Prupe.1G030200_v2.0.a1

DNA-directed RNA polymerase V subunit 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0003344
Physical Location & Seq
Reverse (-)
2157321 .. 2159501
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G030200.7

Sequence Viewer

Length: 636 bp
ATGCATCTCTTAAGAATGGATTTAGAGGTCTTTTATAAGAGTCAGATGAGAGAATTTTTTGAAGTAGATCTGAACATGTTTTATGAAGTGGAACTGCTCAGGGAAGTGGCTGTCCTTGCTGAAAATCTAGACAGGGACAAACTTGTTTCTTCGAGGTTCATCGTAACACGGCTTTTGGAGGGTTTGTTAAGCGAGAAGGCAGACGAGGATCTTGGATACTTTCTTGCAGTCACTGGCATAAAGAGGATCGGCAAAGGAGAAGTTGTACACAACTCAGGAGATGTGTTCTTCCCTGTAGTTTTCAATTGTCGGATGTTCCTGCCTCGCAAGGGAGAGATCTTGGAAGGAGTCGTTGATCACGTACACAGACTTGGGGTTTTCTTGAGATGTGGACCTGTTAAATATGTTTTTCTTTCTGCTAGAAAAATGCCAAATTACCGCTATGTTGTTGGGGAAAAACCCGTCTTCTTGCATGATGACCTTGCGAGGATAGAGAAGGATGTTGTGGTTCGCTTCGAGGTGTTTGGAGTCAGATGGATGAGGAGGGAAGATATAACAAAGGAGTTTATGATGCTTGCCACTTTACAGGGTGATTTGCTTGGTCCCGTTACTGGTCCTGATGGACTGGATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000291 GO:0000428 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0003727 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006366 GO:0006367 GO:0006401 GO:0006402 GO:0006417 GO:0006446 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0016070 GO:0016071 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031369 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032774 GO:0032991 GO:0034248 GO:0034250 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045727 GO:0045935 GO:0045948 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0055029 GO:0060211 GO:0060213 GO:0060255 GO:0061013 GO:0061014 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1900151 GO:1900153 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1902494 GO:1903311 GO:1903313 GO:1990234 GO:1990904 GO:2000112
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHS2_Rpb7-N

Protein Analysis

212

Amino Acids

24.45

Weight (kDa)

5.8

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 36
AciI CCGC 1 cut(s) 439
AclWI GGATC 2 cut(s) 216, 254
AcsI RAATTY 1 cut(s) 53
AfaI GTAC 2 cut(s) 267, 363
AfiI CCNNNNNNNGG 2 cut(s) 329, 611
AflII CTTAAG 1 cut(s) 10
AflIII ACRYGT 1 cut(s) 75
AgsI TTSAA 2 cut(s) 62, 304
AlwI GGATC 2 cut(s) 216, 254
AlwNI CAGNNNCTG 1 cut(s) 233
ApoI RAATTY 1 cut(s) 53
AspS9I GGNCC 3 cut(s) 392, 602, 614
AsuHPI GGTGA 1 cut(s) 602
AvaII GGWCC 3 cut(s) 392, 602, 614
BbsI GAAGAC 1 cut(s) 457
BccI CCATC 2 cut(s) 528, 614
BceAI ACGGC 1 cut(s) 185
BciVI GTATCC 1 cut(s) 209
BclI TGATCA 1 cut(s) 355
BfaI CTAG 2 cut(s) 128, 420
BfmI CTRYAG 1 cut(s) 294
BfrI CTTAAG 1 cut(s) 10
BfuI GTATCC 1 cut(s) 209
BglII AGATCT 2 cut(s) 67, 336
Bme18I GGWCC 3 cut(s) 392, 602, 614
BmgT120I GGNCC 3 cut(s) 392, 602, 614
BmiI GGNNCC 1 cut(s) 604
BmsI GCATC 2 cut(s) 13, 561
BpiI GAAGAC 1 cut(s) 457
Bpu10I CCTNAGC 1 cut(s) 98
BpuEI CTTGAG 1 cut(s) 403
BsaAI YACGTR 1 cut(s) 361
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 249, 279
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 611
Bse1I ACTGG 3 cut(s) 238, 616, 630
BseGI GGATG 3 cut(s) 318, 505, 543
BseLI CCNNNNNNNGG 2 cut(s) 329, 611
BseMII CTCAG 2 cut(s) 112, 288
BseNI ACTGG 3 cut(s) 238, 616, 630
BseRI GAGGAG 1 cut(s) 556
BslFI GGGAC 2 cut(s) 149, 588
BslI CCNNNNNNNGG 2 cut(s) 329, 611
BsmFI GGGAC 2 cut(s) 149, 588
Bsp1407I TGTACA 1 cut(s) 265
Bsp143I GATC 5 cut(s) 67, 208, 246, 336, 355
BspACI CCGC 1 cut(s) 439
BspCNI CTCAG 2 cut(s) 111, 287
BspLI GGNNCC 1 cut(s) 604
BspPI GGATC 2 cut(s) 216, 254
BspTI CTTAAG 1 cut(s) 10
BsrGI TGTACA 1 cut(s) 265
BsrI ACTGG 3 cut(s) 238, 616, 630
BssMI GATC 5 cut(s) 67, 208, 246, 336, 355
BstAFI CTTAAG 1 cut(s) 10
BstAUI TGTACA 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 361
BstC8I GCNNGC 1 cut(s) 576
BstDEI CTNAG 2 cut(s) 98, 274
BstF5I GGATG 3 cut(s) 318, 505, 543
BstKTI GATC 5 cut(s) 70, 211, 249, 339, 358
BstMBI GATC 5 cut(s) 67, 208, 246, 336, 355
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNSI RCATGY 1 cut(s) 79
BstSFI CTRYAG 1 cut(s) 294
BstV2I GAAGAC 1 cut(s) 457
BstX2I RGATCY 3 cut(s) 67, 208, 336
BstYI RGATCY 3 cut(s) 67, 208, 336
BsuI GTATCC 1 cut(s) 209
BtsCI GGATG 3 cut(s) 318, 505, 543
BtsIMutI CAGTG 1 cut(s) 231
Cac8I GCNNGC 1 cut(s) 576
CaiI CAGNNNCTG 1 cut(s) 233
Cfr13I GGNCC 3 cut(s) 392, 602, 614
Csp6I GTAC 2 cut(s) 266, 362
CviAII CATG 2 cut(s) 76, 473
CviJI RGCY 2 cut(s) 110, 172
CviKI_1 RGCY 2 cut(s) 110, 172
CviQI GTAC 2 cut(s) 266, 362
DdeI CTNAG 2 cut(s) 98, 274
DpnI GATC 5 cut(s) 69, 210, 248, 338, 357
DpnII GATC 5 cut(s) 67, 208, 246, 336, 355
Eco47I GGWCC 3 cut(s) 392, 602, 614
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 79, 476
FaiI YATR 9 cut(s) 36, 77, 84, 239, 405, 444, 474, 554, 569
FaqI GGGAC 2 cut(s) 149, 588
FatI CATG 2 cut(s) 75, 472
FbaI TGATCA 1 cut(s) 355
FokI GGATG 3 cut(s) 325, 512, 550
FspBI CTAG 2 cut(s) 128, 420
Hin1II CATG 2 cut(s) 79, 476
HinfI GANTC 3 cut(s) 40, 348, 528
HphI GGTGA 1 cut(s) 602
Hpy166II GTNNAC 3 cut(s) 268, 364, 392
Hpy188I TCNGA 4 cut(s) 45, 72, 312, 533
Hpy188III TCNNGA 4 cut(s) 128, 276, 382, 617
Hpy8I GTNNAC 3 cut(s) 268, 364, 392
HpyAV CCTTC 3 cut(s) 190, 338, 490
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 3 cut(s) 4, 227, 472
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 2 cut(s) 98, 274
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 2 cut(s) 79, 476
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 5 cut(s) 67, 208, 246, 336, 355
LweI GCATC 2 cut(s) 13, 561
MaeI CTAG 2 cut(s) 128, 420
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 3 cut(s) 163, 229, 607
MalI GATC 5 cut(s) 69, 210, 248, 338, 357
MboI GATC 5 cut(s) 67, 208, 246, 336, 355
MboII GAAGA 4 cut(s) 141, 280, 457, 560
MfeI CAATTG 1 cut(s) 304
MflI RGATCY 3 cut(s) 67, 208, 336
MluCI AATT 3 cut(s) 53, 304, 433
MlyI GAGTC 3 cut(s) 49, 357, 537
MmeI TCCRAC 1 cut(s) 290
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 11, 188, 399
MspCI CTTAAG 1 cut(s) 10
MunI CAATTG 1 cut(s) 304
MwoI GCNNNNNNNGC 1 cut(s) 116
NdeII GATC 5 cut(s) 67, 208, 246, 336, 355
NlaIII CATG 2 cut(s) 79, 476
NlaIV GGNNCC 1 cut(s) 604
NmuCI GTSAC 1 cut(s) 229
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 79
PciI ACATGT 1 cut(s) 75
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PleI GAGTC 3 cut(s) 48, 356, 536
PpsI GAGTC 3 cut(s) 48, 356, 536
Ppu21I YACGTR 1 cut(s) 361
PscI ACATGT 1 cut(s) 75
PsiI TTATAA 1 cut(s) 36
PspN4I GGNNCC 1 cut(s) 604
PspPI GGNCC 3 cut(s) 392, 602, 614
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 3 cut(s) 67, 208, 336
RsaI GTAC 2 cut(s) 267, 363
RsaNI GTAC 2 cut(s) 266, 362
SaqAI TTAA 3 cut(s) 11, 188, 399
Sau3AI GATC 5 cut(s) 67, 208, 246, 336, 355
Sau96I GGNCC 3 cut(s) 392, 602, 614
SchI GAGTC 3 cut(s) 49, 357, 537
SetI ASST 6 cut(s) 30, 158, 363, 397, 483, 522
SfaNI GCATC 2 cut(s) 13, 561
SfcI CTRYAG 1 cut(s) 294
SinI GGWCC 3 cut(s) 392, 602, 614
SmlI CTYRAG 2 cut(s) 10, 382
SmoI CTYRAG 2 cut(s) 10, 382
Sse9I AATT 3 cut(s) 53, 304, 433
SsiI CCGC 1 cut(s) 439
SspMI CTAG 2 cut(s) 128, 420
TaiI ACGT 1 cut(s) 363
TaqI TCGA 2 cut(s) 152, 516
TasI AATT 3 cut(s) 53, 304, 433
TatI WGTACW 1 cut(s) 265
Tru1I TTAA 3 cut(s) 11, 188, 399
Tru9I TTAA 3 cut(s) 11, 188, 399
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 2 cut(s) 99, 148
TspRI CASTG 1 cut(s) 238
Vha464I CTTAAG 1 cut(s) 10
VpaK11BI GGWCC 3 cut(s) 392, 602, 614
XapI RAATTY 1 cut(s) 53
XbaI TCTAGA 1 cut(s) 127
XceI RCATGY 1 cut(s) 79
XspI CTAG 2 cut(s) 128, 420
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.