Prupe.1G052300_v2.0.a1

HAD-hyrolase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016053
Physical Location & Seq
Reverse (-)
3644308 .. 3647748
3441 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G052300.1

Sequence Viewer

Length: 759 bp
ATGTCCCTGCTCCTACCCAGAACACTCCTCCTCCTGTACCCAAAACTGAAAATCCCCAAAATACCCATGGCCAGCAATCTCTCCACCCTCAGCTCCGCAGCCCCGCCCAGGGCTCGCCTCCGAGGCGTGGTGTTCGACATGGACGGAACCCTAACGGTCCCCGTCATCGATTTCGCGGCAATGTACAGAGCTGTGCTCGGCGAGGACGAGTACGTTAGAATCAAGGCCGAGAGCCCTTCTGGGATTGATATATTGCACCACATTGAGACTTGGAGCCCCGAGAAGCAGCGAAAGGCGTATGATATTATTGCCGATTACGAGAGCCAGGGGCTCGATCGCCTCCAGATCATGCCTGGTGCAGCAGAACTTTGTGGTTTTCTAGATTCTAAGAATATAAGGAGGGGGTTAATCACACGCAATGTCAAGGCTGCAGTAGATATATTTCATGAACGGTTCAGGTTGACATTTTCTCCAGCTCTGAGCAGGGAATTTCGACCTTACAAACCGGATCCAGCTCCACTACTGCATATATGTTCAGCTTGGGAAGTTAAACCTAGTGAAATAATGATGGTTGGAGATTCCCTTAAAGATGATGTGGCTTGTGGGAAGCGAGCAGGAGCTGTTGCATGCTTGCTTGATGAAAGTGGGAGGTATGACTCTCCAGAATATGCCAACGTCGATTTCAAACCAGATTTCAAGGTGTCTTCCCTATTGGAAATTCACTCGCTATTGGAAGCACATTTTGACCTGTCACCTTGA

Protein Analysis

253

Amino Acids

28.14

Weight (kDa)

6.09

Isoelectric Point (pI)

53.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33255
fragaria_vesca FvH4_4g05140
malus_domestica MD16G1230400.v1.1
prunus_persica Prupe.1G052300_v2.0.a1
pyrus_communis pycom16g19220
rosa_chinensis RchiOBHm_Chr4g0396071
rosa_laevigata RLG00000009575
rosa_multiflora Rmu_sc0006325.1_g000009 Rmu_sc0015594.1_g000003
rosa_roxburghii Rroxscaffold_5G00340870
rosa_rugosa Rorug03G0352800
rosa_samantha Rh4AG065000 Rh4AG065700 Rh4CG070400
rosa_wichuraiana Rw4G005290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 176
AciI CCGC 3 cut(s) 96, 104, 176
AclWI GGATC 2 cut(s) 503, 516
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 2 cut(s) 488, 717
AfaI GTAC 3 cut(s) 38, 185, 212
AfiI CCNNNNNNNGG 3 cut(s) 108, 109, 127
AgsI TTSAA 2 cut(s) 685, 697
AjnI CCWGG 3 cut(s) 107, 324, 352
AjuI GAANNNNNNNTTGG 2 cut(s) 665, 697
AluBI AGCT 6 cut(s) 93, 191, 476, 515, 539, 620
AluI AGCT 6 cut(s) 93, 191, 476, 515, 539, 620
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 2 cut(s) 503, 516
AlwNI CAGNNNCTG 1 cut(s) 620
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 2 cut(s) 69, 225
ApeKI GCWGC 4 cut(s) 98, 286, 359, 428
ApoI RAATTY 2 cut(s) 488, 717
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 744
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 1 cut(s) 157
BalI TGGCCA 1 cut(s) 71
BamHI GGATCC 1 cut(s) 508
BanII GRGCYC 4 cut(s) 115, 236, 278, 333
BbsI GAAGAC 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 198
BbvCI CCTCAGC 1 cut(s) 89
BbvI GCAGC 4 cut(s) 110, 298, 371, 415
BccI CCATC 1 cut(s) 562
BciT130I CCWGG 3 cut(s) 109, 326, 354
BcoDI GTCTC 1 cut(s) 260
BfaI CTAG 2 cut(s) 380, 555
BfmI CTRYAG 1 cut(s) 429
BglI GCCNNNNNGGC 1 cut(s) 123
BisI GCNGC 5 cut(s) 99, 177, 287, 360, 429
BlsI GCNGC 5 cut(s) 100, 178, 288, 361, 430
Bme1390I CCNGG 3 cut(s) 109, 326, 354
Bme18I GGWCC 1 cut(s) 157
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 4 cut(s) 148, 159, 275, 510
BmrFI CCNGG 3 cut(s) 109, 326, 354
BpiI GAAGAC 1 cut(s) 696
BplI GAGNNNNNCTC 2 cut(s) 180, 212
BpmI CTGGAG 3 cut(s) 326, 456, 645
Bpu10I CCTNAGC 1 cut(s) 89
Bsa29I ATCGAT 1 cut(s) 168
BsaJI CCNNGG 5 cut(s) 66, 107, 108, 121, 325
BsaWI WCCGGW 1 cut(s) 505
BsaXI ACNNNNNCTCC 4 cut(s) 15, 45, 454, 484
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 109, 127
Bse3DI GCAATG 2 cut(s) 186, 424
BseBI CCWGG 3 cut(s) 109, 326, 354
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 5 cut(s) 66, 107, 108, 121, 325
BseLI CCNNNNNNNGG 3 cut(s) 108, 109, 127
BseMI GCAATG 2 cut(s) 186, 424
BseMII CTCAG 2 cut(s) 103, 470
BseRI GAGGAG 2 cut(s) 17, 20
BseXI GCAGC 4 cut(s) 110, 298, 371, 415
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 337
BshFI GGCC 2 cut(s) 71, 227
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 1 cut(s) 506
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 3 cut(s) 108, 109, 127
BsmAI GTCTC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 2 cut(s) 71, 227
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 5 cut(s) 115, 198, 236, 278, 333
Bsp1407I TGTACA 1 cut(s) 183
Bsp143I GATC 3 cut(s) 334, 345, 508
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 3 cut(s) 96, 104, 176
BspANI GGCC 2 cut(s) 71, 227
BspCNI CTCAG 2 cut(s) 102, 471
BspDI ATCGAT 1 cut(s) 168
BspFNI CGCG 1 cut(s) 176
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 4 cut(s) 148, 159, 275, 510
BspMAI CTGCAG 1 cut(s) 433
BspPI GGATC 2 cut(s) 503, 516
BsrDI GCAATG 2 cut(s) 186, 424
BsrGI TGTACA 1 cut(s) 183
BssECI CCNNGG 5 cut(s) 66, 107, 108, 121, 325
BssMI GATC 3 cut(s) 334, 345, 508
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 3 cut(s) 109, 326, 354
Bst4CI ACNGT 2 cut(s) 157, 453
BstAUI TGTACA 1 cut(s) 183
BstC8I GCNNGC 5 cut(s) 73, 115, 612, 628, 632
BstDEI CTNAG 3 cut(s) 89, 387, 479
BstDSI CCRYGG 1 cut(s) 66
BstFNI CGCG 1 cut(s) 176
BstKTI GATC 3 cut(s) 337, 348, 511
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 3 cut(s) 334, 345, 508
BstMCI CGRYCG 1 cut(s) 337
BstMWI GCNNNNNNNGC 1 cut(s) 123
BstNI CCWGG 3 cut(s) 109, 326, 354
BstNSI RCATGY 1 cut(s) 630
BstSCI CCNGG 3 cut(s) 107, 324, 352
BstSFI CTRYAG 1 cut(s) 429
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 4 cut(s) 110, 298, 371, 415
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 1 cut(s) 508
BstYI RGATCY 1 cut(s) 508
Bsu15I ATCGAT 1 cut(s) 168
BsuRI GGCC 2 cut(s) 71, 227
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 66
Cac8I GCNNGC 5 cut(s) 73, 115, 612, 628, 632
CaiI CAGNNNCTG 1 cut(s) 620
CciI TCATGA 1 cut(s) 445
Cfr13I GGNCC 1 cut(s) 157
ClaI ATCGAT 1 cut(s) 168
Csp6I GTAC 3 cut(s) 37, 184, 211
CviAII CATG 5 cut(s) 67, 139, 349, 446, 627
CviQI GTAC 3 cut(s) 37, 184, 211
DdeI CTNAG 3 cut(s) 89, 387, 479
DpnI GATC 3 cut(s) 336, 347, 510
DpnII GATC 3 cut(s) 334, 345, 508
EaeI YGGCCR 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 4 cut(s) 115, 236, 278, 333
Eco47I GGWCC 1 cut(s) 157
Eco88I CYCGRG 1 cut(s) 278
EcoRII CCWGG 3 cut(s) 107, 324, 352
EcoT14I CCWWGG 1 cut(s) 66
EcoT38I GRGCYC 4 cut(s) 115, 236, 278, 333
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 5 cut(s) 70, 142, 352, 449, 630
FaqI GGGAC 1 cut(s) 143
FatI CATG 5 cut(s) 66, 138, 348, 445, 626
FauI CCCGC 1 cut(s) 111
Fnu4HI GCNGC 5 cut(s) 99, 177, 287, 360, 429
FriOI GRGCYC 4 cut(s) 115, 236, 278, 333
Fsp4HI GCNGC 5 cut(s) 99, 177, 287, 360, 429
FspBI CTAG 2 cut(s) 380, 555
GluI GCNGC 5 cut(s) 99, 177, 287, 360, 429
GsuI CTGGAG 3 cut(s) 326, 456, 645
HaeIII GGCC 2 cut(s) 71, 227
HapII CCGG 1 cut(s) 506
Hin1II CATG 5 cut(s) 70, 142, 352, 449, 630
HincII GTYRAC 1 cut(s) 462
HindII GTYRAC 1 cut(s) 462
HinfI GANTC 4 cut(s) 219, 383, 578, 656
HpaII CCGG 1 cut(s) 506
HphI GGTGA 1 cut(s) 744
Hpy166II GTNNAC 1 cut(s) 462
Hpy188I TCNGA 2 cut(s) 122, 480
Hpy188III TCNNGA 4 cut(s) 343, 380, 446, 662
Hpy8I GTNNAC 1 cut(s) 462
Hpy99I CGWCG 1 cut(s) 680
HpyAV CCTTC 1 cut(s) 246
HpyCH4III ACNGT 2 cut(s) 157, 453
HpyCH4IV ACGT 2 cut(s) 213, 675
HpyCH4V TGCA 5 cut(s) 256, 359, 431, 526, 626
HpyF10VI GCNNNNNNNGC 1 cut(s) 123
HpyF3I CTNAG 3 cut(s) 89, 387, 479
HpySE526I ACGT 2 cut(s) 213, 675
Hsp92II CATG 5 cut(s) 70, 142, 352, 449, 630
Kzo9I GATC 3 cut(s) 334, 345, 508
LmnI GCTCC 5 cut(s) 15, 98, 273, 520, 617
Lsp1109I GCAGC 4 cut(s) 110, 298, 371, 415
MaeI CTAG 2 cut(s) 380, 555
MaeII ACGT 2 cut(s) 213, 675
MaeIII GTNAC 1 cut(s) 750
MalI GATC 3 cut(s) 336, 347, 510
MboI GATC 3 cut(s) 334, 345, 508
MboII GAAGA 1 cut(s) 696
MflI RGATCY 1 cut(s) 508
MhlI GDGCHC 5 cut(s) 115, 198, 236, 278, 333
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 2 cut(s) 488, 717
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 1 cut(s) 650
MmeI TCCRAC 1 cut(s) 553
MnlI CCTC 9 cut(s) 38, 41, 98, 116, 128, 196, 350, 393, 642
Mox20I TGGCCA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 71
MseI TTAA 3 cut(s) 407, 549, 585
Msp20I TGGCCA 1 cut(s) 71
MspI CCGG 1 cut(s) 506
MspR9I CCNGG 3 cut(s) 109, 326, 354
MvaI CCWGG 3 cut(s) 109, 326, 354
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 123
NcoI CCATGG 1 cut(s) 66
NdeII GATC 3 cut(s) 334, 345, 508
NlaIII CATG 5 cut(s) 70, 142, 352, 449, 630
NlaIV GGNNCC 4 cut(s) 148, 159, 275, 510
NmeAIII GCCGAG 2 cut(s) 177, 253
NmuCI GTSAC 1 cut(s) 750
NspI RCATGY 1 cut(s) 630
PaeI GCATGC 1 cut(s) 630
PagI TCATGA 1 cut(s) 445
PasI CCCWGGG 1 cut(s) 108
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 3 cut(s) 219, 383, 578
PkrI GCNGC 5 cut(s) 100, 178, 288, 361, 430
Ple19I CGATCG 1 cut(s) 337
PleI GAGTC 1 cut(s) 650
PpsI GAGTC 1 cut(s) 650
Psp6I CCWGG 3 cut(s) 107, 324, 352
PspGI CCWGG 3 cut(s) 107, 324, 352
PspN4I GGNNCC 4 cut(s) 148, 159, 275, 510
PspPI GGNCC 1 cut(s) 157
PstI CTGCAG 1 cut(s) 433
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 1 cut(s) 508
PvuI CGATCG 1 cut(s) 337
RsaI GTAC 3 cut(s) 38, 185, 212
RsaNI GTAC 3 cut(s) 37, 184, 211
SaqAI TTAA 3 cut(s) 407, 549, 585
SatI GCNGC 5 cut(s) 99, 177, 287, 360, 429
Sau3AI GATC 3 cut(s) 334, 345, 508
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 1 cut(s) 650
ScrFI CCNGG 3 cut(s) 109, 326, 354
SduI GDGCHC 5 cut(s) 115, 198, 236, 278, 333
SfcI CTRYAG 1 cut(s) 429
SinI GGWCC 1 cut(s) 157
SphI GCATGC 1 cut(s) 630
Sse9I AATT 2 cut(s) 488, 717
SsiI CCGC 3 cut(s) 96, 104, 176
SspMI CTAG 2 cut(s) 380, 555
StyD4I CCNGG 3 cut(s) 107, 324, 352
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 2 cut(s) 157, 453
TaiI ACGT 2 cut(s) 216, 678
TaqI TCGA 5 cut(s) 135, 168, 333, 493, 678
TasI AATT 2 cut(s) 488, 717
TatI WGTACW 1 cut(s) 183
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 3 cut(s) 219, 383, 578
Tru1I TTAA 3 cut(s) 407, 549, 585
Tru9I TTAA 3 cut(s) 407, 549, 585
TseFI GTSAC 1 cut(s) 750
TseI GCWGC 4 cut(s) 98, 286, 359, 428
Tsp45I GTSAC 1 cut(s) 750
TspDTI ATGAA 3 cut(s) 434, 462, 654
TspGWI ACGGA 1 cut(s) 159
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 2 cut(s) 488, 717
XbaI TCTAGA 1 cut(s) 379
XceI RCATGY 1 cut(s) 630
XcmI CCANNNNNNNNNTGG 2 cut(s) 64, 350
XspI CTAG 2 cut(s) 380, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.