MD17G1070800.v1.1

Stress-induced protein KIN2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009815
Physical Location & Seq
Forward (+)
5726058 .. 5726835
778 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1070800.v1.1.491

Sequence Viewer

Length: 207 bp
ATGAACCAATCCCAGAACATGAGCCACCAAGCTGGCCAGGCCAAGGGCCAAGCTCAAGAAAAGGCCAGTGGCATGATGGACCAAATGGGCAATGCTGCCCAATCTGCCAAGGAATCAGCCCAGGGGGCTGGTCAGCAAATGATGGAGAAGGCTCAGGGAGCAGCTGACTCCGTCAAGGATGCAGTTGGCGCCAACAAAAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

69

Amino Acids

6.98

Weight (kDa)

8.12

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AcoI YGGCCR 1 cut(s) 34
AcyI GRCGYC 1 cut(s) 189
AfiI CCNNNNNNNGG 1 cut(s) 43
AjnI CCWGG 2 cut(s) 36, 120
AluBI AGCT 4 cut(s) 32, 53, 164, 204
AluI AGCT 4 cut(s) 32, 53, 164, 204
AoxI GGCC 4 cut(s) 34, 39, 46, 63
ApeKI GCWGC 2 cut(s) 95, 161
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 2 cut(s) 46, 79
AvaII GGWCC 1 cut(s) 79
BalI TGGCCA 1 cut(s) 36
BanI GGYRCC 1 cut(s) 188
BbvI GCAGC 2 cut(s) 82, 173
BccI CCATC 2 cut(s) 70, 136
BciT130I CCWGG 2 cut(s) 38, 122
BfoI RGCGCY 1 cut(s) 192
BglI GCCNNNNNGGC 1 cut(s) 125
BisI GCNGC 2 cut(s) 96, 162
BlsI GCNGC 2 cut(s) 97, 163
Bme1390I CCNGG 2 cut(s) 38, 122
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 2 cut(s) 46, 79
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 2 cut(s) 38, 122
BmsI GCATC 1 cut(s) 169
Bpu10I CCTNAGC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 4 cut(s) 42, 108, 120, 121
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse1I ACTGG 1 cut(s) 66
Bse3DI GCAATG 1 cut(s) 97
BseBI CCWGG 2 cut(s) 38, 122
BseDI CCNNGG 4 cut(s) 42, 108, 120, 121
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMI GCAATG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 66
BseXI GCAGC 2 cut(s) 82, 173
BshFI GGCC 4 cut(s) 36, 41, 48, 65
BshNI GGYRCC 1 cut(s) 188
BslI CCNNNNNNNGG 1 cut(s) 43
BsnI GGCC 4 cut(s) 36, 41, 48, 65
BspANI GGCC 4 cut(s) 36, 41, 48, 65
BspCNI CTCAG 1 cut(s) 166
BspLI GGNNCC 1 cut(s) 190
BspT107I GGYRCC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 97
BsrI ACTGG 1 cut(s) 66
BssECI CCNNGG 4 cut(s) 42, 108, 120, 121
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 2 cut(s) 42, 108
Bst2UI CCWGG 2 cut(s) 38, 122
BstACI GRCGYC 1 cut(s) 189
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 184
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 1 cut(s) 191
BstMWI GCNNNNNNNGC 5 cut(s) 38, 104, 125, 158, 188
BstNI CCWGG 2 cut(s) 38, 122
BstSCI CCNGG 2 cut(s) 36, 120
BstV1I GCAGC 2 cut(s) 82, 173
BstXI CCANNNNNNTGG 2 cut(s) 32, 128
BsuRI GGCC 4 cut(s) 36, 41, 48, 65
BtsCI GGATG 1 cut(s) 184
BtsIMutI CAGTG 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 1 cut(s) 191
Cfr13I GGNCC 2 cut(s) 46, 79
CviAII CATG 2 cut(s) 19, 73
DdeI CTNAG 1 cut(s) 153
DinI GGCGCC 1 cut(s) 190
EaeI YGGCCR 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 42, 108
Eco47I GGWCC 1 cut(s) 79
EcoRII CCWGG 2 cut(s) 36, 120
EcoT14I CCWWGG 2 cut(s) 42, 108
EgeI GGCGCC 1 cut(s) 190
EheI GGCGCC 1 cut(s) 190
ErhI CCWWGG 2 cut(s) 42, 108
FaeI CATG 2 cut(s) 22, 76
FaiI YATR 2 cut(s) 20, 74
FatI CATG 2 cut(s) 18, 72
Fnu4HI GCNGC 2 cut(s) 96, 162
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 2 cut(s) 96, 162
GlaI GCGC 1 cut(s) 190
GluI GCNGC 2 cut(s) 96, 162
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 4 cut(s) 36, 41, 48, 65
HhaI GCGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 2 cut(s) 22, 76
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HinfI GANTC 2 cut(s) 113, 167
Hpy188III TCNNGA 1 cut(s) 56
HpyAV CCTTC 1 cut(s) 142
HpyCH4V TGCA 1 cut(s) 182
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 104, 125, 158, 188
HpyF3I CTNAG 1 cut(s) 153
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 2 cut(s) 22, 76
HspAI GCGC 1 cut(s) 189
KasI GGCGCC 1 cut(s) 188
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 9 cut(s) 18, 23, 26, 50, 79, 107, 114, 134, 140
Lsp1109I GCAGC 2 cut(s) 82, 173
LweI GCATC 1 cut(s) 169
MlsI TGGCCA 1 cut(s) 36
MluNI TGGCCA 1 cut(s) 36
Mly113I GGCGCC 1 cut(s) 189
MlyI GAGTC 1 cut(s) 161
Mox20I TGGCCA 1 cut(s) 36
MscI TGGCCA 1 cut(s) 36
Msp20I TGGCCA 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 164
MspR9I CCNGG 2 cut(s) 38, 122
MvaI CCWGG 2 cut(s) 38, 122
MwoI GCNNNNNNNGC 5 cut(s) 38, 104, 125, 158, 188
NarI GGCGCC 1 cut(s) 189
NlaIII CATG 2 cut(s) 22, 76
NlaIV GGNNCC 1 cut(s) 190
PasI CCCWGGG 1 cut(s) 121
PfeI GAWTC 1 cut(s) 113
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 2 cut(s) 97, 163
PleI GAGTC 1 cut(s) 161
PluTI GGCGCC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 161
Psp6I CCWGG 2 cut(s) 36, 120
PspGI CCWGG 2 cut(s) 36, 120
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 2 cut(s) 46, 79
PsyI GACNNNGTC 1 cut(s) 170
PvuII CAGCTG 1 cut(s) 164
SatI GCNGC 2 cut(s) 96, 162
Sau96I GGNCC 2 cut(s) 46, 79
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 2 cut(s) 38, 122
SetI ASST 4 cut(s) 34, 55, 166, 206
SfaNI GCATC 1 cut(s) 169
SfoI GGCGCC 1 cut(s) 190
SinI GGWCC 1 cut(s) 79
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
SspDI GGCGCC 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 36, 120
StyI CCWWGG 2 cut(s) 42, 108
TfiI GAWTC 1 cut(s) 113
TscAI CASTG 1 cut(s) 73
TseI GCWGC 2 cut(s) 95, 161
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 1 cut(s) 73
Tth111I GACNNNGTC 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 79
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.