Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0016093

Physical Location & Seq

Genomic Coordinates Forward (+)

26438513 .. 26439997

Gene Structure 1485 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1217500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1485 bp

ATGGATAGCAACTATTCAGCTATCCCTAAGAAGTCGTACGTGGAATTGCAATCGCAGTATGATTCTGAAAACCCAGAAAATCTTTCAAAATCCCATTCCAAATTATCGGATTTTGATGAGGAAAGCTTTCTAGATTCCAAGAATTTGAATGGGAATGATGGCGGTGATGATTTCGATGATAATCTTGATTTCGATATTGGTAGTTATCCTTTAATTGTTGAGGGGTCTAATAATGGGTCTGGAATCTCCGGGGCGGTGTTTAATCTCACGACATCGATCATTGGGGCTGGGATTATGGCTTTACCTGCTACCATGAAGGTTCTTGGATTGGTTTGGGGGTTTGTTTTGATAATTTTGATGGGTATTTTGTCAGAAATTAGTGTTGAATTGTTGGTGAGGTTTTCGGTTTTGTGTAAGGCATCGTCTTATGGGGAGGTTGTTCAATGTGCACTGGGTAGGCCCTGGAGGATTCTGTCGGAGATCTGCATAATTATCAATAATGCAGGGGTGTTGGTGGTGTATTTGATAATTATGGGTGATGTTATGTCTGGTTCACTCCATCATATTGGGGTTTTCGATCAATGGTTAGGACATGGGGTTTGGGATCATCGAATGCTTCTGATTTTTGCTGTGGTGGTGGTTTGTCTGGCACCACTTTGCGCCTTGGAGAAGATTGACTCCTTGAGCTTGACTTCAGCTGCTTCGGTAGCTCTTGCAGTTGTGTTTGTTGTAGTTGCTTGCGCTATCGCGTTTATTAAGCTTGTTGAAGGGAAAATTGATTCTCCAAGGATGTCACCGGATTTTGGGTCGACCAAGGCAATCTTGGATTTGCTTGTGGTGATTCCTATCATGACCAATGCTTATGTTTGTCACTTTAATGTTCAACCTATATACAATGAGCTTGAAGGCCGGTCTCCTCAAAAGATGAATCGGGTTGGAAGGATCACAACTGTTCTTTGCATTGTGATCTATTCTTTGACAGCCATTGCAGGATATCTACTCTTTGGGAAAGATACAGAGTCTGATATTCTGACCAACTTTGATAAAGACCTTGGAATCCGTTTCAGCTCAGCACTGAATTATATTGTCAGGGTTGGGTACATTTTCCATTTGCTTCTTGTTTTCCCTGTTGTCCATTTTTCCTTGAGGCAAACAGTGGACGCCTTGGTGTTTGAGGGATCACCTCCTCTCATGGAGAGTAGGAAGAGATGTTTTGTATTGACACTGGTTCTGCTGGGGCTTATTTATTTAGGATCTACCGTGATTCCCAACATTTGGACAGCATTTAAGTTTACAGGGGCAACAACTGCCGTGTCATTGGGGTTTATATTTCCTTCCCTCATTGCTTTAAAGTTAGGTCGGAAAGGGGAAGGCTTGAGCGTTGTAGAGAAGACCTTGTCATGGTTCATGTTGGTAATGGCAGTAGCAGTCAGCATTGTTGGAATTATTGGCAATATTTATAGTCTCAGAAGCAAATCTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0012505 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015849 GO:0016020 GO:0022857 GO:0031090 GO:0031984 GO:0034220 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0098588 GO:0098656 GO:0098805 GO:0098827 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

ko04727 map04727

Pfam Domains

Protein Families

Aa_trans

Protein Analysis

495

Amino Acids

53.79

Weight (kDa)

5.46

Isoelectric Point (pI)

32.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aa_trans	PF01490	82 - 486	3.1e-59	Transmembrane amino acid transporter protein
Trp_Tyr_perm	PF03222	82 - 459	1.1e-07	Tryptophan/tyrosine permease family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016093)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80510
fragaria_vesca	FvH4_6g29140
malus_domestica	MD17G1217500.v1.1
prunus_persica	Prupe.3G093200_v2.0.a1
pyrus_communis	pycom17g22180
rosa_chinensis	RchiOBHm_Chr2g0134331
rosa_laevigata	RLG00000019386
rosa_multiflora	Rmu_sc0014586.1_g000002
rosa_roxburghii	Rroxscaffold_2G00110320
rosa_rugosa	Rorug02G0316100
rosa_samantha	Rh2AG368800 Rh2BG374800 Rh2CG352500 Rh2DG392100
rosa_wichuraiana	Rw2G030050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	313
AccB1I	GGYRCC	1 cut(s)	649
AccB7I	CCANNNNNTGG	1 cut(s)	1275
AccI	GTMKAC	1 cut(s)	809
AccII	CGCG	1 cut(s)	749
AciI	CCGC	2 cut(s)	162, 254
AclWI	GGATC	4 cut(s)	612, 950, 1186, 1261
AcsI	RAATTY	1 cut(s)	142
AcuI	CTGAAG	1 cut(s)	678
AcyI	GRCGYC	1 cut(s)	1161
AfaI	GTAC	2 cut(s)	38, 1100
AfiI	CCNNNNNNNGG	3 cut(s)	803, 1275, 1401
AgsI	TTSAA	7 cut(s)	87, 148, 386, 443, 767, 884, 905
AjnI	CCWGG	1 cut(s)	461
AluBI	AGCT	8 cut(s)	20, 126, 687, 698, 710, 760, 901, 1068
AluI	AGCT	8 cut(s)	20, 126, 687, 698, 710, 760, 901, 1068
Alw21I	GWGCWC	1 cut(s)	451
Alw26I	GTCTC	2 cut(s)	918, 1469
Alw44I	GTGCAC	1 cut(s)	447
AlwI	GGATC	4 cut(s)	612, 950, 1186, 1261
AlwNI	CAGNNNCTG	1 cut(s)	1022
AoxI	GGCC	2 cut(s)	458, 907
ApaLI	GTGCAC	1 cut(s)	447
ApeKI	GCWGC	1 cut(s)	698
ApoI	RAATTY	1 cut(s)	142
ArsI	GACNNNNNNTTYG	2 cut(s)	582, 614
Asp700I	GAANNNNTTC	1 cut(s)	126
AspLEI	GCGC	2 cut(s)	662, 743
AspS9I	GGNCC	1 cut(s)	459
AsuC2I	CCSGG	1 cut(s)	250
AsuHPI	GGTGA	6 cut(s)	176, 406, 548, 786, 850, 1173
BaeGI	GKGCMC	1 cut(s)	451
BanI	GGYRCC	1 cut(s)	649
BbsI	GAAGAC	1 cut(s)	1397
Bbv12I	GWGCWC	1 cut(s)	451
BbvI	GCAGC	1 cut(s)	685
BccI	CCATC	3 cut(s)	152, 352, 567
BceAI	ACGGC	1 cut(s)	1295
BciT130I	CCWGG	1 cut(s)	463
BcnI	CCSGG	1 cut(s)	250
BcoDI	GTCTC	2 cut(s)	918, 1469
BfaI	CTAG	1 cut(s)	131
BfuAI	ACCTGC	1 cut(s)	313
BglII	AGATCT	1 cut(s)	480
BisI	GCNGC	1 cut(s)	699
BlpI	GCTNAGC	1 cut(s)	1069
BlsI	GCNGC	1 cut(s)	700
Bme1390I	CCNGG	2 cut(s)	250, 463
BmgT120I	GGNCC	1 cut(s)	459
BmiI	GGNNCC	1 cut(s)	651
BmrFI	CCNGG	2 cut(s)	250, 463
BmrI	ACTGGG	1 cut(s)	461
BmsI	GCATC	1 cut(s)	428
BmuI	ACTGGG	1 cut(s)	461
BpiI	GAAGAC	1 cut(s)	1397
BpmI	CTGGAG	1 cut(s)	484
Bpu1102I	GCTNAGC	1 cut(s)	1069
BpuEI	CTTGAG	3 cut(s)	703, 1165, 1396
BpuMI	CCSGG	1 cut(s)	250
Bsa29I	ATCGAT	1 cut(s)	275
BsaAI	YACGTR	1 cut(s)	40
BsaBI	GATNNNNATC	2 cut(s)	180, 845
BsaHI	GRCGYC	1 cut(s)	1161
BsaI	GGTCTC	1 cut(s)	918
BsaJI	CCNNGG	7 cut(s)	249, 461, 663, 785, 813, 1051, 1164
BsaWI	WCCGGW	1 cut(s)	796
Bsc4I	CCNNNNNNNGG	3 cut(s)	803, 1275, 1401
Bse118I	RCCGGY	1 cut(s)	909
Bse1I	ACTGG	2 cut(s)	456, 1230
Bse3DI	GCAATG	2 cut(s)	984, 1341
Bse8I	GATNNNNATC	2 cut(s)	180, 845
BseBI	CCWGG	1 cut(s)	463
BseCI	ATCGAT	1 cut(s)	275
BseDI	CCNNGG	7 cut(s)	249, 461, 663, 785, 813, 1051, 1164
BseGI	GGATG	1 cut(s)	795
BseJI	GATNNNNATC	2 cut(s)	180, 845
BseLI	CCNNNNNNNGG	3 cut(s)	803, 1275, 1401
BseMI	GCAATG	2 cut(s)	984, 1341
BseMII	CTCAG	2 cut(s)	1083, 1480
BseNI	ACTGG	2 cut(s)	456, 1230
BseRI	GAGGAG	2 cut(s)	906, 1176
BseSI	GKGCMC	1 cut(s)	451
BseXI	GCAGC	1 cut(s)	685
BseYI	CCCAGC	2 cut(s)	287, 1234
Bsh1236I	CGCG	1 cut(s)	749
BshFI	GGCC	2 cut(s)	460, 909
BshNI	GGYRCC	1 cut(s)	649
BshVI	ATCGAT	1 cut(s)	275
BsiHKAI	GWGCWC	1 cut(s)	451
BsiSI	CCGG	3 cut(s)	249, 797, 910
BsiWI	CGTACG	1 cut(s)	36
BslI	CCNNNNNNNGG	3 cut(s)	803, 1275, 1401
BsmAI	GTCTC	2 cut(s)	918, 1469
BsmI	GAATGC	1 cut(s)	618
BsnI	GGCC	2 cut(s)	460, 909
Bso31I	GGTCTC	1 cut(s)	918
Bsp1286I	GDGCHC	1 cut(s)	451
Bsp143I	GATC	8 cut(s)	276, 480, 577, 604, 942, 966, 1178, 1253
Bsp1720I	GCTNAGC	1 cut(s)	1069
BspACI	CCGC	2 cut(s)	162, 254
BspANI	GGCC	2 cut(s)	460, 909
BspCNI	CTCAG	2 cut(s)	1082, 1479
BspDI	ATCGAT	1 cut(s)	275
BspFNI	CGCG	1 cut(s)	749
BspHI	TCATGA	1 cut(s)	849
BspLI	GGNNCC	1 cut(s)	651
BspMI	ACCTGC	1 cut(s)	313
BspPI	GGATC	4 cut(s)	612, 950, 1186, 1261
BspT107I	GGYRCC	1 cut(s)	649
BspTNI	GGTCTC	1 cut(s)	918
BsrDI	GCAATG	2 cut(s)	984, 1341
BsrFI	RCCGGY	1 cut(s)	909
BsrI	ACTGG	2 cut(s)	456, 1230
BssAI	RCCGGY	1 cut(s)	909
BssECI	CCNNGG	7 cut(s)	249, 461, 663, 785, 813, 1051, 1164
BssMI	GATC	8 cut(s)	276, 480, 577, 604, 942, 966, 1178, 1253
BssNI	GRCGYC	1 cut(s)	1161
BssT1I	CCWWGG	5 cut(s)	663, 785, 813, 1051, 1164
Bst2UI	CCWGG	1 cut(s)	463
Bst4CI	ACNGT	3 cut(s)	952, 1156, 1261
Bst6I	CTCTTC	1 cut(s)	1199
BstACI	GRCGYC	1 cut(s)	1161
BstAPI	GCANNNNNTGC	1 cut(s)	1307
BstBAI	YACGTR	1 cut(s)	40
BstC8I	GCNNGC	1 cut(s)	739
BstDEI	CTNAG	3 cut(s)	27, 1069, 1466
BstF5I	GGATG	1 cut(s)	795
BstFNI	CGCG	1 cut(s)	749
BstHHI	GCGC	2 cut(s)	662, 743
BstKTI	GATC	8 cut(s)	279, 483, 580, 607, 945, 969, 1181, 1256
BstMAI	GTCTC	2 cut(s)	918, 1469
BstMBI	GATC	8 cut(s)	276, 480, 577, 604, 942, 966, 1178, 1253
BstMWI	GCNNNNNNNGC	3 cut(s)	305, 707, 1307
BstNI	CCWGG	1 cut(s)	463
BstSCI	CCNGG	2 cut(s)	248, 461
BstSLI	GKGCMC	1 cut(s)	451
BstUI	CGCG	1 cut(s)	749
BstV1I	GCAGC	1 cut(s)	685
BstV2I	GAAGAC	1 cut(s)	1397
BstX2I	RGATCY	2 cut(s)	480, 1253
BstXI	CCANNNNNNTGG	1 cut(s)	566
BstYI	RGATCY	2 cut(s)	480, 1253
Bsu15I	ATCGAT	1 cut(s)	275
BsuRI	GGCC	2 cut(s)	460, 909
BsuTUI	ATCGAT	1 cut(s)	275
BtsCI	GGATG	1 cut(s)	795
BtsIMutI	CAGTG	4 cut(s)	449, 1073, 1161, 1223
BveI	ACCTGC	1 cut(s)	313
Cac8I	GCNNGC	1 cut(s)	739
CaiI	CAGNNNCTG	1 cut(s)	1022
CciI	TCATGA	1 cut(s)	849
CfoI	GCGC	2 cut(s)	662, 743
Cfr10I	RCCGGY	1 cut(s)	909
Cfr13I	GGNCC	1 cut(s)	459
ClaI	ATCGAT	1 cut(s)	275
CseI	GACGC	1 cut(s)	1169
Csp6I	GTAC	2 cut(s)	37, 1099
CviAII	CATG	6 cut(s)	313, 593, 850, 1192, 1401, 1408
CviQI	GTAC	2 cut(s)	37, 1099
DdeI	CTNAG	3 cut(s)	27, 1069, 1466
DpnI	GATC	8 cut(s)	278, 482, 579, 606, 944, 968, 1180, 1255
DpnII	GATC	8 cut(s)	276, 480, 577, 604, 942, 966, 1178, 1253
DraI	TTTAAA	1 cut(s)	1350
Eam1104I	CTCTTC	1 cut(s)	1199
EarI	CTCTTC	1 cut(s)	1199
Eco130I	CCWWGG	5 cut(s)	663, 785, 813, 1051, 1164
Eco31I	GGTCTC	1 cut(s)	918
Eco32I	GATATC	1 cut(s)	995
Eco57I	CTGAAG	1 cut(s)	678
EcoO109I	RGGNCCY	1 cut(s)	459
EcoRII	CCWGG	1 cut(s)	461
EcoRV	GATATC	1 cut(s)	995
EcoT14I	CCWWGG	5 cut(s)	663, 785, 813, 1051, 1164
ErhI	CCWWGG	5 cut(s)	663, 785, 813, 1051, 1164
FaeI	CATG	6 cut(s)	316, 596, 853, 1195, 1404, 1411
FalI	AAGNNNNNCTT	2 cut(s)	806, 838
FatI	CATG	6 cut(s)	312, 592, 849, 1191, 1400, 1407
FblI	GTMKAC	1 cut(s)	809
Fnu4HI	GCNGC	1 cut(s)	699
FokI	GGATG	1 cut(s)	802
Fsp4HI	GCNGC	1 cut(s)	699
FspBI	CTAG	1 cut(s)	131
GlaI	GCGC	2 cut(s)	661, 742
GluI	GCNGC	1 cut(s)	699
GsaI	CCCAGC	2 cut(s)	291, 1238
GsuI	CTGGAG	1 cut(s)	484
HaeIII	GGCC	2 cut(s)	460, 909
HapII	CCGG	3 cut(s)	249, 797, 910
HgaI	GACGC	1 cut(s)	1169
HhaI	GCGC	2 cut(s)	662, 743
Hin1I	GRCGYC	1 cut(s)	1161
Hin1II	CATG	6 cut(s)	316, 596, 853, 1195, 1404, 1411
Hin6I	GCGC	2 cut(s)	660, 741
HinP1I	GCGC	2 cut(s)	660, 741
HincII	GTYRAC	1 cut(s)	810
HindII	GTYRAC	1 cut(s)	810
HindIII	AAGCTT	2 cut(s)	124, 758
HpaII	CCGG	3 cut(s)	249, 797, 910
HphI	GGTGA	6 cut(s)	176, 406, 548, 786, 850, 1173
Hpy166II	GTNNAC	5 cut(s)	449, 554, 810, 1159, 1293
Hpy188III	TCNNGA	5 cut(s)	131, 185, 240, 268, 850
Hpy8I	GTNNAC	5 cut(s)	449, 554, 810, 1159, 1293
HpyAV	CCTTC	6 cut(s)	310, 761, 899, 933, 1344, 1364
HpyCH4III	ACNGT	3 cut(s)	952, 1156, 1261
HpyCH4IV	ACGT	1 cut(s)	39
HpyCH4V	TGCA	7 cut(s)	49, 449, 486, 503, 716, 960, 989
HpyF10VI	GCNNNNNNNGC	3 cut(s)	305, 707, 1307
HpyF3I	CTNAG	3 cut(s)	27, 1069, 1466
HpySE526I	ACGT	1 cut(s)	39
Hsp92I	GRCGYC	1 cut(s)	1161
Hsp92II	CATG	6 cut(s)	316, 596, 853, 1195, 1404, 1411
HspAI	GCGC	2 cut(s)	660, 741
Kzo9I	GATC	8 cut(s)	276, 480, 577, 604, 942, 966, 1178, 1253
Lsp1109I	GCAGC	1 cut(s)	685
LweI	GCATC	1 cut(s)	428
MaeI	CTAG	1 cut(s)	131
MaeII	ACGT	1 cut(s)	39
MaeIII	GTNAC	2 cut(s)	792, 869
MalI	GATC	8 cut(s)	278, 482, 579, 606, 944, 968, 1180, 1255
MboI	GATC	8 cut(s)	276, 480, 577, 604, 942, 966, 1178, 1253
MboII	GAAGA	3 cut(s)	682, 1216, 1402
MflI	RGATCY	2 cut(s)	480, 1253
MhlI	GDGCHC	1 cut(s)	451
MlyI	GAGTC	2 cut(s)	671, 1028
MmeI	TCCRAC	4 cut(s)	456, 916, 1340, 1420
MroXI	GAANNNNTTC	1 cut(s)	126
MseI	TTAA	6 cut(s)	212, 261, 756, 876, 1287, 1349
MslI	CAYNNNNRTG	1 cut(s)	876
MspA1I	CMGCKG	1 cut(s)	698
MspI	CCGG	3 cut(s)	249, 797, 910
MspR9I	CCNGG	2 cut(s)	250, 463
Mva1269I	GAATGC	1 cut(s)	618
MvaI	CCWGG	1 cut(s)	463
MvnI	CGCG	1 cut(s)	749
MwoI	GCNNNNNNNGC	3 cut(s)	305, 707, 1307
NciI	CCSGG	1 cut(s)	250
NdeII	GATC	8 cut(s)	276, 480, 577, 604, 942, 966, 1178, 1253
NlaIII	CATG	6 cut(s)	316, 596, 853, 1195, 1404, 1411
NlaIV	GGNNCC	1 cut(s)	651
NmuCI	GTSAC	2 cut(s)	792, 869
PagI	TCATGA	1 cut(s)	849
PctI	GAATGC	1 cut(s)	618
PdmI	GAANNNNTTC	1 cut(s)	126
PfeI	GAWTC	9 cut(s)	62, 134, 243, 469, 779, 841, 928, 1056, 1264
Pfl23II	CGTACG	1 cut(s)	36
PflFI	GACNNNGTC	1 cut(s)	1396
PflMI	CCANNNNNTGG	1 cut(s)	1275
PkrI	GCNGC	1 cut(s)	700
PleI	GAGTC	2 cut(s)	671, 1027
PpsI	GAGTC	2 cut(s)	671, 1027
Ppu21I	YACGTR	1 cut(s)	40
Psp6I	CCWGG	1 cut(s)	461
PspFI	CCCAGC	2 cut(s)	287, 1234
PspGI	CCWGG	1 cut(s)	461
PspLI	CGTACG	1 cut(s)	36
PspN4I	GGNNCC	1 cut(s)	651
PspPI	GGNCC	1 cut(s)	459
PstNI	CAGNNNCTG	1 cut(s)	1022
PsuI	RGATCY	2 cut(s)	480, 1253
PsyI	GACNNNGTC	1 cut(s)	1396
PvuII	CAGCTG	1 cut(s)	698
RsaI	GTAC	2 cut(s)	38, 1100
RsaNI	GTAC	2 cut(s)	37, 1099
RseI	CAYNNNNRTG	1 cut(s)	876
SalI	GTCGAC	1 cut(s)	808
SaqAI	TTAA	6 cut(s)	212, 261, 756, 876, 1287, 1349
SatI	GCNGC	1 cut(s)	699
Sau3AI	GATC	8 cut(s)	276, 480, 577, 604, 942, 966, 1178, 1253
Sau96I	GGNCC	1 cut(s)	459
SchI	GAGTC	2 cut(s)	671, 1028
ScrFI	CCNGG	2 cut(s)	250, 463
SduI	GDGCHC	1 cut(s)	451
SfaNI	GCATC	1 cut(s)	428
SmiMI	CAYNNNNRTG	1 cut(s)	876
SmlI	CTYRAG	3 cut(s)	682, 1144, 1375
SmoI	CTYRAG	3 cut(s)	682, 1144, 1375
SsiI	CCGC	2 cut(s)	162, 254
SspI	AATATT	1 cut(s)	1456
SspMI	CTAG	1 cut(s)	131
StyD4I	CCNGG	2 cut(s)	248, 461
StyI	CCWWGG	5 cut(s)	663, 785, 813, 1051, 1164
TaaI	ACNGT	3 cut(s)	952, 1156, 1261
TaiI	ACGT	1 cut(s)	42
TaqI	TCGA	6 cut(s)	174, 192, 275, 576, 610, 809
TfiI	GAWTC	9 cut(s)	62, 134, 243, 469, 779, 841, 928, 1056, 1264
Tru1I	TTAA	6 cut(s)	212, 261, 756, 876, 1287, 1349
Tru9I	TTAA	6 cut(s)	212, 261, 756, 876, 1287, 1349
TscAI	CASTG	4 cut(s)	456, 1080, 1161, 1230
TseFI	GTSAC	2 cut(s)	792, 869
TseI	GCWGC	1 cut(s)	698
Tsp45I	GTSAC	2 cut(s)	792, 869
TspDTI	ATGAA	3 cut(s)	329, 941, 1396
TspGWI	ACGGA	1 cut(s)	1049
TspRI	CASTG	4 cut(s)	456, 1080, 1161, 1230
Tth111I	GACNNNGTC	1 cut(s)	1396
Van91I	CCANNNNNTGG	1 cut(s)	1275
VneI	GTGCAC	1 cut(s)	447
XapI	RAATTY	1 cut(s)	142
XbaI	TCTAGA	1 cut(s)	130
XcmI	CCANNNNNNNNNTGG	1 cut(s)	820
XmiI	GTMKAC	1 cut(s)	809
XmnI	GAANNNNTTC	1 cut(s)	126
XspI	CTAG	1 cut(s)	131

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.