Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0011550

Physical Location & Seq

Genomic Coordinates Reverse (-)

18855523 .. 18859111

Gene Structure 3589 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1204600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3045 bp

ATGGCAGTGGAGCTTATACCTATTGGTACCATCTTGGCCGTGCTAACAAACCAGGTCATTAAAACAGCTAATGCTGCAAAAGATGTTTTCGGCAAGGAGAGTTTCAAGGTCCTTTCGAAGCACTTGCTTGACATTGGCTCGGTCTTGAACGAACTACAGCGTCAAGAATTGAATGACTCTCAAGCTGCCAGGCTTGCTTTGGAGTCCCTCGAAGCAGATGTTAAAAGGGCAAATAATCTGGTTGACAAGTATAAAAACCGAGCACGCTTCTACTTGCTTGTCAAGTGCCGGCACATTGTCAAGGAGGTACAAGATGTTACCAGGGATATTGGAAGGTCTTTGGCTGCTCTGTCCGTTGCAAACACCGACGTCTTATTGGGGATATCTGATCAGGTGGATAGGTTACAGAATGAAATGCAGAGAGTGGAAATTGAGGCCTCACAGTCTCAGCTCCAAGTTTTCGACAAGCTAAAACAAGGTCTTGATGATCAGACACTTGACCAAGGTTTTGCAAACGATATGCTTGCAGAAATAGCGATGGCAGTTGGGGTGCCTTTAGAGCCCTCAGAGATAAGCAAAGAGCTTGCAGATTTCAGAAAGGAGAAAGAAGAAGCTGCCAACAGGAAAGAAAGAGCTGAAGTTTTCTTCTTAGATCAGATTATTGAGCTGCTTTCTCGAGCTGATGCTGCAAGGGATTATGAAGAAGTCAAGAAACAATACAAGCAACGTGTGCAGGCCATACAGAGATACGACTCGAGCGAAGAATATATTCCACCACTTAAGCCTTTTATATGCTGTATTAGAAAAACTGTGATGGCTGAGCCAGTCAGTCTTTGCACCGGTACTACATGTGAGAGAGCTGCAATCATAACTTGGTTTGAAAGTGGTGAGAGAACTGACCCTGAAACTCATGAGGTTCTTCAAGATACTTCCTGGAGGTGTAACATCCCACTGAGACAATCAATAGAAGAGTGGAGAGAGCTAAATTATTGTCTCAAGATTCGAACTGCCAAGGCAAAGTTGTTATCGGGCTTTGAGACGTCAATATTAGAGGCCTTAAGCAAAATGCAAGATCTTATGACAGAGAGTTCTATTAACAAGGACTGGCTCACCATTGAAGGGCTCACCGACATTATAATCTCTATACTTGGAAACTCTCACGACAGAGATTTGAAAAGGAGGATTTTGATCACCTTAAAGGATATTGTCGAAGGGCATGCAAGAAACAAGGAGAAAGTGGTTGAATCCCAGGGGTGGGATCACATCGTTCCCTGCTTAGGGCGCGACTCAAGCATCTCAAAGGCTGCAATTGAGTTGCTATATGAATTGTTGCAAGACAGAACTGGTTGGAATGTGTCTGTATGCAGGAAACTCTCTCAACAATGCAGTACAATTCTTTTCCTTGTTTACACCCTTTTAAAGGGTACTGTGAGGGTGTCAGCTGAGATTGCAGAGAAAATCTTGCTGAATCTTTTCGACATAGATGAGGAAAACATATCTCGTGCAGCTAAATCTGGCTGGTATAAGCCACTTGTTGATCGCATAGTTGAAGGGCCGGAAACTTCAAGGGTATCAATGGTGAGAACACTTGTTAACATGGAATTGGTAGACTCAAATTTGAAGCTCCTTGGTGAGCAAGGGATTATACCTCCTTTGCTCGAAATGGTGTCCGGAAATATTGAATCAAAACTATTGTCCTTATCCGCCTTGGCTGAATTATCAGGTTGCAGTGCCAACAAAGAACTGATTGCTGCTTCTGGTGGGGTAAGTCTTGTTCTAAAGCTAGCATTCACTCCGCATGTGCGTGCAATCATTGTTGTTAAATGTTATGAAATCCTGGAGAAATTTACATCTGACACTGATGGGGCCAAATTCTTTGTCGATGAAAACGGGTGCCAACTAGAGTTAGGGCCAATTGTCACCAATTTGACTGCATTACAACAGAATCATAACTTGGTCTACAATGTCCGAAGGCCTGCCTTGCGCTCACTTCTTGGAATCTGCAAGTTTGATGCGGAGCTGGTTAAAAAGGCTGTTCTCACTCCTAATGCCATGTCTCTAGTCCTTCCTCTCCTTGATGATTCTGACTCGGAGATTCGGGAGATTGCCATAAATTTGCTTTTCCTCTTCTCGCAGCACGAGCCTGAGGGGGTTGTGGAATACCTTCTCAGGCCAAGAAGGCTGGAGGCTTTAGTGGGATTCCTTGAGAATGAAGACAAGGATGATGCACAGATGGCTGCTGCTGGATTATTGGCGAACCTACCAAAATCGGAAAGATCACTAACTATGAAGTTAATCGAACTGGACGGACATACTGCGCTCATAAGCATTTTAAGAACAGGGACTCTGAAGGCAAAAGAAAATGCTCTAGGTGCTCTTTTCAGGTTTACGGATCCCACAAACCTGGAATCGCAGCGTACACTGGTTGAAGGAGGAGCATACCCTTTGCTTGTAAACTTTCTCAGGTCCAGCTCAGTGACTGCAAGGGCAAGAGCTGCAGCTCTGATTGGTAATCTTTCCACAAGCACTCCAAATCTCACCATTGTCTCCAAACCCTCCGGTTGCTGGTCCTGTTTCAAGCCATCGAATGCTCCACTATGCTCAGCGCACGGTGGTATCTGCAGTGTGACTTCCACATTCTGTCTTTTGGAAGCAAAAGCTTTGCCTGACTTAGTAAAACTGTTATCTGGAGAAGTGTATGAAACTGCCATCGAAGCAATTCAAACTCTTTCGACATTGGTTGTCGAAGCCTCTCCTCAAAGAGGAGCCAGCGTCTTGCATGAGGCTGATGCCATAAAGCCGATGTTGGAAATCTTGTCTTGGGGAACAGATTCTTTACAAGAAGATACGTTGAGTCTCCTCGAAAAGGTTTTCATGTCGAAGGAAATGGTTGATGTGTATGGATCAGCAGCTAGATTAAGTCTTGCTGGTCTAACTAGCACGAACATCCATGAGGATGGTGGCCATAGGAGGAAGGCTGCCAGGGTTTTGTCCCTCCTTGAACGTTATTCGAAATCAACTTCTTTTACTCCGGGACTGATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

U-box

Protein Analysis

1015

Amino Acids

112.33

Weight (kDa)

5.47

Isoelectric Point (pI)

38.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCAfunc	PF19584	19 - 145	7.1e-07	MCAfunc domain
U-box	PF04564	263 - 327	7.5e-11	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011550)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g01981
malus_domestica	MD13G1203200.v1.1 MD16G1204600.v1.1
prunus_persica	Prupe.1G025700_v2.0.a1 Prupe.1G025700_v2.0.a1 Prupe.1G025700_v2.0.a1
pyrus_communis	pycom13g17650 pycom16g17270
rosa_chinensis	RchiOBHm_Chr4g0388951
rosa_laevigata	RLG00000010004
rosa_multiflora	Rmu_sc0001395.1_g000012
rosa_roxburghii	Rroxscaffold_5G00335270
rosa_rugosa	Rorug03G0321600
rosa_samantha	Rh4AG026100 Rh4BG019300 Rh4CG029100 Rh4DG019800
rosa_wichuraiana	Rw0G003670 Rw4G001880 Rw4G001920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1136
AatII	GACGTC	2 cut(s)	372, 1043
Acc65I	GGTACC	1 cut(s)	26
AccB1I	GGYRCC	3 cut(s)	26, 550, 1893
AccI	GTMKAC	2 cut(s)	1608, 1959
AccII	CGCG	1 cut(s)	1284
AccIII	TCCGGA	1 cut(s)	1670
AciI	CCGC	3 cut(s)	1704, 1796, 2015
AclI	AACGTT	1 cut(s)	3004
AclWI	GGATC	4 cut(s)	1266, 2387, 2400, 2911
AcoI	YGGCCR	2 cut(s)	36, 2962
AcsI	RAATTY	4 cut(s)	1615, 1844, 1871, 2113
AcuI	CTGAAG	2 cut(s)	657, 2369
AcyI	GRCGYC	2 cut(s)	369, 1040
AfaI	GTAC	6 cut(s)	28, 309, 844, 1390, 1426, 2419
AfiI	CCNNNNNNNGG	9 cut(s)	1119, 1254, 1255, 1277, 1278, 1420, 2565, 2762, 2866
AflII	CTTAAG	2 cut(s)	779, 1057
AflIII	ACRYGT	2 cut(s)	727, 848
AgeI	ACCGGT	1 cut(s)	839
AjnI	CCWGG	8 cut(s)	51, 188, 320, 932, 1248, 1836, 2403, 2981
AjuI	GAANNNNNNNTTGG	2 cut(s)	1937, 1969
Alw21I	GWGCWC	2 cut(s)	265, 2377
Alw26I	GTCTC	7 cut(s)	450, 949, 998, 1031, 2061, 2551, 2861
AlwI	GGATC	4 cut(s)	1266, 2387, 2400, 2911
AlwNI	CAGNNNCTG	1 cut(s)	2480
Ama87I	CYCGRG	2 cut(s)	675, 754
Aor13HI	TCCGGA	1 cut(s)	1670
ApoI	RAATTY	4 cut(s)	1615, 1844, 1871, 2113
ArsI	GACNNNNNNTTYG	6 cut(s)	491, 523, 1679, 1711, 1863, 1895
AsiGI	ACCGGT	1 cut(s)	839
Asp700I	GAANNNNTTC	5 cut(s)	768, 1472, 2163, 2718, 2830
Asp718I	GGTACC	1 cut(s)	26
AspLEI	GCGC	4 cut(s)	1284, 1986, 2320, 2608
AspS9I	GGNCC	6 cut(s)	109, 1553, 1866, 1910, 2466, 2568
AsuC2I	CCSGG	1 cut(s)	3033
AsuHPI	GGTGA	8 cut(s)	899, 1102, 1117, 1183, 1591, 1643, 1912, 2530
AsuII	TTCGAA	3 cut(s)	116, 1003, 3011
AsuNHI	GCTAGC	1 cut(s)	1783
AvaI	CYCGRG	2 cut(s)	675, 754
AvaII	GGWCC	3 cut(s)	109, 2466, 2568
AxyI	CCTNAGG	1 cut(s)	2145
BaeI	ACNNNNGTAYC	2 cut(s)	2599, 2632
BalI	TGGCCA	1 cut(s)	2964
BamHI	GGATCC	1 cut(s)	2392
BanI	GGYRCC	3 cut(s)	26, 550, 1893
BanII	GRGCYC	2 cut(s)	564, 1125
BauI	CACGAG	2 cut(s)	1500, 2138
BbsI	GAAGAC	1 cut(s)	2220
Bbv12I	GWGCWC	2 cut(s)	265, 2377
BccI	CCATC	8 cut(s)	38, 532, 808, 1856, 2227, 2590, 2717, 2951
BceAI	ACGGC	1 cut(s)	23
BcgI	CGANNNNNNTGC	6 cut(s)	106, 140, 506, 540, 1199, 1233
BciT130I	CCWGG	8 cut(s)	53, 190, 322, 934, 1250, 1838, 2405, 2983
BclI	TGATCA	3 cut(s)	388, 487, 1188
BcnI	CCSGG	1 cut(s)	3033
BcoDI	GTCTC	7 cut(s)	450, 949, 998, 1031, 2061, 2551, 2861
BfaI	CTAG	6 cut(s)	1784, 1901, 2060, 2369, 2913, 2937
BfmI	CTRYAG	3 cut(s)	155, 2496, 2620
BfrI	CTTAAG	2 cut(s)	779, 1057
BglI	GCCNNNNNGGC	1 cut(s)	2179
BglII	AGATCT	1 cut(s)	1072
BlpI	GCTNAGC	2 cut(s)	819, 2602
Bme1390I	CCNGG	9 cut(s)	53, 190, 322, 934, 1250, 1838, 2405, 2983, 3033
Bme18I	GGWCC	3 cut(s)	109, 2466, 2568
BmeT110I	CYCGRG	2 cut(s)	675, 754
BmgT120I	GGNCC	6 cut(s)	109, 1553, 1866, 1910, 2466, 2568
BmiI	GGNNCC	6 cut(s)	28, 552, 1867, 1895, 2394, 2767
BmrFI	CCNGG	9 cut(s)	53, 190, 322, 934, 1250, 1838, 2405, 2983, 3033
BmsI	GCATC	5 cut(s)	673, 1302, 2002, 2215, 2779
BmtI	GCTAGC	1 cut(s)	1787
BpiI	GAAGAC	1 cut(s)	2220
BpmI	CTGGAG	4 cut(s)	955, 1859, 2204, 2709
Bpu10I	CCTNAGC	1 cut(s)	1276
Bpu1102I	GCTNAGC	2 cut(s)	819, 2602
Bpu14I	TTCGAA	3 cut(s)	116, 1003, 3011
BpuEI	CTTGAG	4 cut(s)	165, 980, 1273, 2225
BpuMI	CCSGG	1 cut(s)	3033
BsaBI	GATNNNNATC	1 cut(s)	1187
BsaHI	GRCGYC	2 cut(s)	369, 1040
BsaJI	CCNNGG	8 cut(s)	321, 502, 1011, 1248, 1249, 1627, 1707, 2982
BsaWI	WCCGGW	3 cut(s)	839, 1670, 2558
BsaXI	ACNNNNNCTCC	4 cut(s)	2512, 2542, 2757, 2787
Bsc4I	CCNNNNNNNGG	9 cut(s)	1119, 1254, 1255, 1277, 1278, 1420, 2565, 2762, 2866
Bse118I	RCCGGY	2 cut(s)	288, 839
Bse1I	ACTGG	5 cut(s)	824, 1109, 1348, 2307, 2427
Bse21I	CCTNAGG	1 cut(s)	2145
Bse8I	GATNNNNATC	1 cut(s)	1187
BseAI	TCCGGA	1 cut(s)	1670
BseBI	CCWGG	8 cut(s)	53, 190, 322, 934, 1250, 1838, 2405, 2983
BseDI	CCNNGG	8 cut(s)	321, 502, 1011, 1248, 1249, 1627, 1707, 2982
BseGI	GGATG	4 cut(s)	945, 2227, 2946, 2962
BseJI	GATNNNNATC	1 cut(s)	1187
BseLI	CCNNNNNNNGG	9 cut(s)	1119, 1254, 1255, 1277, 1278, 1420, 2565, 2762, 2866
BseNI	ACTGG	5 cut(s)	824, 1109, 1348, 2307, 2427
BseRI	GAGGAG	4 cut(s)	2448, 2745, 2778, 2849
BsgI	GTGCAG	2 cut(s)	752, 1524
Bsh1236I	CGCG	1 cut(s)	1284
BshNI	GGYRCC	3 cut(s)	26, 550, 1893
BshTI	ACCGGT	1 cut(s)	839
BsiHKAI	GWGCWC	2 cut(s)	265, 2377
BsiHKCI	CYCGRG	2 cut(s)	675, 754
BsiSI	CCGG	6 cut(s)	289, 840, 1556, 1671, 2559, 3032
BslFI	GGGAC	3 cut(s)	190, 2356, 2977
BslI	CCNNNNNNNGG	9 cut(s)	1119, 1254, 1255, 1277, 1278, 1420, 2565, 2762, 2866
BsmAI	GTCTC	7 cut(s)	450, 949, 998, 1031, 2061, 2551, 2861
BsmBI	CGTCTC	1 cut(s)	1031
BsmFI	GGGAC	3 cut(s)	190, 2356, 2977
BsmI	GAATGC	2 cut(s)	1787, 2593
BsoBI	CYCGRG	2 cut(s)	675, 754
Bsp119I	TTCGAA	3 cut(s)	116, 1003, 3011
Bsp1286I	GDGCHC	4 cut(s)	265, 564, 1125, 2377
Bsp13I	TCCGGA	1 cut(s)	1670
Bsp1720I	GCTNAGC	2 cut(s)	819, 2602
BspACI	CCGC	3 cut(s)	1704, 1796, 2015
BspEI	TCCGGA	1 cut(s)	1670
BspFNI	CGCG	1 cut(s)	1284
BspHI	TCATGA	1 cut(s)	910
BspLI	GGNNCC	6 cut(s)	28, 552, 1867, 1895, 2394, 2767
BspMAI	CTGCAG	2 cut(s)	2500, 2624
BspOI	GCTAGC	1 cut(s)	1787
BspPI	GGATC	4 cut(s)	1266, 2387, 2400, 2911
BspT104I	TTCGAA	3 cut(s)	116, 1003, 3011
BspT107I	GGYRCC	3 cut(s)	26, 550, 1893
BspTI	CTTAAG	2 cut(s)	779, 1057
BsrFI	RCCGGY	2 cut(s)	288, 839
BsrI	ACTGG	5 cut(s)	824, 1109, 1348, 2307, 2427
BssAI	RCCGGY	2 cut(s)	288, 839
BssECI	CCNNGG	8 cut(s)	321, 502, 1011, 1248, 1249, 1627, 1707, 2982
BssNI	GRCGYC	2 cut(s)	369, 1040
BssSI	CACGAG	2 cut(s)	1500, 2138
BssT1I	CCWWGG	4 cut(s)	502, 1011, 1627, 1707
Bst2BI	CACGAG	2 cut(s)	1500, 2138
Bst2UI	CCWGG	8 cut(s)	53, 190, 322, 934, 1250, 1838, 2405, 2983
Bst4CI	ACNGT	5 cut(s)	444, 811, 1429, 2612, 2682
Bst6I	CTCTTC	2 cut(s)	963, 2132
BstACI	GRCGYC	2 cut(s)	369, 1040
BstAFI	CTTAAG	2 cut(s)	779, 1057
BstAPI	GCANNNNNTGC	2 cut(s)	730, 2495
BstBI	TTCGAA	3 cut(s)	116, 1003, 3011
BstENI	CCTNNNNNAGG	3 cut(s)	1418, 2760, 2864
BstF5I	GGATG	4 cut(s)	945, 2227, 2946, 2962
BstFNI	CGCG	1 cut(s)	1284
BstHHI	GCGC	4 cut(s)	1284, 1986, 2320, 2608
BstMAI	GTCTC	7 cut(s)	450, 949, 998, 1031, 2061, 2551, 2861
BstNI	CCWGG	8 cut(s)	53, 190, 322, 934, 1250, 1838, 2405, 2983
BstNSI	RCATGY	3 cut(s)	852, 1220, 1802
BstSCI	CCNGG	9 cut(s)	51, 188, 320, 932, 1248, 1836, 2403, 2981, 3031
BstSFI	CTRYAG	3 cut(s)	155, 2496, 2620
BstUI	CGCG	1 cut(s)	1284
BstV2I	GAAGAC	1 cut(s)	2220
BstX2I	RGATCY	2 cut(s)	1072, 2392
BstXI	CCANNNNNNTGG	2 cut(s)	2404, 2957
BstYI	RGATCY	2 cut(s)	1072, 2392
Bsu36I	CCTNAGG	1 cut(s)	2145
BtgZI	GCGATG	1 cut(s)	551
BtsCI	GGATG	4 cut(s)	945, 2227, 2946, 2962
BtsI	GCAGTG	3 cut(s)	12, 1735, 2629
BtsIMutI	CAGTG	7 cut(s)	12, 950, 1735, 1857, 2420, 2481, 2629
CaiI	CAGNNNCTG	1 cut(s)	2480
CciI	TCATGA	1 cut(s)	910
CfoI	GCGC	4 cut(s)	1284, 1986, 2320, 2608
Cfr10I	RCCGGY	2 cut(s)	288, 839
Cfr13I	GGNCC	6 cut(s)	109, 1553, 1866, 1910, 2466, 2568
CseI	GACGC	2 cut(s)	149, 2761
CsiI	ACCWGGT	1 cut(s)	51
Csp6I	GTAC	6 cut(s)	27, 308, 843, 1389, 1425, 2418
CspAI	ACCGGT	1 cut(s)	839
CviAII	CATG	9 cut(s)	849, 911, 1217, 1597, 1799, 2053, 2780, 2875, 2951
CviQI	GTAC	6 cut(s)	27, 308, 843, 1389, 1425, 2418
DraI	TTTAAA	1 cut(s)	1419
EaeI	YGGCCR	2 cut(s)	36, 2962
Eam1104I	CTCTTC	2 cut(s)	963, 2132
EarI	CTCTTC	2 cut(s)	963, 2132
EciI	GGCGGA	1 cut(s)	1693
Eco130I	CCWWGG	4 cut(s)	502, 1011, 1627, 1707
Eco147I	AGGCCT	3 cut(s)	437, 1055, 1975
Eco24I	GRGCYC	2 cut(s)	564, 1125
Eco32I	GATATC	1 cut(s)	384
Eco47I	GGWCC	3 cut(s)	109, 2466, 2568
Eco57I	CTGAAG	2 cut(s)	657, 2369
Eco81I	CCTNAGG	1 cut(s)	2145
Eco88I	CYCGRG	2 cut(s)	675, 754
EcoNI	CCTNNNNNAGG	3 cut(s)	1418, 2760, 2864
EcoO109I	RGGNCCY	1 cut(s)	109
EcoRII	CCWGG	8 cut(s)	51, 188, 320, 932, 1248, 1836, 2403, 2981
EcoRV	GATATC	1 cut(s)	384
EcoT14I	CCWWGG	4 cut(s)	502, 1011, 1627, 1707
EcoT38I	GRGCYC	2 cut(s)	564, 1125
ErhI	CCWWGG	4 cut(s)	502, 1011, 1627, 1707
Esp3I	CGTCTC	1 cut(s)	1031
FaeI	CATG	9 cut(s)	852, 914, 1220, 1600, 1802, 2056, 2783, 2878, 2954
FaqI	GGGAC	3 cut(s)	190, 2356, 2977
FatI	CATG	9 cut(s)	848, 910, 1216, 1596, 1798, 2052, 2779, 2874, 2950
FbaI	TGATCA	3 cut(s)	388, 487, 1188
FblI	GTMKAC	2 cut(s)	1608, 1959
FokI	GGATG	4 cut(s)	932, 2234, 2933, 2969
FriOI	GRGCYC	2 cut(s)	564, 1125
FspBI	CTAG	6 cut(s)	1784, 1901, 2060, 2369, 2913, 2937
GlaI	GCGC	4 cut(s)	1283, 1985, 2319, 2607
GsuI	CTGGAG	4 cut(s)	955, 1859, 2204, 2709
HapII	CCGG	6 cut(s)	289, 840, 1556, 1671, 2559, 3032
HgaI	GACGC	2 cut(s)	149, 2761
HhaI	GCGC	4 cut(s)	1284, 1986, 2320, 2608
Hin1I	GRCGYC	2 cut(s)	369, 1040
Hin1II	CATG	9 cut(s)	852, 914, 1220, 1600, 1802, 2056, 2783, 2878, 2954
Hin6I	GCGC	4 cut(s)	1282, 1984, 2318, 2606
HinP1I	GCGC	4 cut(s)	1282, 1984, 2318, 2606
HincII	GTYRAC	2 cut(s)	244, 1594
HindII	GTYRAC	2 cut(s)	244, 1594
HindIII	AAGCTT	1 cut(s)	2658
HpaI	GTTAAC	1 cut(s)	1594
HpaII	CCGG	6 cut(s)	289, 840, 1556, 1671, 2559, 3032
HphI	GGTGA	8 cut(s)	899, 1102, 1117, 1183, 1591, 1643, 1912, 2530
Hpy166II	GTNNAC	8 cut(s)	244, 1408, 1594, 1609, 1960, 2388, 2420, 2455
Hpy8I	GTNNAC	8 cut(s)	244, 1408, 1594, 1609, 1960, 2388, 2420, 2455
Hpy99I	CGWCG	1 cut(s)	371
HpyCH4III	ACNGT	5 cut(s)	444, 811, 1429, 2612, 2682
HpyCH4IV	ACGT	5 cut(s)	369, 727, 1040, 2849, 3004
HpySE526I	ACGT	5 cut(s)	369, 727, 1040, 2849, 3004
Hsp92I	GRCGYC	2 cut(s)	369, 1040
Hsp92II	CATG	9 cut(s)	852, 914, 1220, 1600, 1802, 2056, 2783, 2878, 2954
HspAI	GCGC	4 cut(s)	1282, 1984, 2318, 2606
Kpn2I	TCCGGA	1 cut(s)	1670
KpnI	GGTACC	1 cut(s)	30
KroI	GCCGGC	1 cut(s)	288
KroNI	GCCGGC	1 cut(s)	290
Ksp22I	TGATCA	3 cut(s)	388, 487, 1188
KspAI	GTTAAC	1 cut(s)	1594
LmnI	GCTCC	7 cut(s)	10, 456, 1629, 2017, 2435, 2596, 2765
LweI	GCATC	5 cut(s)	673, 1302, 2002, 2215, 2779
MabI	ACCWGGT	1 cut(s)	51
MaeI	CTAG	6 cut(s)	1784, 1901, 2060, 2369, 2913, 2937
MaeII	ACGT	5 cut(s)	369, 727, 1040, 2849, 3004
MaeIII	GTNAC	6 cut(s)	316, 402, 941, 1918, 2476, 2626
MboII	GAAGA	9 cut(s)	620, 637, 713, 773, 911, 980, 2119, 2225, 2855
MfeI	CAATTG	2 cut(s)	1308, 1914
MflI	RGATCY	2 cut(s)	1072, 2392
MhlI	GDGCHC	4 cut(s)	265, 564, 1125, 2377
MlsI	TGGCCA	1 cut(s)	2964
MluNI	TGGCCA	1 cut(s)	2964
MlyI	GAGTC	8 cut(s)	170, 212, 746, 1280, 1604, 2081, 2338, 2863
MmeI	TCCRAC	2 cut(s)	1328, 2787
Mox20I	TGGCCA	1 cut(s)	2964
MroI	TCCGGA	1 cut(s)	1670
MroNI	GCCGGC	1 cut(s)	288
MroXI	GAANNNNTTC	5 cut(s)	768, 1472, 2163, 2718, 2830
MscI	TGGCCA	1 cut(s)	2964
MslI	CAYNNNNRTG	2 cut(s)	1803, 2955
Msp20I	TGGCCA	1 cut(s)	2964
MspA1I	CMGCKG	1 cut(s)	1442
MspCI	CTTAAG	2 cut(s)	779, 1057
MspI	CCGG	6 cut(s)	289, 840, 1556, 1671, 2559, 3032
MspR9I	CCNGG	9 cut(s)	53, 190, 322, 934, 1250, 1838, 2405, 2983, 3033
MunI	CAATTG	2 cut(s)	1308, 1914
Mva1269I	GAATGC	2 cut(s)	1787, 2593
MvaI	CCWGG	8 cut(s)	53, 190, 322, 934, 1250, 1838, 2405, 2983
MvnI	CGCG	1 cut(s)	1284
NaeI	GCCGGC	1 cut(s)	290
NciI	CCSGG	1 cut(s)	3033
NgoMIV	GCCGGC	1 cut(s)	288
NheI	GCTAGC	1 cut(s)	1783
NlaIII	CATG	9 cut(s)	852, 914, 1220, 1600, 1802, 2056, 2783, 2878, 2954
NlaIV	GGNNCC	6 cut(s)	28, 552, 1867, 1895, 2394, 2767
NmuCI	GTSAC	3 cut(s)	1918, 2476, 2626
NspI	RCATGY	3 cut(s)	852, 1220, 1802
NspV	TTCGAA	3 cut(s)	116, 1003, 3011
PaeI	GCATGC	1 cut(s)	1220
PaeR7I	CTCGAG	2 cut(s)	675, 754
PagI	TCATGA	1 cut(s)	910
PasI	CCCWGGG	1 cut(s)	1249
PceI	AGGCCT	3 cut(s)	437, 1055, 1975
PciI	ACATGT	1 cut(s)	848
PcsI	WCGNNNNNNNCGW	1 cut(s)	756
PctI	GAATGC	2 cut(s)	1787, 2593
PdiI	GCCGGC	1 cut(s)	290
PdmI	GAANNNNTTC	5 cut(s)	768, 1472, 2163, 2718, 2830
PfoI	TCCNGGA	3 cut(s)	932, 1836, 3031
PinAI	ACCGGT	1 cut(s)	839
PleI	GAGTC	8 cut(s)	170, 211, 746, 1280, 1604, 2081, 2338, 2862
PpsI	GAGTC	8 cut(s)	170, 211, 746, 1280, 1604, 2081, 2338, 2862
PpuMI	RGGWCCY	1 cut(s)	109
PscI	ACATGT	1 cut(s)	848
PsiI	TTATAA	1 cut(s)	1136
Psp1406I	AACGTT	1 cut(s)	3004
Psp5II	RGGWCCY	1 cut(s)	109
Psp6I	CCWGG	8 cut(s)	51, 188, 320, 932, 1248, 1836, 2403, 2981
PspGI	CCWGG	8 cut(s)	51, 188, 320, 932, 1248, 1836, 2403, 2981
PspN4I	GGNNCC	6 cut(s)	28, 552, 1867, 1895, 2394, 2767
PspPI	GGNCC	6 cut(s)	109, 1553, 1866, 1910, 2466, 2568
PspPPI	RGGWCCY	1 cut(s)	109
PspXI	VCTCGAGB	1 cut(s)	754
PsrI	GAACNNNNNNTAC	2 cut(s)	1758, 1790
PstI	CTGCAG	2 cut(s)	2500, 2624
PstNI	CAGNNNCTG	1 cut(s)	2480
PsuI	RGATCY	2 cut(s)	1072, 2392
PvuII	CAGCTG	1 cut(s)	1442
RsaI	GTAC	6 cut(s)	28, 309, 844, 1390, 1426, 2419
RsaNI	GTAC	6 cut(s)	27, 308, 843, 1389, 1425, 2418
RseI	CAYNNNNRTG	2 cut(s)	1803, 2955
Sau96I	GGNCC	6 cut(s)	109, 1553, 1866, 1910, 2466, 2568
SchI	GAGTC	8 cut(s)	170, 212, 746, 1280, 1604, 2081, 2338, 2863
ScrFI	CCNGG	9 cut(s)	53, 190, 322, 934, 1250, 1838, 2405, 2983, 3033
SduI	GDGCHC	4 cut(s)	265, 564, 1125, 2377
SexAI	ACCWGGT	1 cut(s)	51
SfaNI	GCATC	5 cut(s)	673, 1302, 2002, 2215, 2779
SfcI	CTRYAG	3 cut(s)	155, 2496, 2620
Sfr274I	CTCGAG	2 cut(s)	675, 754
SfuI	TTCGAA	3 cut(s)	116, 1003, 3011
SinI	GGWCC	3 cut(s)	109, 2466, 2568
SlaI	CTCGAG	2 cut(s)	675, 754
SmiMI	CAYNNNNRTG	2 cut(s)	1803, 2955
SmlI	CTYRAG	8 cut(s)	180, 675, 754, 779, 995, 1057, 1288, 2204
SmoI	CTYRAG	8 cut(s)	180, 675, 754, 779, 995, 1057, 1288, 2204
SphI	GCATGC	1 cut(s)	1220
SseBI	AGGCCT	3 cut(s)	437, 1055, 1975
SsiI	CCGC	3 cut(s)	1704, 1796, 2015
SspI	AATATT	2 cut(s)	1047, 1678
SspMI	CTAG	6 cut(s)	1784, 1901, 2060, 2369, 2913, 2937
StuI	AGGCCT	3 cut(s)	437, 1055, 1975
StyD4I	CCNGG	9 cut(s)	51, 188, 320, 932, 1248, 1836, 2403, 2981, 3031
StyI	CCWWGG	4 cut(s)	502, 1011, 1627, 1707
TaaI	ACNGT	5 cut(s)	444, 811, 1429, 2612, 2682
TaiI	ACGT	5 cut(s)	372, 730, 1043, 2852, 3007
TaqII	GACCGA	1 cut(s)	130
TatI	WGTACW	1 cut(s)	1388
TscAI	CASTG	7 cut(s)	12, 957, 1735, 1864, 2427, 2481, 2629
TseFI	GTSAC	3 cut(s)	1918, 2476, 2626
Tsp45I	GTSAC	3 cut(s)	1918, 2476, 2626
TspDTI	ATGAA	9 cut(s)	426, 714, 1338, 1845, 1899, 2226, 2303, 2715, 2863
TspGWI	ACGGA	3 cut(s)	343, 2322, 2405
TspRI	CASTG	7 cut(s)	12, 957, 1735, 1864, 2427, 2481, 2629
Vha464I	CTTAAG	2 cut(s)	779, 1057
VpaK11BI	GGWCC	3 cut(s)	109, 2466, 2568
XagI	CCTNNNNNAGG	3 cut(s)	1418, 2760, 2864
XapI	RAATTY	4 cut(s)	1615, 1844, 1871, 2113
XceI	RCATGY	3 cut(s)	852, 1220, 1802
XcmI	CCANNNNNNNNNTGG	2 cut(s)	196, 2957
XhoI	CTCGAG	2 cut(s)	675, 754
XmiI	GTMKAC	2 cut(s)	1608, 1959
XmnI	GAANNNNTTC	5 cut(s)	768, 1472, 2163, 2718, 2830
XspI	CTAG	6 cut(s)	1784, 1901, 2060, 2369, 2913, 2937
ZraI	GACGTC	2 cut(s)	370, 1041

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.