MD17G1125600.v1.1
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0018346
Physical Location & Seq
Reverse (-)
10924910 .. 10925656
747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1125600.v1.1.491

Sequence Viewer

Length: 438 bp
ATGCATTCTGAGCTTGACGCACTTGAAGCCAATCACACCTGGACTTTGGTTCCTTTACCCCTCCACCAACGCCCCATTGGATGTAAATGGGTCTTCAAAATCAAGTATCATTCCGACGGTACCATCGAACGCTACAAGGCTCGCCTTGTCGCCAAAGGGTTCACGCAGCGCGAAGCTGGTCTCTTCACCAAATGGATGTCCAAAACGCTTTCCTTCACGGTGCACTCCATGAGGAAGTATATATGTTACCCCCTCCTGGTTATTGTCGACAGGGGGAGACTATGGTTTGTCGACTTCACAAGTCATTATACGGACTCAAGCAGGCATCTCGCAGCTGGTTCCAACGTTTTTCATCAACCATTCAAGAAGTTGGTTTTCAACAGTCTCATGCAGACTACTCATTATTCACTAAAGTTTGTGGATACTCCATTACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag

Protein Analysis

146

Amino Acids

16.97

Weight (kDa)

9.76

Isoelectric Point (pI)

26.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 11 - 67 5.8e-12 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09832
malus_domestica MD17G1125600.v1.1
pyrus_communis pycom07g08290 pycom08g03650 pycom10g05700 pycom13g21050 pycom17g16760
rosa_wichuraiana Rw1G014600 Rw4G005310 Rw4G016610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 119
AccI GTMKAC 2 cut(s) 267, 291
AccII CGCG 1 cut(s) 171
AclI AACGTT 1 cut(s) 345
AfaI GTAC 1 cut(s) 121
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 4 cut(s) 26, 97, 364, 379
AjnI CCWGG 2 cut(s) 38, 255
AluBI AGCT 3 cut(s) 13, 176, 335
AluI AGCT 3 cut(s) 13, 176, 335
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 3 cut(s) 185, 271, 389
Alw44I GTGCAC 1 cut(s) 221
ApaLI GTGCAC 1 cut(s) 221
ApeKI GCWGC 2 cut(s) 166, 332
Asp718I GGTACC 1 cut(s) 119
AspLEI GCGC 1 cut(s) 171
AsuHPI GGTGA 1 cut(s) 178
BaeGI GKGCMC 1 cut(s) 225
BanI GGYRCC 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 178, 344
BccI CCATC 1 cut(s) 131
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 2 cut(s) 40, 257
BciVI GTATCC 1 cut(s) 415
BcoDI GTCTC 3 cut(s) 185, 271, 389
BfmI CTRYAG 1 cut(s) 434
BfuI GTATCC 1 cut(s) 415
BisI GCNGC 2 cut(s) 167, 333
BlsI GCNGC 2 cut(s) 168, 334
Bme1390I CCNGG 2 cut(s) 40, 257
BmiI GGNNCC 3 cut(s) 51, 121, 340
BmrFI CCNGG 2 cut(s) 40, 257
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 1 cut(s) 85
BpuEI CTTGAG 1 cut(s) 301
BsaI GGTCTC 1 cut(s) 185
Bsc4I CCNNNNNNNGG 1 cut(s) 256
BseBI CCWGG 2 cut(s) 40, 257
BseGI GGATG 2 cut(s) 86, 201
BseLI CCNNNNNNNGG 1 cut(s) 256
BseSI GKGCMC 1 cut(s) 225
BseXI GCAGC 2 cut(s) 178, 344
Bsh1236I CGCG 1 cut(s) 171
BshNI GGYRCC 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 225
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 3 cut(s) 185, 271, 389
BsmI GAATGC 1 cut(s) 4
Bso31I GGTCTC 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 225
BspFNI CGCG 1 cut(s) 171
BspLI GGNNCC 3 cut(s) 51, 121, 340
BspT107I GGYRCC 1 cut(s) 119
BspTNI GGTCTC 1 cut(s) 185
Bst2UI CCWGG 2 cut(s) 40, 257
Bst4CI ACNGT 4 cut(s) 119, 220, 383, 435
Bst6I CTCTTC 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 142, 323
BstDEI CTNAG 1 cut(s) 9
BstF5I GGATG 2 cut(s) 86, 201
BstFNI CGCG 1 cut(s) 171
BstHHI GCGC 1 cut(s) 171
BstMAI GTCTC 3 cut(s) 185, 271, 389
BstMWI GCNNNNNNNGC 2 cut(s) 10, 26
BstNI CCWGG 2 cut(s) 40, 257
BstSCI CCNGG 2 cut(s) 38, 255
BstSFI CTRYAG 1 cut(s) 434
BstSLI GKGCMC 1 cut(s) 225
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 178, 344
BstV2I GAAGAC 1 cut(s) 85
BsuI GTATCC 1 cut(s) 415
BtsCI GGATG 2 cut(s) 86, 201
Cac8I GCNNGC 2 cut(s) 142, 323
CfoI GCGC 1 cut(s) 171
CseI GACGC 1 cut(s) 26
Csp6I GTAC 1 cut(s) 120
CviAII CATG 2 cut(s) 229, 388
CviJI RGCY 5 cut(s) 13, 29, 140, 176, 335
CviKI_1 RGCY 5 cut(s) 13, 29, 140, 176, 335
CviQI GTAC 1 cut(s) 120
DdeI CTNAG 1 cut(s) 9
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 38, 255
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 232, 391
FaiI YATR 7 cut(s) 230, 240, 242, 244, 283, 309, 389
FatI CATG 2 cut(s) 228, 387
FblI GTMKAC 2 cut(s) 267, 291
Fnu4HI GCNGC 2 cut(s) 167, 333
FokI GGATG 2 cut(s) 93, 208
Fsp4HI GCNGC 2 cut(s) 167, 333
GlaI GCGC 1 cut(s) 170
GluI GCNGC 2 cut(s) 167, 333
HgaI GACGC 1 cut(s) 26
HhaI GCGC 1 cut(s) 171
Hin1II CATG 2 cut(s) 232, 391
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 2 cut(s) 268, 292
HindII GTYRAC 2 cut(s) 268, 292
HinfI GANTC 1 cut(s) 314
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 4 cut(s) 162, 223, 268, 292
Hpy188I TCNGA 2 cut(s) 10, 115
Hpy188III TCNNGA 1 cut(s) 364
Hpy8I GTNNAC 4 cut(s) 162, 223, 268, 292
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 1 cut(s) 223
HpyCH4III ACNGT 4 cut(s) 119, 220, 383, 435
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 3 cut(s) 4, 223, 391
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 26
HpyF3I CTNAG 1 cut(s) 9
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 2 cut(s) 232, 391
HspAI GCGC 1 cut(s) 169
KpnI GGTACC 1 cut(s) 123
LpnPI CCDG 8 cut(s) 25, 52, 162, 242, 256, 269, 307, 321
Lsp1109I GCAGC 2 cut(s) 178, 344
LweI GCATC 1 cut(s) 334
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 245
MboII GAAGA 2 cut(s) 85, 175
MhlI GDGCHC 1 cut(s) 225
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 2 cut(s) 138, 366
MnlI CCTC 3 cut(s) 71, 225, 263
Mph1103I ATGCAT 1 cut(s) 6
MspA1I CMGCKG 1 cut(s) 335
MspR9I CCNGG 2 cut(s) 40, 257
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 2 cut(s) 40, 257
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 10, 26
NlaIII CATG 2 cut(s) 232, 391
NlaIV GGNNCC 3 cut(s) 51, 121, 340
NsiI ATGCAT 1 cut(s) 6
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 1 cut(s) 4
PkrI GCNGC 2 cut(s) 168, 334
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Psp1406I AACGTT 1 cut(s) 345
Psp6I CCWGG 2 cut(s) 38, 255
PspGI CCWGG 2 cut(s) 38, 255
PspN4I GGNNCC 3 cut(s) 51, 121, 340
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 121
RsaNI GTAC 1 cut(s) 120
SalI GTCGAC 2 cut(s) 266, 290
SatI GCNGC 2 cut(s) 167, 333
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 2 cut(s) 40, 257
SduI GDGCHC 1 cut(s) 225
SetI ASST 5 cut(s) 15, 41, 178, 337, 348
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 434
SmlI CTYRAG 1 cut(s) 316
SmoI CTYRAG 1 cut(s) 316
StyD4I CCNGG 2 cut(s) 38, 255
TaaI ACNGT 4 cut(s) 119, 220, 383, 435
TaiI ACGT 1 cut(s) 348
TaqI TCGA 3 cut(s) 126, 267, 291
TseI GCWGC 2 cut(s) 166, 332
TspDTI ATGAA 1 cut(s) 341
TspGWI ACGGA 1 cut(s) 326
VneI GTGCAC 1 cut(s) 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XmiI GTMKAC 2 cut(s) 267, 291
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.