Rw7G020270

13-prostaglandin reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016611
Physical Location & Seq
Forward (+)
22873015 .. 22874545
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G020270.1

Sequence Viewer

Length: 282 bp
ATGGGTCTTTTCTTCTGGCGGAAAGGCAGCGACTCCAAAGCCGAAACGAAGCCCAACCCGAACCCGGATCCGAAACCGGTTGAGGTTCCGGGCATGAACGGCGCCGTTGAGGTCCCACGGCCCTCCAAAGTCACCGTCTTTGAATTCGGGTCGGTTGCCGCTTCCGCAGATAAGGTCACTCTCGCCGGGTACTGCCCCGTTTCGGAGGAGCTCGAGCCCTGCCGCTGGGAGATCCTCTCCGCGAGTGGCTCCGACGCGCCGCAGTTCCGCGTGGTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

93

Amino Acids

10.04

Weight (kDa)

5.05

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016611)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59840
malus_domestica MD16G1269400.v1.1
prunus_persica Prupe.5G046200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0208331
rosa_laevigata RLG00000003226
rosa_multiflora Rmu_sc0000841.1_g000010 Rmu_sc0007869.1_g000030
rosa_roxburghii Rroxscaffold_3G00250560
rosa_rugosa Rorug07G0103500
rosa_samantha Rh7AG237800 Rh7BG233400 Rh7CG253500 Rh7DG243800
rosa_wichuraiana Rw7G020270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 101
AccII CGCG 3 cut(s) 242, 257, 270
AciI CCGC 7 cut(s) 19, 159, 165, 223, 240, 260, 268
AclWI GGATC 3 cut(s) 62, 75, 226
AcsI RAATTY 1 cut(s) 143
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 1 cut(s) 191
AfiI CCNNNNNNNGG 3 cut(s) 64, 202, 225
AgeI ACCGGT 1 cut(s) 76
AgsI TTSAA 1 cut(s) 143
AluBI AGCT 1 cut(s) 211
AluI AGCT 1 cut(s) 211
Alw21I GWGCWC 1 cut(s) 213
AlwI GGATC 3 cut(s) 62, 75, 226
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 1 cut(s) 119
ApeKI GCWGC 1 cut(s) 27
ApoI RAATTY 1 cut(s) 143
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AsiGI ACCGGT 1 cut(s) 76
AspLEI GCGC 2 cut(s) 104, 259
AspS9I GGNCC 2 cut(s) 112, 120
AsuC2I CCSGG 3 cut(s) 65, 90, 187
AsuHPI GGTGA 1 cut(s) 124
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 1 cut(s) 112
BamHI GGATCC 1 cut(s) 67
BanI GGYRCC 1 cut(s) 101
BanII GRGCYC 2 cut(s) 213, 219
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 39
BceAI ACGGC 3 cut(s) 89, 115, 134
BcnI CCSGG 3 cut(s) 65, 90, 187
BfoI RGCGCY 1 cut(s) 105
BisI GCNGC 4 cut(s) 28, 159, 223, 260
BlsI GCNGC 4 cut(s) 29, 160, 224, 261
Bme1390I CCNGG 3 cut(s) 65, 90, 187
Bme18I GGWCC 1 cut(s) 112
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 112, 120
BmiI GGNNCC 5 cut(s) 69, 87, 103, 114, 250
BmrFI CCNGG 3 cut(s) 65, 90, 187
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpuMI CCSGG 3 cut(s) 65, 90, 187
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 116
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 202, 225
Bse118I RCCGGY 1 cut(s) 76
BseDI CCNNGG 1 cut(s) 116
BseLI CCNNNNNNNGG 3 cut(s) 64, 202, 225
BseRI GAGGAG 1 cut(s) 221
BseXI GCAGC 1 cut(s) 39
BseYI CCCAGC 1 cut(s) 225
Bsh1236I CGCG 3 cut(s) 242, 257, 270
BshFI GGCC 1 cut(s) 121
BshNI GGYRCC 1 cut(s) 101
BshTI ACCGGT 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 4 cut(s) 65, 77, 89, 186
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 3 cut(s) 64, 202, 225
BsmFI GGGAC 1 cut(s) 98
BsnI GGCC 1 cut(s) 121
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 2 cut(s) 213, 219
Bsp143I GATC 2 cut(s) 67, 231
BspACI CCGC 7 cut(s) 19, 159, 165, 223, 240, 260, 268
BspANI GGCC 1 cut(s) 121
BspFNI CGCG 3 cut(s) 242, 257, 270
BspLI GGNNCC 5 cut(s) 69, 87, 103, 114, 250
BspPI GGATC 3 cut(s) 62, 75, 226
BspT107I GGYRCC 1 cut(s) 101
BsrFI RCCGGY 1 cut(s) 76
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 116
BssMI GATC 2 cut(s) 67, 231
BssNI GRCGYC 1 cut(s) 102
Bst4CI ACNGT 1 cut(s) 136
BstACI GRCGYC 1 cut(s) 102
BstDSI CCRYGG 1 cut(s) 116
BstFNI CGCG 3 cut(s) 242, 257, 270
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 2 cut(s) 104, 259
BstKTI GATC 2 cut(s) 70, 234
BstMBI GATC 2 cut(s) 67, 231
BstMWI GCNNNNNNNGC 2 cut(s) 99, 164
BstSCI CCNGG 3 cut(s) 63, 88, 185
BstUI CGCG 3 cut(s) 242, 257, 270
BstV1I GCAGC 1 cut(s) 39
BstX2I RGATCY 2 cut(s) 67, 231
BstYI RGATCY 2 cut(s) 67, 231
BsuRI GGCC 1 cut(s) 121
BtgI CCRYGG 1 cut(s) 116
CfoI GCGC 2 cut(s) 104, 259
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 112, 120
CseI GACGC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 190
CspAI ACCGGT 1 cut(s) 76
CviAII CATG 1 cut(s) 94
CviJI RGCY 6 cut(s) 41, 52, 121, 211, 217, 249
CviKI_1 RGCY 6 cut(s) 41, 52, 121, 211, 217, 249
CviQI GTAC 1 cut(s) 190
DinI GGCGCC 1 cut(s) 103
DpnI GATC 2 cut(s) 69, 233
DpnII GATC 2 cut(s) 67, 231
EciI GGCGGA 1 cut(s) 34
Ecl136II GAGCTC 1 cut(s) 211
Eco24I GRGCYC 2 cut(s) 213, 219
Eco47I GGWCC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 212
EcoICRI GAGCTC 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 112
EcoRI GAATTC 1 cut(s) 143
EcoT38I GRGCYC 2 cut(s) 213, 219
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
FaeI CATG 1 cut(s) 97
FaiI YATR 1 cut(s) 95
FaqI GGGAC 1 cut(s) 98
FatI CATG 1 cut(s) 93
Fnu4HI GCNGC 4 cut(s) 28, 159, 223, 260
FriOI GRGCYC 2 cut(s) 213, 219
Fsp4HI GCNGC 4 cut(s) 28, 159, 223, 260
GlaI GCGC 2 cut(s) 103, 258
GluI GCNGC 4 cut(s) 28, 159, 223, 260
GsaI CCCAGC 1 cut(s) 229
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 1 cut(s) 121
HapII CCGG 4 cut(s) 65, 77, 89, 186
HgaI GACGC 1 cut(s) 263
HhaI GCGC 2 cut(s) 104, 259
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 1 cut(s) 97
Hin6I GCGC 2 cut(s) 102, 257
HinP1I GCGC 2 cut(s) 102, 257
HinfI GANTC 1 cut(s) 32
HpaII CCGG 4 cut(s) 65, 77, 89, 186
HphI GGTGA 1 cut(s) 124
Hpy188I TCNGA 4 cut(s) 72, 205, 253, 281
Hpy99I CGWCG 1 cut(s) 257
HpyCH4III ACNGT 1 cut(s) 136
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 164
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 1 cut(s) 97
HspAI GCGC 2 cut(s) 102, 257
KasI GGCGCC 1 cut(s) 101
Kzo9I GATC 2 cut(s) 67, 231
LmnI GCTCC 2 cut(s) 208, 254
LpnPI CCDG 6 cut(s) 78, 90, 102, 199, 211, 232
Lsp1109I GCAGC 1 cut(s) 39
MaeIII GTNAC 2 cut(s) 130, 175
MalI GATC 2 cut(s) 69, 233
MboI GATC 2 cut(s) 67, 231
MboII GAAGA 1 cut(s) 4
MflI RGATCY 2 cut(s) 67, 231
MhlI GDGCHC 2 cut(s) 213, 219
MluCI AATT 1 cut(s) 143
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 5 cut(s) 76, 103, 133, 199, 245
MspA1I CMGCKG 1 cut(s) 225
MspI CCGG 4 cut(s) 65, 77, 89, 186
MspR9I CCNGG 3 cut(s) 65, 90, 187
MvnI CGCG 3 cut(s) 242, 257, 270
MwoI GCNNNNNNNGC 2 cut(s) 99, 164
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 3 cut(s) 65, 90, 187
NdeII GATC 2 cut(s) 67, 231
NlaIII CATG 1 cut(s) 97
NlaIV GGNNCC 5 cut(s) 69, 87, 103, 114, 250
NmuCI GTSAC 2 cut(s) 130, 175
PaeR7I CTCGAG 1 cut(s) 212
PinAI ACCGGT 1 cut(s) 76
PkrI GCNGC 4 cut(s) 29, 160, 224, 261
PleI GAGTC 1 cut(s) 26
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 112
Psp124BI GAGCTC 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 112
PspFI CCCAGC 1 cut(s) 225
PspN4I GGNNCC 5 cut(s) 69, 87, 103, 114, 250
PspPI GGNCC 2 cut(s) 112, 120
PspPPI RGGWCCY 1 cut(s) 112
PspXI VCTCGAGB 1 cut(s) 212
PsuI RGATCY 2 cut(s) 67, 231
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
SacI GAGCTC 1 cut(s) 213
SatI GCNGC 4 cut(s) 28, 159, 223, 260
Sau3AI GATC 2 cut(s) 67, 231
Sau96I GGNCC 2 cut(s) 112, 120
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 3 cut(s) 65, 90, 187
SduI GDGCHC 2 cut(s) 213, 219
SetI ASST 4 cut(s) 87, 114, 177, 213
SfoI GGCGCC 1 cut(s) 103
Sfr274I CTCGAG 1 cut(s) 212
SinI GGWCC 1 cut(s) 112
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 1 cut(s) 143
SsiI CCGC 7 cut(s) 19, 159, 165, 223, 240, 260, 268
SspDI GGCGCC 1 cut(s) 101
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 3 cut(s) 63, 88, 185
TaaI ACNGT 1 cut(s) 136
TaqI TCGA 1 cut(s) 213
TasI AATT 1 cut(s) 143
TauI GCSGC 3 cut(s) 161, 225, 262
TseFI GTSAC 2 cut(s) 130, 175
TseI GCWGC 1 cut(s) 27
Tsp45I GTSAC 2 cut(s) 130, 175
TspDTI ATGAA 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 143
XhoI CTCGAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.