pycom09g14710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0015663
Physical Location & Seq
Reverse (-)
14446044 .. 14450352
4309 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g14710.2

Sequence Viewer

Length: 1494 bp
ATGGTTCAGCTAATGAGTCCGCGAAAGGTGACGGTGAAGTTGGACGGCGATGAGTCGTGTGGCACCCGACGCGGTGCGAGTCACAAACGGCTCAAACTCGACTCATCTCCGCAGATGCCGGGCGATGTGGATGCATGGGTGTGCCATCCGCTGGATGAGCCGAGTCCGCTCGGTTTGCAGCTCAAAAAAAGTCCATCTTTTTTGGATTTGATCCAGATGAAGCTGTCTCAAGAAAAATCTACTAAATTGGCAACCCTAGGCAAGAGAAGTCACAAGGGAGCTGCTGCTTCCGGTGCATCAGACAAGCTCAAGGCTATGAACTTTCCGGCTTCCCTTCTTAGGATTGGGACTTGGGAGTATAAGTCAAGATACGAAGGAGATTTGGTGGCGAAATGTTATTTTGCCAAGCATAAACTTGTATGGGAAGTGCTTGATGGTAGTCTTAAGAACAAGATGGAAATTCAGTGGTCAGATGTCGTGGCTATCAAGGCAAACTACCCGGATGATGGACCGGGGACTTTGGATGTAGTGCTTGCCAGACCGCCTCTTTTCTTTAGGGAGACCAATCCGCAACCTAGGAAACATACCTTGTGGCAGGCAACATCTGATTTTACTGCTGGACAAGCCACTTTGAACAGGCGGCATTTTCTGCAGTGCCCACAAGGGTTGCTCTTTGGCAAGCATTTCGAAAAGCTTATTCAATGTGATCCTCGTCTCAACTTTTTAAGCCAGCAGCCAGAAATTGTGTTAGAATCTCCGTTCTTCGATGCTAGAGCTACCATAACAGGTGGCCCTGATGATGAATATAGACATCAGTTTGGTACAAAGAATGAAGAGGGGTCTGCGATATTTGGTTTGCAGGAATCAGCATCACCTTCTGGAGCTCATTCGTCGCCATCTAAAAATGAACAAGATTTTAGTGGTAGAGCTCCTCAAAATTATTCCCAGGAAATTTCTTCACCTAGTACAGTGATGGACACTGCCAGAACCTCAAAGACGCTCGGCAAGTTCAATCAAATAAAAGTTCCTGGACTTCCTTCGTCAATGTCAGTGAGTGACTTCGTAAGCCACATTGGAAATTGCATTTCCGGAAAGGAACAACCTAGCACAGACATGTTGGAGGAGATTACGCAGTACTTGTTCAGTGACTCCCAAATCACGTTACCCTCTGATGAGCAGTCCCTCATGACGAGGGTCAATTCCCTTTGCTGCCTTTTGCAAAAGGATCCTTCTACAGAACTAAACTTGCAAGTCAGAAGCGATGAGTGGTTGAATGGAAGCAATGATTGGAAAGCAGCGGAAACCAACTCCGCTTCTGCATCAGCTCATGAGAGTAAGCCTGTAGAAGGTTTTCCTGCACCCAAGGGCGAGCCCAACGATGATTCTCATGACAAGCAGGAACTGACCATGCCGAGAAGGGATTCATTTGGGGAGCTGCTTCTTAATATTCCCAGGATAGCATCTCTTCCTCAGTTTCTTTTTAACTGGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

498

Amino Acids

55.12

Weight (kDa)

6.21

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015663)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28260 FvH4_6g28260
malus_domestica MD09G1227700.v1.1 MD17G1226500.v1.1
prunus_persica Prupe.3G103700_v2.0.a1
pyrus_communis pycom09g14710 pycom17g22990
rosa_chinensis RchiOBHm_Chr2g0132301
rosa_laevigata RLG00000019244
rosa_roxburghii Rroxscaffold_2G00112180
rosa_rugosa Rorug02G0303500
rosa_samantha Rh2AG356500 Rh2BG363000 Rh2CG340000 Rh2DG379800
rosa_wichuraiana Rw2G028990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 62
AccB7I CCANNNNNTGG 1 cut(s) 151
AccBSI CCGCTC 1 cut(s) 169
AccII CGCG 2 cut(s) 22, 72
AccIII TCCGGA 1 cut(s) 1088
AclWI GGATC 4 cut(s) 205, 701, 1220, 1233
AcsI RAATTY 2 cut(s) 459, 951
AfaI GTAC 3 cut(s) 823, 967, 1136
AfiI CCNNNNNNNGG 4 cut(s) 151, 339, 506, 1346
AflII CTTAAG 1 cut(s) 443
AflIII ACRYGT 1 cut(s) 1113
AgsI TTSAA 4 cut(s) 634, 701, 1012, 1273
AjnI CCWGG 3 cut(s) 945, 1027, 1451
AjuI GAANNNNNNNTTGG 2 cut(s) 1270, 1302
Alw21I GWGCWC 2 cut(s) 886, 931
Alw26I GTCTC 3 cut(s) 231, 554, 719
AlwI GGATC 4 cut(s) 205, 701, 1220, 1233
AlwNI CAGNNNCTG 1 cut(s) 1402
Aor13HI TCCGGA 1 cut(s) 1088
AoxI GGCC 1 cut(s) 790
ApeKI GCWGC 7 cut(s) 178, 281, 284, 733, 1209, 1295, 1435
ApoI RAATTY 2 cut(s) 459, 951
Asp700I GAANNNNTTC 2 cut(s) 1350, 1420
AspA2I CCTAGG 2 cut(s) 256, 575
AspS9I GGNCC 2 cut(s) 509, 791
AsuC2I CCSGG 3 cut(s) 120, 500, 513
AsuHPI GGTGA 4 cut(s) 40, 46, 864, 951
AsuII TTCGAA 1 cut(s) 687
AvaII GGWCC 1 cut(s) 509
AvrII CCTAGG 2 cut(s) 256, 575
BaeGI GKGCMC 1 cut(s) 659
BamHI GGATCC 1 cut(s) 1225
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 3 cut(s) 886, 931, 1374
Bbv12I GWGCWC 2 cut(s) 886, 931
BbvI GCAGC 7 cut(s) 190, 268, 271, 745, 1196, 1307, 1422
BccI CCATC 7 cut(s) 153, 202, 428, 448, 500, 904, 967
BceAI ACGGC 2 cut(s) 61, 104
BcgI CGANNNNNNTGC 4 cut(s) 113, 147, 667, 701
BciT130I CCWGG 3 cut(s) 947, 1029, 1453
BcnI CCSGG 3 cut(s) 120, 500, 513
BcoDI GTCTC 3 cut(s) 231, 554, 719
BfaI CTAG 5 cut(s) 257, 576, 771, 963, 1104
BfmI CTRYAG 3 cut(s) 650, 1233, 1341
BfrI CTTAAG 1 cut(s) 443
BisI GCNGC 8 cut(s) 179, 282, 285, 641, 734, 1210, 1296, 1436
BlnI CCTAGG 2 cut(s) 256, 575
BlsI GCNGC 8 cut(s) 180, 283, 286, 642, 735, 1211, 1297, 1437
BmcAI AGTACT 1 cut(s) 1136
Bme1390I CCNGG 6 cut(s) 120, 500, 513, 947, 1029, 1453
Bme18I GGWCC 1 cut(s) 509
BmgT120I GGNCC 2 cut(s) 509, 791
BmiI GGNNCC 2 cut(s) 64, 1227
BmrFI CCNGG 6 cut(s) 120, 500, 513, 947, 1029, 1453
BmsI GCATC 7 cut(s) 105, 121, 305, 757, 878, 1328, 1469
BoxI GACNNNNGTC 1 cut(s) 1193
BpmI CTGGAG 1 cut(s) 900
Bpu14I TTCGAA 1 cut(s) 687
BpuEI CTTGAG 2 cut(s) 213, 293
BpuMI CCSGG 3 cut(s) 120, 500, 513
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 6 cut(s) 256, 512, 575, 945, 1362, 1451
BsaWI WCCGGW 2 cut(s) 290, 1088
BsaXI ACNNNNNCTCC 4 cut(s) 347, 369, 377, 399
Bsc4I CCNNNNNNNGG 4 cut(s) 151, 339, 506, 1346
Bse1I ACTGG 1 cut(s) 1490
Bse3DI GCAATG 1 cut(s) 1288
BseAI TCCGGA 1 cut(s) 1088
BseBI CCWGG 3 cut(s) 947, 1029, 1453
BseDI CCNNGG 6 cut(s) 256, 512, 575, 945, 1362, 1451
BseGI GGATG 5 cut(s) 136, 145, 160, 508, 529
BseLI CCNNNNNNNGG 4 cut(s) 151, 339, 506, 1346
BseMI GCAATG 1 cut(s) 1288
BseMII CTCAG 1 cut(s) 1484
BseNI ACTGG 1 cut(s) 1490
BseRI GAGGAG 2 cut(s) 921, 1136
BseSI GKGCMC 1 cut(s) 659
BseXI GCAGC 7 cut(s) 190, 268, 271, 745, 1196, 1307, 1422
BsgI GTGCAG 1 cut(s) 1341
Bsh1236I CGCG 2 cut(s) 22, 72
BshFI GGCC 1 cut(s) 792
BshNI GGYRCC 1 cut(s) 62
BsiHKAI GWGCWC 2 cut(s) 886, 931
BsiSI CCGG 6 cut(s) 119, 291, 326, 500, 512, 1089
BslFI GGGAC 3 cut(s) 361, 529, 1165
BslI CCNNNNNNNGG 4 cut(s) 151, 339, 506, 1346
BsmAI GTCTC 3 cut(s) 231, 554, 719
BsmBI CGTCTC 1 cut(s) 719
BsmFI GGGAC 3 cut(s) 361, 529, 1165
BsnI GGCC 1 cut(s) 792
Bso31I GGTCTC 1 cut(s) 554
Bsp119I TTCGAA 1 cut(s) 687
Bsp1286I GDGCHC 4 cut(s) 659, 886, 931, 1374
Bsp13I TCCGGA 1 cut(s) 1088
Bsp143I GATC 3 cut(s) 210, 706, 1225
BspANI GGCC 1 cut(s) 792
BspCNI CTCAG 1 cut(s) 1483
BspEI TCCGGA 1 cut(s) 1088
BspFNI CGCG 2 cut(s) 22, 72
BspHI TCATGA 3 cut(s) 1185, 1327, 1387
BspLI GGNNCC 2 cut(s) 64, 1227
BspMAI CTGCAG 1 cut(s) 654
BspPI GGATC 4 cut(s) 205, 701, 1220, 1233
BspT104I TTCGAA 1 cut(s) 687
BspT107I GGYRCC 1 cut(s) 62
BspTI CTTAAG 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 554
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 1288
BsrI ACTGG 1 cut(s) 1490
BssECI CCNNGG 6 cut(s) 256, 512, 575, 945, 1362, 1451
BssMI GATC 3 cut(s) 210, 706, 1225
BssT1I CCWWGG 3 cut(s) 256, 575, 1362
Bst2UI CCWGG 3 cut(s) 947, 1029, 1453
Bst4CI ACNGT 2 cut(s) 34, 970
Bst6I CTCTTC 2 cut(s) 828, 1470
BstAFI CTTAAG 1 cut(s) 443
BstAPI GCANNNNNTGC 1 cut(s) 649
BstBI TTCGAA 1 cut(s) 687
BstC8I GCNNGC 5 cut(s) 534, 597, 680, 731, 1370
BstDEI CTNAG 2 cut(s) 338, 1470
BstENI CCTNNNNNAGG 1 cut(s) 1344
BstF5I GGATG 5 cut(s) 136, 145, 160, 508, 529
BstFNI CGCG 2 cut(s) 22, 72
BstKTI GATC 3 cut(s) 213, 709, 1228
BstMAI GTCTC 3 cut(s) 231, 554, 719
BstMBI GATC 3 cut(s) 210, 706, 1225
BstMWI GCNNNNNNNGC 8 cut(s) 69, 157, 166, 175, 293, 488, 623, 649
BstNI CCWGG 3 cut(s) 947, 1029, 1453
BstNSI RCATGY 1 cut(s) 1117
BstPAI GACNNNNGTC 1 cut(s) 1193
BstSCI CCNGG 6 cut(s) 118, 498, 511, 945, 1027, 1451
BstSFI CTRYAG 3 cut(s) 650, 1233, 1341
BstSLI GKGCMC 1 cut(s) 659
BstUI CGCG 2 cut(s) 22, 72
BstV1I GCAGC 7 cut(s) 190, 268, 271, 745, 1196, 1307, 1422
BstX2I RGATCY 1 cut(s) 1225
BstYI RGATCY 1 cut(s) 1225
BsuRI GGCC 1 cut(s) 792
BtgZI GCGATG 3 cut(s) 63, 138, 1275
BtsCI GGATG 5 cut(s) 136, 145, 160, 508, 529
BtsI GCAGTG 2 cut(s) 659, 978
BtsIMutI CAGTG 6 cut(s) 470, 659, 975, 978, 1056, 1150
Cac8I GCNNGC 5 cut(s) 534, 597, 680, 731, 1370
CaiI CAGNNNCTG 1 cut(s) 1402
CciI TCATGA 3 cut(s) 1185, 1327, 1387
Cfr13I GGNCC 2 cut(s) 509, 791
CseI GACGC 2 cut(s) 78, 1006
Csp6I GTAC 3 cut(s) 822, 966, 1135
CspCI CAANNNNNGTGG 2 cut(s) 648, 683
CviAII CATG 6 cut(s) 135, 1114, 1186, 1328, 1388, 1408
CviQI GTAC 3 cut(s) 822, 966, 1135
DdeI CTNAG 2 cut(s) 338, 1470
DpnI GATC 3 cut(s) 212, 708, 1227
DpnII GATC 3 cut(s) 210, 706, 1225
Eam1104I CTCTTC 2 cut(s) 828, 1470
EarI CTCTTC 2 cut(s) 828, 1470
Ecl136II GAGCTC 2 cut(s) 884, 929
Eco130I CCWWGG 3 cut(s) 256, 575, 1362
Eco24I GRGCYC 3 cut(s) 886, 931, 1374
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 509
Eco53kI GAGCTC 2 cut(s) 884, 929
EcoICRI GAGCTC 2 cut(s) 884, 929
EcoNI CCTNNNNNAGG 1 cut(s) 1344
EcoRII CCWGG 3 cut(s) 945, 1027, 1451
EcoT14I CCWWGG 3 cut(s) 256, 575, 1362
EcoT22I ATGCAT 1 cut(s) 136
EcoT38I GRGCYC 3 cut(s) 886, 931, 1374
ErhI CCWWGG 3 cut(s) 256, 575, 1362
Esp3I CGTCTC 1 cut(s) 719
FaeI CATG 6 cut(s) 138, 1117, 1189, 1331, 1391, 1411
FalI AAGNNNNNCTT 2 cut(s) 181, 213
FaqI GGGAC 3 cut(s) 361, 529, 1165
FatI CATG 6 cut(s) 134, 1113, 1185, 1327, 1387, 1407
Fnu4HI GCNGC 8 cut(s) 179, 282, 285, 641, 734, 1210, 1296, 1436
FokI GGATG 5 cut(s) 132, 143, 167, 515, 536
FriOI GRGCYC 3 cut(s) 886, 931, 1374
Fsp4HI GCNGC 8 cut(s) 179, 282, 285, 641, 734, 1210, 1296, 1436
FspBI CTAG 5 cut(s) 257, 576, 771, 963, 1104
GluI GCNGC 8 cut(s) 179, 282, 285, 641, 734, 1210, 1296, 1436
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 1 cut(s) 792
HapII CCGG 6 cut(s) 119, 291, 326, 500, 512, 1089
HgaI GACGC 2 cut(s) 78, 1006
Hin1II CATG 6 cut(s) 138, 1117, 1189, 1331, 1391, 1411
HindIII AAGCTT 1 cut(s) 692
HpaII CCGG 6 cut(s) 119, 291, 326, 500, 512, 1089
HphI GGTGA 4 cut(s) 40, 46, 864, 951
Hpy188I TCNGA 5 cut(s) 301, 472, 607, 1171, 1256
Hpy188III TCNNGA 8 cut(s) 214, 230, 366, 879, 1089, 1186, 1328, 1388
Hpy99I CGWCG 2 cut(s) 72, 895
HpyAV CCTTC 7 cut(s) 344, 368, 885, 1047, 1239, 1340, 1410
HpyCH4III ACNGT 2 cut(s) 34, 970
HpyCH4IV ACGT 1 cut(s) 1160
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 157, 166, 175, 293, 488, 623, 649
HpyF3I CTNAG 2 cut(s) 338, 1470
HpySE526I ACGT 1 cut(s) 1160
Hsp92II CATG 6 cut(s) 138, 1117, 1189, 1331, 1391, 1411
Kpn2I TCCGGA 1 cut(s) 1088
Kzo9I GATC 3 cut(s) 210, 706, 1225
LmnI GCTCC 4 cut(s) 278, 881, 934, 1432
Lsp1109I GCAGC 7 cut(s) 190, 268, 271, 745, 1196, 1307, 1422
LweI GCATC 7 cut(s) 105, 121, 305, 757, 878, 1328, 1469
MaeI CTAG 5 cut(s) 257, 576, 771, 963, 1104
MaeII ACGT 1 cut(s) 1160
MaeIII GTNAC 6 cut(s) 28, 80, 269, 1055, 1145, 1161
MalI GATC 3 cut(s) 212, 708, 1227
MbiI CCGCTC 1 cut(s) 169
MboI GATC 3 cut(s) 210, 706, 1225
MboII GAAGA 4 cut(s) 754, 845, 948, 1457
MflI RGATCY 1 cut(s) 1225
MhlI GDGCHC 4 cut(s) 659, 886, 931, 1374
MluCI AATT 7 cut(s) 245, 459, 741, 937, 951, 1078, 1198
MlyI GAGTC 6 cut(s) 25, 62, 88, 95, 172, 1142
MmeI TCCRAC 2 cut(s) 21, 1098
Mph1103I ATGCAT 1 cut(s) 136
MroI TCCGGA 1 cut(s) 1088
MroXI GAANNNNTTC 2 cut(s) 1350, 1420
MseI TTAA 4 cut(s) 444, 725, 1443, 1482
MslI CAYNNNNRTG 2 cut(s) 139, 1112
MspA1I CMGCKG 2 cut(s) 151, 1298
MspCI CTTAAG 1 cut(s) 443
MspI CCGG 6 cut(s) 119, 291, 326, 500, 512, 1089
MspR9I CCNGG 6 cut(s) 120, 500, 513, 947, 1029, 1453
MvaI CCWGG 3 cut(s) 947, 1029, 1453
MvnI CGCG 2 cut(s) 22, 72
MwoI GCNNNNNNNGC 8 cut(s) 69, 157, 166, 175, 293, 488, 623, 649
NciI CCSGG 3 cut(s) 120, 500, 513
NdeII GATC 3 cut(s) 210, 706, 1225
NlaIII CATG 6 cut(s) 138, 1117, 1189, 1331, 1391, 1411
NlaIV GGNNCC 2 cut(s) 64, 1227
NmeAIII GCCGAG 3 cut(s) 186, 981, 1437
NmuCI GTSAC 5 cut(s) 28, 80, 269, 1055, 1145
NsiI ATGCAT 1 cut(s) 136
NspI RCATGY 1 cut(s) 1117
NspV TTCGAA 1 cut(s) 687
PagI TCATGA 3 cut(s) 1185, 1327, 1387
PciI ACATGT 1 cut(s) 1113
PdmI GAANNNNTTC 2 cut(s) 1350, 1420
PfeI GAWTC 4 cut(s) 752, 863, 1382, 1421
PflMI CCANNNNNTGG 1 cut(s) 151
PfoI TCCNGGA 1 cut(s) 1027
PkrI GCNGC 8 cut(s) 180, 283, 286, 642, 735, 1211, 1297, 1437
PleI GAGTC 6 cut(s) 24, 61, 87, 95, 171, 1142
PpsI GAGTC 6 cut(s) 24, 61, 87, 95, 171, 1142
PscI ACATGT 1 cut(s) 1113
PshAI GACNNNNGTC 1 cut(s) 1193
Psp124BI GAGCTC 2 cut(s) 886, 931
Psp6I CCWGG 3 cut(s) 945, 1027, 1451
PspGI CCWGG 3 cut(s) 945, 1027, 1451
PspN4I GGNNCC 2 cut(s) 64, 1227
PspPI GGNCC 2 cut(s) 509, 791
PstI CTGCAG 1 cut(s) 654
PstNI CAGNNNCTG 1 cut(s) 1402
PsuI RGATCY 1 cut(s) 1225
RsaI GTAC 3 cut(s) 823, 967, 1136
RsaNI GTAC 3 cut(s) 822, 966, 1135
RseI CAYNNNNRTG 2 cut(s) 139, 1112
SacI GAGCTC 2 cut(s) 886, 931
SaqAI TTAA 4 cut(s) 444, 725, 1443, 1482
SatI GCNGC 8 cut(s) 179, 282, 285, 641, 734, 1210, 1296, 1436
Sau3AI GATC 3 cut(s) 210, 706, 1225
Sau96I GGNCC 2 cut(s) 509, 791
ScaI AGTACT 1 cut(s) 1136
SchI GAGTC 6 cut(s) 25, 62, 88, 95, 172, 1142
ScrFI CCNGG 6 cut(s) 120, 500, 513, 947, 1029, 1453
SduI GDGCHC 4 cut(s) 659, 886, 931, 1374
SfaNI GCATC 7 cut(s) 105, 121, 305, 757, 878, 1328, 1469
SfcI CTRYAG 3 cut(s) 650, 1233, 1341
SfuI TTCGAA 1 cut(s) 687
SinI GGWCC 1 cut(s) 509
SmiMI CAYNNNNRTG 2 cut(s) 139, 1112
SmlI CTYRAG 3 cut(s) 228, 308, 443
SmoI CTYRAG 3 cut(s) 228, 308, 443
Sse9I AATT 7 cut(s) 245, 459, 741, 937, 951, 1078, 1198
SspI AATATT 1 cut(s) 1447
SspMI CTAG 5 cut(s) 257, 576, 771, 963, 1104
SstI GAGCTC 2 cut(s) 886, 931
StyD4I CCNGG 6 cut(s) 118, 498, 511, 945, 1027, 1451
StyI CCWWGG 3 cut(s) 256, 575, 1362
TaaI ACNGT 2 cut(s) 34, 970
TaiI ACGT 1 cut(s) 1163
TaqI TCGA 3 cut(s) 99, 687, 765
TasI AATT 7 cut(s) 245, 459, 741, 937, 951, 1078, 1198
TatI WGTACW 2 cut(s) 965, 1134
TauI GCSGC 1 cut(s) 643
TfiI GAWTC 4 cut(s) 752, 863, 1382, 1421
Tru1I TTAA 4 cut(s) 444, 725, 1443, 1482
Tru9I TTAA 4 cut(s) 444, 725, 1443, 1482
TscAI CASTG 6 cut(s) 470, 659, 975, 985, 1056, 1150
TseFI GTSAC 5 cut(s) 28, 80, 269, 1055, 1145
TseI GCWGC 7 cut(s) 178, 281, 284, 733, 1209, 1295, 1435
Tsp45I GTSAC 5 cut(s) 28, 80, 269, 1055, 1145
TspDTI ATGAA 6 cut(s) 233, 332, 816, 846, 921, 1413
TspGWI ACGGA 1 cut(s) 747
TspRI CASTG 6 cut(s) 470, 659, 975, 985, 1056, 1150
Van91I CCANNNNNTGG 1 cut(s) 151
Vha464I CTTAAG 1 cut(s) 443
VpaK11BI GGWCC 1 cut(s) 509
XagI CCTNNNNNAGG 1 cut(s) 1344
XapI RAATTY 2 cut(s) 459, 951
XceI RCATGY 1 cut(s) 1117
XmaJI CCTAGG 2 cut(s) 256, 575
XmnI GAANNNNTTC 2 cut(s) 1350, 1420
XspI CTAG 5 cut(s) 257, 576, 771, 963, 1104
ZrmI AGTACT 1 cut(s) 1136
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.