RchiOBHm_Chr2g0132491
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016887
Physical Location & Seq
Forward (+)
49047411 .. 49050556
3146 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50375

Sequence Viewer

Length: 2979 bp
ATGGTCTTCATCAAAACCATTTTCTTTCTTCTATTACAGCATTTTGTTGTTACATCTTGTAATGCAGTTATAGGTCATCACCACAATCCTCATCACTCTTTGCTTAGAGATAAGGCTGCTCTTCTACAGTTCAAGAAGGCCATTACATATGATCCAAATTCCACTCTAGCTAATTGGAATGAAGCTACTGATGTTTGCAACTACACCGGTGTTGGGTGCAACAAAAAGCATCATCGTGTAACTATACTCCTTCTGCATAATCGTAAACTTGTAGGGCAGCTTTCACCTATCATTTCAAATCTAACCCGCCTTCGTTACTTGGAGCTTGTAGGCAATCACTTTTATGGAACTATCCCTCCTGAACTTTCCTTACTCAGACACCTACATCATCTACGGATTGAGGGGAACAACTTTCGTGGTTCTATACCAGATTCCTTAGTCCTTCCTTCACAACTCACAGTTGTTACTCTTTTGCAAAACAATTTCTCTGGTGCAGTTCCACCTGCTCTCTTCTCCAACTGCTCTCTGTTAAGGGTGTTCGACCTTTCCAACAACTTTCTTTCAGGGAAAATTCCAAGAGAAATTGGAAATTGTCCAAACCTATGGACCCTCAATTTATACAACAATCAATTCACTGGAGAAATTCCTCTCTCTTTGACTAATACCTCACTGGTCAATTTAGATGTTGAGTTCAATCATCTTTCAGGTGAATTGCCCGTCAAACTTGTGGAAAACTTGCCTGATATTCTCTATCTTCACTTATCAAATAATAATATGGTAAGCCATGATGGAAACACCAATCTCGATCCATTCTTCACTGCCCTTGCAAATTGCATTAGTCTAGAGGAGCTTGAACTGGCGAGTATGGGACTTGGAGGAATATTACCTAGTTCCATTGGCGGACTTGGTGTCAACTTTACATACCTGTTGCTGCAAGAAAACAAGATCATTGGATCAGTTCCTTCAAATATAGGTAATTTATCCAAGCTTGTGGTCCTGAATTTGACCTCCAATCTTATGAATGGAACAATTTCAGCAAACATAAGTCAGTTGTCACACTTGGAACAGCTTTTCTTATCTCACAACTTTTTCACCAGCACGATACCAGAAGCACTTGGCCATCTTCCTCATCTTGGTCTGCTTGATCTGTCTCATAATTCGTTCTTCGGGGATATCCCAAGCGGTCTAGGAAATTTGGCCATGCTGAATTATCTGTTCCTCAACAACAACCTCCTATCTGGAACCATTCCTCCATCATTAGGACGCTGCACAGAACTGTACAAGCTTGACTTATCATTCAACAGATTGGCAGGGGCCATCCCCCCGGAATTATCAGGTTTGAATGAGATCAGAATTTTCATAAACCTCTCACACAATCACCTTGAAGGGTCCATACCGATTGAGCTCAGCAAGCTAGAAGATGTTCAAGAGATGGATCTCTCATCAAATAACCTAAGCGGGAGTATCTTTCCACAGATATCAAGCTGTATCGCACTGACGCTCATAAACTTCTCACACAATGCTTTGGAAGGGAGCCTTCCGGATTTCATAGGTGAGCTAAAGAACCTTGTGTCTCTTGATGTTTCAGATAACCACTTGTCAGGAAGGATTCCCCTAAGCCTCAACAAGAGTCTAACACTCACATATCTAAATCTTTCATTTAATGACTTCAAAGGAATGGTTCCTTCTGGTGGCATCTTTGAATCAGTGTCATATACCTCTTTCTTAGGCAATAAACATCTTTGTGGTAAAGTTGCTGCGAAACATGTTTGCCCTCGGACAAAGCATTTGTTTCGCTCCCGCATATTCATAATCATGTTTGTCATAGTCATATTCATTTCAACATCCTTATCAATCATATGCTGTGTGATTGCATTCCGCCGCATTAAGGTGCTGATTTCATCCCAGAGGGCTGCAACCGTAAAGAAACCAACACAACCTGAAGTGATTCAGAATTTCCCAAGAGTTACATACCGAGATCTGTCAGAGGCCACCGGTGGATTTGATGATCAGAGACTGATTGGCACGGGGAGCTATGGACGTGTCTACAGGGGAGTCCTTCCAGATGGGACAACCATAGCAGTCAAGGTGTTACATTTACAATCTGGAAATTCAACAAAAAGTTTTAACAGAGAATGCCAAGTCTTAAGGAGAATTAGGCACAGGAACCTTATAAGGATCATAACAGCTTGCAGCCTACCTGATTTCAAGGCTATTGTTCTTCCTTATATGGCAAATGGTAGCTTGGATAGCCGTCTCTATCCACATTCACAAGCCGGTCTAGGTTCAGGCTCTTCTGATCTGAGTCTCATTCAGAGGGTCAATATTTGTAGCGACATCGCGGAAGGGATGGCCTATCTTCATCATTACTCTCCGGTCAGAGTTATACACTGTGACCTGAAACCAAGCAATGTTCTTCTCAACGATGGCATGACTGCTTTAGTTTCTGATTTTGGGATTGCAAGATTAGTGATAGCCGGAGGAGGAGTGGGAAATGCAGGTCTTGAGAACATGGGAAACTCTACTGCAAGTTTATTATGTGGATCTATTGGATACATAGCACCAGAGTATGGGTTTGGATCGAACACATCTACAAAAGGCGATGTTTACAGCTTCGGTGTTCTTGTTCTCGAGATGGTGACAAGAAAAAGGCCGACAGATGACATGTTTGTTGGGGGACTAAGCCTGCACAGCTGGGTAAAGAACCATTATCATGGAAGGGTTGACAAAGTGCTCGACTCTTCCTTGGTGAGAGCTTCTAGAGATCAGCCACCTGAGATGAAAAAAATGTGGGATGTTGCCATAGGAGAAATGATAGAGTTGGGTATTCTCTGCACTCAGGAGTCCCCTTCCACTAGGCCTACAATGCTCGATGCTGCAGATGATTTGGATCGGCTGAAGAGATATCTTGGTGGGGATACTACTGCAACATTTGCCTCTTCATTAGGAATTTCTTCGTCTACCCTAGATGACGACTAG

Protein Analysis

992

Amino Acids

108.73

Weight (kDa)

6.96

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 74 9.6e-12 Leucine rich repeat N-terminal domain
LRR_8 PF13855 152 - 211 6.5e-08 Leucine rich repeat
LRR_8 PF13855 329 - 387 7.5e-07 Leucine rich repeat
LRR_14 PF23598 332 - 576 7.2e-11 Leucine-rich repeat region
Pkinase PF00069 669 - 888 1.8e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 671 - 957 2.1e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016887)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28380 FvH4_6g28380
malus_domestica MD17G1226200.v1.1
prunus_persica Prupe.3G102400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0132491
rosa_laevigata RLG00000019260
rosa_multiflora Rmu_sc0005538.1_g000005
rosa_roxburghii Rroxscaffold_2G00111910
rosa_rugosa Rorug02G0304800
rosa_samantha Rh2AG357600 Rh2BG364200 Rh2CG341300 Rh2DG380900
rosa_wichuraiana Rw2G029100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2174
AarI CACCTGC 1 cut(s) 511
Acc36I ACCTGC 2 cut(s) 511, 2489
AccB7I CCANNNNNTGG 1 cut(s) 2570
AccI GTMKAC 2 cut(s) 2046, 2960
AccII CGCG 1 cut(s) 2342
AccIII TCCGGA 1 cut(s) 1540
AciI CCGC 8 cut(s) 307, 900, 1182, 1458, 1801, 1879, 1882, 2342
AclWI GGATC 8 cut(s) 146, 800, 961, 1443, 2186, 2551, 2587, 2898
AcoI YGGCCR 2 cut(s) 1117, 1197
AcsI RAATTY 9 cut(s) 157, 570, 642, 1000, 1192, 1353, 1954, 2110, 2949
AcuI CTGAAG 2 cut(s) 1962, 2918
AfaI GTAC 1 cut(s) 1280
AfiI CCNNNNNNNGG 6 cut(s) 213, 1133, 1259, 1691, 1888, 2570
AflII CTTAAG 1 cut(s) 2146
AflIII ACRYGT 3 cut(s) 1765, 2041, 2664
AgeI ACCGGT 2 cut(s) 206, 1994
AhdI GACNNNNNGTC 1 cut(s) 908
AjiI CACGTC 1 cut(s) 2042
AloI GAACNNNNNNTCC 4 cut(s) 340, 372, 1234, 1266
Alw21I GWGCWC 2 cut(s) 1407, 2736
Alw26I GTCTC 5 cut(s) 1155, 1578, 2008, 2261, 2312
AlwI GGATC 8 cut(s) 146, 800, 961, 1443, 2186, 2551, 2587, 2898
AlwNI CAGNNNCTG 1 cut(s) 2017
Ama87I CYCGRG 1 cut(s) 2630
Aor13HI TCCGGA 1 cut(s) 1540
AoxI GGCC 8 cut(s) 138, 1117, 1197, 1314, 1989, 2352, 2651, 2858
ApeKI GCWGC 8 cut(s) 116, 277, 931, 1266, 1757, 1913, 2193, 2876
ApoI RAATTY 9 cut(s) 157, 570, 642, 1000, 1192, 1353, 1954, 2110, 2949
ArsI GACNNNNNNTTYG 2 cut(s) 1771, 1803
AsiGI ACCGGT 2 cut(s) 206, 1994
Asp700I GAANNNNTTC 4 cut(s) 1030, 1422, 1947, 2953
AspS9I GGNCC 4 cut(s) 606, 994, 1314, 1389
AsuC2I CCSGG 1 cut(s) 1325
AsuHPI GGTGA 8 cut(s) 71, 276, 719, 1084, 1370, 1565, 2650, 2761
AvaI CYCGRG 1 cut(s) 2630
AvaII GGWCC 3 cut(s) 606, 994, 1389
BalI TGGCCA 2 cut(s) 1119, 1199
BanII GRGCYC 1 cut(s) 1407
BarI GAAGNNNNNNTAC 2 cut(s) 1377, 1409
Bbv12I GWGCWC 2 cut(s) 1407, 2736
BbvI GCAGC 8 cut(s) 103, 289, 918, 1253, 1744, 1900, 2205, 2863
BccI CCATC 9 cut(s) 782, 1128, 1261, 1325, 1426, 2060, 2344, 2420, 2629
BceAI ACGGC 1 cut(s) 2238
BcgI CGANNNNNNTGC 2 cut(s) 1775, 1809
BciVI GTATCC 2 cut(s) 2546, 2911
BclI TGATCA 1 cut(s) 2008
BcnI CCSGG 1 cut(s) 1325
BcoDI GTCTC 5 cut(s) 1155, 1578, 2008, 2261, 2312
BfmI CTRYAG 3 cut(s) 125, 2047, 2877
BfrI CTTAAG 1 cut(s) 2146
BfuAI ACCTGC 2 cut(s) 511, 2489
BfuI GTATCC 2 cut(s) 2546, 2911
BglII AGATCT 1 cut(s) 1978
BisI GCNGC 9 cut(s) 117, 278, 932, 1267, 1758, 1882, 1914, 2194, 2877
BlpI GCTNAGC 1 cut(s) 1406
BlsI GCNGC 9 cut(s) 118, 279, 933, 1268, 1759, 1883, 1915, 2195, 2878
Bme1390I CCNGG 1 cut(s) 1325
Bme18I GGWCC 3 cut(s) 606, 994, 1389
BmeRI GACNNNNNGTC 1 cut(s) 908
BmeT110I CYCGRG 1 cut(s) 2630
BmgBI CACGTC 1 cut(s) 2042
BmgT120I GGNCC 4 cut(s) 606, 994, 1314, 1389
BmiI GGNNCC 7 cut(s) 608, 1243, 1315, 1390, 1535, 1683, 2168
BmrFI CCNGG 1 cut(s) 1325
BmsI GCATC 3 cut(s) 238, 1704, 2863
BpmI CTGGAG 1 cut(s) 657
Bpu10I CCTNAGC 2 cut(s) 1454, 1616
Bpu1102I GCTNAGC 1 cut(s) 1406
BpuEI CTTGAG 1 cut(s) 2525
BpuMI CCSGG 1 cut(s) 1325
BsaBI GATNNNNATC 1 cut(s) 2889
BsaJI CCNNGG 3 cut(s) 1323, 1775, 2745
BsaWI WCCGGW 4 cut(s) 206, 1540, 1994, 2374
BsaXI ACNNNNNCTCC 6 cut(s) 340, 370, 1234, 1264, 2472, 2502
Bsc4I CCNNNNNNNGG 6 cut(s) 213, 1133, 1259, 1691, 1888, 2570
Bse118I RCCGGY 3 cut(s) 206, 1994, 2276
Bse1I ACTGG 3 cut(s) 640, 675, 861
Bse3DI GCAATG 1 cut(s) 2416
Bse8I GATNNNNATC 1 cut(s) 2889
BseAI TCCGGA 1 cut(s) 1540
BseDI CCNNGG 3 cut(s) 1323, 1775, 2745
BseGI GGATG 5 cut(s) 1317, 1844, 1901, 2355, 2800
BseJI GATNNNNATC 1 cut(s) 2889
BseLI CCNNNNNNNGG 6 cut(s) 213, 1133, 1259, 1691, 1888, 2570
BseMI GCAATG 1 cut(s) 2416
BseMII CTCAG 5 cut(s) 388, 1420, 2294, 2766, 2852
BseNI ACTGG 3 cut(s) 640, 675, 861
BseRI GAGGAG 3 cut(s) 860, 2496, 2499
BseXI GCAGC 8 cut(s) 103, 289, 918, 1253, 1744, 1900, 2205, 2863
BseYI CCCAGC 1 cut(s) 2694
BsgI GTGCAG 4 cut(s) 513, 1252, 2672, 2818
Bsh1236I CGCG 1 cut(s) 2342
BshFI GGCC 8 cut(s) 140, 1119, 1199, 1316, 1991, 2354, 2653, 2860
BshTI ACCGGT 2 cut(s) 206, 1994
BsiHKAI GWGCWC 2 cut(s) 1407, 2736
BsiHKCI CYCGRG 1 cut(s) 2630
BsiSI CCGG 7 cut(s) 207, 1325, 1541, 1995, 2277, 2375, 2478
BslFI GGGAC 4 cut(s) 882, 2083, 2691, 2830
BslI CCNNNNNNNGG 6 cut(s) 213, 1133, 1259, 1691, 1888, 2570
BsmAI GTCTC 5 cut(s) 1155, 1578, 2008, 2261, 2312
BsmBI CGTCTC 1 cut(s) 2261
BsmFI GGGAC 4 cut(s) 882, 2083, 2691, 2830
BsmI GAATGC 2 cut(s) 1874, 2141
BsnI GGCC 8 cut(s) 140, 1119, 1199, 1316, 1991, 2354, 2653, 2860
BsoBI CYCGRG 1 cut(s) 2630
Bsp1286I GDGCHC 2 cut(s) 1407, 2736
Bsp13I TCCGGA 1 cut(s) 1540
Bsp1407I TGTACA 1 cut(s) 1278
Bsp1720I GCTNAGC 1 cut(s) 1406
BspACI CCGC 8 cut(s) 307, 900, 1182, 1458, 1801, 1879, 1882, 2342
BspANI GGCC 8 cut(s) 140, 1119, 1199, 1316, 1991, 2354, 2653, 2860
BspCNI CTCAG 5 cut(s) 387, 1419, 2295, 2767, 2851
BspEI TCCGGA 1 cut(s) 1540
BspFNI CGCG 1 cut(s) 2342
BspLI GGNNCC 7 cut(s) 608, 1243, 1315, 1390, 1535, 1683, 2168
BspMAI CTGCAG 1 cut(s) 2881
BspMI ACCTGC 2 cut(s) 511, 2489
BspPI GGATC 8 cut(s) 146, 800, 961, 1443, 2186, 2551, 2587, 2898
BspQI GCTCTTC 2 cut(s) 126, 2299
BspTI CTTAAG 1 cut(s) 2146
BsrDI GCAATG 1 cut(s) 2416
BsrFI RCCGGY 3 cut(s) 206, 1994, 2276
BsrGI TGTACA 1 cut(s) 1278
BsrI ACTGG 3 cut(s) 640, 675, 861
BssAI RCCGGY 3 cut(s) 206, 1994, 2276
BssECI CCNNGG 3 cut(s) 1323, 1775, 2745
BssT1I CCWWGG 1 cut(s) 2745
Bst4CI ACNGT 5 cut(s) 129, 460, 1278, 1921, 2393
Bst6I CTCTTC 6 cut(s) 126, 515, 2299, 2746, 2894, 2944
BstAFI CTTAAG 1 cut(s) 2146
BstAPI GCANNNNNTGC 1 cut(s) 2933
BstAUI TGTACA 1 cut(s) 1278
BstC8I GCNNGC 3 cut(s) 1412, 2191, 2687
BstF5I GGATG 5 cut(s) 1317, 1844, 1901, 2355, 2800
BstFNI CGCG 1 cut(s) 2342
BstMAI GTCTC 5 cut(s) 1155, 1578, 2008, 2261, 2312
BstMWI GCNNNNNNNGC 6 cut(s) 1411, 2031, 2250, 2691, 2866, 2933
BstNSI RCATGY 2 cut(s) 1769, 2668
BstSCI CCNGG 1 cut(s) 1323
BstSFI CTRYAG 3 cut(s) 125, 2047, 2877
BstUI CGCG 1 cut(s) 2342
BstV1I GCAGC 8 cut(s) 103, 289, 918, 1253, 1744, 1900, 2205, 2863
BstX2I RGATCY 3 cut(s) 1435, 1978, 2543
BstXI CCANNNNNNTGG 3 cut(s) 603, 991, 2714
BstYI RGATCY 3 cut(s) 1435, 1978, 2543
BsuI GTATCC 2 cut(s) 2546, 2911
BsuRI GGCC 8 cut(s) 140, 1119, 1199, 1316, 1991, 2354, 2653, 2860
BtgZI GCGATG 2 cut(s) 2323, 2616
BtrI CACGTC 1 cut(s) 2042
BtsCI GGATG 5 cut(s) 1317, 1844, 1901, 2355, 2800
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 6 cut(s) 633, 668, 816, 1493, 1713, 2389
BveI ACCTGC 2 cut(s) 511, 2489
Cac8I GCNNGC 3 cut(s) 1412, 2191, 2687
CaiI CAGNNNCTG 1 cut(s) 2017
Cfr10I RCCGGY 3 cut(s) 206, 1994, 2276
Cfr13I GGNCC 4 cut(s) 606, 994, 1314, 1389
CseI GACGC 2 cut(s) 1272, 1507
Csp6I GTAC 1 cut(s) 1279
CspAI ACCGGT 2 cut(s) 206, 1994
CspCI CAANNNNNGTGG 2 cut(s) 397, 432
CviAII CATG 8 cut(s) 785, 1201, 1766, 1816, 2431, 2512, 2665, 2714
CviQI GTAC 1 cut(s) 1279
DriI GACNNNNNGTC 1 cut(s) 908
EaeI YGGCCR 2 cut(s) 1117, 1197
Eam1104I CTCTTC 6 cut(s) 126, 515, 2299, 2746, 2894, 2944
Eam1105I GACNNNNNGTC 1 cut(s) 908
EarI CTCTTC 6 cut(s) 126, 515, 2299, 2746, 2894, 2944
EciI GGCGGA 2 cut(s) 915, 1868
Ecl136II GAGCTC 1 cut(s) 1405
Eco130I CCWWGG 1 cut(s) 2745
Eco147I AGGCCT 1 cut(s) 2860
Eco24I GRGCYC 1 cut(s) 1407
Eco32I GATATC 3 cut(s) 1174, 1479, 2906
Eco47I GGWCC 3 cut(s) 606, 994, 1389
Eco53kI GAGCTC 1 cut(s) 1405
Eco57I CTGAAG 2 cut(s) 1962, 2918
Eco88I CYCGRG 1 cut(s) 2630
EcoICRI GAGCTC 1 cut(s) 1405
EcoRV GATATC 3 cut(s) 1174, 1479, 2906
EcoT14I CCWWGG 1 cut(s) 2745
EcoT38I GRGCYC 1 cut(s) 1407
ErhI CCWWGG 1 cut(s) 2745
Esp3I CGTCTC 1 cut(s) 2261
FaeI CATG 8 cut(s) 788, 1204, 1769, 1819, 2434, 2515, 2668, 2717
FalI AAGNNNNNCTT 4 cut(s) 1274, 1306, 1521, 1553
FaqI GGGAC 4 cut(s) 882, 2083, 2691, 2830
FatI CATG 8 cut(s) 784, 1200, 1765, 1815, 2430, 2511, 2664, 2713
FauI CCCGC 3 cut(s) 314, 1451, 1808
FauNDI CATATG 2 cut(s) 148, 1859
FbaI TGATCA 1 cut(s) 2008
FblI GTMKAC 2 cut(s) 2046, 2960
Fnu4HI GCNGC 9 cut(s) 117, 278, 932, 1267, 1758, 1882, 1914, 2194, 2877
FokI GGATG 5 cut(s) 1304, 1831, 1888, 2362, 2807
FriOI GRGCYC 1 cut(s) 1407
Fsp4HI GCNGC 9 cut(s) 117, 278, 932, 1267, 1758, 1882, 1914, 2194, 2877
GluI GCNGC 9 cut(s) 117, 278, 932, 1267, 1758, 1882, 1914, 2194, 2877
GsaI CCCAGC 1 cut(s) 2698
GsuI CTGGAG 1 cut(s) 657
HaeIII GGCC 8 cut(s) 140, 1119, 1199, 1316, 1991, 2354, 2653, 2860
HapII CCGG 7 cut(s) 207, 1325, 1541, 1995, 2277, 2375, 2478
HgaI GACGC 2 cut(s) 1272, 1507
Hin1II CATG 8 cut(s) 788, 1204, 1769, 1819, 2434, 2515, 2668, 2717
HincII GTYRAC 2 cut(s) 913, 2725
HindII GTYRAC 2 cut(s) 913, 2725
HindIII AAGCTT 2 cut(s) 986, 1283
HinfI GANTC 9 cut(s) 431, 1609, 1630, 1703, 1948, 2055, 2305, 2738, 2843
HpaII CCGG 7 cut(s) 207, 1325, 1541, 1995, 2277, 2375, 2478
HphI GGTGA 8 cut(s) 71, 276, 719, 1084, 1370, 1565, 2650, 2761
Hpy166II GTNNAC 6 cut(s) 266, 913, 2047, 2608, 2725, 2961
Hpy8I GTNNAC 6 cut(s) 266, 913, 2047, 2608, 2725, 2961
HpyCH4III ACNGT 5 cut(s) 129, 460, 1278, 1921, 2393
HpyCH4IV ACGT 1 cut(s) 2041
HpyF10VI GCNNNNNNNGC 6 cut(s) 1411, 2031, 2250, 2691, 2866, 2933
HpySE526I ACGT 1 cut(s) 2041
Hsp92II CATG 8 cut(s) 788, 1204, 1769, 1819, 2434, 2515, 2668, 2717
Kpn2I TCCGGA 1 cut(s) 1540
Ksp22I TGATCA 1 cut(s) 2008
LguI GCTCTTC 2 cut(s) 126, 2299
LmnI GCTCC 5 cut(s) 322, 847, 1533, 1802, 2031
Lsp1109I GCAGC 8 cut(s) 103, 289, 918, 1253, 1744, 1900, 2205, 2863
LweI GCATC 3 cut(s) 238, 1704, 2863
MaeII ACGT 1 cut(s) 2041
MaeIII GTNAC 9 cut(s) 49, 238, 314, 463, 1053, 1966, 2091, 2393, 2638
MflI RGATCY 3 cut(s) 1435, 1978, 2543
MhlI GDGCHC 2 cut(s) 1407, 2736
MlsI TGGCCA 2 cut(s) 1119, 1199
MluNI TGGCCA 2 cut(s) 1119, 1199
MlyI GAGTC 5 cut(s) 1639, 2064, 2314, 2732, 2852
MmeI TCCRAC 2 cut(s) 540, 573
Mox20I TGGCCA 2 cut(s) 1119, 1199
MroI TCCGGA 1 cut(s) 1540
MroXI GAANNNNTTC 4 cut(s) 1030, 1422, 1947, 2953
MscI TGGCCA 2 cut(s) 1119, 1199
MseI TTAA 5 cut(s) 530, 1662, 1887, 2127, 2147
MslI CAYNNNNRTG 6 cut(s) 234, 342, 1743, 1814, 1889, 2712
Msp20I TGGCCA 2 cut(s) 1119, 1199
MspA1I CMGCKG 1 cut(s) 2694
MspCI CTTAAG 1 cut(s) 2146
MspI CCGG 7 cut(s) 207, 1325, 1541, 1995, 2277, 2375, 2478
MspR9I CCNGG 1 cut(s) 1325
Mva1269I GAATGC 2 cut(s) 1874, 2141
MvnI CGCG 1 cut(s) 2342
MwoI GCNNNNNNNGC 6 cut(s) 1411, 2031, 2250, 2691, 2866, 2933
NciI CCSGG 1 cut(s) 1325
NdeI CATATG 2 cut(s) 148, 1859
NlaIII CATG 8 cut(s) 788, 1204, 1769, 1819, 2434, 2515, 2668, 2717
NlaIV GGNNCC 7 cut(s) 608, 1243, 1315, 1390, 1535, 1683, 2168
NmuCI GTSAC 3 cut(s) 1053, 2393, 2638
NspI RCATGY 2 cut(s) 1769, 2668
PaeR7I CTCGAG 1 cut(s) 2630
PaqCI CACCTGC 1 cut(s) 511
PceI AGGCCT 1 cut(s) 2860
PciI ACATGT 2 cut(s) 1765, 2664
PciSI GCTCTTC 2 cut(s) 126, 2299
PctI GAATGC 2 cut(s) 1874, 2141
PdmI GAANNNNTTC 4 cut(s) 1030, 1422, 1947, 2953
PfeI GAWTC 4 cut(s) 431, 1609, 1703, 1948
PflMI CCANNNNNTGG 1 cut(s) 2570
PinAI ACCGGT 2 cut(s) 206, 1994
PkrI GCNGC 9 cut(s) 118, 279, 933, 1268, 1759, 1883, 1915, 2195, 2878
PleI GAGTC 5 cut(s) 1638, 2063, 2313, 2732, 2851
PpsI GAGTC 5 cut(s) 1638, 2063, 2313, 2732, 2851
PscI ACATGT 2 cut(s) 1765, 2664
PsiI TTATAA 1 cut(s) 2174
Psp124BI GAGCTC 1 cut(s) 1407
PspFI CCCAGC 1 cut(s) 2694
PspN4I GGNNCC 7 cut(s) 608, 1243, 1315, 1390, 1535, 1683, 2168
PspPI GGNCC 4 cut(s) 606, 994, 1314, 1389
PsrI GAACNNNNNNTAC 2 cut(s) 354, 386
PstI CTGCAG 1 cut(s) 2881
PstNI CAGNNNCTG 1 cut(s) 2017
PsuI RGATCY 3 cut(s) 1435, 1978, 2543
PvuII CAGCTG 1 cut(s) 2694
RsaI GTAC 1 cut(s) 1280
RsaNI GTAC 1 cut(s) 1279
RseI CAYNNNNRTG 6 cut(s) 234, 342, 1743, 1814, 1889, 2712
SacI GAGCTC 1 cut(s) 1407
SapI GCTCTTC 2 cut(s) 126, 2299
SaqAI TTAA 5 cut(s) 530, 1662, 1887, 2127, 2147
SatI GCNGC 9 cut(s) 117, 278, 932, 1267, 1758, 1882, 1914, 2194, 2877
Sau96I GGNCC 4 cut(s) 606, 994, 1314, 1389
SchI GAGTC 5 cut(s) 1639, 2064, 2314, 2732, 2852
ScrFI CCNGG 1 cut(s) 1325
SduI GDGCHC 2 cut(s) 1407, 2736
SfaNI GCATC 3 cut(s) 238, 1704, 2863
SfcI CTRYAG 3 cut(s) 125, 2047, 2877
Sfr274I CTCGAG 1 cut(s) 2630
SgrAI CRCCGGYG 2 cut(s) 206, 1994
SinI GGWCC 3 cut(s) 606, 994, 1389
SlaI CTCGAG 1 cut(s) 2630
SmiMI CAYNNNNRTG 6 cut(s) 234, 342, 1743, 1814, 1889, 2712
SmlI CTYRAG 3 cut(s) 2146, 2504, 2630
SmoI CTYRAG 3 cut(s) 2146, 2504, 2630
SseBI AGGCCT 1 cut(s) 2860
SsiI CCGC 8 cut(s) 307, 900, 1182, 1458, 1801, 1879, 1882, 2342
SspI AATATT 2 cut(s) 882, 2326
SstI GAGCTC 1 cut(s) 1407
StuI AGGCCT 1 cut(s) 2860
StyD4I CCNGG 1 cut(s) 1323
StyI CCWWGG 1 cut(s) 2745
TaaI ACNGT 5 cut(s) 129, 460, 1278, 1921, 2393
TaiI ACGT 1 cut(s) 2044
TaqI TCGA 6 cut(s) 540, 804, 2582, 2631, 2736, 2871
TatI WGTACW 1 cut(s) 1278
TauI GCSGC 1 cut(s) 1884
TfiI GAWTC 4 cut(s) 431, 1609, 1703, 1948
Tru1I TTAA 5 cut(s) 530, 1662, 1887, 2127, 2147
Tru9I TTAA 5 cut(s) 530, 1662, 1887, 2127, 2147
TscAI CASTG 6 cut(s) 640, 675, 823, 1500, 1713, 2396
TseFI GTSAC 3 cut(s) 1053, 2393, 2638
TseI GCWGC 8 cut(s) 116, 277, 931, 1266, 1757, 1913, 2193, 2876
Tsp45I GTSAC 3 cut(s) 1053, 2393, 2638
TspGWI ACGGA 1 cut(s) 409
TspRI CASTG 6 cut(s) 640, 675, 823, 1500, 1713, 2396
Van91I CCANNNNNTGG 1 cut(s) 2570
Vha464I CTTAAG 1 cut(s) 2146
VpaK11BI GGWCC 3 cut(s) 606, 994, 1389
XapI RAATTY 9 cut(s) 157, 570, 642, 1000, 1192, 1353, 1954, 2110, 2949
XbaI TCTAGA 2 cut(s) 841, 2759
XceI RCATGY 2 cut(s) 1769, 2668
XhoI CTCGAG 1 cut(s) 2630
XmiI GTMKAC 2 cut(s) 2046, 2960
XmnI GAANNNNTTC 4 cut(s) 1030, 1422, 1947, 2953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.