MD16G1269400.v1.1

13-prostaglandin reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016611
Physical Location & Seq
Forward (+)
34791274 .. 34791561
288 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1269400.v1.1.491

Sequence Viewer

Length: 288 bp
ATGGGTCTGCTCTCGTGGTGGAAAGGGAATGAGTCTACACCCCTAACCAAGACCAGCCCGGATCCGAAACCGGCCGAACCTGCTCCCGGCATGAACGGCGCCGTTGAGGTGCCTCGACCCCCCGCCGCCGCCAACATCACCGTCTTCGAGTTCGGATCAGTCGCGGCTTCAGCCGATAAGGTCACTCTCGCCGGGTACTGTCCGGTCTCCGAAGACCTGGAGCCCTGCCGCTGGGAGATCCTCCCCGCTAGTGGCTCCGACGCGCCCCAGTTTCGAGTGGTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

96

Amino Acids

10.03

Weight (kDa)

4.58

Isoelectric Point (pI)

53.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016611)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59840
malus_domestica MD16G1269400.v1.1
prunus_persica Prupe.5G046200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0208331
rosa_laevigata RLG00000003226
rosa_multiflora Rmu_sc0000841.1_g000010 Rmu_sc0007869.1_g000030
rosa_roxburghii Rroxscaffold_3G00250560
rosa_rugosa Rorug07G0103500
rosa_samantha Rh7AG237800 Rh7BG233400 Rh7CG253500 Rh7DG243800
rosa_wichuraiana Rw7G020270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 88
AccB1I GGYRCC 2 cut(s) 98, 109
AccI GTMKAC 1 cut(s) 35
AccII CGCG 2 cut(s) 164, 263
AciI CCGC 6 cut(s) 123, 126, 129, 164, 229, 246
AclWI GGATC 4 cut(s) 56, 69, 163, 232
AcoI YGGCCR 1 cut(s) 72
AcuI CTGAAG 1 cut(s) 153
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 3 cut(s) 86, 231, 251
AjnI CCWGG 1 cut(s) 216
Alw26I GTCTC 1 cut(s) 211
AlwI GGATC 4 cut(s) 56, 69, 163, 232
AoxI GGCC 1 cut(s) 72
AspLEI GCGC 2 cut(s) 101, 265
AsuC2I CCSGG 3 cut(s) 59, 87, 193
AsuHPI GGTGA 1 cut(s) 130
BamHI GGATCC 1 cut(s) 61
BanI GGYRCC 2 cut(s) 98, 109
BanII GRGCYC 1 cut(s) 225
BauI CACGAG 1 cut(s) 13
BbsI GAAGAC 3 cut(s) 136, 219, 274
BceAI ACGGC 2 cut(s) 86, 112
BciT130I CCWGG 1 cut(s) 218
BcnI CCSGG 3 cut(s) 59, 87, 193
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 1 cut(s) 249
BfoI RGCGCY 1 cut(s) 102
BfuAI ACCTGC 1 cut(s) 88
BisI GCNGC 4 cut(s) 126, 129, 165, 229
BlsI GCNGC 4 cut(s) 127, 130, 166, 230
Bme1390I CCNGG 4 cut(s) 59, 87, 193, 218
BmiI GGNNCC 5 cut(s) 63, 100, 111, 222, 256
BmrFI CCNGG 4 cut(s) 59, 87, 193, 218
BmrI ACTGGG 1 cut(s) 262
BmuI ACTGGG 1 cut(s) 262
BpiI GAAGAC 3 cut(s) 136, 219, 274
BpmI CTGGAG 1 cut(s) 239
BpuMI CCSGG 3 cut(s) 59, 87, 193
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 211
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 3 cut(s) 86, 231, 251
Bse118I RCCGGY 1 cut(s) 70
Bse1I ACTGG 1 cut(s) 268
BseBI CCWGG 1 cut(s) 218
BseLI CCNNNNNNNGG 3 cut(s) 86, 231, 251
BseNI ACTGG 1 cut(s) 268
BseX3I CGGCCG 1 cut(s) 72
BseYI CCCAGC 1 cut(s) 231
Bsh1236I CGCG 2 cut(s) 164, 263
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 74
BshNI GGYRCC 2 cut(s) 98, 109
BsiEI CGRYCG 1 cut(s) 75
BsiSI CCGG 5 cut(s) 59, 71, 87, 192, 203
BslI CCNNNNNNNGG 3 cut(s) 86, 231, 251
BsmAI GTCTC 1 cut(s) 211
BsnI GGCC 1 cut(s) 74
Bso31I GGTCTC 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 3 cut(s) 61, 155, 237
BspACI CCGC 6 cut(s) 123, 126, 129, 164, 229, 246
BspANI GGCC 1 cut(s) 74
BspFNI CGCG 2 cut(s) 164, 263
BspLI GGNNCC 5 cut(s) 63, 100, 111, 222, 256
BspMI ACCTGC 1 cut(s) 88
BspPI GGATC 4 cut(s) 56, 69, 163, 232
BspT107I GGYRCC 2 cut(s) 98, 109
BspTNI GGTCTC 1 cut(s) 211
BsrFI RCCGGY 1 cut(s) 70
BsrI ACTGG 1 cut(s) 268
BssAI RCCGGY 1 cut(s) 70
BssMI GATC 3 cut(s) 61, 155, 237
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 1 cut(s) 13
Bst2BI CACGAG 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 2 cut(s) 142, 200
BstACI GRCGYC 1 cut(s) 99
BstFNI CGCG 2 cut(s) 164, 263
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 2 cut(s) 101, 265
BstKTI GATC 3 cut(s) 64, 158, 240
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 3 cut(s) 61, 155, 237
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 3 cut(s) 80, 96, 170
BstNI CCWGG 1 cut(s) 218
BstSCI CCNGG 4 cut(s) 57, 85, 191, 216
BstUI CGCG 2 cut(s) 164, 263
BstV2I GAAGAC 3 cut(s) 136, 219, 274
BstX2I RGATCY 2 cut(s) 61, 237
BstYI RGATCY 2 cut(s) 61, 237
BstZI CGGCCG 1 cut(s) 72
BsuRI GGCC 1 cut(s) 74
BveI ACCTGC 1 cut(s) 88
CfoI GCGC 2 cut(s) 101, 265
Cfr10I RCCGGY 1 cut(s) 70
CseI GACGC 1 cut(s) 269
Csp6I GTAC 1 cut(s) 196
CviAII CATG 1 cut(s) 91
CviJI RGCY 6 cut(s) 57, 74, 167, 173, 223, 255
CviKI_1 RGCY 6 cut(s) 57, 74, 167, 173, 223, 255
CviQI GTAC 1 cut(s) 196
DinI GGCGCC 1 cut(s) 100
DpnI GATC 3 cut(s) 63, 157, 239
DpnII GATC 3 cut(s) 61, 155, 237
EaeI YGGCCR 1 cut(s) 72
EagI CGGCCG 1 cut(s) 72
EclXI CGGCCG 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 211
Eco52I CGGCCG 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 225
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
FaeI CATG 1 cut(s) 94
FaiI YATR 1 cut(s) 92
FatI CATG 1 cut(s) 90
FauI CCCGC 2 cut(s) 130, 253
FblI GTMKAC 1 cut(s) 35
Fnu4HI GCNGC 4 cut(s) 126, 129, 165, 229
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 4 cut(s) 126, 129, 165, 229
FspBI CTAG 1 cut(s) 249
GlaI GCGC 2 cut(s) 100, 264
GluI GCNGC 4 cut(s) 126, 129, 165, 229
GsaI CCCAGC 1 cut(s) 235
GsuI CTGGAG 1 cut(s) 239
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 1 cut(s) 74
HapII CCGG 5 cut(s) 59, 71, 87, 192, 203
HgaI GACGC 1 cut(s) 269
HhaI GCGC 2 cut(s) 101, 265
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 1 cut(s) 94
Hin6I GCGC 2 cut(s) 99, 263
HinP1I GCGC 2 cut(s) 99, 263
HinfI GANTC 1 cut(s) 32
HpaII CCGG 5 cut(s) 59, 71, 87, 192, 203
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 36
Hpy188I TCNGA 4 cut(s) 66, 155, 211, 259
Hpy8I GTNNAC 1 cut(s) 36
Hpy99I CGWCG 1 cut(s) 263
HpyCH4III ACNGT 2 cut(s) 142, 200
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 96, 170
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 1 cut(s) 94
HspAI GCGC 2 cut(s) 99, 263
KasI GGCGCC 1 cut(s) 98
Kzo9I GATC 3 cut(s) 61, 155, 237
LmnI GCTCC 3 cut(s) 88, 220, 260
MaeI CTAG 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 181
MalI GATC 3 cut(s) 63, 157, 239
MboI GATC 3 cut(s) 61, 155, 237
MboII GAAGA 3 cut(s) 136, 224, 274
MflI RGATCY 2 cut(s) 61, 237
MhlI GDGCHC 1 cut(s) 225
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 3 cut(s) 100, 123, 251
MspA1I CMGCKG 1 cut(s) 231
MspI CCGG 5 cut(s) 59, 71, 87, 192, 203
MspR9I CCNGG 4 cut(s) 59, 87, 193, 218
MvaI CCWGG 1 cut(s) 218
MvnI CGCG 2 cut(s) 164, 263
MwoI GCNNNNNNNGC 3 cut(s) 80, 96, 170
NarI GGCGCC 1 cut(s) 99
NciI CCSGG 3 cut(s) 59, 87, 193
NdeII GATC 3 cut(s) 61, 155, 237
NlaIII CATG 1 cut(s) 94
NlaIV GGNNCC 5 cut(s) 63, 100, 111, 222, 256
NmuCI GTSAC 1 cut(s) 181
PkrI GCNGC 4 cut(s) 127, 130, 166, 230
PleI GAGTC 1 cut(s) 40
PluTI GGCGCC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 40
Psp6I CCWGG 1 cut(s) 216
PspFI CCCAGC 1 cut(s) 231
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 5 cut(s) 63, 100, 111, 222, 256
PsuI RGATCY 2 cut(s) 61, 237
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SatI GCNGC 4 cut(s) 126, 129, 165, 229
Sau3AI GATC 3 cut(s) 61, 155, 237
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 4 cut(s) 59, 87, 193, 218
SduI GDGCHC 1 cut(s) 225
SetI ASST 4 cut(s) 82, 111, 183, 219
SfoI GGCGCC 1 cut(s) 100
SsiI CCGC 6 cut(s) 123, 126, 129, 164, 229, 246
SspDI GGCGCC 1 cut(s) 98
SspMI CTAG 1 cut(s) 249
StyD4I CCNGG 4 cut(s) 57, 85, 191, 216
TaaI ACNGT 2 cut(s) 142, 200
TaqI TCGA 3 cut(s) 115, 147, 274
TauI GCSGC 4 cut(s) 128, 131, 167, 231
TseFI GTSAC 1 cut(s) 181
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 35
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.