RchiOBHm_Chr4g0396071

HAD-hyrolase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016053
Physical Location & Seq
Reverse (-)
12087470 .. 12090592
3123 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36844

Sequence Viewer

Length: 774 bp
ATGCCCCAATTCCTGCCCAGAACCACCGCCGCTCTCTTCCTCTCCCCCAAATTCAAAATCCCCAAACTACCCATGGCCGCGGCCCCCAACACCACTGTATCTGTCTCCACCTCCGCCGGGCCGAGGGCTCGCCTCCGGGGCGTGGTGTTCGACATGGACGGAACCCTAACGGTCCCCGTCATCGATTTCTCGGCAATGTACAAAGCCGTGCTCGGCGAGAAGGAGTACATCAGAATCAGGGCCGAGAGTCCTTCCGGGATTGATATTCTGCACCACATTGAGAGCTGGAGCGTCGACAAGCAGCAGAAGGCCTACGACACCGTCGCCGATTTCGAGCGCCAGGGACTCGAACGACTGCAGATCATGCCTGGTGCAGCAGAACTCTGTGGTTTTCTAGATTCTAAAAATATAAGGCGGGGATTAATCACACGCAATGTCAAGAGCGCGGTTGATTTGTTTCATGAACGATTCAGGTTGTCATTTTCTCCAGCACTGAGTAGGGAATTTCGACCTTACAAACCGGATCCGGCTCCATTACTGCATATATGTTCAACCTGGGAAGTTAACCCTAGTGAAGTAATGATGGTTGGAGATTCCCTTAAAGATGACGTGGCTTGTGGGAAGCGAGCAGGAGCATTTGCATGCTTGCTTGATGAAGTGGGGAGGTATGACTCTCCGGAATTTGCCAATGTTGATTACAAACCAGATTTCAAGGTGTCTTCCCTAACGGAAGTCCATTCGATATTGGAAAAACATTTTGACTTGGCACCTTGA

Protein Analysis

257

Amino Acids

28.65

Weight (kDa)

6.6

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 46 - 211 1.7e-17 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 48 - 213 1.3e-13 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 173 - 244 4.6e-10 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33255
fragaria_vesca FvH4_4g05140
malus_domestica MD16G1230400.v1.1
prunus_persica Prupe.1G052300_v2.0.a1
pyrus_communis pycom16g19220
rosa_chinensis RchiOBHm_Chr4g0396071
rosa_laevigata RLG00000009575
rosa_multiflora Rmu_sc0006325.1_g000009 Rmu_sc0015594.1_g000003
rosa_roxburghii Rroxscaffold_5G00340870
rosa_rugosa Rorug03G0352800
rosa_samantha Rh4AG065000 Rh4AG065700 Rh4CG070400
rosa_wichuraiana Rw4G005290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 766
AccBSI CCGCTC 1 cut(s) 32
AccI GTMKAC 1 cut(s) 294
AccII CGCG 2 cut(s) 80, 446
AccIII TCCGGA 1 cut(s) 676
AciI CCGC 7 cut(s) 27, 30, 78, 80, 114, 415, 446
AclWI GGATC 2 cut(s) 518, 531
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 3 cut(s) 50, 503, 680
AfaI GTAC 2 cut(s) 200, 227
AfiI CCNNNNNNNGG 3 cut(s) 117, 123, 142
AgsI TTSAA 3 cut(s) 55, 552, 712
AjiI CACGTC 1 cut(s) 610
AjnI CCWGG 3 cut(s) 339, 367, 554
AluBI AGCT 1 cut(s) 285
AluI AGCT 1 cut(s) 285
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 109
AlwI GGATC 2 cut(s) 518, 531
Aor13HI TCCGGA 1 cut(s) 676
AoxI GGCC 5 cut(s) 75, 81, 119, 240, 309
ApeKI GCWGC 2 cut(s) 301, 374
ApoI RAATTY 3 cut(s) 50, 503, 680
AseI ATTAAT 1 cut(s) 422
AspLEI GCGC 2 cut(s) 339, 446
AspS9I GGNCC 4 cut(s) 82, 119, 172, 240
AsuC2I CCSGG 3 cut(s) 118, 137, 256
AvaII GGWCC 1 cut(s) 172
BaeI ACNNNNGTAYC 2 cut(s) 81, 114
BamHI GGATCC 1 cut(s) 523
BanI GGYRCC 1 cut(s) 766
BanII GRGCYC 1 cut(s) 130
BbsI GAAGAC 1 cut(s) 711
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 313, 386
BccI CCATC 1 cut(s) 577
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 3 cut(s) 341, 369, 556
BcnI CCSGG 3 cut(s) 118, 137, 256
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 2 cut(s) 395, 570
BfmI CTRYAG 1 cut(s) 356
BfoI RGCGCY 1 cut(s) 340
BglI GCCNNNNNGGC 1 cut(s) 138
BisI GCNGC 5 cut(s) 30, 78, 81, 302, 375
BlsI GCNGC 5 cut(s) 31, 79, 82, 303, 376
Bme1390I CCNGG 6 cut(s) 118, 137, 256, 341, 369, 556
Bme18I GGWCC 1 cut(s) 172
BmgBI CACGTC 1 cut(s) 610
BmgT120I GGNCC 4 cut(s) 82, 119, 172, 240
BmiI GGNNCC 6 cut(s) 84, 163, 174, 525, 531, 768
BmrFI CCNGG 6 cut(s) 118, 137, 256, 341, 369, 556
BpiI GAAGAC 1 cut(s) 711
BpmI CTGGAG 2 cut(s) 307, 471
BpuMI CCSGG 3 cut(s) 118, 137, 256
Bsa29I ATCGAT 1 cut(s) 183
BsaJI CCNNGG 6 cut(s) 72, 78, 122, 136, 340, 555
BsaWI WCCGGW 2 cut(s) 520, 676
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 123, 142
Bse3DI GCAATG 2 cut(s) 201, 439
BseAI TCCGGA 1 cut(s) 676
BseBI CCWGG 3 cut(s) 341, 369, 556
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 6 cut(s) 72, 78, 122, 136, 340, 555
BseLI CCNNNNNNNGG 3 cut(s) 117, 123, 142
BseMI GCAATG 2 cut(s) 201, 439
BseMII CTCAG 1 cut(s) 485
BseXI GCAGC 2 cut(s) 313, 386
BsgI GTGCAG 2 cut(s) 254, 393
Bsh1236I CGCG 2 cut(s) 80, 446
BshFI GGCC 5 cut(s) 77, 83, 121, 242, 311
BshNI GGYRCC 1 cut(s) 766
BshVI ATCGAT 1 cut(s) 183
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 6 cut(s) 117, 136, 255, 521, 527, 677
BslFI GGGAC 2 cut(s) 158, 357
BslI CCNNNNNNNGG 3 cut(s) 117, 123, 142
BsmAI GTCTC 1 cut(s) 109
BsmFI GGGAC 2 cut(s) 158, 357
BsnI GGCC 5 cut(s) 77, 83, 121, 242, 311
Bsp1286I GDGCHC 2 cut(s) 130, 213
Bsp13I TCCGGA 1 cut(s) 676
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 2 cut(s) 360, 523
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 7 cut(s) 27, 30, 78, 80, 114, 415, 446
BspANI GGCC 5 cut(s) 77, 83, 121, 242, 311
BspCNI CTCAG 1 cut(s) 486
BspDI ATCGAT 1 cut(s) 183
BspEI TCCGGA 1 cut(s) 676
BspFNI CGCG 2 cut(s) 80, 446
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 6 cut(s) 84, 163, 174, 525, 531, 768
BspMAI CTGCAG 1 cut(s) 360
BspPI GGATC 2 cut(s) 518, 531
BspT107I GGYRCC 1 cut(s) 766
BsrBI CCGCTC 1 cut(s) 32
BsrDI GCAATG 2 cut(s) 201, 439
BsrGI TGTACA 1 cut(s) 198
BssECI CCNNGG 6 cut(s) 72, 78, 122, 136, 340, 555
BssMI GATC 2 cut(s) 360, 523
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 3 cut(s) 341, 369, 556
Bst4CI ACNGT 3 cut(s) 97, 172, 322
Bst6I CTCTTC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 364
BstAUI TGTACA 1 cut(s) 198
BstC8I GCNNGC 4 cut(s) 130, 627, 643, 647
BstDEI CTNAG 1 cut(s) 494
BstDSI CCRYGG 2 cut(s) 72, 78
BstFNI CGCG 2 cut(s) 80, 446
BstH2I RGCGCY 1 cut(s) 340
BstHHI GCGC 2 cut(s) 339, 446
BstKTI GATC 2 cut(s) 363, 526
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 2 cut(s) 360, 523
BstMWI GCNNNNNNNGC 2 cut(s) 138, 364
BstNI CCWGG 3 cut(s) 341, 369, 556
BstNSI RCATGY 1 cut(s) 645
BstSCI CCNGG 6 cut(s) 116, 135, 254, 339, 367, 554
BstSFI CTRYAG 1 cut(s) 356
BstUI CGCG 2 cut(s) 80, 446
BstV1I GCAGC 2 cut(s) 313, 386
BstV2I GAAGAC 1 cut(s) 711
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
Bsu15I ATCGAT 1 cut(s) 183
BsuRI GGCC 5 cut(s) 77, 83, 121, 242, 311
BsuTUI ATCGAT 1 cut(s) 183
BtgI CCRYGG 2 cut(s) 72, 78
BtrI CACGTC 1 cut(s) 610
BtsIMutI CAGTG 2 cut(s) 93, 491
Cac8I GCNNGC 4 cut(s) 130, 627, 643, 647
CciI TCATGA 1 cut(s) 460
CfoI GCGC 2 cut(s) 339, 446
Cfr13I GGNCC 4 cut(s) 82, 119, 172, 240
Cfr42I CCGCGG 1 cut(s) 81
ClaI ATCGAT 1 cut(s) 183
CseI GACGC 1 cut(s) 280
Csp6I GTAC 2 cut(s) 199, 226
CviAII CATG 5 cut(s) 73, 154, 364, 461, 642
CviQI GTAC 2 cut(s) 199, 226
DdeI CTNAG 1 cut(s) 494
DpnI GATC 2 cut(s) 362, 525
DpnII GATC 2 cut(s) 360, 523
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 72
Eco147I AGGCCT 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 172
EcoRII CCWGG 3 cut(s) 339, 367, 554
EcoT14I CCWWGG 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 5 cut(s) 76, 157, 367, 464, 645
FaqI GGGAC 2 cut(s) 158, 357
FatI CATG 5 cut(s) 72, 153, 363, 460, 641
FauI CCCGC 1 cut(s) 408
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 5 cut(s) 30, 78, 81, 302, 375
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 5 cut(s) 30, 78, 81, 302, 375
FspBI CTAG 2 cut(s) 395, 570
GlaI GCGC 2 cut(s) 338, 445
GluI GCNGC 5 cut(s) 30, 78, 81, 302, 375
GsuI CTGGAG 2 cut(s) 307, 471
HaeII RGCGCY 1 cut(s) 340
HaeIII GGCC 5 cut(s) 77, 83, 121, 242, 311
HapII CCGG 6 cut(s) 117, 136, 255, 521, 527, 677
HgaI GACGC 1 cut(s) 280
HhaI GCGC 2 cut(s) 339, 446
Hin1II CATG 5 cut(s) 76, 157, 367, 464, 645
Hin6I GCGC 2 cut(s) 337, 444
HinP1I GCGC 2 cut(s) 337, 444
HincII GTYRAC 2 cut(s) 295, 565
HindII GTYRAC 2 cut(s) 295, 565
HinfI GANTC 7 cut(s) 234, 247, 345, 398, 468, 593, 671
HpaI GTTAAC 1 cut(s) 565
HpaII CCGG 6 cut(s) 117, 136, 255, 521, 527, 677
Hpy166II GTNNAC 2 cut(s) 295, 565
Hpy188I TCNGA 1 cut(s) 233
Hpy188III TCNNGA 4 cut(s) 395, 439, 461, 677
Hpy8I GTNNAC 2 cut(s) 295, 565
Hpy99I CGWCG 2 cut(s) 296, 326
HpyAV CCTTC 3 cut(s) 214, 261, 301
HpyCH4III ACNGT 3 cut(s) 97, 172, 322
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 5 cut(s) 271, 358, 374, 541, 641
HpyF10VI GCNNNNNNNGC 2 cut(s) 138, 364
HpyF3I CTNAG 1 cut(s) 494
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 5 cut(s) 76, 157, 367, 464, 645
HspAI GCGC 2 cut(s) 337, 444
Kpn2I TCCGGA 1 cut(s) 676
KspAI GTTAAC 1 cut(s) 565
KspI CCGCGG 1 cut(s) 81
Kzo9I GATC 2 cut(s) 360, 523
LmnI GCTCC 3 cut(s) 288, 535, 632
Lsp1109I GCAGC 2 cut(s) 313, 386
MaeI CTAG 2 cut(s) 395, 570
MaeII ACGT 1 cut(s) 609
MalI GATC 2 cut(s) 362, 525
MbiI CCGCTC 1 cut(s) 32
MboI GATC 2 cut(s) 360, 523
MboII GAAGA 2 cut(s) 28, 711
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 2 cut(s) 130, 213
MluCI AATT 4 cut(s) 8, 50, 503, 680
MlyI GAGTC 3 cut(s) 256, 339, 665
MmeI TCCRAC 1 cut(s) 568
MnlI CCTC 5 cut(s) 50, 117, 121, 143, 657
MroI TCCGGA 1 cut(s) 676
MseI TTAA 3 cut(s) 422, 564, 600
MslI CAYNNNNRTG 1 cut(s) 640
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 6 cut(s) 117, 136, 255, 521, 527, 677
MspR9I CCNGG 6 cut(s) 118, 137, 256, 341, 369, 556
MvaI CCWGG 3 cut(s) 341, 369, 556
MvnI CGCG 2 cut(s) 80, 446
MwoI GCNNNNNNNGC 2 cut(s) 138, 364
NciI CCSGG 3 cut(s) 118, 137, 256
NcoI CCATGG 1 cut(s) 72
NdeII GATC 2 cut(s) 360, 523
NlaIII CATG 5 cut(s) 76, 157, 367, 464, 645
NlaIV GGNNCC 6 cut(s) 84, 163, 174, 525, 531, 768
NmeAIII GCCGAG 4 cut(s) 147, 170, 192, 268
NspI RCATGY 1 cut(s) 645
PaeI GCATGC 1 cut(s) 645
PagI TCATGA 1 cut(s) 460
PceI AGGCCT 1 cut(s) 311
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PfeI GAWTC 4 cut(s) 234, 398, 468, 593
PflFI GACNNNGTC 1 cut(s) 320
PfoI TCCNGGA 1 cut(s) 254
PkrI GCNGC 5 cut(s) 31, 79, 82, 303, 376
PleI GAGTC 3 cut(s) 255, 339, 665
PpsI GAGTC 3 cut(s) 255, 339, 665
PshBI ATTAAT 1 cut(s) 422
Psp6I CCWGG 3 cut(s) 339, 367, 554
PspGI CCWGG 3 cut(s) 339, 367, 554
PspN4I GGNNCC 6 cut(s) 84, 163, 174, 525, 531, 768
PspPI GGNCC 4 cut(s) 82, 119, 172, 240
PstI CTGCAG 1 cut(s) 360
PsuI RGATCY 1 cut(s) 523
PsyI GACNNNGTC 1 cut(s) 320
RsaI GTAC 2 cut(s) 200, 227
RsaNI GTAC 2 cut(s) 199, 226
RseI CAYNNNNRTG 1 cut(s) 640
SacII CCGCGG 1 cut(s) 81
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 3 cut(s) 422, 564, 600
SatI GCNGC 5 cut(s) 30, 78, 81, 302, 375
Sau3AI GATC 2 cut(s) 360, 523
Sau96I GGNCC 4 cut(s) 82, 119, 172, 240
SchI GAGTC 3 cut(s) 256, 339, 665
ScrFI CCNGG 6 cut(s) 118, 137, 256, 341, 369, 556
SduI GDGCHC 2 cut(s) 130, 213
SetI ASST 9 cut(s) 113, 287, 476, 514, 557, 612, 668, 717, 772
SfcI CTRYAG 1 cut(s) 356
Sfr303I CCGCGG 1 cut(s) 81
SgrBI CCGCGG 1 cut(s) 81
SinI GGWCC 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 640
SphI GCATGC 1 cut(s) 645
Sse9I AATT 4 cut(s) 8, 50, 503, 680
SseBI AGGCCT 1 cut(s) 311
SsiI CCGC 7 cut(s) 27, 30, 78, 80, 114, 415, 446
SspMI CTAG 2 cut(s) 395, 570
StuI AGGCCT 1 cut(s) 311
StyD4I CCNGG 6 cut(s) 116, 135, 254, 339, 367, 554
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 3 cut(s) 97, 172, 322
TaiI ACGT 1 cut(s) 612
TaqI TCGA 7 cut(s) 150, 183, 294, 333, 348, 508, 740
TasI AATT 4 cut(s) 8, 50, 503, 680
TatI WGTACW 2 cut(s) 198, 225
TauI GCSGC 3 cut(s) 32, 80, 83
TfiI GAWTC 4 cut(s) 234, 398, 468, 593
Tru1I TTAA 3 cut(s) 422, 564, 600
Tru9I TTAA 3 cut(s) 422, 564, 600
TscAI CASTG 2 cut(s) 100, 498
TseI GCWGC 2 cut(s) 301, 374
TspDTI ATGAA 3 cut(s) 449, 477, 669
TspGWI ACGGA 2 cut(s) 174, 743
TspRI CASTG 2 cut(s) 100, 498
Tth111I GACNNNGTC 1 cut(s) 320
VpaK11BI GGWCC 1 cut(s) 172
VspI ATTAAT 1 cut(s) 422
XapI RAATTY 3 cut(s) 50, 503, 680
XbaI TCTAGA 1 cut(s) 394
XceI RCATGY 1 cut(s) 645
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 294
XspI CTAG 2 cut(s) 395, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.