MD16G1238400.v1.1

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015591
Physical Location & Seq
Forward (+)
25166850 .. 25170604
3755 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1238400.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGAGAACATTTCCGGGAAAAAGCGACCCACAATCAGTCTCTCACTTTTTACTACTTTTTCCGAGTCAATTTCCTCTGACAAGTCCCCAAATGCAATCAAATCCCCCCGAAATTTCCAAGAGGGGGTTGTTGGGCTTGGAATAGTGGCTGCCATGACTGATTTGGGCAACCCCAATGAAGTGGCTTTGTCTGACAAGTCGCCTAGGTCGAGCCCGATCCCCATAGTCTCTGCAGCTAAGCCCGCAGCTAATTTCAGAGGTGGGTTTTGTGTGGAGAGGGCAGGGGCTGTGGTGGATGAATTGTCCGAGAGCTATACATGTGTGATTACGCATTTCGGAAACAATTTGACATCGAAAAGGGTTTATTTCGATGATGAGCTGAGTGGGGTTGTGGATGATCCTAATGCTGGTGTGGTGGTGGCCTCTGGGGTGTTCTATGTTTCTCCACTGAGTGTTGGTGAAGTGGGAAGAGAGTTTTGGGCCTCGGATTTTCTCAGTTGGTGTTATCTTTGCAAGAAGCAGCTTCATGGACTGGACATTTTCATGTACAGGGGTGAGAAAGCATTTTGCAGCGCCGAGTGCCGTGACAAGCACATTCGAAGCGATGATCATAAAGAGAAGTGCAGATCGGAAGCATTGAAATCGCTTGAGTACTCGGTGTCTCCCTGCTCCAGTCCGCTCGTCTTCTTATCCGGGGTTGCTGTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

237

Amino Acids

25.52

Weight (kDa)

5.85

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 161 - 204 4.6e-20 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015591)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06100
malus_domestica MD13G1233400.v1.1 MD16G1238400.v1.1
prunus_persica Prupe.1G064600_v2.0.a1
pyrus_communis pycom13g20600 pycom16g19950
rosa_chinensis RchiOBHm_Chr4g0397681
rosa_laevigata RLG00000009419
rosa_multiflora Rmu_sc0002729.1_g000013
rosa_roxburghii Rroxscaffold_5G00342490
rosa_rugosa Rorug03G0341900
rosa_samantha Rh4AG079800 Rh4BG077800 Rh4CG085800 Rh4DG073000
rosa_wichuraiana Rw4G006540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 679
AciI CCGC 2 cut(s) 243, 677
AclWI GGATC 2 cut(s) 211, 392
AcsI RAATTY 1 cut(s) 112
AdeI CACNNNGTG 1 cut(s) 452
AfaI GTAC 2 cut(s) 548, 653
AfiI CCNNNNNNNGG 2 cut(s) 124, 407
AflIII ACRYGT 1 cut(s) 317
AgsI TTSAA 1 cut(s) 640
AjuI GAANNNNNNNTTGG 2 cut(s) 460, 492
AluBI AGCT 5 cut(s) 236, 248, 312, 379, 523
AluI AGCT 5 cut(s) 236, 248, 312, 379, 523
Alw26I GTCTC 3 cut(s) 44, 232, 666
AlwI GGATC 2 cut(s) 211, 392
AlwNI CAGNNNCTG 1 cut(s) 287
AoxI GGCC 2 cut(s) 420, 480
ApeKI GCWGC 5 cut(s) 149, 233, 245, 520, 570
ApoI RAATTY 1 cut(s) 112
AspA2I CCTAGG 1 cut(s) 203
AspLEI GCGC 1 cut(s) 575
AspS9I GGNCC 1 cut(s) 480
AsuC2I CCSGG 2 cut(s) 16, 694
AsuHPI GGTGA 2 cut(s) 470, 566
AsuII TTCGAA 1 cut(s) 598
AvrII CCTAGG 1 cut(s) 203
BanII GRGCYC 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 676
BbvI GCAGC 5 cut(s) 136, 245, 257, 532, 582
BceAI ACGGC 1 cut(s) 567
BcgI CGANNNNNNTGC 2 cut(s) 624, 658
BclI TGATCA 1 cut(s) 607
BcnI CCSGG 2 cut(s) 16, 694
BcoDI GTCTC 3 cut(s) 44, 232, 666
BfaI CTAG 1 cut(s) 204
BfmI CTRYAG 1 cut(s) 231
BfoI RGCGCY 1 cut(s) 576
BisI GCNGC 5 cut(s) 150, 234, 246, 521, 571
BlnI CCTAGG 1 cut(s) 203
BlpI GCTNAGC 1 cut(s) 237
BlsI GCNGC 5 cut(s) 151, 235, 247, 522, 572
BmcAI AGTACT 1 cut(s) 653
Bme1390I CCNGG 2 cut(s) 16, 694
BmgT120I GGNCC 1 cut(s) 480
BmrFI CCNGG 2 cut(s) 16, 694
BpiI GAAGAC 1 cut(s) 676
BpmI CTGGAG 1 cut(s) 655
Bpu1102I GCTNAGC 1 cut(s) 237
Bpu14I TTCGAA 1 cut(s) 598
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 2 cut(s) 16, 694
BsaJI CCNNGG 3 cut(s) 203, 483, 693
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 407
Bse1I ACTGG 2 cut(s) 537, 672
BseDI CCNNGG 3 cut(s) 203, 483, 693
BseGI GGATG 2 cut(s) 301, 400
BseLI CCNNNNNNNGG 2 cut(s) 124, 407
BseMII CTCAG 3 cut(s) 371, 440, 508
BseNI ACTGG 2 cut(s) 537, 672
BseXI GCAGC 5 cut(s) 136, 245, 257, 532, 582
BsgI GTGCAG 1 cut(s) 643
BshFI GGCC 2 cut(s) 422, 482
BsiSI CCGG 2 cut(s) 15, 693
BslFI GGGAC 1 cut(s) 70
BslI CCNNNNNNNGG 2 cut(s) 124, 407
BsmAI GTCTC 3 cut(s) 44, 232, 666
BsmFI GGGAC 1 cut(s) 70
BsnI GGCC 2 cut(s) 422, 482
Bsp119I TTCGAA 1 cut(s) 598
Bsp1286I GDGCHC 1 cut(s) 215
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 4 cut(s) 216, 397, 607, 626
Bsp1720I GCTNAGC 1 cut(s) 237
BspACI CCGC 2 cut(s) 243, 677
BspANI GGCC 2 cut(s) 422, 482
BspCNI CTCAG 3 cut(s) 372, 441, 507
BspMAI CTGCAG 1 cut(s) 235
BspPI GGATC 2 cut(s) 211, 392
BspT104I TTCGAA 1 cut(s) 598
BsrBI CCGCTC 1 cut(s) 679
BsrGI TGTACA 1 cut(s) 546
BsrI ACTGG 2 cut(s) 537, 672
BssECI CCNNGG 3 cut(s) 203, 483, 693
BssMI GATC 4 cut(s) 216, 397, 607, 626
BssT1I CCWWGG 1 cut(s) 203
Bst6I CTCTTC 1 cut(s) 463
BstAUI TGTACA 1 cut(s) 546
BstBI TTCGAA 1 cut(s) 598
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 4 cut(s) 237, 380, 449, 494
BstF5I GGATG 2 cut(s) 301, 400
BstH2I RGCGCY 1 cut(s) 576
BstHHI GCGC 1 cut(s) 575
BstKTI GATC 4 cut(s) 219, 400, 610, 629
BstMAI GTCTC 3 cut(s) 44, 232, 666
BstMBI GATC 4 cut(s) 216, 397, 607, 626
BstMWI GCNNNNNNNGC 2 cut(s) 242, 579
BstNSI RCATGY 1 cut(s) 321
BstSCI CCNGG 2 cut(s) 14, 692
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 5 cut(s) 136, 245, 257, 532, 582
BstV2I GAAGAC 1 cut(s) 676
BstXI CCANNNNNNTGG 1 cut(s) 181
BsuRI GGCC 2 cut(s) 422, 482
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 2 cut(s) 301, 400
BtsIMutI CAGTG 1 cut(s) 446
Cac8I GCNNGC 1 cut(s) 243
CaiI CAGNNNCTG 1 cut(s) 287
CfoI GCGC 1 cut(s) 575
Cfr13I GGNCC 1 cut(s) 480
Csp6I GTAC 2 cut(s) 547, 652
CviAII CATG 5 cut(s) 154, 318, 527, 544, 708
CviQI GTAC 2 cut(s) 547, 652
DdeI CTNAG 4 cut(s) 237, 380, 449, 494
DpnI GATC 4 cut(s) 218, 399, 609, 628
DpnII GATC 4 cut(s) 216, 397, 607, 626
DraIII CACNNNGTG 1 cut(s) 452
Eam1104I CTCTTC 1 cut(s) 463
EarI CTCTTC 1 cut(s) 463
Eco130I CCWWGG 1 cut(s) 203
Eco24I GRGCYC 1 cut(s) 215
EcoT14I CCWWGG 1 cut(s) 203
EcoT38I GRGCYC 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 5 cut(s) 157, 321, 530, 547, 711
FaiI YATR 9 cut(s) 155, 224, 315, 319, 438, 528, 545, 612, 709
FaqI GGGAC 1 cut(s) 70
FatI CATG 5 cut(s) 153, 317, 526, 543, 707
FauI CCCGC 1 cut(s) 250
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 5 cut(s) 150, 234, 246, 521, 571
FokI GGATG 2 cut(s) 308, 407
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 5 cut(s) 150, 234, 246, 521, 571
FspBI CTAG 1 cut(s) 204
GlaI GCGC 1 cut(s) 574
GluI GCNGC 5 cut(s) 150, 234, 246, 521, 571
GsuI CTGGAG 1 cut(s) 655
HaeII RGCGCY 1 cut(s) 576
HaeIII GGCC 2 cut(s) 422, 482
HapII CCGG 2 cut(s) 15, 693
HhaI GCGC 1 cut(s) 575
Hin1II CATG 5 cut(s) 157, 321, 530, 547, 711
Hin6I GCGC 1 cut(s) 573
HinP1I GCGC 1 cut(s) 573
HinfI GANTC 1 cut(s) 65
HpaII CCGG 2 cut(s) 15, 693
HphI GGTGA 2 cut(s) 470, 566
Hpy188I TCNGA 8 cut(s) 64, 79, 193, 257, 307, 338, 487, 631
HpyCH4V TGCA 5 cut(s) 95, 233, 513, 570, 624
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 579
HpyF3I CTNAG 4 cut(s) 237, 380, 449, 494
Hsp92II CATG 5 cut(s) 157, 321, 530, 547, 711
HspAI GCGC 1 cut(s) 573
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 4 cut(s) 216, 397, 607, 626
LmnI GCTCC 1 cut(s) 674
LpnPI CCDG 9 cut(s) 28, 267, 393, 411, 518, 535, 679, 685, 706
Lsp1109I GCAGC 5 cut(s) 136, 245, 257, 532, 582
MaeI CTAG 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 584
MalI GATC 4 cut(s) 218, 399, 609, 628
MbiI CCGCTC 1 cut(s) 679
MboI GATC 4 cut(s) 216, 397, 607, 626
MboII GAAGA 2 cut(s) 480, 676
MhlI GDGCHC 1 cut(s) 215
MluCI AATT 5 cut(s) 69, 112, 250, 299, 343
MlyI GAGTC 1 cut(s) 74
MnlI CCTC 6 cut(s) 85, 115, 251, 270, 433, 493
MslI CAYNNNNRTG 1 cut(s) 542
MspI CCGG 2 cut(s) 15, 693
MspR9I CCNGG 2 cut(s) 16, 694
MwoI GCNNNNNNNGC 2 cut(s) 242, 579
NciI CCSGG 2 cut(s) 16, 694
NdeII GATC 4 cut(s) 216, 397, 607, 626
NlaIII CATG 5 cut(s) 157, 321, 530, 547, 711
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 1 cut(s) 584
NspI RCATGY 1 cut(s) 321
NspV TTCGAA 1 cut(s) 598
PciI ACATGT 1 cut(s) 317
PcsI WCGNNNNNNNCGW 1 cut(s) 206
PfoI TCCNGGA 1 cut(s) 14
PkrI GCNGC 5 cut(s) 151, 235, 247, 522, 572
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PscI ACATGT 1 cut(s) 317
PspPI GGNCC 1 cut(s) 480
PstI CTGCAG 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 287
RsaI GTAC 2 cut(s) 548, 653
RsaNI GTAC 2 cut(s) 547, 652
RseI CAYNNNNRTG 1 cut(s) 542
SatI GCNGC 5 cut(s) 150, 234, 246, 521, 571
Sau3AI GATC 4 cut(s) 216, 397, 607, 626
Sau96I GGNCC 1 cut(s) 480
ScaI AGTACT 1 cut(s) 653
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 2 cut(s) 16, 694
SduI GDGCHC 1 cut(s) 215
SetI ASST 7 cut(s) 209, 238, 250, 262, 314, 381, 525
SfcI CTRYAG 1 cut(s) 231
SfuI TTCGAA 1 cut(s) 598
SmiMI CAYNNNNRTG 1 cut(s) 542
SmlI CTYRAG 1 cut(s) 647
SmoI CTYRAG 1 cut(s) 647
Sse9I AATT 5 cut(s) 69, 112, 250, 299, 343
SsiI CCGC 2 cut(s) 243, 677
SspMI CTAG 1 cut(s) 204
StyD4I CCNGG 2 cut(s) 14, 692
StyI CCWWGG 1 cut(s) 203
TaqI TCGA 4 cut(s) 209, 353, 369, 598
TasI AATT 5 cut(s) 69, 112, 250, 299, 343
TatI WGTACW 2 cut(s) 546, 651
TscAI CASTG 1 cut(s) 453
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 5 cut(s) 149, 233, 245, 520, 570
Tsp45I GTSAC 1 cut(s) 584
TspDTI ATGAA 4 cut(s) 192, 312, 515, 532
TspRI CASTG 1 cut(s) 453
XapI RAATTY 1 cut(s) 112
XceI RCATGY 1 cut(s) 321
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XmaJI CCTAGG 1 cut(s) 203
XspI CTAG 1 cut(s) 204
ZrmI AGTACT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.