MD17G1011400.v1.1

Regulation of nuclear pre-mRNA domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010397
Physical Location & Seq
Reverse (-)
715955 .. 721479
5525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1011400.v1.1.491

Sequence Viewer

Length: 1557 bp
ATGGGAAGTGCGTTCAATCCACAGATTTTAGTGGAAAAGCTAGCCAAGCTCAATAGTTCGCAAGCAAGCATAGAGACTTTATCACATTGGTGCATTTTCCATATGAATAAAGCAAGACAAGTTGTTGAAACATGGGCTAGGCAATTTCATTGTGCTCCACGCGACCAGAGATTGGCTTTCTTGTATCTTGCAAATGATATTTTACAGAACAGTAGGCGAAAAGGTTCAGAATTTGTTGGTGAATTTTGGAAGGTTCTTCCAGATGCTCTTCGTGATGTAATTAAAAGTGGAGATGAATTTGGAAGAAATGCAGCACAACGATTGGTTAATATATGGGATGATAGAAAAGTTTTTGGTTCACACGGCCAGATTCTCAAGCAAGAGTTTGTTGGACAGAACCCGGAAATTAGTAACAGAAATGGGAAGCATTCGGGTGCCAGATTGCATCCTGCTGGAACTGCGTTGGACAAAATAGTTTCAGATTACCAAGCTAGTCATGGCCAGATGGATGAGGACACTATATTGAGCAAATGCAGGAGTGCTATTAGCTGTCTAGAGAGGGTTGATAAGGAAATTGGTGCCGATTTCAACTCAGGGCAATTTCATGGGTCTGCATTTTTGGAGGAGGTTCAGGGTCAGAATGCTATACTAAGGAATTGTATTGAACAACTAAGAGCTGTTGAATCATCAAGAGCTAATCTTGTGTCTCATCTAAGAGAGGCTCTTCAGGAGCAGGAGTACAAGCTAGATCAAGTCCGAAACCAACTTCAGGATGCAGAGTCCCAGTCAGAGCTGGCCACTAATATCTGCCATCGGTTAATGAATGGCGACAATGCACAACAGATTGCAGTCCAGAGCTCAAAGGAAGCTCATACCTCTGTAGCACCTTCCAGCTTTATGGTAGGAAGAGAACAATCAGCTCCAGTAATGTACACACAGCAGGTTGCTTTTGCTGAAAAATCCAGTCACGGGGAGGAAGATTCGCGCAAGTCTGCAGCTGCTGCAGTGGCAGCAAAGCTGACCGCATCAACATCCTCGGCCCAGGTGCTCTCTTATGTCCTCTCCTCTCTGGCATCCGAGGGTGTCATCAGCAATCCCATTAACGAGTCTTCTGGTGATTATCCATCCGAAAAGAGGCTTAAGCTGGAGCATGACCAATCGTCGTCCTATTTACCCCGGAGCCCTAACCCGTCTGCCTCTCCTCCGTTCTCGCATTCTGAATCTCACCCGCACAATGTCCCTACCACCAACCAACAATACACTCCCACTCCCAGTGAGGCACCACCTCCCCCATCATCTTCTCCTCCACCACTTCCACCATTACCACCCCAACCACAGTACCAAGTGCCCCAATATGTGCCGTCTCCTGGACAATACACCTACAACGTGGCCCAACAACAGCCACCCCCAATGCCTAGTTATCAAGCAGGAGCTCCAGTAAATGGCGTTTCTCCCTTCATGACACCGGCCAATTCTTATCAGAGTTTTCAAGGTTCAGATGGTAAACTCTACAACCAGCAATCACCGGTGCAAATGACGCCGATTTCGAGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTD_bind

Protein Analysis

519

Amino Acids

56.99

Weight (kDa)

6.25

Isoelectric Point (pI)

60.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CID PF04818 9 - 120 5.7e-36 CID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 931
AccB1I GGYRCC 3 cut(s) 434, 578, 1279
AccB7I CCANNNNNTGG 2 cut(s) 172, 1442
AccII CGCG 2 cut(s) 162, 985
AciI CCGC 2 cut(s) 1023, 1229
AcoI YGGCCR 4 cut(s) 364, 499, 795, 1467
AcsI RAATTY 3 cut(s) 230, 242, 296
AcuI CTGAAG 2 cut(s) 710, 752
AcyI GRCGYC 1 cut(s) 1538
AfaI GTAC 3 cut(s) 740, 932, 1340
AfiI CCNNNNNNNGG 6 cut(s) 172, 769, 969, 1134, 1367, 1442
AflII CTTAAG 1 cut(s) 1139
AgeI ACCGGT 1 cut(s) 1525
AgsI TTSAA 6 cut(s) 16, 128, 589, 665, 683, 1490
AhdI GACNNNNNGTC 1 cut(s) 1159
AjnI CCWGG 2 cut(s) 1041, 1366
AleI CACNNNNGTG 1 cut(s) 88
Alw21I GWGCWC 4 cut(s) 157, 860, 1050, 1435
Alw26I GTCTC 3 cut(s) 68, 711, 1368
AlwNI CAGNNNCTG 1 cut(s) 1001
AoxI GGCC 6 cut(s) 364, 499, 795, 1038, 1389, 1467
ApeKI GCWGC 5 cut(s) 311, 995, 998, 1001, 1010
ApoI RAATTY 3 cut(s) 230, 242, 296
ArsI GACNNNNNNTTYG 1 cut(s) 1528
AsiGI ACCGGT 1 cut(s) 1525
Asp700I GAANNNNTTC 1 cut(s) 223
AspLEI GCGC 1 cut(s) 987
AspS9I GGNCC 2 cut(s) 1039, 1390
AsuC2I CCSGG 2 cut(s) 401, 1177
AsuHPI GGTGA 4 cut(s) 251, 1127, 1217, 1515
AsuNHI GCTAGC 1 cut(s) 40
BaeGI GKGCMC 1 cut(s) 1350
BaeI ACNNNNGTAYC 2 cut(s) 1322, 1355
BalI TGGCCA 2 cut(s) 501, 797
BanI GGYRCC 3 cut(s) 434, 578, 1279
BanII GRGCYC 3 cut(s) 860, 1184, 1435
BbsI GAAGAC 1 cut(s) 1101
Bbv12I GWGCWC 4 cut(s) 157, 860, 1050, 1435
BbvI GCAGC 5 cut(s) 323, 985, 988, 1007, 1022
BccI CCATC 5 cut(s) 499, 819, 1132, 1300, 1493
BceAI ACGGC 2 cut(s) 379, 1345
BciT130I CCWGG 2 cut(s) 1043, 1368
BcnI CCSGG 2 cut(s) 401, 1177
BcoDI GTCTC 3 cut(s) 68, 711, 1368
BfaI CTAG 6 cut(s) 41, 138, 492, 554, 746, 1416
BfmI CTRYAG 3 cut(s) 879, 993, 1002
BfrI CTTAAG 1 cut(s) 1139
BfuAI ACCTGC 1 cut(s) 931
BisI GCNGC 5 cut(s) 312, 996, 999, 1002, 1011
BlsI GCNGC 5 cut(s) 313, 997, 1000, 1003, 1012
Bme1390I CCNGG 4 cut(s) 401, 1043, 1177, 1368
BmeRI GACNNNNNGTC 1 cut(s) 1159
BmgT120I GGNCC 2 cut(s) 1039, 1390
BmiI GGNNCC 4 cut(s) 436, 580, 1181, 1281
BmrFI CCNGG 4 cut(s) 401, 1043, 1177, 1368
BmrI ACTGGG 2 cut(s) 778, 1266
BmsI GCATC 5 cut(s) 253, 454, 763, 1034, 1082
BmtI GCTAGC 1 cut(s) 44
BmuI ACTGGG 2 cut(s) 778, 1266
BpiI GAAGAC 1 cut(s) 1101
BpmI CTGGAG 3 cut(s) 906, 1166, 1419
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 2 cut(s) 401, 1177
BsaHI GRCGYC 1 cut(s) 1538
BsaJI CCNNGG 4 cut(s) 1035, 1041, 1077, 1175
BsaWI WCCGGW 1 cut(s) 1525
BsaXI ACNNNNNCTCC 6 cut(s) 722, 752, 1252, 1282, 1422, 1452
Bsc4I CCNNNNNNNGG 6 cut(s) 172, 769, 969, 1134, 1367, 1442
Bse118I RCCGGY 2 cut(s) 1465, 1525
Bse1I ACTGG 5 cut(s) 784, 923, 963, 1272, 1436
BseBI CCWGG 2 cut(s) 1043, 1368
BseDI CCNNGG 4 cut(s) 1035, 1041, 1077, 1175
BseGI GGATG 7 cut(s) 343, 445, 514, 778, 1031, 1073, 1124
BseLI CCNNNNNNNGG 6 cut(s) 172, 769, 969, 1134, 1367, 1442
BseMII CTCAG 1 cut(s) 606
BseNI ACTGG 5 cut(s) 784, 923, 963, 1272, 1436
BseRI GAGGAG 4 cut(s) 638, 1054, 1191, 1293
BseSI GKGCMC 1 cut(s) 1350
BseXI GCAGC 5 cut(s) 323, 985, 988, 1007, 1022
Bsh1236I CGCG 2 cut(s) 162, 985
BshFI GGCC 6 cut(s) 366, 501, 797, 1040, 1391, 1469
BshNI GGYRCC 3 cut(s) 434, 578, 1279
BshTI ACCGGT 1 cut(s) 1525
BsiHKAI GWGCWC 4 cut(s) 157, 860, 1050, 1435
BsiSI CCGG 4 cut(s) 401, 1177, 1466, 1526
BslFI GGGAC 2 cut(s) 766, 1223
BslI CCNNNNNNNGG 6 cut(s) 172, 769, 969, 1134, 1367, 1442
BsmAI GTCTC 3 cut(s) 68, 711, 1368
BsmBI CGTCTC 1 cut(s) 1368
BsmFI GGGAC 2 cut(s) 766, 1223
BsmI GAATGC 3 cut(s) 427, 646, 1213
BsnI GGCC 6 cut(s) 366, 501, 797, 1040, 1391, 1469
Bsp1286I GDGCHC 6 cut(s) 157, 860, 1050, 1184, 1350, 1435
Bsp1407I TGTACA 1 cut(s) 930
Bsp143I GATC 1 cut(s) 748
BspACI CCGC 2 cut(s) 1023, 1229
BspANI GGCC 6 cut(s) 366, 501, 797, 1040, 1391, 1469
BspCNI CTCAG 1 cut(s) 605
BspFNI CGCG 2 cut(s) 162, 985
BspHI TCATGA 1 cut(s) 1458
BspLI GGNNCC 4 cut(s) 436, 580, 1181, 1281
BspMAI CTGCAG 2 cut(s) 997, 1006
BspMI ACCTGC 1 cut(s) 931
BspOI GCTAGC 1 cut(s) 44
BspQI GCTCTTC 2 cut(s) 273, 729
BspT107I GGYRCC 3 cut(s) 434, 578, 1279
BspTI CTTAAG 1 cut(s) 1139
BsrFI RCCGGY 2 cut(s) 1465, 1525
BsrGI TGTACA 1 cut(s) 930
BsrI ACTGG 5 cut(s) 784, 923, 963, 1272, 1436
BssAI RCCGGY 2 cut(s) 1465, 1525
BssECI CCNNGG 4 cut(s) 1035, 1041, 1077, 1175
BssMI GATC 1 cut(s) 748
BssNI GRCGYC 1 cut(s) 1538
Bst2UI CCWGG 2 cut(s) 1043, 1368
Bst4CI ACNGT 2 cut(s) 212, 1338
Bst6I CTCTTC 3 cut(s) 273, 729, 901
BstACI GRCGYC 1 cut(s) 1538
BstAFI CTTAAG 1 cut(s) 1139
BstAPI GCANNNNNTGC 1 cut(s) 1001
BstAUI TGTACA 1 cut(s) 930
BstC8I GCNNGC 4 cut(s) 42, 63, 67, 795
BstDEI CTNAG 4 cut(s) 592, 650, 671, 713
BstF5I GGATG 7 cut(s) 343, 445, 514, 778, 1031, 1073, 1124
BstFNI CGCG 2 cut(s) 162, 985
BstHHI GCGC 1 cut(s) 987
BstKTI GATC 1 cut(s) 751
BstMAI GTCTC 3 cut(s) 68, 711, 1368
BstMBI GATC 1 cut(s) 748
BstMWI GCNNNNNNNGC 6 cut(s) 46, 458, 1001, 1007, 1010, 1537
BstNI CCWGG 2 cut(s) 1043, 1368
BstSCI CCNGG 4 cut(s) 399, 1041, 1175, 1366
BstSFI CTRYAG 3 cut(s) 879, 993, 1002
BstSLI GKGCMC 1 cut(s) 1350
BstUI CGCG 2 cut(s) 162, 985
BstV1I GCAGC 5 cut(s) 323, 985, 988, 1007, 1022
BstV2I GAAGAC 1 cut(s) 1101
BstXI CCANNNNNNTGG 1 cut(s) 898
BsuRI GGCC 6 cut(s) 366, 501, 797, 1040, 1391, 1469
BtsCI GGATG 7 cut(s) 343, 445, 514, 778, 1031, 1073, 1124
BtsI GCAGTG 1 cut(s) 1011
BtsIMutI CAGTG 2 cut(s) 1011, 1279
BveI ACCTGC 1 cut(s) 931
Cac8I GCNNGC 4 cut(s) 42, 63, 67, 795
CaiI CAGNNNCTG 1 cut(s) 1001
CciI TCATGA 1 cut(s) 1458
CfoI GCGC 1 cut(s) 987
Cfr10I RCCGGY 2 cut(s) 1465, 1525
Cfr13I GGNCC 2 cut(s) 1039, 1390
CseI GACGC 1 cut(s) 1546
Csp6I GTAC 3 cut(s) 739, 931, 1339
CspAI ACCGGT 1 cut(s) 1525
CviAII CATG 5 cut(s) 132, 497, 605, 1151, 1459
CviQI GTAC 3 cut(s) 739, 931, 1339
DdeI CTNAG 4 cut(s) 592, 650, 671, 713
DpnI GATC 1 cut(s) 750
DpnII GATC 1 cut(s) 748
DriI GACNNNNNGTC 1 cut(s) 1159
EaeI YGGCCR 4 cut(s) 364, 499, 795, 1467
Eam1104I CTCTTC 3 cut(s) 273, 729, 901
Eam1105I GACNNNNNGTC 1 cut(s) 1159
EarI CTCTTC 3 cut(s) 273, 729, 901
Ecl136II GAGCTC 2 cut(s) 858, 1433
Eco24I GRGCYC 3 cut(s) 860, 1184, 1435
Eco53kI GAGCTC 2 cut(s) 858, 1433
Eco57I CTGAAG 2 cut(s) 710, 752
EcoICRI GAGCTC 2 cut(s) 858, 1433
EcoRII CCWGG 2 cut(s) 1041, 1366
EcoT38I GRGCYC 3 cut(s) 860, 1184, 1435
Esp3I CGTCTC 1 cut(s) 1368
FaeI CATG 5 cut(s) 135, 500, 608, 1154, 1462
FaqI GGGAC 2 cut(s) 766, 1223
FatI CATG 5 cut(s) 131, 496, 604, 1150, 1458
FauI CCCGC 1 cut(s) 1236
FauNDI CATATG 1 cut(s) 102
Fnu4HI GCNGC 5 cut(s) 312, 996, 999, 1002, 1011
FokI GGATG 7 cut(s) 350, 432, 521, 785, 1018, 1060, 1111
FriOI GRGCYC 3 cut(s) 860, 1184, 1435
Fsp4HI GCNGC 5 cut(s) 312, 996, 999, 1002, 1011
FspBI CTAG 6 cut(s) 41, 138, 492, 554, 746, 1416
GlaI GCGC 1 cut(s) 986
GluI GCNGC 5 cut(s) 312, 996, 999, 1002, 1011
GsuI CTGGAG 3 cut(s) 906, 1166, 1419
HaeIII GGCC 6 cut(s) 366, 501, 797, 1040, 1391, 1469
HapII CCGG 4 cut(s) 401, 1177, 1466, 1526
HgaI GACGC 1 cut(s) 1546
HhaI GCGC 1 cut(s) 987
Hin1I GRCGYC 1 cut(s) 1538
Hin1II CATG 5 cut(s) 135, 500, 608, 1154, 1462
Hin6I GCGC 1 cut(s) 985
HinP1I GCGC 1 cut(s) 985
HinfI GANTC 6 cut(s) 370, 683, 779, 980, 1106, 1220
HpaII CCGG 4 cut(s) 401, 1177, 1466, 1526
HphI GGTGA 4 cut(s) 251, 1127, 1217, 1515
Hpy166II GTNNAC 3 cut(s) 359, 933, 1505
Hpy188III TCNNGA 8 cut(s) 260, 272, 554, 690, 728, 770, 853, 1459
Hpy8I GTNNAC 3 cut(s) 359, 933, 1505
Hpy99I CGWCG 1 cut(s) 1165
HpyAV CCTTC 3 cut(s) 244, 897, 1465
HpyCH4III ACNGT 2 cut(s) 212, 1338
HpyCH4IV ACGT 1 cut(s) 1386
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 458, 1001, 1007, 1010, 1537
HpyF3I CTNAG 4 cut(s) 592, 650, 671, 713
HpySE526I ACGT 1 cut(s) 1386
Hsp92I GRCGYC 1 cut(s) 1538
Hsp92II CATG 5 cut(s) 135, 500, 608, 1154, 1462
HspAI GCGC 1 cut(s) 985
Kzo9I GATC 1 cut(s) 748
LguI GCTCTTC 2 cut(s) 273, 729
LmnI GCTCC 7 cut(s) 160, 730, 925, 1147, 1179, 1430, 1438
Lsp1109I GCAGC 5 cut(s) 323, 985, 988, 1007, 1022
LweI GCATC 5 cut(s) 253, 454, 763, 1034, 1082
MaeI CTAG 6 cut(s) 41, 138, 492, 554, 746, 1416
MaeII ACGT 1 cut(s) 1386
MaeIII GTNAC 2 cut(s) 410, 965
MalI GATC 1 cut(s) 750
MboI GATC 1 cut(s) 748
MboII GAAGA 8 cut(s) 248, 260, 315, 716, 918, 989, 1101, 1290
MhlI GDGCHC 6 cut(s) 157, 860, 1050, 1184, 1350, 1435
MlsI TGGCCA 2 cut(s) 501, 797
MluNI TGGCCA 2 cut(s) 501, 797
MlyI GAGTC 2 cut(s) 788, 1115
MmeI TCCRAC 2 cut(s) 370, 444
Mox20I TGGCCA 2 cut(s) 501, 797
MroXI GAANNNNTTC 1 cut(s) 223
MscI TGGCCA 2 cut(s) 501, 797
MseI TTAA 5 cut(s) 282, 327, 818, 1101, 1140
MslI CAYNNNNRTG 2 cut(s) 88, 432
Msp20I TGGCCA 2 cut(s) 501, 797
MspA1I CMGCKG 1 cut(s) 998
MspCI CTTAAG 1 cut(s) 1139
MspI CCGG 4 cut(s) 401, 1177, 1466, 1526
MspR9I CCNGG 4 cut(s) 401, 1043, 1177, 1368
Mva1269I GAATGC 3 cut(s) 427, 646, 1213
MvaI CCWGG 2 cut(s) 1043, 1368
MvnI CGCG 2 cut(s) 162, 985
MwoI GCNNNNNNNGC 6 cut(s) 46, 458, 1001, 1007, 1010, 1537
NciI CCSGG 2 cut(s) 401, 1177
NdeI CATATG 1 cut(s) 102
NdeII GATC 1 cut(s) 748
NheI GCTAGC 1 cut(s) 40
NlaIII CATG 5 cut(s) 135, 500, 608, 1154, 1462
NlaIV GGNNCC 4 cut(s) 436, 580, 1181, 1281
NmeAIII GCCGAG 1 cut(s) 1016
NmuCI GTSAC 1 cut(s) 965
OliI CACNNNNGTG 1 cut(s) 88
PagI TCATGA 1 cut(s) 1458
PciSI GCTCTTC 2 cut(s) 273, 729
PcsI WCGNNNNNNNCGW 1 cut(s) 1544
PctI GAATGC 3 cut(s) 427, 646, 1213
PdmI GAANNNNTTC 1 cut(s) 223
PfeI GAWTC 4 cut(s) 370, 683, 980, 1220
PflMI CCANNNNNTGG 2 cut(s) 172, 1442
PfoI TCCNGGA 1 cut(s) 1366
PinAI ACCGGT 1 cut(s) 1525
PkrI GCNGC 5 cut(s) 313, 997, 1000, 1003, 1012
PleI GAGTC 2 cut(s) 787, 1114
PpsI GAGTC 2 cut(s) 787, 1114
Psp124BI GAGCTC 2 cut(s) 860, 1435
Psp6I CCWGG 2 cut(s) 1041, 1366
PspGI CCWGG 2 cut(s) 1041, 1366
PspN4I GGNNCC 4 cut(s) 436, 580, 1181, 1281
PspPI GGNCC 2 cut(s) 1039, 1390
PstI CTGCAG 2 cut(s) 997, 1006
PstNI CAGNNNCTG 1 cut(s) 1001
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 3 cut(s) 740, 932, 1340
RsaNI GTAC 3 cut(s) 739, 931, 1339
RseI CAYNNNNRTG 2 cut(s) 88, 432
SacI GAGCTC 2 cut(s) 860, 1435
SapI GCTCTTC 2 cut(s) 273, 729
SaqAI TTAA 5 cut(s) 282, 327, 818, 1101, 1140
SatI GCNGC 5 cut(s) 312, 996, 999, 1002, 1011
Sau3AI GATC 1 cut(s) 748
Sau96I GGNCC 2 cut(s) 1039, 1390
SchI GAGTC 2 cut(s) 788, 1115
ScrFI CCNGG 4 cut(s) 401, 1043, 1177, 1368
SduI GDGCHC 6 cut(s) 157, 860, 1050, 1184, 1350, 1435
SfaNI GCATC 5 cut(s) 253, 454, 763, 1034, 1082
SfcI CTRYAG 3 cut(s) 879, 993, 1002
SgrAI CRCCGGYG 1 cut(s) 1525
SmiMI CAYNNNNRTG 2 cut(s) 88, 432
SmlI CTYRAG 2 cut(s) 374, 1139
SmoI CTYRAG 2 cut(s) 374, 1139
SsiI CCGC 2 cut(s) 1023, 1229
SspMI CTAG 6 cut(s) 41, 138, 492, 554, 746, 1416
SstI GAGCTC 2 cut(s) 860, 1435
StyD4I CCNGG 4 cut(s) 399, 1041, 1175, 1366
TaaI ACNGT 2 cut(s) 212, 1338
TaiI ACGT 1 cut(s) 1389
TaqI TCGA 1 cut(s) 1547
TatI WGTACW 2 cut(s) 738, 930
TfiI GAWTC 4 cut(s) 370, 683, 980, 1220
Tru1I TTAA 5 cut(s) 282, 327, 818, 1101, 1140
Tru9I TTAA 5 cut(s) 282, 327, 818, 1101, 1140
TscAI CASTG 2 cut(s) 1011, 1279
TseFI GTSAC 1 cut(s) 965
TseI GCWGC 5 cut(s) 311, 995, 998, 1001, 1010
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 6 cut(s) 119, 137, 309, 593, 836, 1447
TspGWI ACGGA 1 cut(s) 1194
TspRI CASTG 2 cut(s) 1011, 1279
Van91I CCANNNNNTGG 2 cut(s) 172, 1442
Vha464I CTTAAG 1 cut(s) 1139
XapI RAATTY 3 cut(s) 230, 242, 296
XbaI TCTAGA 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
XmnI GAANNNNTTC 1 cut(s) 223
XspI CTAG 6 cut(s) 41, 138, 492, 554, 746, 1416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.