MD17G1091900.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0018493
Physical Location & Seq
Reverse (-)
7702460 .. 7706549
4090 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1091900.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGACTCAGTGCACCCAAGACCTAGCAGAAAATAGCTGCTTAATTTGCCTGCAGGAGATTGCCAGTTATGTGTCGGAGTGCTGTGATGGATTTGTTGGGATTACACTAATTTCTAAGAGCTGTTATCTCCGGTATGAGATGTATTCGTTTTTCCAGTCACCATTACAACATCCACCACCTGCAGCGGCATCTTCTCTATTTCCTTCATTGCTTGAGTCTAACTCAAGTACTACTAGCGGCACTAATAAGACTAGTCCTGGTAAAATTTTAGGTTCTTTTGATGCATTCAAAAGCTTCCTGCATGCTCATGTAAAATCTACAAGATGCTGCGCTTGTTGTTTGCTGTGGGAAAGGACCAGGAGAAATGGAGTTGGACTACATACACTTAAAGTTGCAACCGGCAATTTCTCAGTAGCATATAAACTCGGAGAAGGTGGATTTGGTCCAGTTTACAAGGGAAAACTGCCTGATGGACAGGACATAACGGTGAAAAGGCTGTCACATAGTTCAGGCCAAGGTCTAGCAGAACTGAAAACCGAAGTAATGCTCGTTGCTAAGTTACTCCACTGGAATCTGGTGAGACTGCTGGGCTTCTGCTCAGAAGATGAAGAGATGTTACTCGTCTACGAATACCTACCCAACGGGAGCTTGGACAAGATATTATTTGATCATGGGAGAACATTTAGTTTGGAATGGGAAAGAAGATTCAAAATCATCGTTGGAATTGCACGAGGGCTACTTTATCTGCACGAAGATTTTCAACTTAGAATTATTTATCGAGATTTGAAACCCAGCAACATCCTCTTAGACAAAGACATGAACCCTAAAATATCGGATTTTGGTTTGGCAAAACTGTTCCACGGAAGCCAAACTCATGGCAATACATATCGCATATCTGGTACTTTGTATGCATGGCAATATTGGGCCAAGGGAACATCATTGGAGTTATTGGATCCAAGTCTGGGAGACCAGTGGCCTAGATATGAGGTCTTGAAGTGTATCCACATTGGGTTGCTATGCGTTCAAGAAGCTTCGGCTGAAAGACCTACCATGTCGGAGGTTGTTATGATGCTCAATAGTTACTCTACAACCTCCGCTGTGCCGTCGCAGCCAGCGTTTTATATCCGGCAGGAAAGCTCGACAAATTCACAGAAGTCTGGTGGATTTGAACTCGATAAGTTAGAAGATGCAGCTCAATCGGTAAATGACCTTACAGTCACTCAACACCATCCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

413

Amino Acids

46.06

Weight (kDa)

6.67

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 138 - 295 9.5e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 139 - 290 2.7e-29 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018493)

Species Orthologous Gene IDs
malus_domestica MD09G1104200.v1.1 MD17G1091900.v1.1
prunus_persica Prupe.3G221500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0160201
rosa_laevigata RLG00000021204
rosa_rugosa Rorug02G0482400
rosa_samantha Rh2BG561000 Rh2CG531600 Rh2DG569700
rosa_wichuraiana Rw2G045300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 187
AasI GACNNNNNNGTC 1 cut(s) 1214
Acc36I ACCTGC 1 cut(s) 187
AccI GTMKAC 1 cut(s) 624
AciI CCGC 3 cut(s) 185, 237, 1095
AclWI GGATC 2 cut(s) 947, 960
AcsI RAATTY 2 cut(s) 264, 1144
AfaI GTAC 2 cut(s) 229, 901
AfiI CCNNNNNNNGG 1 cut(s) 962
AgsI TTSAA 7 cut(s) 289, 709, 761, 787, 994, 1025, 1169
AhlI ACTAGT 1 cut(s) 251
AjnI CCWGG 2 cut(s) 256, 356
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AluBI AGCT 7 cut(s) 36, 120, 294, 648, 1031, 1137, 1193
AluI AGCT 7 cut(s) 36, 120, 294, 648, 1031, 1137, 1193
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 2 cut(s) 574, 960
Alw44I GTGCAC 1 cut(s) 10
AlwI GGATC 2 cut(s) 947, 960
AoxI GGCC 3 cut(s) 511, 924, 974
ApaLI GTGCAC 1 cut(s) 10
ApeKI GCWGC 5 cut(s) 36, 182, 327, 1108, 1190
ApoI RAATTY 2 cut(s) 264, 1144
Asp700I GAANNNNTTC 1 cut(s) 756
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 3 cut(s) 354, 443, 924
AsuHPI GGTGA 3 cut(s) 150, 499, 589
AvaII GGWCC 2 cut(s) 354, 443
BaeGI GKGCMC 1 cut(s) 14
BamHI GGATCC 1 cut(s) 952
BarI GAAGNNNNNNTAC 2 cut(s) 600, 632
BauI CACGAG 1 cut(s) 729
Bbv12I GWGCWC 1 cut(s) 14
BbvI GCAGC 5 cut(s) 23, 194, 314, 1120, 1202
BccI CCATC 3 cut(s) 80, 464, 1236
BceAI ACGGC 1 cut(s) 1087
BcgI CGANNNNNNTGC 2 cut(s) 1179, 1213
BciT130I CCWGG 2 cut(s) 258, 358
BciVI GTATCC 1 cut(s) 1010
BclI TGATCA 1 cut(s) 667
BcoDI GTCTC 2 cut(s) 574, 960
BcuI ACTAGT 1 cut(s) 251
BfaI CTAG 6 cut(s) 23, 234, 252, 521, 978, 1237
BfmI CTRYAG 2 cut(s) 50, 180
BfuAI ACCTGC 1 cut(s) 187
BfuI GTATCC 1 cut(s) 1010
BisI GCNGC 7 cut(s) 37, 183, 186, 238, 328, 1109, 1191
BlsI GCNGC 7 cut(s) 38, 184, 187, 239, 329, 1110, 1192
BmcAI AGTACT 1 cut(s) 229
Bme1390I CCNGG 2 cut(s) 258, 358
Bme18I GGWCC 2 cut(s) 354, 443
BmgT120I GGNCC 3 cut(s) 354, 443, 924
BmiI GGNNCC 1 cut(s) 954
BmrFI CCNGG 2 cut(s) 258, 358
BmsI GCATC 5 cut(s) 197, 271, 314, 1059, 1177
BplI GAGNNNNNCTC 2 cut(s) 206, 238
BpuEI CTTGAG 2 cut(s) 208, 233
BsaI GGTCTC 1 cut(s) 960
BsaJI CCNNGG 3 cut(s) 514, 859, 927
BsaWI WCCGGW 1 cut(s) 129
Bsc4I CCNNNNNNNGG 1 cut(s) 962
Bse118I RCCGGY 1 cut(s) 398
Bse1I ACTGG 5 cut(s) 63, 154, 446, 572, 970
Bse3DI GCAATG 1 cut(s) 206
BseBI CCWGG 2 cut(s) 258, 358
BseDI CCNNGG 3 cut(s) 514, 859, 927
BseGI GGATG 3 cut(s) 169, 798, 1228
BseLI CCNNNNNNNGG 1 cut(s) 962
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 3 cut(s) 20, 423, 612
BseNI ACTGG 5 cut(s) 63, 154, 446, 572, 970
BseSI GKGCMC 1 cut(s) 14
BseXI GCAGC 5 cut(s) 23, 194, 314, 1120, 1202
BseYI CCCAGC 2 cut(s) 586, 791
BsgI GTGCAG 1 cut(s) 731
BshFI GGCC 3 cut(s) 513, 926, 976
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 3 cut(s) 130, 399, 1126
BslI CCNNNNNNNGG 1 cut(s) 962
BsmAI GTCTC 2 cut(s) 574, 960
BsmI GAATGC 1 cut(s) 284
BsnI GGCC 3 cut(s) 513, 926, 976
Bso31I GGTCTC 1 cut(s) 960
Bsp1286I GDGCHC 1 cut(s) 14
Bsp143I GATC 2 cut(s) 667, 952
BspACI CCGC 3 cut(s) 185, 237, 1095
BspANI GGCC 3 cut(s) 513, 926, 976
BspCNI CTCAG 3 cut(s) 19, 422, 611
BspLI GGNNCC 1 cut(s) 954
BspMAI CTGCAG 2 cut(s) 54, 184
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 2 cut(s) 947, 960
BspTNI GGTCTC 1 cut(s) 960
BsrDI GCAATG 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 398
BsrI ACTGG 5 cut(s) 63, 154, 446, 572, 970
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 3 cut(s) 514, 859, 927
BssMI GATC 2 cut(s) 667, 952
BssSI CACGAG 1 cut(s) 729
BssT1I CCWWGG 2 cut(s) 514, 927
Bst2BI CACGAG 1 cut(s) 729
Bst2UI CCWGG 2 cut(s) 258, 358
Bst4CI ACNGT 3 cut(s) 487, 855, 1216
Bst6I CTCTTC 1 cut(s) 603
BstC8I GCNNGC 3 cut(s) 50, 303, 1113
BstDEI CTNAG 7 cut(s) 6, 114, 409, 555, 598, 764, 805
BstDSI CCRYGG 1 cut(s) 859
BstF5I GGATG 3 cut(s) 169, 798, 1228
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 2 cut(s) 670, 955
BstMAI GTCTC 2 cut(s) 574, 960
BstMBI GATC 2 cut(s) 667, 952
BstMWI GCNNNNNNNGC 2 cut(s) 45, 1108
BstNI CCWGG 2 cut(s) 258, 358
BstNSI RCATGY 1 cut(s) 305
BstSCI CCNGG 2 cut(s) 256, 356
BstSFI CTRYAG 2 cut(s) 50, 180
BstSLI GKGCMC 1 cut(s) 14
BstV1I GCAGC 5 cut(s) 23, 194, 314, 1120, 1202
BstX2I RGATCY 1 cut(s) 952
BstXI CCANNNNNNTGG 1 cut(s) 875
BstYI RGATCY 1 cut(s) 952
BsuI GTATCC 1 cut(s) 1010
BsuRI GGCC 3 cut(s) 513, 926, 976
BtgI CCRYGG 1 cut(s) 859
BtsCI GGATG 3 cut(s) 169, 798, 1228
BtsIMutI CAGTG 3 cut(s) 14, 565, 977
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 3 cut(s) 50, 303, 1113
CfoI GCGC 1 cut(s) 332
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 3 cut(s) 354, 443, 924
Csp6I GTAC 2 cut(s) 228, 900
CviAII CATG 7 cut(s) 302, 308, 671, 817, 875, 912, 1051
CviQI GTAC 2 cut(s) 228, 900
DdeI CTNAG 7 cut(s) 6, 114, 409, 555, 598, 764, 805
DpnI GATC 2 cut(s) 669, 954
DpnII GATC 2 cut(s) 667, 952
DrdI GACNNNNNNGTC 1 cut(s) 1214
DseDI GACNNNNNNGTC 1 cut(s) 1214
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Eco130I CCWWGG 2 cut(s) 514, 927
Eco31I GGTCTC 1 cut(s) 960
Eco47I GGWCC 2 cut(s) 354, 443
EcoRII CCWGG 2 cut(s) 256, 356
EcoT14I CCWWGG 2 cut(s) 514, 927
EcoT22I ATGCAT 2 cut(s) 286, 913
ErhI CCWWGG 2 cut(s) 514, 927
FaeI CATG 7 cut(s) 305, 311, 674, 820, 878, 915, 1054
FatI CATG 7 cut(s) 301, 307, 670, 816, 874, 911, 1050
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 7 cut(s) 37, 183, 186, 238, 328, 1109, 1191
FokI GGATG 3 cut(s) 156, 785, 1215
Fsp4HI GCNGC 7 cut(s) 37, 183, 186, 238, 328, 1109, 1191
FspBI CTAG 6 cut(s) 23, 234, 252, 521, 978, 1237
GlaI GCGC 1 cut(s) 331
GluI GCNGC 7 cut(s) 37, 183, 186, 238, 328, 1109, 1191
GsaI CCCAGC 2 cut(s) 590, 795
HaeIII GGCC 3 cut(s) 513, 926, 976
HapII CCGG 3 cut(s) 130, 399, 1126
HhaI GCGC 1 cut(s) 332
Hin1II CATG 7 cut(s) 305, 311, 674, 820, 878, 915, 1054
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HindIII AAGCTT 2 cut(s) 292, 1029
HinfI GANTC 4 cut(s) 4, 215, 571, 705
HpaII CCGG 3 cut(s) 130, 399, 1126
HphI GGTGA 3 cut(s) 150, 499, 589
Hpy166II GTNNAC 3 cut(s) 12, 451, 625
Hpy188I TCNGA 5 cut(s) 76, 428, 601, 835, 1057
Hpy188III TCNNGA 3 cut(s) 779, 991, 1025
Hpy8I GTNNAC 3 cut(s) 12, 451, 625
Hpy99I CGWCG 1 cut(s) 1108
HpyAV CCTTC 2 cut(s) 213, 425
HpyCH4III ACNGT 3 cut(s) 487, 855, 1216
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 1108
HpyF3I CTNAG 7 cut(s) 6, 114, 409, 555, 598, 764, 805
Hsp92II CATG 7 cut(s) 305, 311, 674, 820, 878, 915, 1054
HspAI GCGC 1 cut(s) 330
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 2 cut(s) 667, 952
LmnI GCTCC 1 cut(s) 645
Lsp1109I GCAGC 5 cut(s) 23, 194, 314, 1120, 1202
LweI GCATC 5 cut(s) 197, 271, 314, 1059, 1177
MaeI CTAG 6 cut(s) 23, 234, 252, 521, 978, 1237
MaeIII GTNAC 6 cut(s) 156, 498, 558, 615, 1079, 1216
MalI GATC 2 cut(s) 669, 954
MboI GATC 2 cut(s) 667, 952
MboII GAAGA 6 cut(s) 183, 614, 620, 714, 764, 1196
MflI RGATCY 1 cut(s) 952
MhlI GDGCHC 1 cut(s) 14
MluCI AATT 7 cut(s) 42, 108, 264, 403, 723, 768, 1144
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 4 cut(s) 54, 352, 700, 1035
MnlI CCTC 5 cut(s) 725, 812, 979, 1051, 1102
Mph1103I ATGCAT 2 cut(s) 286, 913
MroXI GAANNNNTTC 1 cut(s) 756
MseI TTAA 2 cut(s) 41, 387
MslI CAYNNNNRTG 2 cut(s) 306, 485
MspA1I CMGCKG 2 cut(s) 185, 1097
MspI CCGG 3 cut(s) 130, 399, 1126
MspR9I CCNGG 2 cut(s) 258, 358
Mva1269I GAATGC 1 cut(s) 284
MvaI CCWGG 2 cut(s) 258, 358
MwoI GCNNNNNNNGC 2 cut(s) 45, 1108
NdeII GATC 2 cut(s) 667, 952
NlaIII CATG 7 cut(s) 305, 311, 674, 820, 878, 915, 1054
NlaIV GGNNCC 1 cut(s) 954
NmuCI GTSAC 3 cut(s) 156, 498, 1216
NsiI ATGCAT 2 cut(s) 286, 913
NspI RCATGY 1 cut(s) 305
PaeI GCATGC 1 cut(s) 305
PaqCI CACCTGC 1 cut(s) 187
PcsI WCGNNNNNNNCGW 1 cut(s) 1112
PctI GAATGC 1 cut(s) 284
PdmI GAANNNNTTC 1 cut(s) 756
PfeI GAWTC 2 cut(s) 571, 705
PkrI GCNGC 7 cut(s) 38, 184, 187, 239, 329, 1110, 1192
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Psp6I CCWGG 2 cut(s) 256, 356
PspFI CCCAGC 2 cut(s) 586, 791
PspGI CCWGG 2 cut(s) 256, 356
PspN4I GGNNCC 1 cut(s) 954
PspPI GGNCC 3 cut(s) 354, 443, 924
PstI CTGCAG 2 cut(s) 54, 184
PsuI RGATCY 1 cut(s) 952
RsaI GTAC 2 cut(s) 229, 901
RsaNI GTAC 2 cut(s) 228, 900
RseI CAYNNNNRTG 2 cut(s) 306, 485
SaqAI TTAA 2 cut(s) 41, 387
SatI GCNGC 7 cut(s) 37, 183, 186, 238, 328, 1109, 1191
Sau3AI GATC 2 cut(s) 667, 952
Sau96I GGNCC 3 cut(s) 354, 443, 924
SbfI CCTGCAGG 1 cut(s) 54
ScaI AGTACT 1 cut(s) 229
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 2 cut(s) 258, 358
SdaI CCTGCAGG 1 cut(s) 54
SduI GDGCHC 1 cut(s) 14
SfaNI GCATC 5 cut(s) 197, 271, 314, 1059, 1177
SfcI CTRYAG 2 cut(s) 50, 180
SinI GGWCC 2 cut(s) 354, 443
SmiMI CAYNNNNRTG 2 cut(s) 306, 485
SmlI CTYRAG 2 cut(s) 212, 223
SmoI CTYRAG 2 cut(s) 212, 223
SpeI ACTAGT 1 cut(s) 251
SphI GCATGC 1 cut(s) 305
Sse8387I CCTGCAGG 1 cut(s) 54
Sse9I AATT 7 cut(s) 42, 108, 264, 403, 723, 768, 1144
SsiI CCGC 3 cut(s) 185, 237, 1095
SspI AATATT 1 cut(s) 920
SspMI CTAG 6 cut(s) 23, 234, 252, 521, 978, 1237
StyD4I CCNGG 2 cut(s) 256, 356
StyI CCWWGG 2 cut(s) 514, 927
TaaI ACNGT 3 cut(s) 487, 855, 1216
TaqI TCGA 3 cut(s) 778, 1139, 1173
TasI AATT 7 cut(s) 42, 108, 264, 403, 723, 768, 1144
TatI WGTACW 1 cut(s) 227
TauI GCSGC 2 cut(s) 188, 240
TfiI GAWTC 2 cut(s) 571, 705
Tru1I TTAA 2 cut(s) 41, 387
Tru9I TTAA 2 cut(s) 41, 387
TscAI CASTG 3 cut(s) 14, 572, 977
TseFI GTSAC 3 cut(s) 156, 498, 1216
TseI GCWGC 5 cut(s) 36, 182, 327, 1108, 1190
Tsp45I GTSAC 3 cut(s) 156, 498, 1216
TspDTI ATGAA 3 cut(s) 195, 621, 833
TspGWI ACGGA 1 cut(s) 876
TspRI CASTG 3 cut(s) 14, 572, 977
VneI GTGCAC 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 354, 443
XapI RAATTY 2 cut(s) 264, 1144
XceI RCATGY 1 cut(s) 305
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 624
XmnI GAANNNNTTC 1 cut(s) 756
XspI CTAG 6 cut(s) 23, 234, 252, 521, 978, 1237
ZrmI AGTACT 1 cut(s) 229
Zsp2I ATGCAT 2 cut(s) 286, 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.