Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0009421

Physical Location & Seq

Genomic Coordinates Forward (+)

28922651 .. 28923971

Gene Structure 1321 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1254000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 543 bp

ATGGTGGGTGCATTCTCAGCCCAAACCACTGCTCTGCACAACCCATCTCTCCCTTCATCATCTGACCTGCATTCAACTTCTCATCTTTCACTCTTCAAGAACTCCAATTCAATTGCCTGTGGGTTTTCTTCAAAGCAAGCCGCTTTTCTCAGAGGCCAATTCCGCAGTAGGCACTTTCTTGCGCTCGATCACAATCGTGCTCCAAGACAACCAGCAGGGCATGTCGTTTCACCACGCTGTGTCCTTCCGCTAACCGAAGAAAATGTGGAGAAAGTCCTGGATGAGGTACGGCCTGGTTTGATGGCTGATGGAGGGAATGTGGCCCTCCATGAGATAGATGGCCTTGTTGTGGTATTAAAGCTACAAGGAGCTTGTGGATCTTGTCCAAGCTCAACTATGACGCTGAAGATGGGAATCGAAACCCGCCTGCGAGATAAAATACCCGAGATTATGGAAGTAGAACAGATTTTGGACAGAGAAACAGGCCTTGAGCTAAATGAAGAAAATGTTGAGAAGGTTACAGAAATCATCATTACCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0015979 GO:0016043 GO:0019684 GO:0022607 GO:0032947 GO:0034622 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048564 GO:0065003 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NifU

Protein Analysis

181

Amino Acids

19.61

Weight (kDa)

5.79

Isoelectric Point (pI)

52.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NifU	PF01106	89 - 154	1.4e-26	NifU-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0009421)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25910
fragaria_vesca	FvH4_4g07770 FvH4_4g07770 FvH4_4g07770
malus_domestica	MD16G1254000.v1.1
prunus_persica	Prupe.1G081800_v2.0.a1 Prupe.1G081800_v2.0.a1 Prupe.1G081800_v2.0.a1 Prupe.1G081800_v2.0.a1
pyrus_communis	pycom16g21590
rosa_chinensis	RchiOBHm_Chr4g0400851
rosa_laevigata	RLG00000009172
rosa_multiflora	Rmu_sc0004401.1_g000015 Rmu_sc0018346.1_g000004
rosa_roxburghii	Rroxscaffold_5G00345740
rosa_rugosa	Rorug04G0026300 Rorug04G0026400
rosa_samantha	Rh4AG104400 Rh4BG099600 Rh4CG112800 Rh4DG096500
rosa_wichuraiana	Rw4G008450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	75
AciI	CCGC	4 cut(s)	141, 163, 248, 424
AclWI	GGATC	1 cut(s)	385
AcuI	CTGAAG	1 cut(s)	425
AdeI	CACNNNGTG	1 cut(s)	239
AfaI	GTAC	1 cut(s)	288
AfiI	CCNNNNNNNGG	2 cut(s)	283, 349
AgsI	TTSAA	4 cut(s)	75, 97, 111, 132
AjnI	CCWGG	2 cut(s)	276, 292
AleI	CACNNNNGTG	1 cut(s)	195
AluBI	AGCT	4 cut(s)	361, 371, 390, 493
AluI	AGCT	4 cut(s)	361, 371, 390, 493
Alw21I	GWGCWC	1 cut(s)	202
AlwI	GGATC	1 cut(s)	385
Ama87I	CYCGRG	1 cut(s)	443
AoxI	GGCC	5 cut(s)	154, 290, 321, 340, 484
AspLEI	GCGC	1 cut(s)	184
AspS9I	GGNCC	1 cut(s)	322
AsuHPI	GGTGA	1 cut(s)	222
AvaI	CYCGRG	1 cut(s)	443
Bbv12I	GWGCWC	1 cut(s)	202
BccI	CCATC	5 cut(s)	52, 295, 302, 332, 403
BceAI	ACGGC	1 cut(s)	305
BciT130I	CCWGG	2 cut(s)	278, 294
BfuAI	ACCTGC	1 cut(s)	75
BisI	GCNGC	1 cut(s)	141
BlsI	GCNGC	1 cut(s)	142
Bme1390I	CCNGG	2 cut(s)	278, 294
BmeT110I	CYCGRG	1 cut(s)	443
BmgT120I	GGNCC	1 cut(s)	322
BmrFI	CCNGG	2 cut(s)	278, 294
BpuEI	CTTGAG	1 cut(s)	509
BsaBI	GATNNNNATC	2 cut(s)	192, 413
BsaXI	ACNNNNNCTCC	2 cut(s)	303, 333
Bsc4I	CCNNNNNNNGG	2 cut(s)	283, 349
Bse8I	GATNNNNATC	2 cut(s)	192, 413
BseBI	CCWGG	2 cut(s)	278, 294
BseGI	GGATG	1 cut(s)	286
BseJI	GATNNNNATC	2 cut(s)	192, 413
BseLI	CCNNNNNNNGG	2 cut(s)	283, 349
BseMII	CTCAG	2 cut(s)	30, 163
BsgI	GTGCAG	1 cut(s)	20
BshFI	GGCC	5 cut(s)	156, 292, 323, 342, 486
BsiHKAI	GWGCWC	1 cut(s)	202
BsiHKCI	CYCGRG	1 cut(s)	443
BslI	CCNNNNNNNGG	2 cut(s)	283, 349
BsmI	GAATGC	2 cut(s)	11, 70
BsnI	GGCC	5 cut(s)	156, 292, 323, 342, 486
BsoBI	CYCGRG	1 cut(s)	443
Bsp1286I	GDGCHC	1 cut(s)	202
Bsp143I	GATC	2 cut(s)	187, 377
BspACI	CCGC	4 cut(s)	141, 163, 248, 424
BspANI	GGCC	5 cut(s)	156, 292, 323, 342, 486
BspCNI	CTCAG	2 cut(s)	29, 162
BspMI	ACCTGC	1 cut(s)	75
BspPI	GGATC	1 cut(s)	385
BssMI	GATC	2 cut(s)	187, 377
Bst2UI	CCWGG	2 cut(s)	278, 294
Bst6I	CTCTTC	1 cut(s)	98
BstC8I	GCNNGC	2 cut(s)	138, 428
BstDEI	CTNAG	2 cut(s)	16, 149
BstENI	CCTNNNNNAGG	1 cut(s)	281
BstF5I	GGATG	1 cut(s)	286
BstHHI	GCGC	1 cut(s)	184
BstKTI	GATC	2 cut(s)	190, 380
BstMBI	GATC	2 cut(s)	187, 377
BstMWI	GCNNNNNNNGC	2 cut(s)	17, 162
BstNI	CCWGG	2 cut(s)	278, 294
BstNSI	RCATGY	1 cut(s)	224
BstSCI	CCNGG	2 cut(s)	276, 292
BstX2I	RGATCY	1 cut(s)	377
BstYI	RGATCY	1 cut(s)	377
BsuRI	GGCC	5 cut(s)	156, 292, 323, 342, 486
BtsCI	GGATG	1 cut(s)	286
BtsI	GCAGTG	1 cut(s)	27
BtsIMutI	CAGTG	1 cut(s)	27
BveI	ACCTGC	1 cut(s)	75
Cac8I	GCNNGC	2 cut(s)	138, 428
CfoI	GCGC	1 cut(s)	184
Cfr13I	GGNCC	1 cut(s)	322
CseI	GACGC	1 cut(s)	409
Csp6I	GTAC	1 cut(s)	287
CviAII	CATG	2 cut(s)	221, 329
CviQI	GTAC	1 cut(s)	287
DdeI	CTNAG	2 cut(s)	16, 149
DpnI	GATC	2 cut(s)	189, 379
DpnII	GATC	2 cut(s)	187, 377
DraIII	CACNNNGTG	1 cut(s)	239
Eam1104I	CTCTTC	1 cut(s)	98
EarI	CTCTTC	1 cut(s)	98
Eco147I	AGGCCT	1 cut(s)	486
Eco57I	CTGAAG	1 cut(s)	425
Eco88I	CYCGRG	1 cut(s)	443
EcoNI	CCTNNNNNAGG	1 cut(s)	281
EcoRII	CCWGG	2 cut(s)	276, 292
FaeI	CATG	2 cut(s)	224, 332
FaiI	YATR	4 cut(s)	222, 330, 398, 452
FatI	CATG	2 cut(s)	220, 328
FauI	CCCGC	1 cut(s)	431
Fnu4HI	GCNGC	1 cut(s)	141
FokI	GGATG	1 cut(s)	293
Fsp4HI	GCNGC	1 cut(s)	141
GlaI	GCGC	1 cut(s)	183
GluI	GCNGC	1 cut(s)	141
HaeIII	GGCC	5 cut(s)	156, 292, 323, 342, 486
HgaI	GACGC	1 cut(s)	409
HhaI	GCGC	1 cut(s)	184
Hin1II	CATG	2 cut(s)	224, 332
Hin6I	GCGC	1 cut(s)	182
HinP1I	GCGC	1 cut(s)	182
HinfI	GANTC	1 cut(s)	414
HphI	GGTGA	1 cut(s)	222
Hpy188I	TCNGA	2 cut(s)	64, 152
Hpy188III	TCNNGA	1 cut(s)	97
HpyAV	CCTTC	3 cut(s)	63, 254, 508
HpyCH4V	TGCA	3 cut(s)	11, 37, 70
HpyF10VI	GCNNNNNNNGC	2 cut(s)	17, 162
HpyF3I	CTNAG	2 cut(s)	16, 149
Hsp92II	CATG	2 cut(s)	224, 332
HspAI	GCGC	1 cut(s)	182
Kzo9I	GATC	2 cut(s)	187, 377
LmnI	GCTCC	2 cut(s)	205, 368
MaeIII	GTNAC	1 cut(s)	517
MalI	GATC	2 cut(s)	189, 379
MboI	GATC	2 cut(s)	187, 377
MboII	GAAGA	5 cut(s)	85, 120, 269, 418, 512
MfeI	CAATTG	1 cut(s)	111
MflI	RGATCY	1 cut(s)	377
MhlI	GDGCHC	1 cut(s)	202
MluCI	AATT	3 cut(s)	106, 111, 158
MnlI	CCTC	4 cut(s)	146, 277, 305, 335
MseI	TTAA	1 cut(s)	356
MslI	CAYNNNNRTG	1 cut(s)	195
MspR9I	CCNGG	2 cut(s)	278, 294
MunI	CAATTG	1 cut(s)	111
Mva1269I	GAATGC	2 cut(s)	11, 70
MvaI	CCWGG	2 cut(s)	278, 294
MwoI	GCNNNNNNNGC	2 cut(s)	17, 162
NdeII	GATC	2 cut(s)	187, 377
NlaIII	CATG	2 cut(s)	224, 332
NspI	RCATGY	1 cut(s)	224
OliI	CACNNNNGTG	1 cut(s)	195
PceI	AGGCCT	1 cut(s)	486
PctI	GAATGC	2 cut(s)	11, 70
PfeI	GAWTC	1 cut(s)	414
PfoI	TCCNGGA	1 cut(s)	276
PkrI	GCNGC	1 cut(s)	142
Psp6I	CCWGG	2 cut(s)	276, 292
PspGI	CCWGG	2 cut(s)	276, 292
PspPI	GGNCC	1 cut(s)	322
PsuI	RGATCY	1 cut(s)	377
RsaI	GTAC	1 cut(s)	288
RsaNI	GTAC	1 cut(s)	287
RseI	CAYNNNNRTG	1 cut(s)	195
SaqAI	TTAA	1 cut(s)	356
SatI	GCNGC	1 cut(s)	141
Sau3AI	GATC	2 cut(s)	187, 377
Sau96I	GGNCC	1 cut(s)	322
ScrFI	CCNGG	2 cut(s)	278, 294
SduI	GDGCHC	1 cut(s)	202
SetI	ASST	7 cut(s)	69, 288, 363, 373, 392, 495, 519
SmiMI	CAYNNNNRTG	1 cut(s)	195
SmlI	CTYRAG	1 cut(s)	488
SmoI	CTYRAG	1 cut(s)	488
Sse9I	AATT	3 cut(s)	106, 111, 158
SseBI	AGGCCT	1 cut(s)	486
SsiI	CCGC	4 cut(s)	141, 163, 248, 424
StuI	AGGCCT	1 cut(s)	486
StyD4I	CCNGG	2 cut(s)	276, 292
TaqI	TCGA	2 cut(s)	186, 417
TasI	AATT	3 cut(s)	106, 111, 158
TauI	GCSGC	1 cut(s)	143
TfiI	GAWTC	1 cut(s)	414
Tru1I	TTAA	1 cut(s)	356
Tru9I	TTAA	1 cut(s)	356
TscAI	CASTG	1 cut(s)	34
TspDTI	ATGAA	2 cut(s)	45, 513
TspRI	CASTG	1 cut(s)	34
XagI	CCTNNNNNAGG	1 cut(s)	281
XceI	RCATGY	1 cut(s)	224
XcmI	CCANNNNNNNNNTGG	1 cut(s)	335

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.