MD16G1264800.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0018023
Physical Location & Seq
Forward (+)
33574336 .. 33576441
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1264800.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGGGATCATCCTTGAGTGCTTGCTGTAGTTCAGAGAAGGTAGACGAAGAAGGGGCTACTCGTGAAGTTGGTGGAGGAGGTCACACATCATGGAGAATATTTTCGTACAAGGAGTTGCATACAGCTACTCATGGGTTTAGTGATGACAATTTGCTTGGAGAGGGAGGGTTTGGAAGTGTTTATTGGGGAAAGACCAGTGATGGTCTACAGATTGCTGTGAAGAAGTTGAAAGCAATGAATTCAAAAGCTGAAATGGAATTTGCAGTTGAGGTTGAAGTTCTTGGAAGGGTTAGGCACAAGAATTTGTTGGGACTTAGGGGCTATTATGCTGGGGCAGATCAACGCCTTATTGTCTATGATTACATGCCAAATCTTAGTTTACTTTCTCACTTGCATGGCCAATTTGCAAGTGAAGTTCGGCTGGATTGGAAAAGGAGAATGAAAGTTGCTATTGGCTCAGCAGAAGGCCTCCTGTACTTACACCATGAGGTGACACCCCACATCATTCATAGAGATATAAAGGCTAGCAATGTGCTGTTGGATTCAGATTTCGAACCACTAGTTGCAGACTTTGGGTTTGCCAAGCTAATCCCCGAAGGTGTGAGCCACATGACTACTCGAGTCAAGGGAACTTTGGGTTACCTAGCACCTGAATATGCCATGTGGGGAAAAGTCTCAGAGAGCTGTGATGTTTATAGTTTTGGGATTCTTCTTTTAGAGCTCGTAACTGGAAGAAAGCCTATAGAAAAACTCCCTGGCGGAGTTAAGAGGACGATCACTGAATGGGCGGAGCCACTTATAACCAAAGGCAGACTTAAGGAACTTGCAGACCCAAGACTCAGAGGAAACTTTGATGAAGGCCAACTGAAGCAAGCTATCAATGTTGCTGCACTTTGTGTGAAAAGTGAGCCTGAAAAAAGGCTCAAGATTAAGGAAGTGGTAGATCTTTTAAAAGGGTATGAGGCTCAAATCAAGATGAAACCATTGAGAATTGAAAGTGTGAGATACAAGGAGGAGCTTCTGGCCCTCGATCAAGCTACCGATGATGATGAAGATGGAGGGGCGGACGAAAGTACTAACGGGTATGGAGTTTTCAGTGCTATAGAGGTGCAGAAAATGCAGGATCCTTACAAGGAAAGGAGAACGGCGAAATATGTGTGA

Protein Analysis

387

Amino Acids

42.98

Weight (kDa)

6.3

Isoelectric Point (pI)

30.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 48 - 314 1.9e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 50 - 315 1.8e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018023)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08480
malus_domestica MD16G1264800.v1.1
prunus_persica Prupe.1G094300_v2.0.a1
pyrus_communis pycom16g23410
rosa_chinensis RchiOBHm_Chr4g0403901
rosa_laevigata RLG00000008943
rosa_multiflora Rmu_sc0005134.1_g000012
rosa_roxburghii Rroxscaffold_5G00348480
rosa_rugosa Rorug04G0046100
rosa_samantha Rh4BG114800
rosa_wichuraiana Rw4G009900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 800
AccI GTMKAC 2 cut(s) 42, 205
AciI CCGC 3 cut(s) 759, 788, 1064
AclWI GGATC 3 cut(s) 13, 1118, 1131
AcoI YGGCCR 1 cut(s) 397
AcsI RAATTY 3 cut(s) 238, 257, 301
AcuI CTGAAG 1 cut(s) 887
AdeI CACNNNGTG 1 cut(s) 896
AfaI GTAC 3 cut(s) 107, 476, 1075
AflII CTTAAG 1 cut(s) 815
AgsI TTSAA 4 cut(s) 229, 243, 275, 995
AhlI ACTAGT 1 cut(s) 559
AjnI CCWGG 1 cut(s) 754
AjuI GAANNNNNNNTTGG 2 cut(s) 166, 198
AluBI AGCT 8 cut(s) 125, 248, 586, 684, 721, 875, 1018, 1037
AluI AGCT 8 cut(s) 125, 248, 586, 684, 721, 875, 1018, 1037
Alw21I GWGCWC 1 cut(s) 723
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 3 cut(s) 13, 1118, 1131
Ama87I CYCGRG 1 cut(s) 618
AoxI GGCC 4 cut(s) 397, 466, 859, 1023
ApeKI GCWGC 1 cut(s) 887
ApoI RAATTY 3 cut(s) 238, 257, 301
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
Asp700I GAANNNNTTC 1 cut(s) 100
AspS9I GGNCC 1 cut(s) 1024
AsuHPI GGTGA 1 cut(s) 502
AsuII TTCGAA 1 cut(s) 552
AsuNHI GCTAGC 1 cut(s) 524
AvaI CYCGRG 1 cut(s) 618
BalI TGGCCA 1 cut(s) 399
BamHI GGATCC 1 cut(s) 1123
BanII GRGCYC 1 cut(s) 723
BauI CACGAG 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 723
BbvI GCAGC 1 cut(s) 874
BccI CCATC 2 cut(s) 194, 1049
BciT130I CCWGG 1 cut(s) 756
BcoDI GTCTC 1 cut(s) 679
BcuI ACTAGT 1 cut(s) 559
BfaI CTAG 3 cut(s) 525, 560, 644
BfmI CTRYAG 4 cut(s) 25, 206, 741, 1101
BfrI CTTAAG 1 cut(s) 815
BglII AGATCT 1 cut(s) 943
BisI GCNGC 1 cut(s) 888
BlpI GCTNAGC 1 cut(s) 457
BlsI GCNGC 1 cut(s) 889
BmcAI AGTACT 1 cut(s) 1075
Bme1390I CCNGG 1 cut(s) 756
BmeT110I CYCGRG 1 cut(s) 618
BmgT120I GGNCC 1 cut(s) 1024
BmiI GGNNCC 2 cut(s) 792, 1125
BmrFI CCNGG 1 cut(s) 756
BmtI GCTAGC 1 cut(s) 528
Bpu1102I GCTNAGC 1 cut(s) 457
Bpu14I TTCGAA 1 cut(s) 552
BpuEI CTTGAG 2 cut(s) 34, 908
BsaJI CCNNGG 1 cut(s) 754
Bse1I ACTGG 2 cut(s) 195, 733
Bse3DI GCAATG 2 cut(s) 240, 535
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 1 cut(s) 754
BseGI GGATG 1 cut(s) 8
BseMI GCAATG 2 cut(s) 240, 535
BseMII CTCAG 3 cut(s) 471, 690, 853
BseNI ACTGG 2 cut(s) 195, 733
BseRI GAGGAG 2 cut(s) 90, 1028
BseXI GCAGC 1 cut(s) 874
BseYI CCCAGC 1 cut(s) 329
BsgI GTGCAG 2 cut(s) 873, 1130
BshFI GGCC 4 cut(s) 399, 468, 861, 1025
BsiHKAI GWGCWC 1 cut(s) 723
BsiHKCI CYCGRG 1 cut(s) 618
BslFI GGGAC 1 cut(s) 324
BsmAI GTCTC 1 cut(s) 679
BsmFI GGGAC 1 cut(s) 324
BsnI GGCC 4 cut(s) 399, 468, 861, 1025
BsoBI CYCGRG 1 cut(s) 618
Bsp119I TTCGAA 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 723
Bsp143I GATC 6 cut(s) 5, 337, 774, 943, 1030, 1123
Bsp1720I GCTNAGC 1 cut(s) 457
BspACI CCGC 3 cut(s) 759, 788, 1064
BspANI GGCC 4 cut(s) 399, 468, 861, 1025
BspCNI CTCAG 3 cut(s) 470, 689, 852
BspLI GGNNCC 2 cut(s) 792, 1125
BspOI GCTAGC 1 cut(s) 528
BspPI GGATC 3 cut(s) 13, 1118, 1131
BspT104I TTCGAA 1 cut(s) 552
BspTI CTTAAG 1 cut(s) 815
BsrDI GCAATG 2 cut(s) 240, 535
BsrI ACTGG 2 cut(s) 195, 733
BssECI CCNNGG 1 cut(s) 754
BssMI GATC 6 cut(s) 5, 337, 774, 943, 1030, 1123
BssSI CACGAG 1 cut(s) 60
Bst2BI CACGAG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 756
BstAFI CTTAAG 1 cut(s) 815
BstAPI GCANNNNNTGC 1 cut(s) 1117
BstBI TTCGAA 1 cut(s) 552
BstC8I GCNNGC 3 cut(s) 22, 526, 873
BstDEI CTNAG 5 cut(s) 314, 374, 457, 676, 839
BstEII GGTNACC 1 cut(s) 638
BstF5I GGATG 1 cut(s) 8
BstKTI GATC 6 cut(s) 8, 340, 777, 946, 1033, 1126
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 6 cut(s) 5, 337, 774, 943, 1030, 1123
BstMWI GCNNNNNNNGC 1 cut(s) 1117
BstNI CCWGG 1 cut(s) 756
BstNSI RCATGY 1 cut(s) 367
BstPI GGTNACC 1 cut(s) 638
BstSCI CCNGG 1 cut(s) 754
BstSFI CTRYAG 4 cut(s) 25, 206, 741, 1101
BstV1I GCAGC 1 cut(s) 874
BstX2I RGATCY 2 cut(s) 943, 1123
BstYI RGATCY 2 cut(s) 943, 1123
BsuRI GGCC 4 cut(s) 399, 468, 861, 1025
BtsCI GGATG 1 cut(s) 8
BtsIMutI CAGTG 3 cut(s) 202, 777, 1102
Cac8I GCNNGC 3 cut(s) 22, 526, 873
Cfr13I GGNCC 1 cut(s) 1024
Csp6I GTAC 3 cut(s) 106, 475, 1074
CviAII CATG 7 cut(s) 90, 131, 364, 395, 485, 610, 661
CviQI GTAC 3 cut(s) 106, 475, 1074
DdeI CTNAG 5 cut(s) 314, 374, 457, 676, 839
DpnI GATC 6 cut(s) 7, 339, 776, 945, 1032, 1125
DpnII GATC 6 cut(s) 5, 337, 774, 943, 1030, 1123
DraI TTTAAA 1 cut(s) 951
DraIII CACNNNGTG 1 cut(s) 896
EaeI YGGCCR 1 cut(s) 397
EciI GGCGGA 3 cut(s) 774, 803, 1079
Ecl136II GAGCTC 1 cut(s) 721
Eco147I AGGCCT 1 cut(s) 468
Eco24I GRGCYC 1 cut(s) 723
Eco53kI GAGCTC 1 cut(s) 721
Eco57I CTGAAG 1 cut(s) 887
Eco88I CYCGRG 1 cut(s) 618
Eco91I GGTNACC 1 cut(s) 638
EcoICRI GAGCTC 1 cut(s) 721
EcoO65I GGTNACC 1 cut(s) 638
EcoRI GAATTC 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 754
EcoT38I GRGCYC 1 cut(s) 723
FaeI CATG 7 cut(s) 93, 134, 367, 398, 488, 613, 664
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FaqI GGGAC 1 cut(s) 324
FatI CATG 7 cut(s) 89, 130, 363, 394, 484, 609, 660
FblI GTMKAC 2 cut(s) 42, 205
Fnu4HI GCNGC 1 cut(s) 888
FriOI GRGCYC 1 cut(s) 723
Fsp4HI GCNGC 1 cut(s) 888
FspBI CTAG 3 cut(s) 525, 560, 644
GluI GCNGC 1 cut(s) 888
GsaI CCCAGC 1 cut(s) 333
HaeIII GGCC 4 cut(s) 399, 468, 861, 1025
Hin1II CATG 7 cut(s) 93, 134, 367, 398, 488, 613, 664
HinfI GANTC 4 cut(s) 542, 621, 706, 837
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 3 cut(s) 43, 206, 380
Hpy188I TCNGA 4 cut(s) 34, 547, 679, 842
Hpy188III TCNNGA 3 cut(s) 62, 925, 973
Hpy8I GTNNAC 3 cut(s) 43, 206, 380
HpyAV CCTTC 6 cut(s) 31, 44, 279, 458, 590, 851
HpyCH4V TGCA 9 cut(s) 118, 263, 394, 407, 566, 827, 890, 1111, 1120
HpyF10VI GCNNNNNNNGC 1 cut(s) 1117
HpyF3I CTNAG 5 cut(s) 314, 374, 457, 676, 839
Hsp92II CATG 7 cut(s) 93, 134, 367, 398, 488, 613, 664
Kzo9I GATC 6 cut(s) 5, 337, 774, 943, 1030, 1123
LmnI GCTCC 2 cut(s) 790, 1015
Lsp1109I GCAGC 1 cut(s) 874
MaeI CTAG 3 cut(s) 525, 560, 644
MaeIII GTNAC 4 cut(s) 80, 490, 638, 724
MalI GATC 6 cut(s) 7, 339, 776, 945, 1032, 1125
MboI GATC 6 cut(s) 5, 337, 774, 943, 1030, 1123
MboII GAAGA 5 cut(s) 59, 232, 701, 744, 1064
MflI RGATCY 2 cut(s) 943, 1123
MhlI GDGCHC 1 cut(s) 723
MlsI TGGCCA 1 cut(s) 399
MluCI AATT 6 cut(s) 148, 238, 257, 301, 401, 990
MluNI TGGCCA 1 cut(s) 399
MlyI GAGTC 2 cut(s) 630, 831
MmeI TCCRAC 1 cut(s) 519
Mox20I TGGCCA 1 cut(s) 399
MroXI GAANNNNTTC 1 cut(s) 100
MscI TGGCCA 1 cut(s) 399
MseI TTAA 4 cut(s) 765, 816, 930, 950
MslI CAYNNNNRTG 1 cut(s) 393
Msp20I TGGCCA 1 cut(s) 399
MspCI CTTAAG 1 cut(s) 815
MspR9I CCNGG 1 cut(s) 756
MvaI CCWGG 1 cut(s) 756
MwoI GCNNNNNNNGC 1 cut(s) 1117
NdeII GATC 6 cut(s) 5, 337, 774, 943, 1030, 1123
NheI GCTAGC 1 cut(s) 524
NlaIII CATG 7 cut(s) 93, 134, 367, 398, 488, 613, 664
NlaIV GGNNCC 2 cut(s) 792, 1125
NmuCI GTSAC 2 cut(s) 80, 490
NspI RCATGY 1 cut(s) 367
NspV TTCGAA 1 cut(s) 552
PaeR7I CTCGAG 1 cut(s) 618
PceI AGGCCT 1 cut(s) 468
PdmI GAANNNNTTC 1 cut(s) 100
PfeI GAWTC 2 cut(s) 542, 706
PkrI GCNGC 1 cut(s) 889
PleI GAGTC 2 cut(s) 629, 831
PpsI GAGTC 2 cut(s) 629, 831
PsiI TTATAA 1 cut(s) 800
Psp124BI GAGCTC 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 754
PspEI GGTNACC 1 cut(s) 638
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 2 cut(s) 792, 1125
PspPI GGNCC 1 cut(s) 1024
PspXI VCTCGAGB 1 cut(s) 618
PsuI RGATCY 2 cut(s) 943, 1123
RsaI GTAC 3 cut(s) 107, 476, 1075
RsaNI GTAC 3 cut(s) 106, 475, 1074
RseI CAYNNNNRTG 1 cut(s) 393
SacI GAGCTC 1 cut(s) 723
SaqAI TTAA 4 cut(s) 765, 816, 930, 950
SatI GCNGC 1 cut(s) 888
Sau3AI GATC 6 cut(s) 5, 337, 774, 943, 1030, 1123
Sau96I GGNCC 1 cut(s) 1024
ScaI AGTACT 1 cut(s) 1075
SchI GAGTC 2 cut(s) 630, 831
ScrFI CCNGG 1 cut(s) 756
SduI GDGCHC 1 cut(s) 723
SfcI CTRYAG 4 cut(s) 25, 206, 741, 1101
Sfr274I CTCGAG 1 cut(s) 618
SfuI TTCGAA 1 cut(s) 552
SlaI CTCGAG 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 393
SmlI CTYRAG 4 cut(s) 13, 618, 815, 923
SmoI CTYRAG 4 cut(s) 13, 618, 815, 923
SpeI ACTAGT 1 cut(s) 559
Sse9I AATT 6 cut(s) 148, 238, 257, 301, 401, 990
SseBI AGGCCT 1 cut(s) 468
SsiI CCGC 3 cut(s) 759, 788, 1064
SspI AATATT 1 cut(s) 99
SspMI CTAG 3 cut(s) 525, 560, 644
SstI GAGCTC 1 cut(s) 723
StuI AGGCCT 1 cut(s) 468
StyD4I CCNGG 1 cut(s) 754
TaqI TCGA 3 cut(s) 552, 619, 1029
TasI AATT 6 cut(s) 148, 238, 257, 301, 401, 990
TatI WGTACW 2 cut(s) 474, 1073
TfiI GAWTC 2 cut(s) 542, 706
Tru1I TTAA 4 cut(s) 765, 816, 930, 950
Tru9I TTAA 4 cut(s) 765, 816, 930, 950
TscAI CASTG 3 cut(s) 202, 784, 1102
TseFI GTSAC 2 cut(s) 80, 490
TseI GCWGC 1 cut(s) 887
Tsp45I GTSAC 2 cut(s) 80, 490
TspDTI ATGAA 6 cut(s) 251, 455, 497, 870, 992, 1065
TspRI CASTG 3 cut(s) 202, 784, 1102
Vha464I CTTAAG 1 cut(s) 815
XapI RAATTY 3 cut(s) 238, 257, 301
XceI RCATGY 1 cut(s) 367
XhoI CTCGAG 1 cut(s) 618
XmiI GTMKAC 2 cut(s) 42, 205
XmnI GAANNNNTTC 1 cut(s) 100
XspI CTAG 3 cut(s) 525, 560, 644
ZrmI AGTACT 1 cut(s) 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.