Rw4G016610

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018346
Physical Location & Seq
Reverse (-)
37802054 .. 37802575
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G016610.1

Sequence Viewer

Length: 522 bp
ATGGTAGAACTCAAGGAAGTGTCCAACGAGCCACAAGTTAAAACTTCAATTCCCGATGAACAACTTGCTAAACCAACTATAACACAAACTCCAAGAAGATCTAGTAGGATCCGGATACCTCCTAATAAGTATGGTTTGTGTCATGAAAACCTTGGGGAGTTAAATCTCCTTGGTGATAATGAAAACATGCATCATCCTTCTAGTTATAATGAAGCAATGTTAGACATTGACTCAAAGAAATGGCAAGAAGCCATGGCATCCGAGATTGACTCTATGTATACCAACCAAGTCTGGACTCTCGTTGACCCTCCACCTGGTATTAGACCAATTGGAAACAAATGGGTCTTCAAGAAAAAGCTAGGCTCAGATGGTAATATAGAAACCTACAAGGCTAGGTTTATGGCAAAGGGCTATAAGCAAAGAGAGGGCATTGACTATGAAGATACGTTCTCTCCTATTGCCATGGTTAAATCCATTCGGATATTTTTTGCTATAGCTGCTCACTATGATTATGAGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2

Protein Analysis

173

Amino Acids

19.87

Weight (kDa)

6.1

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 95 - 172 3.3e-21 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09832
malus_domestica MD17G1125600.v1.1
pyrus_communis pycom07g08290 pycom08g03650 pycom10g05700 pycom13g21050 pycom17g16760
rosa_wichuraiana Rw1G014600 Rw4G005310 Rw4G016610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
AccI GTMKAC 1 cut(s) 278
AccIII TCCGGA 1 cut(s) 111
AclWI GGATC 2 cut(s) 103, 116
AfiI CCNNNNNNNGG 1 cut(s) 314
AgsI TTSAA 2 cut(s) 48, 349
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 2 cut(s) 358, 497
AluI AGCT 2 cut(s) 358, 497
AlwI GGATC 2 cut(s) 103, 116
Aor13HI TCCGGA 1 cut(s) 111
ApeKI GCWGC 1 cut(s) 497
AsuHPI GGTGA 1 cut(s) 185
BamHI GGATCC 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 337
BbvI GCAGC 1 cut(s) 484
BccI CCATC 1 cut(s) 362
BciT130I CCWGG 1 cut(s) 315
BciVI GTATCC 1 cut(s) 108
BfaI CTAG 4 cut(s) 102, 201, 359, 393
BfmI CTRYAG 1 cut(s) 492
BfuI GTATCC 1 cut(s) 108
BglII AGATCT 1 cut(s) 98
BisI GCNGC 1 cut(s) 498
BlsI GCNGC 1 cut(s) 499
Bme1390I CCNGG 1 cut(s) 315
BmiI GGNNCC 1 cut(s) 110
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 2 cut(s) 199, 266
BpiI GAAGAC 1 cut(s) 337
BplI GAGNNNNNCTC 2 cut(s) 254, 286
BsaJI CCNNGG 4 cut(s) 151, 169, 252, 462
BsaWI WCCGGW 1 cut(s) 111
BsaXI ACNNNNNCTCC 4 cut(s) 73, 103, 436, 466
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse3DI GCAATG 1 cut(s) 222
BseAI TCCGGA 1 cut(s) 111
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 4 cut(s) 151, 169, 252, 462
BseGI GGATG 2 cut(s) 193, 257
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 1 cut(s) 378
BseXI GCAGC 1 cut(s) 484
BsiSI CCGG 1 cut(s) 112
BslI CCNNNNNNNGG 1 cut(s) 314
Bsp13I TCCGGA 1 cut(s) 111
Bsp143I GATC 2 cut(s) 98, 108
Bsp19I CCATGG 2 cut(s) 252, 462
BspCNI CTCAG 1 cut(s) 377
BspEI TCCGGA 1 cut(s) 111
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 110
BspPI GGATC 2 cut(s) 103, 116
BsrDI GCAATG 1 cut(s) 222
BssECI CCNNGG 4 cut(s) 151, 169, 252, 462
BssMI GATC 2 cut(s) 98, 108
BssNAI GTATAC 1 cut(s) 279
BssT1I CCWWGG 4 cut(s) 151, 169, 252, 462
Bst1107I GTATAC 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 315
BstDEI CTNAG 1 cut(s) 364
BstDSI CCRYGG 2 cut(s) 252, 462
BstF5I GGATG 2 cut(s) 193, 257
BstKTI GATC 2 cut(s) 101, 111
BstMBI GATC 2 cut(s) 98, 108
BstMWI GCNNNNNNNGC 1 cut(s) 497
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 1 cut(s) 190
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 492
BstV1I GCAGC 1 cut(s) 484
BstV2I GAAGAC 1 cut(s) 337
BstX2I RGATCY 2 cut(s) 98, 108
BstYI RGATCY 2 cut(s) 98, 108
BstZ17I GTATAC 1 cut(s) 279
BsuI GTATCC 1 cut(s) 108
BtgI CCRYGG 2 cut(s) 252, 462
BtsCI GGATG 2 cut(s) 193, 257
CciI TCATGA 1 cut(s) 142
CsiI ACCWGGT 1 cut(s) 313
CviAII CATG 4 cut(s) 143, 187, 253, 463
CviJI RGCY 7 cut(s) 31, 251, 358, 363, 392, 411, 497
CviKI_1 RGCY 7 cut(s) 31, 251, 358, 363, 392, 411, 497
DdeI CTNAG 1 cut(s) 364
DpnI GATC 2 cut(s) 100, 110
DpnII GATC 2 cut(s) 98, 108
Eco130I CCWWGG 4 cut(s) 151, 169, 252, 462
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 4 cut(s) 151, 169, 252, 462
EcoT22I ATGCAT 1 cut(s) 192
ErhI CCWWGG 4 cut(s) 151, 169, 252, 462
FaeI CATG 4 cut(s) 146, 190, 256, 466
FatI CATG 4 cut(s) 142, 186, 252, 462
FblI GTMKAC 1 cut(s) 278
Fnu4HI GCNGC 1 cut(s) 498
FokI GGATG 2 cut(s) 180, 244
Fsp4HI GCNGC 1 cut(s) 498
FspBI CTAG 4 cut(s) 102, 201, 359, 393
GluI GCNGC 1 cut(s) 498
HapII CCGG 1 cut(s) 112
Hin1II CATG 4 cut(s) 146, 190, 256, 466
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 3 cut(s) 230, 269, 295
HpaII CCGG 1 cut(s) 112
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 2 cut(s) 279, 304
Hpy188I TCNGA 3 cut(s) 262, 367, 480
Hpy188III TCNNGA 5 cut(s) 53, 112, 143, 292, 349
Hpy8I GTNNAC 2 cut(s) 279, 304
HpyAV CCTTC 1 cut(s) 207
HpyCH4IV ACGT 1 cut(s) 446
HpyCH4V TGCA 1 cut(s) 190
HpyF10VI GCNNNNNNNGC 1 cut(s) 497
HpyF3I CTNAG 1 cut(s) 364
HpySE526I ACGT 1 cut(s) 446
Hsp92II CATG 4 cut(s) 146, 190, 256, 466
Kpn2I TCCGGA 1 cut(s) 111
Kzo9I GATC 2 cut(s) 98, 108
LpnPI CCDG 4 cut(s) 125, 277, 300, 327
Lsp1109I GCAGC 1 cut(s) 484
LweI GCATC 2 cut(s) 199, 266
MabI ACCWGGT 1 cut(s) 313
MaeI CTAG 4 cut(s) 102, 201, 359, 393
MaeII ACGT 1 cut(s) 446
MalI GATC 2 cut(s) 100, 110
MboI GATC 2 cut(s) 98, 108
MboII GAAGA 3 cut(s) 108, 337, 452
MfeI CAATTG 1 cut(s) 327
MflI RGATCY 2 cut(s) 98, 108
MluCI AATT 2 cut(s) 48, 327
MlyI GAGTC 3 cut(s) 224, 263, 289
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 3 cut(s) 129, 318, 418
Mph1103I ATGCAT 1 cut(s) 192
MroI TCCGGA 1 cut(s) 111
MseI TTAA 3 cut(s) 39, 161, 468
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 1 cut(s) 315
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 497
NcoI CCATGG 2 cut(s) 252, 462
NdeII GATC 2 cut(s) 98, 108
NlaIII CATG 4 cut(s) 146, 190, 256, 466
NlaIV GGNNCC 1 cut(s) 110
NsiI ATGCAT 1 cut(s) 192
NspI RCATGY 1 cut(s) 190
PagI TCATGA 1 cut(s) 142
PkrI GCNGC 1 cut(s) 499
PleI GAGTC 3 cut(s) 224, 263, 289
PpsI GAGTC 3 cut(s) 224, 263, 289
PsiI TTATAA 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 110
PsuI RGATCY 2 cut(s) 98, 108
SaqAI TTAA 3 cut(s) 39, 161, 468
SatI GCNGC 1 cut(s) 498
Sau3AI GATC 2 cut(s) 98, 108
SchI GAGTC 3 cut(s) 224, 263, 289
ScrFI CCNGG 1 cut(s) 315
SetI ASST 8 cut(s) 121, 153, 316, 360, 386, 398, 449, 499
SexAI ACCWGGT 1 cut(s) 313
SfaNI GCATC 2 cut(s) 199, 266
SfcI CTRYAG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
Sse9I AATT 2 cut(s) 48, 327
SspMI CTAG 4 cut(s) 102, 201, 359, 393
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 4 cut(s) 151, 169, 252, 462
TaiI ACGT 1 cut(s) 449
TasI AATT 2 cut(s) 48, 327
Tru1I TTAA 3 cut(s) 39, 161, 468
Tru9I TTAA 3 cut(s) 39, 161, 468
TseI GCWGC 1 cut(s) 497
TspDTI ATGAA 5 cut(s) 72, 159, 195, 225, 453
XceI RCATGY 1 cut(s) 190
XmiI GTMKAC 1 cut(s) 278
XspI CTAG 4 cut(s) 102, 201, 359, 393
Zsp2I ATGCAT 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.