MD17G1040100.v1.1

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014084
Physical Location & Seq
Forward (+)
2928948 .. 2930648
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1040100.v1.1.491

Sequence Viewer

Length: 984 bp
ATGATGGCATTCCTCATTGTCCTCCTCACATCTCTACTAGCTCTCACCACTCTCCCCGCCGCCGAATCCCAACTCTACACCAACTACTACCAAAAGTCGTGCCCGGGATTCGCCCAAATCGTCCAAGACACCGTCACCAACAAGCAGATAACGAACCCCACCACGGCCGCCGGCACCCTCCGCCTCTTCTTCCACGACTGCCTTCACAATGGCTGCGATGGCTCCATTCTCCTCTCCTCCACCCCCTTCAACAAGGCCGAGCGCGACGCCGACATCAACCTGTCCCTCCCCGGAGACGCCTTCGACGTCGTTGTCCGCGCCAAGACCGCCCTCGAGCTCGCCTGCCCCAACACCGTCTCCTGCGCTGACATCCTCGCCGTCGCCACCCGCGATCTCGTAACCATGATGGGCGGCCCCTTCTACAACGTCCCCCTCGGCCGCCGCGACGGCCGCTCCTCCAATGCCTCCGCCGTCGAAGGCACCCTCCCCCGCCCCGCCATGACCATCTCCCAGCTCATCGAGGTCTTCGGGTCCAGGGGCTTCTCCGTCCAGGAAATGGTCGCCCTCAGCGGGGCCCACACCATCGGGTTCTCCCACTGCAGCGAGTTCAGCTCGGCGATCTACAACTACAGCAAGTCGGCGCAGTCCGACCCGAATTACAACCCGAGATTCGCATCCGGGTTGCAGCAGGCCTGCGCGGATATCAAGAAGAACCCGACCCTCTCCGTCTTTAACGACGTTATGACGCCCAACAAATTCGACAATGTCTACTTCCAGAACTTGCCAAAGGGTCTGGGGGTTTTGAAATCGGACCACGCGCTGTTTAACGACGCCAAGACGCGGCCGTTTGTCGAGCTGTACGCCAACGACCAGAACCGCTTTTTTCGGGATTTTGCCAAGGCGATGGAGAAGCTCGGCGTGTTAGGGATTCAGACCGGAAAACGCGGAGAGATTAGGCACAGATGCGACGAGTCTAATTATTAG

Protein Analysis

328

Amino Acids

35.77

Weight (kDa)

6.99

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 41 - 290 7.7e-81 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014084)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28200 AT5G40150
fragaria_vesca FvH4_6g50150 FvH4_6g50150
malus_domestica MD09G1039100.v1.1 MD17G1040100.v1.1
prunus_persica Prupe.3G278900_v2.0.a1
pyrus_communis pycom111g03080
rosa_chinensis RchiOBHm_Chr2g0170391
rosa_laevigata RLG00000021953
rosa_multiflora Rmu_sc0011601.1_g000014
rosa_rugosa Rorug02G0548700
rosa_samantha Rh2AG620200 Rh2BG631100 Rh2CG601000 Rh2DG644100
rosa_wichuraiana Rw2G051400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
AatII GACGTC 1 cut(s) 309
AccB1I GGYRCC 2 cut(s) 173, 479
AccB7I CCANNNNNTGG 1 cut(s) 556
AccBSI CCGCTC 1 cut(s) 453
AccI GTMKAC 1 cut(s) 768
AccII CGCG 8 cut(s) 264, 318, 390, 444, 698, 818, 841, 945
AcoI YGGCCR 4 cut(s) 165, 436, 448, 842
AcsI RAATTY 1 cut(s) 755
AcyI GRCGYC 5 cut(s) 267, 297, 306, 746, 831
AfaI GTAC 1 cut(s) 860
AfiI CCNNNNNNNGG 5 cut(s) 163, 556, 570, 571, 840
AgsI TTSAA 2 cut(s) 250, 805
AjnI CCWGG 2 cut(s) 533, 549
AluBI AGCT 6 cut(s) 41, 337, 514, 612, 856, 913
AluI AGCT 6 cut(s) 41, 337, 514, 612, 856, 913
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 288, 361
Ama87I CYCGRG 3 cut(s) 103, 332, 664
AoxI GGCC 8 cut(s) 165, 255, 412, 436, 448, 573, 690, 842
ApaI GGGCCC 1 cut(s) 577
ApeKI GCWGC 3 cut(s) 213, 600, 685
ApoI RAATTY 1 cut(s) 755
AspLEI GCGC 6 cut(s) 264, 320, 365, 643, 698, 820
AspS9I GGNCC 5 cut(s) 413, 531, 573, 574, 811
AsuC2I CCSGG 4 cut(s) 104, 105, 291, 679
AsuHPI GGTGA 2 cut(s) 37, 127
AvaI CYCGRG 3 cut(s) 103, 332, 664
AvaII GGWCC 2 cut(s) 531, 811
BaeGI GKGCMC 2 cut(s) 104, 577
BanI GGYRCC 2 cut(s) 173, 479
BanII GRGCYC 2 cut(s) 339, 577
BbsI GAAGAC 1 cut(s) 517
Bbv12I GWGCWC 1 cut(s) 339
BbvCI CCTCAGC 1 cut(s) 566
BbvI GCAGC 3 cut(s) 200, 612, 697
BccI CCATC 5 cut(s) 212, 400, 512, 590, 898
BceAI ACGGC 5 cut(s) 180, 362, 455, 463, 829
BciT130I CCWGG 2 cut(s) 535, 551
BcnI CCSGG 4 cut(s) 104, 105, 291, 679
BcoDI GTCTC 2 cut(s) 288, 361
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 2 cut(s) 598, 628
BglI GCCNNNNNGGC 1 cut(s) 447
Bme1390I CCNGG 6 cut(s) 104, 105, 291, 535, 551, 679
Bme18I GGWCC 2 cut(s) 531, 811
BmeT110I CYCGRG 3 cut(s) 103, 332, 664
BmgT120I GGNCC 5 cut(s) 413, 531, 573, 574, 811
BmiI GGNNCC 7 cut(s) 175, 223, 415, 481, 532, 574, 575
BmrFI CCNGG 6 cut(s) 104, 105, 291, 535, 551, 679
BmsI GCATC 2 cut(s) 683, 953
BpiI GAAGAC 1 cut(s) 517
BplI GAGNNNNNCTC 2 cut(s) 596, 628
Bpu10I CCTNAGC 1 cut(s) 566
BpuMI CCSGG 4 cut(s) 104, 105, 291, 679
BsaBI GATNNNNATC 1 cut(s) 673
BsaHI GRCGYC 5 cut(s) 267, 297, 306, 746, 831
BsaJI CCNNGG 6 cut(s) 103, 162, 289, 433, 534, 897
BsaWI WCCGGW 1 cut(s) 935
BsaXI ACNNNNNCTCC 4 cut(s) 341, 371, 437, 467
Bsc4I CCNNNNNNNGG 5 cut(s) 163, 556, 570, 571, 840
Bse118I RCCGGY 1 cut(s) 170
Bse8I GATNNNNATC 1 cut(s) 673
BseBI CCWGG 2 cut(s) 535, 551
BseDI CCNNGG 6 cut(s) 103, 162, 289, 433, 534, 897
BseGI GGATG 2 cut(s) 369, 674
BseJI GATNNNNATC 1 cut(s) 673
BseLI CCNNNNNNNGG 5 cut(s) 163, 556, 570, 571, 840
BseMII CTCAG 1 cut(s) 580
BseRI GAGGAG 4 cut(s) 14, 221, 226, 445
BseSI GKGCMC 2 cut(s) 104, 577
BseX3I CGGCCG 4 cut(s) 165, 436, 448, 842
BseXI GCAGC 3 cut(s) 200, 612, 697
BseYI CCCAGC 1 cut(s) 510
Bsh1236I CGCG 8 cut(s) 264, 318, 390, 444, 698, 818, 841, 945
Bsh1285I CGRYCG 4 cut(s) 168, 439, 451, 845
BshFI GGCC 8 cut(s) 167, 257, 414, 438, 450, 575, 692, 844
BshNI GGYRCC 2 cut(s) 173, 479
BsiEI CGRYCG 4 cut(s) 168, 439, 451, 845
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 3 cut(s) 103, 332, 664
BsiSI CCGG 5 cut(s) 104, 171, 291, 678, 936
BslFI GGGAC 2 cut(s) 268, 413
BslI CCNNNNNNNGG 5 cut(s) 163, 556, 570, 571, 840
BsmAI GTCTC 2 cut(s) 288, 361
BsmBI CGTCTC 2 cut(s) 288, 361
BsmFI GGGAC 2 cut(s) 268, 413
BsmI GAATGC 1 cut(s) 8
BsnI GGCC 8 cut(s) 167, 257, 414, 438, 450, 575, 692, 844
BsoBI CYCGRG 3 cut(s) 103, 332, 664
Bsp120I GGGCCC 1 cut(s) 573
Bsp1286I GDGCHC 3 cut(s) 104, 339, 577
Bsp143I GATC 2 cut(s) 391, 618
BspANI GGCC 8 cut(s) 167, 257, 414, 438, 450, 575, 692, 844
BspCNI CTCAG 1 cut(s) 579
BspFNI CGCG 8 cut(s) 264, 318, 390, 444, 698, 818, 841, 945
BspLI GGNNCC 7 cut(s) 175, 223, 415, 481, 532, 574, 575
BspMAI CTGCAG 1 cut(s) 602
BspT107I GGYRCC 2 cut(s) 173, 479
BsrBI CCGCTC 1 cut(s) 453
BsrFI RCCGGY 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 6 cut(s) 103, 162, 289, 433, 534, 897
BssMI GATC 2 cut(s) 391, 618
BssNI GRCGYC 5 cut(s) 267, 297, 306, 746, 831
BssT1I CCWWGG 1 cut(s) 897
Bst2UI CCWGG 2 cut(s) 535, 551
Bst4CI ACNGT 2 cut(s) 133, 355
Bst6I CTCTTC 1 cut(s) 191
BstACI GRCGYC 5 cut(s) 267, 297, 306, 746, 831
BstC8I GCNNGC 5 cut(s) 172, 339, 343, 690, 694
BstDEI CTNAG 1 cut(s) 566
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 2 cut(s) 369, 674
BstFNI CGCG 8 cut(s) 264, 318, 390, 444, 698, 818, 841, 945
BstHHI GCGC 6 cut(s) 264, 320, 365, 643, 698, 820
BstKTI GATC 2 cut(s) 394, 621
BstMAI GTCTC 2 cut(s) 288, 361
BstMBI GATC 2 cut(s) 391, 618
BstMCI CGRYCG 4 cut(s) 168, 439, 451, 845
BstMWI GCNNNNNNNGC 6 cut(s) 180, 219, 326, 447, 450, 609
BstNI CCWGG 2 cut(s) 535, 551
BstSCI CCNGG 6 cut(s) 102, 103, 289, 533, 549, 677
BstSFI CTRYAG 2 cut(s) 598, 628
BstSLI GKGCMC 2 cut(s) 104, 577
BstUI CGCG 8 cut(s) 264, 318, 390, 444, 698, 818, 841, 945
BstV1I GCAGC 3 cut(s) 200, 612, 697
BstV2I GAAGAC 1 cut(s) 517
BstXI CCANNNNNNTGG 1 cut(s) 904
BstZI CGGCCG 4 cut(s) 165, 436, 448, 842
BsuRI GGCC 8 cut(s) 167, 257, 414, 438, 450, 575, 692, 844
BtgI CCRYGG 1 cut(s) 162
BtgZI GCGATG 2 cut(s) 231, 917
BtsCI GGATG 2 cut(s) 369, 674
BtsI GCAGTG 1 cut(s) 595
BtsIMutI CAGTG 1 cut(s) 595
Cac8I GCNNGC 5 cut(s) 172, 339, 343, 690, 694
CfoI GCGC 6 cut(s) 264, 320, 365, 643, 698, 820
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 5 cut(s) 413, 531, 573, 574, 811
Cfr9I CCCGGG 1 cut(s) 103
CseI GACGC 5 cut(s) 275, 305, 754, 839, 847
Csp6I GTAC 1 cut(s) 859
CviAII CATG 2 cut(s) 403, 499
CviQI GTAC 1 cut(s) 859
DdeI CTNAG 1 cut(s) 566
DpnI GATC 2 cut(s) 393, 620
DpnII GATC 2 cut(s) 391, 618
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 4 cut(s) 165, 436, 448, 842
EagI CGGCCG 4 cut(s) 165, 436, 448, 842
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
EciI GGCGGA 2 cut(s) 170, 457
Ecl136II GAGCTC 1 cut(s) 337
EclXI CGGCCG 4 cut(s) 165, 436, 448, 842
Eco130I CCWWGG 1 cut(s) 897
Eco147I AGGCCT 1 cut(s) 692
Eco24I GRGCYC 2 cut(s) 339, 577
Eco32I GATATC 1 cut(s) 703
Eco47I GGWCC 2 cut(s) 531, 811
Eco52I CGGCCG 4 cut(s) 165, 436, 448, 842
Eco53kI GAGCTC 1 cut(s) 337
Eco88I CYCGRG 3 cut(s) 103, 332, 664
EcoICRI GAGCTC 1 cut(s) 337
EcoO109I RGGNCCY 1 cut(s) 573
EcoRII CCWGG 2 cut(s) 533, 549
EcoRV GATATC 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 897
EcoT38I GRGCYC 2 cut(s) 339, 577
ErhI CCWWGG 1 cut(s) 897
Esp3I CGTCTC 2 cut(s) 288, 361
FaeI CATG 2 cut(s) 406, 502
FaiI YATR 3 cut(s) 404, 500, 743
FaqI GGGAC 2 cut(s) 268, 413
FatI CATG 2 cut(s) 402, 498
FauI CCCGC 5 cut(s) 64, 395, 497, 502, 563
FblI GTMKAC 1 cut(s) 768
FokI GGATG 2 cut(s) 356, 661
FriOI GRGCYC 2 cut(s) 339, 577
FspBI CTAG 1 cut(s) 38
GlaI GCGC 6 cut(s) 263, 319, 364, 642, 697, 819
GsaI CCCAGC 1 cut(s) 514
HaeIII GGCC 8 cut(s) 167, 257, 414, 438, 450, 575, 692, 844
HapII CCGG 5 cut(s) 104, 171, 291, 678, 936
HgaI GACGC 5 cut(s) 275, 305, 754, 839, 847
HhaI GCGC 6 cut(s) 264, 320, 365, 643, 698, 820
Hin1I GRCGYC 5 cut(s) 267, 297, 306, 746, 831
Hin1II CATG 2 cut(s) 406, 502
Hin6I GCGC 6 cut(s) 262, 318, 363, 641, 696, 818
HinP1I GCGC 6 cut(s) 262, 318, 363, 641, 696, 818
HinfI GANTC 5 cut(s) 65, 108, 669, 928, 971
HpaII CCGG 5 cut(s) 104, 171, 291, 678, 936
HphI GGTGA 2 cut(s) 37, 127
Hpy166II GTNNAC 1 cut(s) 769
Hpy188I TCNGA 3 cut(s) 649, 811, 933
Hpy188III TCNNGA 3 cut(s) 706, 775, 887
Hpy8I GTNNAC 1 cut(s) 769
Hpy99I CGWCG 9 cut(s) 269, 308, 311, 383, 449, 476, 740, 833, 971
HpyAV CCTTC 5 cut(s) 212, 256, 310, 427, 470
HpyCH4III ACNGT 2 cut(s) 133, 355
HpyCH4IV ACGT 3 cut(s) 306, 426, 738
HpyCH4V TGCA 2 cut(s) 600, 685
HpyF10VI GCNNNNNNNGC 6 cut(s) 180, 219, 326, 447, 450, 609
HpyF3I CTNAG 1 cut(s) 566
HpySE526I ACGT 3 cut(s) 306, 426, 738
Hsp92I GRCGYC 5 cut(s) 267, 297, 306, 746, 831
Hsp92II CATG 2 cut(s) 406, 502
HspAI GCGC 6 cut(s) 262, 318, 363, 641, 696, 818
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
Kzo9I GATC 2 cut(s) 391, 618
LmnI GCTCC 2 cut(s) 227, 458
Lsp1109I GCAGC 3 cut(s) 200, 612, 697
LweI GCATC 2 cut(s) 683, 953
MaeI CTAG 1 cut(s) 38
MaeII ACGT 3 cut(s) 306, 426, 738
MaeIII GTNAC 2 cut(s) 133, 397
MalI GATC 2 cut(s) 393, 620
MbiI CCGCTC 1 cut(s) 453
MboI GATC 2 cut(s) 391, 618
MboII GAAGA 4 cut(s) 178, 181, 517, 721
MhlI GDGCHC 3 cut(s) 104, 339, 577
MluCI AATT 3 cut(s) 655, 755, 976
MlyI GAGTC 1 cut(s) 980
MmeI TCCRAC 1 cut(s) 672
MroNI GCCGGC 1 cut(s) 170
MseI TTAA 2 cut(s) 732, 825
MspA1I CMGCKG 1 cut(s) 570
MspI CCGG 5 cut(s) 104, 171, 291, 678, 936
MspR9I CCNGG 6 cut(s) 104, 105, 291, 535, 551, 679
Mva1269I GAATGC 1 cut(s) 8
MvaI CCWGG 2 cut(s) 535, 551
MvnI CGCG 8 cut(s) 264, 318, 390, 444, 698, 818, 841, 945
MwoI GCNNNNNNNGC 6 cut(s) 180, 219, 326, 447, 450, 609
NaeI GCCGGC 1 cut(s) 172
NciI CCSGG 4 cut(s) 104, 105, 291, 679
NdeII GATC 2 cut(s) 391, 618
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 2 cut(s) 406, 502
NlaIV GGNNCC 7 cut(s) 175, 223, 415, 481, 532, 574, 575
NmeAIII GCCGAG 4 cut(s) 283, 414, 593, 894
NmuCI GTSAC 1 cut(s) 133
PaeR7I CTCGAG 1 cut(s) 332
PceI AGGCCT 1 cut(s) 692
PcsI WCGNNNNNNNCGW 4 cut(s) 117, 303, 387, 441
PctI GAATGC 1 cut(s) 8
PdiI GCCGGC 1 cut(s) 172
PfeI GAWTC 4 cut(s) 65, 108, 669, 928
PflFI GACNNNGTC 2 cut(s) 131, 764
PflMI CCANNNNNTGG 1 cut(s) 556
PfoI TCCNGGA 1 cut(s) 549
PleI GAGTC 1 cut(s) 979
PpsI GAGTC 1 cut(s) 979
Psp124BI GAGCTC 1 cut(s) 339
Psp6I CCWGG 2 cut(s) 533, 549
PspFI CCCAGC 1 cut(s) 510
PspGI CCWGG 2 cut(s) 533, 549
PspN4I GGNNCC 7 cut(s) 175, 223, 415, 481, 532, 574, 575
PspOMI GGGCCC 1 cut(s) 573
PspPI GGNCC 5 cut(s) 413, 531, 573, 574, 811
PspXI VCTCGAGB 1 cut(s) 332
PstI CTGCAG 1 cut(s) 602
PsyI GACNNNGTC 2 cut(s) 131, 764
RsaI GTAC 1 cut(s) 860
RsaNI GTAC 1 cut(s) 859
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 2 cut(s) 732, 825
Sau3AI GATC 2 cut(s) 391, 618
Sau96I GGNCC 5 cut(s) 413, 531, 573, 574, 811
SchI GAGTC 1 cut(s) 980
ScrFI CCNGG 6 cut(s) 104, 105, 291, 535, 551, 679
SduI GDGCHC 3 cut(s) 104, 339, 577
SfaNI GCATC 2 cut(s) 683, 953
SfcI CTRYAG 2 cut(s) 598, 628
Sfr274I CTCGAG 1 cut(s) 332
SinI GGWCC 2 cut(s) 531, 811
SlaI CTCGAG 1 cut(s) 332
SmaI CCCGGG 1 cut(s) 105
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 3 cut(s) 655, 755, 976
SseBI AGGCCT 1 cut(s) 692
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 1 cut(s) 339
StuI AGGCCT 1 cut(s) 692
StyD4I CCNGG 6 cut(s) 102, 103, 289, 533, 549, 677
StyI CCWWGG 1 cut(s) 897
TaaI ACNGT 2 cut(s) 133, 355
TaiI ACGT 3 cut(s) 309, 429, 741
TaqI TCGA 6 cut(s) 303, 333, 474, 519, 759, 852
TasI AATT 3 cut(s) 655, 755, 976
TauI GCSGC 7 cut(s) 62, 170, 414, 441, 444, 453, 844
TfiI GAWTC 4 cut(s) 65, 108, 669, 928
Tru1I TTAA 2 cut(s) 732, 825
Tru9I TTAA 2 cut(s) 732, 825
TscAI CASTG 1 cut(s) 602
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 3 cut(s) 213, 600, 685
Tsp45I GTSAC 1 cut(s) 133
TspGWI ACGGA 2 cut(s) 535, 715
TspMI CCCGGG 1 cut(s) 103
TspRI CASTG 1 cut(s) 602
Tth111I GACNNNGTC 2 cut(s) 131, 764
Van91I CCANNNNNTGG 1 cut(s) 556
VpaK11BI GGWCC 2 cut(s) 531, 811
XapI RAATTY 1 cut(s) 755
XhoI CTCGAG 1 cut(s) 332
XmaI CCCGGG 1 cut(s) 103
XmiI GTMKAC 1 cut(s) 768
XspI CTAG 1 cut(s) 38
ZraI GACGTC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.