Rorug02G0303500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007845
Physical Location & Seq
Reverse (-)
33981134 .. 33985310
4177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0303500.1

Sequence Viewer

Length: 1488 bp
ATGATATCCATGTACTCGAGAAATGGCAGTTTTGACGATGCACGCCATTTGCTTGCTAAAATGACAGAACCAAATCTTGTGAGCTATAATACTTTGGTTTCCGGTTTAGCACGTCATGGGTATCATAAAGAATCCATATCTCTCTTCCAAAGGATGCAACAGGATTGTGAATGTTTATTCTTGGACCAGTTCACGTTTGTAAGCATAGTGGGGACTTGTGCTTGCTTAGGTGCATTGGCACTGTTGCGTCAAGTTCATGGGGTGGCGATTGCAATTGGCTTGGAAATGAATATCATTGCTTGCAATTCTTTGGTCAATGCTTATGGAAAATGTGGTGAACCAGACACCTCATATTCTGTATTCAGTCGAATGCTAGAGAGGGACGTTGTGTCTTGGACCTCAATGATTGCTTCTTATGCACGGGCATCAAGAATGGATGACGCTTGTTGGGTATTTCATGGTATGCCTCTTAAGAACACAGTTTCTTGGACTGCTTTGATATCAGGTTTTGTACAAAGTGGGCATGGTAATTATGCTTTGGATCTTTTCCAGAAAATGCTGGAAGAGAGGATCCAGCCTAGCACAGTTACATATGTTAGCATTTTGAGTGCTTGTGCAGATCTAGCACTTAATGAAAGAGGTAAGCAGATTCATGGGCATATCATTAGAAGTAATAATGGAAGCTACTTGTCCAATGTATTTATTTGTAATGCTCTAGTTGACATGTACAGCAAATGTGGGGACATGAAATCGGGAAAAGCATTGTTTGAGATGATTCCTGAGAAGGATGTAATCTCTTGGAACTCTTTAATTACTGGATTTGCTCAGAATGGGCTGGGAGAGGAGTCACTTCTTGTTTTTAAGAGGATGGTAGAAACAAATGTGAAGCCTACTCATGTGACTTTTCTTTTGGTGTTATCTGCTTGTAGCCACACGGGTTTAGTTTCTGAAGGACTTCAAATTCTGCACTTAATGGAAAAGGGTTATGGTATTAAACCTAGGTCAAGTCACTATGCAATTGTGATTGATTTGCTCGGGAGGAAAAATAAACTGAAGGAAGCAATGGAGTTGATTGAGAGAGCTCCTCATGCATCAGATCATATTGGGATGTGGGGTGCACTTTTGGCTGCTTGTCGAGTCCATGGGAACTTGGCCCTAGCTAGGAGGGCTGCAGAAGCTCTTTTTGAGTTGGAGCCCCACAATGGTGCAAGATATGTTATGTTATCAAACATTTATGCCGCAGCTGGTAGATGGGCTGATGCCCATAAGGTCAGAAGGCTCATGGAAGAAAGAGGTTTGACAAAAGAGTCAGCTTATAGTTTGATTGAGCTGAGAAATGCAAGGCATGAGTTTGTAGCAAAAGACAAGGTCCACTGTCAGACAGGAGAGATGAATGATATGATTGGTAAATTAGCAGACCATATGAACGATGTAGGCAATCTTCCTTACATTTACAGCCTTTTGTCCGAGGATAATGGTTATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

495

Amino Acids

55.01

Weight (kDa)

6.85

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 23 - 52 1e-05 PPR repeat
PPR PF01535 27 - 54 8.5e-07 PPR repeat
PPR_2 PF13041 158 - 206 5e-12 PPR repeat family
PPR_3 PF13812 159 - 206 2.8e-07 Pentatricopeptide repeat domain
PPR PF01535 161 - 190 2.8e-06 PPR repeat
PPR_2 PF13041 262 - 310 3.1e-09 PPR repeat family
PPR PF01535 265 - 292 8.8e-07 PPR repeat
E_motif PF20431 381 - 442 5.7e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1306
AciI CCGC 1 cut(s) 1239
AclWI GGATC 3 cut(s) 549, 565, 578
AcsI RAATTY 1 cut(s) 960
AcuI CTGAAG 2 cut(s) 969, 1073
AfaI GTAC 3 cut(s) 14, 513, 728
AfiI CCNNNNNNNGG 2 cut(s) 1161, 1202
AflII CTTAAG 1 cut(s) 470
AflIII ACRYGT 1 cut(s) 723
AgsI TTSAA 1 cut(s) 959
AhdI GACNNNNNGTC 1 cut(s) 388
AjiI CACGTC 1 cut(s) 113
AleI CACNNNNGTG 1 cut(s) 1203
AluBI AGCT 8 cut(s) 84, 684, 1082, 1160, 1178, 1244, 1313, 1330
AluI AGCT 8 cut(s) 84, 684, 1082, 1160, 1178, 1244, 1313, 1330
Alw21I GWGCWC 2 cut(s) 1084, 1120
Alw44I GTGCAC 1 cut(s) 1116
AlwI GGATC 3 cut(s) 549, 565, 578
Ama87I CYCGRG 2 cut(s) 16, 1034
AoxI GGCC 1 cut(s) 1152
ApaLI GTGCAC 1 cut(s) 1116
ApeKI GCWGC 3 cut(s) 1127, 1169, 1241
ApoI RAATTY 1 cut(s) 960
ArsI GACNNNNNNTTYG 2 cut(s) 892, 924
Asp700I GAANNNNTTC 1 cut(s) 954
AspA2I CCTAGG 1 cut(s) 998
AspS9I GGNCC 4 cut(s) 184, 396, 1153, 1369
AsuHPI GGTGA 1 cut(s) 347
AvaI CYCGRG 2 cut(s) 16, 1034
AvaII GGWCC 3 cut(s) 184, 396, 1369
AvrII CCTAGG 1 cut(s) 998
BaeGI GKGCMC 1 cut(s) 1120
BamHI GGATCC 1 cut(s) 570
BanII GRGCYC 2 cut(s) 1084, 1197
BarI GAAGNNNNNNTAC 2 cut(s) 1425, 1457
Bbv12I GWGCWC 2 cut(s) 1084, 1120
BbvI GCAGC 3 cut(s) 1114, 1156, 1253
BccI CCATC 2 cut(s) 862, 1245
BfaI CTAG 7 cut(s) 374, 579, 623, 716, 999, 1157, 1161
BfmI CTRYAG 1 cut(s) 1170
BfrI CTTAAG 1 cut(s) 470
BglII AGATCT 1 cut(s) 619
BisI GCNGC 4 cut(s) 1128, 1170, 1239, 1242
BlnI CCTAGG 1 cut(s) 998
BlsI GCNGC 4 cut(s) 1129, 1171, 1240, 1243
Bme18I GGWCC 3 cut(s) 184, 396, 1369
BmeRI GACNNNNNGTC 1 cut(s) 388
BmeT110I CYCGRG 2 cut(s) 16, 1034
BmgBI CACGTC 1 cut(s) 113
BmgT120I GGNCC 4 cut(s) 184, 396, 1153, 1369
BmiI GGNNCC 2 cut(s) 572, 1194
BmsI GCATC 5 cut(s) 28, 144, 434, 1100, 1249
BplI GAGNNNNNCTC 2 cut(s) 1069, 1101
Bpu10I CCTNAGC 1 cut(s) 226
BsaJI CCNNGG 3 cut(s) 998, 1141, 1467
BsaWI WCCGGW 1 cut(s) 101
Bsc4I CCNNNNNNNGG 2 cut(s) 1161, 1202
Bse1I ACTGG 2 cut(s) 187, 820
Bse3DI GCAATG 2 cut(s) 294, 1068
BseDI CCNNGG 3 cut(s) 998, 1141, 1467
BseGI GGATG 5 cut(s) 159, 442, 793, 873, 1113
BseLI CCNNNNNNNGG 2 cut(s) 1161, 1202
BseMI GCAATG 2 cut(s) 294, 1068
BseMII CTCAG 3 cut(s) 771, 839, 1322
BseNI ACTGG 2 cut(s) 187, 820
BseRI GAGGAG 2 cut(s) 857, 1074
BseSI GKGCMC 1 cut(s) 1120
BseXI GCAGC 3 cut(s) 1114, 1156, 1253
BseYI CCCAGC 1 cut(s) 835
BsgI GTGCAG 2 cut(s) 636, 950
BshFI GGCC 1 cut(s) 1154
BsiHKAI GWGCWC 2 cut(s) 1084, 1120
BsiHKCI CYCGRG 2 cut(s) 16, 1034
BsiSI CCGG 1 cut(s) 102
BslFI GGGAC 3 cut(s) 226, 395, 755
BslI CCNNNNNNNGG 2 cut(s) 1161, 1202
BsmFI GGGAC 3 cut(s) 226, 395, 755
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 1 cut(s) 1154
BsoBI CYCGRG 2 cut(s) 16, 1034
Bsp1286I GDGCHC 3 cut(s) 1084, 1120, 1197
Bsp1407I TGTACA 2 cut(s) 511, 726
Bsp143I GATC 4 cut(s) 541, 570, 619, 1096
Bsp19I CCATGG 1 cut(s) 1141
BspACI CCGC 1 cut(s) 1239
BspANI GGCC 1 cut(s) 1154
BspCNI CTCAG 3 cut(s) 772, 838, 1323
BspLI GGNNCC 2 cut(s) 572, 1194
BspMAI CTGCAG 1 cut(s) 1174
BspPI GGATC 3 cut(s) 549, 565, 578
BspTI CTTAAG 1 cut(s) 470
BsrDI GCAATG 2 cut(s) 294, 1068
BsrGI TGTACA 2 cut(s) 511, 726
BsrI ACTGG 2 cut(s) 187, 820
BssECI CCNNGG 3 cut(s) 998, 1141, 1467
BssMI GATC 4 cut(s) 541, 570, 619, 1096
BssT1I CCWWGG 2 cut(s) 998, 1141
Bst4CI ACNGT 4 cut(s) 243, 481, 586, 1376
Bst6I CTCTTC 2 cut(s) 149, 558
BstAFI CTTAAG 1 cut(s) 470
BstAUI TGTACA 2 cut(s) 511, 726
BstC8I GCNNGC 4 cut(s) 43, 54, 223, 301
BstDEI CTNAG 4 cut(s) 226, 780, 825, 1331
BstDSI CCRYGG 1 cut(s) 1141
BstF5I GGATG 5 cut(s) 159, 442, 793, 873, 1113
BstKTI GATC 4 cut(s) 544, 573, 622, 1099
BstMBI GATC 4 cut(s) 541, 570, 619, 1096
BstMWI GCNNNNNNNGC 6 cut(s) 416, 623, 1088, 1124, 1166, 1175
BstNSI RCATGY 1 cut(s) 727
BstSFI CTRYAG 1 cut(s) 1170
BstSLI GKGCMC 1 cut(s) 1120
BstV1I GCAGC 3 cut(s) 1114, 1156, 1253
BstX2I RGATCY 3 cut(s) 541, 570, 619
BstYI RGATCY 3 cut(s) 541, 570, 619
BsuRI GGCC 1 cut(s) 1154
BtgI CCRYGG 1 cut(s) 1141
BtrI CACGTC 1 cut(s) 113
BtsCI GGATG 5 cut(s) 159, 442, 793, 873, 1113
BtsIMutI CAGTG 2 cut(s) 239, 1372
Cac8I GCNNGC 4 cut(s) 43, 54, 223, 301
Cfr13I GGNCC 4 cut(s) 184, 396, 1153, 1369
CseI GACGC 2 cut(s) 236, 449
Csp6I GTAC 3 cut(s) 13, 512, 727
CviQI GTAC 3 cut(s) 13, 512, 727
DdeI CTNAG 4 cut(s) 226, 780, 825, 1331
DpnI GATC 4 cut(s) 543, 572, 621, 1098
DpnII GATC 4 cut(s) 541, 570, 619, 1096
DrdI GACNNNNNNGTC 1 cut(s) 1306
DriI GACNNNNNGTC 1 cut(s) 388
DseDI GACNNNNNNGTC 1 cut(s) 1306
Eam1104I CTCTTC 2 cut(s) 149, 558
Eam1105I GACNNNNNGTC 1 cut(s) 388
EarI CTCTTC 2 cut(s) 149, 558
Ecl136II GAGCTC 1 cut(s) 1082
Eco130I CCWWGG 2 cut(s) 998, 1141
Eco24I GRGCYC 2 cut(s) 1084, 1197
Eco32I GATATC 2 cut(s) 6, 501
Eco47I GGWCC 3 cut(s) 184, 396, 1369
Eco53kI GAGCTC 1 cut(s) 1082
Eco57I CTGAAG 2 cut(s) 969, 1073
Eco88I CYCGRG 2 cut(s) 16, 1034
EcoICRI GAGCTC 1 cut(s) 1082
EcoRV GATATC 2 cut(s) 6, 501
EcoT14I CCWWGG 2 cut(s) 998, 1141
EcoT22I ATGCAT 1 cut(s) 1093
EcoT38I GRGCYC 2 cut(s) 1084, 1197
ErhI CCWWGG 2 cut(s) 998, 1141
FaqI GGGAC 3 cut(s) 226, 395, 755
FauNDI CATATG 2 cut(s) 592, 1422
Fnu4HI GCNGC 4 cut(s) 1128, 1170, 1239, 1242
FokI GGATG 5 cut(s) 166, 449, 800, 880, 1120
FriOI GRGCYC 2 cut(s) 1084, 1197
Fsp4HI GCNGC 4 cut(s) 1128, 1170, 1239, 1242
FspBI CTAG 7 cut(s) 374, 579, 623, 716, 999, 1157, 1161
GluI GCNGC 4 cut(s) 1128, 1170, 1239, 1242
GsaI CCCAGC 1 cut(s) 839
HaeIII GGCC 1 cut(s) 1154
HapII CCGG 1 cut(s) 102
HgaI GACGC 2 cut(s) 236, 449
HincII GTYRAC 1 cut(s) 721
HindII GTYRAC 1 cut(s) 721
HinfI GANTC 6 cut(s) 131, 649, 775, 845, 1137, 1307
HpaII CCGG 1 cut(s) 102
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 5 cut(s) 192, 338, 721, 1118, 1372
Hpy188I TCNGA 6 cut(s) 828, 949, 1096, 1274, 1380, 1468
Hpy188III TCNNGA 7 cut(s) 18, 429, 550, 753, 779, 1036, 1485
Hpy8I GTNNAC 5 cut(s) 192, 338, 721, 1118, 1372
HpyAV CCTTC 4 cut(s) 778, 944, 1048, 1269
HpyCH4III ACNGT 4 cut(s) 243, 481, 586, 1376
HpyCH4IV ACGT 3 cut(s) 112, 194, 384
HpyF10VI GCNNNNNNNGC 6 cut(s) 416, 623, 1088, 1124, 1166, 1175
HpyF3I CTNAG 4 cut(s) 226, 780, 825, 1331
HpySE526I ACGT 3 cut(s) 112, 194, 384
Kzo9I GATC 4 cut(s) 541, 570, 619, 1096
LmnI GCTCC 2 cut(s) 1087, 1192
Lsp1109I GCAGC 3 cut(s) 1114, 1156, 1253
LweI GCATC 5 cut(s) 28, 144, 434, 1100, 1249
MaeI CTAG 7 cut(s) 374, 579, 623, 716, 999, 1157, 1161
MaeII ACGT 3 cut(s) 112, 194, 384
MaeIII GTNAC 4 cut(s) 586, 846, 898, 1007
MalI GATC 4 cut(s) 543, 572, 621, 1098
MboI GATC 4 cut(s) 541, 570, 619, 1096
MboII GAAGA 4 cut(s) 136, 575, 1298, 1433
MfeI CAATTG 2 cut(s) 273, 1017
MflI RGATCY 3 cut(s) 541, 570, 619
MhlI GDGCHC 3 cut(s) 1084, 1120, 1197
MluCI AATT 7 cut(s) 273, 304, 529, 810, 960, 1017, 1409
MlyI GAGTC 3 cut(s) 854, 1146, 1316
MmeI TCCRAC 1 cut(s) 1170
Mph1103I ATGCAT 1 cut(s) 1093
MroXI GAANNNNTTC 1 cut(s) 954
MseI TTAA 6 cut(s) 471, 630, 809, 861, 971, 993
MslI CAYNNNNRTG 1 cut(s) 1203
MspA1I CMGCKG 1 cut(s) 1244
MspCI CTTAAG 1 cut(s) 470
MspI CCGG 1 cut(s) 102
MunI CAATTG 2 cut(s) 273, 1017
Mva1269I GAATGC 1 cut(s) 375
MwoI GCNNNNNNNGC 6 cut(s) 416, 623, 1088, 1124, 1166, 1175
NcoI CCATGG 1 cut(s) 1141
NdeI CATATG 2 cut(s) 592, 1422
NdeII GATC 4 cut(s) 541, 570, 619, 1096
NlaIV GGNNCC 2 cut(s) 572, 1194
NmuCI GTSAC 3 cut(s) 846, 898, 1007
NsiI ATGCAT 1 cut(s) 1093
NspI RCATGY 1 cut(s) 727
OliI CACNNNNGTG 1 cut(s) 1203
PaeR7I CTCGAG 1 cut(s) 16
PciI ACATGT 1 cut(s) 723
PctI GAATGC 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 954
PfeI GAWTC 3 cut(s) 131, 649, 775
PflFI GACNNNGTC 1 cut(s) 1367
PkrI GCNGC 4 cut(s) 1129, 1171, 1240, 1243
PleI GAGTC 3 cut(s) 853, 1145, 1315
PpsI GAGTC 3 cut(s) 853, 1145, 1315
PscI ACATGT 1 cut(s) 723
Psp124BI GAGCTC 1 cut(s) 1084
PspFI CCCAGC 1 cut(s) 835
PspN4I GGNNCC 2 cut(s) 572, 1194
PspPI GGNCC 4 cut(s) 184, 396, 1153, 1369
PstI CTGCAG 1 cut(s) 1174
PsuI RGATCY 3 cut(s) 541, 570, 619
PsyI GACNNNGTC 1 cut(s) 1367
PvuII CAGCTG 1 cut(s) 1244
RsaI GTAC 3 cut(s) 14, 513, 728
RsaNI GTAC 3 cut(s) 13, 512, 727
RseI CAYNNNNRTG 1 cut(s) 1203
SacI GAGCTC 1 cut(s) 1084
SaqAI TTAA 6 cut(s) 471, 630, 809, 861, 971, 993
SatI GCNGC 4 cut(s) 1128, 1170, 1239, 1242
Sau3AI GATC 4 cut(s) 541, 570, 619, 1096
Sau96I GGNCC 4 cut(s) 184, 396, 1153, 1369
SchI GAGTC 3 cut(s) 854, 1146, 1316
SduI GDGCHC 3 cut(s) 1084, 1120, 1197
SfaNI GCATC 5 cut(s) 28, 144, 434, 1100, 1249
SfcI CTRYAG 1 cut(s) 1170
Sfr274I CTCGAG 1 cut(s) 16
SinI GGWCC 3 cut(s) 184, 396, 1369
SlaI CTCGAG 1 cut(s) 16
SmiMI CAYNNNNRTG 1 cut(s) 1203
SmlI CTYRAG 2 cut(s) 16, 470
SmoI CTYRAG 2 cut(s) 16, 470
Sse9I AATT 7 cut(s) 273, 304, 529, 810, 960, 1017, 1409
SsiI CCGC 1 cut(s) 1239
SspMI CTAG 7 cut(s) 374, 579, 623, 716, 999, 1157, 1161
SstI GAGCTC 1 cut(s) 1084
StyI CCWWGG 2 cut(s) 998, 1141
TaaI ACNGT 4 cut(s) 243, 481, 586, 1376
TaiI ACGT 3 cut(s) 115, 197, 387
TaqI TCGA 3 cut(s) 17, 367, 1135
TasI AATT 7 cut(s) 273, 304, 529, 810, 960, 1017, 1409
TatI WGTACW 3 cut(s) 12, 511, 726
TauI GCSGC 1 cut(s) 1241
TfiI GAWTC 3 cut(s) 131, 649, 775
Tru1I TTAA 6 cut(s) 471, 630, 809, 861, 971, 993
Tru9I TTAA 6 cut(s) 471, 630, 809, 861, 971, 993
TscAI CASTG 2 cut(s) 246, 1379
TseFI GTSAC 3 cut(s) 846, 898, 1007
TseI GCWGC 3 cut(s) 1127, 1169, 1241
Tsp45I GTSAC 3 cut(s) 846, 898, 1007
TspDTI ATGAA 8 cut(s) 245, 302, 446, 641, 648, 761, 1406, 1439
TspRI CASTG 2 cut(s) 246, 1379
Tth111I GACNNNGTC 1 cut(s) 1367
Vha464I CTTAAG 1 cut(s) 470
VneI GTGCAC 1 cut(s) 1116
VpaK11BI GGWCC 3 cut(s) 184, 396, 1369
XapI RAATTY 1 cut(s) 960
XceI RCATGY 1 cut(s) 727
XhoI CTCGAG 1 cut(s) 16
XmaJI CCTAGG 1 cut(s) 998
XmnI GAANNNNTTC 1 cut(s) 954
XspI CTAG 7 cut(s) 374, 579, 623, 716, 999, 1157, 1161
Zsp2I ATGCAT 1 cut(s) 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.