Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0008150

Physical Location & Seq

Genomic Coordinates Reverse (-)

25513885 .. 25517507

Gene Structure 3623 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1210300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2451 bp

ATGGGTCGGTTTCAGATTAGAGCCTTGTGTTTCTGTGTCCTCCTACTTGTGTTCAAAACCTGCATAGCCGACGAACAACACAAAGATCGAATCTATCCCGGGTTCCAGGCATCTCAGGTGGACTGGAGTGATCATGTGGGGCTGTTCCTGCTATCGAACAACTCGGCGTTCGCCTTAGGGTTCTACCAAGCGCTGGATGTCAAGTTGTATTTACTTGTGATTATTCACTTGGGTACTGATAAAGTGGTCTGGACTGCTAACAGAGGCAAACTGATTTCCAAGTCCGCTAAGTTCGTTTTTGATAAAAATGGGAGTGCCTACTTGAGAGGCAGTGATAGTGTGGCCTGGTCTACAAACACAACAGGACAGGTAGCTTCAGCCATGGAATTGCAGGATTCAGGAAACTTGGTGTTGCTTGGAGACAAAGGAAGCATTCTTTGGCAGAGTTTTAGCCATCCCACGGACACTCTCTTACCTGGCCAGGAGTTCTTGCAAGGAATGCAGCTCAAAAGTTTTCGCAACTCCAATAACGTGTTTCACTATCTTGAAATTCAGTCAGGGGATCTGGTCTTGTATACAGGATACGAACCTCCTCAAATCTATTGGTCCTTGGCGGTTGACAGCAGGATGAGCAACAACAACGTCATTGGCAATATTCACTCTCTGTCTCTGGCGTCCAATGCTTTGAATTTCTATGATGCAAATAAATCCTTGCTTTGGCAATTTGTCTTCTCTGATAACAAGGACCCGAATGCATTCTGGTCTGCGGCTTTAGGAGCTGATGCAGTGATCTCCTTTTACGATCTTCAGAAGGGAAAGTCTGTTACTTCTGAAGCAACTAAGATCCCGCAAAATTCATGCAGCACTCCTGAGCCTTGTGCCCCTTATTTTGTGTGCTACTTTGACAATTGGTGCCAATGTCCTTCGCTTCTCACTTCCCGCTTTAATTGCAGACCTCAAGCACTCCCAACTTGTAATAGCTCCAGAAGCTCAACGGAGCTTTTATACGTTGGTGAGAAGCTCGATTACTTTGCCCTTCAGTTTACCAAACCCTCCCTGCTGAAATCCAATCTGAGTTCCTGCAAAGAAGCTTGCATTAGCAATTGTTCTTGCCTTGTGCTCTTCTTTGAAAGCAGCTCTGGACATTGTTATATGTTTGACCGGGTAGGGAGTTTTGAACGCTCCGGTGCAAATTCTACCGGGTACATCTCTTATATGAAGATTTTAAGTGATGAAGGAATCAATGCCGGACAAGGAAAGGGAAACAGAAGCAAACACATCTTGTTAATCATGGTCATTTTGATTGCAACAATTGTTGTTATTGCTGGTCTAGTTTATATGGGATTCTTGTACTACCGCAGGAAGAGACTACTTGATTATTCTCAAGAAATCTTGGAAGAGGATAAGTTCTTGGACAGTCTTTCTGGGATGCCTATTCGCTTCATGTACGGTGATCTTAGCAGAGCAACTAAAAATTTCTCCAACAAAATTGGCCAAGGAGGGTTTGGTTCAGTCTACGTAGGCGAGCTCCCAGACGGCATCCAACTGGCTGTGAAAAAACTGGAGGGCCTTGGCCAGGGGAAGAAAGAGTTTAGAGCTGAGGTCACTATTATTGGGAAAATTCGTCACGTCCATCTGGTCAAGCTCAGAGGTTTCTGTGCAGAAGGGCCTCACCGGCTTCTTGTTTACGAGTACATGGGAAAAGGGTCTTTAGATAAGTGGATATTCAATAGCAACAAAAAGGAGGCCGTCTTGGATTGGAACACAAGATTCAATATTGCACTGGGCACTGCAAAGGGGTTGGCGTATCTACATGAAGAATGTGAAGAGAAGATTGTCCACTGCGATATAAAACCTGAAAATGTTCTTCTTGATGACAATTTTGTTGCCAAAGTTTCAGATTTTGGTTTGGCAAAGATGATGAACCGGGAGGAAAACCTTGTACATACAACGGTGAGGGGTACAAGAGGGTACCTTGCTCCGGAATGGGTTACCAATCATGCCATATCAGAGAAGAGTGATGTGTACAGTTACGGCATGGTGTTGCTTGAGATCATTGGTGGTAAGAGGAATTTTGATCCAGTGAACAGTTCTGAGAGAGGCCACTTTCCTTCTTATGCATTTAAGCTGTTGGAAGAGGGAAATCTGAAACAATTCCTCGATCCGAAGCTAGATGTTGATGAAAATGATGAAAGGGTTGTGACTGCAATCAATGTTGCATTGTGGTGCATTCAAGATGAGATGCATATGAGGCCTCCGATGACGAAAGTAGTCCAAATGCTTGAAGATCTTTCTGCTTTACCACCACCACGGATTTCCTCCTCCTCTCTGTCAGATCGTCGTGTGAGTTTCTTACAATGGAGCGGCAAGGAAGCTAGTTCGTCGTCAGGACTCACTAATTACAACAGTGATAGACTCCTGTCGGACATTCAACTTTCGGGGCCAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_1 Pkinase

Protein Analysis

817

Amino Acids

91.32

Weight (kDa)

6.79

Isoelectric Point (pI)

36.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	82 - 164	2.2e-17	D-mannose binding lectin
PK_Tyr_Ser-Thr	PF07714	493 - 760	2.1e-42	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	494 - 759	2e-47	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008150)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g29821 FvH4_6g29840
malus_domestica	MD17G1210300.v1.1
prunus_persica	Prupe.3G085600_v2.0.a1
pyrus_communis	pycom17g21560
rosa_chinensis	RchiOBHm_Chr2g0135701
rosa_laevigata	RLG00000019478 RLG00000019479
rosa_multiflora	Rmu_co8459773.1_g000001 Rmu_sc0002398.1_g000047 Rmu_sc0007848.1_g000046
rosa_roxburghii	Rroxscaffold_2G00108980 Rroxscaffold_2G00109000
rosa_rugosa	Rorug02G0324900 Rorug02G0325100
rosa_samantha	Rh2AG377400 Rh2AG377500 Rh2BG383700 Rh2BG383800 Rh2CG362900 Rh2CG363000 Rh2DG400100
rosa_wichuraiana	Rw2G030740 Rw2G030750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	68
Acc65I	GGTACC	1 cut(s)	1971
AccB1I	GGYRCC	2 cut(s)	912, 1971
AccB7I	CCANNNNNTGG	1 cut(s)	193
AccBSI	CCGCTC	1 cut(s)	2364
AccI	GTMKAC	3 cut(s)	350, 575, 1515
AccIII	TCCGGA	1 cut(s)	1981
AciI	CCGC	7 cut(s)	285, 614, 767, 848, 940, 1357, 2364
AclWI	GGATC	4 cut(s)	570, 838, 2072, 2156
AcoI	YGGCCR	3 cut(s)	478, 1492, 1573
AcsI	RAATTY	7 cut(s)	549, 688, 853, 1192, 1474, 1620, 2071
AcuI	CTGAAG	4 cut(s)	360, 791, 852, 1022
AcyI	GRCGYC	1 cut(s)	674
AfaI	GTAC	9 cut(s)	235, 1205, 1352, 1448, 1694, 1944, 1963, 1973, 2027
AfeI	AGCGCT	1 cut(s)	192
AfiI	CCNNNNNNNGG	5 cut(s)	193, 460, 717, 1576, 1981
AflIII	ACRYGT	1 cut(s)	531
AjiI	CACGTC	1 cut(s)	1630
AjnI	CCWGG	5 cut(s)	105, 344, 475, 480, 1575
AjuI	GAANNNNNNNTTGG	2 cut(s)	421, 453
Alw21I	GWGCWC	2 cut(s)	1122, 1530
Alw26I	GTCTC	3 cut(s)	414, 672, 1360
AlwI	GGATC	4 cut(s)	570, 838, 2072, 2156
Ama87I	CYCGRG	1 cut(s)	98
Aor13HI	TCCGGA	1 cut(s)	1981
Aor51HI	AGCGCT	1 cut(s)	192
ApeKI	GCWGC	3 cut(s)	502, 861, 1134
ApoI	RAATTY	7 cut(s)	549, 688, 853, 1192, 1474, 1620, 2071
Asp700I	GAANNNNTTC	3 cut(s)	755, 1863, 2153
Asp718I	GGTACC	1 cut(s)	1971
AspLEI	GCGC	1 cut(s)	193
AspS9I	GGNCC	5 cut(s)	606, 745, 1567, 1667, 2441
AsuC2I	CCSGG	5 cut(s)	99, 100, 1163, 1201, 1928
AsuHPI	GGTGA	4 cut(s)	1025, 1463, 1664, 1966
AvaI	CYCGRG	1 cut(s)	98
AvaII	GGWCC	2 cut(s)	606, 745
AxyI	CCTNAGG	1 cut(s)	175
BaeGI	GKGCMC	2 cut(s)	883, 1790
BalI	TGGCCA	3 cut(s)	480, 1494, 1575
BanI	GGYRCC	2 cut(s)	912, 1971
BanII	GRGCYC	1 cut(s)	1530
BarI	GAAGNNNNNNTAC	2 cut(s)	1010, 1042
BbsI	GAAGAC	1 cut(s)	721
Bbv12I	GWGCWC	2 cut(s)	1122, 1530
BbvCI	CCTCAGC	1 cut(s)	1599
BbvI	GCAGC	3 cut(s)	514, 873, 1146
BccI	CCATC	2 cut(s)	462, 1641
BceAI	ACGGC	3 cut(s)	1552, 1733, 2050
BcgI	CGANNNNNNTGC	2 cut(s)	1013, 1047
BciT130I	CCWGG	5 cut(s)	107, 346, 477, 482, 1577
BciVI	GTATCC	1 cut(s)	575
BclI	TGATCA	1 cut(s)	130
BcnI	CCSGG	5 cut(s)	99, 100, 1163, 1201, 1928
BcoDI	GTCTC	3 cut(s)	414, 672, 1360
BfaI	CTAG	3 cut(s)	1331, 2171, 2376
BfoI	RGCGCY	1 cut(s)	194
BfuAI	ACCTGC	1 cut(s)	68
BfuI	GTATCC	1 cut(s)	575
BglI	GCCNNNNNGGC	1 cut(s)	1675
BglII	AGATCT	1 cut(s)	2287
BisI	GCNGC	5 cut(s)	503, 768, 862, 1135, 2365
BlsI	GCNGC	5 cut(s)	504, 769, 863, 1136, 2366
Bme18I	GGWCC	2 cut(s)	606, 745
BmeT110I	CYCGRG	1 cut(s)	98
BmgBI	CACGTC	1 cut(s)	1630
BmgT120I	GGNCC	5 cut(s)	606, 745, 1567, 1667, 2441
BmiI	GGNNCC	5 cut(s)	104, 747, 914, 1973, 2442
BmrI	ACTGGG	1 cut(s)	1793
BmsI	GCATC	6 cut(s)	119, 688, 772, 1419, 1548, 2232
BmuI	ACTGGG	1 cut(s)	1793
BoxI	GACNNNNGTC	1 cut(s)	2419
BpiI	GAAGAC	1 cut(s)	721
BpmI	CTGGAG	3 cut(s)	145, 967, 1583
Bpu10I	CCTNAGC	2 cut(s)	870, 1599
BpuEI	CTTGAG	4 cut(s)	343, 942, 1368, 2069
BpuMI	CCSGG	5 cut(s)	99, 100, 1163, 1201, 1928
BsaAI	YACGTR	1 cut(s)	1519
BsaHI	GRCGYC	1 cut(s)	674
BsaJI	CCNNGG	8 cut(s)	98, 381, 459, 609, 1495, 1570, 1576, 2309
BsaWI	WCCGGW	2 cut(s)	1184, 1981
Bsc4I	CCNNNNNNNGG	5 cut(s)	193, 460, 717, 1576, 1981
Bse118I	RCCGGY	1 cut(s)	1674
Bse1I	ACTGG	5 cut(s)	128, 1551, 1566, 1788, 2081
Bse21I	CCTNAGG	1 cut(s)	175
BseAI	TCCGGA	1 cut(s)	1981
BseBI	CCWGG	5 cut(s)	107, 346, 477, 482, 1577
BseDI	CCNNGG	8 cut(s)	98, 381, 459, 609, 1495, 1570, 1576, 2309
BseGI	GGATG	5 cut(s)	202, 454, 633, 1434, 1539
BseLI	CCNNNNNNNGG	5 cut(s)	193, 460, 717, 1576, 1981
BseMII	CTCAG	6 cut(s)	128, 861, 1064, 1590, 1660, 2085
BseNI	ACTGG	5 cut(s)	128, 1551, 1566, 1788, 2081
BseRI	GAGGAG	3 cut(s)	582, 2311, 2314
BseSI	GKGCMC	2 cut(s)	883, 1790
BseXI	GCAGC	3 cut(s)	514, 873, 1146
BsgI	GTGCAG	1 cut(s)	1680
BshNI	GGYRCC	2 cut(s)	912, 1971
BsiHKAI	GWGCWC	2 cut(s)	1122, 1530
BsiHKCI	CYCGRG	1 cut(s)	98
BsiSI	CCGG	8 cut(s)	99, 1162, 1185, 1200, 1248, 1675, 1927, 1982
BslI	CCNNNNNNNGG	5 cut(s)	193, 460, 717, 1576, 1981
BsmAI	GTCTC	3 cut(s)	414, 672, 1360
BsmI	GAATGC	5 cut(s)	432, 504, 755, 757, 2229
BsoBI	CYCGRG	1 cut(s)	98
Bsp1286I	GDGCHC	4 cut(s)	883, 1122, 1530, 1790
Bsp13I	TCCGGA	1 cut(s)	1981
Bsp1407I	TGTACA	2 cut(s)	1942, 2025
Bsp19I	CCATGG	1 cut(s)	381
BspACI	CCGC	7 cut(s)	285, 614, 767, 848, 940, 1357, 2364
BspCNI	CTCAG	6 cut(s)	127, 862, 1065, 1591, 1659, 2086
BspEI	TCCGGA	1 cut(s)	1981
BspLI	GGNNCC	5 cut(s)	104, 747, 914, 1973, 2442
BspMI	ACCTGC	1 cut(s)	68
BspPI	GGATC	4 cut(s)	570, 838, 2072, 2156
BspQI	GCTCTTC	1 cut(s)	1127
BspT107I	GGYRCC	2 cut(s)	912, 1971
BsrBI	CCGCTC	1 cut(s)	2364
BsrFI	RCCGGY	1 cut(s)	1674
BsrGI	TGTACA	2 cut(s)	1942, 2025
BsrI	ACTGG	5 cut(s)	128, 1551, 1566, 1788, 2081
BssAI	RCCGGY	1 cut(s)	1674
BssECI	CCNNGG	8 cut(s)	98, 381, 459, 609, 1495, 1570, 1576, 2309
BssNAI	GTATAC	1 cut(s)	576
BssNI	GRCGYC	1 cut(s)	674
BssT1I	CCWWGG	4 cut(s)	381, 609, 1495, 1570
Bst1107I	GTATAC	1 cut(s)	576
Bst2UI	CCWGG	5 cut(s)	107, 346, 477, 482, 1577
Bst4CI	ACNGT	6 cut(s)	1418, 1451, 1954, 2030, 2090, 2408
Bst6I	CTCTTC	6 cut(s)	1127, 1358, 1392, 1821, 2009, 2130
BstACI	GRCGYC	1 cut(s)	674
BstAPI	GCANNNNNTGC	1 cut(s)	499
BstAUI	TGTACA	2 cut(s)	1942, 2025
BstBAI	YACGTR	1 cut(s)	1519
BstC8I	GCNNGC	2 cut(s)	1093, 1526
BstDSI	CCRYGG	3 cut(s)	381, 459, 2309
BstEII	GGTNACC	1 cut(s)	1990
BstENI	CCTNNNNNAGG	1 cut(s)	1574
BstF5I	GGATG	5 cut(s)	202, 454, 633, 1434, 1539
BstH2I	RGCGCY	1 cut(s)	194
BstHHI	GCGC	1 cut(s)	193
BstMAI	GTCTC	3 cut(s)	414, 672, 1360
BstMWI	GCNNNNNNNGC	9 cut(s)	148, 499, 630, 680, 776, 948, 987, 1675, 2251
BstNI	CCWGG	5 cut(s)	107, 346, 477, 482, 1577
BstPAI	GACNNNNGTC	1 cut(s)	2419
BstPI	GGTNACC	1 cut(s)	1990
BstSLI	GKGCMC	2 cut(s)	883, 1790
BstSNI	TACGTA	1 cut(s)	1519
BstV1I	GCAGC	3 cut(s)	514, 873, 1146
BstV2I	GAAGAC	1 cut(s)	721
BstX2I	RGATCY	3 cut(s)	562, 843, 2287
BstYI	RGATCY	3 cut(s)	562, 843, 2287
BstZ17I	GTATAC	1 cut(s)	576
Bsu36I	CCTNAGG	1 cut(s)	175
BsuI	GTATCC	1 cut(s)	575
BtgI	CCRYGG	3 cut(s)	381, 459, 2309
BtrI	CACGTC	1 cut(s)	1630
BtsCI	GGATG	5 cut(s)	202, 454, 633, 1434, 1539
BtsI	GCAGTG	4 cut(s)	337, 792, 1788, 1840
BtsIMutI	CAGTG	7 cut(s)	337, 792, 1781, 1788, 1840, 2088, 2413
BveI	ACCTGC	1 cut(s)	68
Cac8I	GCNNGC	2 cut(s)	1093, 1526
CfoI	GCGC	1 cut(s)	193
Cfr10I	RCCGGY	1 cut(s)	1674
Cfr13I	GGNCC	5 cut(s)	606, 745, 1567, 1667, 2441
Cfr9I	CCCGGG	1 cut(s)	98
CseI	GACGC	1 cut(s)	663
Csp6I	GTAC	9 cut(s)	234, 1204, 1351, 1447, 1693, 1943, 1962, 1972, 2026
CviAII	CATG	9 cut(s)	134, 382, 858, 1291, 1444, 1696, 1814, 2000, 2038
CviQI	GTAC	9 cut(s)	234, 1204, 1351, 1447, 1693, 1943, 1962, 1972, 2026
EaeI	YGGCCR	3 cut(s)	478, 1492, 1573
Eam1104I	CTCTTC	6 cut(s)	1127, 1358, 1392, 1821, 2009, 2130
EarI	CTCTTC	6 cut(s)	1127, 1358, 1392, 1821, 2009, 2130
Ecl136II	GAGCTC	1 cut(s)	1528
Eco105I	TACGTA	1 cut(s)	1519
Eco130I	CCWWGG	4 cut(s)	381, 609, 1495, 1570
Eco147I	AGGCCT	1 cut(s)	2254
Eco24I	GRGCYC	1 cut(s)	1530
Eco47I	GGWCC	2 cut(s)	606, 745
Eco47III	AGCGCT	1 cut(s)	192
Eco53kI	GAGCTC	1 cut(s)	1528
Eco57I	CTGAAG	4 cut(s)	360, 791, 852, 1022
Eco81I	CCTNAGG	1 cut(s)	175
Eco88I	CYCGRG	1 cut(s)	98
Eco91I	GGTNACC	1 cut(s)	1990
EcoICRI	GAGCTC	1 cut(s)	1528
EcoNI	CCTNNNNNAGG	1 cut(s)	1574
EcoO109I	RGGNCCY	3 cut(s)	745, 1567, 1667
EcoO65I	GGTNACC	1 cut(s)	1990
EcoRII	CCWGG	5 cut(s)	105, 344, 475, 480, 1575
EcoT14I	CCWWGG	4 cut(s)	381, 609, 1495, 1570
EcoT22I	ATGCAT	3 cut(s)	757, 2122, 2247
EcoT38I	GRGCYC	1 cut(s)	1530
ErhI	CCWWGG	4 cut(s)	381, 609, 1495, 1570
FaeI	CATG	9 cut(s)	137, 385, 861, 1294, 1447, 1699, 1817, 2003, 2041
FatI	CATG	9 cut(s)	133, 381, 857, 1290, 1443, 1695, 1813, 1999, 2037
FauI	CCCGC	2 cut(s)	855, 947
FauNDI	CATATG	1 cut(s)	2247
FbaI	TGATCA	1 cut(s)	130
FblI	GTMKAC	3 cut(s)	350, 575, 1515
Fnu4HI	GCNGC	5 cut(s)	503, 768, 862, 1135, 2365
FokI	GGATG	5 cut(s)	209, 441, 640, 1441, 1526
FriOI	GRGCYC	1 cut(s)	1530
Fsp4HI	GCNGC	5 cut(s)	503, 768, 862, 1135, 2365
FspBI	CTAG	3 cut(s)	1331, 2171, 2376
GlaI	GCGC	1 cut(s)	192
GluI	GCNGC	5 cut(s)	503, 768, 862, 1135, 2365
GsuI	CTGGAG	3 cut(s)	145, 967, 1583
HaeII	RGCGCY	1 cut(s)	194
HapII	CCGG	8 cut(s)	99, 1162, 1185, 1200, 1248, 1675, 1927, 1982
HgaI	GACGC	1 cut(s)	663
HhaI	GCGC	1 cut(s)	193
Hin1I	GRCGYC	1 cut(s)	674
Hin1II	CATG	9 cut(s)	137, 385, 861, 1294, 1447, 1699, 1817, 2003, 2041
Hin6I	GCGC	1 cut(s)	191
HinP1I	GCGC	1 cut(s)	191
HincII	GTYRAC	1 cut(s)	619
HindII	GTYRAC	1 cut(s)	619
HindIII	AAGCTT	1 cut(s)	1089
HinfI	GANTC	7 cut(s)	90, 395, 1239, 1344, 1770, 2391, 2415
HpaII	CCGG	8 cut(s)	99, 1162, 1185, 1200, 1248, 1675, 1927, 1982
HphI	GGTGA	4 cut(s)	1025, 1463, 1664, 1966
Hpy99I	CGWCG	3 cut(s)	74, 2343, 2386
HpyAV	CCTTC	6 cut(s)	805, 933, 1046, 1229, 1658, 2121
HpyCH4III	ACNGT	6 cut(s)	1418, 1451, 1954, 2030, 2090, 2408
HpyCH4IV	ACGT	5 cut(s)	531, 642, 1008, 1518, 1629
HpyF10VI	GCNNNNNNNGC	9 cut(s)	148, 499, 630, 680, 776, 948, 987, 1675, 2251
HpySE526I	ACGT	5 cut(s)	531, 642, 1008, 1518, 1629
Hsp92I	GRCGYC	1 cut(s)	674
Hsp92II	CATG	9 cut(s)	137, 385, 861, 1294, 1447, 1699, 1817, 2003, 2041
HspAI	GCGC	1 cut(s)	191
Kpn2I	TCCGGA	1 cut(s)	1981
KpnI	GGTACC	1 cut(s)	1975
Ksp22I	TGATCA	1 cut(s)	130
LguI	GCTCTTC	1 cut(s)	1127
LmnI	GCTCC	7 cut(s)	776, 986, 997, 1187, 1533, 1984, 2361
Lsp1109I	GCAGC	3 cut(s)	514, 873, 1146
LweI	GCATC	6 cut(s)	119, 688, 772, 1419, 1548, 2232
MaeI	CTAG	3 cut(s)	1331, 2171, 2376
MaeII	ACGT	5 cut(s)	531, 642, 1008, 1518, 1629
MaeIII	GTNAC	6 cut(s)	823, 1603, 1625, 1990, 2030, 2200
MbiI	CCGCTC	1 cut(s)	2364
MfeI	CAATTG	3 cut(s)	907, 1102, 1311
MflI	RGATCY	3 cut(s)	562, 843, 2287
MhlI	GDGCHC	4 cut(s)	883, 1122, 1530, 1790
MlsI	TGGCCA	3 cut(s)	480, 1494, 1575
MluNI	TGGCCA	3 cut(s)	480, 1494, 1575
MlyI	GAGTC	2 cut(s)	2385, 2409
MmeI	TCCRAC	4 cut(s)	1506, 1567, 2112, 2403
Mox20I	TGGCCA	3 cut(s)	480, 1494, 1575
Mph1103I	ATGCAT	3 cut(s)	757, 2122, 2247
MroI	TCCGGA	1 cut(s)	1981
MroXI	GAANNNNTTC	3 cut(s)	755, 1863, 2153
MscI	TGGCCA	3 cut(s)	480, 1494, 1575
MseI	TTAA	4 cut(s)	945, 1226, 1286, 2124
MslI	CAYNNNNRTG	1 cut(s)	2224
Msp20I	TGGCCA	3 cut(s)	480, 1494, 1575
MspI	CCGG	8 cut(s)	99, 1162, 1185, 1200, 1248, 1675, 1927, 1982
MunI	CAATTG	3 cut(s)	907, 1102, 1311
Mva1269I	GAATGC	5 cut(s)	432, 504, 755, 757, 2229
MvaI	CCWGG	5 cut(s)	107, 346, 477, 482, 1577
MwoI	GCNNNNNNNGC	9 cut(s)	148, 499, 630, 680, 776, 948, 987, 1675, 2251
NciI	CCSGG	5 cut(s)	99, 100, 1163, 1201, 1928
NcoI	CCATGG	1 cut(s)	381
NdeI	CATATG	1 cut(s)	2247
NlaIII	CATG	9 cut(s)	137, 385, 861, 1294, 1447, 1699, 1817, 2003, 2041
NlaIV	GGNNCC	5 cut(s)	104, 747, 914, 1973, 2442
NmeAIII	GCCGAG	1 cut(s)	143
NmuCI	GTSAC	3 cut(s)	1603, 1625, 2200
NsiI	ATGCAT	3 cut(s)	757, 2122, 2247
PceI	AGGCCT	1 cut(s)	2254
PciSI	GCTCTTC	1 cut(s)	1127
PctI	GAATGC	5 cut(s)	432, 504, 755, 757, 2229
PdmI	GAANNNNTTC	3 cut(s)	755, 1863, 2153
PfeI	GAWTC	5 cut(s)	90, 395, 1239, 1344, 1770
PflMI	CCANNNNNTGG	1 cut(s)	193
PkrI	GCNGC	5 cut(s)	504, 769, 863, 1136, 2366
PleI	GAGTC	2 cut(s)	2385, 2409
PpsI	GAGTC	2 cut(s)	2385, 2409
Ppu21I	YACGTR	1 cut(s)	1519
PpuMI	RGGWCCY	1 cut(s)	745
PshAI	GACNNNNGTC	1 cut(s)	2419
Psp124BI	GAGCTC	1 cut(s)	1530
Psp5II	RGGWCCY	1 cut(s)	745
Psp6I	CCWGG	5 cut(s)	105, 344, 475, 480, 1575
PspEI	GGTNACC	1 cut(s)	1990
PspGI	CCWGG	5 cut(s)	105, 344, 475, 480, 1575
PspN4I	GGNNCC	5 cut(s)	104, 747, 914, 1973, 2442
PspPI	GGNCC	5 cut(s)	606, 745, 1567, 1667, 2441
PspPPI	RGGWCCY	1 cut(s)	745
PsuI	RGATCY	3 cut(s)	562, 843, 2287
RsaI	GTAC	9 cut(s)	235, 1205, 1352, 1448, 1694, 1944, 1963, 1973, 2027
RsaNI	GTAC	9 cut(s)	234, 1204, 1351, 1447, 1693, 1943, 1962, 1972, 2026
RseI	CAYNNNNRTG	1 cut(s)	2224
SacI	GAGCTC	1 cut(s)	1530
SapI	GCTCTTC	1 cut(s)	1127
SaqAI	TTAA	4 cut(s)	945, 1226, 1286, 2124
SatI	GCNGC	5 cut(s)	503, 768, 862, 1135, 2365
Sau96I	GGNCC	5 cut(s)	606, 745, 1567, 1667, 2441
SchI	GAGTC	2 cut(s)	2385, 2409
SduI	GDGCHC	4 cut(s)	883, 1122, 1530, 1790
SfaNI	GCATC	6 cut(s)	119, 688, 772, 1419, 1548, 2232
SinI	GGWCC	2 cut(s)	606, 745
SmaI	CCCGGG	1 cut(s)	100
SmiMI	CAYNNNNRTG	1 cut(s)	2224
SmlI	CTYRAG	4 cut(s)	322, 957, 1383, 2048
SmoI	CTYRAG	4 cut(s)	322, 957, 1383, 2048
SnaBI	TACGTA	1 cut(s)	1519
SseBI	AGGCCT	1 cut(s)	2254
SsiI	CCGC	7 cut(s)	285, 614, 767, 848, 940, 1357, 2364
SspI	AATATT	2 cut(s)	655, 1777
SspMI	CTAG	3 cut(s)	1331, 2171, 2376
SstI	GAGCTC	1 cut(s)	1530
StuI	AGGCCT	1 cut(s)	2254
StyI	CCWWGG	4 cut(s)	381, 609, 1495, 1570
TaaI	ACNGT	6 cut(s)	1418, 1451, 1954, 2030, 2090, 2408
TaiI	ACGT	5 cut(s)	534, 645, 1011, 1521, 1632
TaqI	TCGA	4 cut(s)	88, 155, 1023, 2160
TatI	WGTACW	4 cut(s)	1350, 1692, 1942, 2025
TauI	GCSGC	2 cut(s)	770, 2367
TfiI	GAWTC	5 cut(s)	90, 395, 1239, 1344, 1770
Tru1I	TTAA	4 cut(s)	945, 1226, 1286, 2124
Tru9I	TTAA	4 cut(s)	945, 1226, 1286, 2124
TscAI	CASTG	7 cut(s)	337, 792, 1788, 1795, 1847, 2088, 2413
TseFI	GTSAC	3 cut(s)	1603, 1625, 2200
TseI	GCWGC	3 cut(s)	502, 861, 1134
Tsp45I	GTSAC	3 cut(s)	1603, 1625, 2200
TspDTI	ATGAA	8 cut(s)	846, 1232, 1248, 1432, 1830, 1937, 2196, 2205
TspGWI	ACGGA	3 cut(s)	476, 1010, 2326
TspMI	CCCGGG	1 cut(s)	98
TspRI	CASTG	7 cut(s)	337, 792, 1788, 1795, 1847, 2088, 2413
Van91I	CCANNNNNTGG	1 cut(s)	193
VpaK11BI	GGWCC	2 cut(s)	606, 745
XagI	CCTNNNNNAGG	1 cut(s)	1574
XapI	RAATTY	7 cut(s)	549, 688, 853, 1192, 1474, 1620, 2071
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1502
XmaI	CCCGGG	1 cut(s)	98
XmiI	GTMKAC	3 cut(s)	350, 575, 1515
XmnI	GAANNNNTTC	3 cut(s)	755, 1863, 2153
XspI	CTAG	3 cut(s)	1331, 2171, 2376
Zsp2I	ATGCAT	3 cut(s)	757, 2122, 2247

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.