MD17G1226200.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0016887
Physical Location & Seq
Reverse (-)
27435331 .. 27438624
3294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1226200.v1.1.491

Sequence Viewer

Length: 3015 bp
ATGGCTTTCGTCGAAACCATGTTTTTTCTTCTAATCCAGCATTTGGTCTCGGCATCTAGTAATGCAGTTATTGGTCATCTCCAGAGTCCTCATCACTCTTTGATTGCAGACAAGGCTGCTCTGCTGGAGTTCAAGAAGGCCATTGTATATGATCCGTATTCCAAGCTGGCTAATTGGAAGGAAGCTGCTGATGTCTGCAAGTTCACGGGTGTCAGCTGCAACTTCACGGATGTCAGCTGCAACTTCACGGGTGTCAGCTGCAACACACAGCATTATCGCGTATCAGATCTCATTCTGAAGGATTATGCACTTGCAGGGAGGCTTTCCCCATTCATTTCAAATCTTACAGGCCTCCGTAACCTGGTGCTAGTTGGAAATCACTTCTATGGAACTATCCCTGCTGAAATTGGCTCACTCAGGCGCCTCCATCGTCTACTGCTAGAGGGGAACAACTTTCATGGTTCAGTACCAGATTCCTTAGCCCTTCCTTCAGACCTCACTACAGTTAGTCTATTGCAAAATAATTTAACCGGCGCCATTCCACCTACACTCTTCTCCAACTGCAGTATGTTAGCGGTTTTAGACCTTTCCAACAACCTTCTTTCCGGAAAAATTCCGATAGAGATTGGAAATTGTCCAAAACTATGGAGCCTCAATTTATACAACAATCAGTTCACTGGAGAACTTCCTCTCTTTCTGACCAATAGCTCACTGGTCAACTTAGATGTCGAATATAACCATCTTTCTGGAGAGTTGCCCATCAAGTTTGTACAGAAGTTGCCAAATATTCTCTATCTTCATTTATCTAACAATGATATGGTAAGCCACGATGGCAACACCAATCTTGACCAATTTTTCTCTGCCATCACAAATTGCACTTCTCTAGAGGAGCTTGAGCTGGCTGGTATGGGCCTCGGAGGAATATTGCCCAGTTCCATCGGTGGACTTGGTATCAATTTTACGAATCTTTTTCTGCAAGAAAACCAGATATTTGGATCAATTCCTCCGAATATAGGTAATCTTTCTAAGCTTGTGGTCCTGAATTTGACATCCAATCGTCTGAATGGAACAATCTCAGCAAACATCAGTCAGCTGTCAAATTTGGAACAGCTTTTCTTATCACACAACCTGTTCACCGGCGCAATTCCAGCTGCATTGGGTCAGATGACTCACCTCGGCCTGCTTGATCTCTCTGATAACTCATTCTCCGGTGATATTCCAAGCAGTATAGGAAATTTGGTCAGCCTGAATTATCTGTTCCTCAACAACAACCTCCTTTCCGGATCAATACCTCCAACATTAGGACACTGCATACAACTGTACAAGCTTGATTTATCATACAACAGATTGACAGGCAGCATCCCCCCACAATTATTGGGTTTGAGTGAGATCAGAATTTTTATAAACCTCTCACACAATCAACTCGAAGGACCTATACCGATTGAACTCAGCAAACTGGAAGATGTTCAAGAGATGGATCTTTCATCGAACAACCTAAGTGGAAGTATCTTCCCACAGATATCAAGTTGTATTGCATTGACCCTGATTAACTTTTCACACAATTCTTTGGAGGGGAAACTTCCAGATTCCATAGGTGAGCTGAAGAATCTTGAGTCCCTTGATATTTCAGGAAACCGCTTCTCTGGAAATATTCCACTAAGCCTCAACAACAGCCGAACGCTTACATATCTAAATCTTTCCTTCAATAACTTTGAAGGAATGATTCCCTCTGGTGGCATCTTTGAAGCTGTATCATATACATCTTTCTTGGGCAATCAGCACCTTTGTGGTACTGTTTCCTTCAGACCTGCTTGCCCCCACAAAATGCATCCGTTTCGATCGCACATTTTCTTAATCCTATTCAGCTTAGTCATATTCATTTCAACATCCCTATCAATACTATGCTCTGTGATTGCAATCTGGCACATTCAGGCACTAATCTCAGCTCAGAGGACTGCGACAGCAAGAAAACCAACACAACCAGAATTGGTTCACAGTTTCCCAAGAGTCACATATCGAGATCTGTCGGATGCCACTAACGGATTTGATGATCAGAGACTGATTGGAACTGGCAGCTATGGACGCGTGTACAGGGGAGTTCTTCCAAATGGGACAACAATAGCAGTCAAGGTCTTACAGTTGCAATCTGGAAATTCAACAAAGAGTTTTACCAGAGAGTGCCAGGTCTTGAGGAGAATTAGGCACAGAAACCTTATAAGGATTATAACAGCATGCAGTCTACCGGACTTTAAGGCCATTGTTCTTCCTTATATGGCAAACGGTAGCTTAGAAAGCTGTCTCTATCAACATTCGAAGACAGGTCTGCGTTCAGGTTCTTCTGATTTGAGCCTGATCCAAAGGGTGAACATTTGCAGTGACATAGCTGAAGGGATGGCATATCTACATCACCACTCTCCAGTCAAAGTTATACACTGTGATCTAAAACCGAGCAATGTTCTTCTCAACAATGACATGACAGCTTTAGTTTCCGATTTTGGGATCGCAAGATTGCTGATAGCTGGAGGTGGAAATTCAGCTCTTGAGAACATGGGAAACTCTACTGCAAATATGTTATTCGGATCCATTGGATACATAGCACCAGAATACGGGTATGGTTCTAATGCATCTACAAAAGGTGATGTTTACAGCTTCGGCGTTCTGGTTCTAGAGATGGTGACAAGAAAAAGGCCAACAGATGACATGTTTGTTGGAGGGCTAAACCTGCACAGGTGGGTTAAGAACCATTGTCATGGAAGGGTTGAAAAAGTGGTTGACTTCTCCCTAATGAGAGCTTCTAGAGATCAATCCGCCGAGGTGAGAAAGATGTGGGATGTTGCAATTGGAGAGTTGATAGAGTTGGGAATCCTCTGCACGCAGGAGTCCCCCTCCACTAGGCCGACAATGCTCGATGCTGCGGATGACTTGGATCGACTGAAGAGATATCTCTGTGGGGATACTACTGCAACATTTGCCCCTTCACTCGGAATTTCTTCTTCTACCCTAGACGGTGACTAG

Protein Analysis

1005

Amino Acids

109.61

Weight (kDa)

6.28

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 74 4e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 167 - 225 1.3e-08 Leucine rich repeat
Pkinase PF00069 683 - 968 9.4e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 685 - 969 5.6e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016887)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28380 FvH4_6g28380
malus_domestica MD17G1226200.v1.1
prunus_persica Prupe.3G102400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0132491
rosa_laevigata RLG00000019260
rosa_multiflora Rmu_sc0005538.1_g000005
rosa_roxburghii Rroxscaffold_2G00111910
rosa_rugosa Rorug02G0304800
rosa_samantha Rh2AG357600 Rh2BG364200 Rh2CG341300 Rh2DG380900
rosa_wichuraiana Rw2G029100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1403, 2216, 2225
Acc36I ACCTGC 2 cut(s) 1816, 2730
AccB1I GGYRCC 2 cut(s) 420, 533
AccB7I CCANNNNNTGG 1 cut(s) 43
AccI GTMKAC 2 cut(s) 433, 2239
AccII CGCG 2 cut(s) 279, 2085
AccIII TCCGGA 2 cut(s) 605, 1280
AciI CCGC 4 cut(s) 575, 1636, 2808, 2915
AclWI GGATC 9 cut(s) 146, 1003, 1291, 1485, 2347, 2507, 2574, 2587, 2934
AcsI RAATTY 8 cut(s) 612, 1042, 1099, 1234, 1395, 2152, 2530, 2985
AcuI CTGAAG 6 cut(s) 317, 474, 1622, 1786, 2406, 2954
AcyI GRCGYC 2 cut(s) 421, 534
AfaI GTAC 5 cut(s) 468, 771, 1322, 1792, 2090
AfiI CCNNNNNNNGG 9 cut(s) 43, 361, 1013, 1301, 1733, 2496, 2606, 2892, 2981
AflIII ACRYGT 2 cut(s) 2083, 2700
AjnI CCWGG 2 cut(s) 360, 2181
AleI CACNNNNGTG 1 cut(s) 1785
Alw26I GTCTC 3 cut(s) 52, 2050, 2303
AlwI GGATC 9 cut(s) 146, 1003, 1291, 1485, 2347, 2507, 2574, 2587, 2934
AlwNI CAGNNNCTG 1 cut(s) 2059
Aor13HI TCCGGA 2 cut(s) 605, 1280
AoxI GGCC 7 cut(s) 138, 349, 910, 1177, 2253, 2687, 2894
ApeKI GCWGC 9 cut(s) 116, 185, 216, 237, 258, 1151, 1356, 2073, 2912
ApoI RAATTY 8 cut(s) 612, 1042, 1099, 1234, 1395, 2152, 2530, 2985
Asp700I GAANNNNTTC 3 cut(s) 1464, 1989, 2989
AspLEI GCGC 3 cut(s) 423, 536, 1142
AspS9I GGNCC 3 cut(s) 910, 1036, 1430
AsuHPI GGTGA 9 cut(s) 1126, 1163, 1223, 1607, 2374, 2399, 2648, 2686, 2827
AsuII TTCGAA 1 cut(s) 2312
AvaII GGWCC 2 cut(s) 1036, 1430
BamHI GGATCC 1 cut(s) 2579
BanI GGYRCC 2 cut(s) 420, 533
BarI GAAGNNNNNNTAC 2 cut(s) 1419, 1451
BbsI GAAGAC 1 cut(s) 2321
BbvI GCAGC 9 cut(s) 103, 172, 203, 224, 245, 1138, 1368, 2085, 2899
BccI CCATC 9 cut(s) 435, 747, 767, 824, 872, 944, 1468, 2386, 2665
BcgI CGANNNNNNTGC 2 cut(s) 1817, 1851
BciT130I CCWGG 2 cut(s) 362, 2183
BciVI GTATCC 2 cut(s) 2582, 2947
BclI TGATCA 1 cut(s) 2050
BcoDI GTCTC 3 cut(s) 52, 2050, 2303
BfaI CTAG 9 cut(s) 57, 368, 440, 884, 2666, 2796, 2892, 3002, 3013
BfmI CTRYAG 2 cut(s) 501, 562
BfoI RGCGCY 2 cut(s) 424, 537
BfuAI ACCTGC 2 cut(s) 1816, 2730
BfuI GTATCC 2 cut(s) 2582, 2947
BglI GCCNNNNNGGC 1 cut(s) 831
BglII AGATCT 2 cut(s) 286, 2020
BisI GCNGC 9 cut(s) 117, 186, 217, 238, 259, 1152, 1357, 2074, 2913
BlsI GCNGC 9 cut(s) 118, 187, 218, 239, 260, 1153, 1358, 2075, 2914
Bme1390I CCNGG 2 cut(s) 362, 2183
Bme18I GGWCC 2 cut(s) 1036, 1430
BmgT120I GGNCC 3 cut(s) 910, 1036, 1430
BmiI GGNNCC 4 cut(s) 422, 535, 650, 2581
BmrFI CCNGG 2 cut(s) 362, 2183
BmrI ACTGGG 1 cut(s) 924
BmsI GCATC 7 cut(s) 62, 1368, 1746, 1837, 2020, 2633, 2899
BmuI ACTGGG 1 cut(s) 924
BpiI GAAGAC 1 cut(s) 2321
BplI GAGNNNNNCTC 2 cut(s) 2870, 2902
BpmI CTGGAG 6 cut(s) 65, 146, 699, 768, 2400, 2541
Bpu10I CCTNAGC 1 cut(s) 478
Bpu14I TTCGAA 1 cut(s) 2312
BpuEI CTTGAG 4 cut(s) 914, 1631, 2209, 2561
BsaBI GATNNNNATC 1 cut(s) 1916
BsaHI GRCGYC 2 cut(s) 421, 534
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 3 cut(s) 913, 1174, 2811
BsaWI WCCGGW 4 cut(s) 605, 1208, 1280, 2242
BsaXI ACNNNNNCTCC 2 cut(s) 1190, 1220
Bsc4I CCNNNNNNNGG 9 cut(s) 43, 361, 1013, 1301, 1733, 2496, 2606, 2892, 2981
Bse118I RCCGGY 2 cut(s) 530, 1136
Bse1I ACTGG 6 cut(s) 682, 717, 930, 1461, 2074, 2417
Bse3DI GCAATG 1 cut(s) 2458
Bse8I GATNNNNATC 1 cut(s) 1916
BseAI TCCGGA 2 cut(s) 605, 1280
BseBI CCWGG 2 cut(s) 362, 2183
BseDI CCNNGG 3 cut(s) 913, 1174, 2811
BseGI GGATG 9 cut(s) 235, 1049, 1359, 1828, 1886, 2035, 2397, 2836, 2923
BseJI GATNNNNATC 1 cut(s) 1916
BseLI CCNNNNNNNGG 9 cut(s) 43, 361, 1013, 1301, 1733, 2496, 2606, 2892, 2981
BseMI GCAATG 1 cut(s) 2458
BseMII CTCAG 5 cut(s) 430, 1089, 1462, 1956, 1961
BseNI ACTGG 6 cut(s) 682, 717, 930, 1461, 2074, 2417
BseRI GAGGAG 2 cut(s) 902, 2206
BseXI GCAGC 9 cut(s) 103, 172, 203, 224, 245, 1138, 1368, 2085, 2899
BsgI GTGCAG 2 cut(s) 2708, 2854
Bsh1236I CGCG 2 cut(s) 279, 2085
Bsh1285I CGRYCG 1 cut(s) 1841
BshFI GGCC 7 cut(s) 140, 351, 912, 1179, 2255, 2689, 2896
BshNI GGYRCC 2 cut(s) 420, 533
BsiEI CGRYCG 1 cut(s) 1841
BsiSI CCGG 6 cut(s) 531, 606, 1137, 1209, 1281, 2243
BslFI GGGAC 3 cut(s) 1600, 2125, 2866
BslI CCNNNNNNNGG 9 cut(s) 43, 361, 1013, 1301, 1733, 2496, 2606, 2892, 2981
BsmAI GTCTC 3 cut(s) 52, 2050, 2303
BsmFI GGGAC 3 cut(s) 1600, 2125, 2866
BsnI GGCC 7 cut(s) 140, 351, 912, 1179, 2255, 2689, 2896
Bso31I GGTCTC 1 cut(s) 52
Bsp119I TTCGAA 1 cut(s) 2312
Bsp13I TCCGGA 2 cut(s) 605, 1280
Bsp1407I TGTACA 3 cut(s) 769, 1320, 2088
BspACI CCGC 4 cut(s) 575, 1636, 2808, 2915
BspANI GGCC 7 cut(s) 140, 351, 912, 1179, 2255, 2689, 2896
BspCNI CTCAG 5 cut(s) 429, 1088, 1461, 1955, 1960
BspEI TCCGGA 2 cut(s) 605, 1280
BspFNI CGCG 2 cut(s) 279, 2085
BspLI GGNNCC 4 cut(s) 422, 535, 650, 2581
BspMAI CTGCAG 1 cut(s) 566
BspMI ACCTGC 2 cut(s) 1816, 2730
BspPI GGATC 9 cut(s) 146, 1003, 1291, 1485, 2347, 2507, 2574, 2587, 2934
BspT104I TTCGAA 1 cut(s) 2312
BspT107I GGYRCC 2 cut(s) 420, 533
BspTNI GGTCTC 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 2458
BsrFI RCCGGY 2 cut(s) 530, 1136
BsrGI TGTACA 3 cut(s) 769, 1320, 2088
BsrI ACTGG 6 cut(s) 682, 717, 930, 1461, 2074, 2417
BssAI RCCGGY 2 cut(s) 530, 1136
BssECI CCNNGG 3 cut(s) 913, 1174, 2811
BssNI GRCGYC 2 cut(s) 421, 534
Bst2UI CCWGG 2 cut(s) 362, 2183
Bst4CI ACNGT 8 cut(s) 505, 1320, 1795, 1997, 2139, 2282, 2435, 3008
Bst6I CTCTTC 2 cut(s) 557, 2930
BstACI GRCGYC 2 cut(s) 421, 534
BstAPI GCANNNNNTGC 1 cut(s) 2969
BstAUI TGTACA 3 cut(s) 769, 1320, 2088
BstBI TTCGAA 1 cut(s) 2312
BstC8I GCNNGC 6 cut(s) 168, 900, 1181, 1813, 2233, 2873
BstENI CCTNNNNNAGG 1 cut(s) 2890
BstF5I GGATG 9 cut(s) 235, 1049, 1359, 1828, 1886, 2035, 2397, 2836, 2923
BstFNI CGCG 2 cut(s) 279, 2085
BstH2I RGCGCY 2 cut(s) 424, 537
BstHHI GCGC 3 cut(s) 423, 536, 1142
BstMAI GTCTC 3 cut(s) 52, 2050, 2303
BstMCI CGRYCG 1 cut(s) 1841
BstMWI GCNNNNNNNGC 8 cut(s) 113, 831, 1148, 2082, 2292, 2722, 2902, 2969
BstNI CCWGG 2 cut(s) 362, 2183
BstNSI RCATGY 2 cut(s) 2235, 2704
BstSCI CCNGG 2 cut(s) 360, 2181
BstSFI CTRYAG 2 cut(s) 501, 562
BstUI CGCG 2 cut(s) 279, 2085
BstV1I GCAGC 9 cut(s) 103, 172, 203, 224, 245, 1138, 1368, 2085, 2899
BstV2I GAAGAC 1 cut(s) 2321
BstX2I RGATCY 4 cut(s) 286, 1477, 2020, 2579
BstXI CCANNNNNNTGG 4 cut(s) 645, 746, 992, 2750
BstYI RGATCY 4 cut(s) 286, 1477, 2020, 2579
BsuI GTATCC 2 cut(s) 2582, 2947
BsuRI GGCC 7 cut(s) 140, 351, 912, 1179, 2255, 2689, 2896
BtsCI GGATG 9 cut(s) 235, 1049, 1359, 1828, 1886, 2035, 2397, 2836, 2923
BtsI GCAGTG 2 cut(s) 1306, 2380
BtsIMutI CAGTG 5 cut(s) 675, 710, 1306, 2380, 2431
BveI ACCTGC 2 cut(s) 1816, 2730
Cac8I GCNNGC 6 cut(s) 168, 900, 1181, 1813, 2233, 2873
CaiI CAGNNNCTG 1 cut(s) 2059
CfoI GCGC 3 cut(s) 423, 536, 1142
Cfr10I RCCGGY 2 cut(s) 530, 1136
Cfr13I GGNCC 3 cut(s) 910, 1036, 1430
CseI GACGC 1 cut(s) 2091
CsiI ACCWGGT 1 cut(s) 360
Csp6I GTAC 5 cut(s) 467, 770, 1321, 1791, 2089
CspCI CAANNNNNGTGG 4 cut(s) 1356, 1391, 1480, 1515
CviAII CATG 7 cut(s) 19, 458, 2232, 2473, 2548, 2701, 2750
CviQI GTAC 5 cut(s) 467, 770, 1321, 1791, 2089
DinI GGCGCC 2 cut(s) 422, 535
Eam1104I CTCTTC 2 cut(s) 557, 2930
EarI CTCTTC 2 cut(s) 557, 2930
EciI GGCGGA 1 cut(s) 2797
Eco147I AGGCCT 1 cut(s) 351
Eco31I GGTCTC 1 cut(s) 52
Eco32I GATATC 2 cut(s) 1521, 2942
Eco47I GGWCC 2 cut(s) 1036, 1430
Eco57I CTGAAG 6 cut(s) 317, 474, 1622, 1786, 2406, 2954
EcoNI CCTNNNNNAGG 1 cut(s) 2890
EcoO109I RGGNCCY 1 cut(s) 1430
EcoRII CCWGG 2 cut(s) 360, 2181
EcoRV GATATC 2 cut(s) 1521, 2942
EcoT22I ATGCAT 2 cut(s) 1830, 2626
EgeI GGCGCC 2 cut(s) 422, 535
EheI GGCGCC 2 cut(s) 422, 535
FaeI CATG 7 cut(s) 22, 461, 2235, 2476, 2551, 2704, 2753
FaqI GGGAC 3 cut(s) 1600, 2125, 2866
FatI CATG 7 cut(s) 18, 457, 2231, 2472, 2547, 2700, 2749
FbaI TGATCA 1 cut(s) 2050
FblI GTMKAC 2 cut(s) 433, 2239
Fnu4HI GCNGC 9 cut(s) 117, 186, 217, 238, 259, 1152, 1357, 2074, 2913
FokI GGATG 9 cut(s) 242, 1036, 1346, 1815, 1873, 2042, 2404, 2843, 2930
Fsp4HI GCNGC 9 cut(s) 117, 186, 217, 238, 259, 1152, 1357, 2074, 2913
FspBI CTAG 9 cut(s) 57, 368, 440, 884, 2666, 2796, 2892, 3002, 3013
GlaI GCGC 3 cut(s) 422, 535, 1141
GluI GCNGC 9 cut(s) 117, 186, 217, 238, 259, 1152, 1357, 2074, 2913
GsuI CTGGAG 6 cut(s) 65, 146, 699, 768, 2400, 2541
HaeII RGCGCY 2 cut(s) 424, 537
HaeIII GGCC 7 cut(s) 140, 351, 912, 1179, 2255, 2689, 2896
HapII CCGG 6 cut(s) 531, 606, 1137, 1209, 1281, 2243
HgaI GACGC 1 cut(s) 2091
HhaI GCGC 3 cut(s) 423, 536, 1142
Hin1I GRCGYC 2 cut(s) 421, 534
Hin1II CATG 7 cut(s) 22, 461, 2235, 2476, 2551, 2704, 2753
Hin6I GCGC 3 cut(s) 421, 534, 1140
HinP1I GCGC 3 cut(s) 421, 534, 1140
HincII GTYRAC 2 cut(s) 718, 2773
HindII GTYRAC 2 cut(s) 718, 2773
HindIII AAGCTT 2 cut(s) 1028, 1325
HpaII CCGG 6 cut(s) 531, 606, 1137, 1209, 1281, 2243
HphI GGTGA 9 cut(s) 1126, 1163, 1223, 1607, 2374, 2399, 2648, 2686, 2827
Hpy99I CGWCG 1 cut(s) 14
HpyCH4III ACNGT 8 cut(s) 505, 1320, 1795, 1997, 2139, 2282, 2435, 3008
HpyF10VI GCNNNNNNNGC 8 cut(s) 113, 831, 1148, 2082, 2292, 2722, 2902, 2969
Hsp92I GRCGYC 2 cut(s) 421, 534
Hsp92II CATG 7 cut(s) 22, 461, 2235, 2476, 2551, 2704, 2753
HspAI GCGC 3 cut(s) 421, 534, 1140
KasI GGCGCC 2 cut(s) 420, 533
Kpn2I TCCGGA 2 cut(s) 605, 1280
Ksp22I TGATCA 1 cut(s) 2050
LmnI GCTCC 2 cut(s) 648, 889
Lsp1109I GCAGC 9 cut(s) 103, 172, 203, 224, 245, 1138, 1368, 2085, 2899
LweI GCATC 7 cut(s) 62, 1368, 1746, 1837, 2020, 2633, 2899
MabI ACCWGGT 1 cut(s) 360
MaeI CTAG 9 cut(s) 57, 368, 440, 884, 2666, 2796, 2892, 3002, 3013
MaeIII GTNAC 5 cut(s) 356, 2008, 2375, 2674, 3008
MfeI CAATTG 1 cut(s) 2838
MflI RGATCY 4 cut(s) 286, 1477, 2020, 2579
MluI ACGCGT 1 cut(s) 2083
Mly113I GGCGCC 2 cut(s) 421, 534
MlyI GAGTC 5 cut(s) 94, 1162, 1622, 2016, 2888
MmeI TCCRAC 6 cut(s) 352, 582, 615, 1319, 2007, 2689
Mph1103I ATGCAT 2 cut(s) 1830, 2626
MroI TCCGGA 2 cut(s) 605, 1280
MroXI GAANNNNTTC 3 cut(s) 1464, 1989, 2989
MseI TTAA 5 cut(s) 527, 1548, 1853, 2250, 2736
MslI CAYNNNNRTG 3 cut(s) 384, 1785, 2748
MspA1I CMGCKG 5 cut(s) 216, 237, 258, 1093, 1151
MspI CCGG 6 cut(s) 531, 606, 1137, 1209, 1281, 2243
MspR9I CCNGG 2 cut(s) 362, 2183
MunI CAATTG 1 cut(s) 2838
MvaI CCWGG 2 cut(s) 362, 2183
MvnI CGCG 2 cut(s) 279, 2085
MwoI GCNNNNNNNGC 8 cut(s) 113, 831, 1148, 2082, 2292, 2722, 2902, 2969
NarI GGCGCC 2 cut(s) 421, 534
NlaIII CATG 7 cut(s) 22, 461, 2235, 2476, 2551, 2704, 2753
NlaIV GGNNCC 4 cut(s) 422, 535, 650, 2581
NmeAIII GCCGAG 3 cut(s) 29, 1155, 2836
NmuCI GTSAC 4 cut(s) 2008, 2375, 2674, 3008
NsiI ATGCAT 2 cut(s) 1830, 2626
NspI RCATGY 2 cut(s) 2235, 2704
NspV TTCGAA 1 cut(s) 2312
OliI CACNNNNGTG 1 cut(s) 1785
PaeI GCATGC 1 cut(s) 2235
PceI AGGCCT 1 cut(s) 351
PciI ACATGT 1 cut(s) 2700
PdmI GAANNNNTTC 3 cut(s) 1464, 1989, 2989
PfeI GAWTC 6 cut(s) 473, 964, 1586, 1606, 1723, 2862
PflMI CCANNNNNTGG 1 cut(s) 43
PkrI GCNGC 9 cut(s) 118, 187, 218, 239, 260, 1153, 1358, 2075, 2914
Ple19I CGATCG 1 cut(s) 1841
PleI GAGTC 5 cut(s) 93, 1162, 1621, 2015, 2887
PluTI GGCGCC 2 cut(s) 424, 537
PpsI GAGTC 5 cut(s) 93, 1162, 1621, 2015, 2887
PpuMI RGGWCCY 1 cut(s) 1430
PscI ACATGT 1 cut(s) 2700
PsiI TTATAA 3 cut(s) 1403, 2216, 2225
Psp5II RGGWCCY 1 cut(s) 1430
Psp6I CCWGG 2 cut(s) 360, 2181
PspGI CCWGG 2 cut(s) 360, 2181
PspN4I GGNNCC 4 cut(s) 422, 535, 650, 2581
PspPI GGNCC 3 cut(s) 910, 1036, 1430
PspPPI RGGWCCY 1 cut(s) 1430
PstI CTGCAG 1 cut(s) 566
PstNI CAGNNNCTG 1 cut(s) 2059
PsuI RGATCY 4 cut(s) 286, 1477, 2020, 2579
PvuI CGATCG 1 cut(s) 1841
PvuII CAGCTG 5 cut(s) 216, 237, 258, 1093, 1151
RsaI GTAC 5 cut(s) 468, 771, 1322, 1792, 2090
RsaNI GTAC 5 cut(s) 467, 770, 1321, 1791, 2089
RseI CAYNNNNRTG 3 cut(s) 384, 1785, 2748
SaqAI TTAA 5 cut(s) 527, 1548, 1853, 2250, 2736
SatI GCNGC 9 cut(s) 117, 186, 217, 238, 259, 1152, 1357, 2074, 2913
Sau96I GGNCC 3 cut(s) 910, 1036, 1430
SchI GAGTC 5 cut(s) 94, 1162, 1622, 2016, 2888
ScrFI CCNGG 2 cut(s) 362, 2183
SexAI ACCWGGT 1 cut(s) 360
SfaNI GCATC 7 cut(s) 62, 1368, 1746, 1837, 2020, 2633, 2899
SfcI CTRYAG 2 cut(s) 501, 562
SfoI GGCGCC 2 cut(s) 422, 535
SfuI TTCGAA 1 cut(s) 2312
SgrAI CRCCGGYG 1 cut(s) 1136
SinI GGWCC 2 cut(s) 1036, 1430
SmiMI CAYNNNNRTG 3 cut(s) 384, 1785, 2748
SmlI CTYRAG 4 cut(s) 893, 1610, 2188, 2540
SmoI CTYRAG 4 cut(s) 893, 1610, 2188, 2540
SphI GCATGC 1 cut(s) 2235
SseBI AGGCCT 1 cut(s) 351
SsiI CCGC 4 cut(s) 575, 1636, 2808, 2915
SspDI GGCGCC 2 cut(s) 420, 533
SspI AATATT 3 cut(s) 787, 924, 1651
SspMI CTAG 9 cut(s) 57, 368, 440, 884, 2666, 2796, 2892, 3002, 3013
StuI AGGCCT 1 cut(s) 351
StyD4I CCNGG 2 cut(s) 360, 2181
TaaI ACNGT 8 cut(s) 505, 1320, 1795, 1997, 2139, 2282, 2435, 3008
TaqI TCGA 9 cut(s) 12, 729, 1425, 1487, 1837, 2017, 2312, 2907, 2929
TatI WGTACW 3 cut(s) 769, 1320, 2088
TfiI GAWTC 6 cut(s) 473, 964, 1586, 1606, 1723, 2862
Tru1I TTAA 5 cut(s) 527, 1548, 1853, 2250, 2736
Tru9I TTAA 5 cut(s) 527, 1548, 1853, 2250, 2736
TscAI CASTG 5 cut(s) 682, 717, 1313, 2380, 2438
TseFI GTSAC 4 cut(s) 2008, 2375, 2674, 3008
TseI GCWGC 9 cut(s) 116, 185, 216, 237, 258, 1151, 1356, 2073, 2912
Tsp45I GTSAC 4 cut(s) 2008, 2375, 2674, 3008
TspDTI ATGAA 5 cut(s) 322, 446, 788, 1473, 1867
TspGWI ACGGA 5 cut(s) 144, 242, 344, 1821, 2055
TspRI CASTG 5 cut(s) 682, 717, 1313, 2380, 2438
Van91I CCANNNNNTGG 1 cut(s) 43
VpaK11BI GGWCC 2 cut(s) 1036, 1430
XagI CCTNNNNNAGG 1 cut(s) 2890
XapI RAATTY 8 cut(s) 612, 1042, 1099, 1234, 1395, 2152, 2530, 2985
XbaI TCTAGA 3 cut(s) 883, 2665, 2795
XceI RCATGY 2 cut(s) 2235, 2704
XcmI CCANNNNNNNNNTGG 1 cut(s) 709
XmiI GTMKAC 2 cut(s) 433, 2239
XmnI GAANNNNTTC 3 cut(s) 1464, 1989, 2989
XspI CTAG 9 cut(s) 57, 368, 440, 884, 2666, 2796, 2892, 3002, 3013
Zsp2I ATGCAT 2 cut(s) 1830, 2626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.