Rh2DG380900
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0016887
Physical Location & Seq
Forward (+)
54855830 .. 54859026
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG380900.1

Sequence Viewer

Length: 3030 bp
ATGAGAGTAGAAGAGAAGAAAATTATTGTCAGGCTCTTTCAGTTTACCAGTATGGTCTTCATCAAAACCATTTTCTTTCTTCTATTCCAGCATTTTGTTGTTACATCTTGTAATGCAGTTATAGGTCATCACCACAATCCTCATCACTCTTTGCTTAGAGATAAGGCTGCTCTTCTACAGTTCAAGAAGGCCATTACATATGATCCAAATTCCACTCTAGCTAATTGGAATGAAGCTACTGATGTTTGCAACTACACCGGTGTTGGGTGCAACAAAAAGCATCATCGTGTAACTATACTCCTTCTGCATAATCGTAAACTTGTAGGGCAGCTTTCACCTATCATTTCAAATCTAACCCGCCTTCGTTACTTGGAGCTTGTAGGCAATCACTTTTATGGAACTATCCCTCCTGAACTTTCCTTACTCAGACACCTACATCATCTACGGATTGAGGGGAACAACTTTCGTGGTTCTATACCAGATTCCTTAGTCCTTCCTTCACAACTCACAGTTGTTACTCTTTTGCAAAACAATTTCTCTGGTGCAGTTCCACCTGCTCTCTTCTCCAACTGCTCTCTGTTAAGGGTGTTCGACCTTTCCAACAACTTTCTTTCAGGGAAAATTCCAAGAGAAATTGGAAATTGTCCAAACCTATGGACCCTCAATTTATACAACAATCAATTCACTGGAGAAATTCCTCTTTCTTTGACTAATACCTCACTGGTCAATTTAGATGTTGAGTTCAATCATCTTTCAGGTGAATTGCCCGTCAAACTTGTGGAAAACTTGCCTGATATTCTCTATCTTCACTTATCAAATAATAATATGGTAAGCCATGATGGAAACACCAATCTCGATCCATTCTTCACTGCCCTTGCAAATTGCATTAGTCTAGAGGAGCTTGAACTGGCGAGTATGGGACTTGGAGGAATATTACCTAGTTCCATTGGCGGACTTGGTGTCAACTTTACATACCTGTTGCTGCAAGAAAACAAGATCATTGGATCAGTTCCTTCAAATATAGGTAATTTATCCAAGCTTGTGGTCCTGAATTTGACCTCCAATCTTATGAATGGAACAATTTCAGCAAACATAAGTCAGTTGTCACACTTGGAACAGCTTTTCTTATCTCACAACTTTTTCACCAGCACGATACCAGAAGCACTTGGCCATCTTCCTCATCTTGGTCTGCTTGATCTGTCTCATAATTCGTTCTTCGGGGATATCCCAAGCGGTCTAGGAAATTTGGCCATGCTGAATTATCTGTTCCTCAACAACAACCTCCTATCTGGAACCATTCCTCCATCATTAGGACGCTGCACAGAACTGTACAAGCTTGACTTATCATTCAACAGATTGGCAGGGGCCATCCCCCCGGAATTATCAGGTTTGAATGAGATCAGAATTTTCATAAACCTCTCACACAATCACCTTGAAGGGTCTATACCGATTGAGCTCAGCAAGCTAGAAGATGTTCAAGAGATGGATCTCTCATCAAATAACCTAAGCGGGAGTATCTTTCCACAGATATCAAGCTGTATCGCACTGACGCTCATAAACTTCTCACACAATGCTTTGGAAGGGAGCCTTCCGGATTTCATAGGTGAGCTAAAGAACCTTGTGTCTCTTGATGTTTCAGATAACCACTTGTCAGGAAGGATTCCCCTAAGCCTCAACAAGAGTCTAACACTCACATATCTAAATCTTTCATTTAATGACTTCAAAGGAATGGTTCCTTCTGGTGGCATCTTTGAATCAGTGTCATATACCTCTTTCTTAGGCAATAAACATCTTTGTGGTAAAGTTGCTGCGAAACATGTTTGCCCTCGGACAAAGCATTTGTTTCGCTCCCGCATATTCATAATCATGTTTGTCATAGTCATATTCATTTCAACATCCTTATCAATCATATGCTGTGTGATTGCATTCCGCCGCATTAAGGTGCTGATTTCATCCCAGAGGGCTGCAACCGTAAAGAAACCAACACAACCTGAAGTGATTCAGAATTTCCCAAGAGTTACATACCGAGATCTGTCAGAGGCCACCGGTGGATTTGATGATCAGAGACTGATTGGCACGGGGAGCTATGGACGTGTCTACAGGGGAGTCCTTCCAGATGGGACAACCATAGCAGTCAAGGTGTTACATTTACAATCTGGAAATTCAACAAAAAGTTTTAACAGAGAATGCCAAGTCTTAAGGAGAATTAGGCACAGGAACCTTATAAGGATCATAACAGCTTGCAGCCTACCTGATTTCAAGGCTATTGTTCTTCCTTATATGGCAAATGGTAGCTTGGATAGCCGTCTCTATCCACATTCACAAGCCGGTCTAGGTTCAGGCTCTTCTGATCTGAGTCTCATTCAGAGGGTCAATATTTGTAGCGACATCGCGGAAGGGATGGCCTATCTTCATCATTACTCTCCGGTCAGAGTTATACACTGTGACCTGAAACCAAGCAATGTTCTTCTCAACGATGGCATGACTGCTTTAGTTTCTGATTTTGGGATTGCAAGATTAGTGATAGCCGGAGGAGGAGTGGGAAATGCAGGTCTTGAGAACATGGGAAACTCTACTGCAAGTTTATTATGTGGATCTATTGGATACATAGCACCAGAGTATGGGTTTGGATCGAACACATCTACAAAAGGCGATGTTTACAGCTTCGGTGTTCTTGTTCTCGAGATGGTGACAAGAAAAAGGCCGACAGATGACATGTTTGTTGGGGGACTAAGCCTGCACAGCTGGGTAAAGAACCATTATCATGGAAGGGTTGACAAAGTGCTCGACTCTTCCTTGGTGAGAGCTTCTAGAGATCAGCCACCTGAGATGAAAAAAATGTGGGATGTTGCCATAGGAGAAATGATAGAGTTGGGTATTCTCTGCACTCAGGAGTCCCCTTCCACTAGGCCTACAATGCTCGATGCTGCAGATGATTTGGATCGGCTGAAGAGATATCTTGGTGGGGATACTACTGCAACATTTGCCTCTTCATTAGGAATTTCTTCGTCTACCCTAGATGACGACTAG

Protein Analysis

1009

Amino Acids

110.87

Weight (kDa)

7.24

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 53 - 91 9.8e-12 Leucine rich repeat N-terminal domain
LRR_8 PF13855 169 - 228 6.7e-08 Leucine rich repeat
LRR_8 PF13855 346 - 404 7.7e-07 Leucine rich repeat
LRR_14 PF23598 349 - 593 7.3e-11 Leucine-rich repeat region
Pkinase PF00069 686 - 905 1.9e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 688 - 974 2.2e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016887)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28380 FvH4_6g28380
malus_domestica MD17G1226200.v1.1
prunus_persica Prupe.3G102400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0132491
rosa_laevigata RLG00000019260
rosa_multiflora Rmu_sc0005538.1_g000005
rosa_roxburghii Rroxscaffold_2G00111910
rosa_rugosa Rorug02G0304800
rosa_samantha Rh2AG357600 Rh2BG364200 Rh2CG341300 Rh2DG380900
rosa_wichuraiana Rw2G029100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2225
AarI CACCTGC 1 cut(s) 562
Acc36I ACCTGC 2 cut(s) 562, 2540
AccB7I CCANNNNNTGG 1 cut(s) 2621
AccI GTMKAC 2 cut(s) 2097, 3011
AccII CGCG 1 cut(s) 2393
AccIII TCCGGA 1 cut(s) 1591
AciI CCGC 8 cut(s) 358, 951, 1233, 1509, 1852, 1930, 1933, 2393
AclWI GGATC 8 cut(s) 197, 851, 1012, 1494, 2237, 2602, 2638, 2949
AcoI YGGCCR 2 cut(s) 1168, 1248
AcsI RAATTY 9 cut(s) 208, 621, 693, 1051, 1243, 1404, 2005, 2161, 3000
AcuI CTGAAG 2 cut(s) 2013, 2969
AfaI GTAC 1 cut(s) 1331
AfiI CCNNNNNNNGG 6 cut(s) 264, 1184, 1310, 1742, 1939, 2621
AflII CTTAAG 1 cut(s) 2197
AflIII ACRYGT 3 cut(s) 1816, 2092, 2715
AgeI ACCGGT 2 cut(s) 257, 2045
AhdI GACNNNNNGTC 1 cut(s) 959
AjiI CACGTC 1 cut(s) 2093
AloI GAACNNNNNNTCC 4 cut(s) 391, 423, 1285, 1317
Alw21I GWGCWC 2 cut(s) 1458, 2787
Alw26I GTCTC 5 cut(s) 1206, 1629, 2059, 2312, 2363
AlwI GGATC 8 cut(s) 197, 851, 1012, 1494, 2237, 2602, 2638, 2949
AlwNI CAGNNNCTG 1 cut(s) 2068
Ama87I CYCGRG 1 cut(s) 2681
Aor13HI TCCGGA 1 cut(s) 1591
AoxI GGCC 8 cut(s) 189, 1168, 1248, 1365, 2040, 2403, 2702, 2909
ApeKI GCWGC 8 cut(s) 167, 328, 982, 1317, 1808, 1964, 2244, 2927
ApoI RAATTY 9 cut(s) 208, 621, 693, 1051, 1243, 1404, 2005, 2161, 3000
ArsI GACNNNNNNTTYG 2 cut(s) 1822, 1854
AsiGI ACCGGT 2 cut(s) 257, 2045
Asp700I GAANNNNTTC 4 cut(s) 1081, 1473, 1998, 3004
AspS9I GGNCC 3 cut(s) 657, 1045, 1365
AsuC2I CCSGG 1 cut(s) 1376
AsuHPI GGTGA 8 cut(s) 122, 327, 770, 1135, 1421, 1616, 2701, 2812
AvaI CYCGRG 1 cut(s) 2681
AvaII GGWCC 2 cut(s) 657, 1045
BalI TGGCCA 2 cut(s) 1170, 1250
BanII GRGCYC 1 cut(s) 1458
BarI GAAGNNNNNNTAC 2 cut(s) 1428, 1460
BbsI GAAGAC 1 cut(s) 49
Bbv12I GWGCWC 2 cut(s) 1458, 2787
BbvI GCAGC 8 cut(s) 154, 340, 969, 1304, 1795, 1951, 2256, 2914
BccI CCATC 9 cut(s) 833, 1179, 1312, 1376, 1477, 2111, 2395, 2471, 2680
BceAI ACGGC 1 cut(s) 2289
BcgI CGANNNNNNTGC 2 cut(s) 1826, 1860
BciVI GTATCC 2 cut(s) 2597, 2962
BclI TGATCA 1 cut(s) 2059
BcnI CCSGG 1 cut(s) 1376
BcoDI GTCTC 5 cut(s) 1206, 1629, 2059, 2312, 2363
BfmI CTRYAG 3 cut(s) 176, 2098, 2928
BfrI CTTAAG 1 cut(s) 2197
BfuAI ACCTGC 2 cut(s) 562, 2540
BfuI GTATCC 2 cut(s) 2597, 2962
BglII AGATCT 1 cut(s) 2029
BisI GCNGC 9 cut(s) 168, 329, 983, 1318, 1809, 1933, 1965, 2245, 2928
BlpI GCTNAGC 1 cut(s) 1457
BlsI GCNGC 9 cut(s) 169, 330, 984, 1319, 1810, 1934, 1966, 2246, 2929
Bme1390I CCNGG 1 cut(s) 1376
Bme18I GGWCC 2 cut(s) 657, 1045
BmeRI GACNNNNNGTC 1 cut(s) 959
BmeT110I CYCGRG 1 cut(s) 2681
BmgBI CACGTC 1 cut(s) 2093
BmgT120I GGNCC 3 cut(s) 657, 1045, 1365
BmiI GGNNCC 6 cut(s) 659, 1294, 1366, 1586, 1734, 2219
BmrFI CCNGG 1 cut(s) 1376
BmsI GCATC 3 cut(s) 289, 1755, 2914
BpiI GAAGAC 1 cut(s) 49
BpmI CTGGAG 1 cut(s) 708
Bpu10I CCTNAGC 2 cut(s) 1505, 1667
Bpu1102I GCTNAGC 1 cut(s) 1457
BpuEI CTTGAG 1 cut(s) 2576
BpuMI CCSGG 1 cut(s) 1376
BsaBI GATNNNNATC 1 cut(s) 2940
BsaJI CCNNGG 3 cut(s) 1374, 1826, 2796
BsaWI WCCGGW 4 cut(s) 257, 1591, 2045, 2425
BsaXI ACNNNNNCTCC 6 cut(s) 391, 421, 1285, 1315, 2523, 2553
Bsc4I CCNNNNNNNGG 6 cut(s) 264, 1184, 1310, 1742, 1939, 2621
Bse118I RCCGGY 3 cut(s) 257, 2045, 2327
Bse1I ACTGG 4 cut(s) 48, 691, 726, 912
Bse3DI GCAATG 1 cut(s) 2467
Bse8I GATNNNNATC 1 cut(s) 2940
BseAI TCCGGA 1 cut(s) 1591
BseDI CCNNGG 3 cut(s) 1374, 1826, 2796
BseGI GGATG 5 cut(s) 1368, 1895, 1952, 2406, 2851
BseJI GATNNNNATC 1 cut(s) 2940
BseLI CCNNNNNNNGG 6 cut(s) 264, 1184, 1310, 1742, 1939, 2621
BseMI GCAATG 1 cut(s) 2467
BseMII CTCAG 5 cut(s) 439, 1471, 2345, 2817, 2903
BseNI ACTGG 4 cut(s) 48, 691, 726, 912
BseRI GAGGAG 3 cut(s) 911, 2547, 2550
BseXI GCAGC 8 cut(s) 154, 340, 969, 1304, 1795, 1951, 2256, 2914
BseYI CCCAGC 1 cut(s) 2745
BsgI GTGCAG 4 cut(s) 564, 1303, 2723, 2869
Bsh1236I CGCG 1 cut(s) 2393
BshFI GGCC 8 cut(s) 191, 1170, 1250, 1367, 2042, 2405, 2704, 2911
BshTI ACCGGT 2 cut(s) 257, 2045
BsiHKAI GWGCWC 2 cut(s) 1458, 2787
BsiHKCI CYCGRG 1 cut(s) 2681
BsiSI CCGG 7 cut(s) 258, 1376, 1592, 2046, 2328, 2426, 2529
BslFI GGGAC 4 cut(s) 933, 2134, 2742, 2881
BslI CCNNNNNNNGG 6 cut(s) 264, 1184, 1310, 1742, 1939, 2621
BsmAI GTCTC 5 cut(s) 1206, 1629, 2059, 2312, 2363
BsmBI CGTCTC 1 cut(s) 2312
BsmFI GGGAC 4 cut(s) 933, 2134, 2742, 2881
BsmI GAATGC 2 cut(s) 1925, 2192
BsnI GGCC 8 cut(s) 191, 1170, 1250, 1367, 2042, 2405, 2704, 2911
BsoBI CYCGRG 1 cut(s) 2681
Bsp1286I GDGCHC 2 cut(s) 1458, 2787
Bsp13I TCCGGA 1 cut(s) 1591
Bsp1407I TGTACA 1 cut(s) 1329
Bsp1720I GCTNAGC 1 cut(s) 1457
BspACI CCGC 8 cut(s) 358, 951, 1233, 1509, 1852, 1930, 1933, 2393
BspANI GGCC 8 cut(s) 191, 1170, 1250, 1367, 2042, 2405, 2704, 2911
BspCNI CTCAG 5 cut(s) 438, 1470, 2346, 2818, 2902
BspEI TCCGGA 1 cut(s) 1591
BspFNI CGCG 1 cut(s) 2393
BspLI GGNNCC 6 cut(s) 659, 1294, 1366, 1586, 1734, 2219
BspMAI CTGCAG 1 cut(s) 2932
BspMI ACCTGC 2 cut(s) 562, 2540
BspPI GGATC 8 cut(s) 197, 851, 1012, 1494, 2237, 2602, 2638, 2949
BspQI GCTCTTC 2 cut(s) 177, 2350
BspTI CTTAAG 1 cut(s) 2197
BsrDI GCAATG 1 cut(s) 2467
BsrFI RCCGGY 3 cut(s) 257, 2045, 2327
BsrGI TGTACA 1 cut(s) 1329
BsrI ACTGG 4 cut(s) 48, 691, 726, 912
BssAI RCCGGY 3 cut(s) 257, 2045, 2327
BssECI CCNNGG 3 cut(s) 1374, 1826, 2796
BssT1I CCWWGG 1 cut(s) 2796
Bst4CI ACNGT 5 cut(s) 180, 511, 1329, 1972, 2444
Bst6I CTCTTC 7 cut(s) 6, 177, 566, 2350, 2797, 2945, 2995
BstAFI CTTAAG 1 cut(s) 2197
BstAPI GCANNNNNTGC 1 cut(s) 2984
BstAUI TGTACA 1 cut(s) 1329
BstC8I GCNNGC 3 cut(s) 1463, 2242, 2738
BstF5I GGATG 5 cut(s) 1368, 1895, 1952, 2406, 2851
BstFNI CGCG 1 cut(s) 2393
BstMAI GTCTC 5 cut(s) 1206, 1629, 2059, 2312, 2363
BstMWI GCNNNNNNNGC 6 cut(s) 1462, 2082, 2301, 2742, 2917, 2984
BstNSI RCATGY 2 cut(s) 1820, 2719
BstSCI CCNGG 1 cut(s) 1374
BstSFI CTRYAG 3 cut(s) 176, 2098, 2928
BstUI CGCG 1 cut(s) 2393
BstV1I GCAGC 8 cut(s) 154, 340, 969, 1304, 1795, 1951, 2256, 2914
BstV2I GAAGAC 1 cut(s) 49
BstX2I RGATCY 3 cut(s) 1486, 2029, 2594
BstXI CCANNNNNNTGG 3 cut(s) 654, 1042, 2765
BstYI RGATCY 3 cut(s) 1486, 2029, 2594
BsuI GTATCC 2 cut(s) 2597, 2962
BsuRI GGCC 8 cut(s) 191, 1170, 1250, 1367, 2042, 2405, 2704, 2911
BtgZI GCGATG 2 cut(s) 2374, 2667
BtrI CACGTC 1 cut(s) 2093
BtsCI GGATG 5 cut(s) 1368, 1895, 1952, 2406, 2851
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 6 cut(s) 684, 719, 867, 1544, 1764, 2440
BveI ACCTGC 2 cut(s) 562, 2540
Cac8I GCNNGC 3 cut(s) 1463, 2242, 2738
CaiI CAGNNNCTG 1 cut(s) 2068
Cfr10I RCCGGY 3 cut(s) 257, 2045, 2327
Cfr13I GGNCC 3 cut(s) 657, 1045, 1365
CseI GACGC 2 cut(s) 1323, 1558
Csp6I GTAC 1 cut(s) 1330
CspAI ACCGGT 2 cut(s) 257, 2045
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviAII CATG 8 cut(s) 836, 1252, 1817, 1867, 2482, 2563, 2716, 2765
CviQI GTAC 1 cut(s) 1330
DriI GACNNNNNGTC 1 cut(s) 959
EaeI YGGCCR 2 cut(s) 1168, 1248
Eam1104I CTCTTC 7 cut(s) 6, 177, 566, 2350, 2797, 2945, 2995
Eam1105I GACNNNNNGTC 1 cut(s) 959
EarI CTCTTC 7 cut(s) 6, 177, 566, 2350, 2797, 2945, 2995
EciI GGCGGA 2 cut(s) 966, 1919
Ecl136II GAGCTC 1 cut(s) 1456
Eco130I CCWWGG 1 cut(s) 2796
Eco147I AGGCCT 1 cut(s) 2911
Eco24I GRGCYC 1 cut(s) 1458
Eco32I GATATC 3 cut(s) 1225, 1530, 2957
Eco47I GGWCC 2 cut(s) 657, 1045
Eco53kI GAGCTC 1 cut(s) 1456
Eco57I CTGAAG 2 cut(s) 2013, 2969
Eco88I CYCGRG 1 cut(s) 2681
EcoICRI GAGCTC 1 cut(s) 1456
EcoRV GATATC 3 cut(s) 1225, 1530, 2957
EcoT14I CCWWGG 1 cut(s) 2796
EcoT38I GRGCYC 1 cut(s) 1458
ErhI CCWWGG 1 cut(s) 2796
Esp3I CGTCTC 1 cut(s) 2312
FaeI CATG 8 cut(s) 839, 1255, 1820, 1870, 2485, 2566, 2719, 2768
FalI AAGNNNNNCTT 4 cut(s) 1325, 1357, 1572, 1604
FaqI GGGAC 4 cut(s) 933, 2134, 2742, 2881
FatI CATG 8 cut(s) 835, 1251, 1816, 1866, 2481, 2562, 2715, 2764
FauI CCCGC 3 cut(s) 365, 1502, 1859
FauNDI CATATG 2 cut(s) 199, 1910
FbaI TGATCA 1 cut(s) 2059
FblI GTMKAC 2 cut(s) 2097, 3011
Fnu4HI GCNGC 9 cut(s) 168, 329, 983, 1318, 1809, 1933, 1965, 2245, 2928
FokI GGATG 5 cut(s) 1355, 1882, 1939, 2413, 2858
FriOI GRGCYC 1 cut(s) 1458
Fsp4HI GCNGC 9 cut(s) 168, 329, 983, 1318, 1809, 1933, 1965, 2245, 2928
GluI GCNGC 9 cut(s) 168, 329, 983, 1318, 1809, 1933, 1965, 2245, 2928
GsaI CCCAGC 1 cut(s) 2749
GsuI CTGGAG 1 cut(s) 708
HaeIII GGCC 8 cut(s) 191, 1170, 1250, 1367, 2042, 2405, 2704, 2911
HapII CCGG 7 cut(s) 258, 1376, 1592, 2046, 2328, 2426, 2529
HgaI GACGC 2 cut(s) 1323, 1558
Hin1II CATG 8 cut(s) 839, 1255, 1820, 1870, 2485, 2566, 2719, 2768
HincII GTYRAC 2 cut(s) 964, 2776
HindII GTYRAC 2 cut(s) 964, 2776
HindIII AAGCTT 2 cut(s) 1037, 1334
HinfI GANTC 9 cut(s) 482, 1660, 1681, 1754, 1999, 2106, 2356, 2789, 2894
HpaII CCGG 7 cut(s) 258, 1376, 1592, 2046, 2328, 2426, 2529
HphI GGTGA 8 cut(s) 122, 327, 770, 1135, 1421, 1616, 2701, 2812
Hpy166II GTNNAC 7 cut(s) 45, 317, 964, 2098, 2659, 2776, 3012
Hpy8I GTNNAC 7 cut(s) 45, 317, 964, 2098, 2659, 2776, 3012
HpyCH4III ACNGT 5 cut(s) 180, 511, 1329, 1972, 2444
HpyCH4IV ACGT 1 cut(s) 2092
HpyF10VI GCNNNNNNNGC 6 cut(s) 1462, 2082, 2301, 2742, 2917, 2984
HpySE526I ACGT 1 cut(s) 2092
Hsp92II CATG 8 cut(s) 839, 1255, 1820, 1870, 2485, 2566, 2719, 2768
Kpn2I TCCGGA 1 cut(s) 1591
Ksp22I TGATCA 1 cut(s) 2059
LguI GCTCTTC 2 cut(s) 177, 2350
LmnI GCTCC 5 cut(s) 373, 898, 1584, 1853, 2082
Lsp1109I GCAGC 8 cut(s) 154, 340, 969, 1304, 1795, 1951, 2256, 2914
LweI GCATC 3 cut(s) 289, 1755, 2914
MaeII ACGT 1 cut(s) 2092
MaeIII GTNAC 9 cut(s) 100, 289, 365, 514, 1104, 2017, 2142, 2444, 2689
MflI RGATCY 3 cut(s) 1486, 2029, 2594
MhlI GDGCHC 2 cut(s) 1458, 2787
MlsI TGGCCA 2 cut(s) 1170, 1250
MluNI TGGCCA 2 cut(s) 1170, 1250
MlyI GAGTC 5 cut(s) 1690, 2115, 2365, 2783, 2903
MmeI TCCRAC 2 cut(s) 591, 624
Mox20I TGGCCA 2 cut(s) 1170, 1250
MroI TCCGGA 1 cut(s) 1591
MroXI GAANNNNTTC 4 cut(s) 1081, 1473, 1998, 3004
MscI TGGCCA 2 cut(s) 1170, 1250
MseI TTAA 5 cut(s) 581, 1713, 1938, 2178, 2198
MslI CAYNNNNRTG 6 cut(s) 285, 393, 1794, 1865, 1940, 2763
Msp20I TGGCCA 2 cut(s) 1170, 1250
MspA1I CMGCKG 1 cut(s) 2745
MspCI CTTAAG 1 cut(s) 2197
MspI CCGG 7 cut(s) 258, 1376, 1592, 2046, 2328, 2426, 2529
MspR9I CCNGG 1 cut(s) 1376
Mva1269I GAATGC 2 cut(s) 1925, 2192
MvnI CGCG 1 cut(s) 2393
MwoI GCNNNNNNNGC 6 cut(s) 1462, 2082, 2301, 2742, 2917, 2984
NciI CCSGG 1 cut(s) 1376
NdeI CATATG 2 cut(s) 199, 1910
NlaIII CATG 8 cut(s) 839, 1255, 1820, 1870, 2485, 2566, 2719, 2768
NlaIV GGNNCC 6 cut(s) 659, 1294, 1366, 1586, 1734, 2219
NmuCI GTSAC 3 cut(s) 1104, 2444, 2689
NspI RCATGY 2 cut(s) 1820, 2719
PaeR7I CTCGAG 1 cut(s) 2681
PaqCI CACCTGC 1 cut(s) 562
PceI AGGCCT 1 cut(s) 2911
PciI ACATGT 2 cut(s) 1816, 2715
PciSI GCTCTTC 2 cut(s) 177, 2350
PctI GAATGC 2 cut(s) 1925, 2192
PdmI GAANNNNTTC 4 cut(s) 1081, 1473, 1998, 3004
PfeI GAWTC 4 cut(s) 482, 1660, 1754, 1999
PflMI CCANNNNNTGG 1 cut(s) 2621
PinAI ACCGGT 2 cut(s) 257, 2045
PkrI GCNGC 9 cut(s) 169, 330, 984, 1319, 1810, 1934, 1966, 2246, 2929
PleI GAGTC 5 cut(s) 1689, 2114, 2364, 2783, 2902
PpsI GAGTC 5 cut(s) 1689, 2114, 2364, 2783, 2902
PscI ACATGT 2 cut(s) 1816, 2715
PsiI TTATAA 1 cut(s) 2225
Psp124BI GAGCTC 1 cut(s) 1458
PspFI CCCAGC 1 cut(s) 2745
PspN4I GGNNCC 6 cut(s) 659, 1294, 1366, 1586, 1734, 2219
PspPI GGNCC 3 cut(s) 657, 1045, 1365
PsrI GAACNNNNNNTAC 2 cut(s) 405, 437
PstI CTGCAG 1 cut(s) 2932
PstNI CAGNNNCTG 1 cut(s) 2068
PsuI RGATCY 3 cut(s) 1486, 2029, 2594
PvuII CAGCTG 1 cut(s) 2745
RsaI GTAC 1 cut(s) 1331
RsaNI GTAC 1 cut(s) 1330
RseI CAYNNNNRTG 6 cut(s) 285, 393, 1794, 1865, 1940, 2763
SacI GAGCTC 1 cut(s) 1458
SapI GCTCTTC 2 cut(s) 177, 2350
SaqAI TTAA 5 cut(s) 581, 1713, 1938, 2178, 2198
SatI GCNGC 9 cut(s) 168, 329, 983, 1318, 1809, 1933, 1965, 2245, 2928
Sau96I GGNCC 3 cut(s) 657, 1045, 1365
SchI GAGTC 5 cut(s) 1690, 2115, 2365, 2783, 2903
ScrFI CCNGG 1 cut(s) 1376
SduI GDGCHC 2 cut(s) 1458, 2787
SfaNI GCATC 3 cut(s) 289, 1755, 2914
SfcI CTRYAG 3 cut(s) 176, 2098, 2928
Sfr274I CTCGAG 1 cut(s) 2681
SgrAI CRCCGGYG 2 cut(s) 257, 2045
SinI GGWCC 2 cut(s) 657, 1045
SlaI CTCGAG 1 cut(s) 2681
SmiMI CAYNNNNRTG 6 cut(s) 285, 393, 1794, 1865, 1940, 2763
SmlI CTYRAG 3 cut(s) 2197, 2555, 2681
SmoI CTYRAG 3 cut(s) 2197, 2555, 2681
SseBI AGGCCT 1 cut(s) 2911
SsiI CCGC 8 cut(s) 358, 951, 1233, 1509, 1852, 1930, 1933, 2393
SspI AATATT 2 cut(s) 933, 2377
SstI GAGCTC 1 cut(s) 1458
StuI AGGCCT 1 cut(s) 2911
StyD4I CCNGG 1 cut(s) 1374
StyI CCWWGG 1 cut(s) 2796
TaaI ACNGT 5 cut(s) 180, 511, 1329, 1972, 2444
TaiI ACGT 1 cut(s) 2095
TaqI TCGA 6 cut(s) 591, 855, 2633, 2682, 2787, 2922
TatI WGTACW 1 cut(s) 1329
TauI GCSGC 1 cut(s) 1935
TfiI GAWTC 4 cut(s) 482, 1660, 1754, 1999
Tru1I TTAA 5 cut(s) 581, 1713, 1938, 2178, 2198
Tru9I TTAA 5 cut(s) 581, 1713, 1938, 2178, 2198
TscAI CASTG 6 cut(s) 691, 726, 874, 1551, 1764, 2447
TseFI GTSAC 3 cut(s) 1104, 2444, 2689
TseI GCWGC 8 cut(s) 167, 328, 982, 1317, 1808, 1964, 2244, 2927
Tsp45I GTSAC 3 cut(s) 1104, 2444, 2689
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 6 cut(s) 691, 726, 874, 1551, 1764, 2447
Van91I CCANNNNNTGG 1 cut(s) 2621
Vha464I CTTAAG 1 cut(s) 2197
VpaK11BI GGWCC 2 cut(s) 657, 1045
XapI RAATTY 9 cut(s) 208, 621, 693, 1051, 1243, 1404, 2005, 2161, 3000
XbaI TCTAGA 2 cut(s) 892, 2810
XceI RCATGY 2 cut(s) 1820, 2719
XhoI CTCGAG 1 cut(s) 2681
XmiI GTMKAC 2 cut(s) 2097, 3011
XmnI GAANNNNTTC 4 cut(s) 1081, 1473, 1998, 3004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.