Rw2G045300

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0018493
Physical Location & Seq
Forward (+)
72237268 .. 72241084
3817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G045300.1

Sequence Viewer

Length: 2031 bp
ATGAAAATTCTTGCATTATATGCTCCCTATTTTCTCTTACTTTCATGCATACTGCAAAATTACCATTCCATTAAAGCCCAAGTAATTGACAATTTTTTGAACTGTAACGGAACTGTCAATTATACTTCCGGGAGTGTCTATGAGCATAGGTTGAACCTCACCCTCAATTCCCTTGTTGCCAATGCCTCTCTCACAGGATTCTACATGACAAACGTCGGTGACACAAATGACACAGTTTATGGACTTGTTCAATGCAGGGGAGACCTCTCAAAAAATGATTGCAATACTTGTGCAAACACAGCAGCCACAAAGATCAGGCAACTATGTCTAAGCCAGAAGGAGGCTTCCATTGGCTATGTTAATTGTTCATTACAGTATTCCAACCGCCGTTTCTTCTCTACTTTCGACAGCTTTCCCAGATTAAATTTACACAATTACAAGACAGCACCTGATCCGGTTCTTTATAATCGTCAGTTGGGTAACTTGGTTAAGAATCTATCTTCAAAAGCTGCATCTGATCCTTCCAGATTTGCCATTGGGTTCACAAGTTACACAGATTTCATTGATATATATGGTATGGCTCAGTGCACCCAAGACTTGGCAGAGAACAGCTGCTTAATTTGCCTGCAAGAGATTGCTTTTTATATTCCCCAGGATGGAACAGTTGGGGTTACATTGCTTTCTAGGAGTTGCAATCTTCGCTATGAGATATATTCGTTTTCTGTGTCGCCATCAGGCAATCAAACACCTCCAGCAGCATTGCCTCCATCTCCTTTGTTGCTTGAGCCTACACCAGCTACTACTTCTACCACCACTGGTAGTAATACTACTAAAACTAGAAACAAAATTAGTTCTGGAACCATCATTGTCATAGTTATCCCAGCAGTTTTGGCATTGATAGTGATAATAGCAGTCACAACCTGCAGTTGTTACTTGCTGCGGAAAAAGAGTCAATCTGCTACACACCATGATGTTGTTCTTGAAGATGGTAATGATACCATGGATGATGCACTTGTGATTGGACTAAGTGCACTTAAAGTTGCAACAAGCAATTTCTCAGATGCATATAAACTTGGAGAAGGCGGATTTGGTCCAGTTTACAAGGGAAAGCTACCAGATGGACGAGAAATAGCTGTTAAAAGGTTATCAAGTAACTCAGGGCAGGGTCTACAAGAACTAAAAACTGAAGTAGTTCTGGTTGCTAAGTTGCTGCATCGGAATTTGGTGAGGCTGTTAGGCTTCTGCTTAGAAGATGAAGAGAAGCTACTCGTGTATGAGTACCTAAGCAATGGGAGCTTGGACAAAATTTTATTTGATCAGGGGAGAAGATTTGATTTGGAATGGGAAAGACGGTTCAAAATCATTGTTGGAATTGCTAAAGGACTACTTTATCTACATGAAGATTCTCAGCTTAGGATTATTCACCGAGATTTGAAAGCCAGCAATATCCTGTTAGATGAGGACATGAATCCCAAGATATCCGATTTTGGCTTAGCCAAACTGTTCTGTGGTAGCCAGACTCATGGCAATACAAATCGGATATCTGGTACTTTTGGATACATGGCACCTGAATATGCTAAGAATGGGAATTTTTCGACAAAATCAGATGCCTACAGCTTTGGCATTTTAGTTTTGGAAGTTATAACAGGTCGAAAGAACTCTAGCTTCCGCGATTTTTCAAATCTTCAGAGTTATGCATGGCAACATTGGGCAAATGGAACAGCTTTGGATATGTTGGACTCAAGTCTAGGGGATCATCAGTGGCGCAGATTTGAAGTTTTAAAGTGCATCCACATTGGACTATTATGTGTTCAAGAAGCTCCTACTGATAGACCAACCATGTCTGAGGTTTTTATGATGCTAAATAGCTACACTCTTACCTCCCAGGTACCTTCACTTCCAGCATCTTTCGTCCGACAAGGAAGCTCTGCTCACTCGCAGCAGTTGGGGGCTCGCAGGGAAAGTTCTGCTACTTCTGAAGTATCTCAATCAGTAAATGACGTTACAATAACCGAGCTTCATCCTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

676

Amino Acids

74.71

Weight (kDa)

7.17

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 35 - 129 2.8e-20 Salt stress response/antifungal
Stress-antifung PF01657 152 - 237 9.5e-09 Salt stress response/antifungal
Pkinase PF00069 355 - 618 1.5e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 357 - 619 4.5e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018493)

Species Orthologous Gene IDs
malus_domestica MD09G1104200.v1.1 MD17G1091900.v1.1
prunus_persica Prupe.3G221500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0160201
rosa_laevigata RLG00000021204
rosa_rugosa Rorug02G0482400
rosa_samantha Rh2BG561000 Rh2CG531600 Rh2DG569700
rosa_wichuraiana Rw2G045300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 465, 1643
Acc36I ACCTGC 1 cut(s) 929
Acc65I GGTACC 1 cut(s) 1888
AccB1I GGYRCC 2 cut(s) 1564, 1888
AccB7I CCANNNNNTGG 1 cut(s) 598
AccI GTMKAC 1 cut(s) 1168
AccII CGCG 1 cut(s) 1671
AciI CCGC 4 cut(s) 385, 940, 1083, 1669
AclWI GGATC 3 cut(s) 446, 512, 1761
AcsI RAATTY 5 cut(s) 6, 424, 1219, 1305, 1588
AcuI CTGAAG 3 cut(s) 1206, 1670, 1998
AfaI GTAC 3 cut(s) 1280, 1549, 1890
AfiI CCNNNNNNNGG 3 cut(s) 340, 598, 656
AjnI CCWGG 2 cut(s) 651, 1884
AjuI GAANNNNNNNTTGG 4 cut(s) 374, 406, 1071, 1103
AloI GAACNNNNNNTCC 2 cut(s) 1337, 1369
Alw21I GWGCWC 2 cut(s) 590, 1033
Alw26I GTCTC 1 cut(s) 255
Alw44I GTGCAC 2 cut(s) 586, 1029
AlwI GGATC 3 cut(s) 446, 512, 1761
AlwNI CAGNNNCTG 1 cut(s) 449
ApaLI GTGCAC 2 cut(s) 586, 1029
ApeKI GCWGC 7 cut(s) 302, 509, 612, 755, 937, 1210, 1939
ApoI RAATTY 5 cut(s) 6, 424, 1219, 1305, 1588
Asp700I GAANNNNTTC 1 cut(s) 1191
Asp718I GGTACC 1 cut(s) 1888
AspLEI GCGC 1 cut(s) 1767
AspS9I GGNCC 1 cut(s) 1091
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 4 cut(s) 151, 230, 1237, 1415
AvaII GGWCC 1 cut(s) 1091
BaeGI GKGCMC 2 cut(s) 590, 1033
BanI GGYRCC 2 cut(s) 1564, 1888
BanII GRGCYC 1 cut(s) 1954
BarI GAAGNNNNNNTAC 4 cut(s) 1248, 1280, 1881, 1913
BauI CACGAG 1 cut(s) 1268
Bbv12I GWGCWC 2 cut(s) 590, 1033
BbvI GCAGC 7 cut(s) 314, 496, 599, 767, 924, 1197, 1951
BccI CCATC 6 cut(s) 650, 739, 775, 869, 980, 1112
BceAI ACGGC 1 cut(s) 372
BciT130I CCWGG 2 cut(s) 653, 1886
BciVI GTATCC 1 cut(s) 1550
BclI TGATCA 1 cut(s) 1315
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 1 cut(s) 255
BfaI CTAG 4 cut(s) 684, 837, 1662, 1748
BfmI CTRYAG 2 cut(s) 922, 1612
BfuAI ACCTGC 1 cut(s) 929
BfuI GTATCC 1 cut(s) 1550
BisI GCNGC 7 cut(s) 303, 510, 613, 756, 938, 1211, 1940
BlpI GCTNAGC 1 cut(s) 1492
BlsI GCNGC 7 cut(s) 304, 511, 614, 757, 939, 1212, 1941
Bme1390I CCNGG 3 cut(s) 130, 653, 1886
Bme18I GGWCC 1 cut(s) 1091
BmgT120I GGNCC 1 cut(s) 1091
BmiI GGNNCC 3 cut(s) 859, 1566, 1890
BmrFI CCNGG 3 cut(s) 130, 653, 1886
BmsI GCATC 8 cut(s) 521, 997, 1051, 1222, 1597, 1797, 1848, 1913
BoxI GACNNNNGTC 2 cut(s) 212, 1743
BpmI CTGGAG 1 cut(s) 735
Bpu10I CCTNAGC 2 cut(s) 1283, 1412
Bpu1102I GCTNAGC 1 cut(s) 1492
BpuEI CTTGAG 2 cut(s) 803, 1726
BpuMI CCSGG 1 cut(s) 130
BsaI GGTCTC 1 cut(s) 255
BsaJI CCNNGG 3 cut(s) 651, 999, 1884
BsaWI WCCGGW 1 cut(s) 454
Bsc4I CCNNNNNNNGG 3 cut(s) 340, 598, 656
Bse1I ACTGG 2 cut(s) 820, 1094
Bse3DI GCAATG 3 cut(s) 674, 758, 1294
BseBI CCWGG 2 cut(s) 653, 1886
BseDI CCNNGG 3 cut(s) 651, 999, 1884
BseGI GGATG 4 cut(s) 661, 1009, 1788, 2020
BseLI CCNNNNNNNGG 3 cut(s) 340, 598, 656
BseMI GCAATG 3 cut(s) 674, 758, 1294
BseMII CTCAG 5 cut(s) 596, 1071, 1170, 1421, 1836
BseNI ACTGG 2 cut(s) 820, 1094
BseSI GKGCMC 2 cut(s) 590, 1033
BseXI GCAGC 7 cut(s) 314, 496, 599, 767, 924, 1197, 1951
BseYI CCCAGC 1 cut(s) 880
Bsh1236I CGCG 1 cut(s) 1671
BshNI GGYRCC 2 cut(s) 1564, 1888
BsiHKAI GWGCWC 2 cut(s) 590, 1033
BsiSI CCGG 2 cut(s) 129, 455
BslI CCNNNNNNNGG 3 cut(s) 340, 598, 656
BsmAI GTCTC 1 cut(s) 255
Bso31I GGTCTC 1 cut(s) 255
Bsp1286I GDGCHC 3 cut(s) 590, 1033, 1954
Bsp143I GATC 5 cut(s) 312, 451, 517, 1315, 1753
Bsp1720I GCTNAGC 1 cut(s) 1492
Bsp19I CCATGG 1 cut(s) 999
BspACI CCGC 4 cut(s) 385, 940, 1083, 1669
BspCNI CTCAG 5 cut(s) 595, 1070, 1169, 1420, 1837
BspFNI CGCG 1 cut(s) 1671
BspLI GGNNCC 3 cut(s) 859, 1566, 1890
BspMAI CTGCAG 1 cut(s) 926
BspMI ACCTGC 1 cut(s) 929
BspPI GGATC 3 cut(s) 446, 512, 1761
BspT107I GGYRCC 2 cut(s) 1564, 1888
BspTNI GGTCTC 1 cut(s) 255
BsrDI GCAATG 3 cut(s) 674, 758, 1294
BsrI ACTGG 2 cut(s) 820, 1094
BssECI CCNNGG 3 cut(s) 651, 999, 1884
BssMI GATC 5 cut(s) 312, 451, 517, 1315, 1753
BssSI CACGAG 1 cut(s) 1268
BssT1I CCWWGG 1 cut(s) 999
Bst2BI CACGAG 1 cut(s) 1268
Bst2UI CCWGG 2 cut(s) 653, 1886
Bst4CI ACNGT 7 cut(s) 104, 115, 235, 375, 664, 1353, 1503
Bst6I CTCTTC 1 cut(s) 1251
BstAPI GCANNNNNTGC 1 cut(s) 20
BstC8I GCNNGC 3 cut(s) 626, 1441, 1954
BstDSI CCRYGG 1 cut(s) 999
BstF5I GGATG 4 cut(s) 661, 1009, 1788, 2020
BstFNI CGCG 1 cut(s) 1671
BstHHI GCGC 1 cut(s) 1767
BstKTI GATC 5 cut(s) 315, 454, 520, 1318, 1756
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 5 cut(s) 312, 451, 517, 1315, 1753
BstMWI GCNNNNNNNGC 6 cut(s) 20, 299, 621, 699, 890, 1293
BstNI CCWGG 2 cut(s) 653, 1886
BstPAI GACNNNNGTC 2 cut(s) 212, 1743
BstSCI CCNGG 3 cut(s) 128, 651, 1884
BstSFI CTRYAG 2 cut(s) 922, 1612
BstSLI GKGCMC 2 cut(s) 590, 1033
BstUI CGCG 1 cut(s) 1671
BstV1I GCAGC 7 cut(s) 314, 496, 599, 767, 924, 1197, 1951
BstXI CCANNNNNNTGG 1 cut(s) 1523
BsuI GTATCC 1 cut(s) 1550
BtgI CCRYGG 1 cut(s) 999
BtsCI GGATG 4 cut(s) 661, 1009, 1788, 2020
BtsIMutI CAGTG 3 cut(s) 590, 813, 1766
BveI ACCTGC 1 cut(s) 929
Cac8I GCNNGC 3 cut(s) 626, 1441, 1954
CaiI CAGNNNCTG 1 cut(s) 449
CfoI GCGC 1 cut(s) 1767
Cfr13I GGNCC 1 cut(s) 1091
Csp6I GTAC 3 cut(s) 1279, 1548, 1889
CviQI GTAC 3 cut(s) 1279, 1548, 1889
DpnI GATC 5 cut(s) 314, 453, 519, 1317, 1755
DpnII GATC 5 cut(s) 312, 451, 517, 1315, 1753
DraI TTTAAA 1 cut(s) 1782
Eam1104I CTCTTC 1 cut(s) 1251
EarI CTCTTC 1 cut(s) 1251
EciI GGCGGA 1 cut(s) 1098
Eco130I CCWWGG 1 cut(s) 999
Eco24I GRGCYC 1 cut(s) 1954
Eco31I GGTCTC 1 cut(s) 255
Eco32I GATATC 2 cut(s) 1479, 1542
Eco47I GGWCC 1 cut(s) 1091
Eco57I CTGAAG 3 cut(s) 1206, 1670, 1998
EcoRII CCWGG 2 cut(s) 651, 1884
EcoRV GATATC 2 cut(s) 1479, 1542
EcoT14I CCWWGG 1 cut(s) 999
EcoT22I ATGCAT 3 cut(s) 50, 1066, 1699
EcoT38I GRGCYC 1 cut(s) 1954
ErhI CCWWGG 1 cut(s) 999
FalI AAGNNNNNCTT 2 cut(s) 1371, 1403
FbaI TGATCA 1 cut(s) 1315
FblI GTMKAC 1 cut(s) 1168
Fnu4HI GCNGC 7 cut(s) 303, 510, 613, 756, 938, 1211, 1940
FokI GGATG 4 cut(s) 668, 1016, 1775, 2007
FriOI GRGCYC 1 cut(s) 1954
Fsp4HI GCNGC 7 cut(s) 303, 510, 613, 756, 938, 1211, 1940
FspBI CTAG 4 cut(s) 684, 837, 1662, 1748
GlaI GCGC 1 cut(s) 1766
GluI GCNGC 7 cut(s) 303, 510, 613, 756, 938, 1211, 1940
GsaI CCCAGC 1 cut(s) 884
GsuI CTGGAG 1 cut(s) 735
HapII CCGG 2 cut(s) 129, 455
HhaI GCGC 1 cut(s) 1767
Hin6I GCGC 1 cut(s) 1765
HinP1I GCGC 1 cut(s) 1765
HinfI GANTC 7 cut(s) 198, 493, 949, 1403, 1468, 1519, 1739
HpaII CCGG 2 cut(s) 129, 455
HphI GGTGA 4 cut(s) 151, 230, 1237, 1415
Hpy166II GTNNAC 5 cut(s) 543, 588, 1031, 1099, 1169
Hpy188III TCNNGA 4 cut(s) 525, 855, 980, 1814
Hpy8I GTNNAC 5 cut(s) 543, 588, 1031, 1099, 1169
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 4 cut(s) 331, 531, 1073, 1902
HpyCH4III ACNGT 7 cut(s) 104, 115, 235, 375, 664, 1353, 1503
HpyCH4IV ACGT 2 cut(s) 213, 2001
HpyF10VI GCNNNNNNNGC 6 cut(s) 20, 299, 621, 699, 890, 1293
HpySE526I ACGT 2 cut(s) 213, 2001
HspAI GCGC 1 cut(s) 1765
KpnI GGTACC 1 cut(s) 1892
Ksp22I TGATCA 1 cut(s) 1315
Kzo9I GATC 5 cut(s) 312, 451, 517, 1315, 1753
LmnI GCTCC 3 cut(s) 28, 1293, 1825
Lsp1109I GCAGC 7 cut(s) 314, 496, 599, 767, 924, 1197, 1951
LweI GCATC 8 cut(s) 521, 997, 1051, 1222, 1597, 1797, 1848, 1913
MaeI CTAG 4 cut(s) 684, 837, 1662, 1748
MaeII ACGT 2 cut(s) 213, 2001
MaeIII GTNAC 9 cut(s) 104, 218, 479, 548, 670, 913, 929, 1151, 2002
MalI GATC 5 cut(s) 314, 453, 519, 1317, 1755
MboI GATC 5 cut(s) 312, 451, 517, 1315, 1753
MboII GAAGA 9 cut(s) 385, 492, 689, 995, 1262, 1268, 1338, 1412, 1676
MhlI GDGCHC 3 cut(s) 590, 1033, 1954
MlyI GAGTC 3 cut(s) 958, 1513, 1733
MmeI TCCRAC 4 cut(s) 405, 1348, 1716, 1939
Mph1103I ATGCAT 3 cut(s) 50, 1066, 1699
MroXI GAANNNNTTC 1 cut(s) 1191
MseI TTAA 8 cut(s) 72, 360, 422, 489, 617, 1035, 1137, 1781
MslI CAYNNNNRTG 1 cut(s) 969
MspA1I CMGCKG 1 cut(s) 612
MspI CCGG 2 cut(s) 129, 455
MspR9I CCNGG 3 cut(s) 130, 653, 1886
MvaI CCWGG 2 cut(s) 653, 1886
MvnI CGCG 1 cut(s) 1671
MwoI GCNNNNNNNGC 6 cut(s) 20, 299, 621, 699, 890, 1293
NciI CCSGG 1 cut(s) 130
NcoI CCATGG 1 cut(s) 999
NdeII GATC 5 cut(s) 312, 451, 517, 1315, 1753
NlaIV GGNNCC 3 cut(s) 859, 1566, 1890
NmuCI GTSAC 2 cut(s) 218, 913
NsiI ATGCAT 3 cut(s) 50, 1066, 1699
PdmI GAANNNNTTC 1 cut(s) 1191
PfeI GAWTC 4 cut(s) 198, 493, 1403, 1468
PflMI CCANNNNNTGG 1 cut(s) 598
PfoI TCCNGGA 1 cut(s) 128
PkrI GCNGC 7 cut(s) 304, 511, 614, 757, 939, 1212, 1941
PleI GAGTC 3 cut(s) 957, 1513, 1733
PpsI GAGTC 3 cut(s) 957, 1513, 1733
PshAI GACNNNNGTC 2 cut(s) 212, 1743
PsiI TTATAA 2 cut(s) 465, 1643
Psp6I CCWGG 2 cut(s) 651, 1884
PspFI CCCAGC 1 cut(s) 880
PspGI CCWGG 2 cut(s) 651, 1884
PspN4I GGNNCC 3 cut(s) 859, 1566, 1890
PspPI GGNCC 1 cut(s) 1091
PstI CTGCAG 1 cut(s) 926
PstNI CAGNNNCTG 1 cut(s) 449
PvuII CAGCTG 1 cut(s) 612
RsaI GTAC 3 cut(s) 1280, 1549, 1890
RsaNI GTAC 3 cut(s) 1279, 1548, 1889
RseI CAYNNNNRTG 1 cut(s) 969
SaqAI TTAA 8 cut(s) 72, 360, 422, 489, 617, 1035, 1137, 1781
SatI GCNGC 7 cut(s) 303, 510, 613, 756, 938, 1211, 1940
Sau3AI GATC 5 cut(s) 312, 451, 517, 1315, 1753
Sau96I GGNCC 1 cut(s) 1091
SchI GAGTC 3 cut(s) 958, 1513, 1733
ScrFI CCNGG 3 cut(s) 130, 653, 1886
SduI GDGCHC 3 cut(s) 590, 1033, 1954
SfaNI GCATC 8 cut(s) 521, 997, 1051, 1222, 1597, 1797, 1848, 1913
SfcI CTRYAG 2 cut(s) 922, 1612
SinI GGWCC 1 cut(s) 1091
SmiMI CAYNNNNRTG 1 cut(s) 969
SmlI CTYRAG 2 cut(s) 782, 1741
SmoI CTYRAG 2 cut(s) 782, 1741
SsiI CCGC 4 cut(s) 385, 940, 1083, 1669
SspMI CTAG 4 cut(s) 684, 837, 1662, 1748
StyD4I CCNGG 3 cut(s) 128, 651, 1884
StyI CCWWGG 1 cut(s) 999
TaaI ACNGT 7 cut(s) 104, 115, 235, 375, 664, 1353, 1503
TaiI ACGT 2 cut(s) 216, 2004
TaqI TCGA 4 cut(s) 405, 1595, 1651, 2026
TfiI GAWTC 4 cut(s) 198, 493, 1403, 1468
Tru1I TTAA 8 cut(s) 72, 360, 422, 489, 617, 1035, 1137, 1781
Tru9I TTAA 8 cut(s) 72, 360, 422, 489, 617, 1035, 1137, 1781
TscAI CASTG 3 cut(s) 590, 820, 1766
TseFI GTSAC 2 cut(s) 218, 913
TseI GCWGC 7 cut(s) 302, 509, 612, 755, 937, 1210, 1939
Tsp45I GTSAC 2 cut(s) 218, 913
TspDTI ATGAA 8 cut(s) 17, 33, 357, 550, 1269, 1413, 1481, 2009
TspGWI ACGGA 1 cut(s) 123
TspRI CASTG 3 cut(s) 590, 820, 1766
Van91I CCANNNNNTGG 1 cut(s) 598
VneI GTGCAC 2 cut(s) 586, 1029
VpaK11BI GGWCC 1 cut(s) 1091
XapI RAATTY 5 cut(s) 6, 424, 1219, 1305, 1588
XmiI GTMKAC 1 cut(s) 1168
XmnI GAANNNNTTC 1 cut(s) 1191
XspI CTAG 4 cut(s) 684, 837, 1662, 1748
Zsp2I ATGCAT 3 cut(s) 50, 1066, 1699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.