Prupe.1G083800_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014825
Physical Location & Seq
Reverse (-)
6231175 .. 6238147
6973 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G083800.6

Sequence Viewer

Length: 741 bp
ATGATCGAGAACCCAGCCAATCTTTCACTAATCTCTCTTGAAGAGGACAAGGAGATAGCCAAGCCAAGGCTCACCCGCCTTTTGAGTCAAACCAGTGGCAGCAGCATACATGATGCATTACAGGAAGCATTGCAAAAACTAGGATTTTACTCGGGTGAGGAAGACATGGAGTTTTCCAGCTTCTCAAGCGGGACCGAACGTGCTGTCAAGACTTGGCAAGCCTCCTTAGACGCCCCTCAAGATGGCATAGTGACTGCAGAACTTGAACAATTATATACGGTGGCACAAATTGAGGGTCCGGATGAGTACTCTGACTGCTACACACAAGACATGCATCCTGGAAATATCCCTGTTCGGCTGGCTCAGAGCAAGTCTTCCCGGAAGCGACTCTTCAAAACAAAGCCTCATGTCATTTTCCTTGACTTAGGCACGACTGCTGAACTCTCTAAAAGTGATAAGAGTGATTTCAAGGCTGTTGCTCTTCGTGATGGGCGTACTGCTGCTGAGTGCACACTCAAACTATCCAAGCAACACAAGTGCCCAGATCCAAAGGCTTTCATTGAGCAAGTCGAGGAGGCATTTGCTTTCTGGGGTACTCCAGAAGCTGATCTGGTCCATCCTGCCGAGTATATGCAGCAGTTACTCGAGAAAGTTAGGCGTCATAGAGTCAATGTCTGTACTTTCATGGTAACCACTTTGGCTCTTGAGGTAATTTCTTTTTCCTTTATCTACTGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

247

Amino Acids

27.59

Weight (kDa)

5.24

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 2 cut(s) 76, 189
AclWI GGATC 1 cut(s) 539
AcyI GRCGYC 2 cut(s) 231, 658
AfaI GTAC 4 cut(s) 308, 496, 595, 679
AfiI CCNNNNNNNGG 2 cut(s) 66, 242
AgsI TTSAA 4 cut(s) 41, 266, 394, 469
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 3 cut(s) 180, 605, 738
AluI AGCT 3 cut(s) 180, 605, 738
Alw21I GWGCWC 1 cut(s) 512
Alw44I GTGCAC 1 cut(s) 508
AlwI GGATC 1 cut(s) 539
AlwNI CAGNNNCTG 1 cut(s) 605
Ama87I CYCGRG 2 cut(s) 151, 644
Aor13HI TCCGGA 1 cut(s) 298
ApaLI GTGCAC 1 cut(s) 508
ApeKI GCWGC 5 cut(s) 99, 102, 500, 634, 735
ArsI GACNNNNNNTTYG 4 cut(s) 189, 221, 280, 312
AspS9I GGNCC 3 cut(s) 192, 296, 613
AsuC2I CCSGG 1 cut(s) 379
AsuHPI GGTGA 2 cut(s) 64, 167
AvaI CYCGRG 2 cut(s) 151, 644
AvaII GGWCC 3 cut(s) 192, 296, 613
BaeGI GKGCMC 2 cut(s) 512, 542
BbsI GAAGAC 2 cut(s) 168, 366
Bbv12I GWGCWC 1 cut(s) 512
BbvI GCAGC 4 cut(s) 111, 114, 487, 646
BccI CCATC 3 cut(s) 236, 482, 624
BciT130I CCWGG 1 cut(s) 339
BcnI CCSGG 1 cut(s) 379
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 2 cut(s) 255, 733
BisI GCNGC 5 cut(s) 100, 103, 501, 635, 736
BlsI GCNGC 5 cut(s) 101, 104, 502, 636, 737
BmcAI AGTACT 1 cut(s) 308
Bme1390I CCNGG 2 cut(s) 339, 379
Bme18I GGWCC 3 cut(s) 192, 296, 613
BmeT110I CYCGRG 2 cut(s) 151, 644
BmgT120I GGNCC 3 cut(s) 192, 296, 613
BmiI GGNNCC 2 cut(s) 193, 297
BmrFI CCNGG 2 cut(s) 339, 379
BmsI GCATC 2 cut(s) 103, 343
BpiI GAAGAC 2 cut(s) 168, 366
BpmI CTGGAG 1 cut(s) 582
BpuEI CTTGAG 3 cut(s) 169, 222, 725
BpuMI CCSGG 1 cut(s) 379
BsaHI GRCGYC 2 cut(s) 231, 658
BsaJI CCNNGG 1 cut(s) 65
BsaWI WCCGGW 1 cut(s) 298
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 242
Bse1I ACTGG 1 cut(s) 93
Bse3DI GCAATG 1 cut(s) 128
BseAI TCCGGA 1 cut(s) 298
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 65
BseGI GGATG 3 cut(s) 307, 334, 616
BseLI CCNNNNNNNGG 2 cut(s) 66, 242
BseMI GCAATG 1 cut(s) 128
BseMII CTCAG 2 cut(s) 377, 495
BseNI ACTGG 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 587
BseSI GKGCMC 2 cut(s) 512, 542
BseXI GCAGC 4 cut(s) 111, 114, 487, 646
BseYI CCCAGC 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 512
BsiHKCI CYCGRG 2 cut(s) 151, 644
BsiSI CCGG 2 cut(s) 299, 379
BslFI GGGAC 1 cut(s) 205
BslI CCNNNNNNNGG 2 cut(s) 66, 242
BsmFI GGGAC 1 cut(s) 205
BsoBI CYCGRG 2 cut(s) 151, 644
Bsp1286I GDGCHC 2 cut(s) 512, 542
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 3 cut(s) 3, 544, 607
BspACI CCGC 2 cut(s) 76, 189
BspCNI CTCAG 2 cut(s) 376, 496
BspEI TCCGGA 1 cut(s) 298
BspLI GGNNCC 2 cut(s) 193, 297
BspMAI CTGCAG 2 cut(s) 259, 737
BspPI GGATC 1 cut(s) 539
BspQI GCTCTTC 1 cut(s) 486
BsrDI GCAATG 1 cut(s) 128
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 65
BssMI GATC 3 cut(s) 3, 544, 607
BssNI GRCGYC 2 cut(s) 231, 658
BssT1I CCWWGG 1 cut(s) 65
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 3 cut(s) 36, 395, 486
BstACI GRCGYC 2 cut(s) 231, 658
BstC8I GCNNGC 2 cut(s) 219, 360
BstDEI CTNAG 4 cut(s) 226, 363, 424, 504
BstEII GGTNACC 1 cut(s) 688
BstF5I GGATG 3 cut(s) 307, 334, 616
BstKTI GATC 3 cut(s) 6, 547, 610
BstMBI GATC 3 cut(s) 3, 544, 607
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstNI CCWGG 1 cut(s) 339
BstNSI RCATGY 1 cut(s) 334
BstPI GGTNACC 1 cut(s) 688
BstSCI CCNGG 2 cut(s) 337, 377
BstSFI CTRYAG 2 cut(s) 255, 733
BstSLI GKGCMC 2 cut(s) 512, 542
BstV1I GCAGC 4 cut(s) 111, 114, 487, 646
BstV2I GAAGAC 2 cut(s) 168, 366
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
BtsCI GGATG 3 cut(s) 307, 334, 616
BtsIMutI CAGTG 1 cut(s) 100
Cac8I GCNNGC 2 cut(s) 219, 360
CaiI CAGNNNCTG 1 cut(s) 605
Cfr13I GGNCC 3 cut(s) 192, 296, 613
CseI GACGC 2 cut(s) 239, 647
Csp6I GTAC 4 cut(s) 307, 495, 594, 678
CviAII CATG 5 cut(s) 110, 166, 331, 407, 685
CviQI GTAC 4 cut(s) 307, 495, 594, 678
DdeI CTNAG 4 cut(s) 226, 363, 424, 504
DpnI GATC 3 cut(s) 5, 546, 609
DpnII GATC 3 cut(s) 3, 544, 607
Eam1104I CTCTTC 3 cut(s) 36, 395, 486
EarI CTCTTC 3 cut(s) 36, 395, 486
Eco130I CCWWGG 1 cut(s) 65
Eco47I GGWCC 3 cut(s) 192, 296, 613
Eco88I CYCGRG 2 cut(s) 151, 644
Eco91I GGTNACC 1 cut(s) 688
EcoO65I GGTNACC 1 cut(s) 688
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 65
EcoT22I ATGCAT 2 cut(s) 118, 336
ErhI CCWWGG 1 cut(s) 65
FaeI CATG 5 cut(s) 113, 169, 334, 410, 688
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FaqI GGGAC 1 cut(s) 205
FatI CATG 5 cut(s) 109, 165, 330, 406, 684
FauI CCCGC 2 cut(s) 83, 182
Fnu4HI GCNGC 5 cut(s) 100, 103, 501, 635, 736
FokI GGATG 3 cut(s) 314, 321, 603
Fsp4HI GCNGC 5 cut(s) 100, 103, 501, 635, 736
FspBI CTAG 1 cut(s) 140
GluI GCNGC 5 cut(s) 100, 103, 501, 635, 736
GsaI CCCAGC 1 cut(s) 17
GsuI CTGGAG 1 cut(s) 582
HapII CCGG 2 cut(s) 299, 379
HgaI GACGC 2 cut(s) 239, 647
Hin1I GRCGYC 2 cut(s) 231, 658
Hin1II CATG 5 cut(s) 113, 169, 334, 410, 688
HinfI GANTC 3 cut(s) 85, 387, 666
HpaII CCGG 2 cut(s) 299, 379
HphI GGTGA 2 cut(s) 64, 167
Hpy166II GTNNAC 1 cut(s) 510
Hpy188I TCNGA 2 cut(s) 313, 366
Hpy188III TCNNGA 9 cut(s) 7, 38, 208, 239, 299, 485, 599, 646, 704
Hpy8I GTNNAC 1 cut(s) 510
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 1 cut(s) 199
HpyCH4V TGCA 7 cut(s) 116, 133, 257, 334, 510, 634, 735
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 4 cut(s) 226, 363, 424, 504
HpySE526I ACGT 1 cut(s) 199
Hsp92I GRCGYC 2 cut(s) 231, 658
Hsp92II CATG 5 cut(s) 113, 169, 334, 410, 688
Kpn2I TCCGGA 1 cut(s) 298
Kzo9I GATC 3 cut(s) 3, 544, 607
LguI GCTCTTC 1 cut(s) 486
Lsp1109I GCAGC 4 cut(s) 111, 114, 487, 646
LweI GCATC 2 cut(s) 103, 343
MaeI CTAG 1 cut(s) 140
MaeII ACGT 1 cut(s) 199
MaeIII GTNAC 3 cut(s) 250, 639, 688
MalI GATC 3 cut(s) 5, 546, 609
MboI GATC 3 cut(s) 3, 544, 607
MboII GAAGA 5 cut(s) 53, 173, 366, 382, 473
MflI RGATCY 1 cut(s) 544
MhlI GDGCHC 2 cut(s) 512, 542
MluCI AATT 3 cut(s) 269, 288, 711
MlyI GAGTC 3 cut(s) 94, 381, 675
MnlI CCTC 9 cut(s) 37, 151, 232, 246, 286, 414, 565, 568, 700
Mph1103I ATGCAT 2 cut(s) 118, 336
MroI TCCGGA 1 cut(s) 298
MspA1I CMGCKG 1 cut(s) 738
MspI CCGG 2 cut(s) 299, 379
MspR9I CCNGG 2 cut(s) 339, 379
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 186
NciI CCSGG 1 cut(s) 379
NdeII GATC 3 cut(s) 3, 544, 607
NlaIII CATG 5 cut(s) 113, 169, 334, 410, 688
NlaIV GGNNCC 2 cut(s) 193, 297
NmeAIII GCCGAG 1 cut(s) 649
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 2 cut(s) 118, 336
NspI RCATGY 1 cut(s) 334
PaeR7I CTCGAG 1 cut(s) 644
PciSI GCTCTTC 1 cut(s) 486
PcsI WCGNNNNNNNCGW 1 cut(s) 490
PfoI TCCNGGA 2 cut(s) 337, 377
PkrI GCNGC 5 cut(s) 101, 104, 502, 636, 737
PleI GAGTC 3 cut(s) 93, 381, 674
PpsI GAGTC 3 cut(s) 93, 381, 674
Psp6I CCWGG 1 cut(s) 337
PspEI GGTNACC 1 cut(s) 688
PspFI CCCAGC 1 cut(s) 13
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 2 cut(s) 193, 297
PspPI GGNCC 3 cut(s) 192, 296, 613
PstI CTGCAG 2 cut(s) 259, 737
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 1 cut(s) 544
PvuII CAGCTG 1 cut(s) 738
RsaI GTAC 4 cut(s) 308, 496, 595, 679
RsaNI GTAC 4 cut(s) 307, 495, 594, 678
SapI GCTCTTC 1 cut(s) 486
SatI GCNGC 5 cut(s) 100, 103, 501, 635, 736
Sau3AI GATC 3 cut(s) 3, 544, 607
Sau96I GGNCC 3 cut(s) 192, 296, 613
ScaI AGTACT 1 cut(s) 308
SchI GAGTC 3 cut(s) 94, 381, 675
ScrFI CCNGG 2 cut(s) 339, 379
SduI GDGCHC 2 cut(s) 512, 542
SetI ASST 5 cut(s) 182, 202, 607, 711, 740
SfaNI GCATC 2 cut(s) 103, 343
SfcI CTRYAG 2 cut(s) 255, 733
Sfr274I CTCGAG 1 cut(s) 644
SinI GGWCC 3 cut(s) 192, 296, 613
SlaI CTCGAG 1 cut(s) 644
SmlI CTYRAG 4 cut(s) 184, 237, 644, 704
SmoI CTYRAG 4 cut(s) 184, 237, 644, 704
Sse9I AATT 3 cut(s) 269, 288, 711
SsiI CCGC 2 cut(s) 76, 189
SspMI CTAG 1 cut(s) 140
StyD4I CCNGG 2 cut(s) 337, 377
StyI CCWWGG 1 cut(s) 65
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 1 cut(s) 202
TaqI TCGA 3 cut(s) 6, 570, 645
TaqII GACCGA 1 cut(s) 209
TasI AATT 3 cut(s) 269, 288, 711
TatI WGTACW 2 cut(s) 306, 677
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 5 cut(s) 99, 102, 500, 634, 735
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 547, 673
TspRI CASTG 1 cut(s) 100
VneI GTGCAC 1 cut(s) 508
VpaK11BI GGWCC 3 cut(s) 192, 296, 613
XceI RCATGY 1 cut(s) 334
XhoI CTCGAG 1 cut(s) 644
XspI CTAG 1 cut(s) 140
ZrmI AGTACT 1 cut(s) 308
Zsp2I ATGCAT 2 cut(s) 118, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.