT	8 cut(s)	913, 982, 1006, 1086, 1396, 1431, 1493, 1663
Alw26I	GTCTC	2 cut(s)	588, 1268
AlwI	GGATC	2 cut(s)	876, 1504
AlwNI	CAGNNNCTG	1 cut(s)	449
Aor51HI	AGCGCT	1 cut(s)	419
AoxI	GGCC	4 cut(s)	631, 739, 934, 1629
ApeKI	GCWGC	4 cut(s)	666, 827, 1006, 1311
ApoI	RAATTY	2 cut(s)	314, 712
ArsI	GACNNNNNNTTYG	2 cut(s)	1070, 1102
Asp700I	GAANNNNTTC	2 cut(s)	165, 1461
AspLEI	GCGC	4 cut(s)	420, 671, 1159, 1161
AspS9I	GGNCC	6 cut(s)	446, 568, 631, 762, 935, 1031
AsuC2I	CCSGG	1 cut(s)	1620
AsuHPI	GGTGA	3 cut(s)	1223, 1583, 1592
AvaII	GGWCC	4 cut(s)	446, 568, 762, 1031
BaeI	ACNNNNGTAYC	2 cut(s)	1641, 1674
BalI	TGGCCA	1 cut(s)	1631
BanII	GRGCYC	1 cut(s)	93
BbsI	GAAGAC	2 cut(s)	1464, 1630
BbvCI	CCTCAGC	1 cut(s)	1212
BbvI	GCAGC	4 cut(s)	678, 814, 993, 1298
BccI	CCATC	5 cut(s)	29, 190, 271, 1183, 1364
BceAI	ACGGC	1 cut(s)	196
BciT130I	CCWGG	4 cut(s)	171, 411, 738, 1275
BciVI	GTATCC	1 cut(s)	1455
BclI	TGATCA	1 cut(s)	921
BcnI	CCSGG	1 cut(s)	1620
BcoDI	GTCTC	2 cut(s)	588, 1268
BfaI	CTAG	3 cut(s)	771, 986, 1028
BfmI	CTRYAG	2 cut(s)	339, 573
BfoI	RGCGCY	1 cut(s)	421
BfuI	GTATCC	1 cut(s)	1455
BglII	AGATCT	1 cut(s)	1350
BisI	GCNGC	4 cut(s)	667, 828, 1007, 1312
BlsI	GCNGC	4 cut(s)	668, 829, 1008, 1313
Bme1390I	CCNGG	5 cut(s)	171, 411, 738, 1275, 1620
Bme18I	GGWCC	4 cut(s)	446, 568, 762, 1031
BmgBI	CACGTC	1 cut(s)	1540
BmgT120I	GGNCC	6 cut(s)	446, 568, 631, 762, 935, 1031
BmiI	GGNNCC	6 cut(s)	90, 168, 561, 570, 763, 1072
BmrFI	CCNGG	5 cut(s)	171, 411, 738, 1275, 1620
BmrI	ACTGGG	1 cut(s)	1374
BmsI	GCATC	4 cut(s)	823, 938, 1177, 1258
BmuI	ACTGGG	1 cut(s)	1374
BpiI	GAAGAC	2 cut(s)	1464, 1630
BplI	GAGNNNNNCTC	2 cut(s)	1337, 1369
BpmI	CTGGAG	1 cut(s)	702
Bpu10I	CCTNAGC	1 cut(s)	1212
BpuEI	CTTGAG	3 cut(s)	94, 1229, 1473
BpuMI	CCSGG	1 cut(s)	1620
BsaAI	YACGTR	1 cut(s)	20
BsaJI	CCNNGG	5 cut(s)	436, 1273, 1274, 1567, 1632
Bsc4I	CCNNNNNNNGG	4 cut(s)	254, 455, 1080, 1370
Bse1I	ACTGG	5 cut(s)	804, 897, 1380, 1473, 1632
Bse3DI	GCAATG	1 cut(s)	786
BseBI	CCWGG	4 cut(s)	171, 411, 738, 1275
BseDI	CCNNGG	5 cut(s)	436, 1273, 1274, 1567, 1632
BseGI	GGATG	9 cut(s)	40, 151, 182, 388, 1051, 1175, 1192, 1327, 1375
BseLI	CCNNNNNNNGG	4 cut(s)	254, 455, 1080, 1370
BseMI	GCAATG	1 cut(s)	786
BseMII	CTCAG	5 cut(s)	219, 599, 965, 1098, 1226
BseNI	ACTGG	5 cut(s)	804, 897, 1380, 1473, 1632
BsePI	GCGCGC	1 cut(s)	1157
BseRI	GAGGAG	3 cut(s)	1361, 1476, 1659
BseXI	GCAGC	4 cut(s)	678, 814, 993, 1298
BsgI	GTGCAG	1 cut(s)	1362
Bsh1236I	CGCG	3 cut(s)	1159, 1161, 1357
BshFI	GGCC	4 cut(s)	633, 741, 936, 1631
BsiSI	CCGG	1 cut(s)	1619
BslFI	GGGAC	3 cut(s)	775, 797, 1549
BslI	CCNNNNNNNGG	4 cut(s)	254, 455, 1080, 1370
BsmAI	GTCTC	2 cut(s)	588, 1268
BsmBI	CGTCTC	1 cut(s)	588
BsmFI	GGGAC	3 cut(s)	775, 797, 1549
BsmI	GAATGC	2 cut(s)	676, 1699
BsnI	GGCC	4 cut(s)	633, 741, 936, 1631
Bsp1286I	GDGCHC	1 cut(s)	93
Bsp143I	GATC	7 cut(s)	868, 921, 1129, 1240, 1291, 1350, 1509
Bsp19I	CCATGG	1 cut(s)	436
BspACI	CCGC	2 cut(s)	563, 1387
BspANI	GGCC	4 cut(s)	633, 741, 936, 1631
BspCNI	CTCAG	5 cut(s)	218, 598, 966, 1099, 1225
BspFNI	CGCG	3 cut(s)	1159, 1161, 1357
BspLI	GGNNCC	6 cut(s)	90, 168, 561, 570, 763, 1072
BspPI	GGATC	2 cut(s)	876, 1504
BsrDI	GCAATG	1 cut(s)	786
BsrI	ACTGG	5 cut(s)	804, 897, 1380, 1473, 1632
BssECI	CCNNGG	5 cut(s)	436, 1273, 1274, 1567, 1632
BssHII	GCGCGC	1 cut(s)	1157
BssMI	GATC	7 cut(s)	868, 921, 1129, 1240, 1291, 1350, 1509
BssT1I	CCWWGG	3 cut(s)	436, 1567, 1632
Bst2UI	CCWGG	4 cut(s)	171, 411, 738, 1275
Bst4CI	ACNGT	5 cut(s)	13, 200, 577, 1075, 1563
Bst6I	CTCTTC	1 cut(s)	156
BstBAI	YACGTR	1 cut(s)	20
BstC8I	GCNNGC	6 cut(s)	607, 861, 997, 1155, 1159, 1218
BstDEI	CTNAG	7 cut(s)	205, 585, 974, 1107, 1115, 1212, 1337
BstDSI	CCRYGG	1 cut(s)	436
BstENI	CCTNNNNNAGG	1 cut(s)	453
BstF5I	GGATG	9 cut(s)	40, 151, 182, 388, 1051, 1175, 1192, 1327, 1375
BstFNI	CGCG	3 cut(s)	1159, 1161, 1357
BstH2I	RGCGCY	1 cut(s)	421
BstHHI	GCGC	4 cut(s)	420, 671, 1159, 1161
BstKTI	GATC	7 cut(s)	871, 924, 1132, 1243, 1294, 1353, 1512
BstMAI	GTCTC	2 cut(s)	588, 1268
BstMBI	GATC	7 cut(s)	868, 921, 1129, 1240, 1291, 1350, 1509
BstMWI	GCNNNNNNNGC	6 cut(s)	97, 415, 833, 1015, 1024, 1490
BstNI	CCWGG	4 cut(s)	171, 411, 738, 1275
BstNSI	RCATGY	1 cut(s)	1157
BstSCI	CCNGG	5 cut(s)	169, 409, 736, 1273, 1618
BstSFI	CTRYAG	2 cut(s)	339, 573
BstUI	CGCG	3 cut(s)	1159, 1161, 1357
BstV1I	GCAGC	4 cut(s)	678, 814, 993, 1298
BstV2I	GAAGAC	2 cut(s)	1464, 1630
BstX2I	RGATCY	1 cut(s)	1350
BstYI	RGATCY	1 cut(s)	1350
BsuI	GTATCC	1 cut(s)	1455
BsuRI	GGCC	4 cut(s)	633, 741, 936, 1631
BtgI	CCRYGG	1 cut(s)	436
BtgZI	GCGATG	1 cut(s)	910
BtrI	CACGTC	1 cut(s)	1540
BtsCI	GGATG	9 cut(s)	40, 151, 182, 388, 1051, 1175, 1192, 1327, 1375
BtsI	GCAGTG	1 cut(s)	778
BtsIMutI	CAGTG	4 cut(s)	778, 924, 1387, 1466
Cac8I	GCNNGC	6 cut(s)	607, 861, 997, 1155, 1159, 1218
CaiI	CAGNNNCTG	1 cut(s)	449
CfoI	GCGC	4 cut(s)	420, 671, 1159, 1161
Cfr13I	GGNCC	6 cut(s)	446, 568, 631, 762, 935, 1031
Csp6I	GTAC	6 cut(s)	17, 523, 553, 1172, 1559, 1650
CspCI	CAANNNNNGTGG	2 cut(s)	754, 789
CviAII	CATG	8 cut(s)	127, 362, 437, 610, 1133, 1144, 1154, 1196
CviQI	GTAC	6 cut(s)	17, 523, 553, 1172, 1559, 1650
DdeI	CTNAG	7 cut(s)	205, 585, 974, 1107, 1115, 1212, 1337
DpnI	GATC	7 cut(s)	870, 923, 1131, 1242, 1293, 1352, 1511
DpnII	GATC	7 cut(s)	868, 921, 1129, 1240, 1291, 1350, 1509
EaeI	YGGCCR	1 cut(s)	1629
Eam1104I	CTCTTC	1 cut(s)	156
EarI	CTCTTC	1 cut(s)	156
Eco130I	CCWWGG	3 cut(s)	436, 1567, 1632
Eco24I	GRGCYC	1 cut(s)	93
Eco47I	GGWCC	4 cut(s)	446, 568, 762, 1031
Eco47III	AGCGCT	1 cut(s)	419
EcoNI	CCTNNNNNAGG	1 cut(s)	453
EcoO109I	RGGNCCY	3 cut(s)	446, 568, 935
EcoRII	CCWGG	4 cut(s)	169, 409, 736, 1273
EcoT14I	CCWWGG	3 cut(s)	436, 1567, 1632
EcoT22I	ATGCAT	1 cut(s)	1607
EcoT38I	GRGCYC	1 cut(s)	93
ErhI	CCWWGG	3 cut(s)	436, 1567, 1632
Esp3I	CGTCTC	1 cut(s)	588
FaeI	CATG	8 cut(s)	130, 365, 440, 613, 1136, 1147, 1157, 1199
FaqI	GGGAC	3 cut(s)	775, 797, 1549
FatI	CATG	8 cut(s)	126, 361, 436, 609, 1132, 1143, 1153, 1195
FauNDI	CATATG	1 cut(s)	991
FbaI	TGATCA	1 cut(s)	921
Fnu4HI	GCNGC	4 cut(s)	667, 828, 1007, 1312
FokI	GGATG	9 cut(s)	47, 158, 169, 395, 1038, 1162, 1199, 1334, 1382
FriOI	GRGCYC	1 cut(s)	93
Fsp4HI	GCNGC	4 cut(s)	667, 828, 1007, 1312
FspBI	CTAG	3 cut(s)	771, 986, 1028
GlaI	GCGC	4 cut(s)	419, 670, 1158, 1160
GluI	GCNGC	4 cut(s)	667, 828, 1007, 1312
GsuI	CTGGAG	1 cut(s)	702
HaeII	RGCGCY	1 cut(s)	421
HaeIII	GGCC	4 cut(s)	633, 741, 936, 1631
HapII	CCGG	1 cut(s)	1619
HhaI	GCGC	4 cut(s)	420, 671, 1159, 1161
Hin1II	CATG	8 cut(s)	130, 365, 440, 613, 1136, 1147, 1157, 1199
Hin6I	GCGC	4 cut(s)	418, 669, 1157, 1159
HinP1I	GCGC	4 cut(s)	418, 669, 1157, 1159
HincII	GTYRAC	2 cut(s)	689, 1078
HindII	GTYRAC	2 cut(s)	689, 1078
HinfI	GANTC	6 cut(s)	130, 188, 218, 806, 1043, 1111
HpaII	CCGG	1 cut(s)	1619
HphI	GGTGA	3 cut(s)	1223, 1583, 1592
Hpy166II	GTNNAC	5 cut(s)	124, 689, 776, 902, 1078
Hpy188III	TCNNGA	8 cut(s)	255, 267, 397, 444, 681, 872, 1055, 1300
Hpy8I	GTNNAC	5 cut(s)	124, 689, 776, 902, 1078
Hpy99I	CGWCG	1 cut(s)	1661
HpyAV	CCTTC	7 cut(s)	258, 349, 394, 752, 1113, 1549, 1634
HpyCH4III	ACNGT	5 cut(s)	13, 200, 577, 1075, 1563
HpyCH4IV	ACGT	4 cut(s)	19, 243, 691, 1539
HpyF10VI	GCNNNNNNNGC	6 cut(s)	97, 415, 833, 1015, 1024, 1490
HpyF3I	CTNAG	7 cut(s)	205, 585, 974, 1107, 1115, 1212, 1337
HpySE526I	ACGT	4 cut(s)	19, 243, 691, 1539
Hsp92II	CATG	8 cut(s)	130, 365, 440, 613, 1136, 1147, 1157, 1199
HspAI	GCGC	4 cut(s)	418, 669, 1157, 1159
Ksp22I	TGATCA	1 cut(s)	921
Kzo9I	GATC	7 cut(s)	868, 921, 1129, 1240, 1291, 1350, 1509
LmnI	GCTCC	4 cut(s)	58, 88, 1083, 1489
Lsp1109I	GCAGC	4 cut(s)	678, 814, 993, 1298
LweI	GCATC	4 cut(s)	823, 938, 1177, 1258
MaeI	CTAG	3 cut(s)	771, 986, 1028
MaeII	ACGT	4 cut(s)	19, 243, 691, 1539
MaeIII	GTNAC	4 cut(s)	200, 274, 620, 1382
MalI	GATC	7 cut(s)	870, 923, 1131, 1242, 1293, 1352, 1511
MboI	GATC	7 cut(s)	868, 921, 1129, 1240, 1291, 1350, 1509
MboII	GAAGA	8 cut(s)	76, 92, 173, 243, 497, 1469, 1595, 1635
MfeI	CAATTG	2 cut(s)	234, 1013
MflI	RGATCY	1 cut(s)	1350
MhlI	GDGCHC	1 cut(s)	93
MlsI	TGGCCA	1 cut(s)	1631
MluNI	TGGCCA	1 cut(s)	1631
MlyI	GAGTC	1 cut(s)	212
Mox20I	TGGCCA	1 cut(s)	1631
Mph1103I	ATGCAT	1 cut(s)	1607
MroXI	GAANNNNTTC	2 cut(s)	165, 1461
MscI	TGGCCA	1 cut(s)	1631
MseI	TTAA	4 cut(s)	405, 495, 1062, 1598
MslI	CAYNNNNRTG	3 cut(s)	366, 639, 1338
Msp20I	TGGCCA	1 cut(s)	1631
MspI	CCGG	1 cut(s)	1619
MspR9I	CCNGG	5 cut(s)	171, 411, 738, 1275, 1620
MunI	CAATTG	2 cut(s)	234, 1013
Mva1269I	GAATGC	2 cut(s)	676, 1699
MvaI	CCWGG	4 cut(s)	171, 411, 738, 1275
MvnI	CGCG	3 cut(s)	1159, 1161, 1357
MwoI	GCNNNNNNNGC	6 cut(s)	97, 415, 833, 1015, 1024, 1490
NciI	CCSGG	1 cut(s)	1620
NcoI	CCATGG	1 cut(s)	436
NdeI	CATATG	1 cut(s)	991
NdeII	GATC	7 cut(s)	868, 921, 1129, 1240, 1291, 1350, 1509
NlaIII	CATG	8 cut(s)	130, 365, 440, 613, 1136, 1147, 1157, 1199
NlaIV	GGNNCC	6 cut(s)	90, 168, 561, 570, 763, 1072
NmeAIII	GCCGAG	1 cut(s)	526
NmuCI	GTSAC	2 cut(s)	620, 1382
NsiI	ATGCAT	1 cut(s)	1607
NspI	RCATGY	1 cut(s)	1157
OliI	CACNNNNGTG	1 cut(s)	1338
PaeI	GCATGC	1 cut(s)	1157
PasI	CCCWGGG	1 cut(s)	1274
PauI	GCGCGC	1 cut(s)	1157
PcsI	WCGNNNNNNNCGW	1 cut(s)	1697
PctI	GAATGC	2 cut(s)	676, 1699
PdmI	GAANNNNTTC	2 cut(s)	165, 1461
PfeI	GAWTC	5 cut(s)	130, 188, 806, 1043, 1111
PflMI	CCANNNNNTGG	1 cut(s)	1370
PfoI	TCCNGGA	1 cut(s)	1618
PkrI	GCNGC	4 cut(s)	668, 829, 1008, 1313
PleI	GAGTC	1 cut(s)	212
PpsI	GAGTC	1 cut(s)	212
Ppu21I	YACGTR	1 cut(s)	20
PpuMI	RGGWCCY	2 cut(s)	446, 568
PsiI	TTATAA	1 cut(s)	1706
Psp1406I	AACGTT	1 cut(s)	243
Psp5II	RGGWCCY	2 cut(s)	446, 568
Psp6I	CCWGG	4 cut(s)	169, 409, 736, 1273
PspGI	CCWGG	4 cut(s)	169, 409, 736, 1273
PspN4I	GGNNCC	6 cut(s)	90, 168, 561, 570, 763, 1072
PspPI	GGNCC	6 cut(s)	446, 568, 631, 762, 935, 1031
PspPPI	RGGWCCY	2 cut(s)	446, 568
PstNI	CAGNNNCTG	1 cut(s)	449
PsuI	RGATCY	1 cut(s)	1350
PteI	GCGCGC	1 cut(s)	1157
RsaI	GTAC	6 cut(s)	18, 524, 554, 1173, 1560, 1651
RsaNI	GTAC	6 cut(s)	17, 523, 553, 1172, 1559, 1650
RseI	CAYNNNNRTG	3 cut(s)	366, 639, 1338
SaqAI	TTAA	4 cut(s)	405, 495, 1062, 1598
SatI	GCNGC	4 cut(s)	667, 828, 1007, 1312
Sau3AI	GATC	7 cut(s)	868, 921, 1129, 1240, 1291, 1350, 1509
Sau96I	GGNCC	6 cut(s)	446, 568, 631, 762, 935, 1031
SchI	GAGTC	1 cut(s)	212
ScrFI	CCNGG	5 cut(s)	171, 411, 738, 1275, 1620
SduI	GDGCHC	1 cut(s)	93
SfaNI	GCATC	4 cut(s)	823, 938, 1177, 1258
SfcI	CTRYAG	2 cut(s)	339, 573
SinI	GGWCC	4 cut(s)	446, 568, 762, 1031
SmiMI	CAYNNNNRTG	3 cut(s)	366, 639, 1338
SmlI	CTYRAG	3 cut(s)	109, 1244, 1488
SmoI	CTYRAG	3 cut(s)	109, 1244, 1488
SphI	GCATGC	1 cut(s)	1157
SsiI	CCGC	2 cut(s)	563, 1387
SspI	AATATT	3 cut(s)	1098, 1410, 1519
SspMI	CTAG	3 cut(s)	771, 986, 1028
StyD4I	CCNGG	5 cut(s)	169, 409, 736, 1273, 1618
StyI	CCWWGG	3 cut(s)	436, 1567, 1632
TaaI	ACNGT	5 cut(s)	13, 200, 577, 1075, 1563
TaiI	ACGT	4 cut(s)	22, 246, 694, 1542
TaqI	TCGA	5 cut(s)	756, 1137, 1284, 1659, 1700
TfiI	GAWTC	5 cut(s)	130, 188, 806, 1043, 1111
Tru1I	TTAA	4 cut(s)	405, 495, 1062, 1598
Tru9I	TTAA	4 cut(s)	405, 495, 1062, 1598
TscAI	CASTG	4 cut(s)	785, 924, 1387, 1473
TseFI	GTSAC	2 cut(s)	620, 1382
TseI	GCWGC	4 cut(s)	666, 827, 1006, 1311
Tsp45I	GTSAC	2 cut(s)	620, 1382
TspDTI	ATGAA	5 cut(s)	17, 402, 531, 775, 1107
TspGWI	ACGGA	1 cut(s)	639
TspRI	CASTG	4 cut(s)	785, 924, 1387, 1473
Van91I	CCANNNNNTGG	1 cut(s)	1370
VpaK11BI	GGWCC	4 cut(s)	446, 568, 762, 1031
XagI	CCTNNNNNAGG	1 cut(s)	453
XapI	RAATTY	2 cut(s)	314, 712
XceI	RCATGY	1 cut(s)	1157
XmnI	GAANNNNTTC	2 cut(s)	165, 1461
XspI	CTAG	3 cut(s)	771, 986, 1028
Zsp2I	ATGCAT	1 cut(s)	1607

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.