Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0008150

Physical Location & Seq

Genomic Coordinates Forward (+)

54390703 .. 54394334

Gene Structure 3632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG383800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGGGTTTGTTCCAGTTTAGAGCTTTGTGGCTCTTTATGATCCTCCTCCTTGAGTTGAATACCTGCATAGCTAGTCAGCAACATTCAACTAGGATTTATCCCGGGTTCCAGGCATCCCAGATGGAATGGATTGATAATTCGGGGCGGTTCCTGCTGTCCAACAACTCAGCATTTGCTCTTGGATTCTACCAAGGCCTTGATGTCCTTGAGGTCAAGTCGTTTTTACTGGTGATCATTCACTTGGGCACGTCCAAAGTGGTTTGGACTGCTAATAGAGACTTACTGGTTGCAAATTCTGATAAATTTGCCTTCAATAAAAATGGCAATGTCTACTTGGAAAGCGGCGATGATAAGGTTTGGTCTACCAACACAACGGGAGAAGTAGTTACAGCCATGGAATTGCGGGATTCGGGAAACTTGGTGTTGCTTGGAGAGAACGGAAGCATTCTTTGGCAGAGTTTTAGCCACCCGACTGATACTCTTTTACCGGGTCAGGAGTTCTTGGAAGGAATGAAACTCAAGAGCTTTCGGGACAAGAACAACTTGTCTAATTATCTCGAGATTCAGTCAGGCGAGTTGGTTCTGTATGCTGGATATCAAACTCCTCAGATCTATTGGTCAATAGCAGATGAGAGCAGGAAAAGCAATAACAATGTCAGTGGAAAGGTTCACTCTGTGTCTCTGGTGTCCAATTCATTGAATTTCTATTATCAAAGTAAATCCTTACTTTGGCAATTTGTTTTCTCCGACAACAAAGATCCGAATGCCTTCTGGGCCACTGTTTTAGGCCAGGATGGCTCAATCACCTTCCTTAATTTTCAGAAAGGAAGTTCTGTTGTCGCTGAAGCAACTAAGATCCCACAAGAGTCTTGCAGCCTGCCTGAGCGTTGTGAGCCTTACTTTGTGTGCTTTTTTGATGGTTGGTGCCAATGCCCCTCGCTTCTCACCTCTCGCTTTAATTGCAGACCTCCAGCAGTCCCAACATGTAGTACCTCCAAAAGTTCAGTGGACCTTCTTTATGTAGATCAGAAGCTTGATTATTTTGCGCTTGGTTTCACTAAACCCTCTTTGAAATCCAATTTGAGTTCCTGCAAAGAAGCTTGCCTTGGTGACTGTTCTTGCCTTGTTCTGTTCTTCGAAAACACTACTGGAAATTGTTTTCTGTTTGACCAGGTCGGGAATTTGCAACGTTCGGCTGCGAATAGTACTGGTTATACCTCATATATGAAGGTCTTTACTGAAGATGTAGGGCTAAGCCCTGCAAAGAAGGCAAACCGAAATGGGAAAATGCACATCCTTGTTATAGCGGTCATTGTGACTGTAACATTATTGGTGTGTGTTGGTCTAGTTTCTTTTGGATTATTGTACTACCGCAAGAAGAAGAGATTACTGGACTACTCTGAAGAAATCAGGGAAGAGGATACTTTCTTGGACAGTCTTTCAGGAATGCCTATTCGTTTCAGTTATAGCGATCTTAAAACAGCAACTAAAAATTTCTCCACAAAAATTGGCATAGGAGGGTTTGGTTCTGTTTACCTAGGTGAGCTTCCAGATGGCATCCGGCTTGCTGTGAAAAAGTTAGAGGGACAACTTCAGGGGAAGAAAGAGTTTAGAGCTGAAGTTACTATCATTGGGAAAATTCGACATGCGCATTTGGTCAAGCTGAGAGGCTTTTGTGCAGAAGGGCTTCACAGGCTTCTTGTCTATGAGTATATGGCACAAGGGTCTTTAGATAAATGGATATTCAACAAAAAAAATGATGACCATTTTTTGGATTGGAACGCAAGATTCAATATTGCATTGGGAACTGCAAAAGGGTTGGCTTATCTCCATGAAGAATGTGAAGAGAAGATTGTCCACTGTGATATAAAACCTGAAAATGTTCTTCTTGATGAAAATTTCAATGCCAAAGTTTCAGATTTTGGTTTGGCTAAGCTGATGAACCGGGACCAAAGCCTTGTACACACGACGTTGAGGGGCACGAGAGGGTACCTTGCACCAGAATGGCTCACCAATGCTGCCATATCAGAGAAAAGTGACGTTTATAGTTACGGCATGGTTTTGCTTGAGATCGTTGGTGGAAAAAGGAATTTTGCTCCCGGGGAGAGTTCAGGGAAATCTCACTTTCCTTCTCATGCCTTTAAACTTTTGGAAGAGGGAAACTTGAAAGAATTCATTGATCCAAAGTTAGATGTCGATGAAAAAGATGAGAAAGTAGTGGCTGCAATTGAAGTTGCATTGTGGTGTATTCAAGCTGAGATGCAGTTGAGACCCTCAATGACTAGAGTGGTCCAGATGCTTGAAGGTCTTTGTGATCTACCACCATCGCCGACATCCTCCCCACGGTCAGGTCCTCATTTGAGTTTTTTGCATTGGTGCAGCAAAGAAGTTACTTCGCCAGCAGGACTCAATGGTTACTATAGTGATATGCCCATGTCAGGTGTTCGGCTTTCAGGGCCAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_1 Pkinase

Protein Analysis

821

Amino Acids

91.76

Weight (kDa)

6.38

Isoelectric Point (pI)

33.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	86 - 168	1.3e-18	D-mannose binding lectin
PK_Tyr_Ser-Thr	PF07714	499 - 767	2.3e-42	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	500 - 765	4e-49	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008150)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g29821 FvH4_6g29840
malus_domestica	MD17G1210300.v1.1
prunus_persica	Prupe.3G085600_v2.0.a1
pyrus_communis	pycom17g21560
rosa_chinensis	RchiOBHm_Chr2g0135701
rosa_laevigata	RLG00000019478 RLG00000019479
rosa_multiflora	Rmu_co8459773.1_g000001 Rmu_sc0002398.1_g000047 Rmu_sc0007848.1_g000046
rosa_roxburghii	Rroxscaffold_2G00108980 Rroxscaffold_2G00109000
rosa_rugosa	Rorug02G0324900 Rorug02G0325100
rosa_samantha	Rh2AG377400 Rh2AG377500 Rh2BG383700 Rh2BG383800 Rh2CG362900 Rh2CG363000 Rh2DG400100
rosa_wichuraiana	Rw2G030740 Rw2G030750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1650
Acc36I	ACCTGC	1 cut(s)	71
Acc65I	GGTACC	1 cut(s)	1989
AccB1I	GGYRCC	2 cut(s)	924, 1989
AccB7I	CCANNNNNTGG	1 cut(s)	1771
AccI	GTMKAC	2 cut(s)	330, 362
AciI	CCGC	5 cut(s)	145, 342, 403, 1307, 1372
AclI	AACGTT	1 cut(s)	1189
AclWI	GGATC	4 cut(s)	34, 752, 850, 2174
AcsI	RAATTY	9 cut(s)	292, 302, 700, 1180, 1492, 1638, 1897, 2089, 2171
AcuI	CTGAAG	5 cut(s)	864, 1260, 1422, 1577, 1638
AdeI	CACNNNGTG	1 cut(s)	676
AfaI	GTAC	5 cut(s)	991, 1207, 1367, 1962, 1991
AfiI	CCNNNNNNNGG	5 cut(s)	729, 1771, 2343, 2348, 2438
AflIII	ACRYGT	1 cut(s)	983
AjiI	CACGTC	1 cut(s)	249
AjnI	CCWGG	3 cut(s)	108, 789, 1170
AjuI	GAANNNNNNNTTGG	4 cut(s)	433, 465, 1089, 1121
Alw26I	GTCTC	3 cut(s)	270, 684, 2263
AlwI	GGATC	4 cut(s)	34, 752, 850, 2174
Ama87I	CYCGRG	3 cut(s)	101, 557, 2099
AoxI	GGCC	4 cut(s)	193, 774, 787, 2456
ApeKI	GCWGC	5 cut(s)	873, 1196, 2018, 2222, 2379
ApoI	RAATTY	9 cut(s)	292, 302, 700, 1180, 1492, 1638, 1897, 2089, 2171
Asp700I	GAANNNNTTC	4 cut(s)	767, 1686, 1881, 2171
Asp718I	GGTACC	1 cut(s)	1989
AspA2I	CCTAGG	1 cut(s)	1537
AspLEI	GCGC	2 cut(s)	1048, 1651
AspS9I	GGNCC	6 cut(s)	774, 1009, 1948, 2290, 2351, 2456
AsuC2I	CCSGG	6 cut(s)	102, 103, 489, 1946, 2100, 2101
AsuHPI	GGTGA	6 cut(s)	241, 796, 937, 1121, 1553, 2002
AsuII	TTCGAA	1 cut(s)	1137
AvaI	CYCGRG	3 cut(s)	101, 557, 2099
AvaII	GGWCC	4 cut(s)	1009, 1948, 2290, 2351
AvrII	CCTAGG	1 cut(s)	1537
BaeGI	GKGCMC	2 cut(s)	248, 1982
BanI	GGYRCC	2 cut(s)	924, 1989
BauI	CACGAG	1 cut(s)	1981
BbvI	GCAGC	5 cut(s)	885, 1183, 2005, 2209, 2391
BccI	CCATC	5 cut(s)	115, 788, 911, 1547, 2332
BceAI	ACGGC	1 cut(s)	2068
BciT130I	CCWGG	3 cut(s)	110, 791, 1172
BciVI	GTATCC	1 cut(s)	1414
BclI	TGATCA	1 cut(s)	231
BcnI	CCSGG	6 cut(s)	102, 103, 489, 1946, 2100, 2101
BcoDI	GTCTC	3 cut(s)	270, 684, 2263
BfaI	CTAG	5 cut(s)	72, 90, 1346, 1538, 2283
BfmI	CTRYAG	1 cut(s)	2419
BfuAI	ACCTGC	1 cut(s)	71
BfuI	GTATCC	1 cut(s)	1414
BglI	GCCNNNNNGGC	2 cut(s)	773, 795
BglII	AGATCT	1 cut(s)	609
BisI	GCNGC	6 cut(s)	343, 874, 1197, 2019, 2223, 2380
BlnI	CCTAGG	1 cut(s)	1537
BlpI	GCTNAGC	2 cut(s)	1253, 1932
BlsI	GCNGC	6 cut(s)	344, 875, 1198, 2020, 2224, 2381
BmcAI	AGTACT	1 cut(s)	1207
Bme1390I	CCNGG	9 cut(s)	102, 103, 110, 489, 791, 1172, 1946, 2100, 2101
Bme18I	GGWCC	4 cut(s)	1009, 1948, 2290, 2351
BmeT110I	CYCGRG	3 cut(s)	101, 557, 2099
BmgBI	CACGTC	1 cut(s)	249
BmgT120I	GGNCC	6 cut(s)	774, 1009, 1948, 2290, 2351, 2456
BmiI	GGNNCC	5 cut(s)	107, 149, 926, 1949, 1991
BmrFI	CCNGG	9 cut(s)	102, 103, 110, 489, 791, 1172, 1946, 2100, 2101
BmsI	GCATC	4 cut(s)	122, 1566, 2250, 2286
BpmI	CTGGAG	1 cut(s)	954
Bpu10I	CCTNAGC	1 cut(s)	882
Bpu1102I	GCTNAGC	2 cut(s)	1253, 1932
Bpu14I	TTCGAA	1 cut(s)	1137
BpuEI	CTTGAG	4 cut(s)	71, 227, 503, 2087
BpuMI	CCSGG	6 cut(s)	102, 103, 489, 1946, 2100, 2101
BsaI	GGTCTC	1 cut(s)	2263
BsaJI	CCNNGG	8 cut(s)	101, 190, 393, 1105, 1537, 2099, 2100, 2342
BsaXI	ACNNNNNCTCC	2 cut(s)	369, 399
Bsc4I	CCNNNNNNNGG	5 cut(s)	729, 1771, 2343, 2348, 2438
Bse1I	ACTGG	6 cut(s)	13, 231, 288, 1153, 1213, 1395
Bse3DI	GCAATG	1 cut(s)	331
BseBI	CCWGG	3 cut(s)	110, 791, 1172
BseDI	CCNNGG	8 cut(s)	101, 190, 393, 1105, 1537, 2099, 2100, 2342
BseGI	GGATG	5 cut(s)	113, 799, 1293, 1557, 2333
BseLI	CCNNNNNNNGG	5 cut(s)	729, 1771, 2343, 2348, 2438
BseMI	GCAATG	1 cut(s)	331
BseMII	CTCAG	5 cut(s)	180, 620, 873, 1655, 2247
BseNI	ACTGG	6 cut(s)	13, 231, 288, 1153, 1213, 1395
BseRI	GAGGAG	2 cut(s)	35, 594
BseSI	GKGCMC	2 cut(s)	248, 1982
BseXI	GCAGC	5 cut(s)	885, 1183, 2005, 2209, 2391
BsgI	GTGCAG	2 cut(s)	1698, 2398
BshFI	GGCC	4 cut(s)	195, 776, 789, 2458
BshNI	GGYRCC	2 cut(s)	924, 1989
BsiHKCI	CYCGRG	3 cut(s)	101, 557, 2099
BsiSI	CCGG	5 cut(s)	102, 488, 1561, 1945, 2100
BslFI	GGGAC	4 cut(s)	545, 962, 1599, 1961
BslI	CCNNNNNNNGG	5 cut(s)	729, 1771, 2343, 2348, 2438
BsmAI	GTCTC	3 cut(s)	270, 684, 2263
BsmFI	GGGAC	4 cut(s)	545, 962, 1599, 1961
BsmI	GAATGC	3 cut(s)	444, 769, 1452
BsnI	GGCC	4 cut(s)	195, 776, 789, 2458
Bso31I	GGTCTC	1 cut(s)	2263
BsoBI	CYCGRG	3 cut(s)	101, 557, 2099
Bsp119I	TTCGAA	1 cut(s)	1137
Bsp1286I	GDGCHC	2 cut(s)	248, 1982
Bsp1407I	TGTACA	1 cut(s)	1960
Bsp1720I	GCTNAGC	2 cut(s)	1253, 1932
Bsp19I	CCATGG	1 cut(s)	393
BspACI	CCGC	5 cut(s)	145, 342, 403, 1307, 1372
BspANI	GGCC	4 cut(s)	195, 776, 789, 2458
BspCNI	CTCAG	5 cut(s)	179, 619, 874, 1656, 2248
BspLI	GGNNCC	5 cut(s)	107, 149, 926, 1949, 1991
BspMI	ACCTGC	1 cut(s)	71
BspPI	GGATC	4 cut(s)	34, 752, 850, 2174
BspT104I	TTCGAA	1 cut(s)	1137
BspT107I	GGYRCC	2 cut(s)	924, 1989
BspTNI	GGTCTC	1 cut(s)	2263
BsrDI	GCAATG	1 cut(s)	331
BsrGI	TGTACA	1 cut(s)	1960
BsrI	ACTGG	6 cut(s)	13, 231, 288, 1153, 1213, 1395
BssECI	CCNNGG	8 cut(s)	101, 190, 393, 1105, 1537, 2099, 2100, 2342
BssSI	CACGAG	1 cut(s)	1981
BssT1I	CCWWGG	4 cut(s)	190, 393, 1105, 1537
Bst2BI	CACGAG	1 cut(s)	1981
Bst2UI	CCWGG	3 cut(s)	110, 791, 1172
Bst4CI	ACNGT	6 cut(s)	781, 1115, 1321, 1436, 1862, 2346
Bst6I	CTCTTC	4 cut(s)	1376, 1410, 1839, 2148
BstAUI	TGTACA	1 cut(s)	1960
BstBI	TTCGAA	1 cut(s)	1137
BstC8I	GCNNGC	4 cut(s)	878, 1102, 1566, 2400
BstDEI	CTNAG	8 cut(s)	166, 606, 852, 882, 1253, 1664, 1932, 2256
BstDSI	CCRYGG	2 cut(s)	393, 2342
BstF5I	GGATG	5 cut(s)	113, 799, 1293, 1557, 2333
BstHHI	GCGC	2 cut(s)	1048, 1651
BstMAI	GTCTC	3 cut(s)	270, 684, 2263
BstMWI	GCNNNNNNNGC	9 cut(s)	151, 642, 773, 795, 892, 960, 1268, 1693, 2455
BstNI	CCWGG	3 cut(s)	110, 791, 1172
BstNSI	RCATGY	2 cut(s)	987, 1649
BstSCI	CCNGG	9 cut(s)	100, 101, 108, 487, 789, 1170, 1944, 2098, 2099
BstSFI	CTRYAG	1 cut(s)	2419
BstSLI	GKGCMC	2 cut(s)	248, 1982
BstV1I	GCAGC	5 cut(s)	885, 1183, 2005, 2209, 2391
BstX2I	RGATCY	3 cut(s)	609, 757, 855
BstYI	RGATCY	3 cut(s)	609, 757, 855
BsuI	GTATCC	1 cut(s)	1414
BsuRI	GGCC	4 cut(s)	195, 776, 789, 2458
BtgI	CCRYGG	2 cut(s)	393, 2342
BtgZI	GCGATG	2 cut(s)	360, 2310
BtrI	CACGTC	1 cut(s)	249
BtsCI	GGATG	5 cut(s)	113, 799, 1293, 1557, 2333
BtsIMutI	CAGTG	4 cut(s)	664, 777, 1011, 1858
BveI	ACCTGC	1 cut(s)	71
Cac8I	GCNNGC	4 cut(s)	878, 1102, 1566, 2400
CfoI	GCGC	2 cut(s)	1048, 1651
Cfr13I	GGNCC	6 cut(s)	774, 1009, 1948, 2290, 2351, 2456
Cfr9I	CCCGGG	2 cut(s)	101, 2099
CsiI	ACCWGGT	1 cut(s)	1170
Csp6I	GTAC	5 cut(s)	990, 1206, 1366, 1961, 1990
CspCI	CAANNNNNGTGG	4 cut(s)	640, 675, 1489, 1524
CviAII	CATG	7 cut(s)	394, 984, 1646, 1832, 2056, 2135, 2434
CviQI	GTAC	5 cut(s)	990, 1206, 1366, 1961, 1990
DdeI	CTNAG	8 cut(s)	166, 606, 852, 882, 1253, 1664, 1932, 2256
DraI	TTTAAA	1 cut(s)	2143
DraIII	CACNNNGTG	1 cut(s)	676
Eam1104I	CTCTTC	4 cut(s)	1376, 1410, 1839, 2148
EarI	CTCTTC	4 cut(s)	1376, 1410, 1839, 2148
Eco130I	CCWWGG	4 cut(s)	190, 393, 1105, 1537
Eco147I	AGGCCT	1 cut(s)	195
Eco31I	GGTCTC	1 cut(s)	2263
Eco32I	GATATC	1 cut(s)	596
Eco47I	GGWCC	4 cut(s)	1009, 1948, 2290, 2351
Eco57I	CTGAAG	5 cut(s)	864, 1260, 1422, 1577, 1638
Eco88I	CYCGRG	3 cut(s)	101, 557, 2099
EcoO109I	RGGNCCY	1 cut(s)	2351
EcoRI	GAATTC	1 cut(s)	2171
EcoRII	CCWGG	3 cut(s)	108, 789, 1170
EcoRV	GATATC	1 cut(s)	596
EcoT14I	CCWWGG	4 cut(s)	190, 393, 1105, 1537
ErhI	CCWWGG	4 cut(s)	190, 393, 1105, 1537
FaeI	CATG	7 cut(s)	397, 987, 1649, 1835, 2059, 2138, 2437
FalI	AAGNNNNNCTT	4 cut(s)	527, 559, 1089, 1121
FaqI	GGGAC	4 cut(s)	545, 962, 1599, 1961
FatI	CATG	7 cut(s)	393, 983, 1645, 1831, 2055, 2134, 2433
FauI	CCCGC	1 cut(s)	396
FbaI	TGATCA	1 cut(s)	231
FblI	GTMKAC	2 cut(s)	330, 362
Fnu4HI	GCNGC	6 cut(s)	343, 874, 1197, 2019, 2223, 2380
FokI	GGATG	5 cut(s)	100, 806, 1280, 1544, 2320
Fsp4HI	GCNGC	6 cut(s)	343, 874, 1197, 2019, 2223, 2380
FspAI	RTGCGCAY	1 cut(s)	1650
FspBI	CTAG	5 cut(s)	72, 90, 1346, 1538, 2283
FspI	TGCGCA	1 cut(s)	1650
GlaI	GCGC	2 cut(s)	1047, 1650
GluI	GCNGC	6 cut(s)	343, 874, 1197, 2019, 2223, 2380
GsuI	CTGGAG	1 cut(s)	954
HaeIII	GGCC	4 cut(s)	195, 776, 789, 2458
HapII	CCGG	5 cut(s)	102, 488, 1561, 1945, 2100
HhaI	GCGC	2 cut(s)	1048, 1651
Hin1II	CATG	7 cut(s)	397, 987, 1649, 1835, 2059, 2138, 2437
Hin6I	GCGC	2 cut(s)	1046, 1649
HinP1I	GCGC	2 cut(s)	1046, 1649
HindIII	AAGCTT	2 cut(s)	1031, 1098
HinfI	GANTC	6 cut(s)	183, 407, 562, 866, 1788, 2406
HpaII	CCGG	5 cut(s)	102, 488, 1561, 1945, 2100
HphI	GGTGA	6 cut(s)	241, 796, 937, 1121, 1553, 2002
Hpy166II	GTNNAC	7 cut(s)	331, 363, 670, 1009, 1534, 1858, 1963
Hpy188I	TCNGA	9 cut(s)	298, 609, 748, 762, 822, 1029, 1402, 1918, 2029
Hpy8I	GTNNAC	7 cut(s)	331, 363, 670, 1009, 1534, 1858, 1963
Hpy99I	CGWCG	1 cut(s)	1972
HpyCH4III	ACNGT	6 cut(s)	781, 1115, 1321, 1436, 1862, 2346
HpyCH4IV	ACGT	4 cut(s)	248, 1189, 1970, 2040
HpyF10VI	GCNNNNNNNGC	9 cut(s)	151, 642, 773, 795, 892, 960, 1268, 1693, 2455
HpyF3I	CTNAG	8 cut(s)	166, 606, 852, 882, 1253, 1664, 1932, 2256
HpySE526I	ACGT	4 cut(s)	248, 1189, 1970, 2040
Hsp92II	CATG	7 cut(s)	397, 987, 1649, 1835, 2059, 2138, 2437
HspAI	GCGC	2 cut(s)	1046, 1649
KpnI	GGTACC	1 cut(s)	1993
Ksp22I	TGATCA	1 cut(s)	231
LmnI	GCTCC	1 cut(s)	2101
Lsp1109I	GCAGC	5 cut(s)	885, 1183, 2005, 2209, 2391
LweI	GCATC	4 cut(s)	122, 1566, 2250, 2286
MabI	ACCWGGT	1 cut(s)	1170
MaeI	CTAG	5 cut(s)	72, 90, 1346, 1538, 2283
MaeII	ACGT	4 cut(s)	248, 1189, 1970, 2040
MaeIII	GTNAC	9 cut(s)	385, 1109, 1315, 1321, 1621, 2036, 2048, 2389, 2414
MfeI	CAATTG	1 cut(s)	2226
MflI	RGATCY	3 cut(s)	609, 757, 855
MhlI	GDGCHC	2 cut(s)	248, 1982
MlyI	GAGTC	2 cut(s)	875, 2400
MmeI	TCCRAC	2 cut(s)	183, 771
MroXI	GAANNNNTTC	4 cut(s)	767, 1686, 1881, 2171
MseI	TTAA	4 cut(s)	813, 957, 1476, 2142
MslI	CAYNNNNRTG	2 cut(s)	2002, 2242
MspI	CCGG	5 cut(s)	102, 488, 1561, 1945, 2100
MspR9I	CCNGG	9 cut(s)	102, 103, 110, 489, 791, 1172, 1946, 2100, 2101
MunI	CAATTG	1 cut(s)	2226
Mva1269I	GAATGC	3 cut(s)	444, 769, 1452
MvaI	CCWGG	3 cut(s)	110, 791, 1172
MwoI	GCNNNNNNNGC	9 cut(s)	151, 642, 773, 795, 892, 960, 1268, 1693, 2455
NciI	CCSGG	6 cut(s)	102, 103, 489, 1946, 2100, 2101
NcoI	CCATGG	1 cut(s)	393
NlaIII	CATG	7 cut(s)	397, 987, 1649, 1835, 2059, 2138, 2437
NlaIV	GGNNCC	5 cut(s)	107, 149, 926, 1949, 1991
NmuCI	GTSAC	3 cut(s)	1109, 1315, 2036
NsbI	TGCGCA	1 cut(s)	1650
NspI	RCATGY	2 cut(s)	987, 1649
NspV	TTCGAA	1 cut(s)	1137
PaeR7I	CTCGAG	1 cut(s)	557
PceI	AGGCCT	1 cut(s)	195
PciI	ACATGT	1 cut(s)	983
PctI	GAATGC	3 cut(s)	444, 769, 1452
PdmI	GAANNNNTTC	4 cut(s)	767, 1686, 1881, 2171
PfeI	GAWTC	4 cut(s)	183, 407, 562, 1788
PflFI	GACNNNGTC	1 cut(s)	1172
PflMI	CCANNNNNTGG	1 cut(s)	1771
PkrI	GCNGC	6 cut(s)	344, 875, 1198, 2020, 2224, 2381
PleI	GAGTC	2 cut(s)	874, 2400
PpsI	GAGTC	2 cut(s)	874, 2400
PpuMI	RGGWCCY	1 cut(s)	2351
PscI	ACATGT	1 cut(s)	983
Psp1406I	AACGTT	1 cut(s)	1189
Psp5II	RGGWCCY	1 cut(s)	2351
Psp6I	CCWGG	3 cut(s)	108, 789, 1170
PspGI	CCWGG	3 cut(s)	108, 789, 1170
PspN4I	GGNNCC	5 cut(s)	107, 149, 926, 1949, 1991
PspPI	GGNCC	6 cut(s)	774, 1009, 1948, 2290, 2351, 2456
PspPPI	RGGWCCY	1 cut(s)	2351
PsuI	RGATCY	3 cut(s)	609, 757, 855
PsyI	GACNNNGTC	1 cut(s)	1172
RsaI	GTAC	5 cut(s)	991, 1207, 1367, 1962, 1991
RsaNI	GTAC	5 cut(s)	990, 1206, 1366, 1961, 1990
RseI	CAYNNNNRTG	2 cut(s)	2002, 2242
SaqAI	TTAA	4 cut(s)	813, 957, 1476, 2142
SatI	GCNGC	6 cut(s)	343, 874, 1197, 2019, 2223, 2380
Sau96I	GGNCC	6 cut(s)	774, 1009, 1948, 2290, 2351, 2456
ScaI	AGTACT	1 cut(s)	1207
SchI	GAGTC	2 cut(s)	875, 2400
ScrFI	CCNGG	9 cut(s)	102, 103, 110, 489, 791, 1172, 1946, 2100, 2101
SduI	GDGCHC	2 cut(s)	248, 1982
SexAI	ACCWGGT	1 cut(s)	1170
SfaNI	GCATC	4 cut(s)	122, 1566, 2250, 2286
SfcI	CTRYAG	1 cut(s)	2419
Sfr274I	CTCGAG	1 cut(s)	557
SfuI	TTCGAA	1 cut(s)	1137
SinI	GGWCC	4 cut(s)	1009, 1948, 2290, 2351
SlaI	CTCGAG	1 cut(s)	557
SmaI	CCCGGG	2 cut(s)	103, 2101
SmiMI	CAYNNNNRTG	2 cut(s)	2002, 2242
SmlI	CTYRAG	5 cut(s)	50, 206, 518, 557, 2066
SmoI	CTYRAG	5 cut(s)	50, 206, 518, 557, 2066
SseBI	AGGCCT	1 cut(s)	195
SsiI	CCGC	5 cut(s)	145, 342, 403, 1307, 1372
SspI	AATATT	1 cut(s)	1795
SspMI	CTAG	5 cut(s)	72, 90, 1346, 1538, 2283
StuI	AGGCCT	1 cut(s)	195
StyD4I	CCNGG	9 cut(s)	100, 101, 108, 487, 789, 1170, 1944, 2098, 2099
StyI	CCWWGG	4 cut(s)	190, 393, 1105, 1537
TaaI	ACNGT	6 cut(s)	781, 1115, 1321, 1436, 1862, 2346
TaiI	ACGT	4 cut(s)	251, 1192, 1973, 2043
TaqI	TCGA	4 cut(s)	558, 1137, 1642, 2196
TatI	WGTACW	3 cut(s)	1205, 1365, 1960
TauI	GCSGC	1 cut(s)	345
TfiI	GAWTC	4 cut(s)	183, 407, 562, 1788
Tru1I	TTAA	4 cut(s)	813, 957, 1476, 2142
Tru9I	TTAA	4 cut(s)	813, 957, 1476, 2142
TscAI	CASTG	4 cut(s)	664, 784, 1011, 1865
TseFI	GTSAC	3 cut(s)	1109, 1315, 2036
TseI	GCWGC	5 cut(s)	873, 1196, 2018, 2222, 2379
Tsp45I	GTSAC	3 cut(s)	1109, 1315, 2036
TspDTI	ATGAA	8 cut(s)	527, 684, 1241, 1848, 1908, 1955, 2164, 2214
TspGWI	ACGGA	1 cut(s)	453
TspMI	CCCGGG	2 cut(s)	101, 2099
TspRI	CASTG	4 cut(s)	664, 784, 1011, 1865
Tth111I	GACNNNGTC	1 cut(s)	1172
Van91I	CCANNNNNTGG	1 cut(s)	1771
VpaK11BI	GGWCC	4 cut(s)	1009, 1948, 2290, 2351
XapI	RAATTY	9 cut(s)	292, 302, 700, 1180, 1492, 1638, 1897, 2089, 2171
XceI	RCATGY	2 cut(s)	987, 1649
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1003
XhoI	CTCGAG	1 cut(s)	557
XmaI	CCCGGG	2 cut(s)	101, 2099
XmaJI	CCTAGG	1 cut(s)	1537
XmiI	GTMKAC	2 cut(s)	330, 362
XmnI	GAANNNNTTC	4 cut(s)	767, 1686, 1881, 2171
XspI	CTAG	5 cut(s)	72, 90, 1346, 1538, 2283
ZrmI	AGTACT	1 cut(s)	1207

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.