Prupe.3G103700_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0015663
Physical Location & Seq
Reverse (-)
8155785 .. 8160858
5074 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G103700.1

Sequence Viewer

Length: 1572 bp
ATGGTTCAAATAATGAGTCCGCGAAAGGTTGCGGTGAAGTTGGATGCCGATGAGTCGTTCGACACCCAACTCGGTCCAATTCACAAGCGGCTCAAACTCGACTCGGTTCCACAGTTGGGTTTGGGGCCTGGCAAGTTTGAAGTTCCTCCTTCGCTGTACCATCCCCTGGATGAACCGAGCCCGCTCGGTTTGCATCTAAAAAAAAGTCCATCTTTTTTGGATTTGATCCAAATGAAGCTATCTGAAGAAAACTCTGTTAAATTGGCTACTCTAGGCAAGAGAGGTCACAAGGGAACTACCGCTTCCGGTGCTTCGGATAAGCTCAAGGCTATGAATTTCCCGGCTTCCATTCTAAGGATTGGGACTTGGGAGTATAAGTCAAGATATGAGGGAGATCTAGTGGCAAAGTGTTATTTTGCAAAGCATAAACTTGTGTGGGAAGTCCTTGATGGTAGTCTAAAGAACAAGATGGAAATTCAGTGGTCAGATGTTGTGGCTCTCAAGGCAAATTACCCAGATGATGGACCAGGGACTTTGGATGTTGTGCTTGCTAGACCGCCTCTTTTCTTTAGAGAGACCAATCCACAACCTAGAAAACATACCTTGTGGCAGGCAACATCAGATTTTACTGCTGGGCAGGCCACTTTAAACAGGCGCCATTTTCTGCAGTGCCCACAAGGCTTGCTTTTTGGCAAGCATTTCGAAAAGCTTATTCAGTGTGATCCTCGTCTCAACTTCTTAAGCCAACAGCCAGAAATTGTGTTAGAATCTCCATTTTTCGATGCTAGAGTTACAGTATCAGGTGACCCTGATGAATATGGACGTCGGTTTGGTCCAAAAAATGAGGAGGGGCATGCTATATTTGGTTTGCAGGATTCAGCATCACCTTCTGGAACTCACTCGCCACCGTGTAAAGATGAACAAGATTTTATTGGTAGAGGTCCTGAAAATTATTCCCAGGAAATTTCTTCTCCTAGCTCAGTGATGGACACCCCTGCAATTGATAAAATTGGGAGCTTTGGAGCTGAAACCTCAAAGACGCCCAGCAATTTTAATCAAATGAAAGTTCCTGGACTTCCCATGTCAATGTCAATGAGCGATTTTGTAAGCCACATTGGACAATGCATTTCTGGAAAGATATCTGGCAAGGAGCAACCTAGCAGAGATGTTTTGGAGGAGATTACTCAATACTTGTTCAATGACTCGCAATTCACCTTACCCTCAGATGAGCAATACGTCATGTCAAGGGTCAATTCCCTATATTGCCTTTTGCAGAAGGATCCTTCTACAACACTAAACTTGCAAGCCAAAACTGATGACTGCTTTGATGTAAATGATGATGGGAAAACAGCTGAAACTAACTCCGGTTCTGCATCAGCTTATGAGAGTAAGGTTTCAGATGGTTTTCCTGCACCTAAGGACGAGTCCAATGAAGGTAAGTCCAGTGCTGTTCCAAATGATGATGACAATAAGCAGGAACTGACAATGCCAAGAAGGGACTCATTTGGGGAGCTTCTTCTAAATATTCCTAGGATAGCATCCCTTCCTCAGTTTCTTTTTAACTGGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

524

Amino Acids

57.96

Weight (kDa)

5.5

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015663)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28260 FvH4_6g28260
malus_domestica MD09G1227700.v1.1 MD17G1226500.v1.1
prunus_persica Prupe.3G103700_v2.0.a1
pyrus_communis pycom09g14710 pycom17g22990
rosa_chinensis RchiOBHm_Chr2g0132301
rosa_laevigata RLG00000019244
rosa_roxburghii Rroxscaffold_2G00112180
rosa_rugosa Rorug02G0303500
rosa_samantha Rh2AG356500 Rh2BG363000 Rh2CG340000 Rh2DG379800
rosa_wichuraiana Rw2G028990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 826
AccB1I GGYRCC 1 cut(s) 654
AccB7I CCANNNNNTGG 2 cut(s) 166, 521
AccBSI CCGCTC 1 cut(s) 184
AccII CGCG 1 cut(s) 22
AciI CCGC 6 cut(s) 20, 32, 88, 182, 300, 557
AclWI GGATC 4 cut(s) 220, 716, 1274, 1287
AcsI RAATTY 3 cut(s) 334, 474, 963
AcuI CTGAAG 1 cut(s) 264
AcyI GRCGYC 3 cut(s) 655, 823, 1040
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 4 cut(s) 116, 166, 354, 521
AflII CTTAAG 1 cut(s) 739
AgsI TTSAA 3 cut(s) 8, 140, 1198
AjnI CCWGG 5 cut(s) 127, 165, 526, 957, 1069
AluBI AGCT 9 cut(s) 238, 322, 709, 978, 1017, 1025, 1352, 1379, 1513
AluI AGCT 9 cut(s) 238, 322, 709, 978, 1017, 1025, 1352, 1379, 1513
Alw26I GTCTC 2 cut(s) 569, 734
AlwI GGATC 4 cut(s) 220, 716, 1274, 1287
AlwNI CAGNNNCTG 1 cut(s) 1480
AoxI GGCC 2 cut(s) 125, 639
ApoI RAATTY 3 cut(s) 334, 474, 963
ArsI GACNNNNNNTTYG 1 cut(s) 33
AspA2I CCTAGG 1 cut(s) 1529
AspLEI GCGC 1 cut(s) 657
AspS9I GGNCC 5 cut(s) 74, 125, 524, 833, 941
AsuC2I CCSGG 1 cut(s) 341
AsuHPI GGTGA 4 cut(s) 46, 815, 876, 1204
AsuII TTCGAA 1 cut(s) 702
AvaII GGWCC 4 cut(s) 74, 524, 833, 941
AvrII CCTAGG 1 cut(s) 1529
AxyI CCTNAGG 1 cut(s) 1416
BaeGI GKGCMC 1 cut(s) 674
BamHI GGATCC 1 cut(s) 1279
BanI GGYRCC 1 cut(s) 654
BanII GRGCYC 1 cut(s) 182
BccI CCATC 8 cut(s) 168, 217, 443, 463, 515, 979, 1334, 1394
BcgI CGANNNNNNTGC 2 cut(s) 682, 716
BciT130I CCWGG 5 cut(s) 129, 167, 528, 959, 1071
BcnI CCSGG 1 cut(s) 341
BcoDI GTCTC 2 cut(s) 569, 734
BfaI CTAG 8 cut(s) 272, 398, 552, 591, 786, 975, 1158, 1530
BfmI CTRYAG 1 cut(s) 665
BfoI RGCGCY 1 cut(s) 658
BfrI CTTAAG 1 cut(s) 739
BglI GCCNNNNNGGC 1 cut(s) 678
BglII AGATCT 1 cut(s) 394
BisI GCNGC 1 cut(s) 89
BlnI CCTAGG 1 cut(s) 1529
BlsI GCNGC 1 cut(s) 90
Bme1390I CCNGG 6 cut(s) 129, 167, 341, 528, 959, 1071
Bme18I GGWCC 4 cut(s) 74, 524, 833, 941
BmgT120I GGNCC 5 cut(s) 74, 125, 524, 833, 941
BmiI GGNNCC 4 cut(s) 108, 126, 656, 1281
BmrFI CCNGG 6 cut(s) 129, 167, 341, 528, 959, 1071
BmsI GCATC 6 cut(s) 34, 202, 772, 890, 1382, 1547
Bpu14I TTCGAA 1 cut(s) 702
BpuEI CTTGAG 2 cut(s) 308, 485
BpuMI CCSGG 1 cut(s) 341
BsaHI GRCGYC 3 cut(s) 655, 823, 1040
BsaI GGTCTC 1 cut(s) 569
BsaJI CCNNGG 4 cut(s) 165, 527, 957, 1529
BsaWI WCCGGW 2 cut(s) 305, 1364
BsaXI ACNNNNNCTCC 4 cut(s) 362, 384, 392, 414
Bsc4I CCNNNNNNNGG 4 cut(s) 116, 166, 354, 521
Bse1I ACTGG 2 cut(s) 1443, 1568
Bse21I CCTNAGG 1 cut(s) 1416
BseBI CCWGG 5 cut(s) 129, 167, 528, 959, 1071
BseDI CCNNGG 4 cut(s) 165, 527, 957, 1529
BseGI GGATG 5 cut(s) 49, 160, 175, 544, 1538
BseLI CCNNNNNNNGG 4 cut(s) 116, 166, 354, 521
BseMII CTCAG 3 cut(s) 993, 1236, 1562
BseNI ACTGG 2 cut(s) 1443, 1568
BseRI GAGGAG 2 cut(s) 860, 1190
BseSI GKGCMC 1 cut(s) 674
BseYI CCCAGC 2 cut(s) 632, 1043
BsgI GTGCAG 1 cut(s) 1395
Bsh1236I CGCG 1 cut(s) 22
BshFI GGCC 2 cut(s) 127, 641
BshNI GGYRCC 1 cut(s) 654
BsiSI CCGG 3 cut(s) 306, 341, 1365
BslFI GGGAC 3 cut(s) 376, 544, 1511
BslI CCNNNNNNNGG 4 cut(s) 116, 166, 354, 521
BsmAI GTCTC 2 cut(s) 569, 734
BsmBI CGTCTC 1 cut(s) 734
BsmFI GGGAC 3 cut(s) 376, 544, 1511
BsnI GGCC 2 cut(s) 127, 641
Bso31I GGTCTC 1 cut(s) 569
Bsp119I TTCGAA 1 cut(s) 702
Bsp1286I GDGCHC 2 cut(s) 182, 674
Bsp143I GATC 4 cut(s) 225, 394, 721, 1279
BspACI CCGC 6 cut(s) 20, 32, 88, 182, 300, 557
BspANI GGCC 2 cut(s) 127, 641
BspCNI CTCAG 3 cut(s) 992, 1235, 1561
BspFNI CGCG 1 cut(s) 22
BspLI GGNNCC 4 cut(s) 108, 126, 656, 1281
BspMAI CTGCAG 1 cut(s) 669
BspPI GGATC 4 cut(s) 220, 716, 1274, 1287
BspT104I TTCGAA 1 cut(s) 702
BspT107I GGYRCC 1 cut(s) 654
BspTI CTTAAG 1 cut(s) 739
BspTNI GGTCTC 1 cut(s) 569
BsrBI CCGCTC 1 cut(s) 184
BsrI ACTGG 2 cut(s) 1443, 1568
BssECI CCNNGG 4 cut(s) 165, 527, 957, 1529
BssMI GATC 4 cut(s) 225, 394, 721, 1279
BssNI GRCGYC 3 cut(s) 655, 823, 1040
BssT1I CCWWGG 1 cut(s) 1529
Bst2UI CCWGG 5 cut(s) 129, 167, 528, 959, 1071
Bst4CI ACNGT 3 cut(s) 114, 796, 909
BstACI GRCGYC 3 cut(s) 655, 823, 1040
BstAFI CTTAAG 1 cut(s) 739
BstBI TTCGAA 1 cut(s) 702
BstC8I GCNNGC 8 cut(s) 182, 549, 612, 639, 683, 695, 855, 1305
BstDEI CTNAG 5 cut(s) 353, 979, 1222, 1416, 1548
BstEII GGTNACC 1 cut(s) 803
BstF5I GGATG 5 cut(s) 49, 160, 175, 544, 1538
BstFNI CGCG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 658
BstHHI GCGC 1 cut(s) 657
BstKTI GATC 4 cut(s) 228, 397, 724, 1282
BstMAI GTCTC 2 cut(s) 569, 734
BstMBI GATC 4 cut(s) 225, 394, 721, 1279
BstMWI GCNNNNNNNGC 5 cut(s) 190, 308, 503, 638, 678
BstNI CCWGG 5 cut(s) 129, 167, 528, 959, 1071
BstNSI RCATGY 1 cut(s) 857
BstPI GGTNACC 1 cut(s) 803
BstSCI CCNGG 6 cut(s) 127, 165, 339, 526, 957, 1069
BstSFI CTRYAG 1 cut(s) 665
BstSLI GKGCMC 1 cut(s) 674
BstUI CGCG 1 cut(s) 22
BstX2I RGATCY 2 cut(s) 394, 1279
BstYI RGATCY 2 cut(s) 394, 1279
Bsu36I CCTNAGG 1 cut(s) 1416
BsuRI GGCC 2 cut(s) 127, 641
BtsCI GGATG 5 cut(s) 49, 160, 175, 544, 1538
BtsI GCAGTG 1 cut(s) 674
BtsIMutI CAGTG 5 cut(s) 485, 674, 722, 987, 1450
Cac8I GCNNGC 8 cut(s) 182, 549, 612, 639, 683, 695, 855, 1305
CaiI CAGNNNCTG 1 cut(s) 1480
CfoI GCGC 1 cut(s) 657
Cfr13I GGNCC 5 cut(s) 74, 125, 524, 833, 941
CseI GACGC 1 cut(s) 1048
Csp6I GTAC 1 cut(s) 157
CspCI CAANNNNNGTGG 2 cut(s) 663, 698
CviAII CATG 3 cut(s) 854, 1081, 1240
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 5 cut(s) 353, 979, 1222, 1416, 1548
DinI GGCGCC 1 cut(s) 656
DpnI GATC 4 cut(s) 227, 396, 723, 1281
DpnII GATC 4 cut(s) 225, 394, 721, 1279
DraI TTTAAA 1 cut(s) 648
Eco130I CCWWGG 1 cut(s) 1529
Eco24I GRGCYC 1 cut(s) 182
Eco31I GGTCTC 1 cut(s) 569
Eco32I GATATC 1 cut(s) 1140
Eco47I GGWCC 4 cut(s) 74, 524, 833, 941
Eco57I CTGAAG 1 cut(s) 264
Eco81I CCTNAGG 1 cut(s) 1416
Eco91I GGTNACC 1 cut(s) 803
EcoO109I RGGNCCY 2 cut(s) 125, 941
EcoO65I GGTNACC 1 cut(s) 803
EcoRII CCWGG 5 cut(s) 127, 165, 526, 957, 1069
EcoRV GATATC 1 cut(s) 1140
EcoT14I CCWWGG 1 cut(s) 1529
EcoT22I ATGCAT 1 cut(s) 1127
EcoT38I GRGCYC 1 cut(s) 182
EgeI GGCGCC 1 cut(s) 656
EheI GGCGCC 1 cut(s) 656
ErhI CCWWGG 1 cut(s) 1529
Esp3I CGTCTC 1 cut(s) 734
FaeI CATG 3 cut(s) 857, 1084, 1243
FalI AAGNNNNNCTT 4 cut(s) 196, 228, 669, 701
FaqI GGGAC 3 cut(s) 376, 544, 1511
FatI CATG 3 cut(s) 853, 1080, 1239
FauI CCCGC 1 cut(s) 189
Fnu4HI GCNGC 1 cut(s) 89
FokI GGATG 5 cut(s) 56, 147, 182, 551, 1525
FriOI GRGCYC 1 cut(s) 182
Fsp4HI GCNGC 1 cut(s) 89
FspBI CTAG 8 cut(s) 272, 398, 552, 591, 786, 975, 1158, 1530
GlaI GCGC 1 cut(s) 656
GluI GCNGC 1 cut(s) 89
GsaI CCCAGC 2 cut(s) 636, 1047
HaeII RGCGCY 1 cut(s) 658
HaeIII GGCC 2 cut(s) 127, 641
HapII CCGG 3 cut(s) 306, 341, 1365
HgaI GACGC 1 cut(s) 1048
HhaI GCGC 1 cut(s) 657
Hin1I GRCGYC 3 cut(s) 655, 823, 1040
Hin1II CATG 3 cut(s) 857, 1084, 1243
Hin6I GCGC 1 cut(s) 655
HinP1I GCGC 1 cut(s) 655
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 8 cut(s) 16, 53, 101, 767, 875, 1202, 1424, 1499
HpaII CCGG 3 cut(s) 306, 341, 1365
HphI GGTGA 4 cut(s) 46, 815, 876, 1204
Hpy188I TCNGA 6 cut(s) 244, 316, 487, 622, 1225, 1399
Hpy188III TCNNGA 4 cut(s) 381, 891, 944, 1131
Hpy99I CGWCG 1 cut(s) 828
HpyAV CCTTC 7 cut(s) 159, 897, 1270, 1293, 1427, 1488, 1553
HpyCH4III ACNGT 3 cut(s) 114, 796, 909
HpyCH4IV ACGT 2 cut(s) 823, 1236
HpyF10VI GCNNNNNNNGC 5 cut(s) 190, 308, 503, 638, 678
HpyF3I CTNAG 5 cut(s) 353, 979, 1222, 1416, 1548
HpySE526I ACGT 2 cut(s) 823, 1236
Hsp92I GRCGYC 3 cut(s) 655, 823, 1040
Hsp92II CATG 3 cut(s) 857, 1084, 1243
HspAI GCGC 1 cut(s) 655
KasI GGCGCC 1 cut(s) 654
Kzo9I GATC 4 cut(s) 225, 394, 721, 1279
LmnI GCTCC 4 cut(s) 1014, 1022, 1150, 1510
LweI GCATC 6 cut(s) 34, 202, 772, 890, 1382, 1547
MaeI CTAG 8 cut(s) 272, 398, 552, 591, 786, 975, 1158, 1530
MaeII ACGT 2 cut(s) 823, 1236
MaeIII GTNAC 3 cut(s) 284, 790, 803
MalI GATC 4 cut(s) 227, 396, 723, 1281
MbiI CCGCTC 1 cut(s) 184
MboI GATC 4 cut(s) 225, 394, 721, 1279
MboII GAAGA 3 cut(s) 257, 960, 1508
MfeI CAATTG 1 cut(s) 999
MflI RGATCY 2 cut(s) 394, 1279
MhlI GDGCHC 2 cut(s) 182, 674
Mly113I GGCGCC 1 cut(s) 655
MlyI GAGTC 6 cut(s) 25, 62, 95, 1196, 1433, 1493
MmeI TCCRAC 1 cut(s) 21
Mph1103I ATGCAT 1 cut(s) 1127
MseI TTAA 5 cut(s) 258, 647, 740, 1053, 1560
MslI CAYNNNNRTG 1 cut(s) 1085
MspA1I CMGCKG 1 cut(s) 1352
MspCI CTTAAG 1 cut(s) 739
MspI CCGG 3 cut(s) 306, 341, 1365
MspR9I CCNGG 6 cut(s) 129, 167, 341, 528, 959, 1071
MunI CAATTG 1 cut(s) 999
MvaI CCWGG 5 cut(s) 129, 167, 528, 959, 1071
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 5 cut(s) 190, 308, 503, 638, 678
NarI GGCGCC 1 cut(s) 655
NciI CCSGG 1 cut(s) 341
NdeII GATC 4 cut(s) 225, 394, 721, 1279
NlaIII CATG 3 cut(s) 857, 1084, 1243
NlaIV GGNNCC 4 cut(s) 108, 126, 656, 1281
NmuCI GTSAC 2 cut(s) 284, 803
NsiI ATGCAT 1 cut(s) 1127
NspI RCATGY 1 cut(s) 857
NspV TTCGAA 1 cut(s) 702
PaeI GCATGC 1 cut(s) 857
PfeI GAWTC 2 cut(s) 767, 875
PflMI CCANNNNNTGG 2 cut(s) 166, 521
PfoI TCCNGGA 1 cut(s) 1069
PkrI GCNGC 1 cut(s) 90
PleI GAGTC 6 cut(s) 24, 61, 95, 1196, 1432, 1493
PluTI GGCGCC 1 cut(s) 658
PpsI GAGTC 6 cut(s) 24, 61, 95, 1196, 1432, 1493
PpuMI RGGWCCY 1 cut(s) 941
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 5 cut(s) 127, 165, 526, 957, 1069
PspEI GGTNACC 1 cut(s) 803
PspFI CCCAGC 2 cut(s) 632, 1043
PspGI CCWGG 5 cut(s) 127, 165, 526, 957, 1069
PspN4I GGNNCC 4 cut(s) 108, 126, 656, 1281
PspPI GGNCC 5 cut(s) 74, 125, 524, 833, 941
PspPPI RGGWCCY 1 cut(s) 941
PstI CTGCAG 1 cut(s) 669
PstNI CAGNNNCTG 1 cut(s) 1480
PsuI RGATCY 2 cut(s) 394, 1279
PvuII CAGCTG 1 cut(s) 1352
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
RseI CAYNNNNRTG 1 cut(s) 1085
SaqAI TTAA 5 cut(s) 258, 647, 740, 1053, 1560
SatI GCNGC 1 cut(s) 89
Sau3AI GATC 4 cut(s) 225, 394, 721, 1279
Sau96I GGNCC 5 cut(s) 74, 125, 524, 833, 941
SchI GAGTC 6 cut(s) 25, 62, 95, 1196, 1433, 1493
ScrFI CCNGG 6 cut(s) 129, 167, 341, 528, 959, 1071
SduI GDGCHC 2 cut(s) 182, 674
SfaNI GCATC 6 cut(s) 34, 202, 772, 890, 1382, 1547
SfcI CTRYAG 1 cut(s) 665
SfoI GGCGCC 1 cut(s) 656
SfuI TTCGAA 1 cut(s) 702
SinI GGWCC 4 cut(s) 74, 524, 833, 941
SmiMI CAYNNNNRTG 1 cut(s) 1085
SmlI CTYRAG 3 cut(s) 323, 500, 739
SmoI CTYRAG 3 cut(s) 323, 500, 739
SphI GCATGC 1 cut(s) 857
SsiI CCGC 6 cut(s) 20, 32, 88, 182, 300, 557
SspDI GGCGCC 1 cut(s) 654
SspI AATATT 1 cut(s) 1525
SspMI CTAG 8 cut(s) 272, 398, 552, 591, 786, 975, 1158, 1530
StyD4I CCNGG 6 cut(s) 127, 165, 339, 526, 957, 1069
StyI CCWWGG 1 cut(s) 1529
TaaI ACNGT 3 cut(s) 114, 796, 909
TaiI ACGT 2 cut(s) 826, 1239
TaqI TCGA 4 cut(s) 60, 99, 702, 780
TaqII GACCGA 1 cut(s) 62
TauI GCSGC 1 cut(s) 91
TfiI GAWTC 2 cut(s) 767, 875
Tru1I TTAA 5 cut(s) 258, 647, 740, 1053, 1560
Tru9I TTAA 5 cut(s) 258, 647, 740, 1053, 1560
TscAI CASTG 5 cut(s) 485, 674, 722, 987, 1450
TseFI GTSAC 2 cut(s) 284, 803
Tsp45I GTSAC 2 cut(s) 284, 803
TspDTI ATGAA 7 cut(s) 186, 248, 347, 828, 933, 1076, 1446
TspRI CASTG 5 cut(s) 485, 674, 722, 987, 1450
Van91I CCANNNNNTGG 2 cut(s) 166, 521
Vha464I CTTAAG 1 cut(s) 739
VpaK11BI GGWCC 4 cut(s) 74, 524, 833, 941
XapI RAATTY 3 cut(s) 334, 474, 963
XceI RCATGY 1 cut(s) 857
XmaJI CCTAGG 1 cut(s) 1529
XspI CTAG 8 cut(s) 272, 398, 552, 591, 786, 975, 1158, 1530
ZraI GACGTC 1 cut(s) 824
Zsp2I ATGCAT 1 cut(s) 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.