Rw2G051400

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014084
Physical Location & Seq
Reverse (-)
79102184 .. 79103224
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G051400.1

Sequence Viewer

Length: 978 bp
ATGGCGGTGTTGGTGTTCCTCATTTTCTTCCTCACGCTCTTATCGACCTCCTTCCCCGCCGAATCCAGACTCTACACCAACTTCTACGACAAGTCGTGCCCGAGATTCACCCAGATTGTCCAGGACATTGTCACCAACAAGCAGATCTCCACCCCCACCACGGCCGCCGGAACCCTCCGCCTCTTCTTCCACGACTGCATCCACAACGGCTGCGACGCCTCCATCCTGGTCTCCTCCACCCCCTTCAACAAGGCCGAGCGCGACGCCGACATCAACCTCTCCCTCCCCGGCGACGCCTTCGACGTCGTCGTCCGCGCCAAGACCGCCCTCGAGCTCGCCTGCCCCAACACCGTCTCCTGCGCCGACATCCTCGCCGTCGCCACCCGCGACCTCGTCACCATGATGGGCGGCCCTTACTACAACGTCCCCCTCGGCCGCCGCGACGGCAAGTCCTCCCGCGCCGCCGCCGTCGAGGGCACCCTGCCCCGACCCGCCATGCCCGTCTCCGAGCTCATCCGGGTCTTCGGATCCAGAGGCTTCTCGGTTCAGGAAATGGTCGCCCTCAGCGGGGCCCACACCATCGGATTCTCCCACTGTACGGAGTTCACCGCCGCGATCTACAACTACAGCCGGGAGTCGCAGTCCGACCCGGAGTACAACCCGAGATACGCGGCCGGGTTGCGGAATGCATGCGCCGATGTCCAGAAGAACCCGACTCTGTCGGTGTTTAACGACGTCATGACGCCGAACAAGTTCGACAATGCGTATTTCCAGAACCTTCCGAAAGGATTGGGGCTGTTGAAGTCGGATCATGCTCTGTTTAACGACCCGAGAACGAGGCCGTTTGTGGAGCTTTATGCCAAGGATCAGAACAGCTTCTTCTACGCGTTTGCGAGAGCCATGGAGAAGTTGAGTGTGTATGGGATTCAGACGGGGAGGAAAGGAGAGATTAGGCACAGATGCGATGAGTTTAATTAA

Protein Analysis

325

Amino Acids

36.0

Weight (kDa)

7.57

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 41 - 289 1.1e-79 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014084)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28200 AT5G40150
fragaria_vesca FvH4_6g50150 FvH4_6g50150
malus_domestica MD09G1039100.v1.1 MD17G1040100.v1.1
prunus_persica Prupe.3G278900_v2.0.a1
pyrus_communis pycom111g03080
rosa_chinensis RchiOBHm_Chr2g0170391
rosa_laevigata RLG00000021953
rosa_multiflora Rmu_sc0011601.1_g000014
rosa_rugosa Rorug02G0548700
rosa_samantha Rh2AG620200 Rh2BG631100 Rh2CG601000 Rh2DG644100
rosa_wichuraiana Rw2G051400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
AatII GACGTC 2 cut(s) 306, 738
AccB1I GGYRCC 1 cut(s) 476
AccII CGCG 8 cut(s) 261, 315, 387, 441, 459, 614, 671, 887
AclWI GGATC 4 cut(s) 522, 535, 816, 873
AcoI YGGCCR 3 cut(s) 162, 433, 672
AcyI GRCGYC 6 cut(s) 216, 264, 294, 303, 735, 743
AfaI GTAC 2 cut(s) 598, 656
AfiI CCNNNNNNNGG 5 cut(s) 160, 567, 568, 598, 681
AflIII ACRYGT 1 cut(s) 885
AgsI TTSAA 2 cut(s) 247, 802
AhdI GACNNNNNGTC 1 cut(s) 448
AjnI CCWGG 2 cut(s) 120, 225
AluBI AGCT 4 cut(s) 334, 511, 853, 876
AluI AGCT 4 cut(s) 334, 511, 853, 876
Alw21I GWGCWC 2 cut(s) 336, 513
Alw26I GTCTC 3 cut(s) 235, 358, 508
AlwI GGATC 4 cut(s) 522, 535, 816, 873
Ama87I CYCGRG 4 cut(s) 100, 329, 661, 829
AoxI GGCC 7 cut(s) 162, 252, 409, 433, 570, 672, 839
ApaI GGGCCC 1 cut(s) 574
ApeKI GCWGC 1 cut(s) 210
Asp700I GAANNNNTTC 2 cut(s) 752, 875
AspLEI GCGC 5 cut(s) 261, 317, 362, 461, 695
AspS9I GGNCC 3 cut(s) 410, 570, 571
AsuC2I CCSGG 5 cut(s) 288, 518, 632, 650, 676
AsuHPI GGTGA 4 cut(s) 100, 124, 388, 598
AvaI CYCGRG 4 cut(s) 100, 329, 661, 829
BaeGI GKGCMC 3 cut(s) 101, 479, 574
BamHI GGATCC 1 cut(s) 527
BanI GGYRCC 1 cut(s) 476
BanII GRGCYC 3 cut(s) 336, 513, 574
BbsI GAAGAC 1 cut(s) 514
Bbv12I GWGCWC 2 cut(s) 336, 513
BbvCI CCTCAGC 1 cut(s) 563
BbvI GCAGC 1 cut(s) 197
BccI CCATC 3 cut(s) 230, 397, 587
BceAI ACGGC 6 cut(s) 177, 223, 359, 452, 460, 826
BciT130I CCWGG 2 cut(s) 122, 227
BcnI CCSGG 5 cut(s) 288, 518, 632, 650, 676
BcoDI GTCTC 3 cut(s) 235, 358, 508
BfmI CTRYAG 1 cut(s) 625
BglI GCCNNNNNGGC 1 cut(s) 444
BglII AGATCT 1 cut(s) 144
BisI GCNGC 9 cut(s) 165, 211, 409, 436, 439, 462, 465, 612, 672
BlsI GCNGC 9 cut(s) 166, 212, 410, 437, 440, 463, 466, 613, 673
Bme1390I CCNGG 7 cut(s) 122, 227, 288, 518, 632, 650, 676
BmeRI GACNNNNNGTC 1 cut(s) 448
BmeT110I CYCGRG 4 cut(s) 100, 329, 661, 829
BmgT120I GGNCC 3 cut(s) 410, 570, 571
BmiI GGNNCC 5 cut(s) 172, 478, 529, 571, 572
BmrFI CCNGG 7 cut(s) 122, 227, 288, 518, 632, 650, 676
BmsI GCATC 2 cut(s) 207, 950
BpiI GAAGAC 1 cut(s) 514
Bpu10I CCTNAGC 1 cut(s) 563
BpuMI CCSGG 5 cut(s) 288, 518, 632, 650, 676
BsaHI GRCGYC 6 cut(s) 216, 264, 294, 303, 735, 743
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 5 cut(s) 159, 286, 430, 861, 900
BsaXI ACNNNNNCTCC 4 cut(s) 338, 368, 626, 656
Bsc4I CCNNNNNNNGG 5 cut(s) 160, 567, 568, 598, 681
BseBI CCWGG 2 cut(s) 122, 227
BseDI CCNNGG 5 cut(s) 159, 286, 430, 861, 900
BseGI GGATG 4 cut(s) 198, 222, 366, 513
BseLI CCNNNNNNNGG 5 cut(s) 160, 567, 568, 598, 681
BseMII CTCAG 1 cut(s) 577
BseRI GAGGAG 1 cut(s) 223
BseSI GKGCMC 3 cut(s) 101, 479, 574
BseX3I CGGCCG 3 cut(s) 162, 433, 672
BseXI GCAGC 1 cut(s) 197
Bsh1236I CGCG 8 cut(s) 261, 315, 387, 441, 459, 614, 671, 887
Bsh1285I CGRYCG 3 cut(s) 165, 436, 675
BshFI GGCC 7 cut(s) 164, 254, 411, 435, 572, 674, 841
BshNI GGYRCC 1 cut(s) 476
BsiEI CGRYCG 3 cut(s) 165, 436, 675
BsiHKAI GWGCWC 2 cut(s) 336, 513
BsiHKCI CYCGRG 4 cut(s) 100, 329, 661, 829
BsiSI CCGG 6 cut(s) 168, 288, 517, 631, 650, 675
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 5 cut(s) 160, 567, 568, 598, 681
BsmAI GTCTC 3 cut(s) 235, 358, 508
BsmBI CGTCTC 2 cut(s) 358, 508
BsmFI GGGAC 1 cut(s) 410
BsmI GAATGC 1 cut(s) 691
BsnI GGCC 7 cut(s) 164, 254, 411, 435, 572, 674, 841
Bso31I GGTCTC 1 cut(s) 235
BsoBI CYCGRG 4 cut(s) 100, 329, 661, 829
Bsp120I GGGCCC 1 cut(s) 570
Bsp1286I GDGCHC 5 cut(s) 101, 336, 479, 513, 574
Bsp143I GATC 5 cut(s) 144, 527, 615, 808, 865
Bsp19I CCATGG 1 cut(s) 900
BspANI GGCC 7 cut(s) 164, 254, 411, 435, 572, 674, 841
BspCNI CTCAG 1 cut(s) 576
BspFNI CGCG 8 cut(s) 261, 315, 387, 441, 459, 614, 671, 887
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 5 cut(s) 172, 478, 529, 571, 572
BspPI GGATC 4 cut(s) 522, 535, 816, 873
BspT107I GGYRCC 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 235
BssECI CCNNGG 5 cut(s) 159, 286, 430, 861, 900
BssMI GATC 5 cut(s) 144, 527, 615, 808, 865
BssNI GRCGYC 6 cut(s) 216, 264, 294, 303, 735, 743
BssT1I CCWWGG 2 cut(s) 861, 900
Bst2UI CCWGG 2 cut(s) 122, 227
Bst4CI ACNGT 2 cut(s) 352, 596
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 6 cut(s) 216, 264, 294, 303, 735, 743
BstC8I GCNNGC 3 cut(s) 336, 340, 691
BstDEI CTNAG 1 cut(s) 563
BstDSI CCRYGG 2 cut(s) 159, 900
BstF5I GGATG 4 cut(s) 198, 222, 366, 513
BstFNI CGCG 8 cut(s) 261, 315, 387, 441, 459, 614, 671, 887
BstHHI GCGC 5 cut(s) 261, 317, 362, 461, 695
BstKTI GATC 5 cut(s) 147, 530, 618, 811, 868
BstMAI GTCTC 3 cut(s) 235, 358, 508
BstMBI GATC 5 cut(s) 144, 527, 615, 808, 865
BstMCI CGRYCG 3 cut(s) 165, 436, 675
BstMWI GCNNNNNNNGC 2 cut(s) 323, 444
BstNI CCWGG 2 cut(s) 122, 227
BstNSI RCATGY 1 cut(s) 693
BstSCI CCNGG 7 cut(s) 120, 225, 286, 516, 630, 648, 674
BstSFI CTRYAG 1 cut(s) 625
BstSLI GKGCMC 3 cut(s) 101, 479, 574
BstUI CGCG 8 cut(s) 261, 315, 387, 441, 459, 614, 671, 887
BstV1I GCAGC 1 cut(s) 197
BstV2I GAAGAC 1 cut(s) 514
BstX2I RGATCY 2 cut(s) 144, 527
BstYI RGATCY 2 cut(s) 144, 527
BstZI CGGCCG 3 cut(s) 162, 433, 672
BsuRI GGCC 7 cut(s) 164, 254, 411, 435, 572, 674, 841
BtgI CCRYGG 2 cut(s) 159, 900
BtsCI GGATG 4 cut(s) 198, 222, 366, 513
BtsIMutI CAGTG 1 cut(s) 592
Cac8I GCNNGC 3 cut(s) 336, 340, 691
CciI TCATGA 1 cut(s) 738
CfoI GCGC 5 cut(s) 261, 317, 362, 461, 695
Cfr13I GGNCC 3 cut(s) 410, 570, 571
CseI GACGC 4 cut(s) 224, 272, 302, 751
Csp6I GTAC 2 cut(s) 597, 655
CviAII CATG 6 cut(s) 400, 496, 690, 739, 812, 901
CviQI GTAC 2 cut(s) 597, 655
DdeI CTNAG 1 cut(s) 563
DpnI GATC 5 cut(s) 146, 529, 617, 810, 867
DpnII GATC 5 cut(s) 144, 527, 615, 808, 865
DrdI GACNNNNNNGTC 1 cut(s) 308
DriI GACNNNNNGTC 1 cut(s) 448
DseDI GACNNNNNNGTC 1 cut(s) 308
EaeI YGGCCR 3 cut(s) 162, 433, 672
EagI CGGCCG 3 cut(s) 162, 433, 672
Eam1104I CTCTTC 1 cut(s) 188
Eam1105I GACNNNNNGTC 1 cut(s) 448
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 2 cut(s) 334, 511
EclXI CGGCCG 3 cut(s) 162, 433, 672
Eco130I CCWWGG 2 cut(s) 861, 900
Eco24I GRGCYC 3 cut(s) 336, 513, 574
Eco31I GGTCTC 1 cut(s) 235
Eco52I CGGCCG 3 cut(s) 162, 433, 672
Eco53kI GAGCTC 2 cut(s) 334, 511
Eco88I CYCGRG 4 cut(s) 100, 329, 661, 829
EcoICRI GAGCTC 2 cut(s) 334, 511
EcoO109I RGGNCCY 1 cut(s) 570
EcoRII CCWGG 2 cut(s) 120, 225
EcoT14I CCWWGG 2 cut(s) 861, 900
EcoT22I ATGCAT 1 cut(s) 691
EcoT38I GRGCYC 3 cut(s) 336, 513, 574
ErhI CCWWGG 2 cut(s) 861, 900
Esp3I CGTCTC 2 cut(s) 358, 508
FaeI CATG 6 cut(s) 403, 499, 693, 742, 815, 904
FaiI YATR 8 cut(s) 401, 497, 691, 740, 813, 858, 902, 921
FaqI GGGAC 1 cut(s) 410
FatI CATG 6 cut(s) 399, 495, 689, 738, 811, 900
FauI CCCGC 5 cut(s) 64, 392, 464, 499, 560
Fnu4HI GCNGC 9 cut(s) 165, 211, 409, 436, 439, 462, 465, 612, 672
FokI GGATG 4 cut(s) 185, 209, 353, 500
FriOI GRGCYC 3 cut(s) 336, 513, 574
Fsp4HI GCNGC 9 cut(s) 165, 211, 409, 436, 439, 462, 465, 612, 672
GlaI GCGC 5 cut(s) 260, 316, 361, 460, 694
GluI GCNGC 9 cut(s) 165, 211, 409, 436, 439, 462, 465, 612, 672
HaeIII GGCC 7 cut(s) 164, 254, 411, 435, 572, 674, 841
HapII CCGG 6 cut(s) 168, 288, 517, 631, 650, 675
HgaI GACGC 4 cut(s) 224, 272, 302, 751
HhaI GCGC 5 cut(s) 261, 317, 362, 461, 695
Hin1I GRCGYC 6 cut(s) 216, 264, 294, 303, 735, 743
Hin1II CATG 6 cut(s) 403, 499, 693, 742, 815, 904
Hin6I GCGC 5 cut(s) 259, 315, 360, 459, 693
HinP1I GCGC 5 cut(s) 259, 315, 360, 459, 693
HinfI GANTC 7 cut(s) 62, 69, 105, 585, 635, 715, 925
HpaII CCGG 6 cut(s) 168, 288, 517, 631, 650, 675
HphI GGTGA 4 cut(s) 100, 124, 388, 598
Hpy166II GTNNAC 1 cut(s) 606
Hpy188I TCNGA 8 cut(s) 508, 527, 584, 646, 783, 808, 870, 930
Hpy188III TCNNGA 6 cut(s) 66, 531, 548, 703, 739, 772
Hpy8I GTNNAC 1 cut(s) 606
HpyAV CCTTC 4 cut(s) 61, 253, 307, 788
HpyCH4III ACNGT 2 cut(s) 352, 596
HpyCH4IV ACGT 3 cut(s) 303, 423, 735
HpyCH4V TGCA 2 cut(s) 198, 689
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 444
HpyF3I CTNAG 1 cut(s) 563
HpySE526I ACGT 3 cut(s) 303, 423, 735
Hsp92I GRCGYC 6 cut(s) 216, 264, 294, 303, 735, 743
Hsp92II CATG 6 cut(s) 403, 499, 693, 742, 815, 904
HspAI GCGC 5 cut(s) 259, 315, 360, 459, 693
Kzo9I GATC 5 cut(s) 144, 527, 615, 808, 865
LmnI GCTCC 1 cut(s) 850
Lsp1109I GCAGC 1 cut(s) 197
LweI GCATC 2 cut(s) 207, 950
MaeII ACGT 3 cut(s) 303, 423, 735
MaeIII GTNAC 2 cut(s) 130, 394
MalI GATC 5 cut(s) 146, 529, 617, 810, 867
MboI GATC 5 cut(s) 144, 527, 615, 808, 865
MboII GAAGA 6 cut(s) 19, 175, 178, 514, 718, 871
MflI RGATCY 2 cut(s) 144, 527
MhlI GDGCHC 5 cut(s) 101, 336, 479, 513, 574
MluCI AATT 1 cut(s) 973
MluI ACGCGT 1 cut(s) 885
MlyI GAGTC 3 cut(s) 63, 644, 709
MmeI TCCRAC 2 cut(s) 669, 786
Mph1103I ATGCAT 1 cut(s) 691
MroXI GAANNNNTTC 2 cut(s) 752, 875
MseI TTAA 4 cut(s) 729, 822, 972, 976
MslI CAYNNNNRTG 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 567
MspI CCGG 6 cut(s) 168, 288, 517, 631, 650, 675
MspR9I CCNGG 7 cut(s) 122, 227, 288, 518, 632, 650, 676
Mva1269I GAATGC 1 cut(s) 691
MvaI CCWGG 2 cut(s) 122, 227
MvnI CGCG 8 cut(s) 261, 315, 387, 441, 459, 614, 671, 887
MwoI GCNNNNNNNGC 2 cut(s) 323, 444
NciI CCSGG 5 cut(s) 288, 518, 632, 650, 676
NcoI CCATGG 1 cut(s) 900
NdeII GATC 5 cut(s) 144, 527, 615, 808, 865
NlaIII CATG 6 cut(s) 403, 499, 693, 742, 815, 904
NlaIV GGNNCC 5 cut(s) 172, 478, 529, 571, 572
NmeAIII GCCGAG 2 cut(s) 280, 411
NmuCI GTSAC 2 cut(s) 130, 394
NsiI ATGCAT 1 cut(s) 691
NspI RCATGY 1 cut(s) 693
PacI TTAATTAA 1 cut(s) 976
PaeI GCATGC 1 cut(s) 693
PaeR7I CTCGAG 1 cut(s) 329
PagI TCATGA 1 cut(s) 738
PcsI WCGNNNNNNNCGW 5 cut(s) 41, 300, 306, 384, 438
PctI GAATGC 1 cut(s) 691
PdmI GAANNNNTTC 2 cut(s) 752, 875
PfeI GAWTC 4 cut(s) 62, 105, 585, 925
PflFI GACNNNGTC 4 cut(s) 128, 305, 392, 718
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 9 cut(s) 166, 212, 410, 437, 440, 463, 466, 613, 673
PleI GAGTC 3 cut(s) 63, 643, 709
PpsI GAGTC 3 cut(s) 63, 643, 709
Psp124BI GAGCTC 2 cut(s) 336, 513
Psp6I CCWGG 2 cut(s) 120, 225
PspGI CCWGG 2 cut(s) 120, 225
PspN4I GGNNCC 5 cut(s) 172, 478, 529, 571, 572
PspOMI GGGCCC 1 cut(s) 570
PspPI GGNCC 3 cut(s) 410, 570, 571
PspXI VCTCGAGB 1 cut(s) 329
PsuI RGATCY 2 cut(s) 144, 527
PsyI GACNNNGTC 4 cut(s) 128, 305, 392, 718
RsaI GTAC 2 cut(s) 598, 656
RsaNI GTAC 2 cut(s) 597, 655
RseI CAYNNNNRTG 1 cut(s) 401
SacI GAGCTC 2 cut(s) 336, 513
SaqAI TTAA 4 cut(s) 729, 822, 972, 976
SatI GCNGC 9 cut(s) 165, 211, 409, 436, 439, 462, 465, 612, 672
Sau3AI GATC 5 cut(s) 144, 527, 615, 808, 865
Sau96I GGNCC 3 cut(s) 410, 570, 571
SchI GAGTC 3 cut(s) 63, 644, 709
ScrFI CCNGG 7 cut(s) 122, 227, 288, 518, 632, 650, 676
SduI GDGCHC 5 cut(s) 101, 336, 479, 513, 574
SfaNI GCATC 2 cut(s) 207, 950
SfcI CTRYAG 1 cut(s) 625
Sfr274I CTCGAG 1 cut(s) 329
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SphI GCATGC 1 cut(s) 693
Sse9I AATT 1 cut(s) 973
SstI GAGCTC 2 cut(s) 336, 513
StyD4I CCNGG 7 cut(s) 120, 225, 286, 516, 630, 648, 674
StyI CCWWGG 2 cut(s) 861, 900
TaaI ACNGT 2 cut(s) 352, 596
TaiI ACGT 3 cut(s) 306, 426, 738
TaqI TCGA 5 cut(s) 44, 300, 330, 471, 756
TasI AATT 1 cut(s) 973
TatI WGTACW 1 cut(s) 654
TauI GCSGC 8 cut(s) 167, 411, 438, 441, 464, 467, 614, 674
TfiI GAWTC 4 cut(s) 62, 105, 585, 925
Tru1I TTAA 4 cut(s) 729, 822, 972, 976
Tru9I TTAA 4 cut(s) 729, 822, 972, 976
TscAI CASTG 1 cut(s) 599
TseFI GTSAC 2 cut(s) 130, 394
TseI GCWGC 1 cut(s) 210
Tsp45I GTSAC 2 cut(s) 130, 394
TspGWI ACGGA 1 cut(s) 614
TspRI CASTG 1 cut(s) 599
Tth111I GACNNNGTC 4 cut(s) 128, 305, 392, 718
XceI RCATGY 1 cut(s) 693
XhoI CTCGAG 1 cut(s) 329
XmnI GAANNNNTTC 2 cut(s) 752, 875
ZraI GACGTC 2 cut(s) 304, 736
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.