MD16G1273800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017308
Physical Location & Seq
Reverse (-)
36436868 .. 36437848
981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1273800.v1.1.491

Sequence Viewer

Length: 537 bp
ATGGTTGTTTTCTTCGAAAGAGGAGGGAAGAAGCAGAAAACATTGCGAAAATCTATTCTCAGCTGGAACAGGAGGGCACTTGAAAACGATCTTATGTTGGAGTCACCAGGGTCGATGAACACTCCGAAACATGTACTCATCGTGATGGATGGGATGAAAGAGTTCAGAACAGAGCCGCTTGAATGGGCGCTCGAGAATGTAATCAACGCTGGTTCCACGGTCACTCTTCTTGGTGTCATGCCGTGGCTGAATATCCCCCTCTCTTCCAAGACATGGTTGGATGTTTGGCCAGTGGGGTTAGAAGAGTTATCATTTTTCAAAGAGAGGAACGAGTGGAAGAGTGACATCAAATATCTAAAGCTTCAGAAAGTCGTGGATCTGTGCAACAAATATGGGGTTTTTCCACGGAAGAAAGTTATAATGGGTTATCCCTTACGGCTAATGGTTGTTGAGGAAATTACAACCCTTCATGCGACATGGGTGGTTTTTGACAGGTATAAACTATGTAGTAATATCGAAGACTTTGGATTTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

20.79

Weight (kDa)

9.04

Isoelectric Point (pI)

35.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g22141
malus_domestica MD06G1034800.v1.1 MD16G1273800.v1.1
prunus_persica Prupe.5G035100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0209801
rosa_laevigata RLG00000003089
rosa_roxburghii Rroxscaffold_3G00248960
rosa_rugosa Rorug07G0116300
rosa_samantha Rh7AG250300 Rh7BG244200 Rh7CG266200 Rh7DG255900
rosa_wichuraiana Rw7G021200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 419
AccB7I CCANNNNNTGG 1 cut(s) 273
AciI CCGC 1 cut(s) 176
AclWI GGATC 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 347
AfaI GTAC 1 cut(s) 135
AfiI CCNNNNNNNGG 1 cut(s) 273
AflIII ACRYGT 1 cut(s) 130
AgsI TTSAA 3 cut(s) 83, 182, 319
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 2 cut(s) 63, 361
AluI AGCT 2 cut(s) 63, 361
AlwI GGATC 1 cut(s) 384
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 1 cut(s) 287
Asp700I GAANNNNTTC 1 cut(s) 161
AspLEI GCGC 1 cut(s) 190
AsuHPI GGTGA 1 cut(s) 96
AsuII TTCGAA 1 cut(s) 15
AvaI CYCGRG 1 cut(s) 191
BaeGI GKGCMC 1 cut(s) 79
BalI TGGCCA 1 cut(s) 289
BbsI GAAGAC 1 cut(s) 525
BccI CCATC 2 cut(s) 139, 143
BceAI ACGGC 2 cut(s) 226, 452
BciT130I CCWGG 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 191
BisI GCNGC 1 cut(s) 176
BlsI GCNGC 1 cut(s) 177
Bme1390I CCNGG 1 cut(s) 108
BmeT110I CYCGRG 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 108
BpiI GAAGAC 1 cut(s) 525
Bpu14I TTCGAA 1 cut(s) 15
BsaJI CCNNGG 4 cut(s) 107, 216, 242, 404
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 290
Bse3DI GCAATG 1 cut(s) 41
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 4 cut(s) 107, 216, 242, 404
BseGI GGATG 3 cut(s) 154, 159, 286
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 1 cut(s) 73
BseNI ACTGG 1 cut(s) 290
BseRI GAGGAG 1 cut(s) 36
BseSI GKGCMC 1 cut(s) 79
BshFI GGCC 1 cut(s) 289
BsiHKCI CYCGRG 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 273
BsnI GGCC 1 cut(s) 289
BsoBI CYCGRG 1 cut(s) 191
Bsp119I TTCGAA 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 2 cut(s) 88, 376
BspACI CCGC 1 cut(s) 176
BspANI GGCC 1 cut(s) 289
BspCNI CTCAG 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 214
BspPI GGATC 1 cut(s) 384
BspT104I TTCGAA 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 41
BsrI ACTGG 1 cut(s) 290
BssECI CCNNGG 4 cut(s) 107, 216, 242, 404
BssMI GATC 2 cut(s) 88, 376
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 4 cut(s) 231, 268, 297, 332
BstBI TTCGAA 1 cut(s) 15
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 3 cut(s) 216, 242, 404
BstF5I GGATG 3 cut(s) 154, 159, 286
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 2 cut(s) 91, 379
BstMBI GATC 2 cut(s) 88, 376
BstNI CCWGG 1 cut(s) 108
BstNSI RCATGY 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 106
BstSLI GKGCMC 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 525
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 1 cut(s) 289
BtgI CCRYGG 3 cut(s) 216, 242, 404
BtsCI GGATG 3 cut(s) 154, 159, 286
BtsIMutI CAGTG 1 cut(s) 297
CfoI GCGC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 134
CviAII CATG 5 cut(s) 131, 238, 273, 470, 477
CviJI RGCY 6 cut(s) 63, 175, 247, 289, 361, 439
CviKI_1 RGCY 6 cut(s) 63, 175, 247, 289, 361, 439
CviQI GTAC 1 cut(s) 134
DdeI CTNAG 1 cut(s) 59
DpnI GATC 2 cut(s) 90, 378
DpnII GATC 2 cut(s) 88, 376
EaeI YGGCCR 1 cut(s) 287
Eam1104I CTCTTC 4 cut(s) 231, 268, 297, 332
EarI CTCTTC 4 cut(s) 231, 268, 297, 332
Eco57I CTGAAG 1 cut(s) 347
Eco88I CYCGRG 1 cut(s) 191
EcoRII CCWGG 1 cut(s) 106
FaeI CATG 5 cut(s) 134, 241, 276, 473, 480
FatI CATG 5 cut(s) 130, 237, 272, 469, 476
Fnu4HI GCNGC 1 cut(s) 176
FokI GGATG 3 cut(s) 161, 166, 293
Fsp4HI GCNGC 1 cut(s) 176
GlaI GCGC 1 cut(s) 189
GluI GCNGC 1 cut(s) 176
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 1 cut(s) 289
HhaI GCGC 1 cut(s) 190
Hin1II CATG 5 cut(s) 134, 241, 276, 473, 480
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 1 cut(s) 101
HphI GGTGA 1 cut(s) 96
Hpy188I TCNGA 3 cut(s) 126, 167, 366
Hpy188III TCNNGA 2 cut(s) 142, 193
HpyAV CCTTC 1 cut(s) 476
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4V TGCA 1 cut(s) 384
HpyF3I CTNAG 1 cut(s) 59
Hsp92II CATG 5 cut(s) 134, 241, 276, 473, 480
HspAI GCGC 1 cut(s) 188
Kzo9I GATC 2 cut(s) 88, 376
LpnPI CCDG 7 cut(s) 49, 55, 93, 120, 195, 303, 478
MaeIII GTNAC 3 cut(s) 102, 220, 341
MalI GATC 2 cut(s) 90, 378
MboI GATC 2 cut(s) 88, 376
MboII GAAGA 8 cut(s) 4, 40, 218, 255, 314, 349, 421, 530
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 79
MlsI TGGCCA 1 cut(s) 289
MluCI AATT 1 cut(s) 456
MluNI TGGCCA 1 cut(s) 289
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 2 cut(s) 78, 258
MnlI CCTC 6 cut(s) 14, 17, 66, 269, 318, 445
Mox20I TGGCCA 1 cut(s) 289
MroXI GAANNNNTTC 1 cut(s) 161
MscI TGGCCA 1 cut(s) 289
MslI CAYNNNNRTG 1 cut(s) 143
Msp20I TGGCCA 1 cut(s) 289
MspA1I CMGCKG 1 cut(s) 63
MspR9I CCNGG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 108
NdeII GATC 2 cut(s) 88, 376
NlaIII CATG 5 cut(s) 134, 241, 276, 473, 480
NlaIV GGNNCC 1 cut(s) 214
NmuCI GTSAC 3 cut(s) 102, 220, 341
NspI RCATGY 1 cut(s) 134
NspV TTCGAA 1 cut(s) 15
PaeR7I CTCGAG 1 cut(s) 191
PciI ACATGT 1 cut(s) 130
PdmI GAANNNNTTC 1 cut(s) 161
PflMI CCANNNNNTGG 1 cut(s) 273
PkrI GCNGC 1 cut(s) 177
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PscI ACATGT 1 cut(s) 130
PsiI TTATAA 1 cut(s) 419
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 214
PsuI RGATCY 1 cut(s) 376
PvuII CAGCTG 1 cut(s) 63
RsaI GTAC 1 cut(s) 135
RsaNI GTAC 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 143
SatI GCNGC 1 cut(s) 176
Sau3AI GATC 2 cut(s) 88, 376
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 1 cut(s) 79
SetI ASST 3 cut(s) 65, 363, 497
Sfr274I CTCGAG 1 cut(s) 191
SfuI TTCGAA 1 cut(s) 15
SlaI CTCGAG 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 1 cut(s) 456
SsiI CCGC 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 106
TaaI ACNGT 1 cut(s) 220
TaqI TCGA 4 cut(s) 15, 113, 192, 516
TasI AATT 1 cut(s) 456
TatI WGTACW 1 cut(s) 133
TauI GCSGC 1 cut(s) 178
TscAI CASTG 1 cut(s) 297
TseFI GTSAC 3 cut(s) 102, 220, 341
Tsp45I GTSAC 3 cut(s) 102, 220, 341
TspDTI ATGAA 3 cut(s) 131, 170, 458
TspGWI ACGGA 1 cut(s) 421
TspRI CASTG 1 cut(s) 297
Van91I CCANNNNNTGG 1 cut(s) 273
XceI RCATGY 1 cut(s) 134
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
XhoI CTCGAG 1 cut(s) 191
XmnI GAANNNNTTC 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.