MD16G1230400.v1.1

HAD-hyrolase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016053
Physical Location & Seq
Reverse (-)
23613063 .. 23616206
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1230400.v1.1.491

Sequence Viewer

Length: 756 bp
ATGCCACTACTACTACTGCCCAGAACACTCCTCTTCCTGTACCCAAAACTCAAAATTACCAAAAGACCCATGGCCACAATGGGTACCACCACCGCCCAACCACCCAGGGTGCGCCTCCGAGGCGTTGTCTTCGACATGGACGGAACCCTAACGGTCCCCGTCATCGATTTCGCCGCAATGTACAGAGCCGTGCTCGGCGAAGACGAGTACGTCAGAATCAAGGCTGAGAACCCTTCCGGGATCGATATATTGCACCACATTGAGAATTGGAGCCCCGAGAAGCAGCGGAAGGCGTATGATATTATCGCCGATTTTGAGCGCCAGGGGAGCGATCGCCTTCAGATCATGCCTGGTGCAGCAGAACTTTGTGGTTTTCTAGATTCTAAGAATATAAGGAGGGGATTAATCACACGTAATGTCAAGGCTGCTGTAGATTTGTTTCATGATCGGTTCAGTTTGTCATTTTCTCCAGCGTTGAGCAGGGAATTTCGACCTTACAAACCGGATCCATCTCCACTACTGCATATATGTTCAGCTTGGGAAGTTAAACCAAGTGAAGTAATGATGGTTGGAGACTCCCTCAAAGATGATGTGGCTTGTGGGAAGCAGGCAGGAGCTGTTGCGTGCTTGCTCGATGAAAATGGAAGGTATGACTCTCCAGAATATGCCAATGTCGAATTCAAACCAGATTTCAAGGTGACTTCCCTAAGGGAAGTTCACACGCTATTGGAAGCACATTTTGACCTGTCACCCTGA

Protein Analysis

252

Amino Acids

28.28

Weight (kDa)

6.32

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 40 - 205 2.5e-19 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 42 - 206 2.5e-13 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 167 - 238 2.3e-10 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33255
fragaria_vesca FvH4_4g05140
malus_domestica MD16G1230400.v1.1
prunus_persica Prupe.1G052300_v2.0.a1
pyrus_communis pycom16g19220
rosa_chinensis RchiOBHm_Chr4g0396071
rosa_laevigata RLG00000009575
rosa_multiflora Rmu_sc0006325.1_g000009 Rmu_sc0015594.1_g000003
rosa_roxburghii Rroxscaffold_5G00340870
rosa_rugosa Rorug03G0352800
rosa_samantha Rh4AG065000 Rh4AG065700 Rh4CG070400
rosa_wichuraiana Rw4G005290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
Acc65I GGTACC 1 cut(s) 83
AccB1I GGYRCC 1 cut(s) 83
AciI CCGC 3 cut(s) 93, 174, 286
AclWI GGATC 3 cut(s) 248, 500, 513
AcoI YGGCCR 1 cut(s) 72
AcsI RAATTY 2 cut(s) 485, 677
AcuI CTGAAG 1 cut(s) 323
AfaI GTAC 4 cut(s) 41, 85, 182, 209
AflIII ACRYGT 1 cut(s) 410
AgsI TTSAA 2 cut(s) 682, 694
AjnI CCWGG 3 cut(s) 104, 321, 349
AjuI GAANNNNNNNTTGG 2 cut(s) 662, 694
AluBI AGCT 2 cut(s) 536, 617
AluI AGCT 2 cut(s) 536, 617
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 567
AlwI GGATC 3 cut(s) 248, 500, 513
AlwNI CAGNNNCTG 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 275
AoxI GGCC 1 cut(s) 72
ApeKI GCWGC 3 cut(s) 283, 356, 425
ApoI RAATTY 2 cut(s) 485, 677
AseI ATTAAT 1 cut(s) 404
AsiSI GCGATCGC 1 cut(s) 334
Asp718I GGTACC 1 cut(s) 83
AspLEI GCGC 2 cut(s) 114, 321
AspS9I GGNCC 1 cut(s) 154
AsuC2I CCSGG 1 cut(s) 238
AsuHPI GGTGA 2 cut(s) 709, 741
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 1 cut(s) 154
AxyI CCTNAGG 1 cut(s) 707
BalI TGGCCA 1 cut(s) 74
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 1 cut(s) 275
BbsI GAAGAC 2 cut(s) 121, 207
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 3 cut(s) 295, 368, 412
BccI CCATC 2 cut(s) 517, 559
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 3 cut(s) 106, 323, 351
BcnI CCSGG 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 567
BfaI CTAG 1 cut(s) 377
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 322
BglI GCCNNNNNGGC 1 cut(s) 120
BisI GCNGC 4 cut(s) 174, 284, 357, 426
BlsI GCNGC 4 cut(s) 175, 285, 358, 427
Bme1390I CCNGG 4 cut(s) 106, 238, 323, 351
Bme18I GGWCC 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 154
BmiI GGNNCC 5 cut(s) 85, 145, 156, 272, 507
BmrFI CCNGG 4 cut(s) 106, 238, 323, 351
BpiI GAAGAC 2 cut(s) 121, 207
BplI GAGNNNNNCTC 4 cut(s) 177, 209, 564, 596
BpmI CTGGAG 2 cut(s) 453, 642
BpuMI CCSGG 1 cut(s) 238
Bsa29I ATCGAT 2 cut(s) 165, 243
BsaAI YACGTR 1 cut(s) 413
BsaJI CCNNGG 5 cut(s) 69, 104, 105, 118, 322
BsaWI WCCGGW 1 cut(s) 502
Bse21I CCTNAGG 1 cut(s) 707
Bse3DI GCAATG 1 cut(s) 183
BseBI CCWGG 3 cut(s) 106, 323, 351
BseCI ATCGAT 2 cut(s) 165, 243
BseDI CCNNGG 5 cut(s) 69, 104, 105, 118, 322
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 3 cut(s) 295, 368, 412
BsgI GTGCAG 1 cut(s) 375
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 1 cut(s) 74
BshNI GGYRCC 1 cut(s) 83
BshVI ATCGAT 2 cut(s) 165, 243
BsiEI CGRYCG 1 cut(s) 334
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 2 cut(s) 237, 503
BslFI GGGAC 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 567
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 195, 275
Bsp1407I TGTACA 1 cut(s) 180
Bsp143I GATC 5 cut(s) 240, 331, 342, 445, 505
Bsp19I CCATGG 1 cut(s) 69
BspACI CCGC 3 cut(s) 93, 174, 286
BspANI GGCC 1 cut(s) 74
BspCNI CTCAG 1 cut(s) 217
BspDI ATCGAT 2 cut(s) 165, 243
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 5 cut(s) 85, 145, 156, 272, 507
BspPI GGATC 3 cut(s) 248, 500, 513
BspT107I GGYRCC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 183
BsrGI TGTACA 1 cut(s) 180
BssECI CCNNGG 5 cut(s) 69, 104, 105, 118, 322
BssMI GATC 5 cut(s) 240, 331, 342, 445, 505
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 3 cut(s) 106, 323, 351
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 1 cut(s) 38
BstAUI TGTACA 1 cut(s) 180
BstBAI YACGTR 1 cut(s) 413
BstC8I GCNNGC 3 cut(s) 609, 625, 629
BstDEI CTNAG 3 cut(s) 225, 384, 707
BstDSI CCRYGG 1 cut(s) 69
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 2 cut(s) 114, 321
BstKTI GATC 5 cut(s) 243, 334, 345, 448, 508
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 5 cut(s) 240, 331, 342, 445, 505
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 2 cut(s) 120, 327
BstNI CCWGG 3 cut(s) 106, 323, 351
BstSCI CCNGG 4 cut(s) 104, 236, 321, 349
BstSFI CTRYAG 1 cut(s) 429
BstV1I GCAGC 3 cut(s) 295, 368, 412
BstV2I GAAGAC 2 cut(s) 121, 207
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
Bsu15I ATCGAT 2 cut(s) 165, 243
Bsu36I CCTNAGG 1 cut(s) 707
BsuRI GGCC 1 cut(s) 74
BsuTUI ATCGAT 2 cut(s) 165, 243
BtgI CCRYGG 1 cut(s) 69
Cac8I GCNNGC 3 cut(s) 609, 625, 629
CaiI CAGNNNCTG 1 cut(s) 617
CciI TCATGA 1 cut(s) 442
CfoI GCGC 2 cut(s) 114, 321
Cfr13I GGNCC 1 cut(s) 154
ClaI ATCGAT 2 cut(s) 165, 243
Csp6I GTAC 4 cut(s) 40, 84, 181, 208
CviAII CATG 4 cut(s) 70, 136, 346, 443
CviJI RGCY 8 cut(s) 74, 188, 224, 273, 425, 536, 596, 617
CviKI_1 RGCY 8 cut(s) 74, 188, 224, 273, 425, 536, 596, 617
CviQI GTAC 4 cut(s) 40, 84, 181, 208
DdeI CTNAG 3 cut(s) 225, 384, 707
DpnI GATC 5 cut(s) 242, 333, 344, 447, 507
DpnII GATC 5 cut(s) 240, 331, 342, 445, 505
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 1 cut(s) 72
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 69
Eco24I GRGCYC 1 cut(s) 275
Eco47I GGWCC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 323
Eco81I CCTNAGG 1 cut(s) 707
Eco88I CYCGRG 1 cut(s) 275
EcoRI GAATTC 1 cut(s) 677
EcoRII CCWGG 3 cut(s) 104, 321, 349
EcoT14I CCWWGG 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 275
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 4 cut(s) 73, 139, 349, 446
FaqI GGGAC 1 cut(s) 140
FatI CATG 4 cut(s) 69, 135, 345, 442
Fnu4HI GCNGC 4 cut(s) 174, 284, 357, 426
FriOI GRGCYC 1 cut(s) 275
Fsp4HI GCNGC 4 cut(s) 174, 284, 357, 426
FspBI CTAG 1 cut(s) 377
GlaI GCGC 2 cut(s) 113, 320
GluI GCNGC 4 cut(s) 174, 284, 357, 426
GsuI CTGGAG 2 cut(s) 453, 642
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 1 cut(s) 74
HapII CCGG 2 cut(s) 237, 503
HhaI GCGC 2 cut(s) 114, 321
Hin1II CATG 4 cut(s) 73, 139, 349, 446
Hin6I GCGC 2 cut(s) 112, 319
HinP1I GCGC 2 cut(s) 112, 319
HinfI GANTC 4 cut(s) 216, 380, 575, 653
HpaII CCGG 2 cut(s) 237, 503
HphI GGTGA 2 cut(s) 709, 741
Hpy166II GTNNAC 1 cut(s) 718
Hpy188I TCNGA 3 cut(s) 119, 215, 342
Hpy188III TCNNGA 3 cut(s) 377, 443, 659
Hpy8I GTNNAC 1 cut(s) 718
HpyAV CCTTC 4 cut(s) 243, 283, 347, 639
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 2 cut(s) 210, 412
HpyCH4V TGCA 3 cut(s) 253, 356, 523
HpyF10VI GCNNNNNNNGC 2 cut(s) 120, 327
HpyF3I CTNAG 3 cut(s) 225, 384, 707
HpySE526I ACGT 2 cut(s) 210, 412
Hsp92II CATG 4 cut(s) 73, 139, 349, 446
HspAI GCGC 2 cut(s) 112, 319
KpnI GGTACC 1 cut(s) 87
Kzo9I GATC 5 cut(s) 240, 331, 342, 445, 505
LmnI GCTCC 3 cut(s) 270, 327, 614
Lsp1109I GCAGC 3 cut(s) 295, 368, 412
MaeI CTAG 1 cut(s) 377
MaeII ACGT 2 cut(s) 210, 412
MaeIII GTNAC 2 cut(s) 697, 747
MalI GATC 5 cut(s) 242, 333, 344, 447, 507
MboI GATC 5 cut(s) 240, 331, 342, 445, 505
MboII GAAGA 3 cut(s) 25, 121, 212
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 2 cut(s) 195, 275
MlsI TGGCCA 1 cut(s) 74
MluCI AATT 4 cut(s) 54, 265, 485, 677
MluNI TGGCCA 1 cut(s) 74
MlyI GAGTC 2 cut(s) 569, 647
MmeI TCCRAC 1 cut(s) 550
MnlI CCTC 5 cut(s) 41, 113, 125, 390, 590
Mox20I TGGCCA 1 cut(s) 74
MscI TGGCCA 1 cut(s) 74
MseI TTAA 2 cut(s) 404, 546
Msp20I TGGCCA 1 cut(s) 74
MspA1I CMGCKG 1 cut(s) 286
MspI CCGG 2 cut(s) 237, 503
MspR9I CCNGG 4 cut(s) 106, 238, 323, 351
MvaI CCWGG 3 cut(s) 106, 323, 351
MwoI GCNNNNNNNGC 2 cut(s) 120, 327
NciI CCSGG 1 cut(s) 238
NcoI CCATGG 1 cut(s) 69
NdeII GATC 5 cut(s) 240, 331, 342, 445, 505
NlaIII CATG 4 cut(s) 73, 139, 349, 446
NlaIV GGNNCC 5 cut(s) 85, 145, 156, 272, 507
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 2 cut(s) 697, 747
PagI TCATGA 1 cut(s) 442
PasI CCCWGGG 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PfeI GAWTC 2 cut(s) 216, 380
PfoI TCCNGGA 1 cut(s) 236
PkrI GCNGC 4 cut(s) 175, 285, 358, 427
Ple19I CGATCG 1 cut(s) 334
PleI GAGTC 2 cut(s) 569, 647
PpsI GAGTC 2 cut(s) 569, 647
Ppu21I YACGTR 1 cut(s) 413
PshBI ATTAAT 1 cut(s) 404
Psp6I CCWGG 3 cut(s) 104, 321, 349
PspGI CCWGG 3 cut(s) 104, 321, 349
PspN4I GGNNCC 5 cut(s) 85, 145, 156, 272, 507
PspPI GGNCC 1 cut(s) 154
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 1 cut(s) 505
PvuI CGATCG 1 cut(s) 334
RgaI GCGATCGC 1 cut(s) 334
RsaI GTAC 4 cut(s) 41, 85, 182, 209
RsaNI GTAC 4 cut(s) 40, 84, 181, 208
SaqAI TTAA 2 cut(s) 404, 546
SatI GCNGC 4 cut(s) 174, 284, 357, 426
Sau3AI GATC 5 cut(s) 240, 331, 342, 445, 505
Sau96I GGNCC 1 cut(s) 154
SchI GAGTC 2 cut(s) 569, 647
ScrFI CCNGG 4 cut(s) 106, 238, 323, 351
SduI GDGCHC 2 cut(s) 195, 275
SetI ASST 8 cut(s) 213, 415, 496, 538, 619, 650, 699, 747
SfaAI GCGATCGC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 429
SgfI GCGATCGC 1 cut(s) 334
SinI GGWCC 1 cut(s) 154
Sse9I AATT 4 cut(s) 54, 265, 485, 677
SsiI CCGC 3 cut(s) 93, 174, 286
SspMI CTAG 1 cut(s) 377
StyD4I CCNGG 4 cut(s) 104, 236, 321, 349
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 2 cut(s) 213, 415
TaqI TCGA 6 cut(s) 132, 165, 243, 490, 633, 675
TasI AATT 4 cut(s) 54, 265, 485, 677
TatI WGTACW 1 cut(s) 180
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 2 cut(s) 216, 380
Tru1I TTAA 2 cut(s) 404, 546
Tru9I TTAA 2 cut(s) 404, 546
TseFI GTSAC 2 cut(s) 697, 747
TseI GCWGC 3 cut(s) 283, 356, 425
Tsp45I GTSAC 2 cut(s) 697, 747
TspDTI ATGAA 2 cut(s) 431, 651
TspGWI ACGGA 1 cut(s) 156
VpaK11BI GGWCC 1 cut(s) 154
VspI ATTAAT 1 cut(s) 404
XapI RAATTY 2 cut(s) 485, 677
XbaI TCTAGA 1 cut(s) 376
XcmI CCANNNNNNNNNTGG 2 cut(s) 67, 76
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.