Rroxscaffold_2G00111910
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016887
Physical Location & Seq
Reverse (-)
36757819 .. 36763253
5435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00111910.1

Sequence Viewer

Length: 2982 bp
ATGGGTTTTCCGGGTCGGACGGACGGAGGCCGGGGTGGCTTCGATCCGGTCCGGGAGGCGGCGGCGAGCAAGGCAGCAACGCGAGGAGGCTCGGCGTGGGGCGGCGATCGCGGGGAGGCGGGTCGGGGGCGGAGGAGGCGGAAGGTGGTCAACCAACCGGTTATAGGTCATCACCACAATCCTCATCACACTTTGCTTAGAGATAAGGCTGCTCTTCTACAGTTCAAGAAGGCCATTACATATGATCCAAATTCCACTCTAGCTAATTGGAATGAAGCTACTGATGTTTGCAACTTCACCGGTGTTGGGTGCAACAAAAAGCATCATCGTGTAACTATACTCCTTCTGCATAGTCGTAAACTTGTAGGGCAGCTTTCACCTATCATTTCAAATCTAACCCGCCTTCGTTACTTGGAGCTTGTGGGCAATCACTTTTATGGAACTATCCCTCCTGAACTTTCCTTACTCAGACACCTACATCATCTGCGGATTGAGGGTAACAACTTTCGTGGTTCTATACCAGATTCCTTAGTCCTTCCTTCACAACTCACAGTTGTTACTCTTTTGCAAAACAATTTCTCTGGTGCAGTTCCACCTGCACTCTTCTCCAACTGCTCTCTGTTAAGGGTGTTCGACCTTTCCAACAACTTTCTTTCAGGGAAAATTCCAAGAGAAATTGGAAATTATGTTGAGTTCAATCATCTTTCTGGTGAATTGCCCGTCAAACTTGTGGAAAACTTGCCTGATATTCTCTATCTTCACTTATCAAATAATAATATGGTAAGCCATGATGGAAACACCAATCTCGATCCATTCTTCACTGCCCTTGCAAATTGCATTAGTCTAGAGGAGCTTGAACTGGCGAGTATGGGACTTGGAGGAATATTACCTAGTTCCATCGGCGGACTTGGTGTCAACTTTACATACCTGTTGCTGCAAGAAAACAAGATCTTTGGATCAGTTCCTTCAAATATAGGTAATTTATCCAAGCTTGTGGTCCTGAATTTGACCTCCAATCTTATGAATGGAACAATTTCAGCAAACATAAGTCAGTTGTCACACTTGGAACAGCTTTTCTTATCTCACAACTTTTTCACCAGCACGATACCAGAAGCACTTGGCCAGCTTCCTCATCTTGGTCTGCTTGATCTGTCTCATAATTTGTTCTTCGGGGATATCCCAAGCGGTCTAGGAAATTTGGCCATGCTGAATTACCTGTTCCTCAACAACAACCTCCTATCTGGAACCATTCCTCCATCATTAGGACTCTGCACAGAACTGTACAAGCTTGACTTATCATTCAACAGATTGGCAGGGGCCATCCCCCCGGAATTATCAGGTTTGAGTGAGATCAGAATTTTCATAAACCTTTCACACAATCATCTTGAAGGGTCTATACCGATTGAGCTCAGCAAGCTAGAAGATGTTCAAGAGATGGATCTCTCATCAAATAACCTAAGCGGGAGTATCTTTCCACAGATATCAAGCTGTATCGCACTGACGCTCATAAACTTCTCACACAATGCTTTGGAAGGGAGCCTTCCGGATCTCATAGGTGAGCTAAAGAACCTTGTGTCTCTTGATGTTTCAGATAACCACTTGTCAGGAAGGATTCCCCTAAGCCTGAACAAGAGTCTAACACTCACATATCTAAATCTTTCATTTAATGACTTCGAAGGAATGGTTCCTTCTGGTGGCATCTTTGAATCAGTGTCATATACCTCTTTCTTGGGCAACAAACATCTTTGTGGTAAAGTTGCTGCGAAACATGTTTGCCCTCGGACAAAGCAATTGTTTCGCTCTCGCATATTCATAATCATGTTTGTCATAGTCATATTCATTTCAACATCCTTATCAATCATATGCTGTGTGATTGCATTCCGCCGCATTAAGGTACTGATTTCATCCCAGAGGGCTGCAACAGTAAAGAAAACAACACAACCTGAAGTGATTCAGAATTTCCCAAGAGTTACATACCGAGATCTGTCAGAGGCCACCGGTGGATTTGATGATCAGAGACTGATTGGCACGGGGAGCTATGGACGTGTCTACAGGGGAGTCCTTCCTGATGGGACAACCATAGCAGTCAAGGTGTTACATTTACAATCTGGAAATTCAACAAAGAGTTTTAACAGAGAGTGCCAAGTCTTAAGGAGAATTAGGCACAGGAACCTTATAAGGATCATAACAGCTTGCAGCCTACCTGATTTCAAGGCTATTGTTCTTCCTTATATGACAAATGGTAGCTTGGATAGCCGTCTCTATCCACATTCACAAGCCGGTCTAGGTTCAGGCTCTTCTGATCTGAGTCTCATTCAGAGGGTCAATATTTGTAGTGACATCGCGGAAGGGATGGCCTATCTTCATCATTACTCTCCGGTCAGAGTTATACACTGTGACCTGAAACCAAGCAATGTTCTTCTCAACGATGGCATGACTGCTTTAGTTTCTGATTTTGGGATAGCAAGATTAGTGATAGCCGGAGGAGGAGTGGGAAATGCAGGTCTTGAGAACATGGGAAACTCTACTGCAAGTTTATTATGTGGATCTATTGGATACATAGCACCAGAGTATGGGTTTGGATCGAACACATCTACAAAAGGCGATGTTTACAGCTTCGGTGTTCTTGTTCTCGAGATGGTGACAAGAAAAAGGCCGACAGATGACATGTTTGTTGGGGGACTAAGCCTGCACAGCTGGGTAAAGAACCATTATCATGGAAGGGTTGACAAAGTGGTCGACTCTTCCTTGGTGAGAGCTTCTAGAGATCAGCCACCTGAGATGAAAAAAATGTGGGATGTTGCCATAGAAGAAATGATAGAGTTGGGTATTCTCTGCACTCAGGAATCCCCTTCCACTAGGCCTACAATGCTCGATGCTGCAGATGATTTGGATCGGCTGAAGAGATATCTTGGTGGGGATACTACTGCAACATTTGCCTCTTCATTAGGAATTTCTTCGTCTACCCTAGACGACGACTAG

Protein Analysis

993

Amino Acids

108.33

Weight (kDa)

7.65

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 67 - 105 2.4e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 333 - 577 1.7e-08 Leucine-rich repeat region
Pkinase PF00069 670 - 960 4.2e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 672 - 960 6.7e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016887)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28380 FvH4_6g28380
malus_domestica MD17G1226200.v1.1
prunus_persica Prupe.3G102400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0132491
rosa_laevigata RLG00000019260
rosa_multiflora Rmu_sc0005538.1_g000005
rosa_roxburghii Rroxscaffold_2G00111910
rosa_rugosa Rorug02G0304800
rosa_samantha Rh2AG357600 Rh2BG364200 Rh2CG341300 Rh2DG380900
rosa_wichuraiana Rw2G029100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2177
AarI CACCTGC 1 cut(s) 604
AasI GACNNNNNNGTC 1 cut(s) 2735
Acc36I ACCTGC 2 cut(s) 604, 2492
AccB7I CCANNNNNTGG 1 cut(s) 2573
AccI GTMKAC 3 cut(s) 2049, 2739, 2963
AccII CGCG 3 cut(s) 82, 111, 2345
AccIII TCCGGA 1 cut(s) 1543
AcoI YGGCCR 2 cut(s) 1120, 1200
AcsI RAATTY 8 cut(s) 250, 663, 1003, 1195, 1356, 1957, 2113, 2952
AcuI CTGAAG 2 cut(s) 1965, 2921
AfaI GTAC 2 cut(s) 1283, 1896
AfiI CCNNNNNNNGG 8 cut(s) 58, 164, 306, 1136, 1262, 1694, 1891, 2573
AflII CTTAAG 1 cut(s) 2149
AflIII ACRYGT 3 cut(s) 1768, 2044, 2667
AgeI ACCGGT 3 cut(s) 157, 299, 1997
AhdI GACNNNNNGTC 1 cut(s) 911
AjiI CACGTC 1 cut(s) 2045
AloI GAACNNNNNNTCC 4 cut(s) 433, 465, 1237, 1269
Alw21I GWGCWC 1 cut(s) 1410
Alw26I GTCTC 5 cut(s) 1158, 1581, 2011, 2264, 2315
AlwNI CAGNNNCTG 1 cut(s) 2020
Ama87I CYCGRG 1 cut(s) 2633
Aor13HI TCCGGA 1 cut(s) 1543
AoxI GGCC 9 cut(s) 28, 231, 1120, 1200, 1317, 1992, 2355, 2654, 2861
ApeKI GCWGC 8 cut(s) 74, 209, 370, 934, 1760, 1916, 2196, 2879
ApoI RAATTY 8 cut(s) 250, 663, 1003, 1195, 1356, 1957, 2113, 2952
AsiGI ACCGGT 3 cut(s) 157, 299, 1997
AsiSI GCGATCGC 1 cut(s) 109
Asp700I GAANNNNTTC 4 cut(s) 1033, 1425, 1950, 2956
AspS9I GGNCC 3 cut(s) 49, 997, 1317
AsuC2I CCSGG 4 cut(s) 12, 32, 53, 1328
AsuHPI GGTGA 8 cut(s) 164, 289, 369, 722, 1087, 1568, 2653, 2764
AsuII TTCGAA 1 cut(s) 1674
AvaI CYCGRG 1 cut(s) 2633
AvaII GGWCC 2 cut(s) 49, 997
BalI TGGCCA 2 cut(s) 1122, 1202
BanII GRGCYC 1 cut(s) 1410
BarI GAAGNNNNNNTAC 2 cut(s) 1380, 1412
Bbv12I GWGCWC 1 cut(s) 1410
BbvI GCAGC 8 cut(s) 86, 196, 382, 921, 1747, 1903, 2208, 2866
BccI CCATC 9 cut(s) 785, 905, 1264, 1328, 1429, 2063, 2347, 2423, 2632
BceAI ACGGC 1 cut(s) 2241
BcgI CGANNNNNNTGC 2 cut(s) 1778, 1812
BciVI GTATCC 2 cut(s) 2549, 2914
BclI TGATCA 1 cut(s) 2011
BcnI CCSGG 4 cut(s) 12, 32, 53, 1328
BcoDI GTCTC 5 cut(s) 1158, 1581, 2011, 2264, 2315
BfmI CTRYAG 3 cut(s) 218, 2050, 2880
BfrI CTTAAG 1 cut(s) 2149
BfuAI ACCTGC 2 cut(s) 604, 2492
BfuI GTATCC 2 cut(s) 2549, 2914
BglI GCCNNNNNGGC 1 cut(s) 36
BglII AGATCT 2 cut(s) 948, 1981
BlpI GCTNAGC 1 cut(s) 1409
Bme1390I CCNGG 4 cut(s) 12, 32, 53, 1328
Bme18I GGWCC 2 cut(s) 49, 997
BmeRI GACNNNNNGTC 1 cut(s) 911
BmeT110I CYCGRG 1 cut(s) 2633
BmgBI CACGTC 1 cut(s) 2045
BmgT120I GGNCC 3 cut(s) 49, 997, 1317
BmiI GGNNCC 5 cut(s) 1246, 1318, 1538, 1686, 2171
BmrFI CCNGG 4 cut(s) 12, 32, 53, 1328
BmsI GCATC 3 cut(s) 331, 1707, 2866
Bpu10I CCTNAGC 2 cut(s) 1457, 1619
Bpu1102I GCTNAGC 1 cut(s) 1409
Bpu14I TTCGAA 1 cut(s) 1674
BpuEI CTTGAG 1 cut(s) 2528
BpuMI CCSGG 4 cut(s) 12, 32, 53, 1328
BsaBI GATNNNNATC 1 cut(s) 2892
BsaJI CCNNGG 4 cut(s) 31, 1326, 1778, 2748
BsaWI WCCGGW 6 cut(s) 46, 157, 299, 1543, 1997, 2377
BsaXI ACNNNNNCTCC 6 cut(s) 433, 463, 1237, 1267, 2475, 2505
Bsc4I CCNNNNNNNGG 8 cut(s) 58, 164, 306, 1136, 1262, 1694, 1891, 2573
Bse118I RCCGGY 4 cut(s) 157, 299, 1997, 2279
Bse1I ACTGG 1 cut(s) 864
Bse3DI GCAATG 1 cut(s) 2419
Bse8I GATNNNNATC 1 cut(s) 2892
BseAI TCCGGA 1 cut(s) 1543
BseDI CCNNGG 4 cut(s) 31, 1326, 1778, 2748
BseGI GGATG 5 cut(s) 1320, 1847, 1904, 2358, 2803
BseJI GATNNNNATC 1 cut(s) 2892
BseLI CCNNNNNNNGG 8 cut(s) 58, 164, 306, 1136, 1262, 1694, 1891, 2573
BseMI GCAATG 1 cut(s) 2419
BseMII CTCAG 5 cut(s) 481, 1423, 2297, 2769, 2855
BseNI ACTGG 1 cut(s) 864
BseRI GAGGAG 5 cut(s) 99, 148, 863, 2499, 2502
BseXI GCAGC 8 cut(s) 86, 196, 382, 921, 1747, 1903, 2208, 2866
BseYI CCCAGC 1 cut(s) 2697
BsgI GTGCAG 5 cut(s) 582, 606, 1255, 2675, 2821
Bsh1236I CGCG 3 cut(s) 82, 111, 2345
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 9 cut(s) 30, 233, 1122, 1202, 1319, 1994, 2357, 2656, 2863
BshTI ACCGGT 3 cut(s) 157, 299, 1997
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 1410
BsiHKCI CYCGRG 1 cut(s) 2633
BslFI GGGAC 3 cut(s) 885, 2086, 2694
BslI CCNNNNNNNGG 8 cut(s) 58, 164, 306, 1136, 1262, 1694, 1891, 2573
BsmAI GTCTC 5 cut(s) 1158, 1581, 2011, 2264, 2315
BsmBI CGTCTC 1 cut(s) 2264
BsmFI GGGAC 3 cut(s) 885, 2086, 2694
BsmI GAATGC 1 cut(s) 1877
BsnI GGCC 9 cut(s) 30, 233, 1122, 1202, 1319, 1994, 2357, 2656, 2863
BsoBI CYCGRG 1 cut(s) 2633
Bsp119I TTCGAA 1 cut(s) 1674
Bsp1286I GDGCHC 1 cut(s) 1410
Bsp13I TCCGGA 1 cut(s) 1543
Bsp1407I TGTACA 1 cut(s) 1281
Bsp1720I GCTNAGC 1 cut(s) 1409
BspANI GGCC 9 cut(s) 30, 233, 1122, 1202, 1319, 1994, 2357, 2656, 2863
BspCNI CTCAG 5 cut(s) 480, 1422, 2298, 2770, 2854
BspEI TCCGGA 1 cut(s) 1543
BspFNI CGCG 3 cut(s) 82, 111, 2345
BspLI GGNNCC 5 cut(s) 1246, 1318, 1538, 1686, 2171
BspMAI CTGCAG 1 cut(s) 2884
BspMI ACCTGC 2 cut(s) 604, 2492
BspQI GCTCTTC 2 cut(s) 219, 2302
BspT104I TTCGAA 1 cut(s) 1674
BspTI CTTAAG 1 cut(s) 2149
BsrDI GCAATG 1 cut(s) 2419
BsrFI RCCGGY 4 cut(s) 157, 299, 1997, 2279
BsrGI TGTACA 1 cut(s) 1281
BsrI ACTGG 1 cut(s) 864
BssAI RCCGGY 4 cut(s) 157, 299, 1997, 2279
BssECI CCNNGG 4 cut(s) 31, 1326, 1778, 2748
BssT1I CCWWGG 1 cut(s) 2748
Bst4CI ACNGT 5 cut(s) 222, 553, 1281, 1924, 2396
Bst6I CTCTTC 6 cut(s) 219, 608, 2302, 2749, 2897, 2947
BstAFI CTTAAG 1 cut(s) 2149
BstAPI GCANNNNNTGC 1 cut(s) 2936
BstAUI TGTACA 1 cut(s) 1281
BstBI TTCGAA 1 cut(s) 1674
BstC8I GCNNGC 5 cut(s) 67, 1124, 1415, 2194, 2690
BstF5I GGATG 5 cut(s) 1320, 1847, 1904, 2358, 2803
BstFNI CGCG 3 cut(s) 82, 111, 2345
BstMAI GTCTC 5 cut(s) 1158, 1581, 2011, 2264, 2315
BstMCI CGRYCG 1 cut(s) 109
BstNSI RCATGY 2 cut(s) 1772, 2671
BstSCI CCNGG 4 cut(s) 10, 30, 51, 1326
BstSFI CTRYAG 3 cut(s) 218, 2050, 2880
BstUI CGCG 3 cut(s) 82, 111, 2345
BstV1I GCAGC 8 cut(s) 86, 196, 382, 921, 1747, 1903, 2208, 2866
BstX2I RGATCY 5 cut(s) 948, 1438, 1546, 1981, 2546
BstXI CCANNNNNNTGG 2 cut(s) 994, 2717
BstYI RGATCY 5 cut(s) 948, 1438, 1546, 1981, 2546
BsuI GTATCC 2 cut(s) 2549, 2914
BsuRI GGCC 9 cut(s) 30, 233, 1122, 1202, 1319, 1994, 2357, 2656, 2863
BtgZI GCGATG 2 cut(s) 2326, 2619
BtrI CACGTC 1 cut(s) 2045
BtsCI GGATG 5 cut(s) 1320, 1847, 1904, 2358, 2803
BtsI GCAGTG 1 cut(s) 819
BtsIMutI CAGTG 4 cut(s) 819, 1496, 1716, 2392
BveI ACCTGC 2 cut(s) 604, 2492
Cac8I GCNNGC 5 cut(s) 67, 1124, 1415, 2194, 2690
CaiI CAGNNNCTG 1 cut(s) 2020
Cfr10I RCCGGY 4 cut(s) 157, 299, 1997, 2279
Cfr13I GGNCC 3 cut(s) 49, 997, 1317
CpoI CGGWCCG 1 cut(s) 49
CseI GACGC 1 cut(s) 1510
Csp6I GTAC 2 cut(s) 1282, 1895
CspAI ACCGGT 3 cut(s) 157, 299, 1997
CspCI CAANNNNNGTGG 2 cut(s) 490, 525
CspI CGGWCCG 1 cut(s) 49
CviAII CATG 8 cut(s) 788, 1204, 1769, 1819, 2434, 2515, 2668, 2717
CviQI GTAC 2 cut(s) 1282, 1895
DrdI GACNNNNNNGTC 1 cut(s) 2735
DriI GACNNNNNGTC 1 cut(s) 911
DseDI GACNNNNNNGTC 1 cut(s) 2735
EaeI YGGCCR 2 cut(s) 1120, 1200
Eam1104I CTCTTC 6 cut(s) 219, 608, 2302, 2749, 2897, 2947
Eam1105I GACNNNNNGTC 1 cut(s) 911
EarI CTCTTC 6 cut(s) 219, 608, 2302, 2749, 2897, 2947
EciI GGCGGA 4 cut(s) 145, 154, 918, 1871
Ecl136II GAGCTC 1 cut(s) 1408
Eco130I CCWWGG 1 cut(s) 2748
Eco147I AGGCCT 1 cut(s) 2863
Eco24I GRGCYC 1 cut(s) 1410
Eco32I GATATC 3 cut(s) 1177, 1482, 2909
Eco47I GGWCC 2 cut(s) 49, 997
Eco53kI GAGCTC 1 cut(s) 1408
Eco57I CTGAAG 2 cut(s) 1965, 2921
Eco88I CYCGRG 1 cut(s) 2633
EcoICRI GAGCTC 1 cut(s) 1408
EcoRV GATATC 3 cut(s) 1177, 1482, 2909
EcoT14I CCWWGG 1 cut(s) 2748
EcoT38I GRGCYC 1 cut(s) 1410
ErhI CCWWGG 1 cut(s) 2748
Esp3I CGTCTC 1 cut(s) 2264
FaeI CATG 8 cut(s) 791, 1207, 1772, 1822, 2437, 2518, 2671, 2720
FalI AAGNNNNNCTT 4 cut(s) 1277, 1309, 1524, 1556
FaqI GGGAC 3 cut(s) 885, 2086, 2694
FatI CATG 8 cut(s) 787, 1203, 1768, 1818, 2433, 2514, 2667, 2716
FauI CCCGC 4 cut(s) 104, 112, 407, 1454
FauNDI CATATG 2 cut(s) 241, 1862
FbaI TGATCA 1 cut(s) 2011
FblI GTMKAC 3 cut(s) 2049, 2739, 2963
FokI GGATG 5 cut(s) 1307, 1834, 1891, 2365, 2810
FriOI GRGCYC 1 cut(s) 1410
GsaI CCCAGC 1 cut(s) 2701
HaeIII GGCC 9 cut(s) 30, 233, 1122, 1202, 1319, 1994, 2357, 2656, 2863
HgaI GACGC 1 cut(s) 1510
Hin1II CATG 8 cut(s) 791, 1207, 1772, 1822, 2437, 2518, 2671, 2720
HincII GTYRAC 4 cut(s) 151, 916, 2728, 2740
HindII GTYRAC 4 cut(s) 151, 916, 2728, 2740
HindIII AAGCTT 2 cut(s) 989, 1286
HphI GGTGA 8 cut(s) 164, 289, 369, 722, 1087, 1568, 2653, 2764
Hpy166II GTNNAC 8 cut(s) 151, 359, 916, 2050, 2611, 2728, 2740, 2964
Hpy8I GTNNAC 8 cut(s) 151, 359, 916, 2050, 2611, 2728, 2740, 2964
Hpy99I CGWCG 1 cut(s) 2978
HpyCH4III ACNGT 5 cut(s) 222, 553, 1281, 1924, 2396
HpyCH4IV ACGT 1 cut(s) 2044
HpySE526I ACGT 1 cut(s) 2044
Hsp92II CATG 8 cut(s) 791, 1207, 1772, 1822, 2437, 2518, 2671, 2720
Kpn2I TCCGGA 1 cut(s) 1543
Ksp22I TGATCA 1 cut(s) 2011
LguI GCTCTTC 2 cut(s) 219, 2302
LmnI GCTCC 4 cut(s) 415, 850, 1536, 2034
Lsp1109I GCAGC 8 cut(s) 86, 196, 382, 921, 1747, 1903, 2208, 2866
LweI GCATC 3 cut(s) 331, 1707, 2866
MaeII ACGT 1 cut(s) 2044
MfeI CAATTG 1 cut(s) 1790
MflI RGATCY 5 cut(s) 948, 1438, 1546, 1981, 2546
MhlI GDGCHC 1 cut(s) 1410
MlsI TGGCCA 2 cut(s) 1122, 1202
MluNI TGGCCA 2 cut(s) 1122, 1202
MlyI GAGTC 5 cut(s) 1260, 1642, 2067, 2317, 2735
MmeI TCCRAC 2 cut(s) 633, 666
Mox20I TGGCCA 2 cut(s) 1122, 1202
MroI TCCGGA 1 cut(s) 1543
MroXI GAANNNNTTC 4 cut(s) 1033, 1425, 1950, 2956
MscI TGGCCA 2 cut(s) 1122, 1202
MseI TTAA 5 cut(s) 623, 1665, 1890, 2130, 2150
MslI CAYNNNNRTG 5 cut(s) 327, 435, 1746, 1817, 2715
Msp20I TGGCCA 2 cut(s) 1122, 1202
MspA1I CMGCKG 1 cut(s) 2697
MspCI CTTAAG 1 cut(s) 2149
MspR9I CCNGG 4 cut(s) 12, 32, 53, 1328
MunI CAATTG 1 cut(s) 1790
Mva1269I GAATGC 1 cut(s) 1877
MvnI CGCG 3 cut(s) 82, 111, 2345
NciI CCSGG 4 cut(s) 12, 32, 53, 1328
NdeI CATATG 2 cut(s) 241, 1862
NlaIII CATG 8 cut(s) 791, 1207, 1772, 1822, 2437, 2518, 2671, 2720
NlaIV GGNNCC 5 cut(s) 1246, 1318, 1538, 1686, 2171
NmeAIII GCCGAG 1 cut(s) 71
NmuCI GTSAC 4 cut(s) 1056, 2336, 2396, 2641
NspI RCATGY 2 cut(s) 1772, 2671
NspV TTCGAA 1 cut(s) 1674
PaeR7I CTCGAG 1 cut(s) 2633
PaqCI CACCTGC 1 cut(s) 604
PceI AGGCCT 1 cut(s) 2863
PciI ACATGT 2 cut(s) 1768, 2667
PciSI GCTCTTC 2 cut(s) 219, 2302
PctI GAATGC 1 cut(s) 1877
PdmI GAANNNNTTC 4 cut(s) 1033, 1425, 1950, 2956
PfeI GAWTC 5 cut(s) 524, 1612, 1706, 1951, 2846
PflMI CCANNNNNTGG 1 cut(s) 2573
PfoI TCCNGGA 1 cut(s) 51
PinAI ACCGGT 3 cut(s) 157, 299, 1997
Ple19I CGATCG 1 cut(s) 109
PleI GAGTC 5 cut(s) 1260, 1641, 2066, 2316, 2735
PpsI GAGTC 5 cut(s) 1260, 1641, 2066, 2316, 2735
PscI ACATGT 2 cut(s) 1768, 2667
PsiI TTATAA 1 cut(s) 2177
Psp124BI GAGCTC 1 cut(s) 1410
PspFI CCCAGC 1 cut(s) 2697
PspN4I GGNNCC 5 cut(s) 1246, 1318, 1538, 1686, 2171
PspPI GGNCC 3 cut(s) 49, 997, 1317
PsrI GAACNNNNNNTAC 2 cut(s) 447, 479
PstI CTGCAG 1 cut(s) 2884
PstNI CAGNNNCTG 1 cut(s) 2020
PsuI RGATCY 5 cut(s) 948, 1438, 1546, 1981, 2546
PvuI CGATCG 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 2697
RgaI GCGATCGC 1 cut(s) 109
RsaI GTAC 2 cut(s) 1283, 1896
RsaNI GTAC 2 cut(s) 1282, 1895
RseI CAYNNNNRTG 5 cut(s) 327, 435, 1746, 1817, 2715
Rsr2I CGGWCCG 1 cut(s) 49
RsrII CGGWCCG 1 cut(s) 49
SacI GAGCTC 1 cut(s) 1410
SalI GTCGAC 1 cut(s) 2738
SapI GCTCTTC 2 cut(s) 219, 2302
SaqAI TTAA 5 cut(s) 623, 1665, 1890, 2130, 2150
Sau96I GGNCC 3 cut(s) 49, 997, 1317
SchI GAGTC 5 cut(s) 1260, 1642, 2067, 2317, 2735
ScrFI CCNGG 4 cut(s) 12, 32, 53, 1328
SduI GDGCHC 1 cut(s) 1410
SfaAI GCGATCGC 1 cut(s) 109
SfaNI GCATC 3 cut(s) 331, 1707, 2866
SfcI CTRYAG 3 cut(s) 218, 2050, 2880
Sfr274I CTCGAG 1 cut(s) 2633
SfuI TTCGAA 1 cut(s) 1674
SgfI GCGATCGC 1 cut(s) 109
SgrAI CRCCGGYG 2 cut(s) 299, 1997
SinI GGWCC 2 cut(s) 49, 997
SlaI CTCGAG 1 cut(s) 2633
SmiMI CAYNNNNRTG 5 cut(s) 327, 435, 1746, 1817, 2715
SmlI CTYRAG 3 cut(s) 2149, 2507, 2633
SmoI CTYRAG 3 cut(s) 2149, 2507, 2633
SseBI AGGCCT 1 cut(s) 2863
SspI AATATT 2 cut(s) 885, 2329
SstI GAGCTC 1 cut(s) 1410
StuI AGGCCT 1 cut(s) 2863
StyD4I CCNGG 4 cut(s) 10, 30, 51, 1326
StyI CCWWGG 1 cut(s) 2748
TaaI ACNGT 5 cut(s) 222, 553, 1281, 1924, 2396
TaiI ACGT 1 cut(s) 2047
TaqI TCGA 8 cut(s) 42, 633, 807, 1674, 2585, 2634, 2739, 2874
TatI WGTACW 1 cut(s) 1281
TauI GCSGC 4 cut(s) 62, 65, 105, 1887
TfiI GAWTC 5 cut(s) 524, 1612, 1706, 1951, 2846
Tru1I TTAA 5 cut(s) 623, 1665, 1890, 2130, 2150
Tru9I TTAA 5 cut(s) 623, 1665, 1890, 2130, 2150
TscAI CASTG 4 cut(s) 826, 1503, 1716, 2399
TseFI GTSAC 4 cut(s) 1056, 2336, 2396, 2641
TseI GCWGC 8 cut(s) 74, 209, 370, 934, 1760, 1916, 2196, 2879
Tsp45I GTSAC 4 cut(s) 1056, 2336, 2396, 2641
TspGWI ACGGA 2 cut(s) 35, 39
TspRI CASTG 4 cut(s) 826, 1503, 1716, 2399
Van91I CCANNNNNTGG 1 cut(s) 2573
Vha464I CTTAAG 1 cut(s) 2149
VpaK11BI GGWCC 2 cut(s) 49, 997
XapI RAATTY 8 cut(s) 250, 663, 1003, 1195, 1356, 1957, 2113, 2952
XbaI TCTAGA 2 cut(s) 844, 2762
XceI RCATGY 2 cut(s) 1772, 2671
XhoI CTCGAG 1 cut(s) 2633
XmiI GTMKAC 3 cut(s) 2049, 2739, 2963
XmnI GAANNNNTTC 4 cut(s) 1033, 1425, 1950, 2956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.