MD17G1177200.v1.1

At1g51745-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005767
Physical Location & Seq
Forward (+)
20131513 .. 20136724
5212 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1177200.v1.1.491

Sequence Viewer

Length: 2217 bp
ATGGGGAGTTCTGGTGAGACCAGTGAAAAGAGCATTGACGCGTCGGTGGGTAGTTTGGTTTGGGTCCGCCGCAGAAACGGGTCCTGGTGGCCGGGTCGGATAATGGGTCTGGACGAGTTGCCAGACAGTTGTGTGGTTTCGCCGAAATCGGGCACTCCGGTGAAGCTTCTCGGCCGTGATGACGCAAGCGTGGACTGGTATAACCTTGAAAGATCCAAAAGGGTGAAAGCTTTTCGTTGCGGGGAGTATGATGAATGCATTGAAAAAGCAAAGGCCCATGCAGCCAGCGGGAATAAGAAAAAAGTTAAATATGCACGGAGGGAAGATGCTATTATCCATGCTCTTGAGATTGAGAACGCTCGCCTAGGCAATGATGATTTGAACTTCAGCGCTCGAATGGGTAATTCAGGTGGTGACCATGGTTGCTCAGCCAGAGAATTACCTGCCATGTCTCATTCCAGCGAGGAGAATGAAGATATGGTTGACGATGTGAGTGATTCTGAAGACAATCCAGACTCAGGGCCAGAATTATCTCAATCTGGTATATCTTTTGAAGAGCCAAATCATATCAGTTCTTCTAAGTTGCAAACTCTGCAGGGAAGGAGAAGAAGAACACCAAATGATTCAGAGGATGATGGAACAGAAGGAGTTAAGCGCATGAGAGGACTTGAGGACCTCGGAATGGGTGTATTGTCAAAAAGGTCAGTCCAGACTGGAGGGCTTCTTGAACAAGTTCAACAGGATGGTGCTTCACTCTTGGATTCGAATGCTCGGAATGGAATGCCTAATGGAAGTCCTGTAAATGGAAGCAAAGGTACTTCATCACTGAAAAGAAAGAGATCTCAAGTGGCAAATGTTAATGAACTCTCAAAAAGAAAGAACCGCAGTCGACCATTGACAAAGGTGTTGGAGAGTACTGCAATGGTGTCTATTCCAGCTATGCCTGACCAACTTCCAAGTTCATGTGATTCACCTCTTCAAGGGTTATCTAATTGCAGGGTTTCCGGATTAGAATCCAATGAGTCAAAAGGGAGTTTATCTGCGGACCACACTGGAATTTCGTGTGAGAATGGAAATGCTCCTGAACTAGCTTCTGGTACTTCCTTTATCAATGACAAATTGAAAGACAGTGAGATTCCCAGCATATCTGGGATAGCGGAGAATGATTCTTCTGATGATAAGCTATTTGATGTGCCATTTGTTGGAGAGGAAAAAGACCCTTCAGGTTCTTCTCCTATACTTACAGCTTGTTCATCTGGTAAGCCTCAAAATGGTGCAATGGGAAGGCAATCTAGTCAAAGTCGTCAAGCTGAATCTGTAACTTTGAAAAATGAGAGAATTAACGAATCTGGTTGTACGAGTTCAGCTGCCTTGCATGATATCGGCATAAGTATAGATAAAGGTAGTTCAAAGTGGCAGTTAAAAGGAAAGAGGAATTCAAGACAAACAAGTAAAGATAGAACACATGACTCCAGAAGATATATGGACATGGATGATGGATCCAATACTTATTCGGCAGGCATCAAGCATTCCAATGGATTGTCTCCAGGTTCTTATCAGAAAGTCAATTGCAATGGCATTAGCGGTTCTGGTGCATATAATGGCACATTACGACCAAAGTCCAAACATGTTACTGAATTGCCTGATGGTCCAACTCAGAGATCACTTCCCTATCGGCAGTCTCGCTTCACAGTGCCTGCAAGATATCAGACGGCAGATTCTACCGCCCGCAATTCATGCTCTGATGGTTCATTGTATGATGTCGACCTTGAAGTGAAATCCAATTACCGGCCACAACATGTTCCGCTGGTTTCCCTGATGAGTAAATTGAACGGCAAAGCCATTGTTGGTCACCCACTAACAGTTGAGGTTTTGGGTGATGGCAGTTCTGATTGGGAAGTTGGTAAAACAGGCTATGCAATAAAGCCAAAAAAAGAAATTGGAAGAATCCCAGCCAAGGGATTGGCAGTGCAGCAGCGAAATTCACCAAGTAGGTCTCCCAAAACAAAGAAATCTGGGCTACAGCCAAAAAAGATCAGGAAACTATCTTCACTGACTGGTCACAAGCAATCTGAAGAGAGAAAACCACTGGTTCCGAAGCCAAAGGGTCCTGTAATAGCCTGTATCCCCCTTAAGGTAGTGTTCAGTAGGATAAATGAAGCAGTGAATGGGTTGGCGCGACCATCACATCGTGTTCTAAGATCAAGCAACGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

739

Amino Acids

79.6

Weight (kDa)

9.13

Isoelectric Point (pI)

58.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 17 - 105 2.3e-10 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 451
AccB7I CCANNNNNTGG 1 cut(s) 1202
AccI GTMKAC 2 cut(s) 889, 1764
AccII CGCG 2 cut(s) 41, 2179
AccIII TCCGGA 1 cut(s) 1004
AclWI GGATC 3 cut(s) 207, 1494, 1507
AcoI YGGCCR 3 cut(s) 89, 172, 1790
AcsI RAATTY 3 cut(s) 1056, 1435, 1981
AcuI CTGAAG 4 cut(s) 370, 522, 1206, 2094
AdeI CACNNNGTG 1 cut(s) 2192
AfaI GTAC 4 cut(s) 817, 916, 1099, 1357
AfeI AGCGCT 1 cut(s) 391
AflII CTTAAG 1 cut(s) 2132
AflIII ACRYGT 3 cut(s) 39, 1627, 1798
AjnI CCWGG 2 cut(s) 83, 1546
AleI CACNNNNGTG 1 cut(s) 158
AluBI AGCT 8 cut(s) 166, 230, 938, 1091, 1183, 1247, 1310, 1367
AluI AGCT 8 cut(s) 166, 230, 938, 1091, 1183, 1247, 1310, 1367
Alw26I GTCTC 5 cut(s) 11, 456, 1548, 1686, 2001
AlwI GGATC 3 cut(s) 207, 1494, 1507
AlwNI CAGNNNCTG 1 cut(s) 1697
Aor13HI TCCGGA 1 cut(s) 1004
Aor51HI AGCGCT 1 cut(s) 391
AoxI GGCC 5 cut(s) 89, 172, 273, 521, 1790
ApeKI GCWGC 4 cut(s) 281, 1367, 1972, 1975
ApoI RAATTY 3 cut(s) 1056, 1435, 1981
Asp700I GAANNNNTTC 1 cut(s) 732
AspA2I CCTAGG 1 cut(s) 364
AspLEI GCGC 3 cut(s) 392, 657, 2179
AspS9I GGNCC 8 cut(s) 64, 81, 274, 521, 673, 1045, 1649, 2108
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 8 cut(s) 26, 172, 235, 425, 963, 1844, 1889, 1977
AsuII TTCGAA 1 cut(s) 764
AvaII GGWCC 6 cut(s) 64, 81, 673, 1045, 1649, 2108
AvrII CCTAGG 1 cut(s) 364
BaeGI GKGCMC 1 cut(s) 155
BamHI GGATCC 1 cut(s) 1499
BarI GAAGNNNNNNTAC 2 cut(s) 799, 831
BbsI GAAGAC 1 cut(s) 510
BbvI GCAGC 4 cut(s) 293, 1354, 1984, 1987
BccI CCATC 7 cut(s) 629, 737, 1490, 1640, 1739, 1874, 2191
BceAI ACGGC 3 cut(s) 159, 1728, 1849
BciT130I CCWGG 2 cut(s) 85, 1548
BciVI GTATCC 1 cut(s) 2135
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 5 cut(s) 11, 456, 1548, 1686, 2001
BfaI CTAG 3 cut(s) 365, 1088, 1293
BfmI CTRYAG 2 cut(s) 593, 2021
BfoI RGCGCY 1 cut(s) 393
BfrI CTTAAG 1 cut(s) 2132
BfuAI ACCTGC 1 cut(s) 451
BfuI GTATCC 1 cut(s) 2135
BglII AGATCT 1 cut(s) 839
BisI GCNGC 5 cut(s) 70, 282, 1368, 1973, 1976
BlnI CCTAGG 1 cut(s) 364
BlpI GCTNAGC 1 cut(s) 427
BlsI GCNGC 5 cut(s) 71, 283, 1369, 1974, 1977
BmcAI AGTACT 1 cut(s) 916
Bme1390I CCNGG 3 cut(s) 85, 93, 1548
Bme18I GGWCC 6 cut(s) 64, 81, 673, 1045, 1649, 2108
BmgT120I GGNCC 8 cut(s) 64, 81, 274, 521, 673, 1045, 1649, 2108
BmiI GGNNCC 5 cut(s) 65, 82, 1501, 2094, 2109
BmrFI CCNGG 3 cut(s) 85, 93, 1548
BmsI GCATC 2 cut(s) 316, 1530
BoxI GACNNNNGTC 1 cut(s) 1618
BpiI GAAGAC 1 cut(s) 510
BpmI CTGGAG 3 cut(s) 735, 1456, 1530
Bpu1102I GCTNAGC 1 cut(s) 427
Bpu14I TTCGAA 1 cut(s) 764
BpuEI CTTGAG 3 cut(s) 365, 689, 828
BpuMI CCSGG 1 cut(s) 93
BsaBI GATNNNNATC 1 cut(s) 1012
BsaI GGTCTC 2 cut(s) 11, 2001
BsaJI CCNNGG 4 cut(s) 364, 418, 676, 1956
BsaWI WCCGGW 2 cut(s) 157, 1004
BsaXI ACNNNNNCTCC 1 cut(s) 28
Bse118I RCCGGY 1 cut(s) 1788
Bse1I ACTGG 6 cut(s) 21, 200, 718, 1057, 2062, 2094
Bse3DI GCAATG 4 cut(s) 376, 927, 1284, 1579
Bse8I GATNNNNATC 1 cut(s) 1012
BseAI TCCGGA 1 cut(s) 1004
BseBI CCWGG 2 cut(s) 85, 1548
BseDI CCNNGG 4 cut(s) 364, 418, 676, 1956
BseGI GGATG 3 cut(s) 637, 748, 1498
BseJI GATNNNNATC 1 cut(s) 1012
BseMI GCAATG 4 cut(s) 376, 927, 1284, 1579
BseMII CTCAG 3 cut(s) 441, 531, 1670
BseNI ACTGG 6 cut(s) 21, 200, 718, 1057, 2062, 2094
BseRI GAGGAG 1 cut(s) 479
BseSI GKGCMC 1 cut(s) 155
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 4 cut(s) 293, 1354, 1984, 1987
BseYI CCCAGC 2 cut(s) 1139, 1951
BsgI GTGCAG 1 cut(s) 1991
Bsh1236I CGCG 2 cut(s) 41, 2179
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 5 cut(s) 91, 174, 275, 523, 1792
BsiEI CGRYCG 1 cut(s) 175
BsiSI CCGG 4 cut(s) 92, 158, 1005, 1789
BsmAI GTCTC 5 cut(s) 11, 456, 1548, 1686, 2001
BsmI GAATGC 4 cut(s) 260, 772, 786, 1528
BsnI GGCC 5 cut(s) 91, 174, 275, 523, 1792
Bso31I GGTCTC 2 cut(s) 11, 2001
Bsp119I TTCGAA 1 cut(s) 764
Bsp1286I GDGCHC 1 cut(s) 155
Bsp13I TCCGGA 1 cut(s) 1004
Bsp143I GATC 6 cut(s) 212, 839, 1499, 1661, 2034, 2201
Bsp1720I GCTNAGC 1 cut(s) 427
Bsp19I CCATGG 1 cut(s) 418
BspANI GGCC 5 cut(s) 91, 174, 275, 523, 1792
BspCNI CTCAG 3 cut(s) 440, 530, 1669
BspEI TCCGGA 1 cut(s) 1004
BspFNI CGCG 2 cut(s) 41, 2179
BspLI GGNNCC 5 cut(s) 65, 82, 1501, 2094, 2109
BspMAI CTGCAG 1 cut(s) 597
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 3 cut(s) 207, 1494, 1507
BspQI GCTCTTC 1 cut(s) 549
BspT104I TTCGAA 1 cut(s) 764
BspTI CTTAAG 1 cut(s) 2132
BspTNI GGTCTC 2 cut(s) 11, 2001
BsrDI GCAATG 4 cut(s) 376, 927, 1284, 1579
BsrFI RCCGGY 1 cut(s) 1788
BsrI ACTGG 6 cut(s) 21, 200, 718, 1057, 2062, 2094
BssAI RCCGGY 1 cut(s) 1788
BssECI CCNNGG 4 cut(s) 364, 418, 676, 1956
BssMI GATC 6 cut(s) 212, 839, 1499, 1661, 2034, 2201
BssT1I CCWWGG 3 cut(s) 364, 418, 1956
Bst2UI CCWGG 2 cut(s) 85, 1548
Bst4CI ACNGT 4 cut(s) 128, 1130, 1693, 1864
Bst6I CTCTTC 3 cut(s) 549, 981, 2070
BstAFI CTTAAG 1 cut(s) 2132
BstAPI GCANNNNNTGC 2 cut(s) 592, 1737
BstBI TTCGAA 1 cut(s) 764
BstC8I GCNNGC 6 cut(s) 187, 286, 361, 1519, 1698, 1729
BstDEI CTNAG 5 cut(s) 427, 517, 579, 1656, 2198
BstDSI CCRYGG 1 cut(s) 418
BstEII GGTNACC 2 cut(s) 413, 1850
BstENI CCTNNNNNAGG 1 cut(s) 978
BstF5I GGATG 3 cut(s) 637, 748, 1498
BstFNI CGCG 2 cut(s) 41, 2179
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 3 cut(s) 392, 657, 2179
BstKTI GATC 6 cut(s) 215, 842, 1502, 1664, 2037, 2204
BstMAI GTCTC 5 cut(s) 11, 456, 1548, 1686, 2001
BstMBI GATC 6 cut(s) 212, 839, 1499, 1661, 2034, 2201
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 3 cut(s) 281, 592, 1737
BstNI CCWGG 2 cut(s) 85, 1548
BstNSI RCATGY 2 cut(s) 1631, 1802
BstPAI GACNNNNGTC 1 cut(s) 1618
BstPI GGTNACC 2 cut(s) 413, 1850
BstSCI CCNGG 3 cut(s) 83, 91, 1546
BstSFI CTRYAG 2 cut(s) 593, 2021
BstSLI GKGCMC 1 cut(s) 155
BstUI CGCG 2 cut(s) 41, 2179
BstV1I GCAGC 4 cut(s) 293, 1354, 1984, 1987
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 3 cut(s) 212, 839, 1499
BstXI CCANNNNNNTGG 1 cut(s) 1963
BstYI RGATCY 3 cut(s) 212, 839, 1499
BstZI CGGCCG 1 cut(s) 172
BsuI GTATCC 1 cut(s) 2135
BsuRI GGCC 5 cut(s) 91, 174, 275, 523, 1792
BtgI CCRYGG 1 cut(s) 418
BtsCI GGATG 3 cut(s) 637, 748, 1498
BtsI GCAGTG 2 cut(s) 1974, 2169
BtsIMutI CAGTG 9 cut(s) 28, 824, 1050, 1135, 1698, 1974, 2051, 2087, 2169
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 6 cut(s) 187, 286, 361, 1519, 1698, 1729
CaiI CAGNNNCTG 1 cut(s) 1697
CfoI GCGC 3 cut(s) 392, 657, 2179
Cfr10I RCCGGY 1 cut(s) 1788
Cfr13I GGNCC 8 cut(s) 64, 81, 274, 521, 673, 1045, 1649, 2108
CseI GACGC 3 cut(s) 30, 47, 191
Csp6I GTAC 4 cut(s) 816, 915, 1098, 1356
CviQI GTAC 4 cut(s) 816, 915, 1098, 1356
DdeI CTNAG 5 cut(s) 427, 517, 579, 1656, 2198
DpnI GATC 6 cut(s) 214, 841, 1501, 1663, 2036, 2203
DpnII GATC 6 cut(s) 212, 839, 1499, 1661, 2034, 2201
DraIII CACNNNGTG 1 cut(s) 2192
EaeI YGGCCR 3 cut(s) 89, 172, 1790
EagI CGGCCG 1 cut(s) 172
Eam1104I CTCTTC 3 cut(s) 549, 981, 2070
EarI CTCTTC 3 cut(s) 549, 981, 2070
EciI GGCGGA 1 cut(s) 56
EclXI CGGCCG 1 cut(s) 172
Eco130I CCWWGG 3 cut(s) 364, 418, 1956
Eco31I GGTCTC 2 cut(s) 11, 2001
Eco32I GATATC 2 cut(s) 1381, 1706
Eco47I GGWCC 6 cut(s) 64, 81, 673, 1045, 1649, 2108
Eco47III AGCGCT 1 cut(s) 391
Eco52I CGGCCG 1 cut(s) 172
Eco57I CTGAAG 4 cut(s) 370, 522, 1206, 2094
Eco91I GGTNACC 2 cut(s) 413, 1850
EcoNI CCTNNNNNAGG 1 cut(s) 978
EcoO109I RGGNCCY 3 cut(s) 81, 673, 2108
EcoO65I GGTNACC 2 cut(s) 413, 1850
EcoRI GAATTC 1 cut(s) 1435
EcoRII CCWGG 2 cut(s) 83, 1546
EcoRV GATATC 2 cut(s) 1381, 1706
EcoT14I CCWWGG 3 cut(s) 364, 418, 1956
EcoT22I ATGCAT 1 cut(s) 260
ErhI CCWWGG 3 cut(s) 364, 418, 1956
FalI AAGNNNNNCTT 1 cut(s) 2197
FauI CCCGC 3 cut(s) 233, 281, 1736
FblI GTMKAC 2 cut(s) 889, 1764
Fnu4HI GCNGC 5 cut(s) 70, 282, 1368, 1973, 1976
FokI GGATG 3 cut(s) 644, 755, 1505
Fsp4HI GCNGC 5 cut(s) 70, 282, 1368, 1973, 1976
FspBI CTAG 3 cut(s) 365, 1088, 1293
GlaI GCGC 3 cut(s) 391, 656, 2178
GluI GCNGC 5 cut(s) 70, 282, 1368, 1973, 1976
GsaI CCCAGC 2 cut(s) 1143, 1955
GsuI CTGGAG 3 cut(s) 735, 1456, 1530
HaeII RGCGCY 1 cut(s) 393
HaeIII GGCC 5 cut(s) 91, 174, 275, 523, 1792
HapII CCGG 4 cut(s) 92, 158, 1005, 1789
HgaI GACGC 3 cut(s) 30, 47, 191
HhaI GCGC 3 cut(s) 392, 657, 2179
Hin6I GCGC 3 cut(s) 390, 655, 2177
HinP1I GCGC 3 cut(s) 390, 655, 2177
HincII GTYRAC 3 cut(s) 484, 890, 1765
HindII GTYRAC 3 cut(s) 484, 890, 1765
HindIII AAGCTT 2 cut(s) 164, 228
HpaII CCGG 4 cut(s) 92, 158, 1005, 1789
HphI GGTGA 8 cut(s) 26, 172, 235, 425, 963, 1844, 1889, 1977
Hpy166II GTNNAC 4 cut(s) 193, 484, 890, 1765
Hpy8I GTNNAC 4 cut(s) 193, 484, 890, 1765
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 4 cut(s) 594, 638, 1230, 1278
HpyCH4III ACNGT 4 cut(s) 128, 1130, 1693, 1864
HpyF10VI GCNNNNNNNGC 3 cut(s) 281, 592, 1737
HpyF3I CTNAG 5 cut(s) 427, 517, 579, 1656, 2198
HspAI GCGC 3 cut(s) 390, 655, 2177
Kpn2I TCCGGA 1 cut(s) 1004
Kzo9I GATC 6 cut(s) 212, 839, 1499, 1661, 2034, 2201
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 1 cut(s) 1084
Lsp1109I GCAGC 4 cut(s) 293, 1354, 1984, 1987
LweI GCATC 2 cut(s) 316, 1530
MaeI CTAG 3 cut(s) 365, 1088, 1293
MaeIII GTNAC 5 cut(s) 413, 1318, 1630, 1850, 2060
MalI GATC 6 cut(s) 214, 841, 1501, 1663, 2036, 2203
MboI GATC 6 cut(s) 212, 839, 1499, 1661, 2034, 2201
MfeI CAATTG 1 cut(s) 1567
MflI RGATCY 3 cut(s) 212, 839, 1499
MhlI GDGCHC 1 cut(s) 155
MluI ACGCGT 1 cut(s) 39
MlyI GAGTC 3 cut(s) 509, 1031, 1463
MmeI TCCRAC 4 cut(s) 77, 888, 1183, 1676
Mph1103I ATGCAT 1 cut(s) 260
MroI TCCGGA 1 cut(s) 1004
MroXI GAANNNNTTC 1 cut(s) 732
MseI TTAA 6 cut(s) 306, 651, 858, 1341, 1421, 2133
MslI CAYNNNNRTG 2 cut(s) 158, 1533
MspA1I CMGCKG 3 cut(s) 288, 1367, 1807
MspCI CTTAAG 1 cut(s) 2132
MspI CCGG 4 cut(s) 92, 158, 1005, 1789
MspR9I CCNGG 3 cut(s) 85, 93, 1548
MunI CAATTG 1 cut(s) 1567
Mva1269I GAATGC 4 cut(s) 260, 772, 786, 1528
MvaI CCWGG 2 cut(s) 85, 1548
MvnI CGCG 2 cut(s) 41, 2179
MwoI GCNNNNNNNGC 3 cut(s) 281, 592, 1737
NciI CCSGG 1 cut(s) 93
NcoI CCATGG 1 cut(s) 418
NdeII GATC 6 cut(s) 212, 839, 1499, 1661, 2034, 2201
NlaIV GGNNCC 5 cut(s) 65, 82, 1501, 2094, 2109
NmeAIII GCCGAG 1 cut(s) 150
NmuCI GTSAC 3 cut(s) 413, 1850, 2060
NsiI ATGCAT 1 cut(s) 260
NspI RCATGY 2 cut(s) 1631, 1802
NspV TTCGAA 1 cut(s) 764
OliI CACNNNNGTG 1 cut(s) 158
PciI ACATGT 2 cut(s) 1627, 1798
PciSI GCTCTTC 1 cut(s) 549
PctI GAATGC 4 cut(s) 260, 772, 786, 1528
PdmI GAANNNNTTC 1 cut(s) 732
PflMI CCANNNNNTGG 1 cut(s) 1202
PkrI GCNGC 5 cut(s) 71, 283, 1369, 1974, 1977
PleI GAGTC 3 cut(s) 509, 1030, 1463
PpsI GAGTC 3 cut(s) 509, 1030, 1463
PpuMI RGGWCCY 3 cut(s) 81, 673, 2108
PscI ACATGT 2 cut(s) 1627, 1798
PshAI GACNNNNGTC 1 cut(s) 1618
Psp5II RGGWCCY 3 cut(s) 81, 673, 2108
Psp6I CCWGG 2 cut(s) 83, 1546
PspEI GGTNACC 2 cut(s) 413, 1850
PspFI CCCAGC 2 cut(s) 1139, 1951
PspGI CCWGG 2 cut(s) 83, 1546
PspN4I GGNNCC 5 cut(s) 65, 82, 1501, 2094, 2109
PspPI GGNCC 8 cut(s) 64, 81, 274, 521, 673, 1045, 1649, 2108
PspPPI RGGWCCY 3 cut(s) 81, 673, 2108
PstI CTGCAG 1 cut(s) 597
PstNI CAGNNNCTG 1 cut(s) 1697
PsuI RGATCY 3 cut(s) 212, 839, 1499
PvuII CAGCTG 1 cut(s) 1367
RsaI GTAC 4 cut(s) 817, 916, 1099, 1357
RsaNI GTAC 4 cut(s) 816, 915, 1098, 1356
RseI CAYNNNNRTG 2 cut(s) 158, 1533
SalI GTCGAC 2 cut(s) 888, 1763
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 6 cut(s) 306, 651, 858, 1341, 1421, 2133
SatI GCNGC 5 cut(s) 70, 282, 1368, 1973, 1976
Sau3AI GATC 6 cut(s) 212, 839, 1499, 1661, 2034, 2201
Sau96I GGNCC 8 cut(s) 64, 81, 274, 521, 673, 1045, 1649, 2108
ScaI AGTACT 1 cut(s) 916
SchI GAGTC 3 cut(s) 509, 1031, 1463
ScrFI CCNGG 3 cut(s) 85, 93, 1548
SduI GDGCHC 1 cut(s) 155
SfaNI GCATC 2 cut(s) 316, 1530
SfcI CTRYAG 2 cut(s) 593, 2021
SfuI TTCGAA 1 cut(s) 764
SinI GGWCC 6 cut(s) 64, 81, 673, 1045, 1649, 2108
SmiMI CAYNNNNRTG 2 cut(s) 158, 1533
SmlI CTYRAG 4 cut(s) 344, 668, 843, 2132
SmoI CTYRAG 4 cut(s) 344, 668, 843, 2132
SspMI CTAG 3 cut(s) 365, 1088, 1293
StyD4I CCNGG 3 cut(s) 83, 91, 1546
StyI CCWWGG 3 cut(s) 364, 418, 1956
TaaI ACNGT 4 cut(s) 128, 1130, 1693, 1864
TaqI TCGA 4 cut(s) 394, 764, 889, 1764
TatI WGTACW 1 cut(s) 914
TauI GCSGC 1 cut(s) 72
Tru1I TTAA 6 cut(s) 306, 651, 858, 1341, 1421, 2133
Tru9I TTAA 6 cut(s) 306, 651, 858, 1341, 1421, 2133
TscAI CASTG 9 cut(s) 28, 831, 1057, 1135, 1698, 1974, 2058, 2094, 2169
TseFI GTSAC 3 cut(s) 413, 1850, 2060
TseI GCWGC 4 cut(s) 281, 1367, 1972, 1975
Tsp45I GTSAC 3 cut(s) 413, 1850, 2060
TspDTI ATGAA 9 cut(s) 267, 486, 810, 876, 951, 1242, 1725, 1740, 2172
TspGWI ACGGA 1 cut(s) 331
TspRI CASTG 9 cut(s) 28, 831, 1057, 1135, 1698, 1974, 2058, 2094, 2169
Van91I CCANNNNNTGG 1 cut(s) 1202
Vha464I CTTAAG 1 cut(s) 2132
VpaK11BI GGWCC 6 cut(s) 64, 81, 673, 1045, 1649, 2108
XagI CCTNNNNNAGG 1 cut(s) 978
XapI RAATTY 3 cut(s) 1056, 1435, 1981
XceI RCATGY 2 cut(s) 1631, 1802
XcmI CCANNNNNNNNNTGG 1 cut(s) 1480
XmaJI CCTAGG 1 cut(s) 364
XmiI GTMKAC 2 cut(s) 889, 1764
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 3 cut(s) 365, 1088, 1293
ZrmI AGTACT 1 cut(s) 916
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.