MD17G1131300.v1.1

N-acetyl-D-glucosamine kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010229
Physical Location & Seq
Forward (+)
11582107 .. 11586226
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1131300.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGAAGCGCTACAGGAACGGAGAAATCTGGGACTTCGAGCACCAAATGCCGGTGGCCGCCGGCGCTGGCGGTGTTATATTGGGTTTGGACGGCGGAACCACCTCCACCGTCTGCATTTGCATGCCGATTTTGCCCTTCTCCGACCCTTTCCCCGACCCGGTTCCCGTCCTCGCTCGAGCTGTCGCTGGCTGCACCAACCACAACAGCGTTGGCGAGGCCGCTGCGAGGGAAACATTGGAGCAGGTGATGGCGGAAGCGTTGGCCAATTCTGGTTCGAACCGATCGGTGGTTCGGGCTGTTTGTTTGGCGATTTCCGGTGTCAACCACCCCACTGATCAGCAGAGGATATTGGATTGGCTGAGGGATATATTTCCCAGCCATGTGAGGTTGTATGTTCAGAATGATGCTGTTGCTGCTCTAGCTTGTGGGACTTTGGGGAAGCTTCATGGGTGTGTTCTAATTGCTGGCACGGGGACCATTGCGTACGGGTTCACAGAAGACGGTAGAGAAGCTCGGGCAGCCGGTGCAGGTCCTACCTTAGGTGATTGGGGAAGTGGATACGGTATAGCTGCACAGGCATTAACCGCTGTGGTAAGGGCCAACGATGGTCGTGGTCCAGATACAAAGCTTACATCTAGTATTTTAGAGGAGCTTGGTCTTTCTTCTCCAGATGAACTCATCGGGTGGACGTATGCAGACCCATCATGGGCTCGCATTGCAGCACTTGTTCCAGTTGTTGTTTCTTGTGCAGAGGCTGGTGATGAAGTTGCGAATAAGATTCTGTTTGATTCAGTCCAGGAGTTAGGGCTGAGTGTGAAAGCTGTTGTTCAAAGACTTGGCTTGTGTGGTCCAGAGGGAAAAGATTCTTTTCCGCTCGTCATGGTTGGTGGTGTTCTTGAAGCAAACAAAAGGTGGGATATAGGAACGGAAGTCATCAAATGCATCTCCAAGGGCTACCCAGGGGCAGTTCCAATTAGGCCTAAGGTTGAGCCTGCAGTTGGAGCAGCATTGCTAGCATGGAATTTTTGCATGAAAGAATCTCTGAAGGAAGCCTTCAAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BcrAD_BadFG

Protein Analysis

355

Amino Acids

37.22

Weight (kDa)

5.37

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BcrAD_BadFG PF01869 27 - 339 2.3e-58 BadF/BadG/BcrA/BcrD ATPase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 232
Acc36I ACCTGC 2 cut(s) 232, 518
AccBSI CCGCTC 1 cut(s) 874
AciI CCGC 7 cut(s) 57, 69, 93, 219, 251, 585, 872
AcoI YGGCCR 2 cut(s) 54, 261
AcsI RAATTY 1 cut(s) 1021
AcuI CTGAAG 1 cut(s) 1064
AfaI GTAC 1 cut(s) 485
AfeI AGCGCT 1 cut(s) 8
AfiI CCNNNNNNNGG 8 cut(s) 49, 157, 225, 286, 539, 706, 998, 1059
AgsI TTSAA 3 cut(s) 830, 899, 1057
AjnI CCWGG 2 cut(s) 795, 958
AluBI AGCT 8 cut(s) 179, 422, 442, 512, 569, 628, 652, 821
AluI AGCT 8 cut(s) 179, 422, 442, 512, 569, 628, 652, 821
Alw21I GWGCWC 1 cut(s) 42
AlwNI CAGNNNCTG 1 cut(s) 755
Ama87I CYCGRG 2 cut(s) 174, 513
Aor51HI AGCGCT 1 cut(s) 8
AoxI GGCC 5 cut(s) 54, 216, 261, 597, 977
ApeKI GCWGC 7 cut(s) 189, 221, 413, 518, 569, 719, 1004
ApoI RAATTY 1 cut(s) 1021
Asp700I GAANNNNTTC 1 cut(s) 862
AspLEI GCGC 2 cut(s) 9, 65
AspS9I GGNCC 5 cut(s) 474, 530, 597, 614, 848
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 3 cut(s) 256, 554, 770
AsuII TTCGAA 1 cut(s) 275
AsuNHI GCTAGC 1 cut(s) 1012
AvaI CYCGRG 2 cut(s) 174, 513
AvaII GGWCC 4 cut(s) 474, 530, 614, 848
AxyI CCTNAGG 2 cut(s) 538, 981
BalI TGGCCA 1 cut(s) 263
BanII GRGCYC 1 cut(s) 712
BbsI GAAGAC 1 cut(s) 504
Bbv12I GWGCWC 1 cut(s) 42
BbvCI CCTCAGC 1 cut(s) 359
BbvI GCAGC 7 cut(s) 176, 208, 400, 530, 556, 731, 1016
BccI CCATC 3 cut(s) 241, 599, 709
BceAI ACGGC 1 cut(s) 106
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BciT130I CCWGG 2 cut(s) 797, 960
BciVI GTATCC 1 cut(s) 551
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 158
BfaI CTAG 3 cut(s) 419, 636, 1013
BfmI CTRYAG 2 cut(s) 10, 993
BfoI RGCGCY 2 cut(s) 10, 66
BfuAI ACCTGC 2 cut(s) 232, 518
BfuI GTATCC 1 cut(s) 551
BisI GCNGC 9 cut(s) 57, 190, 219, 222, 414, 519, 570, 720, 1005
BlsI GCNGC 9 cut(s) 58, 191, 220, 223, 415, 520, 571, 721, 1006
Bme1390I CCNGG 3 cut(s) 158, 797, 960
Bme18I GGWCC 4 cut(s) 474, 530, 614, 848
BmeT110I CYCGRG 2 cut(s) 174, 513
BmgT120I GGNCC 5 cut(s) 474, 530, 597, 614, 848
BmiI GGNNCC 3 cut(s) 97, 162, 475
BmrFI CCNGG 3 cut(s) 158, 797, 960
BmsI GCATC 2 cut(s) 394, 951
BmtI GCTAGC 1 cut(s) 1016
BpiI GAAGAC 1 cut(s) 504
BpmI CTGGAG 1 cut(s) 651
Bpu10I CCTNAGC 1 cut(s) 359
Bpu14I TTCGAA 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 158
BsaJI CCNNGG 3 cut(s) 948, 958, 959
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 8 cut(s) 49, 157, 225, 286, 539, 706, 998, 1059
Bse118I RCCGGY 3 cut(s) 49, 59, 521
Bse1I ACTGG 1 cut(s) 731
Bse21I CCTNAGG 2 cut(s) 538, 981
Bse3DI GCAATG 3 cut(s) 477, 714, 1007
BseBI CCWGG 2 cut(s) 797, 960
BseDI CCNNGG 3 cut(s) 948, 958, 959
BseLI CCNNNNNNNGG 8 cut(s) 49, 157, 225, 286, 539, 706, 998, 1059
BseMI GCAATG 3 cut(s) 477, 714, 1007
BseMII CTCAG 2 cut(s) 350, 800
BseNI ACTGG 1 cut(s) 731
BseRI GAGGAG 1 cut(s) 662
BseXI GCAGC 7 cut(s) 176, 208, 400, 530, 556, 731, 1016
BseYI CCCAGC 1 cut(s) 374
BsgI GTGCAG 4 cut(s) 175, 546, 555, 768
Bsh1285I CGRYCG 1 cut(s) 284
BshFI GGCC 5 cut(s) 56, 218, 263, 599, 979
BsiEI CGRYCG 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 42
BsiHKCI CYCGRG 2 cut(s) 174, 513
BsiSI CCGG 5 cut(s) 50, 60, 158, 315, 522
BsiWI CGTACG 1 cut(s) 483
BslFI GGGAC 3 cut(s) 44, 442, 487
BslI CCNNNNNNNGG 8 cut(s) 49, 157, 225, 286, 539, 706, 998, 1059
BsmFI GGGAC 3 cut(s) 44, 442, 487
BsnI GGCC 5 cut(s) 56, 218, 263, 599, 979
BsoBI CYCGRG 2 cut(s) 174, 513
Bsp119I TTCGAA 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 42, 712
Bsp143I GATC 2 cut(s) 281, 334
BspACI CCGC 7 cut(s) 57, 69, 93, 219, 251, 585, 872
BspANI GGCC 5 cut(s) 56, 218, 263, 599, 979
BspCNI CTCAG 2 cut(s) 351, 801
BspLI GGNNCC 3 cut(s) 97, 162, 475
BspMAI CTGCAG 1 cut(s) 997
BspMI ACCTGC 2 cut(s) 232, 518
BspOI GCTAGC 1 cut(s) 1016
BspT104I TTCGAA 1 cut(s) 275
BsrBI CCGCTC 1 cut(s) 874
BsrDI GCAATG 3 cut(s) 477, 714, 1007
BsrFI RCCGGY 3 cut(s) 49, 59, 521
BsrI ACTGG 1 cut(s) 731
BssAI RCCGGY 3 cut(s) 49, 59, 521
BssECI CCNNGG 3 cut(s) 948, 958, 959
BssMI GATC 2 cut(s) 281, 334
BssT1I CCWWGG 1 cut(s) 948
Bst2UI CCWGG 2 cut(s) 797, 960
Bst4CI ACNGT 3 cut(s) 109, 503, 563
BstAPI GCANNNNNTGC 2 cut(s) 46, 524
BstBI TTCGAA 1 cut(s) 275
BstC8I GCNNGC 8 cut(s) 61, 67, 122, 187, 466, 712, 993, 1014
BstDEI CTNAG 4 cut(s) 359, 538, 809, 981
BstENI CCTNNNNNAGG 2 cut(s) 537, 1057
BstH2I RGCGCY 2 cut(s) 10, 66
BstHHI GCGC 2 cut(s) 9, 65
BstKTI GATC 2 cut(s) 284, 337
BstMBI GATC 2 cut(s) 281, 334
BstMCI CGRYCG 1 cut(s) 284
BstNI CCWGG 2 cut(s) 797, 960
BstNSI RCATGY 1 cut(s) 124
BstSCI CCNGG 3 cut(s) 156, 795, 958
BstSFI CTRYAG 2 cut(s) 10, 993
BstV1I GCAGC 7 cut(s) 176, 208, 400, 530, 556, 731, 1016
BstV2I GAAGAC 1 cut(s) 504
Bsu36I CCTNAGG 2 cut(s) 538, 981
BsuI GTATCC 1 cut(s) 551
BsuRI GGCC 5 cut(s) 56, 218, 263, 599, 979
BtsIMutI CAGTG 1 cut(s) 330
BveI ACCTGC 2 cut(s) 232, 518
Cac8I GCNNGC 8 cut(s) 61, 67, 122, 187, 466, 712, 993, 1014
CaiI CAGNNNCTG 1 cut(s) 755
CfoI GCGC 2 cut(s) 9, 65
Cfr10I RCCGGY 3 cut(s) 49, 59, 521
Cfr13I GGNCC 5 cut(s) 474, 530, 597, 614, 848
Csp6I GTAC 1 cut(s) 484
CviAII CATG 7 cut(s) 121, 380, 446, 705, 880, 1017, 1030
CviQI GTAC 1 cut(s) 484
DdeI CTNAG 4 cut(s) 359, 538, 809, 981
DpnI GATC 2 cut(s) 283, 336
DpnII GATC 2 cut(s) 281, 334
EaeI YGGCCR 2 cut(s) 54, 261
EciI GGCGGA 2 cut(s) 108, 266
Eco130I CCWWGG 1 cut(s) 948
Eco147I AGGCCT 1 cut(s) 979
Eco24I GRGCYC 1 cut(s) 712
Eco47I GGWCC 4 cut(s) 474, 530, 614, 848
Eco47III AGCGCT 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 1064
Eco81I CCTNAGG 2 cut(s) 538, 981
Eco88I CYCGRG 2 cut(s) 174, 513
EcoNI CCTNNNNNAGG 2 cut(s) 537, 1057
EcoO109I RGGNCCY 1 cut(s) 530
EcoRII CCWGG 2 cut(s) 795, 958
EcoT14I CCWWGG 1 cut(s) 948
EcoT22I ATGCAT 1 cut(s) 944
EcoT38I GRGCYC 1 cut(s) 712
ErhI CCWWGG 1 cut(s) 948
FaeI CATG 7 cut(s) 124, 383, 449, 708, 883, 1020, 1033
FalI AAGNNNNNCTT 1 cut(s) 1037
FaqI GGGAC 3 cut(s) 44, 442, 487
FatI CATG 7 cut(s) 120, 379, 445, 704, 879, 1016, 1029
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 9 cut(s) 57, 190, 219, 222, 414, 519, 570, 720, 1005
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 9 cut(s) 57, 190, 219, 222, 414, 519, 570, 720, 1005
FspBI CTAG 3 cut(s) 419, 636, 1013
GlaI GCGC 2 cut(s) 8, 64
GluI GCNGC 9 cut(s) 57, 190, 219, 222, 414, 519, 570, 720, 1005
GsaI CCCAGC 1 cut(s) 378
GsuI CTGGAG 1 cut(s) 651
HaeII RGCGCY 2 cut(s) 10, 66
HaeIII GGCC 5 cut(s) 56, 218, 263, 599, 979
HapII CCGG 5 cut(s) 50, 60, 158, 315, 522
HhaI GCGC 2 cut(s) 9, 65
Hin1II CATG 7 cut(s) 124, 383, 449, 708, 883, 1020, 1033
Hin6I GCGC 2 cut(s) 7, 63
HinP1I GCGC 2 cut(s) 7, 63
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 2 cut(s) 440, 626
HinfI GANTC 4 cut(s) 778, 788, 863, 1037
HpaII CCGG 5 cut(s) 50, 60, 158, 315, 522
HphI GGTGA 3 cut(s) 256, 554, 770
Hpy166II GTNNAC 3 cut(s) 322, 492, 687
Hpy188I TCNGA 3 cut(s) 142, 399, 1044
Hpy188III TCNNGA 5 cut(s) 617, 668, 851, 896, 1057
Hpy8I GTNNAC 3 cut(s) 322, 492, 687
HpyAV CCTTC 3 cut(s) 145, 1039, 1063
HpyCH4III ACNGT 3 cut(s) 109, 503, 563
HpyCH4IV ACGT 1 cut(s) 689
HpyF3I CTNAG 4 cut(s) 359, 538, 809, 981
HpySE526I ACGT 1 cut(s) 689
Hsp92II CATG 7 cut(s) 124, 383, 449, 708, 883, 1020, 1033
HspAI GCGC 2 cut(s) 7, 63
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 281, 334
LmnI GCTCC 3 cut(s) 238, 649, 1001
Lsp1109I GCAGC 7 cut(s) 176, 208, 400, 530, 556, 731, 1016
LweI GCATC 2 cut(s) 394, 951
MaeI CTAG 3 cut(s) 419, 636, 1013
MaeII ACGT 1 cut(s) 689
MalI GATC 2 cut(s) 283, 336
MbiI CCGCTC 1 cut(s) 874
MboI GATC 2 cut(s) 281, 334
MboII GAAGA 2 cut(s) 509, 654
MhlI GDGCHC 2 cut(s) 42, 712
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 4 cut(s) 265, 459, 972, 1021
MluNI TGGCCA 1 cut(s) 263
MmeI TCCRAC 2 cut(s) 165, 979
Mox20I TGGCCA 1 cut(s) 263
Mph1103I ATGCAT 1 cut(s) 944
MreI CGCCGGCG 1 cut(s) 59
MroNI GCCGGC 1 cut(s) 59
MroXI GAANNNNTTC 1 cut(s) 862
MscI TGGCCA 1 cut(s) 263
MseI TTAA 1 cut(s) 581
MslI CAYNNNNRTG 2 cut(s) 119, 450
Msp20I TGGCCA 1 cut(s) 263
MspA1I CMGCKG 2 cut(s) 221, 587
MspI CCGG 5 cut(s) 50, 60, 158, 315, 522
MspR9I CCNGG 3 cut(s) 158, 797, 960
MvaI CCWGG 2 cut(s) 797, 960
NaeI GCCGGC 1 cut(s) 61
NciI CCSGG 1 cut(s) 158
NdeII GATC 2 cut(s) 281, 334
NgoMIV GCCGGC 1 cut(s) 59
NheI GCTAGC 1 cut(s) 1012
NlaIII CATG 7 cut(s) 124, 383, 449, 708, 883, 1020, 1033
NlaIV GGNNCC 3 cut(s) 97, 162, 475
NsiI ATGCAT 1 cut(s) 944
NspI RCATGY 1 cut(s) 124
NspV TTCGAA 1 cut(s) 275
PaeI GCATGC 1 cut(s) 124
PaeR7I CTCGAG 1 cut(s) 174
PaqCI CACCTGC 1 cut(s) 232
PasI CCCWGGG 1 cut(s) 959
PceI AGGCCT 1 cut(s) 979
PdiI GCCGGC 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 862
PfeI GAWTC 4 cut(s) 778, 788, 863, 1037
Pfl23II CGTACG 1 cut(s) 483
PfoI TCCNGGA 1 cut(s) 795
PkrI GCNGC 9 cut(s) 58, 191, 220, 223, 415, 520, 571, 721, 1006
Ple19I CGATCG 1 cut(s) 284
PpuMI RGGWCCY 1 cut(s) 530
Psp5II RGGWCCY 1 cut(s) 530
Psp6I CCWGG 2 cut(s) 795, 958
PspFI CCCAGC 1 cut(s) 374
PspGI CCWGG 2 cut(s) 795, 958
PspLI CGTACG 1 cut(s) 483
PspN4I GGNNCC 3 cut(s) 97, 162, 475
PspPI GGNCC 5 cut(s) 474, 530, 597, 614, 848
PspPPI RGGWCCY 1 cut(s) 530
PspXI VCTCGAGB 1 cut(s) 174
PstI CTGCAG 1 cut(s) 997
PstNI CAGNNNCTG 1 cut(s) 755
PvuI CGATCG 1 cut(s) 284
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
RseI CAYNNNNRTG 2 cut(s) 119, 450
SaqAI TTAA 1 cut(s) 581
SatI GCNGC 9 cut(s) 57, 190, 219, 222, 414, 519, 570, 720, 1005
Sau3AI GATC 2 cut(s) 281, 334
Sau96I GGNCC 5 cut(s) 474, 530, 597, 614, 848
ScrFI CCNGG 3 cut(s) 158, 797, 960
SduI GDGCHC 2 cut(s) 42, 712
SfaNI GCATC 2 cut(s) 394, 951
SfcI CTRYAG 2 cut(s) 10, 993
Sfr274I CTCGAG 1 cut(s) 174
SfuI TTCGAA 1 cut(s) 275
SgrAI CRCCGGYG 1 cut(s) 59
SinI GGWCC 4 cut(s) 474, 530, 614, 848
SlaI CTCGAG 1 cut(s) 174
SmiMI CAYNNNNRTG 2 cut(s) 119, 450
SmlI CTYRAG 1 cut(s) 174
SmoI CTYRAG 1 cut(s) 174
SphI GCATGC 1 cut(s) 124
Sse9I AATT 4 cut(s) 265, 459, 972, 1021
SseBI AGGCCT 1 cut(s) 979
SsiI CCGC 7 cut(s) 57, 69, 93, 219, 251, 585, 872
SspMI CTAG 3 cut(s) 419, 636, 1013
StuI AGGCCT 1 cut(s) 979
StyD4I CCNGG 3 cut(s) 156, 795, 958
StyI CCWWGG 1 cut(s) 948
TaaI ACNGT 3 cut(s) 109, 503, 563
TaiI ACGT 1 cut(s) 692
TaqI TCGA 3 cut(s) 36, 175, 275
TasI AATT 4 cut(s) 265, 459, 972, 1021
TauI GCSGC 2 cut(s) 59, 221
TfiI GAWTC 4 cut(s) 778, 788, 863, 1037
Tru1I TTAA 1 cut(s) 581
Tru9I TTAA 1 cut(s) 581
TscAI CASTG 1 cut(s) 337
TseI GCWGC 7 cut(s) 189, 221, 413, 518, 569, 719, 1004
TspDTI ATGAA 5 cut(s) 17, 434, 687, 777, 1046
TspGWI ACGGA 2 cut(s) 33, 941
TspRI CASTG 1 cut(s) 337
VpaK11BI GGWCC 4 cut(s) 474, 530, 614, 848
XagI CCTNNNNNAGG 2 cut(s) 537, 1057
XapI RAATTY 1 cut(s) 1021
XceI RCATGY 1 cut(s) 124
XcmI CCANNNNNNNNNTGG 1 cut(s) 206
XhoI CTCGAG 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 862
XspI CTAG 3 cut(s) 419, 636, 1013
Zsp2I ATGCAT 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.