Rroxscaffold_2G00076820

Regulation of nuclear pre-mRNA domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010397
Physical Location & Seq
Reverse (-)
534512 .. 539627
5116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00076820.1

Sequence Viewer

Length: 1563 bp
ATGGGAAGTGCATTCAATCCTCAGATTTTAGTGGAGAAGCTAGCCAAGCTCAACAGCTCCCAAGCGAGCATAGAGACTTTATCACATTGGTGCATTTTCCACATGAATAAAGCAAGACAAGTTGTTGAAACTTGGGCCAGGCAATTTCATTCTTCTCCACGTGAACAAAGATTGGCTTTTCTGTATCTTGCAAATGATATTTTACAGAACAGTAGGCGAAAAGGTGCAGAGTTTGTTGGTGAGTTCTGGAAGGTACTTCCAGACGCTCTTCGCGATGTAATTGAGAGTGGAGATGAAGTTGGCAGAAATGCGGCACAACGACTGATTAATATATGGGAAGAAAGAAAAGTGTTTGGTTCTCGTGGCCAAATTCTAAAGGAAGAGTTTGTGGGAAGGCAGTTGGAAAGTAATAATAGAAATGGGAAGCATTTATTGCCTGCTGGAACTTCATTGGACAAGATAGTATCAGATTACCAAGCTATTTATGGCCAAATGGATGAAGACACTATCTTGAGAAAATGTAAGAATGCTATCAACTGTCTTGAGAAAGCAGACAAGGAGGTTGGTGGTGATTTCAATTCAGGGCAATTTCAAGGAGATGCATTTGCGCAGGAGGTACATGGGCAGCATGCCATACTAAGGGACTGTATTGATCAACTAACTGCAGTGGAATCATCAAGAGCTACTCTTGTGTCTCATCTGAAAGAGGCTCTTCAGGAGCAGGAATTCAAGTTGGATCAACTCCGAAACCAACTTCAGGCGGCACAGTCACAGTCAGAACAAGCCAGTAATTTCTGCCGTCAGTTAATGACTAGCAGCAATAATTTGCAGCCGATAGACCAGAACTCAAAGGAAGCTCATACCTCTGCAGCACCTCACAGCTTTATGGCAGTGGGTAGAGAGCAATCAGCTCCAGTAATGTACACACAGCAGGTTCCTTTTACCGAAAAGTCTGGTCATGTGGAGGAAGATCCTCGCAAGTCTGCAGCTGCTGCAGTGGCAGCAAAGCTAACTGCATCAACGTCCTCAGCCCAGATGCTCTCTTATGTCCTCTCCTCTCTGGCATCAGAGGGTGTCATCAGCAATCCAATTAAAGGGTCTTCTGGTGATTATCCATCTGAAAAGAGACTTAAACTCGATAATGATAACCAATTGTTACCTTATGTTCCCCAGAATCCTAACCCATCTGCATCTTCTCCCTTCTCACATTCTGAATCACTCCAGCTGAATGTGTCTACCACCAGCCAACAATATACTCCTACCTTGCCGCCACTTCCCCCATCATCTCCTCCACCATTGCCACCGTTACCACCAGTATCACAGTACCCAGTGTCCCAGTATTTGCAGGCTCCTGGATCAACAACTTCTGTATCTTTTAGTTATAGCATGGCCCAGCCACAGACTTCCGCAGTGCCTGGTTATACACCAGTGGGAGGAGCTCAAGTGAATGGGCTTCCACCTTTGATGGCACCAGCAAATCCTTATCAGAGTTTTCAGGGTTCAGATGGTAAATATTACAACCAACAATCATCGATGCAATTGGCGCCAATGTCTAGACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTD_bind

Protein Analysis

520

Amino Acids

57.1

Weight (kDa)

6.16

Isoelectric Point (pI)

54.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CID PF04818 9 - 120 3.9e-37 CID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 609
Acc36I ACCTGC 1 cut(s) 922
AccB1I GGYRCC 2 cut(s) 1468, 1543
AccI GTMKAC 1 cut(s) 1235
AccII CGCG 1 cut(s) 273
AciI CCGC 4 cut(s) 311, 761, 1268, 1407
AclWI GGATC 3 cut(s) 744, 965, 1363
AcoI YGGCCR 2 cut(s) 364, 487
AcsI RAATTY 2 cut(s) 369, 725
AcuI CTGAAG 2 cut(s) 698, 740
AcvI CACGTG 1 cut(s) 161
AcyI GRCGYC 1 cut(s) 1544
AfaI GTAC 4 cut(s) 255, 618, 923, 1325
AfiI CCNNNNNNNGG 4 cut(s) 639, 757, 1094, 1433
AgsI TTSAA 5 cut(s) 16, 128, 577, 593, 730
AjnI CCWGG 3 cut(s) 137, 1351, 1414
AleI CACNNNNGTG 1 cut(s) 88
Alw21I GWGCWC 1 cut(s) 1441
Alw26I GTCTC 3 cut(s) 68, 699, 1120
AlwI GGATC 3 cut(s) 744, 965, 1363
AlwNI CAGNNNCTG 2 cut(s) 992, 1415
AoxI GGCC 4 cut(s) 135, 364, 487, 1389
ApeKI GCWGC 8 cut(s) 625, 816, 829, 869, 986, 989, 992, 1001
ApoI RAATTY 2 cut(s) 369, 725
AseI ATTAAT 1 cut(s) 327
AspLEI GCGC 2 cut(s) 610, 1546
AspS9I GGNCC 2 cut(s) 135, 1390
AsuHPI GGTGA 3 cut(s) 251, 581, 1118
AsuNHI GCTAGC 1 cut(s) 40
BaeI ACNNNNGTAYC 2 cut(s) 1353, 1386
BalI TGGCCA 2 cut(s) 366, 489
BanI GGYRCC 2 cut(s) 1468, 1543
BanII GRGCYC 1 cut(s) 1441
BauI CACGAG 1 cut(s) 360
BbrPI CACGTG 1 cut(s) 161
BbsI GAAGAC 2 cut(s) 507, 1092
Bbv12I GWGCWC 1 cut(s) 1441
BbvCI CCTCAGC 1 cut(s) 1027
BbvI GCAGC 8 cut(s) 637, 828, 841, 881, 976, 979, 998, 1013
BccI CCATC 5 cut(s) 1123, 1192, 1288, 1459, 1499
BceAI ACGGC 1 cut(s) 783
BciT130I CCWGG 3 cut(s) 139, 1353, 1416
BclI TGATCA 1 cut(s) 652
BcoDI GTCTC 3 cut(s) 68, 699, 1120
BfaI CTAG 3 cut(s) 41, 813, 1554
BfmI CTRYAG 4 cut(s) 663, 867, 984, 993
BfoI RGCGCY 1 cut(s) 1547
BfuAI ACCTGC 1 cut(s) 922
Bme1390I CCNGG 3 cut(s) 139, 1353, 1416
BmgT120I GGNCC 2 cut(s) 135, 1390
BmiI GGNNCC 4 cut(s) 936, 1350, 1470, 1545
BmrFI CCNGG 3 cut(s) 139, 1353, 1416
BmrI ACTGGG 2 cut(s) 1322, 1330
BmsI GCATC 6 cut(s) 589, 1025, 1026, 1073, 1199, 1524
BmtI GCTAGC 1 cut(s) 44
BmuI ACTGGG 2 cut(s) 1322, 1330
BpiI GAAGAC 2 cut(s) 507, 1092
BpmI CTGGAG 2 cut(s) 897, 1205
Bpu10I CCTNAGC 1 cut(s) 1027
BpuEI CTTGAG 3 cut(s) 532, 563, 1425
Bsa29I ATCGAT 1 cut(s) 1532
BsaAI YACGTR 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 1544
BsaXI ACNNNNNCTCC 4 cut(s) 282, 312, 1428, 1458
Bsc4I CCNNNNNNNGG 4 cut(s) 639, 757, 1094, 1433
Bse1I ACTGG 6 cut(s) 786, 914, 1313, 1328, 1336, 1427
Bse3DI GCAATG 1 cut(s) 1295
BseBI CCWGG 3 cut(s) 139, 1353, 1416
BseCI ATCGAT 1 cut(s) 1532
BseGI GGATG 1 cut(s) 502
BseLI CCNNNNNNNGG 4 cut(s) 639, 757, 1094, 1433
BseMI GCAATG 1 cut(s) 1295
BseMII CTCAG 2 cut(s) 35, 1041
BseNI ACTGG 6 cut(s) 786, 914, 1313, 1328, 1336, 1427
BseRI GAGGAG 3 cut(s) 1045, 1278, 1449
BseXI GCAGC 8 cut(s) 637, 828, 841, 881, 976, 979, 998, 1013
BseYI CCCAGC 1 cut(s) 1392
BsgI GTGCAG 1 cut(s) 246
Bsh1236I CGCG 1 cut(s) 273
BshFI GGCC 4 cut(s) 137, 366, 489, 1391
BshNI GGYRCC 2 cut(s) 1468, 1543
BshVI ATCGAT 1 cut(s) 1532
BsiHKAI GWGCWC 1 cut(s) 1441
BslFI GGGAC 2 cut(s) 656, 1318
BslI CCNNNNNNNGG 4 cut(s) 639, 757, 1094, 1433
BsmAI GTCTC 3 cut(s) 68, 699, 1120
BsmFI GGGAC 2 cut(s) 656, 1318
BsmI GAATGC 2 cut(s) 11, 532
BsnI GGCC 4 cut(s) 137, 366, 489, 1391
Bsp1286I GDGCHC 1 cut(s) 1441
Bsp1407I TGTACA 1 cut(s) 921
Bsp143I GATC 4 cut(s) 652, 736, 970, 1355
Bsp68I TCGCGA 1 cut(s) 273
BspACI CCGC 4 cut(s) 311, 761, 1268, 1407
BspANI GGCC 4 cut(s) 137, 366, 489, 1391
BspCNI CTCAG 2 cut(s) 34, 1040
BspDI ATCGAT 1 cut(s) 1532
BspFNI CGCG 1 cut(s) 273
BspLI GGNNCC 4 cut(s) 936, 1350, 1470, 1545
BspMAI CTGCAG 4 cut(s) 667, 871, 988, 997
BspMI ACCTGC 1 cut(s) 922
BspOI GCTAGC 1 cut(s) 44
BspPI GGATC 3 cut(s) 744, 965, 1363
BspQI GCTCTTC 2 cut(s) 273, 717
BspT107I GGYRCC 2 cut(s) 1468, 1543
BsrDI GCAATG 1 cut(s) 1295
BsrGI TGTACA 1 cut(s) 921
BsrI ACTGG 6 cut(s) 786, 914, 1313, 1328, 1336, 1427
BssMI GATC 4 cut(s) 652, 736, 970, 1355
BssNI GRCGYC 1 cut(s) 1544
BssSI CACGAG 1 cut(s) 360
Bst2BI CACGAG 1 cut(s) 360
Bst2UI CCWGG 3 cut(s) 139, 1353, 1416
Bst4CI ACNGT 7 cut(s) 212, 539, 647, 768, 774, 1305, 1323
Bst6I CTCTTC 3 cut(s) 273, 375, 717
BstACI GRCGYC 1 cut(s) 1544
BstAPI GCANNNNNTGC 2 cut(s) 433, 992
BstAUI TGTACA 1 cut(s) 921
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 5 cut(s) 42, 67, 438, 630, 1347
BstDEI CTNAG 3 cut(s) 21, 638, 1027
BstF5I GGATG 1 cut(s) 502
BstFNI CGCG 1 cut(s) 273
BstH2I RGCGCY 1 cut(s) 1547
BstHHI GCGC 2 cut(s) 610, 1546
BstKTI GATC 4 cut(s) 655, 739, 973, 1358
BstMAI GTCTC 3 cut(s) 68, 699, 1120
BstMBI GATC 4 cut(s) 652, 736, 970, 1355
BstMWI GCNNNNNNNGC 6 cut(s) 46, 433, 992, 998, 1001, 1543
BstNI CCWGG 3 cut(s) 139, 1353, 1416
BstNSI RCATGY 1 cut(s) 632
BstSCI CCNGG 3 cut(s) 137, 1351, 1414
BstSFI CTRYAG 4 cut(s) 663, 867, 984, 993
BstUI CGCG 1 cut(s) 273
BstV1I GCAGC 8 cut(s) 637, 828, 841, 881, 976, 979, 998, 1013
BstV2I GAAGAC 2 cut(s) 507, 1092
BstX2I RGATCY 1 cut(s) 970
BstYI RGATCY 1 cut(s) 970
Bsu15I ATCGAT 1 cut(s) 1532
BsuRI GGCC 4 cut(s) 137, 366, 489, 1391
BsuTUI ATCGAT 1 cut(s) 1532
BtgZI GCGATG 1 cut(s) 288
BtsCI GGATG 1 cut(s) 502
BtsI GCAGTG 4 cut(s) 672, 897, 1002, 1416
BtsIMutI CAGTG 6 cut(s) 672, 897, 1002, 1335, 1416, 1434
BtuMI TCGCGA 1 cut(s) 273
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 5 cut(s) 42, 67, 438, 630, 1347
CaiI CAGNNNCTG 2 cut(s) 992, 1415
CfoI GCGC 2 cut(s) 610, 1546
Cfr13I GGNCC 2 cut(s) 135, 1390
ClaI ATCGAT 1 cut(s) 1532
CseI GACGC 1 cut(s) 272
Csp6I GTAC 4 cut(s) 254, 617, 922, 1324
CviAII CATG 5 cut(s) 103, 620, 629, 959, 1387
CviQI GTAC 4 cut(s) 254, 617, 922, 1324
DdeI CTNAG 3 cut(s) 21, 638, 1027
DinI GGCGCC 1 cut(s) 1545
DpnI GATC 4 cut(s) 654, 738, 972, 1357
DpnII GATC 4 cut(s) 652, 736, 970, 1355
EaeI YGGCCR 2 cut(s) 364, 487
Eam1104I CTCTTC 3 cut(s) 273, 375, 717
EarI CTCTTC 3 cut(s) 273, 375, 717
Ecl136II GAGCTC 1 cut(s) 1439
Eco24I GRGCYC 1 cut(s) 1441
Eco53kI GAGCTC 1 cut(s) 1439
Eco57I CTGAAG 2 cut(s) 698, 740
Eco72I CACGTG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 1439
EcoRI GAATTC 1 cut(s) 725
EcoRII CCWGG 3 cut(s) 137, 1351, 1414
EcoT22I ATGCAT 1 cut(s) 604
EcoT38I GRGCYC 1 cut(s) 1441
EgeI GGCGCC 1 cut(s) 1545
EheI GGCGCC 1 cut(s) 1545
FaeI CATG 5 cut(s) 106, 623, 632, 962, 1390
FalI AAGNNNNNCTT 4 cut(s) 160, 192, 696, 728
FaqI GGGAC 2 cut(s) 656, 1318
FatI CATG 5 cut(s) 102, 619, 628, 958, 1386
FbaI TGATCA 1 cut(s) 652
FblI GTMKAC 1 cut(s) 1235
FokI GGATG 1 cut(s) 509
FriOI GRGCYC 1 cut(s) 1441
FspBI CTAG 3 cut(s) 41, 813, 1554
FspI TGCGCA 1 cut(s) 609
GlaI GCGC 2 cut(s) 609, 1545
GsaI CCCAGC 1 cut(s) 1396
GsuI CTGGAG 2 cut(s) 897, 1205
HaeII RGCGCY 1 cut(s) 1547
HaeIII GGCC 4 cut(s) 137, 366, 489, 1391
HgaI GACGC 1 cut(s) 272
HhaI GCGC 2 cut(s) 610, 1546
Hin1I GRCGYC 1 cut(s) 1544
Hin1II CATG 5 cut(s) 106, 623, 632, 962, 1390
Hin6I GCGC 2 cut(s) 608, 1544
HinP1I GCGC 2 cut(s) 608, 1544
HinfI GANTC 3 cut(s) 671, 1174, 1214
HphI GGTGA 3 cut(s) 251, 581, 1118
Hpy166II GTNNAC 3 cut(s) 164, 924, 1236
Hpy188III TCNNGA 8 cut(s) 247, 260, 272, 511, 542, 678, 716, 1554
Hpy8I GTNNAC 3 cut(s) 164, 924, 1236
HpyAV CCTTC 3 cut(s) 244, 387, 1210
HpyCH4III ACNGT 7 cut(s) 212, 539, 647, 768, 774, 1305, 1323
HpyCH4IV ACGT 2 cut(s) 160, 1022
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 433, 992, 998, 1001, 1543
HpyF3I CTNAG 3 cut(s) 21, 638, 1027
HpySE526I ACGT 2 cut(s) 160, 1022
Hsp92I GRCGYC 1 cut(s) 1544
Hsp92II CATG 5 cut(s) 106, 623, 632, 962, 1390
HspAI GCGC 2 cut(s) 608, 1544
KasI GGCGCC 1 cut(s) 1543
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 4 cut(s) 652, 736, 970, 1355
LguI GCTCTTC 2 cut(s) 273, 717
LmnI GCTCC 5 cut(s) 62, 718, 916, 1354, 1436
Lsp1109I GCAGC 8 cut(s) 637, 828, 841, 881, 976, 979, 998, 1013
LweI GCATC 6 cut(s) 589, 1025, 1026, 1073, 1199, 1524
MaeI CTAG 3 cut(s) 41, 813, 1554
MaeII ACGT 2 cut(s) 160, 1022
MaeIII GTNAC 3 cut(s) 768, 1155, 1305
MalI GATC 4 cut(s) 654, 738, 972, 1357
MboI GATC 4 cut(s) 652, 736, 970, 1355
MboII GAAGA 9 cut(s) 144, 260, 350, 392, 512, 704, 980, 1092, 1185
MfeI CAATTG 2 cut(s) 1151, 1538
MflI RGATCY 1 cut(s) 970
MhlI GDGCHC 1 cut(s) 1441
MlsI TGGCCA 2 cut(s) 366, 489
MluNI TGGCCA 2 cut(s) 366, 489
Mly113I GGCGCC 1 cut(s) 1544
MmeI TCCRAC 2 cut(s) 381, 714
Mox20I TGGCCA 2 cut(s) 366, 489
Mph1103I ATGCAT 1 cut(s) 604
MscI TGGCCA 2 cut(s) 366, 489
MseI TTAA 4 cut(s) 327, 806, 1092, 1131
MslI CAYNNNNRTG 1 cut(s) 88
Msp20I TGGCCA 2 cut(s) 366, 489
MspA1I CMGCKG 2 cut(s) 989, 1225
MspR9I CCNGG 3 cut(s) 139, 1353, 1416
MunI CAATTG 2 cut(s) 1151, 1538
Mva1269I GAATGC 2 cut(s) 11, 532
MvaI CCWGG 3 cut(s) 139, 1353, 1416
MvnI CGCG 1 cut(s) 273
MwoI GCNNNNNNNGC 6 cut(s) 46, 433, 992, 998, 1001, 1543
NarI GGCGCC 1 cut(s) 1544
NdeII GATC 4 cut(s) 652, 736, 970, 1355
NheI GCTAGC 1 cut(s) 40
NlaIII CATG 5 cut(s) 106, 623, 632, 962, 1390
NlaIV GGNNCC 4 cut(s) 936, 1350, 1470, 1545
NmuCI GTSAC 1 cut(s) 768
NruI TCGCGA 1 cut(s) 273
NsbI TGCGCA 1 cut(s) 609
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 1 cut(s) 632
OliI CACNNNNGTG 1 cut(s) 88
PaeI GCATGC 1 cut(s) 632
PciSI GCTCTTC 2 cut(s) 273, 717
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 2 cut(s) 11, 532
PfeI GAWTC 3 cut(s) 671, 1174, 1214
PfoI TCCNGGA 1 cut(s) 1351
PluTI GGCGCC 1 cut(s) 1547
PmaCI CACGTG 1 cut(s) 161
PmlI CACGTG 1 cut(s) 161
Ppu21I YACGTR 1 cut(s) 161
PshBI ATTAAT 1 cut(s) 327
Psp124BI GAGCTC 1 cut(s) 1441
Psp6I CCWGG 3 cut(s) 137, 1351, 1414
PspCI CACGTG 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 1392
PspGI CCWGG 3 cut(s) 137, 1351, 1414
PspN4I GGNNCC 4 cut(s) 936, 1350, 1470, 1545
PspPI GGNCC 2 cut(s) 135, 1390
PstI CTGCAG 4 cut(s) 667, 871, 988, 997
PstNI CAGNNNCTG 2 cut(s) 992, 1415
PsuI RGATCY 1 cut(s) 970
PvuII CAGCTG 2 cut(s) 989, 1225
RruI TCGCGA 1 cut(s) 273
RsaI GTAC 4 cut(s) 255, 618, 923, 1325
RsaNI GTAC 4 cut(s) 254, 617, 922, 1324
RseI CAYNNNNRTG 1 cut(s) 88
SacI GAGCTC 1 cut(s) 1441
SapI GCTCTTC 2 cut(s) 273, 717
SaqAI TTAA 4 cut(s) 327, 806, 1092, 1131
Sau3AI GATC 4 cut(s) 652, 736, 970, 1355
Sau96I GGNCC 2 cut(s) 135, 1390
ScrFI CCNGG 3 cut(s) 139, 1353, 1416
SduI GDGCHC 1 cut(s) 1441
SfaNI GCATC 6 cut(s) 589, 1025, 1026, 1073, 1199, 1524
SfcI CTRYAG 4 cut(s) 663, 867, 984, 993
SfoI GGCGCC 1 cut(s) 1545
SmiMI CAYNNNNRTG 1 cut(s) 88
SmlI CTYRAG 3 cut(s) 511, 542, 1440
SmoI CTYRAG 3 cut(s) 511, 542, 1440
SphI GCATGC 1 cut(s) 632
SsiI CCGC 4 cut(s) 311, 761, 1268, 1407
SspDI GGCGCC 1 cut(s) 1543
SspI AATATT 1 cut(s) 1514
SspMI CTAG 3 cut(s) 41, 813, 1554
SstI GAGCTC 1 cut(s) 1441
StyD4I CCNGG 3 cut(s) 137, 1351, 1414
TaaI ACNGT 7 cut(s) 212, 539, 647, 768, 774, 1305, 1323
TaiI ACGT 2 cut(s) 163, 1025
TaqI TCGA 2 cut(s) 1137, 1532
TatI WGTACW 1 cut(s) 921
TauI GCSGC 3 cut(s) 314, 764, 1270
TfiI GAWTC 3 cut(s) 671, 1174, 1214
Tru1I TTAA 4 cut(s) 327, 806, 1092, 1131
Tru9I TTAA 4 cut(s) 327, 806, 1092, 1131
TscAI CASTG 6 cut(s) 672, 897, 1002, 1335, 1416, 1434
TseFI GTSAC 1 cut(s) 768
TseI GCWGC 8 cut(s) 625, 816, 829, 869, 986, 989, 992, 1001
Tsp45I GTSAC 1 cut(s) 768
TspDTI ATGAA 5 cut(s) 119, 137, 309, 438, 513
TspRI CASTG 6 cut(s) 672, 897, 1002, 1335, 1416, 1434
VspI ATTAAT 1 cut(s) 327
XapI RAATTY 2 cut(s) 369, 725
XbaI TCTAGA 1 cut(s) 1553
XceI RCATGY 1 cut(s) 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 1235
XspI CTAG 3 cut(s) 41, 813, 1554
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.