Rh4AG065700

HAD-hyrolase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016053
Physical Location & Seq
Forward (+)
13878563 .. 13881011
2449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG065700.1

Sequence Viewer

Length: 768 bp
ATGCCCCAATTCCTGCCCAGAACCACCGCCGCTCTCTTCCTCTCCCCCAACTTCAAAATTCCCAAACTACCCATGGCCGCCGCCCCCAACACCACTGTCTCCACCTCCGCCGGGCCGAGGGCTCGCCTCCGGGGCGTGGTGTTCGACATGGACGGAACCCTAACGGTCCCCGTCATCGATTTCTCGGCAATGTACAAAGCCGTGCTCGGCGAGAAGGAGTACATCAGAATCAGGGCCGAGAGTCCTTCCGGGATTGATATTCTGCACCACATTGAGAGCTGGAGCGTCGAGAAGCAGCAGAAGGCCTACGACACCGTCGCCGATTTCGAGCGCCAGGGACTCGAACGACTGCAGATCATGCCCGGTGCAGCAGAACTTTGTGGTTTTCTAGATTCTAAAAATATAAGGCGGGGATTAATCACACGCAATGTCAAGAGCGCGGTTGATTTGTTTCATGAACGATTCAGGTTGTCATTTTCTCCAGCACTGAGTAGGGAATTTCGACCTTACAAACCGGATCCAGCTCCATTACTGCATATATGTTCGGCCTGGGAAGTTAACCCTAGTGAAGTAATGATGGTTGGAGATTCCCTTAAAGATGACGTGGCTTGTGGGAAGCGAGCAGGAGCATTTGCATGCTTGCTTGATGAAGTGGGGAGGTATGACTCTCCGGAATTTGCCAATGTTGATTACAAACCAGATTTCAAGGTGTCTTCCCTAACGGAAGTCCATTCGATATTGGAAAAACATTTTGACTTGGCACCTTGA

Protein Analysis

255

Amino Acids

28.43

Weight (kDa)

6.32

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 44 - 209 5.8e-18 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 46 - 211 7.4e-14 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 171 - 242 3.9e-10 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33255
fragaria_vesca FvH4_4g05140
malus_domestica MD16G1230400.v1.1
prunus_persica Prupe.1G052300_v2.0.a1
pyrus_communis pycom16g19220
rosa_chinensis RchiOBHm_Chr4g0396071
rosa_laevigata RLG00000009575
rosa_multiflora Rmu_sc0006325.1_g000009 Rmu_sc0015594.1_g000003
rosa_roxburghii Rroxscaffold_5G00340870
rosa_rugosa Rorug03G0352800
rosa_samantha Rh4AG065000 Rh4AG065700 Rh4CG070400
rosa_wichuraiana Rw4G005290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 760
AccBSI CCGCTC 1 cut(s) 32
AccII CGCG 1 cut(s) 440
AccIII TCCGGA 1 cut(s) 670
AciI CCGC 7 cut(s) 27, 30, 78, 81, 108, 409, 440
AclWI GGATC 2 cut(s) 512, 525
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 3 cut(s) 57, 497, 674
AfaI GTAC 2 cut(s) 194, 221
AfiI CCNNNNNNNGG 3 cut(s) 111, 117, 136
AgsI TTSAA 2 cut(s) 55, 706
AjiI CACGTC 1 cut(s) 604
AjnI CCWGG 2 cut(s) 333, 548
AluBI AGCT 2 cut(s) 279, 524
AluI AGCT 2 cut(s) 279, 524
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 1 cut(s) 103
AlwI GGATC 2 cut(s) 512, 525
Aor13HI TCCGGA 1 cut(s) 670
AoxI GGCC 5 cut(s) 75, 113, 234, 303, 546
ApeKI GCWGC 2 cut(s) 295, 368
ApoI RAATTY 3 cut(s) 57, 497, 674
AseI ATTAAT 1 cut(s) 416
AspLEI GCGC 2 cut(s) 333, 440
AspS9I GGNCC 3 cut(s) 113, 166, 234
AsuC2I CCSGG 4 cut(s) 112, 131, 250, 363
AvaII GGWCC 1 cut(s) 166
BamHI GGATCC 1 cut(s) 517
BanI GGYRCC 1 cut(s) 760
BanII GRGCYC 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 705
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 2 cut(s) 307, 380
BccI CCATC 1 cut(s) 571
BceAI ACGGC 1 cut(s) 185
BciT130I CCWGG 2 cut(s) 335, 550
BcnI CCSGG 4 cut(s) 112, 131, 250, 363
BcoDI GTCTC 1 cut(s) 103
BfaI CTAG 2 cut(s) 389, 564
BfmI CTRYAG 1 cut(s) 350
BfoI RGCGCY 1 cut(s) 334
BglI GCCNNNNNGGC 1 cut(s) 132
BisI GCNGC 5 cut(s) 30, 78, 81, 296, 369
BlsI GCNGC 5 cut(s) 31, 79, 82, 297, 370
Bme1390I CCNGG 6 cut(s) 112, 131, 250, 335, 363, 550
Bme18I GGWCC 1 cut(s) 166
BmgBI CACGTC 1 cut(s) 604
BmgT120I GGNCC 3 cut(s) 113, 166, 234
BmiI GGNNCC 4 cut(s) 157, 168, 519, 762
BmrFI CCNGG 6 cut(s) 112, 131, 250, 335, 363, 550
BpiI GAAGAC 1 cut(s) 705
BpmI CTGGAG 2 cut(s) 301, 465
BpuMI CCSGG 4 cut(s) 112, 131, 250, 363
Bsa29I ATCGAT 1 cut(s) 177
BsaJI CCNNGG 5 cut(s) 72, 116, 130, 334, 549
BsaWI WCCGGW 2 cut(s) 514, 670
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 117, 136
Bse3DI GCAATG 2 cut(s) 195, 433
BseAI TCCGGA 1 cut(s) 670
BseBI CCWGG 2 cut(s) 335, 550
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 5 cut(s) 72, 116, 130, 334, 549
BseLI CCNNNNNNNGG 3 cut(s) 111, 117, 136
BseMI GCAATG 2 cut(s) 195, 433
BseMII CTCAG 1 cut(s) 479
BseXI GCAGC 2 cut(s) 307, 380
BsgI GTGCAG 2 cut(s) 248, 387
Bsh1236I CGCG 1 cut(s) 440
BshFI GGCC 5 cut(s) 77, 115, 236, 305, 548
BshNI GGYRCC 1 cut(s) 760
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 6 cut(s) 111, 130, 249, 363, 515, 671
BslFI GGGAC 2 cut(s) 152, 351
BslI CCNNNNNNNGG 3 cut(s) 111, 117, 136
BsmAI GTCTC 1 cut(s) 103
BsmFI GGGAC 2 cut(s) 152, 351
BsnI GGCC 5 cut(s) 77, 115, 236, 305, 548
Bsp1286I GDGCHC 2 cut(s) 124, 207
Bsp13I TCCGGA 1 cut(s) 670
Bsp1407I TGTACA 1 cut(s) 192
Bsp143I GATC 2 cut(s) 354, 517
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 7 cut(s) 27, 30, 78, 81, 108, 409, 440
BspANI GGCC 5 cut(s) 77, 115, 236, 305, 548
BspCNI CTCAG 1 cut(s) 480
BspDI ATCGAT 1 cut(s) 177
BspEI TCCGGA 1 cut(s) 670
BspFNI CGCG 1 cut(s) 440
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 4 cut(s) 157, 168, 519, 762
BspMAI CTGCAG 1 cut(s) 354
BspPI GGATC 2 cut(s) 512, 525
BspT107I GGYRCC 1 cut(s) 760
BsrBI CCGCTC 1 cut(s) 32
BsrDI GCAATG 2 cut(s) 195, 433
BsrGI TGTACA 1 cut(s) 192
BssECI CCNNGG 5 cut(s) 72, 116, 130, 334, 549
BssMI GATC 2 cut(s) 354, 517
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 2 cut(s) 335, 550
Bst4CI ACNGT 3 cut(s) 97, 166, 316
Bst6I CTCTTC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 358
BstAUI TGTACA 1 cut(s) 192
BstC8I GCNNGC 4 cut(s) 124, 621, 637, 641
BstDEI CTNAG 1 cut(s) 488
BstDSI CCRYGG 1 cut(s) 72
BstFNI CGCG 1 cut(s) 440
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 2 cut(s) 333, 440
BstKTI GATC 2 cut(s) 357, 520
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 2 cut(s) 354, 517
BstMWI GCNNNNNNNGC 2 cut(s) 132, 358
BstNI CCWGG 2 cut(s) 335, 550
BstNSI RCATGY 1 cut(s) 639
BstSCI CCNGG 6 cut(s) 110, 129, 248, 333, 361, 548
BstSFI CTRYAG 1 cut(s) 350
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 2 cut(s) 307, 380
BstV2I GAAGAC 1 cut(s) 705
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 5 cut(s) 77, 115, 236, 305, 548
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 1 cut(s) 72
BtrI CACGTC 1 cut(s) 604
BtsIMutI CAGTG 2 cut(s) 93, 485
Cac8I GCNNGC 4 cut(s) 124, 621, 637, 641
CciI TCATGA 1 cut(s) 454
CfoI GCGC 2 cut(s) 333, 440
Cfr13I GGNCC 3 cut(s) 113, 166, 234
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 193, 220
CviAII CATG 5 cut(s) 73, 148, 358, 455, 636
CviQI GTAC 2 cut(s) 193, 220
DdeI CTNAG 1 cut(s) 488
DpnI GATC 2 cut(s) 356, 519
DpnII GATC 2 cut(s) 354, 517
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 72
Eco147I AGGCCT 1 cut(s) 305
Eco24I GRGCYC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 166
EcoRII CCWGG 2 cut(s) 333, 548
EcoT14I CCWWGG 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 5 cut(s) 76, 151, 361, 458, 639
FaqI GGGAC 2 cut(s) 152, 351
FatI CATG 5 cut(s) 72, 147, 357, 454, 635
FauI CCCGC 1 cut(s) 402
Fnu4HI GCNGC 5 cut(s) 30, 78, 81, 296, 369
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 5 cut(s) 30, 78, 81, 296, 369
FspBI CTAG 2 cut(s) 389, 564
GlaI GCGC 2 cut(s) 332, 439
GluI GCNGC 5 cut(s) 30, 78, 81, 296, 369
GsuI CTGGAG 2 cut(s) 301, 465
HaeII RGCGCY 1 cut(s) 334
HaeIII GGCC 5 cut(s) 77, 115, 236, 305, 548
HapII CCGG 6 cut(s) 111, 130, 249, 363, 515, 671
HgaI GACGC 1 cut(s) 274
HhaI GCGC 2 cut(s) 333, 440
Hin1II CATG 5 cut(s) 76, 151, 361, 458, 639
Hin6I GCGC 2 cut(s) 331, 438
HinP1I GCGC 2 cut(s) 331, 438
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 7 cut(s) 228, 241, 339, 392, 462, 587, 665
HpaI GTTAAC 1 cut(s) 559
HpaII CCGG 6 cut(s) 111, 130, 249, 363, 515, 671
Hpy166II GTNNAC 1 cut(s) 559
Hpy188I TCNGA 1 cut(s) 227
Hpy188III TCNNGA 5 cut(s) 289, 389, 433, 455, 671
Hpy8I GTNNAC 1 cut(s) 559
Hpy99I CGWCG 2 cut(s) 290, 320
HpyAV CCTTC 3 cut(s) 208, 255, 295
HpyCH4III ACNGT 3 cut(s) 97, 166, 316
HpyCH4IV ACGT 1 cut(s) 603
HpyCH4V TGCA 5 cut(s) 265, 352, 368, 535, 635
HpyF10VI GCNNNNNNNGC 2 cut(s) 132, 358
HpyF3I CTNAG 1 cut(s) 488
HpySE526I ACGT 1 cut(s) 603
Hsp92II CATG 5 cut(s) 76, 151, 361, 458, 639
HspAI GCGC 2 cut(s) 331, 438
Kpn2I TCCGGA 1 cut(s) 670
KspAI GTTAAC 1 cut(s) 559
Kzo9I GATC 2 cut(s) 354, 517
LmnI GCTCC 3 cut(s) 282, 529, 626
Lsp1109I GCAGC 2 cut(s) 307, 380
MaeI CTAG 2 cut(s) 389, 564
MaeII ACGT 1 cut(s) 603
MalI GATC 2 cut(s) 356, 519
MbiI CCGCTC 1 cut(s) 32
MboI GATC 2 cut(s) 354, 517
MboII GAAGA 2 cut(s) 28, 705
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 2 cut(s) 124, 207
MluCI AATT 4 cut(s) 8, 57, 497, 674
MlyI GAGTC 3 cut(s) 250, 333, 659
MmeI TCCRAC 1 cut(s) 562
MnlI CCTC 5 cut(s) 50, 111, 115, 137, 651
MroI TCCGGA 1 cut(s) 670
MseI TTAA 3 cut(s) 416, 558, 594
MslI CAYNNNNRTG 1 cut(s) 634
MspI CCGG 6 cut(s) 111, 130, 249, 363, 515, 671
MspR9I CCNGG 6 cut(s) 112, 131, 250, 335, 363, 550
MvaI CCWGG 2 cut(s) 335, 550
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 132, 358
NciI CCSGG 4 cut(s) 112, 131, 250, 363
NcoI CCATGG 1 cut(s) 72
NdeII GATC 2 cut(s) 354, 517
NlaIII CATG 5 cut(s) 76, 151, 361, 458, 639
NlaIV GGNNCC 4 cut(s) 157, 168, 519, 762
NmeAIII GCCGAG 4 cut(s) 141, 164, 186, 262
NspI RCATGY 1 cut(s) 639
PaeI GCATGC 1 cut(s) 639
PagI TCATGA 1 cut(s) 454
PceI AGGCCT 1 cut(s) 305
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PfeI GAWTC 4 cut(s) 228, 392, 462, 587
PflFI GACNNNGTC 1 cut(s) 314
PfoI TCCNGGA 1 cut(s) 248
PkrI GCNGC 5 cut(s) 31, 79, 82, 297, 370
PleI GAGTC 3 cut(s) 249, 333, 659
PpsI GAGTC 3 cut(s) 249, 333, 659
PshBI ATTAAT 1 cut(s) 416
Psp6I CCWGG 2 cut(s) 333, 548
PspGI CCWGG 2 cut(s) 333, 548
PspN4I GGNNCC 4 cut(s) 157, 168, 519, 762
PspPI GGNCC 3 cut(s) 113, 166, 234
PstI CTGCAG 1 cut(s) 354
PsuI RGATCY 1 cut(s) 517
PsyI GACNNNGTC 1 cut(s) 314
RsaI GTAC 2 cut(s) 194, 221
RsaNI GTAC 2 cut(s) 193, 220
RseI CAYNNNNRTG 1 cut(s) 634
SaqAI TTAA 3 cut(s) 416, 558, 594
SatI GCNGC 5 cut(s) 30, 78, 81, 296, 369
Sau3AI GATC 2 cut(s) 354, 517
Sau96I GGNCC 3 cut(s) 113, 166, 234
SchI GAGTC 3 cut(s) 250, 333, 659
ScrFI CCNGG 6 cut(s) 112, 131, 250, 335, 363, 550
SduI GDGCHC 2 cut(s) 124, 207
SetI ASST 9 cut(s) 107, 281, 470, 508, 526, 606, 662, 711, 766
SfcI CTRYAG 1 cut(s) 350
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 634
SphI GCATGC 1 cut(s) 639
Sse9I AATT 4 cut(s) 8, 57, 497, 674
SseBI AGGCCT 1 cut(s) 305
SsiI CCGC 7 cut(s) 27, 30, 78, 81, 108, 409, 440
SspMI CTAG 2 cut(s) 389, 564
StuI AGGCCT 1 cut(s) 305
StyD4I CCNGG 6 cut(s) 110, 129, 248, 333, 361, 548
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 3 cut(s) 97, 166, 316
TaiI ACGT 1 cut(s) 606
TaqI TCGA 7 cut(s) 144, 177, 288, 327, 342, 502, 734
TasI AATT 4 cut(s) 8, 57, 497, 674
TatI WGTACW 2 cut(s) 192, 219
TauI GCSGC 3 cut(s) 32, 80, 83
TfiI GAWTC 4 cut(s) 228, 392, 462, 587
Tru1I TTAA 3 cut(s) 416, 558, 594
Tru9I TTAA 3 cut(s) 416, 558, 594
TscAI CASTG 2 cut(s) 100, 492
TseI GCWGC 2 cut(s) 295, 368
TspDTI ATGAA 3 cut(s) 443, 471, 663
TspGWI ACGGA 2 cut(s) 168, 737
TspRI CASTG 2 cut(s) 100, 492
Tth111I GACNNNGTC 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 166
VspI ATTAAT 1 cut(s) 416
XapI RAATTY 3 cut(s) 57, 497, 674
XbaI TCTAGA 1 cut(s) 388
XceI RCATGY 1 cut(s) 639
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XspI CTAG 2 cut(s) 389, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.